SitesBLAST
Comparing AZOBR_RS01680 FitnessBrowser__azobra:AZOBR_RS01680 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5dn1A Crystal structure of phosphoribosyl isomerase a from streptomyces coelicolor (see paper)
40% identity, 90% coverage: 3:226/249 of query aligns to 6:226/240 of 5dn1A
- active site: D11 (= D8), D130 (= D129)
- binding aminoimidazole 4-carboxamide ribonucleotide: L20 (= L17), G23 (= G20), V52 (= V50), L54 (= L52), A57 (= A55), F58 (= F56), S81 (≠ G80), G82 (= G81), G83 (= G82), R85 (= R84), N102 (≠ I101), G104 (= G103), T105 (= T104), D130 (= D129), G140 (= G139), W141 (= W140)
P16250 Phosphoribosyl isomerase A; 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; N-(5'-phosphoribosyl)anthranilate isomerase; PRAI; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; EC 5.3.1.16; EC 5.3.1.24 from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see paper)
40% identity, 90% coverage: 3:226/249 of query aligns to 6:226/240 of P16250
- D11 (= D8) mutation to A: No activity.
- R19 (= R16) mutation to A: No effect on activity toward PRA. No activity toward ProFAR.
- S81 (≠ G80) mutation to T: No activity toward PRA. Almost no effect on activity toward ProFAR.
- D130 (= D129) mutation to A: Very low activity toward PRA. No activity toward ProFAR.; mutation to Q: No activity.
- T166 (= T166) mutation to A: No activity.
- D171 (= D171) mutation to A: Low activity toward PRA. No activity toward ProFAR.
3zs4A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase with bound prfar
37% identity, 95% coverage: 1:236/249 of query aligns to 3:239/244 of 3zs4A
- active site: D10 (= D8), D129 (= D129)
- binding phosphoric acid mono-[5-({[5-carbamoyl-3-(5-phosphonooxy-5-deoxy-ribofuranosyl)- 3h-imidazol-4-ylamino]-methyl}-amino)-2,3,4-trihydroxy-pentyl] ester: A8 (= A6), D10 (= D8), R18 (= R16), G22 (= G20), H49 (= H48), V51 (= V50), L53 (= L52), A56 (= A55), F57 (= F56), S80 (≠ G80), G81 (= G81), G82 (= G82), I83 (= I83), R84 (= R84), V102 (≠ L102), G103 (= G103), T104 (= T104), D129 (= D129), G143 (= G139), W144 (= W140), D174 (= D171), G175 (= G172), S199 (= S196), G200 (= G197), G201 (= G198), I223 (= I220), V224 (≠ C221), G225 (= G222), K226 (≠ R223)
2y88A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase (variant d11n) with bound prfar (see paper)
37% identity, 95% coverage: 1:236/249 of query aligns to 3:239/244 of 2y88A
- active site: N10 (≠ D8), D129 (= D129)
- binding [(2r,3s,4r,5r)-5-[4-aminocarbonyl-5-[[(z)-[(3r,4r)-3,4-dihydroxy-2-oxo-5-phosphonooxy-pentyl]iminomethyl]amino]imidazol-1-yl]-3,4-dihydroxy-oxolan-2-yl]methyl dihydrogen phosphate: A8 (= A6), N10 (≠ D8), R18 (= R16), G22 (= G20), H49 (= H48), V51 (= V50), L53 (= L52), A56 (= A55), F57 (= F56), S80 (≠ G80), G81 (= G81), G82 (= G82), I83 (= I83), R84 (= R84), G103 (= G103), T104 (= T104), D129 (= D129), G143 (= G139), W144 (= W140), D174 (= D171), G175 (= G172), S199 (= S196), G200 (= G197), G201 (= G198), I223 (= I220), V224 (≠ C221), G225 (= G222), K226 (≠ R223)
4tx9A Crystal structure of hisap from streptomyces sviceus with degraded profar (see paper)
41% identity, 90% coverage: 3:226/249 of query aligns to 11:231/246 of 4tx9A
- active site: D16 (= D8), D135 (= D129)
- binding aminoimidazole 4-carboxamide ribonucleotide: G28 (= G20), V57 (= V50), L59 (= L52), A62 (= A55), F63 (= F56), S86 (≠ G80), G87 (= G81), G88 (= G82), I89 (= I83), R90 (= R84), L108 (= L102), G109 (= G103), T110 (= T104), D135 (= D129), G145 (= G139), W146 (= W140)
2y85A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase with bound rcdrp (see paper)
36% identity, 95% coverage: 1:236/249 of query aligns to 3:230/234 of 2y85A
- active site: D10 (= D8), D120 (= D129)
- binding 1-(o-carboxy-phenylamino)-1-deoxy-d-ribulose-5-phosphate: A8 (= A6), D10 (= D8), H40 (= H48), V42 (= V50), L44 (= L52), A47 (= A55), S71 (≠ G80), R133 (≠ A138), D165 (= D171), G166 (= G172), S190 (= S196), G191 (= G197), G192 (= G198), I214 (= I220), V215 (≠ C221), G216 (= G222), K217 (≠ R223)
5abtA S.Enterica hisa mutant d7n, g102a, v106m, d176a
33% identity, 95% coverage: 2:237/249 of query aligns to 1:240/246 of 5abtA
- active site: N7 (≠ D8), D129 (= D129)
- binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: N7 (≠ D8), L16 (= L17), G19 (= G20), H47 (= H48), V49 (= V50), L51 (= L52), G80 (= G81), G81 (= G82), V82 (≠ I83), R83 (= R84), V100 (≠ I101), I101 (≠ L102), A102 (≠ G103), S103 (≠ T104), D129 (= D129), G144 (= G139), W145 (= W140), A176 (≠ D171), G177 (= G172), S202 (= S196), G203 (= G197), G204 (= G198), I223 (= I220), V224 (≠ C221), G225 (= G222), R226 (= R223)
Q9X0C7 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; EC 5.3.1.16 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
30% identity, 92% coverage: 1:228/249 of query aligns to 1:229/241 of Q9X0C7
- D8 (= D8) mutation to N: Loss of activity.
- H48 (= H48) mutation to A: Decrease in activity.
- D51 (= D51) mutation to N: Decrease in activity.
- R83 (= R84) mutation to N: Decrease in activity.
- D127 (= D129) mutation to N: Almost no activity.
- T164 (= T166) mutation to A: Strong decrease in activity.
5ab3A S.Enterica hisa mutant d7n, d10g, dup13-15, q24l, g102a (see paper)
32% identity, 95% coverage: 2:237/249 of query aligns to 1:237/241 of 5ab3A
- active site: N7 (≠ D8), D132 (= D129)
- binding [(2r,3s,4r,5r)-5-[4-aminocarbonyl-5-[[(z)-[(3r,4r)-3,4-dihydroxy-2-oxo-5-phosphonooxy-pentyl]iminomethyl]amino]imidazol-1-yl]-3,4-dihydroxy-oxolan-2-yl]methyl dihydrogen phosphate: A5 (= A6), N7 (≠ D8), V16 (≠ L17), R18 (= R19), Y24 (≠ M22), V52 (= V50), L54 (= L52), G83 (= G81), G84 (= G82), V103 (≠ I101), I104 (≠ L102), A105 (≠ G103), S106 (≠ T104), A130 (≠ G127), D132 (= D129), D173 (= D171), G174 (= G172), S199 (= S196), G200 (= G197), G201 (= G198), I220 (= I220), V221 (≠ C221), G222 (= G222), R223 (= R223)
1h5yB Hisf protein from pyrobaculum aerophilum (see paper)
33% identity, 82% coverage: 3:205/249 of query aligns to 7:213/253 of 1h5yB
- active site: D12 (= D8), D133 (= D129)
- binding glycerol: K22 (≠ R19), L53 (≠ V50), N106 (≠ G103), A131 (≠ G127), D133 (= D129), D133 (= D129), G147 (= G139), G148 (≠ W140), T174 (= T166), I176 (= I168), D179 (= D171)
- binding phosphate ion: G84 (= G81), G85 (= G82), V86 (≠ I83), N106 (≠ G103), T107 (= T104), D179 (= D171), G180 (= G172), G205 (= G197), G206 (= G198)
Sites not aligning to the query:
7ac8A Molecular basis for the unique allosteric activation mechanism of the heterodimeric imidazole glycerol phosphate synthase complex. (see paper)
26% identity, 82% coverage: 3:206/249 of query aligns to 6:211/252 of 7ac8A
- active site: D11 (= D8), D130 (= D129)
- binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: L50 (≠ V50), I52 (≠ L52), G81 (= G81), G82 (= G82), I102 (≠ L102), N103 (≠ G103), T104 (= T104), D130 (= D129), S144 (≠ G139), T171 (= T166), D176 (= D171), G177 (= G172), S201 (= S196), G202 (= G197), G203 (= G198)
Sites not aligning to the query:
- binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: 222, 223, 224, 225
Q9X0C6 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
26% identity, 82% coverage: 3:206/249 of query aligns to 6:211/253 of Q9X0C6
- C9 (≠ A6) mutation to A: No change in activity.
- D11 (= D8) mutation to X: Loss of activity.
- K19 (≠ R19) mutation to S: Decrease in activity.
- D51 (= D51) mutation to N: No change in activity.
- N103 (≠ G103) mutation to A: No change in activity.
- D130 (= D129) mutation D->A,C,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y: Complete loss of activity.; mutation to E: Weak activity.
- D176 (= D171) mutation to N: Decrease in activity.
- D183 (≠ N178) mutation to N: No change in activity.
1gpwA Structural evidence for ammonia tunneling across the (beta/alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex. (see paper)
26% identity, 78% coverage: 13:206/249 of query aligns to 8:211/253 of 1gpwA
Sites not aligning to the query:
7qc8A Imidazole glycerol phosphate synthase subunit HisF (see paper)
27% identity, 82% coverage: 3:206/249 of query aligns to 6:211/250 of 7qc8A
3zr4E Structural evidence for ammonia tunneling across the (beta-alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex (see paper)
26% identity, 82% coverage: 3:206/249 of query aligns to 6:202/244 of 3zr4E
Sites not aligning to the query:
2wjzE Crystal structure of (hish) k181a y138a mutant of imidazoleglycerolphosphate synthase (hish hisf) which displays constitutive glutaminase activity (see paper)
25% identity, 82% coverage: 3:206/249 of query aligns to 6:197/237 of 2wjzE
4ewnD Structure of hisf-d130v+d176v with bound rcdrp (see paper)
25% identity, 82% coverage: 3:206/249 of query aligns to 5:204/243 of 4ewnD
Sites not aligning to the query:
5d2tA Directed evolutionary changes in kemp eliminase ke07 - crystal 3 wild type
24% identity, 82% coverage: 3:206/249 of query aligns to 4:209/251 of 5d2tA
Sites not aligning to the query:
6dnjA Directed evolutionary changes in kemp eliminase ke07 - crystal 28 round 5 (see paper)
24% identity, 82% coverage: 3:206/249 of query aligns to 5:210/250 of 6dnjA