SitesBLAST
Comparing AZOBR_RS02405 FitnessBrowser__azobra:AZOBR_RS02405 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7rbxC Crystal structure of isocitrate lyase and phosphorylmutase:isocitrate lyase from brucella melitensis biovar abortus 2308 bound to itaconic acid (see paper)
73% identity, 96% coverage: 15:424/425 of query aligns to 14:423/425 of 7rbxC
6lrtA Crystal structure of isocitrate lyase (caur_3889) from chloroflexus aurantiacus in complex with isocitrate and manganese ion
74% identity, 96% coverage: 15:424/425 of query aligns to 18:423/423 of 6lrtA
7cmyC Isocitrate lyase from bacillus cereus atcc 14579 in complex with magnessium ion, glyoxylate, and succinate
71% identity, 96% coverage: 15:424/425 of query aligns to 17:417/417 of 7cmyC
P9WKK7 Isocitrate lyase; ICL; Isocitrase; Isocitratase; EC 4.1.3.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
65% identity, 97% coverage: 15:425/425 of query aligns to 22:428/428 of P9WKK7
- SGW 91:93 (= SGW 84:86) binding
- D153 (= D145) binding
- C191 (= C183) mutation to S: Adopts a conformation almost identical to the wild-type.
- GH 192:193 (= GH 184:185) binding
- R228 (= R220) binding
- NCSPS 313:317 (= NCSPS 309:313) binding
- K334 (≠ R330) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
- T347 (= T343) binding
6c4aA Crystal structure of 3-nitropropionate modified isocitrate lyase from mycobacterium tuberculosis with pyruvate (see paper)
65% identity, 96% coverage: 15:424/425 of query aligns to 23:428/428 of 6c4aA
- active site: Y90 (= Y82), D109 (= D101), D154 (= D145), E156 (= E147), H181 (= H172), E183 (= E174), C192 (= C183), H194 (= H185), R229 (= R220), E286 (= E281), Q309 (≠ K304), S316 (= S311), S318 (= S313)
- binding 3-nitropropanoic acid: Y357 (= Y352), S358 (= S353), R380 (≠ A375)
- binding magnesium ion: A277 (= A272), A280 (≠ S275), Q309 (≠ K304)
- binding pyruvic acid: Y90 (= Y82), S92 (= S84), G93 (= G85), W94 (= W86), D154 (= D145), C192 (= C183), R229 (= R220), W284 (= W279), T348 (= T343)
7rb1A Isocitrate lyase-1 from mycobacterium tuberculosis covalently modified by 5-descarboxy-5-nitro-d-isocitric acid (see paper)
65% identity, 96% coverage: 15:424/425 of query aligns to 22:427/427 of 7rb1A
- binding dihydroxyacetic acid: Y89 (= Y82), S91 (= S84), W93 (= W86), D153 (= D145), R228 (= R220), T347 (= T343)
- binding (3E)-3-(hydroxyimino)propanoic acid: C191 (= C183), G192 (= G184), H193 (= H185), R228 (= R220), S315 (= S311), S317 (= S313), T347 (= T343)
- binding magnesium ion: A276 (= A272), A279 (≠ S275), Q308 (≠ K304)
6wsiA Intact cis-2,3-epoxysuccinic acid bound to isocitrate lyase-1 from mycobacterium tuberculosis (see paper)
65% identity, 96% coverage: 15:424/425 of query aligns to 22:427/427 of 6wsiA
- active site: Y89 (= Y82), D108 (= D101), D153 (= D145), E155 (= E147), H180 (= H172), E182 (= E174), C191 (= C183), H193 (= H185), R228 (= R220), E285 (= E281), Q308 (≠ K304), S315 (= S311), S317 (= S313)
- binding magnesium ion: A276 (= A272), A279 (≠ S275), Q308 (≠ K304)
- binding (2R,3S)-oxirane-2,3-dicarboxylic acid: C191 (= C183), G192 (= G184), H193 (= H185), R228 (= R220), E285 (= E281), N313 (= N309), S315 (= S311), S317 (= S313), T347 (= T343)
6vb9A Covalent adduct of cis-2,3-epoxysuccinic acid with isocitrate lyase-1 from mycobacterium tuberculosis (see paper)
65% identity, 96% coverage: 15:424/425 of query aligns to 22:427/427 of 6vb9A
- active site: Y89 (= Y82), D108 (= D101), D153 (= D145), E155 (= E147), H180 (= H172), E182 (= E174), C191 (= C183), H193 (= H185), R228 (= R220), E285 (= E281), Q308 (≠ K304), S315 (= S311), S317 (= S313)
- binding magnesium ion: A276 (= A272), A279 (≠ S275), Q308 (≠ K304)
- binding oxalic acid: Y89 (= Y82), S91 (= S84), G92 (= G85), W93 (= W86), D153 (= D145), C191 (= C183), R228 (= R220), W283 (= W279), T347 (= T343)
5dqlA Crystal structure of 2-vinyl glyoxylate modified isocitrate lyase from mycobacterium tuberculosis (see paper)
65% identity, 96% coverage: 15:424/425 of query aligns to 22:427/427 of 5dqlA
- active site: Y89 (= Y82), D108 (= D101), D153 (= D145), E155 (= E147), H180 (= H172), E182 (= E174), C191 (= C183), H193 (= H185), R228 (= R220), E285 (= E281), Q308 (≠ K304), S315 (= S311), S317 (= S313)
- binding magnesium ion: A276 (= A272), A279 (≠ S275), Q308 (≠ K304)
- binding 4-hydroxy-2-oxobutanoic acid: W93 (= W86), D108 (= D101), C191 (= C183), H193 (= H185), S315 (= S311), S317 (= S313), T347 (= T343), L348 (= L344)
6xppA Crystal structure of itaconate modified mycobaterium tuberculosis isocitrate lyase (see paper)
65% identity, 96% coverage: 15:424/425 of query aligns to 21:426/426 of 6xppA
- active site: Y88 (= Y82), D107 (= D101), D152 (= D145), E154 (= E147), H179 (= H172), E181 (= E174), C190 (= C183), H192 (= H185), R227 (= R220), E284 (= E281), Q307 (≠ K304), S314 (= S311), S316 (= S313)
- binding 2-methylidenebutanedioic acid: W92 (= W86), C190 (= C183), H192 (= H185), R227 (= R220), N312 (= N309), S314 (= S311), S316 (= S313), T346 (= T343)
- binding magnesium ion: A275 (= A272), A278 (≠ S275), Q307 (≠ K304)
1f8iA Crystal structure of isocitrate lyase:nitropropionate:glyoxylate complex from mycobacterium tuberculosis (see paper)
65% identity, 96% coverage: 15:424/425 of query aligns to 22:427/427 of 1f8iA
- active site: Y89 (= Y82), D108 (= D101), D153 (= D145), E155 (= E147), H180 (= H172), E182 (= E174), S191 (≠ C183), H193 (= H185), R228 (= R220), E285 (= E281), Q308 (≠ K304), S315 (= S311), S317 (= S313)
- binding glyoxylic acid: Y89 (= Y82), S91 (= S84), W93 (= W86), D153 (= D145), T347 (= T343)
P0A9G6 Isocitrate lyase; ICL; Isocitrase; Isocitratase; EC 4.1.3.1 from Escherichia coli (strain K12) (see 3 papers)
62% identity, 96% coverage: 15:424/425 of query aligns to 19:434/434 of P0A9G6
- SGW 91:93 (= SGW 84:86) binding
- D157 (= D145) binding
- C195 (= C183) active site, Proton acceptor; mutation to A: Large decrease in activity.; mutation to S: Large decrease in activity.
- A219 (= A207) mutation to C: Isocitrate lyase activity is reduced compared to the wild-type.
- R232 (= R220) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1igwC Crystal structure of the isocitrate lyase from the a219c mutant of escherichia coli (see paper)
62% identity, 92% coverage: 15:407/425 of query aligns to 18:416/416 of 1igwC
- active site: Y88 (= Y82), D107 (= D101), D156 (= D145), E158 (= E147), H183 (= H172), E185 (= E174), C194 (= C183), R231 (= R220), E288 (= E281), K311 (= K304), S318 (= S311), S320 (= S313)
- binding pyruvic acid: S90 (= S84), G91 (= G85), W92 (= W86), D156 (= D145), R231 (= R220), T350 (= T343)
1igwA Crystal structure of the isocitrate lyase from the a219c mutant of escherichia coli (see paper)
59% identity, 92% coverage: 15:407/425 of query aligns to 18:396/396 of 1igwA
- active site: Y88 (= Y82), D107 (= D101), D156 (= D145), E158 (= E147), H183 (= H172), E185 (= E174), C194 (= C183), R227 (= R220), E284 (= E281), K307 (= K304)
- binding pyruvic acid: S90 (= S84), W92 (= W86), D156 (= D145), R227 (= R220), T330 (= T343)
5e9gD Structural insights of isocitrate lyases from magnaporthe oryzae (see paper)
33% identity, 92% coverage: 15:407/425 of query aligns to 31:486/486 of 5e9gD
- active site: Y100 (= Y82), D119 (= D101), D173 (= D145), D175 (≠ E147), H200 (= H172), E202 (= E174), C211 (= C183), H213 (= H185), R248 (= R220), E363 (= E281), Q386 (≠ K304), S393 (= S311), S395 (= S313)
- binding glyoxylic acid: Y100 (= Y82), S102 (= S84), G103 (= G85), W104 (= W86), D173 (= D145), H200 (= H172), R248 (= R220), T424 (= T343)
- binding glycerol: C211 (= C183), G212 (= G184), H213 (= H185), R248 (= R220)
5e9fD Structural insights of isocitrate lyases from magnaporthe oryzae (see paper)
34% identity, 92% coverage: 15:406/425 of query aligns to 30:453/453 of 5e9fD
- active site: Y99 (= Y82), D118 (= D101), D172 (= D145), D174 (≠ E147), H199 (= H172), E201 (= E174), R240 (= R220), E330 (= E281), Q353 (≠ K304), S360 (= S311), S362 (= S313)
- binding magnesium ion: D118 (= D101), D172 (= D145)
6edwC Crystal structure of mycobacterium tuberculosis icl2 in the apo form (see paper)
37% identity, 55% coverage: 7:241/425 of query aligns to 14:262/724 of 6edwC
Sites not aligning to the query:
6edzA Crystal structure of mycobacterium tuberculosis icl2 in complex with acetyl-coa, form i (see paper)
37% identity, 55% coverage: 7:241/425 of query aligns to 14:262/733 of 6edzA
Sites not aligning to the query:
- active site: 437, 460, 467, 469
- binding acetyl coenzyme *a: 643, 644, 645, 646, 652, 653, 655, 657, 658, 679, 681, 682, 684, 688, 692, 732
6edwB Crystal structure of mycobacterium tuberculosis icl2 in the apo form (see paper)
37% identity, 55% coverage: 7:241/425 of query aligns to 14:262/746 of 6edwB
Sites not aligning to the query:
7ebeA Crystal structure of isocitrate lyase-1 from candida albicans (see paper)
37% identity, 53% coverage: 15:241/425 of query aligns to 30:268/544 of 7ebeA
- active site: Y99 (= Y82), D118 (= D101), D172 (= D145), D174 (≠ E147), H199 (= H172), E201 (= E174), C210 (= C183), H212 (= H185), R247 (= R220)
- binding magnesium ion: G102 (= G85), W103 (= W86), D172 (= D145)
Sites not aligning to the query:
Query Sequence
>AZOBR_RS02405 FitnessBrowser__azobra:AZOBR_RS02405
MSLDEKTKAALRAARFEGIKRDYTQDDVKRLSGSVKIEYTLAEMGAQRLWELLNTEPYIN
TLGALTGNQAMQAVKAGLKAIYLSGWQVAGDANLAGQMYPDQSLYPANSVPAVVERINNT
FKRADEIQTAEGKGDTYWFAPIIADAEAGFGGPLNVFELMKAMIKAGASGVHFEDQLASE
KKCGHLGGKVLIPTQQHIRTLNAARLAADTMGTSTIVLCRTDAESAQLITSDVDERDHPF
IDFDAGRTSEGFFRLKKGTGVDHCIARGLSYAPYSDLLWWETSRPNLEEAKRFAEAIRKE
FPNKLLAYNCSPSFNWKANLDEADIAKFQREIGAMGYKFQFVTLAGFHSLNYSAFKLAKG
YAARGMAAYSELQEAEFAAEAEGYTATKHQREVGTGYFDAVATAISGGQSSTTAYKDSTE
ADQFH
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory