SitesBLAST
Comparing AZOBR_RS04900 FitnessBrowser__azobra:AZOBR_RS04900 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2o56A Crystal structure of a member of the enolase superfamily from salmonella typhimurium
35% identity, 98% coverage: 1:379/386 of query aligns to 2:385/392 of 2o56A
- active site: K165 (= K155), D167 (≠ Y157), M171 (≠ R161), L186 (≠ A176), E214 (≠ D205), H216 (≠ A207), E240 (= E231), G265 (= G256), E266 (= E257), Q287 (= Q278), D289 (= D280), H316 (= H307), E341 (= E332), A346 (≠ W337)
- binding magnesium ion: E214 (≠ D205), E240 (= E231), E266 (= E257)
2ox4C Crystal structure of putative dehydratase from zymomonas mobilis zm4
34% identity, 98% coverage: 1:378/386 of query aligns to 2:384/397 of 2ox4C
- active site: K165 (= K155), D167 (≠ Y157), E214 (≠ D205), H216 (≠ A207), E240 (= E231), G265 (= G256), E266 (= E257), Q287 (= Q278), D289 (= D280), H316 (= H307), E341 (= E332)
- binding magnesium ion: E214 (≠ D205), E240 (= E231), E266 (= E257)
4e6mA Crystal structure of putative dehydratase protein from salmonella enterica subsp. Enterica serovar typhimurium (salmonella typhimurium)
35% identity, 95% coverage: 17:381/386 of query aligns to 25:396/401 of 4e6mA
- active site: L45 (≠ T37), G48 (= G40), K169 (= K155), D171 (≠ Y157), I175 (= I162), E223 (≠ D205), H225 (≠ A207), E249 (= E231), G274 (= G256), E275 (= E257), R276 (= R258), Q296 (= Q278), D298 (= D280), H325 (= H307), C327 (= C309), E350 (= E332), A355 (= A338)
- binding magnesium ion: E223 (≠ D205), E249 (= E231), E275 (= E257)
Q6BF17 D-galactonate dehydratase; GalD; EC 4.2.1.6 from Escherichia coli (strain K12)
33% identity, 98% coverage: 1:379/386 of query aligns to 1:359/382 of Q6BF17
- H185 (≠ A207) mutation H->N,Q: Loss of activity.
- H285 (= H307) mutation to N: Loss of activity.
- E310 (= E332) mutation to Q: Loss of activity.
3rraB Crystal structure of enolase prk14017 (target efi-500653) from ralstonia pickettii 12j with magnesium bound
33% identity, 98% coverage: 1:379/386 of query aligns to 2:354/379 of 3rraB
- active site: I35 (≠ T37), R38 (≠ C41), Y118 (= Y125), K145 (≠ Q163), N147 (= N165), E151 (≠ R169), D184 (= D205), H186 (≠ A207), E210 (= E231), G235 (= G256), E236 (= E257), R237 (= R258), Q257 (= Q278), D259 (= D280), H286 (= H307), P288 (≠ C309), E311 (= E332)
- binding magnesium ion: D184 (= D205), E210 (= E231), E236 (= E257)
3rcyF Crystal structure of mandelate racemase/muconate lactonizing enzyme- like protein from roseovarius sp. Tm1035
29% identity, 93% coverage: 19:378/386 of query aligns to 24:375/397 of 3rcyF
- active site: K165 (≠ R161), D167 (≠ Q163), R175 (= R169), G208 (≠ D205), H210 (≠ A207), E234 (= E231), G259 (= G256), E260 (= E257), Q281 (= Q278), A283 (≠ D280), H310 (= H307), A313 (≠ G310), L332 (≠ Y329), E335 (= E332)
- binding magnesium ion: E234 (= E231), E260 (= E257)
- binding alpha-D-ribofuranose: R85 (≠ N82), P86 (= P83), P239 (= P236), A266 (≠ Y263), E267 (≠ G264)
5olcC Crystal structure of the 3,6-anhydro-d-galactonate cycloisomerase from zobellia galactanivorans (see paper)
31% identity, 96% coverage: 2:370/386 of query aligns to 2:338/351 of 5olcC
- active site: K148 (= K155), K150 (≠ Y157), D178 (= D205), N180 (≠ A207), E204 (= E231), G229 (= G256), E230 (= E257), D253 (= D280), H280 (= H307), E304 (≠ Y329), E309 (≠ P341)
- binding magnesium ion: D178 (= D205), E204 (= E231), E230 (= E257)
2qq6B Crystal structure of mandelate racemase/muconate lactonizing enzyme- like protein from rubrobacter xylanophilus dsm 9941
29% identity, 98% coverage: 2:380/386 of query aligns to 4:386/396 of 2qq6B
- active site: P37 (≠ R43), G79 (≠ N82), D124 (vs. gap), K166 (= K155), D168 (≠ Y157), D213 (= D205), H215 (≠ A207), E239 (= E231), G264 (= G256), E265 (= E257), M286 (≠ Q278), D288 (= D280), H315 (= H307), N316 (≠ V308), E340 (= E332), D345 (vs. gap)
- binding magnesium ion: D213 (= D205), E239 (= E231), E265 (= E257), H315 (= H307)
4hpnA Crystal structure of a proposed galactarolactone cycloisomerase from agrobacterium tumefaciens, target efi-500704, with bound ca, ordered loops (see paper)
29% identity, 93% coverage: 19:377/386 of query aligns to 32:371/378 of 4hpnA
- active site: G50 (= G40), R53 (= R43), T134 (≠ N127), K164 (≠ R161), K166 (≠ Q163), D194 (= D205), N196 (≠ A207), E220 (= E231), G245 (= G256), E246 (= E257), T247 (≠ R258), Q267 (= Q278), D269 (= D280), H296 (= H307), V297 (= V308), W298 (≠ C309), R320 (vs. gap), E329 (≠ F328), F330 (≠ Y329), H334 (= H333)
- binding calcium ion: D194 (= D205), D209 (≠ H220), E220 (= E231), G237 (≠ K248), E246 (= E257)
Sites not aligning to the query:
3bjsB Crystal structure of a member of enolase superfamily from polaromonas sp. Js666
28% identity, 94% coverage: 19:380/386 of query aligns to 31:366/376 of 3bjsB
- active site: P49 (≠ T37), S52 (≠ T44), L93 (≠ P83), G136 (≠ N127), K164 (= K155), R166 (= R161), D194 (= D205), N196 (≠ A207), E220 (= E231), G246 (= G256), E247 (= E257), N248 (≠ R258), Q268 (= Q278), D270 (= D280), H297 (= H307), S298 (≠ V308), S299 (≠ C309), E322 (= E332), C324 (vs. gap), K327 (≠ H333)
- binding magnesium ion: D194 (= D205), E220 (= E231), E247 (= E257)
Sites not aligning to the query:
2qgyA Crystal structure of an enolase from the environmental genome shotgun sequencing of the sargasso sea
28% identity, 94% coverage: 17:379/386 of query aligns to 31:367/376 of 2qgyA
4e5tA Crystal structure of a putative mandelate racemase/muconate lactonizing enzyme (target psi-200750) from labrenzia alexandrii dfl- 11
31% identity, 93% coverage: 19:378/386 of query aligns to 24:372/392 of 4e5tA
A6M2W4 D-galactonate dehydratase family member Cbei_4837 from Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum) (see paper)
24% identity, 99% coverage: 3:386/386 of query aligns to 6:390/399 of A6M2W4
- D205 (= D205) binding
- E231 (= E231) binding
- E257 (= E257) binding
3stpA Crystal structure of a putative galactonate dehydratase
28% identity, 92% coverage: 19:374/386 of query aligns to 48:380/390 of 3stpA
- active site: L66 (≠ T37), S69 (≠ G40), S151 (≠ N127), K177 (= K155), R179 (= R174), P189 (≠ D180), E214 (≠ D205), Y216 (≠ A207), E240 (= E231), G265 (= G256), E266 (= E257), H267 (≠ R258), Q287 (= Q278), D289 (= D280), I311 (≠ V302), H316 (= H307), E336 (= E332), F341 (≠ W337)
- binding magnesium ion: E214 (≠ D205), E240 (= E231), E266 (= E257)
3fxgA Crystal structure of rhamnonate dehydratase from gibberella zeae complexed with mg
31% identity, 89% coverage: 33:375/386 of query aligns to 75:391/407 of 3fxgA
- active site: K184 (= K155), P186 (= P158), E196 (≠ D180), D221 (= D205), Y223 (≠ A207), E247 (= E231), G273 (= G256), E274 (= E257), Q295 (= Q278), D297 (= D280), H324 (= H307), E344 (= E332)
- binding magnesium ion: D221 (= D205), E247 (= E231), E274 (= E257)
3ekgA Crystal structure of l-rhamnonate dehydratase from azotobacter vinelandii complexed with mg and l-tartrate
29% identity, 88% coverage: 33:373/386 of query aligns to 69:384/395 of 3ekgA
- active site: K178 (= K155), P180 (= P158), G184 (≠ I162), S186 (≠ P164), E190 (≠ D180), D215 (= D205), W217 (≠ A207), E241 (= E231), T266 (≠ A254), G268 (= G256), E269 (= E257), Q290 (= Q278), D292 (= D280), H319 (= H307), E339 (vs. gap), D348 (≠ S322)
- binding magnesium ion: D215 (= D205), E241 (= E231), E269 (= E257)
3v3wA Crystal structure of an enolase from the soil bacterium cellvibrio japonicus (target efi-502161) with bound mg and glycerol
25% identity, 98% coverage: 1:380/386 of query aligns to 1:377/397 of 3v3wA
- active site: L36 (≠ A38), R39 (≠ C41), H122 (vs. gap), K144 (= K152), R147 (vs. gap), Q149 (vs. gap), Y159 (= Y157), E179 (≠ D178), D205 (= D205), H207 (≠ A207), E231 (= E231), G256 (= G256), E257 (= E257), V258 (≠ R258), R278 (≠ Q278), T280 (≠ D280), F306 (≠ P306), H307 (= H307), G308 (≠ V308), A309 (≠ C309), E334 (= E332)
- binding magnesium ion: D205 (= D205), E231 (= E231), E257 (= E257)
Sites not aligning to the query:
2qjmA Crystal structure of the k271e mutant of mannonate dehydratase from novosphingobium aromaticivorans complexed with mg and d-mannonate (see paper)
26% identity, 98% coverage: 1:380/386 of query aligns to 1:364/384 of 2qjmA
- active site: G121 (vs. gap), R147 (≠ K155), Q149 (≠ R161), D192 (= D205), H194 (≠ A207), E218 (= E231), G243 (= G256), E244 (= E257), R265 (≠ Q278), T267 (≠ D280), H294 (= H307), E321 (= E332)
- binding d-mannonic acid: N37 (≠ Y39), R147 (≠ K155), D192 (= D205), H194 (≠ A207), E244 (= E257), H294 (= H307), A296 (≠ C309), D298 (vs. gap), E321 (= E332)
- binding magnesium ion: D192 (= D205), E218 (= E231), E244 (= E257)
Sites not aligning to the query:
B3PDB1 D-galactonate dehydratase family member RspA; D-mannonate dehydratase; Starvation sensing protein RspA homolog; EC 4.2.1.-; EC 4.2.1.8 from Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) (see paper)
25% identity, 98% coverage: 1:380/386 of query aligns to 1:382/402 of B3PDB1
- D210 (= D205) binding
- E236 (= E231) binding
- E262 (= E257) binding
3sqsA Crystal structure of a putative mandelate racemase/muconate lactonizing protein from dinoroseobacter shibae dfl 12
28% identity, 93% coverage: 19:378/386 of query aligns to 44:380/386 of 3sqsA
- active site: L62 (≠ T37), N65 (≠ G40), S147 (≠ N127), K173 (= K155), R175 (vs. gap), G177 (≠ P158), G179 (≠ A160), K181 (≠ I162), A185 (≠ G166), E210 (≠ D205), Y212 (≠ A207), E236 (= E231), G261 (= G256), E262 (= E257), H263 (≠ R258), Q283 (= Q278), D285 (= D280), I307 (≠ V302), H312 (= H307), G314 (≠ A314), E332 (= E332), F337 (≠ W337)
- binding magnesium ion: E210 (≠ D205), E236 (= E231), E262 (= E257), Y347 (vs. gap), F350 (vs. gap), D351 (vs. gap)
Query Sequence
>AZOBR_RS04900 FitnessBrowser__azobra:AZOBR_RS04900
MKITKATIYVINSEGLKPVIVKLDTDEGISGLGEVATAYGCGRTGAAGMIQDLCARFVIG
ADPTRINSLVGEMYDQSFWLKNPGGIAGAGLSAIEQALWDIRARALNVPVHELFGGRMRD
TLAYYANGWYFGATSVADLLRQAEAAVKDGHKALKMYPLARIQPNGTLRHTVNRYADDRD
AVNAALDIVAQVRRTVGADVTLMLDLAGGFSVSDTIRFVHGLEDLDIAFIEEICDPGDLG
ALEQVAAKTNIPIATGERQYLRYGFRDLLERRAVGILQPDIGNTGGFAETHKIAAMADAY
GVKVQPHVCGSSVAASIATHLSAVIPNFYVQEHFPYWARIPGWIEVATEPFESRLKNGAL
QIPDGPGYGVTLKDAVVREHIWAEVR
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory