SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AZOBR_RS05825 FitnessBrowser__azobra:AZOBR_RS05825 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7bw9A Crystal structure of serine acetyltransferase isoform 3 in complex with cysteine from entamoeba histolytica
45% identity, 58% coverage: 15:173/276 of query aligns to 90:252/280 of 7bw9A

query
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7bw9A

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binding cysteine: D114 (= D35), P115 (= P36), D179 (= D100), H180 (= H101), A206 (≠ G127), R214 (= R135), H215 (= H136)

8i04A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with serine (see paper)
41% identity, 58% coverage: 27:187/276 of query aligns to 80:240/258 of 8i04A

query
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8i04A

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binding serine: D88 (= D35), P89 (= P36), D153 (= D100), H154 (= H101), G180 (= G127), R188 (= R135), H189 (= H136)

8i09A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with butyl gallate (see paper)
41% identity, 58% coverage: 27:187/276 of query aligns to 83:243/246 of 8i09A

query
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binding cysteine: D91 (= D35), D156 (= D100), H157 (= H101), G164 (= G108), R191 (= R135), H192 (= H136)
binding butyl 3,4,5-tris(oxidanyl)benzoate: G183 (= G127), T184 (= T128), G202 (= G146), K218 (≠ R162), I219 (≠ V163), G220 (= G164), L226 (≠ V170), A236 (≠ V180)

8i06A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with coa (see paper)
41% identity, 58% coverage: 27:187/276 of query aligns to 84:244/244 of 8i06A

query
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8i06A

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binding coenzyme a: H158 (= H101), L177 (≠ Y120), Q178 (≠ H121), G203 (= G146), A204 (= A147), G221 (= G164), A222 (= A165), T235 (≠ A178), A237 (≠ V180)
binding cysteine: P93 (= P36), H158 (= H101)

6wyeA Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) (see paper)
40% identity, 61% coverage: 20:187/276 of query aligns to 78:245/261 of 6wyeA

query
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6wyeA

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binding (2S)-2-hydroxybutanedioic acid: D93 (= D35), P94 (= P36), D158 (= D100), H159 (= H101), G185 (= G127), R193 (= R135), H194 (= H136)

4hzdA Crystal structure of serine acetyltransferase in complex with coenzyme a from brucella abortus strain s19 (see paper)
39% identity, 61% coverage: 19:187/276 of query aligns to 75:244/250 of 4hzdA

query
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4hzdA

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binding coenzyme a: H158 (= H101), G203 (= G146), A204 (= A147), A221 (≠ G164), A222 (= A165), T235 (≠ A178), A237 (≠ V180), I244 (≠ V187)
binding magnesium ion: S224 (≠ A167), A237 (≠ V180), G238 (= G181)

1t3dA Crystal structure of serine acetyltransferase from e.Coli at 2.2a (see paper)
40% identity, 58% coverage: 27:187/276 of query aligns to 84:244/262 of 1t3dA

query
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1t3dA

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binding cysteine: D92 (= D35), P93 (= P36), D157 (= D100), G184 (= G127), R192 (= R135), H193 (= H136)

7ra4A Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) in complex with serine (see paper)
40% identity, 61% coverage: 20:187/276 of query aligns to 76:243/243 of 7ra4A

query
sites
7ra4A

V

I

F

S

K

K

H

C

L

V

R

E

E

A

E

D

I

L

D

K

G

A

I

I

M

Y

A

E

R

R

D
|
D

P
|
P

A

A

A

C

R

D

S

E

R

Y

A

S

E

L

V

P

A

L

L

L

C

Y

Y

F

P

K

G

G

F

F

H

H

A

A

I

I

V

Q

L

A

H

H

R

R

V

I

A

N

R

H

Y

R

C

L

W

Y

D

L

R

D

R

G

W

R

H

K

L

T

M

L

A

A

R

Y

A

F

V

L

S

Q

Q

N

I

R

A

M

R

S

A

E

L

V

T

F

G

G

I

V

E

D

I

I

H

H

P

P

G

A

A

A

T

R

I

L

G

G

R

Y

R

G

F

L

F

M

I

L

D
|
D

H
|
H

G

A

M

T

G

G

V

F

V

V

I

A

G

G

E

E

T

T

A

A

E

V

I

L

G

G

D

N

D

N

V

I

M

S

L

I

Y

L

H

H

G

G

V

V

T

T

L

L

G

G

G

G

T

S

S

G

L

K

N

E

Q

G

G

G

K

D

R
|
R

H
|
H

P

P

T

K

L

I

E

G

S

D

G

G

V

V

I

M

V

I

G

G

A

A

G

N

A

A

K

S

V

I

L

L

G

G

A

N

I

I

T

R

I

I

G

G

R

S

G

N

A

A

R

K

V

I

G

G

A

A

N

G

A

S

V

V

V

V

V

V

A

S

D

D

V

V

A

P

P

P

G

S

I

I

A

T

V

V

V

V

G

G

I

V

P

P

A

A

K

K

A

P

V

V

binding serine: D91 (= D35), P92 (= P36), D156 (= D100), H157 (= H101), R191 (= R135), H192 (= H136)

1ssqD Serine acetyltransferase- complex with cysteine (see paper)
40% identity, 62% coverage: 27:198/276 of query aligns to 80:250/257 of 1ssqD

query
sites
1ssqD

E

D

I

I

D

Q

G

A

I

V

M

R

A

H

R

R

D
|
D

P

P

A

A

A

V

R

E

S

L

R

W

A

S

E

T

V

P

A

L

L

L

C

Y

Y

L

P

K

G

G

F

F

H

H

A

A

I

I

V

Q

L

S

H

Y

R

R

V

I

A

T

R

H

Y

Y

C

L

W

W

D

N

R

Q

-

N

R

R

W

K

H

S

L

L

M

A

A

L

R

Y

A

L

V

Q

S

N

Q

Q

I

I

A

S

R

V

A

A

L

F

T

D

G

-

I

V

E

D

I

I

H

H

P

P

G

A

A

A

T

K

I

I

G

G

R

H

R

G

F

I

F

M

I

F

D
|
D

H
|
H

G

A

M

T

G

G

V

I

V

V

I

V

G

G

E

E

T

T

A

S

E

V

I

I

G

E

D

N

D

D

V

V

M

S

L

I

Y

L

H

Q

G

G

V

V

T

T

L

L

G

G

G

G

T

T

S

G

L

K

N

E

Q

S

G

G

K

D

R

R

H

H

P

P

T

K

L

V

E

R

S

E

G

G

V

V

I

M

V

I

G

G

A

A

G

G

A

A

K

K

V

I

L

L

G

G

A

N

I

I

T

E

I

V

G

G

R

K

G

Y

A

A

R

K

V

I

G

G

A

A

N

N

A

S

V

V

V

V

V

L

A

N

D

P

V

V

A

P

P

E

G

Y

I

A

A

T

V

A

V

A

G

G

I

V

P

P

A

A

K

R

A

-

V

I

V

V

T

S

R

Q

D

D

R

K

V

A

E

A

T

K

Q

P

K

A

F

F

binding cysteine: D88 (= D35), D153 (= D100), H154 (= H101)

4n69A Soybean serine acetyltransferase complexed with serine (see paper)
47% identity, 56% coverage: 34:187/276 of query aligns to 90:243/243 of 4n69A

query
sites
4n69A

R

R

D

D

P

P

A

A

A

C

R

V

S

S

R

Y

A

S

E

H

V

C

A

L

L

L

C

N

Y

Y

P

K

G

G

F

F

H

L

A

A

I

C

V

Q

L

A

H

H

R

R

V

V

A

A

R

H

Y

L

C

L

W

W

D

R

R

Q

R

S

W

R

H

R

L

P

M

L

A

A

R

L

A

A

V

L

S

H

Q

S

I

R

A

I

R

A

A

N

L

V

T

F

G

A

I

V

E

D

I

I

H

H

P

P

G

A

A

A

T

R

I

I

G

G

R

K

R

G

F

I

F

L

I

F

D

D

H

H

G

A

M

T

G

G

V

V

V

V

I

V

G

G

E

E

T

T

A

A

E

V

I

I

G

G

D

N

D

N

V

V

M

S

L

I

Y

L

H

H

G

H

V

V

T

T

L

L

G

G

G
|
G

T

T

S

G

L

K

N

V

Q

G

G

G

K

D

R
|
R

H
|
H

P

P

T

K

L

I

E

G

S

D

G

G

V

V

I

L

V

I

G

G

A

A

G

G

A

A

K

T

V

I

L

L

G

G

A

N

I

I

T

K

I

I

G

G

R

E

G

G

A

A

R

K

V

V

G

G

A

A

N

G

A

S

V

V

V

V

V

L

A

I

D

D

V

V

A

P

P

P

G

R

I

T

A

T

V

A

V

V

G

G

I

N

P

P

A

A

K

R

A

L

V

V

binding serine: G183 (= G127), R191 (= R135), H192 (= H136)

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
40% identity, 58% coverage: 27:187/276 of query aligns to 87:247/272 of 3gvdI

query
sites
3gvdI

E

D

I

I

D

L

G

A

I

V

M

R

A

L

R

R

D
|
D

P
|
P

A

A

A

V

R

D

S

K

R

Y

A

S

E

T

V

P

A

L

L

L

C

Y

Y

L

P

K

G

G

F

F

H

H

A

A

I

L

V

Q

L

A

H

Y

R

R

V

I

A

G

R

H

Y

W

C

L

W

W

D

A

R

Q

R

D

W

R

H

K

L

A

M

L

A

A

R

I

A

Y

V

L

S

Q

Q

N

I

Q

A

V

R

S

A

V

L

A

T

F

G

G

I

V

E

D

I

I

H

H

P

P

G

A

A

A

T

T

I

I

G

G

R

C

R

G

F

I

F

M

I

L

D
|
D

H

H

G

A

M

T

G

G

V

I

V

V

I

I

G

G

E

E

T

T

A

A

E

V

I

V

G

E

D

N

D

D

V

V

M

S

L

I

Y

L

H

Q

G

S

V

V

T

T

L

L

G
|
G

G
|
G

T

T

S

G

L

K

N

T

Q

S

G

G

K

D

R
|
R

H

H

P

P

T

K

L

I

E

R

S

E

G

G

V

V

I

M

V

I

G

G

A

A

G

G

A

A

K

K

V

I

L

L

G

G

A

N

I

I

T

E

I

V

G

G

R

R

G

G

A

A

R

K

V

I

G

G

A

A

N

G

A

S

V

V

V

V

V

L

A

Q

D

S

V

V

A

P

P

A

G

H

I

T

A

T

V

A

V

A

G

G

I

V

P

P

A

A

K

R

A

I

V

V

binding cysteine: D95 (= D35), P96 (= P36), D160 (= D100), G186 (= G126), G187 (= G127), R195 (= R135)

4h7oA Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
43% identity, 58% coverage: 27:187/276 of query aligns to 80:240/258 of 4h7oA

query
sites
4h7oA

E

D

I

I

D

C

G

A

I

T

M

V

A

N

R

R

D
|
D

P
|
P

A

A

A

V

R

S

S

M

R

Y

A

S

E

M

V

P

A

L

L

L

C

Y

Y

L

P

K

G

G

F

Y

H

H

A

A

I

L

V

Q

L

G

H

Y

R

R

V

V

A

A

R

N

Y

W

C

L

W

W

D

R

R

Q

-

G

R

R

W

K

H

A

L

L

M

A

A

T

R
x
Y

A

F

V

Q

S

N

Q
|
Q

I

I

A

S

R

V

A

A

L

C

T

Q

G

-

I

V

E

D

I

I

H

H

P

P

G

A

A

A

T

R

I

I

G

G

R

R

R

G

F

I

F

M

I

L

D
|
D

H
|
H

G

A

M

T

G

G

V

I

V

V

I

I

G

G

E

E

T

T

A

A

E

V

I

V

G

E

D

D

D

D

V

V

M

S

L

I

Y

L

H

Q

G

D

V

V

T

T

L

L

G

G

G
|
G

T

T

S

G

L

K

N

E

Q

C

G

G

K

D

R
|
R

H
|
H

P

P

T

K

L

I

E

R

S

E

G

G

V

V

I

M

V

I

G

G

A

A

G

G

A

A

K

K

V

I

L

L

G

G

A

N

I

I

T

E

I

V

G

G

R

E

G

G

A

A

R

K

V

I

G

G

A

S

N

G

A

S

V

V

V

V

V

L

A

Q

D

A

V

V

A

P

P

P

G

H

I

T

A

T

V

V

V

A

G

G

I

V

P

P

A

A

K

R

A

I

V

V

binding arginine: Y127 (≠ R73), Q131 (= Q77)
binding cysteine: D88 (= D35), P89 (= P36), D153 (= D100), H154 (= H101), G180 (= G127), R188 (= R135), H189 (= H136)

Sites not aligning to the query:

binding arginine: 51, 53

3q1xA Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-serine (see paper)
41% identity, 54% coverage: 24:173/276 of query aligns to 103:260/267 of 3q1xA

query
sites
3q1xA

L

L

R

K

E

T

E

D

I

L

D

I

G

A

I

A

M

Y

A

A

R

G

D

D

P

P

A

A

A

A

R

P

S

G

R

L

A

S

E

L

V

I

A

I

L

R

C

C

Y

Y

P

P

G

G

F

F

H

Q

A

A

I

V

V

I

L

V

H

Y

R

R

V

I

A

A

R

H

Y

V

C

L

W

Y

D

E

R

C

R

G

W

E

H

R

L

Y

M

Y

A

C

R

R

A

E

V

M

S

M

Q

E

I

S

A

V

R

H

A

S

L

Y

T

T

G

S

I

I

E

D

I

I

H

H

P

P

G

G

A

A

T

S

I

I

G

K

R

G

R

H

F

F

F

F

I

I

D

D

H

H

G

G

M

V

G

G

V

V

V

V

I

I

G

G

E

E

T

T

A

A

E

I

I

I

G

G

D

E

D

W

V

C

M

R

L

I

Y

Y

H

Q

G

S

V

V

T

T

L

L

G

G

G

A

T

M

S
x
H

L

F

N

Q

Q

E

-

E

-

G

-

G

-

V

-

I

-

K

-

R

-

G

G

T

K

K

R
|
R

H
|
H

P

P

T

T

L

V

E

G

S

D

G

Y

V

V

I

T

V

I

G

G

A

T

G

G

A

A

K

K

V

V

L

L

G

G

A

N

I

I

T

I

I

V

G

G

R

S

G

H

A

V

R

R

V

I

G

G

A

A

N

N

A

C

V

W

V

I

V

D

A

R

D

D

V

V

binding serine: H208 (≠ S129), R222 (= R135), H223 (= H136)

3p47A Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-cysteine (see paper)
41% identity, 54% coverage: 24:173/276 of query aligns to 105:262/270 of 3p47A

query
sites
3p47A

L

L

R

K

E

T

E

D

I

L

D

I

G

A

I

A

M

Y

A

A

R

G

D

D

P

P

A

A

A

A

R

P

S

G

R

L

A

S

E

L

V

I

A

I

L

R

C

C

Y

Y

P

P

G

G

F

F

H

Q

A

A

I

V

V

I

L

V

H

Y

R

R

V

I

A

A

R

H

Y

V

C

L

W

Y

D

E

R

C

R

G

W

E

H

R

L

Y

M

Y

A

C

R

R

A

E

V

M

S

M

Q

E

I

S

A

V

R

H

A

S

L

Y

T

T

G

S

I

I

E

D

I

I

H

H

P

P

G

G

A

A

T

S

I

I

G

K

R

G

R

H

F

F

F

F

I

I

D

D

H

H

G

G

M

V

G

G

V

V

V

V

I

I

G

G

E

E

T

T

A

A

E

I

I

I

G

G

D

E

D

W

V

C

M

R

L

I

Y

Y

H

Q

G

S

V

V

T

T

L

L

G

G

G
x
A

T

M

S
x
H

L

F

N

Q

Q

E

-

E

-

G

-

G

-

V

-

I

-

K

-

R

-

G

G

T

K

K

R
|
R

H
|
H

P

P

T

T

L

V

E

G

S

D

G

Y

V

V

I

T

V

I

G

G

A

T

G

G

A

A

K

K

V

V

L

L

G

G

A

N

I

I

T

I

I

V

G

G

R

S

G

H

A

V

R

R

V

I

G

G

A

A

N

N

A

C

V

W

V

I

V

D

A

R

D

D

V

V

binding cysteine: A208 (≠ G127), H210 (≠ S129), R224 (= R135), H225 (= H136)

4n6bA Soybean serine acetyltransferase complexed with coa (see paper)
46% identity, 54% coverage: 40:187/276 of query aligns to 89:233/233 of 4n6bA

query
sites
4n6bA

S

S

R

Y

A

S

E

H

V

C

A

L

L

L

C

N

Y

Y

P

K

G

G

F

F

H

L

A

A

I

C

V

Q

L

A

H

H

R

R

V

V

A

A

R

H

Y

L

C

L

W

W

D

R

R

Q

R

S

W

R

H

R

L

P

M

L

A

A

R

L

A

A

V

L

S

H

Q

S

I

R

A

I

R

A

A

N

L

V

T

F

G

A

I

V

E

D

I

I

H

H

P

P

G

A

A

A

T

R

I

I

G

G

R

K

R

G

F

I

F

L

I

F

D

D

H
|
H

G

A

M

T

G

G

V

V

V

V

I

V

G

G

E

E

T

T

A

A

E

V

I

I

G

G

D

N

D

N

V

V

M

S

L

I

Y
x
L

H

H

G

H

V

V

T

T

L

L

G

G

G
|
G

T

T

S

G

L

-

N

-

Q

-

G

G

K

D

R

R

H

H

P

P

T

K

L

I

E

G

S

D

G

G

V

V

I

L

V

I

G

G

A

A

G

G

A

A

K

T

V

I

L

L

G

G

A

N

I

I

T

K

I

I

G

G

R

E

G

G

A

A

R
x
K

V

V

G
|
G

A
|
A

N

G

A

S

V

V

V

V

V
x
L

A

I

D

D

V

V

A

P

P

P

G

R

I

T

A

T

V

A

V
|
V

G

G

I

N

P

P

A

A

K

R

A

L

V

V

binding coenzyme a: H150 (= H101), L169 (≠ Y120), G176 (= G127), K208 (≠ R162), G210 (= G164), A211 (= A165), L216 (≠ V170), V226 (= V180)

1sstA Serine acetyltransferase- complex with coa (see paper)
41% identity, 58% coverage: 27:187/276 of query aligns to 80:233/233 of 1sstA

query
sites
1sstA

E

D

I

I

D

Q

G

A

I

V

M

R

A

H

R

R

D

D

P

P

A

A

A

V

R

E

S

L

R

W

A

S

E

T

V

P

A

L

L

L

C

Y

Y

L

P

K

G

G

F

F

H

H

A

A

I

I

V

Q

L

S

H

Y

R

R

V

I

A

T

R

H

Y

Y

C

L

W

W

D

N

R

Q

-

N

R

R

W

K

H

S

L

L

M

A

A

L

R

Y

A

L

V

Q

S

N

Q

Q

I

I

A

S

R

V

A

A

L

F

T

-

G

D

I

V

E

D

I

I

H

H

P

P

G

A

A

A

T

K

I

I

G

G

R

H

R

G

F

I

F

M

I

F

D

D

H
|
H

G

A

M

T

G

G

V

I

V

V

I

V

G

G

E

E

T

T

A

S

E

V

I

I

G

E

D

N

D

D

V

V

M

S

L

I

Y
x
L

H

Q

G

G

V

V

T

T

L

L

G

G

G
|
G

T
|
T

S

-

L

-

N

-

Q

-

G

-

K

-

R

-

H

H

P

P

T

K

L

V

E

R

S

E

G

G

V

V

I

M

V

I

G

G

A
|
A

G

G

A

A

K

K

V

I

L
|
L

G

G

A

N

I

I

T

E

I

V

G

G

R

K

G

Y

A

A

R
x
K

V

I

G
|
G

A
|
A

N

N

A

S

V
|
V

V

V

V
x
L

A

N

D

P

V

V

A

P

P

E

G

Y

I

A

A
x
T

V

A

V
x
A

G

G

I
x
V

P
|
P

A

A

K

R

A

I

V
|
V

binding coenzyme a: H154 (= H101), L173 (≠ Y120), G180 (= G127), T181 (= T128), A193 (= A147), L198 (= L152), K208 (≠ R162), G210 (= G164), A211 (= A165), V214 (= V168), L216 (≠ V170), T224 (≠ A178), A226 (≠ V180), V228 (≠ I182), P229 (= P183), V233 (= V187)

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
27% identity, 39% coverage: 93:201/276 of query aligns to 77:196/201 of 1krvA

query
sites
1krvA

I

I

G

G

R

R

R

N

F

F

F
x
Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V
|
V

I

D

G
x
D

E

Y

T

T

A

V

E

T

I

I

G

G

D

D

D

N

V

V

M

L

L

I

Y
x
A

H

P

G

N

V

V

T

T

L

L

G
x
S

G

V

T
|
T

S

G

L

H

-

P

-

V

-

H

-

H

-

E

-

L

-

R

N

K

Q

N

G

G

K

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

E

G

S

N

G

N

V

V

I
x
W

V

I

G
|
G

A
x
S

G

H

A

V

K

V

V

I

L
x
N

G

P

A

G

I

V

T

T

I

I

G

G

R

D

G

N

A

S

R

V

V

I

G

G

A
|
A

N

G

A

S

V

I

V

V

V

T

A

K

D

D

V

I

A

P

P

P

G

N

I

V

A

V

V

A

V

A

G

G

I

V

P
|
P

A

C

K
x
R

A

V

V

I

-
x
R

V

E

T

I

R

N

D

D

R

R

V

D

E

K

T

H

Q

Y

K

Y

F

F

M

K

P

D

Y

Y

binding 4-nitrophenyl beta-D-galactopyranoside: Y82 (≠ F98), N84 (≠ D100), V90 (= V106), D92 (≠ G108), M126 (≠ R135)
binding coenzyme a: A104 (≠ Y120), S110 (≠ G126), T112 (= T128), W138 (≠ I144), G140 (= G146), S141 (≠ A147), N146 (≠ L152), A159 (= A165), P177 (= P183), R179 (≠ K185), R182 (vs. gap)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25, 70

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
27% identity, 39% coverage: 93:201/276 of query aligns to 77:196/201 of 1kruA

query
sites
1kruA

I

I

G

G

R

R

R

N

F

F

F
x
Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V
|
V

I

D

G
x
D

E

Y

T

T

A

V

E

T

I

I

G

G

D

D

D

N

V

V

M
x
L

L

I

Y

A

H

P

G

N

V

V

T

T

L

L

G

S

G

V

T

T

S

G

L
x
H

-

P

-

V

-

H

-

H

-

E

-

L

-

R

N

K

Q

N

G

G

K

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

E

G

S

N

G

N

V

V

I
x
W

V

I

G
|
G

A
x
S

G

H

A

V

K

V

V

I

L

N

G

P

A

G

I

V

T

T

I

I

G

G

R

D

G

N

A

S

R

V

V

I

G
|
G

A
|
A

N

G

A

S

V

I

V

V

V

T

A

K

D

D

V

I

A

P

P

P

G

N

I

V

A

V

V

A

V

A

G

G

I

V

P
|
P

A

C

K

R

A

V

V

I

-
x
R

V

E

T

I

R

N

D

D

R

R

V

D

E

K

T

H

Q

Y

K

Y

F

F

M

K

P

D

Y

Y

binding coenzyme a: H114 (≠ L130), W138 (≠ I144), G140 (= G146), S141 (≠ A147), G158 (= G164), A159 (= A165), P177 (= P183), R182 (vs. gap)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: Y82 (≠ F98), Y82 (≠ F98), N84 (≠ D100), V90 (= V106), D92 (≠ G108), L102 (≠ M118), H114 (≠ L130), M126 (≠ R135)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25, 62, 70

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
27% identity, 39% coverage: 93:201/276 of query aligns to 77:196/200 of 1krrA

query
sites
1krrA

I

I

G

G

R

R

R

N

F

F

F

Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V

V

I

D

G

D

E

Y

T

T

A

V

E

T

I

I

G

G

D

D

D

N

V

V

M

L

L
x
I

Y
x
A

H
x
P

G

N

V

V

T

T

L

L

G

S

G

V

T
|
T

S

G

L
x
H

-

P

-

V

-

H

-

H

-

E

-

L

-

R

N

K

Q

N

G

G

K

E

R

M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

E

G

S

N

G

N

V

V

I

W

V

I

G
|
G

A
x
S

G

H

A

V

K

V

V

I

L
x
N

G

P

A

G

I

V

T

T

I

I

G

G

R

D

G

N

A

S

R

V

V

I

G
|
G

A
|
A

N

G

A

S

V

I

V

V

V

T

A

K

D

D

V

I

A

P

P

P

G

N

I

V

A

V

V

A

V
x
A

G

G

I

V

P
|
P

A

C

K

R

A

V

V

I

-
x
R

V

E

T

I

R

N

D

D

R

R

V

D

E

K

T

H

Q

Y

K

Y

F

F

M

K

P

D

Y

Y

binding acetyl coenzyme *a: N84 (≠ D100), I103 (≠ L119), A104 (≠ Y120), P105 (≠ H121), T112 (= T128), H114 (≠ L130), G140 (= G146), S141 (≠ A147), N146 (≠ L152), G158 (= G164), A159 (= A165), A174 (≠ V180), P177 (= P183), R182 (vs. gap)

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
27% identity, 39% coverage: 93:201/276 of query aligns to 78:197/203 of P07464

query
sites
P07464

I

I

G

G

R

R

R

N

F

F

F

Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V

V

I

D

G
x
D

E

Y

T

T

A

V

E

T

I

I

G

G

D

D

D

N

V

V

M

L

L

I

Y

A

H

P

G

N

V

V

T

T

L

L

G

S

G

V

T

T

S

G

L
x
H

-

P

-

V

-

H

-

H

-

E

-

L

-

R

N

K

Q

N

G

G

K

E

R

M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

E

G

S

N

G

N

V

V

I

W

V

I

G

G

A
x
S

G

H

A

V

K

V

V

I

L

N

G

P

A

G

I

V

T

T

I

I

G

G

R

D

G

N

A

S

R

V

V

I

G

G

A
|
A

N

G

A

S

V

I

V

V

V
x
T

A
x
K

D

D

V

I

A

P

P

P

G

N

I

V

A

V

V

A

V

A

G

G

I

V

P

P

A

C

K
x
R

A

V

V

I

-
x
R

V

E

T

I

R

N

D

D

R

R

V

D

E

K

T

H

Q

Y

K

Y

F

F

M

K

P

D

Y

Y

N85 (≠ D100) binding in other chain; binding in other chain
D93 (≠ G108) binding
H115 (≠ L130) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A147) binding in other chain
A160 (= A165) binding in other chain
TK 165:166 (≠ VA 170:171) binding
R180 (≠ K185) binding
R183 (vs. gap) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding
71 binding

Query Sequence

>AZOBR_RS05825 FitnessBrowser__azobra:AZOBR_RS05825
LRKITSSGKRADYTTAGVPVFKHLREEIDGIMARDPAARSRAEVALCYPGFHAIVLHRVA
RYCWDRRWHLMARAVSQIARALTGIEIHPGATIGRRFFIDHGMGVVIGETAEIGDDVMLY
HGVTLGGTSLNQGKRHPTLESGVIVGAGAKVLGAITIGRGARVGANAVVVADVAPGIAVV
GIPAKAVVTRDRVETQKFMPYGTPCGDIPDPVARALNGLLDQVSTLRRRVEELESERGDA
HNSSVYETSGVPNGSARPDNGVRQPVSVDGGPAASC

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory