SitesBLAST
Comparing AZOBR_RS06155 FitnessBrowser__azobra:AZOBR_RS06155 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1mtoA Crystal structure of a phosphofructokinase mutant from bacillus stearothermophilus bound with fructose-6-phosphate (see paper)
40% identity, 94% coverage: 5:343/361 of query aligns to 2:307/319 of 1mtoA
- active site: G11 (= G14), R72 (≠ N79), C73 (≠ R80), D103 (= D118), G104 (= G119), G124 (≠ K140), T125 (= T141), D127 (= D143), D129 (= D145), R171 (= R187)
- binding 6-O-phosphono-beta-D-fructofuranose: R72 (≠ N79), D127 (= D143), R162 (= R178), M169 (= M185), G170 (= G186), R171 (= R187), E222 (= E238), R243 (= R279), H249 (= H285), R252 (= R288)
6pfkA Phosphofructokinase, inhibited t-state (see paper)
40% identity, 94% coverage: 5:343/361 of query aligns to 2:307/319 of 6pfkA
- active site: G11 (= G14), R72 (≠ N79), C73 (≠ R80), D103 (= D118), G104 (= G119), G124 (≠ K140), T125 (= T141), D127 (= D143), D129 (= D145), R171 (= R187)
- binding 2-phosphoglycolic acid: R21 (= R24), R25 (≠ H28), G55 (≠ P61), G58 (= G65), D59 (≠ N66), R154 (≠ Q170), R211 (≠ T227), K213 (≠ R229), K214 (≠ N230)
3u39D Crystal structure of the apo bacillus stearothermophilus phosphofructokinase (see paper)
40% identity, 94% coverage: 5:343/361 of query aligns to 2:307/319 of 3u39D
- active site: G11 (= G14), R72 (≠ N79), C73 (≠ R80), D103 (= D118), G104 (= G119), G124 (≠ K140), T125 (= T141), D127 (= D143), D129 (= D145), R171 (= R187)
- binding calcium ion: R63 (≠ M70), G64 (= G71)
P00512 ATP-dependent 6-phosphofructokinase; ATP-PFK; Phosphofructokinase; Phosphohexokinase; EC 2.7.1.11 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see 2 papers)
40% identity, 94% coverage: 5:343/361 of query aligns to 2:307/319 of P00512
- RSVVR 21:25 (≠ RAVVH 24:28) binding
- D59 (≠ N66) binding
- RC 72:73 (≠ NR 79:80) binding
- D103 (= D118) binding
- TID 125:127 (= TID 141:143) binding in other chain
- R154 (≠ Q170) binding in other chain
- R162 (= R178) binding
- MGR 169:171 (= MGR 185:187) binding in other chain
- GAE 185:187 (≠ GAD 201:203) binding in other chain
- R211 (≠ T227) binding in other chain
- KKH 213:215 (≠ RNF 229:231) binding in other chain
- E222 (= E238) binding in other chain
- R243 (= R279) binding
- HVQR 249:252 (= HVQR 285:288) binding in other chain
4pfkA Phosphofructokinase. Structure and control (see paper)
40% identity, 94% coverage: 5:343/361 of query aligns to 2:307/319 of 4pfkA
- active site: G11 (= G14), R72 (≠ N79), C73 (≠ R80), D103 (= D118), G104 (= G119), G124 (≠ K140), T125 (= T141), D127 (= D143), D129 (= D145), R171 (= R187)
- binding adenosine-5'-diphosphate: S9 (= S12), G10 (= G13), Y41 (≠ T45), R72 (≠ N79), C73 (≠ R80), F76 (≠ Y86), K77 (≠ P87), Q82 (≠ T92), G104 (= G119), S105 (= S120), Q107 (≠ A122), G108 (≠ I123), K111 (= K126), R154 (≠ Q170), G185 (= G201), E187 (≠ D203), R211 (≠ T227), G212 (= G228), K213 (≠ R229), K214 (≠ N230), H215 (≠ F231)
- binding 6-O-phosphono-beta-D-fructofuranose: I126 (= I142), D127 (= D143), M169 (= M185), G170 (= G186), R171 (= R187), E222 (= E238), H249 (= H285), R252 (= R288)
- binding magnesium ion: G185 (= G201), E187 (≠ D203)
3pfkA Phosphofructokinase. Structure and control (see paper)
40% identity, 94% coverage: 5:343/361 of query aligns to 2:307/319 of 3pfkA
- active site: G11 (= G14), R72 (≠ N79), C73 (≠ R80), D103 (= D118), G104 (= G119), G124 (≠ K140), T125 (= T141), D127 (= D143), D129 (= D145), R171 (= R187)
- binding phosphate ion: R154 (≠ Q170), R171 (= R187), G185 (= G201), K213 (≠ R229), H249 (= H285), R252 (= R288)
4i4iA Crystal structure of bacillus stearothermophilus phosphofructokinase mutant t156a bound to pep (see paper)
40% identity, 94% coverage: 5:343/361 of query aligns to 2:307/319 of 4i4iA
- active site: G11 (= G14), R72 (≠ N79), C73 (≠ R80), D103 (= D118), G104 (= G119), G124 (≠ K140), T125 (= T141), D127 (= D143), D129 (= D145), R171 (= R187)
- binding phosphoenolpyruvate: R21 (= R24), R25 (≠ H28), G55 (≠ P61), V57 (= V63), G58 (= G65), D59 (≠ N66), R154 (≠ Q170), R211 (≠ T227), K213 (≠ R229), K214 (≠ N230), H215 (≠ F231)
1pfkA Crystal structure of the complex of phosphofructokinase from escherichia coli with its reaction products (see paper)
39% identity, 92% coverage: 5:336/361 of query aligns to 3:301/320 of 1pfkA
- active site: G12 (= G14), R73 (≠ N79), F74 (≠ Y86), D104 (= D118), G105 (= G119), G125 (≠ K140), T126 (= T141), D128 (= D143), D130 (= D145), R172 (= R187)
- binding adenosine-5'-diphosphate: S10 (= S12), G11 (= G13), G12 (= G14), R22 (= R24), R26 (= R29), Y42 (≠ T45), R55 (≠ L60), Y56 (≠ P61), S59 (≠ G65), D60 (≠ N66), R73 (≠ N79), F74 (≠ Y86), P75 (= P87), F77 (≠ D95), R78 (= R96), G103 (= G117), D104 (= D118), G105 (= G119), S106 (= S120), M108 (≠ A122), G109 (≠ I123), R112 (≠ K126), R155 (≠ Q170), G186 (= G201), E188 (≠ D203), G213 (= G228), K214 (≠ R229), K215 (≠ N230), H216 (≠ F231)
- binding 1,6-di-O-phosphono-beta-D-fructofuranose: G12 (= G14), R73 (≠ N79), T126 (= T141), I127 (= I142), D128 (= D143), M170 (= M185), G171 (= G186), R172 (= R187), E223 (= E238), H250 (= H285), R253 (= R288)
- binding magnesium ion: G186 (= G201), E188 (≠ D203)
P0A796 ATP-dependent 6-phosphofructokinase isozyme 1; ATP-PFK 1; Phosphofructokinase 1; 6-phosphofructokinase isozyme I; Phosphohexokinase 1; Sedoheptulose-7-phosphate kinase; EC 2.7.1.11 from Escherichia coli (strain K12) (see 2 papers)
39% identity, 92% coverage: 5:336/361 of query aligns to 3:301/320 of P0A796
- G12 (= G14) binding
- RGVV-R 22:26 (≠ RAVVHR 24:29) binding
- RYSV-SD 55:60 (≠ LPQVDGN 60:66) binding
- R------F 73:74 (≠ NRGDPFAY 79:86) binding
- GDGS 103:106 (= GDGS 117:120) binding
- D104 (= D118) binding
- TID 126:128 (= TID 141:143) binding in other chain
- D128 (= D143) active site, Proton acceptor; mutation to S: 18000-fold reduction of catalytic rate.
- R155 (≠ Q170) binding in other chain
- R163 (= R178) binding
- R172 (= R187) mutation to S: 3.4-fold reduction in turnover numbers.
- GCE 186:188 (≠ GAD 201:203) binding in other chain
- KKH 214:216 (≠ RNF 229:231) binding in other chain
- E223 (= E238) binding in other chain
- R244 (= R279) binding
- HIQR 250:253 (≠ HVQR 285:288) binding in other chain
5xz7A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
33% identity, 98% coverage: 5:357/361 of query aligns to 2:318/322 of 5xz7A
5xz6A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
33% identity, 98% coverage: 5:357/361 of query aligns to 2:318/318 of 5xz6A
- binding phosphoaminophosphonic acid-adenylate ester: S9 (= S12), G10 (= G13), G11 (= G14), Y41 (≠ T45), R72 (≠ P87), C73 (≠ M88), P74 (≠ A89), F76 (≠ G91), K77 (≠ T92), G101 (= G116), G102 (= G117), D103 (= D118), G104 (= G119), S105 (= S120), R107 (≠ A122), G108 (≠ I123), T127 (= T141), R173 (= R187)
5xz9A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
34% identity, 91% coverage: 5:334/361 of query aligns to 2:300/321 of 5xz9A
- binding adenosine-5'-triphosphate: S9 (= S12), G10 (= G13), Y41 (≠ T45), R72 (≠ P87), C73 (≠ M88), F76 (≠ G91), K77 (≠ T92), G102 (= G117), D103 (= D118), G104 (= G119), S105 (= S120), R107 (≠ A122), G108 (≠ I123), R173 (= R187)
5xzaA Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adp (see paper)
34% identity, 91% coverage: 5:334/361 of query aligns to 2:300/322 of 5xzaA