SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AZOBR_RS06995 FitnessBrowser__azobra:AZOBR_RS06995 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q58761 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
27% identity, 95% coverage: 12:293/297 of query aligns to 8:284/284 of Q58761

query
sites
Q58761

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DNE 34:36 (≠ DGE 38:40) binding
D212 (= D221) binding
STGGT 216:220 (≠ SSGET 225:229) binding

Q97Z86 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
29% identity, 89% coverage: 20:283/297 of query aligns to 16:278/291 of Q97Z86

query
sites
Q97Z86

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STGGT 220:224 (≠ SSGET 225:229) binding

1u9zA Crystal structure of phosphoribosyl diphosphate synthase complexed with amp and ribose 5-phosphate (see paper)
26% identity, 95% coverage: 12:293/297 of query aligns to 8:274/274 of 1u9zA

query
sites
1u9zA

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binding adenosine monophosphate: F32 (= F36), D34 (= D38), E36 (= E40), R92 (= R94), Q93 (= Q95), F97 (= F99), H125 (= H129)
binding ribose-5-phosphate: R92 (= R94), D163 (= D171), D202 (= D221), I204 (≠ V223), I205 (= I224), S206 (= S225), T207 (≠ S226), G208 (= G227), G209 (≠ E228), T210 (= T229)

4twbA Sulfolobus solfataricus ribose-phosphate pyrophosphokinase (see paper)
30% identity, 89% coverage: 20:283/297 of query aligns to 16:265/278 of 4twbA

query
sites
4twbA

L

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binding adenosine monophosphate: F32 (= F36), D34 (= D38), E36 (= E40), R93 (= R94), Q94 (= Q95), F98 (= F99), H127 (= H127)

3mbiA Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with adp-mg2+ and ribose 5- phosphate (see paper)
27% identity, 80% coverage: 17:254/297 of query aligns to 15:245/287 of 3mbiA

query
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3mbiA

A

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E

P

P

V

I

S

S

Q

S

A

Q

V

I

V

L

G

T

D

E

W

I

L

Y

G

S

R

S

L

Y

F

S

D

N

R

S

F

I

V

A

C

T

V

V

E

D

P

I

H
|
H

L

-

H

-

R

-

T

-

H

-

T

-

L

-

D

D

E

E

V

K

F

T

V

L

G

S

R

Y

P

S

S

K

V

V

C

K

L

F

S

S

G

D

A

L

G

H

A

A

I

N

A

D

E

A

R

I

L

V

R

R

R

Y

D

Y

G

K

T

N

A

V

P

D

D

V

A

D

V

Y

I

V

V

V

G

S

P

P

D

D

E

D

E

G

A

G

A

L

P

A

L

R

V

V

E

A

V

D

V

I

A

S

G

A

P

K

L

L

G

G

L

K

T

K

A

H

L

F

V

F

G

I

R

E

K
|
K

E

K

R
|
R

R

I

G

D

D

D

R

R

D

T

V

V

T

E

V

M

T

K

L

V

P

P

A

-

D

N

A

V

P

D

L

V

A

N

G

G

R

K

P

K

V

L

I

I

V

V

D

D

V

I

I
|
I

S
|
S

S
x
T

G
|
G

E
x
G

T
|
T

I

I

F

A

S

K

C

S

A

S

R

G

A

L

R

R

A

E

A

K

G

G

A

A

A

S

S

K

V

I

R

Y

V

V

F

S

G

A

V

V

H

H

A

G

L

L

F

F

binding adenosine-5'-diphosphate: F34 (= F36), D36 (= D38), E38 (= E40), R93 (= R94), Q94 (= Q95), Y98 (≠ F99), H126 (= H127), K186 (= K194), R188 (= R196)
binding magnesium ion: H95 (≠ D96), H126 (= H127)
binding phosphate ion: R93 (= R94), R188 (= R196), I215 (= I224), S216 (= S225), T217 (≠ S226), G218 (= G227), G219 (≠ E228), T220 (= T229)

3mbiD Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with adp-mg2+ and ribose 5- phosphate (see paper)
27% identity, 80% coverage: 17:254/297 of query aligns to 13:243/285 of 3mbiD

query
sites
3mbiD

A

A

R

A

R

R

L

I

A

A

E

E

A

E

L

L

N

K

V

T

P

E

C

P

H

V

I

M

A

P

E

D

L

E

H

R

R

R

F
|
F

P

P

D
|
D

G

G

E
|
E

S

L

L

Y

V

L

R

R

L

Y

P

D

E

E

A

D

V

L

E

T

R

G

A

H

V

N

V

I

Y

F

R

I

S

I

L

G

D

N

R

T

P

H

N

S

D

D

-

A

K

E

L

V

V

M

E

E

L

M

T

I

L

L

A

T

A

L

S

S

V

A

L

I

R

Q

R

D

H

Y

G

R

A

T

T

K

E

S

L

V

C

N

L

I

V

I

A

A

P

P

Y

Y

M

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D

H

A

Q

V

R

F
x
Y

R

K

P

N

G

G

E

E

P

P

V

I

S

S

Q

S

A

Q

V

I

V

L

G

T

D

E

W

I

L

Y

G

S

R

S

L

Y

F

S

D

N

R

S

F

I

V

A

C

T

V

V

E

D

P

I

H
|
H

L

-

H

-

R

-

T

-

H

-

T

-

L

-

D

D

E

E

V

K

F

T

V

L

G

S

R

Y

P

S

S

K

V

V

C

K

L

F

S

S

G

D

A

L

G

H

A

A

I

N

A

D

E

A

R

I

L

V

R

R

R

Y

D

Y

G

K

T

N

A

V

P

D

D

V

A

D

V

Y

I

V

V

V

G

S

P

P

D
|
D

E

D

E

G

A

G

A

L

P

A

L

R

V

V

E

A

V

D

V

I

A

S

G

A

P

K

L

L

G

G

L

K

T

K

A

H

L

F

V

F

G

I

R

E

K
|
K

E

K

R
|
R

R

I

G

D

D

D

R

R

D

T

V

V

T

E

V

M

T

K

L

V

P

P

A

-

D

N

A

V

P

D

L

V

A

N

G

G

R

K

P

K

V

L

I

I

V

V

D
|
D

V
x
I

I
|
I

S
|
S

S
x
T

G
|
G

E
x
G

T
|
T

I

I

F

A

S

K

C

S

A

S

R

G

A

L

R

R

A

E

A

K

G

G

A

A

A

S

S

K

V

I

R

Y

V

V

F

S

G

A

V

V

H

H

A

G

L

L

F

F

binding adenosine-5'-diphosphate: F32 (= F36), D34 (= D38), E36 (= E40), R91 (= R94), Q92 (= Q95), Y96 (≠ F99), H124 (= H127), K184 (= K194), R186 (= R196)
binding 5-O-phosphono-alpha-D-ribofuranose: R91 (= R94), H124 (= H127), D161 (= D171), K184 (= K194), R186 (= R196), D210 (= D221), D211 (= D222), I212 (≠ V223), I213 (= I224), S214 (= S225), T215 (≠ S226), G216 (= G227), G217 (≠ E228), T218 (= T229)

Q97CA5 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) (see paper)
27% identity, 80% coverage: 17:254/297 of query aligns to 13:243/286 of Q97CA5

query
sites
Q97CA5

A

A

R

A

R

R

L

I

A

A

E

E

A

E

L

L

N

K

V

T

P

E

C

P

H

V

I

M

A

P

E

D

L

E

H

R

R

R

F

F

P

P

D
|
D

G
|
G

E
|
E

S

L

L

Y

V

L

R

R

L

Y

P

D

E

E

A

D

V

L

E

T

R

G

A

H

V

N

V

I

Y

F

R

I

S

I

L

G

D

N

R

T

P

H

N

S

D

D

-

A

K

E

L

V

V

M

E

E

L

M

T

I

L

L

A

T

A

L

S

S

V

A

L

I

R

Q

R

D

H

Y

G

R

A

T

T

K

E

S

L

V

C

N

L

I

V

I

A

A

P

P

Y

Y

M

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D
x
H

A

Q

V

R

F

Y

R

K

P

N

G

G

E

E

P

P

V

I

S

S

Q

S

A

Q

V

I

V

L

G

T

D

E

W

I

L

Y

G

S

R

S

L

Y

F

S

D

N

R

S

F

I

V

A

C

T

V

V

E

D

P

I

H

H

L

-

H

-

R

-

T

-

H

-

T

-

L

-

D

D

E

E

V

K

F

T

V

L

G

S

R

Y

P

S

S

K

V

V

C

K

L

F

S

S

G

D

A

L

G

H

A

A

I

N

A

D

E

A

R

I

L

V

R

R

R

Y

D

Y

G

K

T

N

A

V

P

D

D

V

A

D

V

Y

I

V

V

V

G

S

P

P

D

D

E

D

E

G

A

G

A

L

P

A

L

R

V

V

E

A

V

D

V

I

A

S

G

A

P

K

L

L

G

G

L

K

T

K

A

H

L

F

V

F

G

I

R

E

K

K

E

K

R
|
R

R

I

G

D

D

D

R

R

D

T

V

V

T

E

V

M

T

K

L

V

P

P

A

-

D

N

A

V

P

D

L

V

A

N

G

G

R

K

P

K

V

L

I

I

V

V

D
|
D

D

D

V

I

I

I

S
|
S

S
x
T

G
|
G

E
x
G

T
|
T

I

I

F

A

S

K

C

S

A

S

R

G

A

L

R

R

A

E

A

K

G

G

A

A

A

S

S

K

V

I

R

Y

V

V

F

S

G

A

V

V

H

H

A

G

L

L

F

F

DGE 34:36 (= DGE 38:40) binding
RQH 91:93 (≠ RQD 94:96) binding
R186 (= R196) binding
D210 (= D221) binding
STGGT 214:218 (≠ SSGET 225:229) binding

3lpnA Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with an atp analog (ampcpp). (see paper)
27% identity, 80% coverage: 17:254/297 of query aligns to 13:243/284 of 3lpnA

query
sites
3lpnA

A

A

R

A

R
|
R

L

I

A

A

E

E

A

E

L

L

N

K

V

T

P

E

C

P

H

V

I

M

A

P

E

D

L

E

H

R

R

R

F
|
F

P

P

D
|
D

G

G

E
|
E

S

L

L

Y

V

L

R

R

L

Y

P

D

E

E

A

D

V

L

E

T

R

G

A

H

V

N

V

I

Y

F

R

I

S

I

L

G

D

N

R

T

P

H

N
x
S

D

D

-
x
A

K
x
E

L

V

V

M

E

E

L

M

T

I

L

L

A

T

A

L

S

S

V

A

L

I

R

Q

R

D

H

Y

G

R

A

T

T

K

E

S

L

V

C

N

L

I

V

I

A

A

P

P

Y

Y

M

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D
x
H

A

Q

V

R

F
x
Y

R

K

P

N

G

G

E

E

P

P

V

I

S

S

Q

S

A

Q

V

I

V

L

G

T

D

E

W

I

L

Y

G

S

R

S

L

Y

F

S

D

N

R

S

F

I

V

A

C

T

V

V

E

D

P

I

H
|
H

L

-

H

-

R

-

T

-

H

-

T

-

L

-

D
|
D

E

E

V

K

F

T

V

L

G

S

R

Y

P

S

S

K

V

V

C

K

L

F

S

S

G

D

A

L

G

H

A

A

I

N

A

D

E

A

R

I

L

V

R

R

R

Y

D

Y

G

K

T

N

A

V

P

D

D

V

A

D

V

Y

I

V

V

V

G

S

P

P

D

D

E

D

E

G

A

G

A

L

P

A

L

R

V

V

E

A

V

D

V

I

A

S

G

A

P

K

L

L

G

G

L

K

T

K

A

H

L

F

V

F

G

I

R

E

K

K

E

K

R

R

R

I

G

D

D

D

R

R

D

T

V

V

T

E

V

M

T

K

L

V

P

P

A

-

D

N

A

V

P

D

L

V

A

N

G

G

R

K

P

K

V

L

I

I

V

V

D

D

V

I

I
|
I

S
|
S

S
x
T

G
|
G

E
x
G

T
|
T

I

I

F

A

S

K

C

S

A

S

R

G

A

L

R

R

A

E

A

K

G

G

A

A

A

S

S

K

V

I

R

Y

V

V

F

S

G

A

V

V

H

H

A

G

L

L

F

F

binding diphosphomethylphosphonic acid adenosyl ester: F32 (= F36), D34 (= D38), E36 (= E40), R91 (= R94), Q92 (= Q95), H93 (≠ D96), Y96 (≠ F99), H124 (= H127), D125 (= D135)
binding sulfate ion: R15 (= R19), S58 (≠ N62), A60 (vs. gap), E61 (≠ K64), R91 (= R94), I213 (= I224), S214 (= S225), T215 (≠ S226), G216 (= G227), G217 (≠ E228), T218 (= T229)

Sites not aligning to the query:

binding sulfate ion: 7, 11

P0A717 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Escherichia coli (strain K12) (see 4 papers)
28% identity, 88% coverage: 17:277/297 of query aligns to 16:276/315 of P0A717

query
sites
P0A717

A

A

R

Q

R

R

L

I

A

A

E

N

A

R

L

L

N

Y

V

T

P

S

C

L

H

G

I

D

A

A

E

A

L

V

H

G

R

R

F

F

P

S

D

D

G

G

E

E

S

V

L

S

V

V

R

Q

L

I

P

N

E

E

A

N

V

V

-

R

-

G

E

D

R

I

A

F

V

I

Y

Q

R

S

S

T

L

C

D

A

R

P

P

T

N

N

D

D

K

N

L

L

V

M

E

E

L

L

T

V

L

V

A

M

A

V

S

D

V

A

L

L

R

R

H

A

G

S

A

A

T

G

E

R

L

I

C

T

L

A

V

V

A

I

P

P

Y

Y

M

F

A

G

Y

Y

M

A

R

R

Q

Q

D

D

A

R

V

R

F

V

R

R

P

S

G

A

E

R

-

V

P

P

V

I

S

T

Q

A

A

K

V

V

G

A

D

D

W

F

L

L

G

S

R

S

L

V

-

G

F

V

D

D

R

R

F

V

V

L

C

T

V

V

E
x
D

P

L

H

H

L

A

H

E

R

Q

T

I

H

Q

T

G

L

F

-

F

-

D

-

V

-

P

-

V

D

D

E

N

V

V

F

F

V

-

G

G

R

S

P

P

S

I

V

L

C

-

L

-

S

-

G

-

A

-

G

-

A

-

I

L

A

E

E

D

R

M

L

L

R

Q

R

L

D

N

G

L

T

D

A

N

P

P

D

-

A

-

V

I

I

V

V

V

G

S

P

P

D

D

E

I

E

G

A

G

A

V

P

V

L

R

V

A

E

R

V

A

V

I

A

A

G

K

P

L

L

L

G

N

L

D

T

T

A

D

L

M

V

A

G

I

R

I

K

D

E

K

R

R

G

P

D

R

R

A

D

N

V

V

T

S

V

Q

T

V

L

M

P

H

A

I

D

I

A

G

P

D

L

V

A

A

G

G

R

R

P

D

V

C

V

V

I

L

V

V

D
|
D

V

M

I

I

S
x
D

S

T

G

G

E

G

T

T

I

L

F

C

S

K

C

A

A

A

R

E

A

A

A

L

R

K

A

E

A

R

G

G

A

A

A

K

S

R

V

V

R

F

V

A

F

Y

G

A

V

T

H

H

A

P

L

I

F

F

D

S

A

G

A

N

V

A

A

A

A

N

R

N

F

L

A

R

A

N

E

S

G

V

L

I

G

D

T

E

P

V

L

V

S

V

C

C

D

D

G

T

V

I

P

P

D129 (≠ E125) to A: in mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP
D220 (= D221) mutation to E: 4-fold decrease in the affinity binding for Rib-5-P in the presence of magnesium ions. In the presence of cobalt ions, it shows a 15-fold decrease in the affinity binding for Rib-5-P.; mutation to F: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.
D221 (= D222) mutation to A: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions.
D224 (≠ S225) mutation to A: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.; mutation to S: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6asvC E. Coli prpp synthetase (see paper)
28% identity, 88% coverage: 17:277/297 of query aligns to 14:274/311 of 6asvC

query
sites
6asvC

A

A

R

Q

R

R

L

I

A

A

E

N

A

R

L

L

N

Y

V

T

P

S

C

L

H

G

I

D

A

A

E

A

L

V

H

G

R

R

F

F

P

S

D

D

G

G

E

E

S

V

L

S

V

V

R

Q

L

I

P

N

E

E

A

N

V

V

-

R

-

G

E

D

R

I

A

F

V

I

Y

Q

R

S

S

T

L

C

D

A

R

P

P

T

N

N

D

D

K

N

L

L

V

M

E

E

L

L

T

V

L

V

A

M

A

V

S

D

V

A

L

L

R

R

H

A

G

S

A

A

T

G

E

R

L

I

C

T

L

A

V

V

A

I

P

P

Y

Y

M

F

A

G

Y

Y

M

A

R

R

Q

Q

D

D

A

R

V

R

F

V

R

R

P

S

G

A

E

R

-

V

P

P

V

I

S

T

Q

A

A

K

V

V

G

A

D

D

W

F

L

L

G

S

R

S

L

V

-

G

F

V

D

D

R

R

F

V

V

L

C

T

V

V

E

D

P

L

H
|
H

L

A

H

E

R

Q

T

I

H

Q

T

G

L

F

-

F

-

D

-

V

-

P

-

V

D

D

E

N

V

V

F

F

V

-

G

G

R

S

P

P

S

I

V

L

C

-

L

-

S

-

G

-

A

-

G

-

A

-

I

L

A

E

E

D

R

M

L

L

R

Q

R

L

D

N

G

L

T

D

A

N

P

P

D

-

A

-

V

I

I

V

V

V

G

S

P

P

D
|
D

E

I

E
x
G

A
x
G

A

V

P

V

L

R

V

A

E

R

V

A

V

I

A

A

G

K

P

L

L

L

G

N

L

D

T

T

A

D

L

M

V

A

G

I

R

I

K

D

E

K

R

R

G

P

D

R

R

A

D

N

V

V

T

S

V

Q

T

V

L

M

P

H

A

I

D

I

A

G

P

D

L

V

A

A

G

G

R

R

P

D

V

C

V

V

I

L

V

V

D
|
D

V

M

I

I

S

D

S

T

G

G

E

G

T

T

I

L

F

C

S

K

C

A

A

A

R

E

A

A

A

L

R

K

A

E

A

R

G

G

A

A

A

K

S

R

V

V

R

F

V

A

F

Y

G

A

V

T

H

H

A

P

L

I

F

F

D

S

A

G

A

N

V

A

A

A

A

N

R

N

F

L

A

R

A

N

E

S

G

V

L

I

G

D

T

E

P

V

L

V

S

V

C

C

D

D

G

T

V

I

P

P

binding phosphate ion: H129 (= H127), D168 (= D171), G170 (≠ E173), G171 (≠ A174), D218 (= D221), D219 (= D222)

4s2uA Crystal structure of the phosphorybosylpyrophosphate synthetase from e. Coli
29% identity, 88% coverage: 17:277/297 of query aligns to 15:275/308 of 4s2uA

query
sites
4s2uA

A

A

R

Q

R

R

L

I

A

A

E

N

A

R

L

L

N

Y

V

T

P

S

C

L

H

G

I

D

A

A

E

A

L

V

H

G

R

R

F

F

P

S

D

D

G

G

E

E

S

V

L

S

V

V

R

Q

L

I

P

N

E

E

A

N

V

V

E

R

R

G

A

G

V

D

V

I

Y

F

-

I

R

Q

S

S

L

T

D

C

R

A

P

P

-

T

N

N

D

D

K

N

L

L

V

M

E

E

L

L

T

V

L

V

A

M

A

V

S

D

V

A

L

L

R

R

H

A

G

S

A

A

T

G

E

R

L

I

C

T

L

A

V

V

A

I

P

P

Y

Y

M

F

A

G

Y

Y

M

A

R

R

Q

Q

D

D

A

R

V

R

F

V

R

R

P

S

G

A

E

R

-

V

P

P

V

I

S

T

Q

A

A

K

V

V

G

A

D

D

W

F

L

L

G

S

R

S

L

V

-

G

F

V

D

D

R

R

F

V

V

L

C

T

V

V

E

D

P

L

H

H

L

A

H

E

R

Q

T

I

H

Q

T

G

L

F

-

F

-

D

-

V

-

P

-

V

D

D

E

N

V

V

F

F

V

-

G

G

R

S

P

P

S

I

V

L

C

-

L

-

S

-

G

-

A

-

G

-

A

-

I

L

A

E

E

D

R

M

L

L

R

Q

R

L

D

N

G

L

T

D

A

N

P

P

D

-

A

-

V

I

I

V

V

V

G

S

P

P

D

D

E

I

E

G

A

G

A

V

P

V

L

R

V

A

E

R

V

A

V

I

A

A

G

K

P

L

L

L

G

N

L

D

T

T

A

D

L

M

V

A

G

I

R

I

K

D

E

K

R

R

G

P

D

R

R

A

D

N

V

V

T

S

V

Q

T

V

L

M

P

H

A

I

D

I

A

G

P

D

L

V

A

A

G

G

R

R

P

D

V

C

V

V

I

L

V

V

D

D

V

M

I

I

S
x
D

S

T

G

G

E

G

T
|
T

I

L

F

C

S

K

C

A

A

A

R

E

A

A

A

L

R

K

A

E

A

R

G

G

A

A

A

K

S

R

V

V

R

F

V

A

F

Y

G

A

V

T

H

H

A

P

L

I

F

F

D

S

A

G

A

N

V

A

A

A

A

N

R

N

F

L

A

R

A

N

E

S

G

V

L

I

G

D

T

E

P

V

L

V

S

V

C

C

D

D

G

T

V

I

P

P

binding magnesium ion: D223 (≠ S225), T227 (= T229)

7xmvA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a(amp/adp) filament bound with adp, amp and r5p (see paper)
28% identity, 88% coverage: 17:277/297 of query aligns to 14:268/307 of 7xmvA

query
sites
7xmvA

A

A

R

Q

R

R

L

I

A

A

E

N

A

R

L

L

N

Y

V

T

P

S

C

L

H

G

I

D

A

A

E

A

L

V

H

G

R

R

F
|
F

P

S

D
|
D

G

G

E
|
E

S

V

L

S

V

V

R

Q

L

I

P

N

E

E

A

N

V

V

E

R

R

G

A

G

V

D

V

I

Y

F

-

I

R

Q

S

S

L

T

D

C

R

A

P

P

-

T

N

N

D

D

K

N

L

L

V

M

E

E

L

L

T

V

L

V

A

M

A

V

S

D

V

A

L

L

R

R

H

A

G

S

A

A

T

G

E

R

L

I

C

T

L

A

V

V

A

I

P

P

Y

Y

M

F

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D

D

A
x
R

V

R

F
x
V

R
|
R

P

S

G

A

E

R

-

V

P

P

V

I

S

T

Q

A

A

K

V

V

G

A

D

D

W

F

L

L

G

S

R

S

L

V

-

G

F

V

D

D

R

R

F

V

V

L

C

T

V

V

E

D

P

L

H
|
H

L

A

H
x
E

R

Q

T

I

H

Q

T

G

L

F

-

F

-

D

-

V

-

P

-

V

D

D

E

N

V

V

F
|
F

V

-

G

G

R
x
S

P

P

S

I

V

L

C

-

L

-

S

-

G

-

A

-

G

-

A

-

I

L

A

E

E

D

R

M

L

L

R

Q

R

L

D

N

G

L

T

D

A

N

P

P

D

-

A

-

V

I

I

V

V

V

G

S

P

P

D
|
D

E

I

E

G

A

G

A

V

P
x
V

L

R

V

A

E

R

V
x
A

V

I

A

A

G

K

P

L

L

L

G

N

L

D

T

T

A

D

L

M

V

A

G

I

R

I

K

D

E

K

R

R

G

Q

D

-

R

-

D

-

V

-

T

-

V

-

T

V

L

M

P

H

A

I

D

I

A

G

P

D

L

V

A

A

G

G

R

R

P

D

V

C

V

V

I

L

V

V

D
|
D

V
x
M

I

I

S
x
D

S
x
T

G
|
G

E
x
G

T
|
T

I

L

F

C

S

K

C

A

A

A

R

E

A

A

A

L

R

K

A

E

A

R

G

G

A

A

A

K

S

R

V

V

R

F

V

A

F

Y

G

A

V

T

H

H

A

P

L

I

F

F

D

S

A

G

A

N

V

A

A

A

A

N

R

N

F

L

A

R

A

N

E

S

G

V

L

I

G

D

T

E

P

V

L

V

S

V

C

C

D

D

G

T

V

I

P

P

binding adenosine-5'-diphosphate: F33 (= F36), D35 (= D38), E37 (= E40), R94 (= R94), Q95 (= Q95), R97 (≠ A97), H129 (= H127)
binding adenosine monophosphate: R97 (≠ A97), V99 (≠ F99), R100 (= R100), E131 (≠ H129), F145 (= F138), S147 (≠ R141), V173 (≠ P176), A177 (≠ V180)
binding 5-O-phosphono-alpha-D-ribofuranose: R94 (= R94), H129 (= H127), D168 (= D171), D212 (= D221), D213 (= D222), M214 (≠ V223), D216 (≠ S225), T217 (≠ S226), G218 (= G227), G219 (≠ E228), T220 (= T229)
binding magnesium ion: D168 (= D171), D212 (= D221)

7xmuA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a filament bound with adp, pi and r5p (see paper)
28% identity, 88% coverage: 17:277/297 of query aligns to 14:268/307 of 7xmuA

query
sites
7xmuA

A

A

R

Q

R

R

L

I

A

A

E

N

A

R

L

L

N

Y

V

T

P

S

C

L

H

G

I

D

A

A

E

A

L

V

H

G

R

R

F
|
F

P

S

D
|
D

G

G

E
|
E

S

V

L

S

V

V

R

Q

L

I

P

N

E

E

A
x
N

V

V

E
x
R

R

G

A

G

V

D

V

I

Y

F

-

I

R

Q

S

S

L

T

D

C

R

A

P

P

-

T

N

N

D

D

K

N

L

L

V

M

E

E

L

L

T

V

L

V

A

M

A

V

S

D

V

A

L

L

R

R

H
x
A

G

S

A

A

T

G

E

R

L

I

C

T

L

A

V

V

A

I

P

P

Y

Y

M

F

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D

D

A
x
R

V

R

F
x
V

R
|
R

P

S

G

A

E

R

-

V

P

P

V

I

S

T

Q

A

A

K

V

V

G

A

D

D

W

F

L

L

G

S

R

S

L

V

-

G

F

V

D

D

R

R

F

V

V

L

C

T

V

V

E

D

P

L

H
|
H

L

A

H
x
E

R

Q

T

I

H

Q

T

G

L

F

-

F

-

D

-

V

-

P

-

V

D

D

E

N

V

V

F
|
F

V

-

G

G

R
x
S

P

P

S

I

V

L

C

-

L

-

S

-

G

-

A

-

G

-

A

-

I

L

A

E

E

D

R

M

L

L

R

Q

R

L

D

N

G

L

T

D

A

N

P

P

D

-

A

-

V

I

I

V

V

V

G

S

P

P

D
|
D

E

I

E

G

A

G

A

V

P
x
V

L

R

V

A

E
x
R

V
x
A

V

I

A

A

G
x
K

P

L

L

L

G

N

L

D

T

T

A

D

L

M

V

A

G

I

R

I

K

D

E

K

R

R

G

Q

D

-

R

-

D

-

V

-

T

-

V

-

T

V

L

M

P

H

A

I

D

I

A

G

P

D

L

V

A

A

G

G

R

R

P

D

V

C

V

V

I

L

V

V

D
|
D

V
x
M

I

I

S
x
D

S
x
T

G
|
G

E

G

T
|
T

I

L

F

C

S

K

C

A

A

A

R

E

A

A

A

L

R

K

A

E

A

R

G

G

A

A

A

K

S

R

V

V

R

F

V

A

F

Y

G

A

V

T

H

H

A

P

L

I

F

F

D

S

A

G

A

N

V

A

A

A

A

N

R

N

F

L

A

R

A

N

E

S

G

V

L

I

G

D

T

E

P

V

L

V

S

V

C

C

D

D

G

T

V

I

P

P

binding adenosine-5'-diphosphate: F33 (= F36), D35 (= D38), E37 (= E40), R94 (= R94), Q95 (= Q95), R97 (≠ A97), R97 (≠ A97), V99 (≠ F99), R100 (= R100), H129 (= H127), E131 (≠ H129), F145 (= F138), S147 (≠ R141), V173 (≠ P176), R176 (≠ E179), A177 (≠ V180), K180 (≠ G183)
binding 5-O-phosphono-alpha-D-ribofuranose: H129 (= H127), D168 (= D171), D212 (= D221), D213 (= D222), M214 (≠ V223), D216 (≠ S225), T217 (≠ S226), G218 (= G227), T220 (= T229)
binding phosphate ion: N45 (≠ A48), R47 (≠ E50), A78 (≠ H78)

P9WKE3 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
29% identity, 84% coverage: 17:266/297 of query aligns to 24:275/326 of P9WKE3

query
sites
P9WKE3

A

A

R

E

R

Q

L

V

A

A

E
x
K

A

E

L

L

N

D

V

V

P

H

C

V

H

T

I

S

A

Q

E

D

L

A

H

R

R

E

F

F

P

A

D

N

G

G

E

E

S

I

L

F

V

V

R

R

L

F

P

H

E

E

A

S

V

V

E

R

-

G

-

C

R

D

A

A

V

F

V

V

Y

L

R

Q

S

S

L

C

D

P

R

A

P

P

-

V

N

N

D

R

K

W

L

L

V

M

E

E

L

Q

T

L

L

I

A

M

A

I

S

D

V

A

L

L

R

K

R

R

H

G

G

S

A

A

T

K

E

R

L

I

C

T

L

A

V

V

A

M

P

P

Y

F

M

Y

A

P

Y

Y

M

A

R

R

Q

Q

D

D

A

K

V

K

F

H

R

R

P

G

G

R

E

E

P

P

V

I

S

S

Q

A

A

R

V

L

V

I

G

A

D

D

W

L

L

L

G

K

R

T

L

A

-

G

F

A

D

D

R

R

F

I

V

V

C

T

V

V

E

D

P

L

H

H

L

-

H

-

R

-

T

T

H

D

T

Q

L

I

D

Q

E

G

V

F

F

F

V

D

G

G

R

-

P

P

S

V

V

D

C

H

L

M

S

R

G

G

A

Q

G

N

A

L

I

L

A

T

E

G

R

Y

L

I

R

R

R

D

D

N

G

Y

T

P

A

D

P

G

D

N

A

M

V

V

I

V

V

V

G

S

P

P

D

D

E

S

E

G

A

R

A

V

P

R

L

I

V

A

E

E

V

K

V

W

A

A

G

D

P

A

L

L

G

G

L

G

T

V

A

P

L

L

V

A

G

F

R

I

K

H

E

K

R

T

R

R

G

D

D

P

R

R

-

V

-

P

D

N

V

Q

T

V

V

V

T

S

L

N

P

R

A

V

D

V

A

G

P

D

L

V

A

A

G

G

R

R

P

T

V

C

V

V

I

L

V

I

D

D

V

M

I

I

S

D

S

T

G

G

E

G

T

T

I

I

F

A

S

G

C

A

A

V

R

A

A

L

R

H

A

N

A

D

G

G

A

A

A

G

S

D

V

V

R

I

V

I

F

A

G

A

V

T

H

H

A

G

L

V

F

L

D

S

A

D

A

P

V

A

A

A

A

Q

R

R

F

L

A

A

A

S

E

C

G

G

K29 (≠ E22) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Q63XL8 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Burkholderia pseudomallei (strain K96243) (see paper)
26% identity, 95% coverage: 1:283/297 of query aligns to 1:284/318 of Q63XL8

query
sites
Q63XL8

M

M

A

S

R

S

Y

H

T

D

G

G

V

L

Y

M

G

V

F

F

P

T

E

G

S

N

A

A

D

N

G

P

A

A

-

L

-

A

R

Q

R

E

L

V

A

V

E

K

A

I

L

L

N

G

V

I

P

P

C

L

H

G

I

K

A

A

E

M

L

V

H

S

R

R

F

F

P

S

D

D

G

G

E

E

S

I

L

Q

V

V

R

E

L

I

P

Q

E

E

A

N

V

V

-

R

-

G

E

K

R

D

A

V

V

F

V

V

Y

L

R

Q

S

S

L

T

D

C

R

A

P

P

-

T

N

N

D

D

K

N

L

L

V

M

E

E

L

L

T

M

L

I

A

M

A

V

S

D

V

A

L

L

R

K

R

R

H

A

G

S

A

A

T

G

E

R

L

I

C

T

L

A

V

A

A

I

P

P

Y

Y

M

F

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D

D

A

R

V

R

F

P

R

R

P

S

G

A

E

R

-

V

P

A

V

I

S

S

Q

A

A

K

V

V

G

A

D

N

W

M

L

L

G

E

R

I

L

A

-

G

F

V

D

E

R

R

F

I

V

I

C

T

V

M

E

D

P

L

H

H

L

A

H

D

R

Q

T

I

H

Q

T

G

L

-

D

-

E

-

V

-

F

F

V

F

G

D

R

I

P

P

S

V

V

D

C

N

L

I

S

Y

G

A

A

T

G

P

A

I

I

L

A

L

E

G

R

D

L

L

R

R

R

K

D

Q

G

N

T

Y

A

-

P

P

D

D

A

L

V

L

I

V

V

V

G

S

P

P

D

D

E

V

E

G

A

G

A

V

P

V

L

R

V

A

E

R

V

A

V

L

A

A

G

K

P

Q

L

L

G

N

L

C

T

D

A

L

L

A

V

I

G

I

R

D

K

K

E

-

R

R

G

P

D

K

R

A

D

N

V

V

T

A

V

E

T

V

L

M

P

N

A

I

D

I

A

G

P

E

L

V

A

E

G

G

R

R

P

T

V

C

V

V

I

I

V

M

D

D

V

M

I

V

S

D

S

T

G

A

E

G

T

T

I

L

F

C

S

K

C

A

A

A

R

Q

A

V

A

L

R

K

A

E

A

R

G

G

A

A

A

K

S

Q

V

V

R

F

V

A

F

Y

G

A

V

T

H

H

A

P

L

V

F

L

D

S

A

G

V

A

A

A

A

D

R

R

F

I

A

A

E

S

G

A

L

L

G

D

T

E

P

L

L

V

S

V

C

T

D

D

G

T

V

I

P

P

H

L

P

S

S

A

N

E

D

S

L

L

RQ 99:100 (= RQ 94:95) binding

6nfeB Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
26% identity, 88% coverage: 17:277/297 of query aligns to 15:270/299 of 6nfeB

query
sites
6nfeB

A

A

R

L

R

K

L

I

A

S

E

S

A

H

L

L

N

Q

V

I

P

P

C

I

H

G

I

K

A

A

E

T

L

V

H

G

R

T

F
|
F

P

S

D
|
D

G

G

E
|
E

S

T

L

M

V

V

R

E

L

I

P

L

E

E

A

N

V

V

-

R

-

G

E

K

R

D

A

V

V

F

V

V

Y

L

R

Q

S

S

L

T

D

C

R

A

P

P

-

A

N

N

D

N

K

N

L

L

V

M

E

E

L

L

T

L

L

I

A

M

A

A

S

D

V

A

L

L

R

R

H

S

G

S

A

A

T

G

E

R

L

I

C

T

L

A

V

V

A

V

P

P

Y

Y

M

F

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D

D

A
x
R

V

R

F

V

R

R

P

S

G

A

E

R

-

V

P

P

V

I

S

T

Q

A

A

K

V

V

G

A

D

D

W

M

L

M

G

A

R

S

L

V

-

G

F

I

D

C

R

R

F

V

V

L

C

T

V

V

E

D

P

L

H
|
H

L

A

H

D

R

Q

T

I

H

Q

T

G

L

-

D

-

E

-

V

-

F

F

V

F

G

Y

R

M

P

P

S

V

V

D

C

N

L

V

S

Y

G

S

A

T

G

P

A

V

I

L

A

L

E

E

R

D

L

I

R

T

R

K

D

Q

G

K

T

L

A

-

P

N

D

N

A

I

V

M

I

I

V

V

G

S

P

P

D
|
D

E

V

E

G

A

G

A

V

P

V

L

R

V

A

E

R

V

A

V

V

A

A

G

K

P

R

L

L

G

N

L

D

T

A

A

E

L

L

V

S

G

I

R

I

K

D

E

K

R

R

G

S

D

E

R

V

D

M

V

H

T

I

V

I

T

G

L

E

P

P

A

A

D

N

A

-

P

-

L

-

A

-

G

-

R

K

P

N

V

C

V

I

I

I

V

V

D
|
D

V
x
I

I
x
V

S
x
D

S
x
T

G
x
A

E
x
G

T
|
T

I

L

F

C

S

T

C

A

A

A

R

H

A

E

A

L

R

K

A

K

A

N

G

G

A

A

A

K

S

S

V

V

R

R

V

A

F

Y

G

I

V

T

H

H

A

P

L

V

F

L

D

S

A

G

A

P

V

A

A

V

A

N

R

N

F

I

A

K

A

H

E

S

G

G

L

L

G

D

T

E

P

V

L

V

S

V

C

T

D

D

G

T

V

I

P

P

binding adenosine-5'-diphosphate: F34 (= F36), D36 (= D38), E38 (= E40), R95 (= R94), Q96 (= Q95), R98 (≠ A97), H130 (= H127)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H127), D169 (= D171), D214 (= D221), D215 (= D222), I216 (≠ V223), V217 (≠ I224), D218 (≠ S225), T219 (≠ S226), A220 (≠ G227), G221 (≠ E228), T222 (= T229)

6nfeA Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
26% identity, 88% coverage: 17:277/297 of query aligns to 15:269/298 of 6nfeA

query
sites
6nfeA

A

A

R

L

R

K

L

I

A

S

E

S

A

H

L

L

N

Q

V

I

P

P

C

I

H

G

I

K

A

A

E

T

L

V

H

G

R

T

F
|
F

P

S

D
|
D

G

G

E
|
E

S

T

L

M

V

V

R

E

L

I

P

L

E

E

A

N

V

V

-

R

-

G

E

K

R

D

A

V

V

F

V

V

Y

L

R

Q

S

S

L

T

D

C

R

A

P

P

-

A

N

N

D

N

K

N

L

L

V

M

E

E

L

L

T

L

L

I

A

M

A

A

S

D

V

A

L

L

R

R

H

S

G

S

A

A

T

G

E

R

L

I

C

T

L

A

V

V

A

V

P

P

Y

Y

M

F

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D

D

A
x
R

V

R

F
x
V

R
|
R

P

S

G

A

E

R

-

V

P

P

V

I

S

T

Q

A

A

K

V

V

G

A

D

D

W

M

L

M

G

A

R

S

L

V

-

G

F

I

D

C

R

R

F

V

V

L

C

T

V

V

E

D

P

L

H
|
H

L

A

H

D

R

Q

T

I

H

Q

T

G

L

-

D

-

E

-

V

-

F

F

V

F

G

Y

R

M

P

P

S

V

V

D

C

N

L

V

S
x
Y

G

S

A
x
T

G

P

A

V

I

L

A

L

E

E

R

D

L

I

R

T

R

K

D

Q

G

-