SitesBLAST
Comparing AZOBR_RS08460 FitnessBrowser__azobra:AZOBR_RS08460 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3bptA Crystal structure of human beta-hydroxyisobutyryl-coa hydrolase in complex with quercetin
47% identity, 100% coverage: 2:358/358 of query aligns to 1:357/362 of 3bptA
- active site: G67 (= G72), P84 (≠ A92), R88 (≠ S99), G115 (= G126), G118 (= G129), E138 (= E149), D146 (= D157)
- binding (2r)-3-hydroxy-2-methylpropanoic acid: G66 (= G71), G67 (= G72), I69 (≠ V74), E90 (= E101), G114 (= G125), G115 (= G126), E138 (= E149), D146 (= D157), V147 (= V158)
- binding 3,5,7,3',4'-pentahydroxyflavone: F25 (≠ A30), L26 (= L31), A28 (= A33), G66 (= G71), G67 (= G72), I69 (≠ V74), P137 (= P148), I141 (= I152), L319 (= L320)
4hdtA Crystal structure of a carnitinyl-coa dehydratase from mycobacterium thermoresistibile (see paper)
48% identity, 97% coverage: 11:356/358 of query aligns to 3:337/340 of 4hdtA
- active site: G64 (= G72), I69 (≠ L77), W84 (≠ F98), Y88 (= Y102), G112 (= G126), G115 (= G129), E135 (= E149), P142 (= P156), D143 (= D157), R283 (≠ I297)
- binding zinc ion: H28 (≠ L36), E42 (≠ I50), E57 (= E65), E79 (≠ S93), H93 (≠ L107), H185 (≠ S199)
Sites not aligning to the query:
Q9LKJ1 3-hydroxyisobutyryl-CoA hydrolase 1; CoA-thioester hydrolase CHY1; EC 3.1.2.-; EC 3.1.2.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
42% identity, 94% coverage: 10:346/358 of query aligns to 9:353/378 of Q9LKJ1
- G70 (= G72) mutation to S: Loss of activity.
- E142 (= E149) mutation to A: Loss of activity.
- D150 (= D157) mutation to G: Reduced activity.
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
30% identity, 60% coverage: 7:220/358 of query aligns to 1:205/259 of 5zaiC
- active site: A65 (≠ G72), F70 (≠ L77), S82 (≠ A84), R86 (≠ Q88), G110 (= G126), E113 (≠ G129), P132 (= P148), E133 (= E149), I138 (≠ L154), P140 (= P156), G141 (≠ D157)
- binding coenzyme a: K24 (≠ A30), L25 (= L31), A63 (= A70), G64 (= G71), A65 (≠ G72), D66 (= D73), I67 (≠ V74), P132 (= P148), R166 (= R181)
Sites not aligning to the query:
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
34% identity, 56% coverage: 8:208/358 of query aligns to 2:191/260 of 2hw5C
- active site: A68 (≠ G72), M73 (≠ L77), S83 (= S99), L87 (≠ V103), G111 (= G126), E114 (≠ G129), P133 (= P148), E134 (= E149), T139 (≠ L154), P141 (= P156), G142 (≠ D157)
- binding crotonyl coenzyme a: K26 (= K29), A27 (= A30), L28 (= L31), A30 (= A33), K62 (= K66), I70 (≠ V74), F109 (≠ M124)
Sites not aligning to the query:
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
35% identity, 56% coverage: 8:208/358 of query aligns to 2:191/260 of 1dubA
- active site: A68 (≠ G72), M73 (≠ L77), S83 (≠ A86), L87 (≠ F98), G111 (= G126), E114 (≠ G129), P133 (= P148), E134 (= E149), T139 (≠ L154), P141 (= P156), G142 (≠ D157)
- binding acetoacetyl-coenzyme a: K26 (= K29), A27 (= A30), L28 (= L31), A30 (= A33), A66 (= A70), A68 (≠ G72), D69 (= D73), I70 (≠ V74), Y107 (≠ I122), G110 (= G125), G111 (= G126), E114 (≠ G129), P133 (= P148), E134 (= E149), L137 (≠ I152), G142 (≠ D157)
Sites not aligning to the query:
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
35% identity, 56% coverage: 8:208/358 of query aligns to 32:221/290 of P14604
- E144 (≠ G129) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E149) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
35% identity, 56% coverage: 9:208/358 of query aligns to 1:189/258 of 1ey3A
- active site: A66 (≠ G72), M71 (≠ L77), S81 (≠ A86), L85 (≠ F98), G109 (= G126), E112 (≠ G129), P131 (= P148), E132 (= E149), T137 (≠ L154), P139 (= P156), G140 (≠ D157)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (= K29), L26 (= L31), A28 (= A33), A64 (= A70), G65 (= G71), A66 (≠ G72), D67 (= D73), I68 (≠ V74), L85 (≠ F98), W88 (≠ E101), G109 (= G126), P131 (= P148), L135 (≠ I152), G140 (≠ D157)
Sites not aligning to the query:
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
34% identity, 56% coverage: 8:208/358 of query aligns to 1:185/254 of 2dubA
- active site: A67 (≠ G72), M72 (≠ L77), S82 (≠ V103), G105 (= G126), E108 (≠ G129), P127 (= P148), E128 (= E149), T133 (≠ L154), P135 (= P156), G136 (≠ D157)
- binding octanoyl-coenzyme a: K25 (= K29), A26 (= A30), L27 (= L31), A29 (= A33), A65 (= A70), A67 (≠ G72), D68 (= D73), I69 (≠ V74), K70 (≠ V75), G105 (= G126), E108 (≠ G129), P127 (= P148), E128 (= E149), G136 (≠ D157), A137 (≠ V158)
Sites not aligning to the query:
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
34% identity, 56% coverage: 8:208/358 of query aligns to 2:189/258 of 1mj3A
- active site: A68 (≠ G72), M73 (≠ L77), S83 (≠ Y102), L85 (= L104), G109 (= G126), E112 (≠ G129), P131 (= P148), E132 (= E149), T137 (≠ L154), P139 (= P156), G140 (≠ D157)
- binding hexanoyl-coenzyme a: K26 (= K29), A27 (= A30), L28 (= L31), A30 (= A33), A66 (= A70), G67 (= G71), A68 (≠ G72), D69 (= D73), I70 (≠ V74), G109 (= G126), P131 (= P148), E132 (= E149), L135 (≠ I152), G140 (≠ D157)
Sites not aligning to the query:
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
33% identity, 56% coverage: 12:212/358 of query aligns to 5:199/257 of 6slbAAA
- active site: Q64 (≠ G72), F69 (≠ L77), L80 (≠ I94), N84 (= N105), A108 (≠ G126), S111 (≠ G129), A130 (≠ P148), F131 (≠ E149), L136 (= L154), P138 (= P156), D139 (= D157)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (≠ K66), A62 (= A70), Q64 (≠ G72), D65 (= D73), L66 (≠ V74), Y76 (≠ D90), A108 (≠ G126), F131 (≠ E149), D139 (= D157)
Sites not aligning to the query:
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
29% identity, 59% coverage: 11:222/358 of query aligns to 6:213/261 of 5jbxB
- active site: A67 (≠ G72), R72 (≠ A83), L84 (≠ F98), R88 (≠ Y102), G112 (= G126), E115 (≠ G129), T134 (≠ P148), E135 (= E149), I140 (≠ L154), P142 (= P156), G143 (≠ D157)
- binding coenzyme a: S24 (≠ K29), R25 (≠ A30), R26 (≠ L31), A28 (= A33), A65 (= A70), D68 (= D73), L69 (≠ V74), K70 (≠ G81), L110 (≠ M124), G111 (= G125), T134 (≠ P148), E135 (= E149), L138 (≠ I152), R168 (= R181)
Sites not aligning to the query:
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
32% identity, 56% coverage: 12:212/358 of query aligns to 2:187/245 of 6slaAAA
- active site: Q61 (≠ G72), L68 (≠ I94), N72 (= N105), A96 (≠ G126), S99 (≠ G129), A118 (≠ P148), F119 (≠ E149), L124 (= L154), P126 (= P156), N127 (≠ D157)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (= L31), A59 (= A70), Q61 (≠ G72), D62 (= D73), L63 (≠ V74), L68 (≠ I94), Y71 (≠ F97), A94 (≠ M124), G95 (= G125), A96 (≠ G126), F119 (≠ E149), I122 (= I152), L124 (= L154), N127 (≠ D157)
Sites not aligning to the query:
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
29% identity, 54% coverage: 9:202/358 of query aligns to 2:180/250 of 3q0gD
- active site: A64 (≠ G72), M69 (≠ L77), T75 (≠ F98), F79 (≠ Y102), G103 (= G126), E106 (≠ G129), P125 (= P148), E126 (= E149), V131 (≠ L154), P133 (= P156), G134 (≠ D157)
Sites not aligning to the query:
O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
31% identity, 58% coverage: 13:220/358 of query aligns to 11:212/266 of O53561
- K135 (≠ M144) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
- 135:142 (vs. 144:151, 25% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
- K142 (≠ G151) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
29% identity, 55% coverage: 7:202/358 of query aligns to 1:185/255 of 3q0jC
- active site: A65 (≠ G72), M70 (≠ L77), T80 (≠ F98), F84 (≠ Y102), G108 (= G126), E111 (≠ G129), P130 (= P148), E131 (= E149), V136 (≠ L154), P138 (= P156), G139 (≠ D157)
- binding acetoacetyl-coenzyme a: Q23 (≠ K29), A24 (= A30), L25 (= L31), A27 (= A33), A63 (= A70), G64 (= G71), A65 (≠ G72), D66 (= D73), I67 (≠ V74), K68 (≠ V75), M70 (≠ L77), F84 (≠ Y102), G107 (= G125), G108 (= G126), E111 (≠ G129), P130 (= P148), E131 (= E149), P138 (= P156), G139 (≠ D157), M140 (≠ V158)
Sites not aligning to the query:
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
29% identity, 55% coverage: 7:202/358 of query aligns to 1:185/255 of 3q0gC
- active site: A65 (≠ G72), M70 (≠ L77), T80 (≠ F98), F84 (≠ Y102), G108 (= G126), E111 (≠ G129), P130 (= P148), E131 (= E149), V136 (≠ L154), P138 (= P156), G139 (≠ D157)
- binding coenzyme a: L25 (= L31), A63 (= A70), I67 (≠ V74), K68 (≠ V75), Y104 (≠ I122), P130 (= P148), E131 (= E149), L134 (≠ I152)
Sites not aligning to the query:
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
29% identity, 54% coverage: 9:202/358 of query aligns to 2:184/256 of 3h81A
- active site: A64 (≠ G72), M69 (≠ L77), T79 (≠ F98), F83 (≠ Y102), G107 (= G126), E110 (≠ G129), P129 (= P148), E130 (= E149), V135 (≠ L154), P137 (= P156), G138 (≠ D157)
Sites not aligning to the query:
Q7CQ56 1,4-dihydroxy-2-naphthoyl-CoA synthase; DHNA-CoA synthase; EC 4.1.3.36 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
28% identity, 55% coverage: 9:204/358 of query aligns to 22:212/285 of Q7CQ56
4i42A E.Coli. 1,4-dihydroxy-2-naphthoyl coenzyme a synthase (ecmenb) in complex with 1-hydroxy-2-naphthoyl-coa (see paper)
28% identity, 55% coverage: 9:204/358 of query aligns to 22:212/285 of 4i42A
- active site: G86 (= G72), R91 (≠ K82), Y97 (≠ Q88), H105 (≠ A96), L109 (≠ E100), G133 (= G126), V136 (≠ G129), G156 (≠ E149), S161 (≠ L154), D163 (≠ P156), G164 (≠ D157)
- binding 1-hydroxy-2-naphthoyl-CoA: V44 (≠ A30), R45 (≠ L31), S84 (≠ A70), G85 (= G71), G86 (= G72), D87 (= D73), Q88 (≠ E79), K89 (≠ T80), Y97 (≠ Q88), V108 (≠ S99), Y129 (≠ I122), G133 (= G126), T155 (≠ P148), S161 (≠ L154)
Sites not aligning to the query:
Query Sequence
>AZOBR_RS08460 FitnessBrowser__azobra:AZOBR_RS08460
MSDPAEIQFAEILFERRGSIGLVTLNRPKALNALTLGMIRLFDPQLRAWIADPEVKAIVV
QGAGEKAFCAGGDVVNLYETGKAAKAGQDDGASIRAFFSEEYVLNRLIHTCPKPYVALID
GISMGGGVGLSVHGSHRIVTERTMFAMPETGIGLYPDVGGTYFLPRLPGQVGIWLGLTGD
RLKAADMIEVGAADAFVPSAKLESLIAELADGVPADEAVARHREEAGEPPVAANRAAIDR
CYAHNEVEAIVAALEAEGTEWAAGQLATLKRVSPTSLKVTLEALRRGAKLDFDGCMIQEL
RLSLAFLARHDVYEGIRAALVDKDRNPRWSPASLADVTAEEVAGYFAEPVGGDLRFTE
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory