SitesBLAST

C26 (≠ T32) mutation to A: Lower ATPase activity and transport efficiency.
F27 (= F33) mutation to L: Lower ATPase activity and transport efficiency.
F45 (= F51) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ S60) mutation to T: Loss of ATPase activity and transport.
L60 (= L66) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ I82) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V141) mutation to M: Loss of ATPase activity and transport.
D172 (= D178) mutation to N: Loss of ATPase activity and transport.
C276 (= C281) mutation to A: Lower ATPase activity and transport efficiency.
E297 (= E304) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
45% identity, 73% coverage: 24:306/387 of query aligns to 4:288/369 of P19566

query
sites
P19566

V

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Q

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L

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R

K

N

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V

T

T

K

K

T

A

F

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D

F

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G

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G

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M

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F

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Q

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A

A

L
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L

F

Y

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P

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H

M

L

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S

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N

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A

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M

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F

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A

A

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F

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-

K

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M

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M

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A

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L

L86 (= L106) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P180) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D185) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
52% identity, 63% coverage: 24:267/387 of query aligns to 7:258/375 of 2d62A

query
sites
2d62A

V

V

R

K

I

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E

I

K

N

V

I

T

W

K

K

T

R

F

F

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D

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A

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D

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E

D

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A

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x
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S
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S

G
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G

S
x
C

G
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G

K
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T
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T

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M

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A

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D

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Y

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E

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D

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A

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F

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A

A

L

L

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P

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M

M

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T

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E

Y

Q

D

N

N

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A

A

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K

Q

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K

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A

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Q

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K

Q

L

L

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T

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Y

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T

T

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H

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D

Q

Q

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V

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A

A

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I

A

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F

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D

D

binding pyrophosphate 2-: P40 (≠ G57), S41 (= S58), G42 (= G59), C43 (≠ S60), G44 (= G61), K45 (= K62), T46 (= T63), T47 (= T64)

1g291 Malk (see paper)
52% identity, 63% coverage: 24:267/387 of query aligns to 4:255/372 of 1g291

query
sites
1g291

V

V

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R

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D

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K

K

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F

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A

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M

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x
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S

G
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G

S
x
C

G
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G

K
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K

T
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T

L

T

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L

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M

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A

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x
D

-

P

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E

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x
K

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G

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-

F

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x
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D

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A

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F

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D

N

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A

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K

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L

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K

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T

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Y

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V

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T

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H

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D

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Q

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A

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M

M

T

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M

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N

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V

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E

E

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Y
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Y

E
x
D

Y

K

P

P

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A

S

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T

F

F

V

V

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A

E

G

F

F

I

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S

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P

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M

N

N

M

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E

D

D

binding magnesium ion: D69 (≠ G89), E71 (vs. gap), K72 (vs. gap), K79 (≠ Y93), D80 (≠ E94), Y228 (= Y242), D229 (≠ E243)
binding pyrophosphate 2-: P37 (≠ G57), S38 (= S58), G39 (= G59), C40 (≠ S60), G41 (= G61), K42 (= K62), T43 (= T63), T44 (= T64)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 341, 342, 359

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
50% identity, 61% coverage: 24:259/387 of query aligns to 3:238/374 of 2awnB

query
sites
2awnB

V

V

R

Q

I

L

E

Q

K

N

V

V

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T

K

K

T

A

F
x
W

G

G

D

E

F

V

V

V

A
x
V

V

S

D

K

E

D

V

I

S

N

L

L

S

D

I

I

Y

H

R

E

G

G

E

E

F

F

F

V

A

V

L

F

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G

G

G

P

S
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S

G
|
G

S
x
C

G
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G

K
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K

T
x
S

T
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T

L

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R

R

M

M

L

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A

A

G

G

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F

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M

M

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D

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P

Y

A

E

E

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G

V

V

N

G

M

M

M

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

E

A

Q

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

Q

L

D

A

G

G

V

A

A

K

K

K

A

E

E

V

I

I

K

N

E

Q

R

R

V

V

G

N

A

Q

I

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L

A

D

E

L

V

V

L

Q

Q

L

L

G

A

Q

H

F

L

G

L

K

D

R

R

K

K

P

P

H

K

Q

A

L

L

S

S

G

G

Q

Q

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R

Q

Q

R

R

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V

A

A

L

I

A

G

R

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T

L

L

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V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

K

A

R

A

L

L

R

R

E

V

R

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T

M

Q

R

F

I

E

E

L

I

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S

N

R

I

L

Q

H

E

K

K

R

L

L

G

G

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T

F

M

I

I

V

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

S

A

S

D

R

K

I

I

A

V

V

V

M

L

N

D

H

A

G

G

V

R

I

V

A

A

Q

Q

T

V

G

G

T

K

P

P

T

L

E

E

I

L

Y

Y

E

H

Y

Y

P

P

Q

A

S

D

R

R

F

F

V

V

A

A

E

G

F

F

I

I

G

G

S

S

V

P

N

K

M

M

binding adenosine-5'-diphosphate: W12 (≠ F33), V17 (≠ A38), S37 (= S58), G38 (= G59), C39 (≠ S60), G40 (= G61), K41 (= K62), S42 (≠ T63), T43 (= T64)

2awoA Crystal structure of the adp-mg-bound e. Coli malk (crystallized with adp-mg) (see paper)
50% identity, 61% coverage: 24:259/387 of query aligns to 3:238/372 of 2awoA

query
sites
2awoA

V

V

R

Q

I

L

E

Q

K

N

V

V

T

T

K

K

T

A

F
x
W

G

G

D

E

F

V

V

V

A
x
V

V

S

D

K

E

D

V

I

S

N

L

L

S

D

I

I

Y

H

R

E

G

G

E

E

F

F

F

V

A

V

L

F

L

V

G

G

G

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

L

I

A

A

G

G

F

L

E

E

T

T

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I

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S

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G

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D

I

L

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F

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M

A

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P

Y

A

E

E

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R

P

G

V

V

N

G

M

M

M

V

F

F

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Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

E

A

Q

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

Q

L

D

A

G

G

V

A

A

K

K

K

A

E

E

V

I

I

K

N

E

Q

R

R

V

V

G

N

A

Q

I

V

L

A

D

E

L

V

V

L

Q

Q

L

L

G

A

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H

F

L

G

L

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D

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R

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K

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P

H

K

Q

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

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V

A

A

L

I

A

G

R

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T

L

L

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V

K

A

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E

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P

K

S

L

V

L

F

L

L

D
|
D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

K

A

R

A

L

L

R

R

E

V

R

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T

M

Q

R

F

I

E

E

L

I

V

S

N

R

I

L

Q

H

E

K

K

R

L

L

G

G

V

R

T

T

F

M

I

I

V

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

S

A

S

D

R

K

I

I

A

V

V

V

M

L

N

D

H

A

G

G

V

R

I

V

A

A

Q

Q

T

V

G

G

T

K

P

P

T

L

E

E

I

L

Y

Y

E

H

Y

Y

P

P

Q

A

S

D

R

R

F

F

V

V

A

A

E

G

F

F

I

I

G

G

S

S

V

P

N

K

M

M

binding adenosine-5'-diphosphate: W12 (≠ F33), V17 (≠ A38), S37 (= S58), G38 (= G59), C39 (≠ S60), G40 (= G61), K41 (= K62), S42 (≠ T63), T43 (= T64)
binding magnesium ion: S42 (≠ T63), D157 (= D178)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
50% identity, 61% coverage: 24:259/387 of query aligns to 4:239/371 of P68187

query
sites
P68187

V

V

R

Q

I

L

E

Q

K

N

V

V

T

T

K

K

T

A

F

W

G

G

D

E

F

V

V

V

A

V

V

S

D

K

E

D

V

I

S

N

L

L

S

D

I

I

Y

H

R

E

G

G

E

E

F

F

F

V

A

V

L

F

L

V

G

G

G

P

S

S

G

G

S

C

G

G

K

K

T

S

T

T

L

L

R

R

M

M

L

I

A

A

G

G

F

L

E

E

T

T

P

I

T

T

E

S

G

G

K

D

I

L

F

F

I

I

D

G

G

E

V

K

D

R

M

M

A

N

G

D

I

T

P

P

Y

A

E

E

R

R

P

G

V

V

N

G

M

M

M

V

F

F

Q

Q

S

S

Y

Y

A
|
A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

E

A

Q

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

Q

L

D

A

G

G

V

A

A
x
K

K

K

A

E

E

V

I

I

K

N

E

Q

R

R

V
|
V

G

N

A

Q

I
x
V

L

A

D
x
E

L

V

V

L

Q

Q

L

L

G
x
A

Q

H

F

L

G

L

K

D

R

R

K

K

P

P

H

K

Q

A

L

L

S

S

G

G

G
|
G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

V

V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D
|
D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

K

A

R

A

L

L

R

R

E

V

R

Q

T

M

Q

R

F

I

E

E

L

I

V

S

N

R

I

L

Q

H

E

K

K

R

L

L

G

G

V

R

T

T

F

M

I

I

V

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

S

A

S

D

R

K

I

I

A

V

V

V

M

L

N

D

H

A

G

G

V

R

I

V

A

A

Q

Q

T

V

G

G

T

K

P

P

T

L

E

E

I

L

Y

Y

E

H

Y

Y

P

P

Q

A

S

D

R
|
R

F

F

V

V

A

A

E

G

F

F

I

I

G

G

S

S

V

P

N

K

M

M

A85 (= A105) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ A126) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V134) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ I137) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ D139) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ G144) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G157) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D178) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (= R248) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
50% identity, 61% coverage: 24:259/387 of query aligns to 3:238/371 of 3puyA

query
sites
3puyA

V

V

R

Q

I

L

E

Q

K

N

V

V

T

T

K

K

T

A

F
x
W

G

G

D

E

F

V

V

V

A
x
V

V

S

D

K

E

D

V

I

S

N

L

L

S

D

I

I

Y

H

R

E

G

G

E

E

F

F

F

V

A

V

L

F

L

V

G

G

G
x
P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

L

I

A

A

G

G

F

L

E

E

T

T

P

I

T

T

E

S

G

G

K

D

I

L

F

F

I

I

D

G

G

E

V

K

D

R

M

M

A

N

G

D

I

T

P

P

Y

A

E

E

R

R

P

G

V

V

N

G

M

M

M

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

E

A

Q

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

Q

L

D

A

G

G

V

A

A

K

K

K

A

E

E

V

I

I

K

N

E

Q

R

R

V

V

G

N

A

Q

I

V

L

A

D

E

L

V

V

L

Q

Q

L

L

G

A

Q

H

F

L

G

L

K

D

R
|
R

K

K

P

P

H

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

V

V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D
|
D

E
|
E

P

P

L

L

G

S

A

N

L

L

D

D

K

A

R

A

L

L

R

R

E

V

R

Q

T

M

Q

R

F

I

E

E

L

I

V

S

N

R

I

L

Q

H

E

K

K

R

L

L

G

G

V

R

T

T

F

M

I

I

V

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

S

A

S

D

R

K

I

I

A

V

V

V

M

L

N

D

H

A

G

G

V

R

I

V

A

A

Q

Q

T

V

G

G

T

K

P

P

T

L

E

E

I

L

Y

Y

E

H

Y

Y

P

P

Q

A

S

D

R

R

F

F

V

V

A

A

E

G

F

F

I

I

G

G

S

S

V

P

N

K

M

M

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F33), V17 (≠ A38), P36 (≠ G57), S37 (= S58), G38 (= G59), C39 (≠ S60), G40 (= G61), K41 (= K62), S42 (≠ T63), T43 (= T64), Q81 (= Q102), R128 (= R149), A132 (≠ Q153), S134 (= S155), G135 (= G156), G136 (= G157), Q137 (= Q158), E158 (= E179), H191 (= H212)
binding magnesium ion: S42 (≠ T63), Q81 (= Q102), D157 (= D178)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
50% identity, 61% coverage: 24:259/387 of query aligns to 3:238/371 of 3puxA

query
sites
3puxA

V

V

R

Q

I

L

E

Q

K

N

V

V

T

T

K

K

T

A

F
x
W

G

G

D

E

F

V

V

V

A
x
V

V

S

D

K

E

D

V

I

S

N

L

L

S

D

I

I

Y

H

R

E

G

G

E

E

F

F

F

V

A

V

L

F

L

V

G

G

G

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

L

I

A

A

G

G

F

L

E

E

T

T

P

I

T

T

E

S

G

G

K

D

I

L

F

F

I

I

D

G

G

E

V

K

D

R

M

M

A

N

G

D

I

T

P

P

Y

A

E

E

R

R

P

G

V

V

N

G

M

M

M

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

E

A

Q

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

Q

L

D

A

G

G

V

A

A

K

K

K

A

E

E

V

I

I

K

N

E

Q

R

R

V

V

G

N

A

Q

I

V

L

A

D

E

L

V

V

L

Q

Q

L

L

G

A

Q

H

F

L

G

L

K

D

R
|
R

K

K

P

P

H

K

Q
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

V

V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D
|
D

E
|
E

P

P

L

L

G

S

A

N

L

L

D

D

K

A

R

A

L

L

R

R

E

V

R

Q

T

M

Q

R

F

I

E

E

L

I

V

S

N

R

I

L

Q

H

E

K

K

R

L

L

G

G

V

R

T

T

F

M

I

I

V

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

S

A

S

D

R

K

I

I

A

V

V

V

M

L

N

D

H

A

G

G

V

R

I

V

A

A

Q

Q

T

V

G

G

T

K

P

P

T

L

E

E

I

L

Y

Y

E

H

Y

Y

P

P

Q

A

S

D

R

R

F

F

V

V

A

A

E

G

F

F

I

I

G

G

S

S

V

P

N

K

M

M

binding adenosine-5'-diphosphate: W12 (≠ F33), V17 (≠ A38), S37 (= S58), G38 (= G59), C39 (≠ S60), G40 (= G61), K41 (= K62), S42 (≠ T63), T43 (= T64), R128 (= R149), A132 (≠ Q153), L133 (= L154), S134 (= S155), Q137 (= Q158)
binding beryllium trifluoride ion: S37 (= S58), G38 (= G59), K41 (= K62), Q81 (= Q102), S134 (= S155), G135 (= G156), G136 (= G157), E158 (= E179), H191 (= H212)
binding magnesium ion: S42 (≠ T63), Q81 (= Q102), D157 (= D178)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
50% identity, 61% coverage: 24:259/387 of query aligns to 3:238/371 of 3puwA

query
sites
3puwA

V

V

R

Q

I

L

E

Q

K

N

V

V

T

T

K

K

T

A

F
x
W

G

G

D

E

F

V

V

V

A
x
V

V

S

D

K

E

D

V

I

S

N

L

L

S

D

I

I

Y

H

R

E

G

G

E

E

F

F

F

V

A

V

L

F

L

V

G

G

G

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

L

I

A

A

G

G

F

L

E

E

T

T

P

I

T

T

E

S

G

G

K

D

I

L

F

F

I

I

D

G

G

E

V

K

D

R

M

M

A

N

G

D

I

T

P

P

Y

A

E

E

R

R

P

G

V

V

N

G

M

M

M

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

E

A

Q

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

Q

L

D

A

G

G

V

A

A

K

K

K

A

E

E

V

I

I

K

N

E

Q

R

R

V

V

G

N

A

Q

I

V

L

A

D

E

L

V

V

L

Q

Q

L

L

G

A

Q

H

F

L

G

L

K

D

R
|
R

K

K

P

P

H

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

V

V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D

D

E
|
E

P

P

L

L

G

S

A
x
N

L

L

D

D

K

A

R

A

L

L

R

R

E

V

R

Q

T

M

Q

R

F

I

E

E

L

I

V

S

N

R

I

L

Q

H

E

K

K

R

L

L

G

G

V

R

T

T

F

M

I

I

V

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

S

A

S

D

R

K

I

I

A

V

V

V

M

L

N

D

H

A

G

G

V

R

I

V

A

A

Q

Q

T

V

G

G

T

K

P

P

T

L

E

E

I

L

Y

Y

E

H

Y

Y

P

P

Q

A

S

D

R

R

F

F

V

V

A

A

E

G

F

F

I

I

G

G

S

S

V

P

N

K

M

M

binding adenosine-5'-diphosphate: W12 (≠ F33), V17 (≠ A38), S37 (= S58), G38 (= G59), C39 (≠ S60), G40 (= G61), K41 (= K62), S42 (≠ T63), T43 (= T64), R128 (= R149), A132 (≠ Q153), S134 (= S155), Q137 (= Q158)
binding tetrafluoroaluminate ion: S37 (= S58), G38 (= G59), K41 (= K62), Q81 (= Q102), S134 (= S155), G135 (= G156), G136 (= G157), E158 (= E179), N162 (≠ A183), H191 (= H212)
binding magnesium ion: S42 (≠ T63), Q81 (= Q102)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
50% identity, 61% coverage: 24:259/387 of query aligns to 1:236/367 of 1q12A

query
sites
1q12A

V

V

R

Q

I

L

E

Q

K

N

V

V

T

T

K

K

T

A

F
x
W

G

G

D

E

F

V

V

V

A
x
V

V

S

D

K

E

D

V

I

S

N

L

L

S

D

I

I

Y

H

R

E

G

G

E

E

F

F

F

V

A

V

L

F

L

V

G

G

G

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

L

I

A

A

G

G

F

L

E

E

T

T

P

I

T

T

E

S

G

G

K

D

I

L

F

F

I

I

D

G

G

E

V

K

D

R

M

M

A

N

G

D

I

T

P

P

Y

A

E

E

R

R

P

G

V

V

N

G

M

M

M

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

S

S

V

V

E

A

Q

E

N

N

V

M

A

S

F

F

G

G

L

L

K

K

Q

L

D

A

G

G

V

A

A

K

K

K

A

E

E

V

I

I

K

N

E

Q

R

R

V

V

G

N

A

Q

I

V

L

A

D

E

L

V

V

L

Q

Q

L

L

G

A

Q

H

F

L

G

L

K

D

R
|
R

K

K

P

P

H
x
K

Q
x
A

L
|
L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

S

T

L

L

V

V

K

A

R

E

P

P

K

S

L

V

L

F

L

L

D

D

E

E

P

P

L

L

G

S

A
x
N

L

L

D

D

K

A

R

A

L

L

R

R

E

V

R

Q

T

M

Q

R

F

I

E

E

L

I

V

S

N

R

I

L

Q

H

E

K

K

R

L

L

G

G

V

R

T

T

F

M

I

I

V

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

L

S

A

S

D

R

K

I

I

A

V

V

V

M

L

N

D

H

A

G

G

V

R

I

V

A

A

Q

Q

T

V

G

G

T

K

P

P

T

L

E

E

I

L

Y

Y

E

H

Y

Y

P

P

Q

A

S

D

R

R

F

F

V

V

A

A

E

G

F

F

I

I

G

G

S

S

V

P

N

K

M

M

binding adenosine-5'-triphosphate: W10 (≠ F33), V15 (≠ A38), S35 (= S58), G36 (= G59), C37 (≠ S60), G38 (= G61), K39 (= K62), S40 (≠ T63), T41 (= T64), R126 (= R149), K129 (≠ H152), A130 (≠ Q153), L131 (= L154), S132 (= S155), G133 (= G156), G134 (= G157), Q135 (= Q158), N160 (≠ A183), H189 (= H212)

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
47% identity, 74% coverage: 24:309/387 of query aligns to 7:281/353 of 1vciA

query
sites
1vciA

V

V

R

K

I

L

E

E

K

N

V

L

T

T

K

K

T

R

F
|
F

G

G

D

N

F
|
F

V

T

A

A

V

V

D

N

E

K

V

L

S

N

L

L

S

T

I

I

Y

K

R

D

G

G

E

E

F

F

F

L

A

V

L

L

G

G

G
x
P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
|
T

L

T

L

L

R

R

M

M

L

I

A

A

G

G

F

L

E

E

T

E

P

P

T

T

E

E

G

G

K

R

I

I

F

Y

I

F

D

G

G

D

V

R

D

D

M

V

A

T

G

Y

I

L

P

P

Y

K

E

D

R

R

P

N

V

I

N

S

M

M

M

V

F

F

Q

Q

S

-

Y

-

A

-

L

-

F

-

P

-

H

H

M

M

S

T

V

V

E

Y

Q

E

N

N

V

I

A

A

F

F

G

P

L

L

K

K

Q

K

D

-

G

-

V

F

A

P

K

K

A

D

E

E

I

I

K

D

E

K

R

R

V

V

G

R

A

W

I

A

L

A

D

E

L

L

V

L

Q

Q

L

I

G

E

Q

E

F

L

G

L

K

N

R

R

K

Y

P

P

H

A

Q

Q

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

V

A

A

R

R

S

A

L

I

V

V

K

V

R

E

P

P

K

D

L

V

L

L

L

M

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

K

A

R

K

L

L

R

R

E

V

R

A

T

M

Q

R

F

A

E

E

L

I

V

K

N

K

I

L

Q

Q

E

Q

K

K

L

L

G

K

V

V

T

T

F

T

I

I

V

Y

V

V

T

T

H

H

D

D

Q

Q

E

V

E

E

A

A

M

M

T

T

M

M

S

G

S

D

R

R

I

I

A

A

V

V

M

M

N

N

H

R

G

G

V

Q

I

L

A

L

Q

Q

T

I

G

G

T

S

P

P

T

T

E

E

I

V

Y

Y

E

L

Y

R

P

P

Q

N

S

S

R

V

F

F

V

V

A

A

E

T

F

F

I

I

G

G

S

A

-

P

-

E

V

M

N

N

M

I

F

L

E

E

G

V

R

S

V

V

V

G

E

D

D

G

Q

Y

A

L

D

E

H

G

V

R

L

G

I

F

R

R

S

I

E

E

D

L

A

P

G

Q

C

M

D

D

L

L

Y

L

I

K

N

D

H

Y

A

-

V

-

A

-

V

-

P

-

A

V

G

G

A

K

T

T

V

V

G

L

V

F

A

G

V

I

R

R

P

P

E

E

K

H

I

M

A

T

L

V

S

E

binding adenosine-5'-triphosphate: F16 (= F33), F19 (= F36), P40 (≠ G57), S41 (= S58), G42 (= G59), C43 (≠ S60), G44 (= G61), K45 (= K62), T46 (= T63), T47 (= T64)

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
46% identity, 62% coverage: 26:264/387 of query aligns to 6:247/393 of P9WQI3

query
sites
P9WQI3

I

L

E

D

K

H

V

V

T

N

K

K

T

S

F

Y

G

P

D

D

-

G

F

H

V

T

A

A

V

V

D

R

E

D

V

L

S

N

L

L

S

T

I

I

Y

A

R

D

G

G

E

E

F

F

F

L

A

I

L

L

L

V

G

G

G

P

S

S

G

G

S

C

G

G

K

K

T

T

L

T

L

L

R

N

M

M

L

I

A

A

G

G

F

L

E

E

T

D

P

I

T

S

E

S

G

G

K

E

I

L

F

R

I

I

D

A

G

G

V

E

D

R

M

V

A

N

G

E

I

K

P

A

P

P

Y

K

E

D

R

R

P

D

V

I

N

A

M

M

M

V

F

F

Q

Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

S

T

V

V

E

R

Q

Q

N

N

V

I

A

A

F

F

G

P

L

L

K

T

Q

L

D

A

G

K

V

M

A

R

K

K

A

A

E

D

I

I

K

A

E

Q

R

K

V

V

G

S

A

E

I

T

L

A

D

K

L

I

V

L

Q

D

L

L

G

T

Q

N

F

L

G

L

K

D

R

R

K

K

P

P

H

S

Q

Q

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

S

A

L

I

V

V

K

R

R

H

P

P

K

K

L

A

L

F

L

L

L

M

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

K

A

R

K

L

L

R

R

E

V

R

Q

T

M

Q

R

F

G

E

E

L

I

V

A

N

Q

I

L

Q

Q

E

R

K

R

L

L

G

G

V

T

T

T

F

T

I

V

V

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

T

E

E

A

A

M

M

T

T

M

L

S

G

S

D

R

R

I

V

A

V

V

V

M

M

N

Y

H

G

G

G

V

I

I

A

A

Q

Q

Q

T

I

G

G

T

T

P

P

T

E

E

E

I

L

Y

Y

E

E

Y

R

P

P

Q

A

S

N

R

L

F

F

V

V

A

A

E

G

F

F

I

I

G

G

S

S

-

P

-

A

V

M

N

N

M

F

F

F

E

P

G

A

R

R

V

L

H193 (= H212) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

8hprD ABC transporter, ATP-binding protein SugC (see paper)
47% identity, 61% coverage: 26:260/387 of query aligns to 5:242/362 of 8hprD

query
sites
8hprD

I

L

E

D

K

R

V

V

T

T

K

K

T

S

F
x
Y

G

P

D

D

F

V

-

R

V

A

A

A

V

V

D

K

E

E

V

F

S

S

L

M

S

T

I

I

Y

A

R

D

G

G

E

E

F

F

F

I

A

I

L

L

L

V

G

G

G

P

S
|
S

G

G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

T

L

L

R

N

M

M

L

I

A

A

G

G

F

L

E

E

T

E

P

I

T

T

E

S

G

G

K

E

I

L

F

R

I

I

D

G

G

G

V

E

D

R

M

V

A

N

G

E

I

K

P

A

P

P

Y

K

E

D

R

R

P

D

V

I

N

A

M

M

M

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

S

T

V

V

E

R

Q

Q

N

N

V

I

A

A

F

F

G

P

L

L

K

T

Q

L

D

A

G

K

V

V

A

P

K

K

A

A

E

E

I

I

K

A

E

A

R

K

V

V

G

E

A

E

I

T

L

A

D

K

L

I

V

L

Q

D

L

L

G

S

Q

E

F

L

G

L

K

D

R
|
R

K

K

P

P

H
x
G

Q
|
Q

L

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

S

A

L

I

V

V

K

R

R

S

P

P

K

K

L

A

L

F

L

L

L

M

D
|
D

E
x
Q

P

P

L

L

G

S

A

N

L

L

D

D

K

A

R

K

L

L

R

R

E

V

R

Q

T

M

Q

R

F

A

E

E

L

I

V

S

N

R

I

L

Q

Q

E

D

K

R

L

L

G

G

V

T

T

T

F

T

I

V

V

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

T

E

E

A

A

M

M

T

T

M

L

S

G

S

D

R

R

I

V

A

V

V

V

M

M

N

L

H

A

G

G

V

E

I

V

A

Q

Q

Q

T

I

G

G

T

T

P

P

T

D

E

E

I

L

Y

Y

E

S

Y

S

P

P

Q

A

S

N

R

L

F

F

V

V

A

A

E

G

F

F

I

I

G

G

S

S

-

P

-

A

V

M

N

N

M

F

F

F

binding adenosine-5'-triphosphate: Y12 (≠ F33), S38 (= S58), C40 (≠ S60), G41 (= G61), K42 (= K62), S43 (≠ T63), T44 (= T64), Q82 (= Q102), R129 (= R149), G132 (≠ H152), Q133 (= Q153), S135 (= S155), G136 (= G156), G137 (= G157), Q138 (= Q158), Q159 (≠ E179), H192 (= H212)
binding magnesium ion: S43 (≠ T63), Q82 (= Q102), D158 (= D178)

8hprC ABC transporter, ATP-binding protein SugC (see paper)
47% identity, 61% coverage: 26:260/387 of query aligns to 5:242/363 of 8hprC

query
sites
8hprC

I

L

E

D

K

R

V

V

T

T

K

K

T

S

F
x
Y

G

P

D

D

F

V

-

R

V

A

A

A

V

V

D

K

E

E

V

F

S

S

L

M

S

T

I

I

Y

A

R

D

G

G

E

E

F

F

F

I

A

I

L

L

L

V

G

G

G

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

T

L

L

R

N

M

M

L

I

A

A

G

G

F

L

E

E

T

E

P

I

T

T

E

S

G

G

K

E

I

L

F

R

I

I

D

G

G

G

V

E

D

R

M

V

A

N

G

E

I

K

P

A

P

P

Y

K

E

D

R

R

P

D

V

I

N

A

M

M

M

V

F

F

Q
|
Q

S

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

S

T

V

V

E

R

Q

Q

N

N

V

I

A

A

F

F

G

P

L

L

K

T

Q

L

D

A

G

K

V

V

A

P

K

K

A

A

E

E

I

I

K

A

E

A

R

K

V

V

G

E

A

E

I

T

L

A

D

K

L

I

V

L

Q

D

L

L

G

S

Q

E

F

L

G

L

K

D

R

R

K

K

P

P

H
x
G

Q
|
Q

L
|
L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

S

A

L

I

V

V

K

R

R

S

P

P

K

K

L

A

L

F

L

L

L

M

D

D

E

Q

P

P

L

L

G

S

A

N

L

L

D

D

K

A

R

K

L

L

R

R

E

V

R

Q

T

M

Q

R

F

A

E

E

L

I

V

S

N

R

I

L

Q

Q

E

D

K

R

L

L

G

G

V

T

T

T

F

T

I

V

V

Y

V

V

T

T

H
|
H

D

D

Q

Q

E

T

E

E

A

A

M

M

T

T

M

L

S

G

S

D

R

R

I

V

A

V

V

V

M

M

N

L

H

A

G

G

V

E

I

V

A

Q

Q

Q

T

I

G

G

T

T

P

P

T

D

E

E

I

L

Y

Y

E

S

Y

S

P

P

Q

A

S

N

R

L

F

F

V

V

A

A

E

G

F

F

I

I

G

G

S

S

-

P

-

A

V

M

N

N

M

F

F

F

binding adenosine-5'-triphosphate: Y12 (≠ F33), S38 (= S58), G39 (= G59), C40 (≠ S60), G41 (= G61), K42 (= K62), S43 (≠ T63), T44 (= T64), Q82 (= Q102), G132 (≠ H152), Q133 (= Q153), L134 (= L154), S135 (= S155), G136 (= G156), G137 (= G157), Q138 (= Q158), H192 (= H212)
binding magnesium ion: S43 (≠ T63), Q82 (= Q102)

8hplC ABC transporter, ATP-binding protein SugC (see paper)
47% identity, 61% coverage: 26:260/387 of query aligns to 5:240/384 of 8hplC

query
sites
8hplC

I

L

E

D

K

R

V

V

T

T

K

K

T

S

F
x
Y

G

P

D

R

F

-

V

A

A
|
A

V

V

D

K

E

E

V

F

S

S

L

M

S

T

I

I

Y

A

R

D

G

G

E

E

F

F

F

I

A

I

L

L

L

V

G

G

G

P

S
|
S

G
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C

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binding adenosine-5'-triphosphate: Y12 (≠ F33), A16 (= A38), S36 (= S58), G37 (= G59), C38 (≠ S60), G39 (= G61), K40 (= K62), S41 (≠ T63), T42 (= T64)

3d31A Modbc from methanosarcina acetivorans (see paper)
39% identity, 81% coverage: 24:337/387 of query aligns to 2:302/348 of 3d31A

query
sites
3d31A

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