SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AZOBR_RS09720 FitnessBrowser__azobra:AZOBR_RS09720 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
71% identity, 97% coverage: 12:493/497 of query aligns to 1:481/482 of P25526

query
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P25526

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WN 156:157 (= WN 167:168) binding
KPAS 180:183 (≠ KPAT 191:194) binding
GS 233:234 (= GS 244:245) binding
L256 (= L267) binding
E386 (= E398) binding

3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
71% identity, 97% coverage: 14:493/497 of query aligns to 2:480/481 of 3jz4A

query
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3jz4A

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active site: N156 (= N168), K179 (= K191), E254 (= E266), C288 (= C300), E385 (= E398), E462 (= E475)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I152 (= I164), P154 (= P166), W155 (= W167), N156 (= N168), K179 (= K191), A181 (= A193), S182 (≠ T194), S211 (= S223), A212 (= A224), G213 (≠ R225), G216 (= G228), F230 (= F242), T231 (= T243), G232 (= G244), S233 (= S245), I236 (= I248), E254 (= E266), L255 (= L267), C288 (= C300), K338 (= K351), E385 (= E398), F387 (= F400)

P51649 Succinate-semialdehyde dehydrogenase, mitochondrial; Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase; EC 1.2.1.24 from Homo sapiens (Human) (see 5 papers)
57% identity, 98% coverage: 9:495/497 of query aligns to 48:535/535 of P51649

query
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P51649

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A

F

F

A

V

A

K

K

A

L

F

A

A

E

E

A

A

V

M

K

K

A

K

-

N

L

L

K

R

V

V

G

G

P
x
N

G

G

L

F

T

E

T

-

E

E

G

G

A

T

Q

T

Q

Q

G

G

P
|
P

L

L

I

I

D

N

M

E

A

K

A

A

V

V

E

E

K

K

V

V

E

E

D

K

H

Q

I

V

R

N

D

D

A

A

T

V

E

S

K

K

G

G

A

A

R

T

V
|
V

V

V

L

T

G
|
G

G

G

K

K

R

R

H

H

E

Q

L

L

G

G

G

K

S

N

F

F

E

E

P

P

T

T

I

L

L

L

A

C

D

N

V

V

T

T

P

Q

A

D

M

M

K

L

V

C

A

T

R

H

E

E

T

T

F

F

G

G

P

P

V

L

A

A

P

P

L

V

F

I

R

K

F

F

E

D

T

T

E

E

A

A

V

I

R

A

M

I

A

A

N

N

A

A

T

A

E

D

F

V

G

G

L

L

A

A

A

G

Y

Y

F

F

Y

Y

S

S

R

Q

D

D

I

P

G

A

R

Q

V

I

W

W

R

R

V

V

A

A

E

E

A

Q

L

L

E

E

Y

V

G

G

I

M

V

V

G

G

I

V

N

N

E

E

G

G

I

L

I

I

S
|
S

T

S

E

V

V

E

A

C

P

P

F

F

G

G

M

V

K

K

E

Q

S

S

G

G

I

L

G

G

R

R

E

E

G

G

S

S

K

K

Y

Y

G

G

I

I

E

D

D

E

Y

Y

L

L

E

E

I

L

K

K

Y

Y

L

V

C

C

M

Y

G
|
G

G

G

I

L

C93 (≠ M56) to F: in SSADHD; 3% of activity; dbSNP:rs765561257
G176 (= G139) to R: in SSADHD; <1% of activity; dbSNP:rs72552281
H180 (≠ P143) to Y: 83% of activity; dbSNP:rs2760118
P182 (≠ H145) to L: 48% of activity; dbSNP:rs3765310
R213 (= R176) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
C223 (= C186) to Y: in SSADHD; 5% of activity; dbSNP:rs72552282
KPAE 228:231 (≠ KPAT 191:194) binding
T233 (= T196) to M: in SSADHD; 4% of activity; dbSNP:rs1326526453
A237 (= A200) to S: 65% of activity; dbSNP:rs62621664
N255 (≠ S218) to S: in SSADHD; 17% of activity; dbSNP:rs145087265
G268 (= G228) to E: in SSADHD; <1% of activity; dbSNP:rs375628463
GSTTTG 284:289 (≠ GSTEIG 244:249) binding
R334 (= R294) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
N335 (= N295) to K: in SSADHD; 1% of activity; dbSNP:rs72552283
C340 (= C300) modified: Disulfide link with 342, In inhibited form
C342 (= C302) modified: Disulfide link with 340, In inhibited form; mutation to A: Loss of regulation by redox state.
N372 (≠ P331) natural variant: N -> S
P382 (= P342) to L: in SSADHD; 2% of activity
V406 (= V366) to I: in dbSNP:rs143741652
G409 (= G369) to D: in SSADHD; <1% of activity; dbSNP:rs118203984
S498 (= S458) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
G533 (= G493) to R: in SSADHD; <1% of activity; dbSNP:rs72552284

Sites not aligning to the query:

36 G → R: no effect on succinate-semialdehyde dehydrogenase activity; dbSNP:rs4646832

2w8rA The crystal structure of human ssadh in complex with NAD+ (see paper)
57% identity, 97% coverage: 16:495/497 of query aligns to 5:485/485 of 2w8rA

query
sites
2w8rA

D

S

A

A

E

A

L

L

R

R

F

T

Q

D

G

S

F

F

V

V

D

G

G

G

R

R

W

W

I

L

D

P

A

A

D

A

S

A

G

-

K

-

T

T

V

F

E

P

V

V

T

Q

N

D

P

P

A

A

D

S

G

G

S

A

V

A

L

L

G

G

S

M

V

V

P

A

M

D

M

C

G

G

A

V

D

R

E

E

T

A

R

R

R

A

A

A

I

V

E

R

A

A

E

Y

R

E

A

A

W

F

P

C

A

R

W

W

R

R

A

E

L

V

T

S

A

A

K

K

E

E

R

R

A

S

K

S

T

L

L

L

R

R

T

K

W

W

F

Y

D

N

L

L

M

M

M

I

A

Q

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N

Q

K

E

D

D

D

I

L

A

A

R

R

I

I

M

I

T

T

A

A

E

E

Q

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P

L

L

A

K

E

E

A

A

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H

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G

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E

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I

A

L

Y

Y

A

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A

A

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F

F

F

I

L

E

E

W

W

F

F

A

S

E

E

G

A

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R

R

V

V

Y

Y

G

G

D

D

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I

I

I

P

H

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T

H

P

L

A

P

K

G

D

R

R

I

A

V

L

V

V

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L

K

K

E

Q

P

P

I

I

G

G

V

V

T

A

A

A

A

V

I
|
I

T
|
T

P
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P

W
|
W

N
|
N

F

F

P

P

A

S

A

A

M

M

I

I

T

T

R

R

K

K

A

V

G

G

P

A

A

A

L

L

A

A

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G

C

C

P

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M

V

V

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I

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|
K

P
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P

A
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A

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x
E

A

D

T

T

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P

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F

T

S

A

A

L

L

A

A

M

L

A

A

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L

L

A

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E

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R

Q

A

A

G

G

I

I

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P

A

S

G

G

I

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Y

S

N

V

V

V

I

T

P

G

C

S

S

-

R

-

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-

N

A
|
A

R
x
K

A

E

I

V

G

G

G

E

E

A

M

I

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C

G

T

N

D

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P

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F
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F

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T

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G

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S

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T

E

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I
x
T

G

G

K

K

E

I

L

L

M

L

A

H

Q

H

C

A

A

A

G

N

T

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V

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K

K

R

V

V

S

S

L

M

E
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E

L

L

G

G

N

L

A

A

P

P

F

F

L

I

V

V

F

F

N

D

D

S

A

A

D

N

L

V

D

D

E

Q

A

A

V

V

K

A

G

G

A

A

I

M

A

A

S

S

K

K

Y

F

R

R

N

N

T

T

G

G

Q

Q

T

T

C
x
A

V

V

C

C

A

S

N

N

R

Q

L

F

L

L

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G

G

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D

A

A

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F

A

V

A

K

K

A

L

F

A

A

E

E

A

A

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M

K

K

A

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-

N

L

L

K

R

V

V

G

G

P

N

G

G

L

F

T

E

T

-

E

E

G

G

A

T

Q

T

Q

Q

G

G

P

P

L

L

I

I

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N

M

E

A

K

A

A

V

V

E

E

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N

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A

A

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V

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T

G

G

K

K

R

R

H

H

E

Q

L

L

G

G

G

K

S

N

F

F

E

E

P

P

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T

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L

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A

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N

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A

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E

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E
|
E

T

T

F

F

G

G

P

P

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L

A

A

P

P

L

V

F

I

R

K

F

F

E

D

T

T

E

E

A

A

V

I

R

A

M

I

A

A

N

N

A

A

T

A

E

D

F

V

G

G

L

L

A

A

A

G

Y

Y

F

F

Y

Y

S

S

R

Q

D

D

I

P

G

A

R

Q

V

I

W

W

R

R

V

V

A

A

E

E

A

Q

L

L

E

E

Y

V

G

G

I

M

V

V

G

G

I

V

N

N

E

E

G

G

I

L

I

I

S

S

T

S

E

V

V

E

A

C

P

P

F

F

G

G

M

V

K

K

E

Q

S

S

G

G

I

L

G

G

R

R

E
|
E

G

G

S

S

K

K

Y

Y

G

G

I

I

E

D

D

E

Y

Y

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G

G

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L

active site: N155 (= N168), K178 (= K191), E256 (= E266), A290 (≠ C300), E388 (= E398), E465 (= E475)
binding adenosine-5'-diphosphate: I151 (= I164), T152 (= T165), P153 (= P166), W154 (= W167), K178 (= K191), P179 (= P192), A180 (= A193), E181 (≠ T194), A214 (= A224), K215 (≠ R225), F232 (= F242), S235 (= S245), T238 (≠ I248)

2w8qA The crystal structure of human ssadh in complex with ssa. (see paper)
57% identity, 97% coverage: 16:495/497 of query aligns to 5:485/485 of 2w8qA

query
sites
2w8qA

D

S

A

A

E

A

L

L

R

R

F

T

Q

D

G

S

F

F

V

V

D

G

G

G

R

R

W

W

I

L

D

P

A

A

D

A

S

A

G

-

K

-

T

T

V

F

E

P

V

V

T

Q

N

D

P

P

A

A

D

S

G

G

S

A

V

A

L

L

G

G

S

M

V

V

P

A

M

D

M

C

G

G

A

V

D

R

E

E

T

A

R

R

R

A

A

A

I

V

E

R

A

A

E

Y

R

E

A

A

W

F

P

C

A

R

W

W

R

R

A

E

L

V

T

S

A

A

K

K

E

E

R

R

A

S

K

S

T

L

L

L

R

R

T

K

W

W

F

Y

D

N

L

L

M

M

M

I

A

Q

N

N

Q

K

E

D

D

D

I

L

A

A

R

R

I

I

M

I

T

T

A

A

E

E

Q

S

G

G

K

K

P

P

L

L

A

K

E

E

A

A

R

H

G

G

E

E

V

I

A

L

Y
|
Y

A

S

A

A

S

F

F

F

I

L

E

E

W

W

F

F

A

S

E

E

G

A

K

R

R

R

V

V

Y

Y

G

G

D

D

T

I

I

I

P

H

Q

T

H

P

L

A

P

K

G

D

R

R

I

A

V

L

V

V

T

L

K

K

E

Q

P

P

I

I

G

G

V

V

T

A

A

A

A

V

I

I

T

T

P

P

W

W

N
|
N

F
|
F

P

P

A

S

A

A

M

M

I

I

T

T

R
|
R

K

K

A

V

G

G

P

A

A

A

L

L

A

A

G

G

C

C

P

T

M

V

V

V

I

V

K
|
K

P

P

A

A

T

E

A

D

T

T

P

P

L

F

T

S

A

A

L

L

A

A

M

L

A

A

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E

L

L

A

A

E

S

R

Q

A

A

G

G

I

I

P

P

A

S

G

G

I

V

L

Y

S

N

V

V

V

I

T

P

G

C

S

S

-

R

-

K

-

N

A

A

R

K

A

E

I

V

G

G

G

E

E

A

M

I

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C

G

T

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D

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F

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G

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A

A

A

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E
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E

L

L

G

G

N

L

A

A

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P

F

F

L

I

V

V

F

F

N

D

D

S

A

A

D

N

L

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D

D

E

Q

A

A

V

V

K

A

G

G

A

A

I

M

A

A

S

S

K

K

Y

F

R
|
R

N

N

T

T

G

G

Q

Q

T

T

C
x
A

V
|
V

C

C

A

S

N

N

R

Q

L

F

L

L

V

V

Q

Q

S

R

G

G

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I

Y

H

D

D

A

A

F

F

A

V

A

K

K

A

L

F

A

A

E

E

A

A

V

M

K

K

A

K

-

N

L

L

K

R

V

V

G

G

P

N

G

G

L

F

T

E

T

-

E

E

G

G

A

T

Q

T

Q

Q

G

G

P

P

L

L

I

I

D

N

M

E

A

K

A

A

V

V

E

E

K

K

V

V

E

E

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I

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N

D

D

A

A

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K

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G

A

A

R

T

V

V

L

T

G

G

K

K

R

R

H

H

E

Q

L

L

G

G

G

K

S

N

F

F

E

E

P

P

T

T

I

L

L

L

A

C

D

N

V

V

T

T

P

Q

A

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M

M

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V

C

A

T

R

H

E

E

E
|
E

T

T

F

F

G

G

P

P

V

L

A

A

P

P

L

V

F

I

R

K

F

F

E

D

T

T

E

E

A

A

V

I

R

A

M

I

A

A

N

N

A

A

T

A

E

D

F

V

G

G

L

L

A

A

A

G

Y

Y

F

F

Y

Y

S

S

R

Q

D

D

I

P

G

A

R

Q

V

I

W

W

R

R

V

V

A

A

E

E

A

Q

L

L

E

E

Y

V

G

G

I

M

V

V

G

G

I

V

N

N

E

E

G

G

I

L

I

I

S
|
S

T

S

E

V

V

E

A

C

P

P

F
|
F

G

G

M

V

K

K

E

Q

S

S

G

G

I

L

G

G

R

R

E
|
E

G

G

S

S

K

K

Y

Y

G

G

I

I

E

D

D

E

Y

Y

L

L

E

E

I

L

K

K

Y

Y

L

V

C

C

M

Y

G

G

I

L

active site: N155 (= N168), K178 (= K191), E256 (= E266), A290 (≠ C300), E388 (= E398), E465 (= E475)
binding succinic acid: Y109 (= Y122), F156 (= F169), R163 (= R176), E256 (= E266), R284 (= R294), A290 (≠ C300), V291 (= V301), S448 (= S458), F454 (= F464)

2opxA Crystal structure of lactaldehyde dehydrogenase from escherichia coli
39% identity, 94% coverage: 25:492/497 of query aligns to 8:475/477 of 2opxA

query
sites
2opxA

F

Y

V

I

D

D

G

G

R

Q

W

F

I

V

D

T

A

W

D

R

S

G

G

D

K

A

T

W

V

I

E

D

V

V

T

V

N

N

P

P

A

A

D

T

G

E

S

A

V

V

L

I

G

S

S

R

V

I

P

P

M

D

M

G

G

Q

A

A

D

E

E

D

T

A

R

R

R

K

A

A

I

I

E

D

A

A

E

E

R

R

A

A

W

Q

P

P

A

E

W

W

R

E

A

A

L

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A

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x
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x
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x
A

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active site: N151 (= N168), K174 (= K191), E249 (= E266), C283 (= C300), E381 (= E398), A458 (≠ E475)
binding (3alpha,5beta,12alpha)-3,12-dihydroxycholan-24-oic acid: Q97 (≠ A114), L98 (≠ E115), V101 (≠ G118), F105 (≠ Y122), F152 (= F169), F155 (≠ A172), D273 (≠ A290), I277 (≠ R294), N284 (≠ V301), F312 (≠ V329), G313 (= G330), R318 (≠ T335), D320 (≠ G337), I321 (≠ A338), A322 (≠ Q339), K359 (≠ G376), Y362 (≠ F379), F440 (≠ I457), F440 (≠ I457), E441 (≠ S458)

P25553 Lactaldehyde dehydrogenase; Aldehyde dehydrogenase A; Glycolaldehyde dehydrogenase; EC 1.2.1.22; EC 1.2.1.21 from Escherichia coli (strain K12) (see 5 papers)
39% identity, 94% coverage: 25:492/497 of query aligns to 10:477/479 of P25553

query
sites
P25553

F

Y

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A

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L

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N

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R

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A

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K

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A

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x
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E

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A

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E

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A

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E

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H

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A

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L

L150 (≠ T165) binding
R161 (= R176) binding
KPSE 176:179 (≠ KPAT 191:194) binding
F180 (≠ A195) mutation to T: Can bind and use NADP(+) as coenzyme. 16-fold increase in catalytic efficiency with NAD(+) as coenzyme.
Q214 (≠ G229) binding
S230 (= S245) binding
E251 (= E266) binding
N286 (≠ V301) binding ; mutation to E: 4-fold increase in catalytic efficiency with L-lactaldehyde as substrate. Shows expanded substrate specificity.; mutation to H: 15-fold increase in catalytic efficiency with L-lactaldehyde as substrate. Shows expanded substrate specificity.; mutation to T: 6-fold increase in catalytic efficiency with L-lactaldehyde as substrate. Shows expanded substrate specificity.
R336 (≠ K351) binding
E443 (≠ S458) binding
H449 (≠ F464) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2impA Crystal structure of lactaldehyde dehydrogenase from e. Coli: the ternary complex with lactate (occupancy 0.5) and nadh. Crystals soaked with (l)-lactate. (see paper)
39% identity, 94% coverage: 25:492/497 of query aligns to 8:475/477 of 2impA

query
sites
2impA

F

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G

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T

T

A

T

A

G

I
|
I

T
x
L

P
|
P

W
|
W

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|
N

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K

P
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P

A
x
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E

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x
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A

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G

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x
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A

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G

N

N

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x
M

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x
A

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x
K

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A

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A

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E

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A

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D

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A

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T

T

I

L

L

L

A

L

D

D

V

V

T

R

P

Q

A

E

M

M

K

S

V

I

A

M

R

H

E

E

E
|
E

T

T

F

F

G

G

P

P

V

V

A

L

P

P

L

V

F

V

R

A

F

F

E

D

T

T

E

L

E

E

E

D

A

A

V

I

R

S

M

M

A

A

N

N

A

D

T

S

E

D

F

Y

G

G

L

L

A

T

A

S

Y

S

F

I

Y

Y

S

T

R

Q

D

N

I

L

G

N

R

V

V

A

W

M

R

K

V

A

A

I

E

K

A

G

L

L

E

K

Y

F

G

G

I

E

V

T

G

Y

I

I

N

N

E

R

G

E

I

N

I

F

S

E

T

A

E

M

V

Q

A

G

P

F

F

H

G

A

G

G

M

W

K

R

E

K

S

S

G

G

I

I

G

G

R

G

E
x
A

G

D

S

G

K

K

Y

H

G

G

I

L

E

H

D

E

Y

Y

L

L

E

Q

I

T

K

Q

Y

V

L

V

C

Y

M

L

active site: N151 (= N168), K174 (= K191), E249 (= E266), C283 (= C300), E381 (= E398), A458 (≠ E475)
binding 1,4-dihydronicotinamide adenine dinucleotide: I147 (= I164), L148 (≠ T165), P149 (= P166), W150 (= W167), K174 (= K191), P175 (= P192), S176 (≠ A193), E177 (≠ T194), F178 (≠ A195), G207 (≠ A224), E208 (≠ R225), G211 (= G228), Q212 (≠ G229), M225 (≠ F242), G227 (= G244), S228 (= S245), A231 (≠ I248), K234 (≠ E251), I235 (≠ L252), N328 (≠ D345), A330 (= A347), R334 (≠ K351)

2iluA Crystal structure of lactaldehyde dehydrogenase from e. Coli: the binary complex with NADPH (see paper)
39% identity, 94% coverage: 25:492/497 of query aligns to 8:475/477 of 2iluA

query
sites
2iluA

F

Y

V

I

D

D

G

G

R

Q

W

F

I

V

D

T

A

W

D

R

S

G

G

D

K

A

T

W

V

I

E

D

V

V

T

V

N

N

P

P

A

A

D

T

G

E

S

A

V

V

L

I

G

S

S

R

V

I

P

P

M

D

M

G

G

Q

A

A

D

E

E

D

T

A

R

R

R

K

A

A

I

I

E

D

A

A

E

E

R

R

A

A

W

Q

P

P

A

E

W

W

R

E

A

A

L

L

T

P

A

A

K

I

E

E

R

R

A

A

K

S

T

W

L

L

R

R

T

K

W

I

F

S

D

A

L

G

M

I

M

R

A

E

N

R

Q

A

E

S

D

E

I

I

A

S

R

A

I

L

M

I

T

V

A

E

E

E

Q

G

G

G

K

K

P

I

L

Q

A

Q

E

L

A

A

R

E

G

V

E

E

V

V

A

A

Y

F

A

T

A

A

S

D

F

Y

I

I

E

D

W

Y

F

M

A

A

E

E

E

W

G

A

K

R

R

R

V

Y

Y

E

G

G

D

E

T

I

I

I

P

Q

Q

S

H

D

L

R

P

P

G

G

R

E

R

N

I

I

V

L

T

F

K

K

E

R

P

A

I

L

G

G

V

V

T

T

A

T

A

G

I
|
I

T
x
L

P
|
P

W
|
W

N
|
N

F

F

P

P

A

F

M

L

I

I

T

A

R

R

K

K

A

M

G

A

P

P

A

A

L

L

A

L

A

T

G

G

C

N

P

T

M

I

V

V

I

I

K
|
K

P

P

A
x
S

T
x
E

A
x
F

T

T

P

P

L

N

T

N

A

A

L

I

A

A

M

F

A

A

V

K

L

I

A

V

E

D

R

E

A

I

G

G

I

L

P

P

A

R

G

G

I

V

L

F

S

N

V

L

V

V

T

L

G

G

S
x
R

A
x
G

R
x
E

A

T

I

V

G
|
G

G
x
Q

E

E

M

L

T

A

G

G

N

N

P

P

T

K

V

V

R

A

K

M

L

V

T

S

F
x
M

T

T

G
|
G

S
|
S

T

V

E

S

I
x
A

G

G

K

E

E
x
K

L
x
I

M

M

A

A

Q

T

C

A

A

A

G

K

T

N

V

I

K

T

K

K

V

V

S

C

L

L

E
|
E

L

L

G

G

N

K

A

A

P

P

F

A

L

I

V

V

F

M

N

D

D

D

A

A

D

D

L

L

D

E

E

L

A

A

V

V

K

K

G

A

A

I

I

V

A

D

S

S

K

R

Y

V

R

I

N

N

T

S

G

G

Q

Q

T

V

C
|
C

V

N

C

C

A

A

N

E

R

R

L

V

L

Y

V

V

Q

Q

S

K

G

G

V

I

Y

Y

D

D

A

Q

F

F

A

V

A

N

K

R

L

L

A

G

E

E

A

A

V

M

K

Q

A

A

L

V

K

Q

V

F

G

G

P

N

G

P

L

A

T

E

T

R

E

N

G

D

A

I

Q

A

Q

M

G

G

P

P

L

L

I

I

D
x
N

M

A

A

A

V

L

E

E

K
x
R

V

V

E

E

D

Q

H

K

I

V

R

A

D

R

A

A

T

V

E

E

K

E

G

G

A

A

R

R

V

V

V

A

L

F

G

G

K

K

R

A

H

V

E

E

L

G

G

K

G

G

S

Y

F

Y

E

P

P

P

T

T

I

L

L

L

A

L

D

D

V

V

T

R

P

Q

A

E

M

M

K

S

V

I

A

M

R

H

E

E

E
|
E

T

T

F
|
F

G

G

P

P

V

V

A

L

P

P

L

V

F

V

R

A

F

F

E

D

T

T

E

L

E

E

E

D

A

A

V

I

R

S

M

M

A

A

N

N

A

D

T

S

E

D

F

Y

G

G

L

L

A

T

A

S

Y

S

F

I

Y

Y

S

T

R

Q

D

N

I

L

G

N

R

V

V

A

W

M

R

K

V

A

A

I

E

K

A

G

L

L

E

K

Y

F

G

G

I

E

V

T

G

Y

I

I

N

N

E

R

G

E

I

N

I

F

S

E

T

A

E

M

V

Q

A

G

P

F

F

H

G

A

G

G

M

W

K

R

E

K

S

S

G

G

I

I

G

G

R

G

E
x
A

G

D

S

G

K

K

Y

H

G

G

I

L

E

H

D

E

Y

Y

L

L

E

Q

I

T

K

Q

Y

V

L

V

C

Y

M

L

active site: N151 (= N168), K174 (= K191), E249 (= E266), C283 (= C300), E381 (= E398), A458 (≠ E475)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I147 (= I164), L148 (≠ T165), P149 (= P166), W150 (= W167), K174 (= K191), S176 (≠ A193), E177 (≠ T194), F178 (≠ A195), R206 (≠ S223), G207 (≠ A224), E208 (≠ R225), G211 (= G228), Q212 (≠ G229), M225 (≠ F242), G227 (= G244), S228 (= S245), A231 (≠ I248), K234 (≠ E251), I235 (≠ L252), N328 (≠ D345), R334 (≠ K351), F383 (= F400)

5x5uA Crystal structure of alpha-ketoglutarate-semialdehyde dehydrogenase (kgsadh) complexed with NAD (see paper)
42% identity, 95% coverage: 23:492/497 of query aligns to 6:474/476 of 5x5uA

query
sites
5x5uA

Q

Q

G

L

F

L

V

I

D

D

G

G

R

E

W

W

I

V

D
|
D

A
|
A

D
x
A

S

S

G
|
G

K

K

T

T

V

I

E

D

V

V

T

V

N

N

P

P

A

A

D

T

G

G

S

K

V

P

L

I

G

G

S

R

V

V

P

A

M

H

M

A

G

G

A

I

D

A

E

D

T

L

R

D

R

R

A

A

I

L

E

A

A

A

E

Q

R

S

A

G

W

F

P

E

A

A

W

W

R

R

A

K

L

V