SitesBLAST
Comparing AZOBR_RS12025 FitnessBrowser__azobra:AZOBR_RS12025 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2cjfA Type ii dehydroquinase inhibitor complex (see paper)
57% identity, 94% coverage: 4:143/149 of query aligns to 5:145/149 of 2cjfA
- binding (1s,4s,5s)-1,4,5-trihydroxy-3-[3-(phenylthio)phenyl]cyclohex-2-ene-1-carboxylic acid: N15 (= N14), L16 (= L15), L18 (≠ M17), L19 (= L18), R22 (= R21), Y27 (= Y26), N78 (= N77), A80 (≠ G79), A81 (= A80), H84 (= H83), H105 (= H103), I106 (≠ L104), S107 (= S105), R116 (= R114)
2bt4A Type ii dehydroquinase inhibitor complex (see paper)
57% identity, 94% coverage: 4:143/149 of query aligns to 5:145/149 of 2bt4A
- binding (1s,3r,4r,5s)-1,3,4-trihydroxy-5-(3-phenoxypropyl)cyclohexanecarboxylic acid: N15 (= N14), L18 (≠ M17), R22 (= R21), Y27 (= Y26), N78 (= N77), A80 (≠ G79), A81 (= A80), H84 (= H83), H105 (= H103), I106 (≠ L104), S107 (= S105), R116 (= R114)
1gu1A Crystal structure of type ii dehydroquinase from streptomyces coelicolor complexed with 2,3-anhydro-quinic acid (see paper)
57% identity, 94% coverage: 4:143/149 of query aligns to 5:145/149 of 1gu1A
- active site: P14 (= P13), N15 (= N14), R22 (= R21), Y27 (= Y26), N78 (= N77), A81 (= A80), E103 (= E101), H105 (= H103), R112 (= R110)
- binding 2,3 -anhydro-quinic acid: Y27 (= Y26), N78 (= N77), A80 (≠ G79), A81 (= A80), H84 (= H83), H105 (= H103), I106 (≠ L104), S107 (= S105), R116 (= R114)
- binding glycerol: N15 (= N14), L16 (= L15), L19 (= L18), Y27 (= Y26)
1v1jA Crystal structure of type ii dehydroquintae dehydratase from streptomyces coelicolor in complex with 3-fluoro (see paper)
57% identity, 94% coverage: 4:143/149 of query aligns to 6:146/150 of 1v1jA
- active site: P15 (= P13), N16 (= N14), R23 (= R21), Y28 (= Y26), N79 (= N77), A82 (= A80), E104 (= E101), H106 (= H103), R113 (= R110)
- binding 2-anhydro-3-fluoro-quinic acid: Y28 (= Y26), N79 (= N77), A81 (≠ G79), A82 (= A80), H85 (= H83), H106 (= H103), I107 (≠ L104), S108 (= S105), R117 (= R114)
P15474 3-dehydroquinate dehydratase; 3-dehydroquinase; Type II DHQase; EC 4.2.1.10 from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see 2 papers)
57% identity, 94% coverage: 4:143/149 of query aligns to 7:147/157 of P15474
- R24 (= R21) mutation to A: Reduces kcat 30000-fold. Reduces KM for 3-dehydroquinate 6-fold.; mutation to K: Reduces kcat 2700-fold. Reduces KM for 3-dehydroquinate 4-fold.; mutation to Q: Reduces kcat 3100-fold. Reduces KM for 3-dehydroquinate 8-fold.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1gtzA Structure of streptomyces coelicolor type ii dehydroquinase r23a mutant in complex with dehydroshikimate (see paper)
56% identity, 94% coverage: 4:143/149 of query aligns to 5:145/149 of 1gtzA
- active site: P14 (= P13), N15 (= N14), A22 (≠ R21), Y27 (= Y26), N78 (= N77), A81 (= A80), E103 (= E101), H105 (= H103), R112 (= R110)
- binding 3-dehydroshikimate: Y27 (= Y26), A80 (≠ G79), A81 (= A80), H84 (= H83), H105 (= H103), I106 (≠ L104), S107 (= S105), R116 (= R114)
8idrC Crystal structure of apo-form of dehydroquinate dehydratase from corynebacterium glutamicum (see paper)
53% identity, 94% coverage: 6:145/149 of query aligns to 3:142/147 of 8idrC
2c4wA Type ii dehydroquinase from h. Pylori in complex with ah9095 (see paper)
49% identity, 95% coverage: 6:146/149 of query aligns to 11:154/168 of 2c4wA
- active site: P18 (= P13), N19 (= N14), R26 (= R21), Y31 (= Y26), N85 (= N77), A88 (= A80), E109 (= E101), H111 (= H103), R118 (= R110)
- binding n-tetrazol-5-yl 9-oxo-9h-xanthene-2 sulphonamide: L20 (= L15), L23 (= L18), D27 (≠ E22), G87 (= G79), H91 (= H83), H111 (= H103), L112 (= L104), T113 (≠ S105), I115 (= I107), R122 (= R114)
8iduA Crystal structure of substrate bound-form dehydroquinate dehydratase from corynebacterium glutamicum (see paper)
52% identity, 94% coverage: 6:145/149 of query aligns to 3:142/145 of 8iduA
- binding 1,3,4-trihydroxy-5-oxo-cyclohexanecarboxylic acid: Y23 (= Y26), N74 (= N77), G76 (= G79), G77 (≠ A80), H80 (= H83), H100 (= H103), I101 (≠ L104), S102 (= S105), R111 (= R114)
Q48255 3-dehydroquinate dehydratase; 3-dehydroquinase; Type II DHQase; EC 4.2.1.10 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
49% identity, 95% coverage: 6:146/149 of query aligns to 2:145/167 of Q48255
- N76 (= N77) binding
- H82 (= H83) binding
- D89 (= D90) binding
- R113 (= R114) binding
4b6rA Structure of helicobacter pylori type ii dehydroquinase inhibited by (2s)-2-(4-methoxy)benzyl-3-dehydroquinic acid (see paper)
49% identity, 95% coverage: 6:146/149 of query aligns to 2:145/157 of 4b6rA
- active site: P9 (= P13), N10 (= N14), R17 (= R21), Y22 (= Y26), N76 (= N77), A79 (= A80), E100 (= E101), H102 (= H103), R109 (= R110)
- binding (1R,2S,4S,5R)-2-(4-methoxyphenyl)methyl-1,4,5-trihydroxy-3-oxocyclohexane-1-carboxylic acid: Y22 (= Y26), N76 (= N77), G78 (= G79), A79 (= A80), H82 (= H83), D89 (= D90), L93 (= L94), H102 (= H103), L103 (= L104), T104 (≠ S105), R113 (= R114)
2xd9A Structure of helicobacter pylori type ii dehydroquinase in complex with inhibitor compound (4r,6r,7s)-4,6,7-trihydroxy-2-((e)-prop-1- enyl)-4,5,6,7-tetrahydrobenzo(b)thiophene-4-carboxylic acid (see paper)
49% identity, 95% coverage: 6:146/149 of query aligns to 2:145/153 of 2xd9A
- active site: P9 (= P13), N10 (= N14), R17 (= R21), Y22 (= Y26), N76 (= N77), A79 (= A80), E100 (= E101), H102 (= H103), R109 (= R110)
- binding (4r,6r,7s)-4,6,7-trihydroxy-2-[(1e)-prop-1-en-1-yl]-4,5,6,7-tetrahydro-1-benzothiophene-4-carboxylic acid: N10 (= N14), L14 (= L18), Y22 (= Y26), N76 (= N77), G78 (= G79), A79 (= A80), H82 (= H83), H102 (= H103), L103 (= L104), T104 (≠ S105), R113 (= R114)
2wksA Structure of helicobacter pylori type ii dehydroquinase with a new carbasugar-thiophene inhibitor. (see paper)
49% identity, 95% coverage: 6:146/149 of query aligns to 2:145/153 of 2wksA
- active site: P9 (= P13), N10 (= N14), R17 (= R21), Y22 (= Y26), N76 (= N77), A79 (= A80), E100 (= E101), H102 (= H103), R109 (= R110)
- binding (1R,4S,5R)-1,4,5-trihydroxy-3-[(5-methyl-1-benzothiophen-2-yl)methoxy]cyclohex-2-ene-1-carboxylic acid: L11 (= L15), Y22 (= Y26), N76 (= N77), G78 (= G79), A79 (= A80), H82 (= H83), H102 (= H103), L103 (= L104), T104 (≠ S105), R113 (= R114)
4b6sA Structure of helicobacter pylori type ii dehydroquinase inhibited by (2s)-2-perfluorobenzyl-3-dehydroquinic acid (see paper)
49% identity, 95% coverage: 6:146/149 of query aligns to 2:145/158 of 4b6sA
- active site: P9 (= P13), N10 (= N14), R17 (= R21), Y22 (= Y26), N76 (= N77), A79 (= A80), E100 (= E101), H102 (= H103), R109 (= R110)
- binding (1R,2S,4S,5R)-2-(2,3,4,5,6-pentafluorophenyl)methyl-1,4,5-trihydroxy-3-oxocyclohexane-1-carboxylic acid: N10 (= N14), L14 (= L18), Y22 (= Y26), N76 (= N77), G78 (= G79), A79 (= A80), H82 (= H83), H102 (= H103), L103 (= L104), T104 (≠ S105), R113 (= R114)
2xb9A Structure of helicobacter pylori type ii dehydroquinase in complex with inhibitor compound (2r)-2-(4-methoxybenzyl)-3-dehydroquinic acid (see paper)
49% identity, 95% coverage: 6:146/149 of query aligns to 2:145/158 of 2xb9A
- active site: P9 (= P13), N10 (= N14), R17 (= R21), Y22 (= Y26), N76 (= N77), A79 (= A80), E100 (= E101), H102 (= H103), R109 (= R110)
- binding (1r,2r,4s,5r)-1,4,5-trihydroxy-2-(4-methoxybenzyl)-3-oxocyclohexanecarboxylic acid: N10 (= N14), Y22 (= Y26), N76 (= N77), A79 (= A80), H82 (= H83), H102 (= H103), L103 (= L104), T104 (≠ S105), R113 (= R114)
1j2yA Crystal structure of the type ii 3-dehydroquinase (see paper)
49% identity, 95% coverage: 6:146/149 of query aligns to 2:145/158 of 1j2yA
- active site: P9 (= P13), N10 (= N14), R17 (= R21), Y22 (= Y26), N76 (= N77), A79 (= A80), E100 (= E101), H102 (= H103), R109 (= R110)
- binding 1,3,4-trihydroxy-5-oxo-cyclohexanecarboxylic acid: Y22 (= Y26), N76 (= N77), G78 (= G79), H82 (= H83), H102 (= H103), L103 (= L104), T104 (≠ S105), R113 (= R114)
2xdaA Structure of helicobacter pylori type ii dehydroquinase in complex with inhibitor compound (4r,6r,7s)-2-(2-cyclopropyl)ethyl-4,6,7- trihydroxy-4,5,6,7-tetrahydrobenzo(b)thiophene-4-carboxylic acid (see paper)
49% identity, 95% coverage: 6:146/149 of query aligns to 2:145/150 of 2xdaA
- active site: P9 (= P13), N10 (= N14), R17 (= R21), Y22 (= Y26), N76 (= N77), A79 (= A80), E100 (= E101), H102 (= H103), R109 (= R110)
- binding (4r,6r,7s)-2-(2-cyclopropylethyl)-4,6,7-trihydroxy-4,5,6,7-tetrahydro-1-benzothiophene-4-carboxylic acid: N10 (= N14), L11 (= L15), M13 (= M17), Y22 (= Y26), N76 (= N77), A79 (= A80), H82 (= H83), H102 (= H103), L103 (= L104), T104 (≠ S105), R113 (= R114)
5ydbA Crystal structure of the complex of type ii dehydroquinate dehydratase from acinetobacter baumannii with dehydroquinic acid at 1.76 angstrom resolution
51% identity, 91% coverage: 5:140/149 of query aligns to 1:137/145 of 5ydbA
- active site: P9 (= P13), N10 (= N14), R17 (= R21), Y22 (= Y26), N74 (= N77), A77 (= A80), E98 (= E101), H100 (= H103), R107 (= R110)
- binding 1,3,4-trihydroxy-5-oxo-cyclohexanecarboxylic acid: N74 (= N77), A76 (≠ G79), A77 (= A80), H80 (= H83), H100 (= H103), L101 (= L104), S102 (= S105), R111 (= R114)
5b6pB Structure of the dodecameric type-ii dehydrogenate dehydratase from acinetobacter baumannii at 2.00 a resolution (see paper)
51% identity, 91% coverage: 5:140/149 of query aligns to 1:137/145 of 5b6pB
- active site: P9 (= P13), N10 (= N14), R17 (= R21), Y22 (= Y26), N74 (= N77), A77 (= A80), E98 (= E101), H100 (= H103), R107 (= R110)
- binding sulfate ion: N74 (= N77), H100 (= H103), L101 (= L104), S102 (= S105)
4cl0A Structure of the mycobacterium tuberculosis type ii dehydroquinase inhibited by a 3-dehydroquinic acid derivative
47% identity, 91% coverage: 9:143/149 of query aligns to 5:139/140 of 4cl0A
- active site: P9 (= P13), N10 (= N14), R17 (= R21), Y22 (= Y26), N73 (= N77), G76 (≠ A80), E97 (= E101), H99 (= H103), R106 (= R110)
- binding (2r)-2-methyl-3-dehydroquinic acid: R17 (= R21), Y22 (= Y26), N73 (= N77), G75 (= G79), G76 (≠ A80), H79 (= H83), H99 (= H103), I100 (≠ L104), S101 (= S105), R110 (= R114)
Query Sequence
>AZOBR_RS12025 FitnessBrowser__azobra:AZOBR_RS12025
VAIDASVLVLNGPNLNMLGVREPHIYGSMTLDDLEGACQERAEQLGLAIDFRQSNHEGEL
VSWIQQARTEHDGIVINAGAYSHTSVAIMDALILSELPIVEVHLSNIYKREAIRHHSHIS
AVAKGMICGFGPHGYLLALDAMANILERD
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory