SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AZOBR_RS14105 FitnessBrowser__azobra:AZOBR_RS14105 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

P48636 Cytokinin riboside 5'-monophosphate phosphoribohydrolase; AMP nucleosidase; PaLOG; EC 3.2.2.n1; EC 3.2.2.4 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
60% identity, 97% coverage: 3:188/192 of query aligns to 6:191/195 of P48636

query
sites
P48636

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E74 (= E71) binding
M89 (= M86) mutation to A: Almost complete loss of activity.
R92 (= R89) mutation to A: Almost complete loss of activity.
RK 92:93 (= RK 89:90) binding
109:115 (vs. 106:112, 86% identical) binding
T112 (= T109) mutation to A: Almost complete loss of activity.
E114 (= E111) mutation to A: Almost complete loss of activity.
E115 (= E112) mutation to A: Almost complete loss of activity.
E118 (= E115) mutation to A: Retains 8% of wild-type activity.

5zbkA Crystal structure of type-i log from pseudomonas aeruginosa pao1 in complex with amp (see paper)
61% identity, 93% coverage: 3:180/192 of query aligns to 5:182/182 of 5zbkA

query
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5zbkA

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binding adenosine monophosphate: A11 (≠ S9), E73 (= E71), M88 (= M86), R91 (= R89), K92 (= K90), G108 (= G106), G110 (= G108), T111 (= T109)

Q5ZC82 Cytokinin riboside 5'-monophosphate phosphoribohydrolase LOG; Protein LONELY GUY; EC 3.2.2.n1 from Oryza sativa subsp. japonica (Rice) (see paper)
51% identity, 93% coverage: 2:180/192 of query aligns to 37:215/242 of Q5ZC82

query
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Q5ZC82

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G73 (= G38) mutation to D: In LOG-4; decreased activity.

5zblD Crystal structure of type-i log from corynebacterium glutamicum in complex with amp (see paper)
47% identity, 93% coverage: 2:180/192 of query aligns to 4:181/181 of 5zblD

query
sites
5zblD

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binding adenosine monophosphate: S11 (= S9), M47 (= M45), R90 (= R89), K91 (= K90), G107 (= G106), G109 (= G108), T110 (= T109), E112 (= E111), E113 (= E112)

5ajuA Crystal structure of ligand-free phosphoribohydroxylase lonely guy from claviceps purpurea in complex with phosphoribose (see paper)
40% identity, 93% coverage: 2:179/192 of query aligns to 4:204/217 of 5ajuA

query
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5ajuA

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binding 5-O-phosphono-beta-D-ribofuranose: S11 (= S9), R108 (= R89), G131 (= G106), Y132 (≠ I107), G133 (= G108), T134 (= T109), E137 (= E112)

O05306 Cytokinin riboside 5'-monophosphate phosphoribohydrolase; Protein LONELY GUY homolog; LOG homolog; EC 3.2.2.n1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
39% identity, 91% coverage: 3:176/192 of query aligns to 13:185/187 of O05306

query
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O05306

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M54 (= M45) mutation to A: Large decrease in catalytic activity.
K74 (≠ Q65) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup); mutation to A: Abrogates pupylation. This mutant accumulates in M.tuberculosis with a functional proteasome system and sensitizes M.tuberculosis to NO. Decrease in catalytic activity.
E80 (= E71) mutation to A: Large decrease in catalytic activity.
R98 (= R89) mutation to A: Loss of catalytic activity.
T118 (= T109) mutation to A: Loss of catalytic activity.
DE 120:121 (≠ EE 111:112) mutation to AA: Loss of catalytic activity.

7w2iA Crystal structure of log (rv1205) from mycobacterium tuberculosis (see paper)
39% identity, 91% coverage: 3:176/192 of query aligns to 9:181/183 of 7w2iA

query
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7w2iA

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binding magnesium ion: D120 (≠ E115), D124 (≠ W119)

3sbxA Crystal structure of a putative uncharacterized protein from mycobacterium marinum bound to adenosine 5'-monophosphate amp (see paper)
39% identity, 91% coverage: 3:176/192 of query aligns to 4:176/177 of 3sbxA

query
sites
3sbxA

I

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binding adenosine monophosphate: E71 (= E71), M86 (= M86), R89 (= R89), K90 (= K90), G106 (= G106), G108 (= G108), T109 (= T109), D111 (≠ E111), E112 (= E112)

Query Sequence

>AZOBR_RS14105 FitnessBrowser__azobra:AZOBR_RS14105
MRICVYAGSNPGTNPAYGEAAEQLGRHMAERGIGLVYGGGRTGLMGRIADSVLAAGGTVT
GIIPQFLMDKEVGHQGLQELRIVATMHERKALMAELSDGFIALPGGIGTLEELFEVWTWA
QLGRHDKPCGLLNASGFYDGLAGFLDHVAGERFMQPKHRDMLIVRDTAPGILDAFAAYEP
PAVRKWLDEART

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory