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Comparing AZOBR_RS14735 FitnessBrowser__azobra:AZOBR_RS14735 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q04797 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Bacillus subtilis (strain 168) (see paper)
47% identity, 96% coverage: 10:334/337 of query aligns to 6:338/346 of Q04797
- S98 (= S102) modified: Phosphoserine
- Y146 (≠ F149) modified: Phosphotyrosine
P23247 Aspartate-semialdehyde dehydrogenase 2; ASA dehydrogenase 2; ASADH 2; Aspartate-beta-semialdehyde dehydrogenase 2; EC 1.2.1.11 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see paper)
45% identity, 96% coverage: 11:335/337 of query aligns to 7:331/337 of P23247
- C132 (= C133) active site, Acyl-thioester intermediate
2r00C Crystal structure of aspartate semialdehyde dehydrogenase ii complexed with asa from vibrio cholerae (see paper)
45% identity, 96% coverage: 11:335/337 of query aligns to 6:330/336 of 2r00C
4r5hA Crystal structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide-adenine-dinucleotide-phosphate and 3-carboxy-propenyl- phthalic acid (see paper)
43% identity, 96% coverage: 11:334/337 of query aligns to 4:338/359 of 4r5hA
- binding 3-[(1E)-3-carboxyprop-1-en-1-yl]benzene-1,2-dicarboxylic acid: S73 (≠ G80), T94 (≠ S101), S95 (= S102), R98 (= R105), N126 (= N132), C127 (= C133), Q154 (= Q160), G158 (= G164), K222 (= K217), R244 (= R239), H251 (= H246)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G15), T10 (= T17), G11 (= G18), A12 (= A19), V13 (= V20), A35 (= A42), S36 (= S43), R38 (= R45), S39 (= S46), T56 (≠ F63), S70 (≠ A77), A71 (≠ V78), G72 (≠ S79), T75 (≠ F82), N93 (= N100), T94 (≠ S101), P125 (= P131), N126 (= N132), C127 (= C133), G160 (= G166), M161 (≠ A167), G328 (= G324)
4r4jA Crystal structure of complex sp_asadh with 3-carboxypropyl-phthalic acid and nicotinamide adenine dinucleotide phosphate (see paper)
43% identity, 96% coverage: 11:334/337 of query aligns to 4:338/359 of 4r4jA
- binding 3-(3-carboxypropyl)benzene-1,2-dicarboxylic acid: T94 (≠ S101), S95 (= S102), R98 (= R105), N126 (= N132), C127 (= C133), Q154 (= Q160), G158 (= G164), E219 (= E214), K222 (= K217), R244 (= R239), H251 (= H246)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G15), T10 (= T17), G11 (= G18), A12 (= A19), V13 (= V20), A35 (= A42), S36 (= S43), R38 (= R45), S39 (= S46), T56 (≠ F63), S70 (≠ A77), A71 (≠ V78), G72 (≠ S79), T75 (≠ F82), N93 (= N100), T94 (≠ S101), N126 (= N132), C127 (= C133), G160 (= G166), M161 (≠ A167), G328 (= G324)
3q11A Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with NADP and aspartyl beta- difluorophosphonate (see paper)
43% identity, 96% coverage: 11:334/337 of query aligns to 4:338/358 of 3q11A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G15), T10 (= T17), G11 (= G18), A35 (= A42), S36 (= S43), R38 (= R45), S39 (= S46), T56 (≠ F63), A71 (≠ V78), T75 (≠ F82), G160 (= G166), M161 (≠ A167), G162 (≠ E168)
- binding 5,5-difluoro-4-oxo-5-phosphono-D-norvaline: R98 (= R105), N126 (= N132), C127 (= C133), Q154 (= Q160), G158 (= G164), E219 (= E214), K222 (= K217), R244 (= R239)
3pyxB Crystals structure of aspartate beta-semialdehyde dehydrogenase complex with NADP and 2-aminoterephthalate (see paper)
43% identity, 96% coverage: 11:334/337 of query aligns to 4:338/359 of 3pyxB
- binding 2-aminobenzene-1,4-dicarboxylic acid: R98 (= R105), G158 (= G164), E219 (= E214), K222 (= K217), R244 (= R239)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G15), T10 (= T17), G11 (= G18), A12 (= A19), V13 (= V20), A35 (= A42), S36 (= S43), R38 (= R45), S39 (= S46), T56 (≠ F63), S70 (≠ A77), A71 (≠ V78), G72 (≠ S79), T75 (≠ F82), C127 (= C133), S157 (= S163), G158 (= G164), G160 (= G166), M161 (≠ A167), N324 (≠ Q320), L325 (= L321)
4r54A Complex crystal structure of sp-aspartate-semialdehyde-dehydrogenase with 3-carboxy-ethyl-phthalic acid (see paper)
43% identity, 96% coverage: 11:334/337 of query aligns to 4:338/357 of 4r54A
- binding 3-(2-carboxyethyl)benzene-1,2-dicarboxylic acid: G72 (≠ S79), S73 (≠ G80), T94 (≠ S101), S95 (= S102), R98 (= R105), K222 (= K217)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G15), T10 (= T17), G11 (= G18), A12 (= A19), V13 (= V20), A35 (= A42), S36 (= S43), R38 (= R45), S39 (= S46), T56 (≠ F63), S70 (≠ A77), A71 (≠ V78), G72 (≠ S79), T75 (≠ F82), N93 (= N100), T94 (≠ S101), N126 (= N132), C127 (= C133), G160 (= G166), G328 (= G324)
4r41A Complex crystal structure of 4-nitro-2-phosphono-benzoic acid with sp- aspartate-semialdehyde dehydrogenase and nicotinamide-dinucleotide (see paper)
43% identity, 96% coverage: 11:334/337 of query aligns to 4:338/357 of 4r41A
- binding 4-nitro-2-phosphonobenzoic acid: S70 (≠ A77), G72 (≠ S79), S73 (≠ G80), N93 (= N100), T94 (≠ S101), S95 (= S102), R98 (= R105), K222 (= K217)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G15), T10 (= T17), G11 (= G18), A12 (= A19), A35 (= A42), S36 (= S43), R38 (= R45), S39 (= S46), T56 (≠ F63), A71 (≠ V78), G160 (= G166), M161 (≠ A167), G162 (≠ E168)
4r3nA Crystal structure of the ternary complex of sp-asadh with NADP and 1, 2,3-benzenetricarboxylic acid (see paper)
43% identity, 96% coverage: 11:334/337 of query aligns to 4:338/357 of 4r3nA
- active site: C127 (= C133), Q154 (= Q160), R244 (= R239), H251 (= H246)
- binding benzene-1,2,3-tricarboxylic acid: S73 (≠ G80), T94 (≠ S101), S95 (= S102), R98 (= R105), N126 (= N132), K222 (= K217)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G15), T10 (= T17), G11 (= G18), A12 (= A19), V13 (= V20), A35 (= A42), S36 (= S43), S39 (= S46), T56 (≠ F63), S70 (≠ A77), A71 (≠ V78), G72 (≠ S79), N93 (= N100), T94 (≠ S101), N126 (= N132), C127 (= C133), G160 (= G166), M161 (≠ A167), G328 (= G324)
3q1lA Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with cysteamine bound covalently to cys 128 (see paper)
43% identity, 96% coverage: 11:334/337 of query aligns to 4:338/357 of 3q1lA
3pwsA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
43% identity, 96% coverage: 11:334/337 of query aligns to 4:338/357 of 3pwsA
- binding (2R)-2-aminohexanedioic acid: R98 (= R105), N126 (= N132), G158 (= G164), I208 (= I203), E219 (= E214), K222 (= K217), R244 (= R239)
- binding adenosine-2'-5'-diphosphate: G8 (= G15), T10 (= T17), G11 (= G18), A12 (= A19), A35 (= A42), S36 (= S43), R38 (= R45), S39 (= S46), T56 (≠ F63), A71 (≠ V78), T75 (≠ F82), G160 (= G166), M161 (≠ A167)
3pwkA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
43% identity, 96% coverage: 11:334/337 of query aligns to 4:338/357 of 3pwkA
- binding 5'-o-monophosphoryladenylyl(2'->5')adenylyl(2'->5')adenosine: G8 (= G15), T10 (= T17), G11 (= G18), A12 (= A19), A35 (= A42), S36 (= S43), R38 (= R45), S39 (= S46), T56 (≠ F63), A71 (≠ V78), T75 (≠ F82), G160 (= G166)
- binding trans-cyclohexane-1,4-dicarboxylic acid: R98 (= R105), N126 (= N132), G158 (= G164), A159 (= A165), E219 (= E214), K222 (= K217), R244 (= R239)
2gz3A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP and aspartate- semialdehyde (see paper)
43% identity, 96% coverage: 11:334/337 of query aligns to 4:338/357 of 2gz3A
- active site: C127 (= C133), Q154 (= Q160), R244 (= R239), H251 (= H246)
- binding (2r)-2-amino-4-oxobutanoic acid: C127 (= C133), Q154 (= Q160), G158 (= G164), E219 (= E214), R244 (= R239), H251 (= H246)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G15), T10 (= T17), G11 (= G18), A12 (= A19), V13 (= V20), A35 (= A42), S36 (= S43), R38 (= R45), S39 (= S46), T56 (≠ F63), S70 (≠ A77), A71 (≠ V78), G72 (≠ S79), T75 (≠ F82), N93 (= N100), G158 (= G164), G160 (= G166), M161 (≠ A167), N324 (≠ Q320), A329 (= A325)
2gz2A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with 2',5'-adp (see paper)
43% identity, 96% coverage: 11:334/337 of query aligns to 4:338/357 of 2gz2A
- active site: C127 (= C133), Q154 (= Q160), R244 (= R239), H251 (= H246)
- binding adenosine-2'-5'-diphosphate: G8 (= G15), T10 (= T17), G11 (= G18), A35 (= A42), S36 (= S43), R38 (= R45), S39 (= S46), T56 (≠ F63), A71 (≠ V78), T75 (≠ F82)
2gz1A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP (see paper)
43% identity, 96% coverage: 11:334/337 of query aligns to 4:338/357 of 2gz1A
- active site: C127 (= C133), Q154 (= Q160), R244 (= R239), H251 (= H246)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G15), T10 (= T17), G11 (= G18), A12 (= A19), V13 (= V20), A35 (= A42), S36 (= S43), R38 (= R45), S39 (= S46), T56 (≠ F63), S70 (≠ A77), A71 (≠ V78), G72 (≠ S79), T75 (≠ F82), N93 (= N100), S157 (= S163), G158 (= G164), G160 (= G166), M161 (≠ A167), N324 (≠ Q320), L325 (= L321)
4r51A Crystal complex structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide adenine dinucleotide phosphate and phthalic acid (see paper)
43% identity, 96% coverage: 11:334/337 of query aligns to 4:338/360 of 4r51A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G15), T10 (= T17), G11 (= G18), A12 (= A19), V13 (= V20), A35 (= A42), S36 (= S43), S39 (= S46), T56 (≠ F63), S70 (≠ A77), A71 (≠ V78), G72 (≠ S79), N93 (= N100), T94 (≠ S101), N126 (= N132), C127 (= C133), G160 (= G166), M161 (≠ A167), G328 (= G324)
- binding phthalic acid: S73 (≠ G80), T94 (≠ S101), S95 (= S102), R98 (= R105), N126 (= N132), K222 (= K217)
3pylC Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with d-2,3-diaminopropionate (see paper)
43% identity, 96% coverage: 11:334/337 of query aligns to 4:338/361 of 3pylC
3tz6A Crystal structure of aspartate semialdehyde dehydrogenase complexed with inhibitor smcs (cys) and phosphate from mycobacterium tuberculosis h37rv (see paper)
43% identity, 96% coverage: 11:335/337 of query aligns to 4:339/342 of 3tz6A
- active site: C129 (= C133), Q156 (= Q160), R248 (= R239), H255 (= H246)
- binding cysteine: C129 (= C133), Q156 (= Q160), G160 (= G164), E223 (= E214), R248 (= R239), H255 (= H246)
- binding glycerol: S108 (≠ P115), G187 (vs. gap), F192 (vs. gap), P201 (= P194), Q225 (≠ M216), R228 (≠ T219), F229 (≠ W220), Q335 (= Q331), E338 (= E334), L339 (= L335)
- binding sulfate ion: R98 (= R105), H117 (= H120), R119 (≠ M122), N128 (= N132), C129 (= C133), K226 (= K217), E270 (≠ D261), R273 (= R264)
P44801 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) (see 2 papers)
30% identity, 94% coverage: 9:326/337 of query aligns to 2:359/371 of P44801
- RGMV 10:13 (≠ TGAV 17:20) binding
- TS 37:38 (≠ ER 44:45) binding
- Q74 (≠ V78) binding
- R103 (= R105) binding ; mutation to K: 2-fold increase in affinity for ASA, 23-fold decrease in affinity for phosphate, and 275-fold decrease in activity.; mutation to L: 7-fold increase in affinity for ASA, 150-fold decrease in affinity for phosphate, and 1400-fold decrease in activity.
- C136 (= C133) active site, Acyl-thioester intermediate
- S166 (= S163) binding
- E243 (= E214) mutation to D: No change in affinity for ASA and 82-fold decrease in activity.
- K246 (= K217) binding ; mutation to R: 2-fold increase in affinity for ASA, nearly no change in affinity for phosphate, and 30-fold decrease in activity.
- R270 (= R239) mutation to K: 2-fold decrease in affinity for ASA and 825-fold decrease in activity.
- H277 (= H246) active site, Proton acceptor
- Q353 (= Q320) binding
Query Sequence
>AZOBR_RS14735 FitnessBrowser__azobra:AZOBR_RS14735
MSTSSPSPRRVAIVGATGAVGREMVDVLHRRSFPVAELGLFASERSAGRTVETPFGEKTL
VAFDVEKVKGYDIVLLAVSGDFAKEHAPAIAAGGALVIDNSSAFRYDDNVPLIVPEINAH
AMGDAKLIANPNCTTAIAVVALGPLHKAFGLKRVIVSTYQATSGAGAEGMAELEEQTRNQ
LDGKPVVNSVFRHPIPFNLIPQIDAFQENGYTKEEMKVTWETRKILEIPDLPVSCTAVRI
PTYRAHSEAITVETLSPVTPDAARAVLADAPGVVVKDVPADGVYPMPLNATGQFDVEVGR
IRTSLIFGDHGLDLFVCGDQLLKGAALNAVQIAELSL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory