SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AZOBR_RS16135 AZOBR_RS16135 sorbitol dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4ilkA Crystal structure of short chain alcohol dehydrogenase (rspb) from e. Coli cft073 (efi target efi-506413) complexed with cofactor nadh
31% identity, 99% coverage: 1:332/334 of query aligns to 8:335/337 of 4ilkA

query
sites
4ilkA

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active site: C40 (= C33), G41 (= G34), S42 (= S35), H45 (≠ S38), H59 (= H55), E60 (= E56), C89 (= C86), C92 (= C89), C95 (= C92), C103 (= C100), A107 (≠ R104), P145 (= P141), A149 (≠ G145), K332 (= K329)
binding manganese (ii) ion: C40 (= C33), H59 (= H55), E60 (= E56), E144 (= E140)
binding 1,4-dihydronicotinamide adenine dinucleotide: I148 (≠ V144), Y167 (≠ L163), G168 (= G164), G170 (= G166), P171 (≠ M167), I172 (= I168), A192 (≠ V186), D193 (= D187), R194 (≠ I188), I195 (≠ G189), R198 (≠ K192), N213 (≠ A207), A235 (= A231), A236 (≠ V232), C237 (≠ G233), I241 (≠ T237), M258 (≠ I254), F260 (≠ Y256), S282 (= S278), R283 (= R279), L284 (≠ A281)
binding zinc ion: C89 (= C86), C92 (= C89), C95 (= C92), C103 (= C100)

O58389 L-threonine 3-dehydrogenase; L-ThrDH; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 2 papers)
31% identity, 92% coverage: 12:317/334 of query aligns to 21:332/348 of O58389

query
sites
O58389

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C42 (= C33) binding
T44 (≠ S35) mutation to A: Total loss of enzymatic activity.
H67 (= H55) binding
E68 (= E56) binding
C97 (= C86) binding
C100 (= C89) binding
C103 (= C92) binding
C111 (= C100) binding
E152 (= E140) mutation E->A,Q: Almost complete loss of enzymatic activity.; mutation to C: 120-fold decrease in catalytic efficiency.; mutation to D: Shows 3-fold higher turnover rate and reduced affinities toward L-threonine and NAD(+), compared to wild-type.; mutation to K: Total loss of enzymatic activity.
L179 (≠ I168) binding
E199 (≠ D187) binding ; mutation to A: Large decrease in affinity for NAD(+).
R204 (≠ K192) binding ; mutation to A: Large decrease in affinity for NAD(+).
LGL 266:268 (≠ IGY 254:256) binding
IT 291:292 (≠ -S 278) binding
R294 (≠ N280) mutation to A: 4000-fold decrease in catalytic efficiency.

2dfvA Hyperthermophilic threonine dehydrogenase from pyrococcus horikoshii (see paper)
31% identity, 92% coverage: 12:317/334 of query aligns to 19:330/346 of 2dfvA

query
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2dfvA

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active site: C40 (= C33), G41 (= G34), T42 (≠ S35), H45 (≠ S38), H65 (= H55), E66 (= E56), C95 (= C86), C98 (= C89), C101 (= C92), C109 (= C100), K113 (≠ R104), P151 (= P141), A155 (≠ G145)
binding nicotinamide-adenine-dinucleotide: N154 (≠ V144), G173 (= G164), G175 (= G166), P176 (≠ M167), L177 (≠ I168), S196 (≠ V186), E197 (≠ D187), P198 (≠ I188), R202 (≠ K192), P217 (≠ A207), F241 (≠ A231), S242 (≠ V232), G243 (= G233), A244 (≠ L234), A247 (≠ T237), L264 (≠ I254), G265 (= G255), L266 (≠ Y256), I289 (vs. gap), T290 (≠ S278)
binding zinc ion: C95 (= C86), C98 (= C89), C101 (= C92), C109 (= C100)

Sites not aligning to the query:

active site: 340

4ejmA Crystal structure of a putative zinc-binding dehydrogenase (target psi-012003) from sinorhizobium meliloti 1021 bound to NADP
32% identity, 90% coverage: 2:301/334 of query aligns to 9:325/342 of 4ejmA

query
sites
4ejmA

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N

P

D

L

T

T

L

L

A

D

P

P

R

V

H

H

L

G

A

A

L

F

V

C

E

E

P
|
P

L

L

S

A

V
x
C

G
x
C

F

L

H

H

A

G

A

V

A

D

R

L

G

S

R

G

V

I

E

K

A

A

A

G

D

S

T

T

V

V

L

A

V

I

L

L

G
|
G

C
x
G

G
|
G

M
x
V

I
|
I

G

G

M

L

G

L

A

T

I

V

A

Q

G

L

S

A

V

R

H

L

R

A

G

G

A

A

T

T

V

T

I

V

A

I

V

L

D
x
S

I
x
T

G
x
R

E
x
Q

A

A

-

T

K
|
K

T

R

G

R

L

L

A

A

R

E

Y

V

G

G

A

A

A

T

H

A

T

T

I

V

D

D

A
x
P

G

S

K

A

E

G

D

D

V

V

A

V

A

E

R

A

I

I

A

A

-

G

-

P

-

V

E

G

L

L

T

V

N

P

G

G

D

-

G

G

A

V

D

D

V

V

I

I

E

E

A
x
C

V
x
A

G
|
G

L
x
V

P

A

A
x
E

T
|
T

F

V

T

K

Q

Q

A

S

I

T

D

R

L

L

V

A

S

K

F

A

A

G

G

G

R

T

V

V

Y

I

I
x
L

G
|
G

Y
x
V

A

L

K

P

E

Q

P

G

V

E

S

K

Y

V

K

E

T

I

Q

E

F

P

F

F

N

D

L

I

-

L

-

F

K

R

E

E

L

L

D

R

I

V

M

L

G

G

S
|
S

-
x
F

-
x
I

-

N

-

P

-

F

-

V

-

H

R

R

N

R

A

A

T

A

-

D

-

L

-

V

-

A

-

T

-

G

S

A

E

I

D

E

F

I

Q

D

A

R

V

M

I

I

H

S

Y

R

L

R

E

I

T

S

L

L

D

D

H

E

A

A

P

P

D

D

active site: C40 (= C33), G41 (= G34), T42 (≠ S35), H45 (≠ S38), H61 (= H55), E62 (= E56), C91 (= C86), C94 (= C89), C97 (= C92), C105 (= C100), R109 (= R104), P147 (= P141), C151 (≠ G145)
binding nadp nicotinamide-adenine-dinucleotide phosphate: C150 (≠ V144), G170 (= G164), G171 (≠ C165), G172 (= G166), V173 (≠ M167), I174 (= I168), S193 (≠ D187), T194 (≠ I188), R195 (≠ G189), Q196 (≠ E190), K199 (= K192), P214 (≠ A207), C240 (≠ A231), A241 (≠ V232), G242 (= G233), V243 (≠ L234), E245 (≠ A236), T246 (= T237), L263 (≠ I254), G264 (= G255), V265 (≠ Y256), S289 (= S278), F290 (vs. gap), I291 (vs. gap)
binding zinc ion: C91 (= C86), C94 (= C89), C97 (= C92), C105 (= C100)

Sites not aligning to the query:

active site: 337

4ej6A Crystal structure of a putative zinc-binding dehydrogenase (target psi-012003) from sinorhizobium meliloti 1021
32% identity, 90% coverage: 2:301/334 of query aligns to 9:325/343 of 4ej6A

query
sites
4ej6A

I

L

E

E

G

S

V

V

D

G

V

N

C

I

A

S

I

V

H

R

E

N

V

V

A

G

E

I

P

P

T

E

P

P

G

G

P

P

G

D

E

D

V

L

L

L

L

V

A

K

I

V

R

E

H

A

V

C

A

G

L

I

C
|
C

G
|
G

S
x
T

D

D

L

R

S
x
H

T

L

F

L

K

H

G

G

L

E

N

F

P

P

L

-

V

S

S

T

L

P

P

P

R

V

I

T

P

L

G

G

H
|
H

E
|
E

I

F

G

C

G

G

E

I

I

V

A

V

A

E

V

A

G

G

E

S

G

A

V

V

P

-

A

R

E

D

Y

I

T

A

V

P

G

G

R

A

R

R

A

I

I

T

V

G

I

D

P

P

Y

N

T

I

A

S

C
|
C

G

G

T

R

C
|
C

P

P

S

Q

C
|
C

R

Q

K

A

G

G

R

R

V

V

N

N

A

L

C
|
C

R

R

S

N

N

L

R
|
R

T

A

L

I

G

G

V

I

Q

H

Q

R

N

D

G

G

L

F

A

A

E

E

R

Y

I

V

V

L

L

V

P

P

Y

R

G

K

K

Q

L

A

I

F

-

E

L

I

N

P

D

L

T

T

L

L

A

D

P

P

R

V

H

H

L

G

A

A

L

F

V

C

E

E

P
|
P

L

L

S

A

V

C

G
x
C

F

L

H

H

A

G

A

V

A

D

R

L

G

S

R

G

V

I

E

K

A

A

A

G

D

S

T

T

V

V

L

A

V

I

L

L

G

G

C

G

G

G

M

V

I

I

G

G

M

L

G

L

A

T

I

V

A

Q

G

L

S

A

V

R

H

L

R

A

G

G

A

A

T

T

V

T

I

V

A

I

V

L

D

S

I

T

G

R

E

Q

A

A

-

T

K

K

T

R

G

R

L

L

A

A

R

E

Y

V

G

G

A

A

A

T

H

A

T

T

I

V

D

D

A

P

G

S

K

A

E

G

D

D

V

V

A

V

A

E

R

A

I

I

A

A

-

G

-

P

-

V

E

G

L

L

T

V

N

P

G

G

D

-

G

G

A

V

D

D

V

V

I

I

E

E

A

C

V

A

G

G

L

V

P

A

A

E

T

T

F

V

T

K

Q

Q

A

S

I

T

D

R

L

L

V

A

S

K

F

A

A

G

G

G

R

T

V

V

Y

I

I

L

G

G

Y

V

A

L

K

P

E

Q

P

G

V

E

S

K

Y

V

K

E

T

I

Q

E

F

P

F

F

N

D

L

I

-

L

-

F

K

R

E

E

L

L

D

R

I

V

M

L

G

G

S

S

-

F

-

I

-

N

-

P

-

F

-

V

-

H

R

R

N

R

A

A

T

A

-

D

-

L

-

V

-

A

-

T

-

G

S

A

E

I

D

E

F

I

Q

D

A

R

V

M

I

I

H

S

Y

R

L

R

E

I

T

S

L

L

D

D

H

E

A

A

P

P

D

D

active site: C40 (= C33), G41 (= G34), T42 (≠ S35), H45 (≠ S38), H61 (= H55), E62 (= E56), C91 (= C86), C94 (= C89), C97 (= C92), C105 (= C100), R109 (= R104), P147 (= P141), C151 (≠ G145)
binding zinc ion: C91 (= C86), C94 (= C89), C97 (= C92), C105 (= C100)

Sites not aligning to the query:

active site: 337

5k1sA Crystal structure of aibc (see paper)
33% identity, 90% coverage: 16:316/334 of query aligns to 37:339/358 of 5k1sA

query
sites
5k1sA

P

P

T

R

P

P

G

G

P

R

G

G

E

E

V

V

L

L

A

R

I

V

R

H

H

A

V

C

A

G

L

V

C
|
C

G
x
Y

S
x
H

D

D

L

V

S
x
I

T

N

F

R

K

R

G

G

L

N

N

L

P

P

L

R

V

T

S

S

L

V

P

P

R

A

I

I

P

L

G

G

H
|
H

E
|
E

I

A

G

A

G

G

E

E

I

V

A

I

A

E

V

V

G

G

E

P

G

D

V

T

P

P

A

G

E

-

Y

W

T

K

V

T

G

G

R

D

R

R

A

A

I

A

V

T

I

L

P

Q

Y

R

T

M

A

S

C
|
C

G

G

T

D

C
|
C

P

A

S

L

C
|
C

R

R

K

S

G

G

R

R

V

N

N

S

A

L

C
|
C

R

K

S

T

-

D

N
|
N

R

R

T

F

L

F

G

G

V

E

Q

E

Q

L

N

P

G

G

L

Y

A

A

E

Q

R

F

I

M

V

V

L

A

P

P

Y

V

G

G

K

G

L

L

I

G

L

R

N

V

D

P

T

A

L

S

A

L

P

P

R

W

H

N

L

E

A

A

L

A

V

T

E

V

P

C

L
x
C

S

T

V

T

G

G

-
x
T

-

A

F

V

H

H

A

T

A

V

-

R

A

T

R

R

G

G

R

K

V

V

E

R

A

A

A

G

D

E

T

T

V

V

L

L

V

I

L

T

G

G

C

A

-

S

G

G

M

G

I

V

G

G

M

L

G

S

A

S

I

V

A

Q

G

L

S

A

V

R

H

L

R

D

G

G

A

A

T

R

V

V

I

I

A

A

V

V

D

T

I

S

G

S

E

E

A

A

K

K

T

V

G

Q

L

A

A

L

R

K

R

E

Y

A

G

G

A

A

A

D

H

E

T

V

I

I

D

V

A

S

G

R

K

G

E

L

D

D

V

F

A

A

A

S

R

D

I

V

A

R

E

K

L

R

T

T

N

Q

G

G

D

A

G

G

A

V

D

D

V

V

V

A

I

V

E

E

A

I

V

V

G

G

L

-

P

S

A

A

T

T

F

F

T

D

Q

Q

A

T

I

L

D

K

L

S

V

M

S

A

F

P

A

G

G

G

R

R

V

V

Y

V

I

V

G

G

Y

N

A

L

K

E

E

S

P

G

-

M

V

V

S

Q

Y

L

K

N

T

P

Q

G

F

L

F

V

N

I

L

V

K

K

E

E

L

L

D

E

I

I

M

L

G

G

S

A

R

Y

N

A

A

T

T

T

S

Q

E

A

D

E

F

L

Q

D

A

E

V

A

I

L

H

R

Y

L

L

T

E

A

T

T

L

-

D

-

H

G

A

G

P

V

D

R

D

Q

L

F

I

V

S

T

K

D

V

A

F

V

P

P

F

L

E

A

E

E

A

A

D

A

K

K

A

A

active site: C54 (= C33), Y55 (≠ G34), H56 (≠ S35), I59 (≠ S38), E77 (= E56), C106 (= C86), C109 (= C89), C112 (= C92), C120 (= C100), N124 (= N103), C163 (≠ L142), T167 (vs. gap)
binding zinc ion: C54 (= C33), H76 (= H55), C109 (= C89), C112 (= C92), C120 (= C100), C163 (≠ L142)

Sites not aligning to the query:

active site: 351

5ylnA Zinc dependent alcohol dehydrogenase 2 from streptococcus pneumonia - apo form
30% identity, 97% coverage: 12:334/334 of query aligns to 20:343/348 of 5ylnA

query
sites
5ylnA

E

D

V

V

A

D

E

K

P

P

T

V

P

I

G

R

P

K

G

P

E

T

V

A

L

I

L

V

A

R

I

I

R

V

H

K

V

T

A

T

L

I

C
|
C

G

G

S
x
T

D

D

L

L

S
x
H

T

I

F

I

K

K

G

G

L

D

N

V

P

P

L

T

V

C

S

Q

L

S

P

G

R

T

I

I

P

L

G

G

H
|
H

E

E

I

G

G

I

G

G

E

I

I

V

A

E

V

V

G

G

E

E

G

G

V

V

P

-

A

S

E

N

Y

F

T

K

V

K

G

G

R

D

R

K

A

V

I

L

V

I

I

S

P

C

Y

V

T

C

A

A

C
|
C

G

G

T

K

C
|
C

P

Y

S

Y

C
|
C

R

K

K

K

G

G

R

I

V

Y

N

A

A

H

C
|
C

R

E

S

D

N

E

R

G

T

G

L

W

G

-

V

I

Q

F

Q

I

N

D

G

G

G

M

L

Q

A

A

E

E

R

Y

I

L

V

R

L

V

P

P

Y

H

G

A

K

D

L

N

I

T

L

L

N

Y

D

H

T

T

L

-

A

-

P

P

R

E

H

D

L

L

-

S

-

D

-

E

A

A

L

L

V

V

E

M

P

L

L

L

S

P

V

T

G

G

F

Y

H

E

A

I

A

G

A

V

-

L

R

K

G

G

R

K

V

V

E

E

A

P

A

G

D

C

T

S

V

V

L

A

V

I

L

I

G

G

C

S

G

G

M

P

I

V

G

G

M

L

G

A

A

A

I

L

-

L

A

T

G

A

S

Q

V

F

H

Y

R

S

G

P

A

A

T

K

V

L

I

I

A

M

V

V

D

D

I

L

G

D

E

D

A

N

K

R

T

L

G

E

L

T

A

A

R

L

R

S

Y

F

G

G

A

A

A

T

H

H

T

K

I

V

D

N

A

S

G

S

K

D

E

P

D

E

V

K

A

A

A

I

R

K

-

E

I

I

A

Y

E

D

L

L

T

T

N

D

G

G

D

R

G

G

A

V

D

D

V

V

V

A

I

I

E

E

A

A

V

V

G

G

L

I

P

P

A

A

T

T

F

F

T

D

Q

F

A

C

I

Q

D

K

L

I

V

I

S

G

F

V

A

D

G

G

R

T

V

V

V

A

Y

N

I

C

G

G

Y

V

A

H

K

G

E

K

P

P

V

V

S

E

Y

F

K

D

T

L

Q

D

F

K

F

L

N

W

L

I

K

R

E

N

L

I

D

N

I

V

-

T

-

G

M

L

G

V

S

S

R

T

N

N

A

T

T

T

S

P

E

Q

D

L

F

L

Q

K

A

A

V

-

I

-

H

-

Y

-

L

L

E

E

T

S

L

H

D

K

H

I

A

E

P

P

D

E

D

K

L

L

I

V

S

T

K

H

V

Y

F

F

P

K

F

L

E

S

E

E

A

I

D

E

K

K

A

A

L

Y

P

E

Y

V

W

F

A

S

K

K

E

A

R

A

D

D

T

H

V

A

K

K

V

V

M

I

I

I

E

E

R

N

active site: C41 (= C33), T43 (≠ S35), H46 (≠ S38), H63 (= H55)
binding zinc ion: C41 (= C33), H63 (= H55), C93 (= C86), C96 (= C89), C99 (= C92), C107 (= C100)

2eerB Structural study of project id st2577 from sulfolobus tokodaii strain7
29% identity, 92% coverage: 10:317/334 of query aligns to 15:331/347 of 2eerB

query
sites
2eerB

I

L

H

E

E

D

V

I

A

P

E

I

P

P

T

K

P

P

G

K

P

G

G

S

E

Q

V

V

L

L

L

I

A

K

I

I

R

E

H

A

V

A

A

G

L

V

C
|
C

G
x
H

S
|
S

D

D

L

V

S
x
H

T

M

F

R

K

Q

G

G

-

R

-

F

-

G

-

N

-

L

-

R

-

I

L

V

N

E

P

D

L

L

-

G

V

V

S

K

L

L

P

P

R

V

I

T

P

L

G

G

H
|
H

E
|
E

I

I

G

A

G

G

E

R

I

I

A

E

V

V

G

G

E

D

G

E

V

V

P

V

A

G

E

-

Y

Y

T

S

V

K

G

G

R

D

R

L

A

V

I

A

V

V

I

N

P

P

Y

W

T

E

A

G

C
x
E

G

G

T

N

C
|
C

P

Y

S

Y

C
|
C

R

R

K

I

G

G

R

E

V

E

N

H

A

L

C
|
C

R

D

S

S

N

P

R
|
R

T

W

L

L

G

G

V

I

Q

N

Q

Y

N

D

G

G

G

A

L

Y

A

A

E

E

R

Y

I

V

V

L

L

V

P

P

Y

H

G

Y

K

K

L

Y

I

L

L

Y

N

K

D

-

T

-

L

-

A

-

P

L

R

R

H

R

L

L

A

S

L

A

V

V

E

E

-

A

P

P

L

L

S

T

V
x
C

G

S

-

G

-

V

-
x
T

-

T

F

Y

H

R

A

A

A

V

A

R

R

K

G

A

R

S

V

L

E

D

A

P

A

S

D

K

T

T

V

L

L

V

V

V

L
x
I

G
|
G

C

A

G
|
G

M
x
G

I

-

G
|
G

M
x
L

G

G

A

T

I

M

A

A

G

I

S

Q

V

I

H

A

R

K

-

A

-

V

-

S

G

G

A

A

T

T

V

I

I

I

A

G

V
|
V

D
|
D

I
x
V

G
x
R

E

E

A

E

K

A

T

L

G

E

L

A

A

A

R

K

R

R

Y

A

G

G

A

A

A

D

H

Y

T

V

I

I

D

N

A

A

G

S

K

S

E

Q

D

D

V

P

A

V

A

S

R

E

I

I

A

R

E

R

L

I

T

T

N

Q

G

G

D

K

G

G

A

A

D

D

V

A

V

V

I

I

E

D

A
x
L

V
x
N

G

N

L

S

P

E

A
x
K

T
|
T

F

L

T

S

Q

I

A

Y

I

P

D

Y

L

V

V

L

S

A

F

K

A

Q

G

G

R

K

V

Y

V

V

Y

M

I
x
V

G
|
G

Y
x
L

A
x
F

K

G

E

A

P

D

V

L

S

K

Y

Y

K

H

T

A

Q

P

F

L

F

I

N

T

L

L

K

N

E

E

L

V

D

Q

I

F

M

I

G

G

S
|
S

R
x
L

N
x
V

A

G

T

N

S

Q

E

S

D

D

F

F

Q

L

A

G

V

I

I

M

H

S

Y

L

L

A

E

E

T

A

L

G

D

K

H

V

A

K

P

P

D

-

L

M

I

V

S

T

K

K

V

T

F

M

P

K

F

L

E

E

A

A

D

N

K

E

A

A

L

I

active site: C38 (= C33), H39 (≠ G34), S40 (= S35), H43 (≠ S38), H68 (= H55), E69 (= E56), E98 (≠ C86), C101 (= C89), C104 (= C92), C112 (= C100), R116 (= R104), C154 (≠ V144), T158 (vs. gap)
binding nicotinamide-adenine-dinucleotide: C38 (= C33), H39 (≠ G34), S40 (= S35), H43 (≠ S38), C154 (≠ V144), T158 (vs. gap), I177 (≠ L163), G178 (= G164), G180 (= G166), G181 (≠ M167), G182 (= G169), L183 (≠ M170), V202 (= V186), D203 (= D187), V204 (≠ I188), R205 (≠ G189), L247 (≠ A231), N248 (≠ V232), K252 (≠ A236), T253 (= T237), V270 (≠ I254), G271 (= G255), L272 (≠ Y256), F273 (≠ A257), S294 (= S278), L295 (≠ R279), V296 (≠ N280)
binding zinc ion: E98 (≠ C86), C101 (= C89), C104 (= C92), C112 (= C100)

Sites not aligning to the query:

active site: 342
binding nicotinamide-adenine-dinucleotide: 334, 337, 342

Q5JI69 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see paper)
31% identity, 80% coverage: 12:277/334 of query aligns to 21:290/350 of Q5JI69

query
sites
Q5JI69

E

E

V

V

A

D

E

V

P

P

T

K

P

P

G

G

P

P

G

G

E

E

V

V

L

L

L

I

A

K

I

V

R

L

H

A

V

T

A

S

L

I

C

C

G

G

S

T

D

D

L

L

S

H

T

I

F

Y

K

E

G

W

-

N

-

E

-

W

L

A

N

Q

P

S

L

R

V

I

S

K

L

P

P

P

R

Q

I

I

P

M

G

G

H

H

E

E

I

V

G

A

G

G

E

E

I

V

A

V

A

E

V

V

G

G

E

P

G

G

V

V

P

-

A

E

E

D

Y

L

T

Q

V

V

G

G

R

D

R

Y

A

I

I

S

V

V

I

E

P

T

Y

H

T

I

A

V

C

C

G

G

T

K

C

C

P

Y

S

A

C

C

R

K

K

H

G

N

R

R

V

Y

N

H

A

V

C

C

R

Q

S

N

N

T

R

K

T

I

L

F

G

G

V

V

Q

D

Q

M

N

D

G

G

G

V

L

F

A

A

E

H

R

Y

I

A

V

I

L

V

P

P

Y

A

G

K

K

N

L

A

I

W

L

K

N

N

-

P

D

K

T

D

L

M

A

P

P

P

R

E

H

Y

L

A

A

A

L

L

V

Q

E

E

P

P

L

L

S

G

V

N

G

A

F

V

H

D

A

T

A

V

A

L

R

A

G

G

R

P

V

I

E

-

A

A

A

G

D

R

T

S

V

T

L

L

V

I

L

T

G

G

C

A

G

G

M

P

I
x
L

G

G

M

L

G

L

A

G

I

I

A

A

G

V

S

A

V

K

H

A

R

S

G

G

A

A

-

Y

T

P

V

V

I

I

A

V

V

S

D
x
E

I

P

G

S

E

E

A

F

K
x
R

T

R

G

K

L

L

A

A

R

K

Y

V

G

G

A

A

A

D

H

Y

T

V

I

V

D

N

A

P

G

F

K

E

E

E

D

D

V

P

A

V

A

K

R

F

I

V

A

M

E

D

L

I

T

T

N

D

G

G

D

A

G

G

A

V

D

E

V

V

V

F

I

L

E

E

A

F

V

S

G

G

L

A

P

P

A

K

T

A

F

L

T

E

Q

Q

A

G

I

L

D

K

L

A

V

V

S

T

F

P

A

G

G

G

R

R

V

V

V

S

Y

L

I
x
L

G
|
G

Y
x
L

A

F

K

P

E

R

P

E

V

V

S

T

Y

-

K

-

T

I

Q

D

F

F

F

N

N

N

L

L

-

I

-

F

K

K

E

A

L

L

D

E

I

V

M

H

G

G

L179 (≠ I168) binding
E199 (≠ D187) binding
R204 (≠ K192) binding
LGL 266:268 (≠ IGY 254:256) binding

Sites not aligning to the query:

291:292 binding

3gfbA L-threonine dehydrogenase (tktdh) from the hyperthermophilic archaeon thermococcus kodakaraensis (see paper)
31% identity, 80% coverage: 12:277/334 of query aligns to 19:288/347 of 3gfbA

query
sites
3gfbA

E

E

V

V

A

D

E

V

P

P

T

K

P

P

G

G

P

P

G

G

E

E

V

V

L

L

L

I

A

K

I

V

R

L

H

A

V

T

A

S

L

I

C
|
C

G
|
G

S
x
T

D

D

L

L

S
x
H

T

I

F

Y

K

E

G

W

-

N

-

E

-

W

L

A

N

Q

P

S

L

R

V

I

S

K

L

P

P

P

R

Q

I

I

P

M

G

G

H
|
H

E
|
E

I

V

G

A

G

G

E

E

I

V

A

V

A

E

V

V

G

G

E

P

G

G

V

V

P

-

A

E

E

D

Y

L

T

Q

V

V

G

G

R

D

R

Y

A

I

I

S

V

V

I

E

P

T

Y

H

T

I

A

V

C
|
C

G

G

T

K

C
|
C

P

Y

S

A

C
|
C

R

K

K

H

G

N

R

R

V

Y

N

H

A

V

C
|
C

R

Q

S

N

N

T

R
x
K

T

I

L

F

G

G

V

V

Q

D

Q

M

N

D

G

G

G

V

L

F

A

A

E

H

R

Y

I

A

V

I

L

V

P

P

Y

A

G

K

K

N

L

A

I

W

L

K

N

N

-

P

D

K

T

D

L

M

A

P

P

P

R

E

H

Y

L

A

A

A

L

L

V

Q

E

E

P
|
P

L

L

S

G

V

N

G
x
A

F

V

H

D

A

T

A

V

A

L

R

A

G

G

R

P

V

I

E

-

A

A

A

G

D

R

T

S

V

T

L

L

V

I

L

T

G
|
G

C
x
A

G
|
G

M
x
P

I
x
L

G

G

M

L

G

L

A

G

I

I

A

A

G

V

S

A

V

K

H

A

R

S

G

G

A

A

-

Y

T

P

V

V

I

I

A

V

V
x
S

D
x
E

I
x
P

G

S

E

E

A

F

K
x
R

T

R

G

K

L

L

A

A

R

K

Y

V

G

G

A

A

A

D

H

Y

T

V

I

V

D

N

A
x
P

G

F

K

E

E

E

D

D

V

P

A

V

A

K

R

F

I

V

A

M

E

D

L

I

T

T

N

D

G

G

D

A

G

G

A

V

D

E

V

V

V

F

I

L

E

E

A
x
F

V
x
S

G

G

L
x
A

P

P

A

K

T
x
A

F

L

T

E

Q

Q

A

G

I

L

D

K

L

A

V

V

S

T

F

P

A

G

G

G

R

R

V

V

V

S

Y

L

I
x
L

G
|
G

Y
x
L

A

F

K

P

E

R

P

E

V

V

S

T

Y

-

K

-

T

I

Q

D

F

F

F

N

N

N

L

L

-

I

-
x
I

-
x
F

K

K

E

A

L

L

D

E

I

V

M

H

G

G

active site: C40 (= C33), G41 (= G34), T42 (≠ S35), H45 (≠ S38), H65 (= H55), E66 (= E56), C95 (= C86), C98 (= C89), C101 (= C92), C109 (= C100), K113 (≠ R104), P151 (= P141), A155 (≠ G145)
binding nicotinamide-adenine-dinucleotide: G173 (= G164), A174 (≠ C165), G175 (= G166), P176 (≠ M167), L177 (≠ I168), S196 (≠ V186), E197 (≠ D187), P198 (≠ I188), R202 (≠ K192), P217 (≠ A207), F241 (≠ A231), S242 (≠ V232), A244 (≠ L234), A247 (≠ T237), L264 (≠ I254), G265 (= G255), L266 (≠ Y256), I280 (vs. gap), F281 (vs. gap)

Sites not aligning to the query:

active site: 340
binding nicotinamide-adenine-dinucleotide: 289, 290

5kiaA Crystal structure of l-threonine 3-dehydrogenase from burkholderia thailandensis
28% identity, 91% coverage: 13:317/334 of query aligns to 17:323/339 of 5kiaA

query
sites
5kiaA

V

V

A

K

E

K

P

P

T

E

P

V

G

G

P

H

G

N

E

D

V

V

L

L

L

I

A

K

I

I

R

R

H

R

V

T

A

A

L

I

C
|
C

G
|
G

S
x
T

D

D

L

I

S
x
H

T

I

F

W

K

K

G

W

L

D

N

D

P

-

L

-

V

W

S

A

L

Q

P

K

R

T

I

I

P

P

-

V

-

M

-

H

-

V

G

G

H
|
H

E
|
E

I

Y

G

V

G

G

E

E

I

I

A

V

A

E

V

M

G

G

E

Q

G

E

V

V

P

R

A

G

E

-

Y

F

T

S

V

I

G

G

R

D

R

R

A

V

I

S

V

G

I

E

P

G

Y

H

T

I

A

T

C
|
C

G

G

T

F

C
|
C

P

R

S

N

C
|
C

R

R

K

A

G

G

R

R

V

R

N

H

A

L

C
|
C

R

R

S

N

N

T

R
x
V

T

G

L

V

G

G

V

V

Q

N

Q

R

N

E

G

G

G

A

L

F

A

A

E

E

R

Y

I

L

V

A

L

I

P

P

-

A

Y

F

G

N

K

A

L

F

I

K

L

I

N

P

D

P

T

E

L

I

A

S

P

D

R

D

H

L

L

A

A

A

L

I

V

F

E
x
D

P
|
P

L

F

S

G

V
x
N

G
x
A

F

T

H

H

A

T

A

A

A

L

R

S

G

F

R

N

V

L

E

V

A

G

A

E

D

D

T

-

V

V

L

L

V

I

L

T

G

G

C

A

G

G

M

P

I

I

G

G

M

V

G

M

A

A

I

V

A

A

G

I

S

A

V

K

H

H

R

V

G

G

A

A

-

R

T

N

V

V

I

V

A

I

V

T

D

D

I

I

G

N

E

D

A

Y

K

R

T

L

G

D

L

L

A

A

R

R

Y

M

G

G

A

A

A

T

H

R

T

A

I

V

D

N

A

V

G

S

K

R

E

E

D

S

V

L

A

R

A

D

R

V

I

M

A

A

E

D

L

L

T

H

N

M

G

T

D

E

G

G

A

F

D

D

V

V

V

G

I

L

E

E

A

M

V

S

G

G

L

V

P

P

A

S

T

A

F

F

T

T

Q

S

A

L

I

L

D

E

L

S

V

M

S

N

F

H

A

G

G

G

R

K

V

V

V

A

Y

L

I

L

G

G

Y

I

-

P

A

A

K

Q

E

T

P

A

V

I

S

D

Y

W

K

N

T

Q

Q

V

F

I

F

F

N

K

L

G

K

L

E

E

L

I

D

K

I

G

M

I

G

Y

S

G

R

R

N
x
E

A

M

T

F

S

E

E

T

D

W

F

Y

Q

K

A

M

V

V

I

A

H

M

Y

L

L

Q

E

S

T

G

L

L

D

D

H

L

A

S

P

P

D

-

L

I

I

I

S

T

K

H

V

R

F

F

P

A

F

V

E

D

A

Y

D

E

K

K

A

G

L

F

active site: C37 (= C33), G38 (= G34), T39 (≠ S35), H42 (≠ S38), H61 (= H55), E62 (= E56), C91 (= C86), C94 (= C89), C97 (= C92), C105 (= C100), V109 (≠ R104), P147 (= P141), A151 (≠ G145)
binding calcium ion: D146 (≠ E140), N150 (≠ V144), E288 (≠ N280)
binding zinc ion: C91 (= C86), C94 (= C89), C97 (= C92), C105 (= C100)

Sites not aligning to the query:

active site: 333

P07913 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Escherichia coli (strain K12) (see paper)
28% identity, 92% coverage: 12:317/334 of query aligns to 17:325/341 of P07913

query
sites
P07913

E

D

V

V

A

P

E

V

P

P

T

E

P

L

G

G

P

H

G

N

E

D

V

L

L

L

L

I

A

K

I

I

R

R

H

K

V

T

A

A

L

I

C
|
C

G

G

S

T

D

D

L

V

S

H

T

I

F

Y

-

N

-

W

-

D

K

E

G

W

L

S

N

Q

P

K

L

T

V

I

S

P

L

V

P

P

R

M

I

V

P

V

G

G

H

H

E

E

I

Y

G

V

G

G

E

E

I

V

A

V

A

G

V

I

G

G

E

Q

G

E

V

V

P

K

A

G

E

-

Y

F

T

K

V

I

G

G

R

D

R

R

A

V

I

S

V

G

I

E

P

G

Y

H

T

I

A

T

C

C

G

G

T

H

C

C

P

R

S

N

C

C

R

R

K

G

G

G

R

R

V

T

N

H

A

L

C

C

R

R

S

N

N

T

R

I

T

G

L

V

G

G

V

V

Q

N

Q

R

N

P

G

G

G

C

L

F

A

A

E

E

R

Y

I

L

V

V

L

I

P

P

-

A

Y

F

G

N

K

A

L

F

I

K

L

I

N

P

D

D

T

N

L

I

A

S

P

D

R

D

H

L

L

A

A

A

L

I

V

F

E

D

P

P

L

F

S

G

V

N

G

A

F

V

H

H

A

T

A

A

A

L

R

S

G

F

R

D

V

L

E

V

A

G

A

E

D

D

T

-

V

V

L

L

V

V

L

S

G

G

C

A

G

G

M

P

I

I

G

G

M

I

G

M

A

A

I

A

A

A

G

V

S

A

V

K

H

H

R

V

G

G

A

A

-

R

T

N

V

V

I

V

A

I

V

T

D

D

I

V

G

N

E

E

A

Y

K

R

T

L

G

E

L

L

A

A

R

R

R

K

Y

M

G

G

A

I

A

T

H

R

T

A

I

V

D

N

A

V

G

A

K

K

E

E

D

N

V

L

A

N

A

D

R

V

I

M

A

A

E

E

L

L

T

G

N

M

G

T

D

E

G

G

A

F

D

D

V

V

V

G

I

L

E

E

A

M

V

S

G

G

L

A

P

P

A

P

T

A

F

F

T

R

Q

T

A

M

I

L

D

D

L

T

V

M

S

N

F

H

A

G

G

G

R

R

V

I

V

A

Y

M

I

L

G

G

Y

I

A

P

K

P

E

S

P

D

V

M

S

S

Y

I

K

D

T

W

Q

T

F

K

F

V

N

I

L

F

K

K

E

G

L

L

D

F

I

I

M

K

G

G

-

I

-

Y

S

G

R

R

N

E

A

M

T

F

S

E

E

T

D

W

F

Y

Q

K

A

M

V

A

I

A

H

L

Y

I

L

Q

E

S

T

G

L

L

D

D

H

L

A

S

P

P

D

-

L

I

I

I

S

T

K

H

V

R

F

F

P

S

F

I

E

D

A

F

D

Q

K

K

A

G

L

F

C38 (= C33) mutation to D: Shows only 1% of wild-type catalytic activity. This mutant can be stimulated to the wild-type activity level after incubation with Zn(+).; mutation to S: Loss of catalytic activity. This mutant cannot be stimulated to the wild-type activity level after incubation with Zn(+).

5vm2A Crystal structure of eck1772, an oxidoreductase/dehydrogenase of unknown specificity involved in membrane biogenesis from escherichia coli
28% identity, 95% coverage: 16:333/334 of query aligns to 22:344/347 of 5vm2A

query
sites
5vm2A

P

P

T

V

P

P

G

K

P

E

G

D

E

E

V

V

L

L

L

I

A

K

I

V

R

E

H

Y

V

V

A

G

L

I

C
|
C

G
|
G

S
|
S

D

D

L

V

S
x
H

T

G

F

F

K

E

G

S

L

-

N

G

P

P

L

F

V

I

S

P

L

-

P

P

R

K

I

D

P

P

-

N

-

Q

-

E

-

I

-

G

-

L

G

G

H
|
H

E
|
E

I

C

G

A

G

G

E

T

I

V

A

V

A

A

V

V

G

G

E

S

G

R

V

V

P

-

A

R

E

K

Y

F

T

K

V

P

G

G

R

D

R

R

A

V

I

N

V

I

I

E

P

P

Y

G

T

V

A

P

C
|
C

G

G

T

H

C
|
C

P

R

S

Y

C
|
C

R

L

K

E

G

G

R

K

V

Y

N

N

A

I

C
|
C

R

P

S

D

N

V

R
x
D

T

F

L

M

G

A

V

T

Q

Q

Q

P

N

N

-

Y

-

R

G

G

G

A

L

L

A

T

E

H

R

Y

I

L

V

C

L

H

P

P

Y

E

G

S

-

F

K

T

L

Y

I

K

L

L

N

P

D

D

T

N

L

M

A

D

P

T

R

M

H

E

L

G

A

T

L

L

V

V

E
|
E

P
|
P

L

A

S

A

V

V

G
|
G

F

M

H

H

A

A

A

M

R

L

G

A

R

D

V

V

E

K

A

P

A

G

D

K

T

K

V

I

L

I

V

I

L

L

G

G

C

A

G

G

M

C

I

I

G

G

M

L

G

M

A

T

I

L

A

Q

G

A

S

C

V

K

H

C

R

L

G

G

A

A

T

T

V

E

I

I

A

A

V

V

-

V

D

D

I

V

G

L

E

E

A

K

K

R

T

L

G

A

L

M

A

A

R

E

R

Q

Y

L

G

G

A

A

A

T

H

V

T

V

I

I

D

N

A

G

G

A

K

K

E

E

D

D

V

T

A

I

A

A

R

R

I

C

A

Q

E

Q

L

F

T

T

N

E

G

D

D

M

G

G

A

A

D

D

V

I

V

V

I

F

E

E

A

T

V

A

G

G

L

S

P

A

A

V

T

T

F

V

T

K

Q

Q

A

A

I

P

D

Y

L

L

V

V

S

M

F

R

A

G

G

G

R

K

V

I

V

M

Y

I

I

V

G

G

Y

T

A

V

K

-

E

-

P

P

V

G

S

A

Y

S

K

A

T

I

Q

N

F

F

F

L

N

K

L

I

K

N

E

R

L

E

D

V

I

T

M

I

G

Q

S

T

R

V

N

F

A

R

T

Y

S

A

E

N

D

R

F

Y

Q

P

A

V

V

T

I

I

H

E

Y

A

L

I

E

S

T

S

L

G

D

R

H

F

A

D

P

V

D

K

D

S

L

M

I

V

S

T

K

H

V

I

F

Y

P

D

F

Y

E

R

E

D

A

V

D

Q

K

Q

A

A

L

F

P

E

Y

E

W

S

A

V

K

N

E

N

R

K

D

R

T

D

T

I

V

I

K
|
K

V

G

M

V

I

I

E

K

active site: C39 (= C33), G40 (= G34), S41 (= S35), H44 (≠ S38), H65 (= H55), E66 (= E56), C95 (= C86), C98 (= C89), C101 (= C92), C109 (= C100), D113 (≠ R104), P153 (= P141), G157 (= G145), K340 (= K329)
binding magnesium ion: H65 (= H55), E66 (= E56), E152 (= E140)
binding zinc ion: C95 (= C86), C98 (= C89), C101 (= C92), C109 (= C100)

7y9pA Xylitol dehydrogenase s96c/s99c/y102c mutant(thermostabilized form) from pichia stipitis (see paper)
31% identity, 91% coverage: 12:316/334 of query aligns to 23:339/357 of 7y9pA

query
sites
7y9pA

E

E

V

I

A

S

E

E

P

P

T

T

P

-

G

-

P

-

G

-

E

D

V

V

L

L

L

V

A

Q

I

V

R

K

H

K

V

T

A

G

L

I

C
|
C

G

G

S

S

D

D

L

I

S

H

T

F

F

Y

-

A

-

H

-

G

K

R

G

I

L

G

N

N

P

F

L

V

V

L

S

T

L

K

P

P

R

M

I

V

P

L

G

G

H
|
H

E
|
E

I

S

G

A

G

G

E

T

I

V

A

V

A

Q

V

V

G

G

E

K

G

G

V

V

P

-

A

T

E

S

Y

L

T

K

V

V

G

G

R

D

R

N

A

V

I

A

V

I

I

E

P

P

Y

G

T

I

A

P

C
|