SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AZOBR_RS16425 FitnessBrowser__azobra:AZOBR_RS16425 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
46% identity, 92% coverage: 8:274/290 of query aligns to 4:274/306 of 4whxA

query
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4whxA

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active site: F37 (= F41), K160 (= K159), E194 (= E193), L217 (= L216)
binding alanine: Y97 (= Y96), K160 (= K159), A258 (= A257)

2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
42% identity, 89% coverage: 9:266/290 of query aligns to 3:265/305 of 2ej0B

query
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2ej0B

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active site: F35 (= F41), G37 (= G43), K158 (= K159), E192 (= E193), L215 (= L216)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R60), Y163 (= Y164), E192 (= E193), G195 (= G196), E196 (≠ A197), L215 (= L216), G217 (= G218), I218 (= I219), T219 (= T220), G254 (= G255), T255 (= T256)

1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
42% identity, 90% coverage: 13:273/290 of query aligns to 5:276/304 of 1iyeA

query
sites
1iyeA

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active site: F33 (= F41), G35 (= G43), K156 (= K159), A157 (= A160), E190 (= E193), L214 (= L216)
binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R60), Y92 (= Y96), Y126 (= Y129), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (≠ A197), N195 (= N198), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256), A255 (= A257)

1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
42% identity, 90% coverage: 13:273/290 of query aligns to 5:276/304 of 1iydA

query
sites
1iydA

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active site: F33 (= F41), G35 (= G43), K156 (= K159), A157 (= A160), E190 (= E193), L214 (= L216)
binding glutaric acid: Y92 (= Y96), Y126 (= Y129), A255 (= A257)
binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (≠ A197), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), T254 (= T256)

1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
42% identity, 90% coverage: 13:273/290 of query aligns to 5:276/304 of 1i1mA

query
sites
1i1mA

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G

M

M

G

G

V

V

A

N

A

P

Q

P

A

A

S

G

-

Y

R

S

I

T

H

D

V

V

A

I

I

I

A

A

V

A

W

F

Q

P

W

W

P

G

S

A

Y

Y

F

L

S

G

P

A

E

E

A

A

K

L

M

E

A

Q

G

G

I

I

K

D

L

A

T

M

W

V

S

S

R

S

W

W

R

N

R

R

P

A

A

A

P

P

D

N

M

T

A

I

P

P

T

T

A

A

S

A

K
|
K

A

A

A

G

G

G

L

N

Y
|
Y

M

L

I

S

C

S

T

L

L

L

S

V

K

G

H

S

E

E

A

A

E

R

A

R

E

H

G

G

Y

Y

Q

Q

D

E

A

G

L

I

M

A

L

L

D

D

Y

V

R

N

G

G

Y

Y

L

I

A

S

E
|
E

A

G

T

A

G
|
G

A
x
E

N

N

L

L

F

F

L

E

V

V

M

K

D

D

G

G

K

V

I

L

H

F

T

T

P

P

K

P

-

F

P

T

D

S

C

S

F

A

L
|
L

D

P

G
|
G

I
|
I

T
|
T

R

R

R

D

T

A

V

I

I

I

D

K

L

L

A

A

K

K

A

E

R

L

G

G

I

I

E

E

V

V

I

R

E

E

R

Q

H

V

I

L

Q

S

P

R

D

E

E

S

L

L

A

Y

N

L

T

A

Q

D

E

E

V

V

F

F

L

M

T

S

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

V

V

G

R

-

S

-

V

-

D

-

G

-

I

Q

Q

I

V

G

G

D

E

H

G

R

R

F

C

T

G

P

P

active site: K156 (= K159)
binding 4-methyl valeric acid: Y92 (= Y96), K156 (= K159), T254 (= T256), A255 (= A257)
binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (≠ A197), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256)

1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
42% identity, 90% coverage: 13:273/290 of query aligns to 5:276/304 of 1i1lA

query
sites
1i1lA

I

I

W

W

Y

F

D

N

G

G

A

E

L

M

V

V

P

R

W

W

R

E

D

D

A

A

N

K

L

V

H

H

V

V

L

M

S

S

H

H

G

A

L

L

H

H

Y

Y

A

G

S

T

C

S

V

V

F

F

E

E

G

G

E

I

R

R

V

C

Y

Y

N

D

G

S

-

H

-

K

-

G

-

P

T

V

V

V

F

F

K

R

L

H

T

R

E

E

H

H

S

M

E

Q

R
|
R

L

L

A

H

A

D

S

S

A

A

R

K

I

I

L

Y

G

R

F

F

E

P

L

V

P

S

Y

Q

S

S

V

I

A

D

E

E

I

L

D

M

A

E

A

A

T

C

N

R

E

D

T

V

V

I

K

R

A

K

M

N

G

N

F

L

T

T

D

S

A

A

Y
|
Y

V

I

R

R

P

P

V

L

A

I

W

F

R

V

G

G

S

D

E

V

M

G

M

M

G

G

V

V

A

N

A

P

Q

P

A

A

S

G

-

Y

R

S

I

T

H

D

V

V

A

I

I

I

A

A

V

A

W

F

Q

P

W

W

P

G

S

A

Y

Y

F

L

S

G

P

A

E

E

A

A

K

L

M

E

A

Q

G

G

I

I

K

D

L

A

T

M

W

V

S

S

R

S

W

W

R

N

R

R

P

A

A

A

P

P

D

N

M

T

A

I

P

P

T

T

A

A

S

A

K
|
K

A

A

A

G

G

G

L

N

Y
|
Y

M

L

I

S

C

S

T

L

L

L

S

V

K

G

H

S

E

E

A

A

E

R

A

R

E

H

G

G

Y

Y

Q

Q

D

E

A

G

L

I

M

A

L

L

D

D

Y

V

R

N

G

G

Y

Y

L

I

A

S

E
|
E

A

G

T

A

G
|
G

A

E

N

N

L

L

F

F

L

E

V

V

M

K

D

D

G

G

K

V

I

L

H

F

T

T

P

P

K

P

-

F

P

T

D

S

C

S

F

A

L

L

D

P

G
|
G

I
|
I

T
|
T

R

R

R

D

T

A

V

I

I

I

D

K

L

L

A

A

K

K

A

E

R

L

G

G

I

I

E

E

V

V

I

R

E

E

R

Q

H

V

I

L

Q

S

P

R

D

E

E

S

L

L

A

Y

N

L

T

A

Q

D

E

E

V

V

F

F

L

M

T

S

G

G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

V

V

G

R

-

S

-

V

-

D

-

G

-

I

Q

Q

I

V

G

G

D

E

H

G

R

R

F

C

T

G

P

P

active site: K156 (= K159)
binding 2-methylleucine: Y92 (= Y96), K156 (= K159), T254 (= T256), A255 (= A257)
binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), G216 (= G218), I217 (= I219), T218 (= T220), T254 (= T256)

2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
41% identity, 89% coverage: 9:266/290 of query aligns to 3:257/297 of 2ej3A

query
sites
2ej3A

R

K

D

A

G

G

V

L

I

I

W

W

Y

M

D

N

G

G

A

A

L

F

V

V

P

P

W

Q

R

E

D

E

A

A

N

K

L

T

H

S

V

V

L

L

S

S

H

H

G

A

L

L

H

H

Y

Y

A

G

S

T

C

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

N

E

G

T

T

A

-

K

-

G

-

P

-

A

V

I

F

F

K

R

L

L

T

K

E

E

H

H

S

V

E

K

R
|
R

L

F

A

Y

A

N

S

S

A

A

R

K

I

V

L

L

G

R

F

M

E

E

L

I

P

P

Y

F

S

A

V

P

A

E

E

E

I

L

D

E

A

E

A

A

T

I

N

K

E

E

T

V

V

V

K

R

A

R

M

N

G

G

F

Y

T

R

D

S

A

C

Y

Y

V

I

R

R

P

P

V

L

A

A

W

W

R

M

G

G

S

A

E

K

M

A

M

L

G

G

V

V

A

N

A

P

Q

L

A

P

S

N

R

N

-

P

I

A

H

E

V

V

A

M

I

V

A

A

V

A

W

W

Q

E

W

W

P

V

S

R

Y

-

F

-

S

-

P

-

E

-

A

-

K

-

M

-

A

K

G

G

I

A

K

R

L

L

T

I

W

T

S

S

R

S

W

W

R

A

R

R

P

F

A

P

P

A

D

N

M

V

A

M

P

P

T

G

A

K

S

A

K
|
K

A

V

A

G

G

G

L

N

Y
|
Y

M

V

I

N

C

S

T

A

L

L

S

A

K

K

H

M

E

E

A

A

E

V

A

A

E

A

G

G

Y

A

Q

D

D

E

A

A

L

L

M

L

L

L

D

D

Y

E

R

E

G

G

Y

Y

L

V

A

A

E
|
E

A

G

T

S

G
|
G

A

E

N

N

L

L

F

F

L

F

V

V

M

R

D

D

G

G

K

V

I

I

H

Y

T

A

P

L

K

E

P

H

D

S

C

V

F

N

L
|
L

D

E

G
|
G

I
|
I

T
|
T

R

R

R

D

T

S

V

V

I

I

D

R

L

I

A

A

K

K

A

D

R

L

G

G

I

Y

E

E

V

V

I

Q

E

V

R

V

H

R

I

A

Q

T

P

R

D

D

E

Q

L

L

A

Y

N

M

T

A

Q

D

E

E

V

V

F

F

L

M

T

T

G
|
G

T
|
T

A
|
A

A

A

E

E

V

V

T

T

P

P

V

V

G

S

Q

M

I

I

active site: F35 (= F41), G37 (= G43), K150 (= K159), E184 (= E193), L207 (= L216)
binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (= G196), G246 (= G255), T247 (= T256), A248 (= A257)
binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), G246 (= G255), T247 (= T256)

2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
41% identity, 89% coverage: 9:266/290 of query aligns to 3:257/297 of 2eiyA

query
sites
2eiyA

R

K

D

A

G

G

V

L

I

I

W

W

Y

M

D

N

G

G

A

A

L

F

V

V

P

P

W

Q

R

E

D

E

A

A

N

K

L

T

H

S

V

V

L

L

S

S

H

H

G

A

L

L

H

H

Y

Y

A

G

S

T

C

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

N

E

G

T

T

A

-

K

-

G

-

P

-

A

V

I

F

F

K

R

L

L

T

K

E

E

H

H

S

V

E

K

R
|
R

L

F

A

Y

A

N

S

S

A

A

R

K

I

V

L

L

G

R

F

M

E

E

L

I

P

P

Y

F

S

A

V

P

A

E

E

E

I

L

D

E

A

E

A

A

T

I

N

K

E

E

T

V

V

V

K

R

A

R

M

N

G

G

F

Y

T

R

D

S

A

C

Y
|
Y

V

I

R

R

P

P

V

L

A

A

W

W

R

M

G

G

S

A

E

K

M

A

M

L

G

G

V

V

A

N

A

P

Q

L

A

P

S

N

R

N

-

P

I

A

H

E

V

V

A

M

I

V

A

A

V

A

W

W

Q

E

W

W

P

V

S

R

Y

-

F

-

S

-

P

-

E

-

A

-

K

-

M

-

A

K

G

G

I

A

K

R

L

L

T

I

W

T

S

S

R

S

W

W

R

A

R

R

P

F

A

P

P

A

D

N

M

V

A

M

P

P

T

G

A

K

S

A

K
|
K

A

V

A

G

G

G

L

N

Y
|
Y

M

V

I

N

C

S

T

A

L

L

S

A

K

K

H

M

E

E

A

A

E

V

A

A

E

A

G

G

Y

A

Q

D

D

E

A

A

L

L

M

L

L

L

D

D

Y

E

R

E

G

G

Y

Y

L

V

A

A

E
|
E

A

G

T

S

G
|
G

A
x
E

N

N

L

L

F

F

L

F

V

V

M

R

D

D

G

G

K

V

I

I

H

Y

T

A

P

L

K

E

P

H

D

S

C

V

F

N

L
|
L

D

E

G
|
G

I
|
I

T
|
T

R

R

R

D

T

S

V

V

I

I

D

R

L

I

A

A

K

K

A

D

R

L

G

G

I

Y

E

E

V

V

I

Q

E

V

R

V

H

R

I

A

Q

T

P

R

D

D

E

Q

L

L

A

Y

N

M

T

A

Q

D

E

E

V

V

F

F

L

M

T

T

G
|
G

T
|
T

A
|
A

A

A

E

E

V

V

T

T

P

P

V

V

G

S

Q

M

I

I

active site: F35 (= F41), G37 (= G43), K150 (= K159), E184 (= E193), L207 (= L216)
binding 4-methyl valeric acid: F35 (= F41), Y94 (= Y96), T247 (= T256), A248 (= A257)
binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), E188 (≠ A197), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), G246 (= G255), T247 (= T256)

1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
41% identity, 89% coverage: 9:266/290 of query aligns to 3:257/297 of 1wrvA

query
sites
1wrvA

R

K

D

A

G

G

V

L

I

I

W

W

Y

M

D

N

G

G

A

A

L

F

V

V

P

P

W

Q

R

E

D

E

A

A

N

K

L

T

H

S

V

V

L

L

S

S

H

H

G

A

L

L

H

H

Y

Y

A

G

S

T

C

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

N

E

G

T

T

A

-

K

-

G

-

P

-

A

V

I

F

F

K

R

L

L

T

K

E

E

H

H

S

V

E

K

R
|
R

L

F

A

Y

A

N

S

S

A

A

R

K

I

V

L

L

G

R

F

M

E

E

L

I

P

P

Y

F

S

A

V

P

A

E

E

E

I

L

D

E

A

E

A

A

T

I

N

K

E

E

T

V

V

V

K

R

A

R

M

N

G

G

F

Y

T

R

D

S

A

C

Y

Y

V

I

R

R

P

P

V

L

A

A

W

W

R

M

G

G

S

A

E

K

M

A

M

L

G

G

V

V

A

N

A

P

Q

L

A

P

S

N

R

N

-

P

I

A

H

E

V

V

A

M

I

V

A

A

V

A

W

W

Q

E

W

W

P

V

S

R

Y

-

F

-

S

-

P

-

E

-

A

-

K

-

M

-

A

K

G

G

I

A

K

R

L

L

T

I

W

T

S

S

R

S

W

W

R

A

R

R

P

F

A

P

P

A

D

N

M

V

A

M

P

P

T

G

A

K

S

A

K
|
K

A

V

A

G

G

G

L

N

Y
|
Y

M

V

I

N

C

S

T

A

L

L

S

A

K

K

H

M

E

E

A

A

E

V

A

A

E

A

G

G

Y

A

Q

D

D

E

A

A

L

L

M

L

L

L

D

D

Y

E

R

E

G

G

Y

Y

L

V

A

A

E
|
E

A

G

T

S

G
|
G

A

E

N

N

L

L

F

F

L

F

V

V

M

R

D

D

G

G

K

V

I

I

H

Y

T

A

P

L

K

E

P

H

D

S

C

V

F

N

L
|
L

D

E

G
|
G

I
|
I

T
|
T

R

R

R

D

T

S

V

V

I

I

D

R

L

I

A

A

K

K

A

D

R

L

G

G

I

Y

E

E

V

V

I

Q

E

V

R

V

H

R

I

A

Q

T

P

R

D

D

E

Q

L

L

A

Y

N

M

T

A

Q

D

E

E

V

V

F

F

L

M

T

T

G

G

T
|
T

A

A

E

E

V

V

T

T

P

P

V

V

G

S

Q

M

I

I

active site: F35 (= F41), G37 (= G43), K150 (= K159), E184 (= E193), L207 (= L216)
binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), T247 (= T256)

2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
41% identity, 89% coverage: 9:266/290 of query aligns to 3:254/294 of 2ej2A

query
sites
2ej2A

R

K

D

A

G

G

V

L

I

I

W

W

Y

M

D

N

G

G

A

A

L

F

V

V

P

P

W

Q

R

E

D

E

A

A

N

K

L

T

H

S

V

V

L

L

S

S

H

H

G

A

L

L

H

H

Y

Y

A

G

S

T

C

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

N

E

G

T

T

A

-

K

-

G

-

P

-

A

V

I

F

F

K

R

L

L

T

K

E

E

H

H

S

V

E

K

R
|
R

L

F

A

Y

A

N

S

S

A

A

R

K

I

V

L

L

G

R

F

M

E

E

L

I

P

P

Y

F

S

A

V

P

A

E

E

E

I

L

D

E

A

E

A

A

T

I

N

K

E

E

T

V

V

V

K

R

A

R

M

N

G

G

F

Y

T

R

D

S

A

C

Y
|
Y

V

I

R

R

P

P

V

L

A

A

W

W

R

M

G

G

S

A

E

K

M

A

M

L

G

G

V

V

A

N

A

P

Q

L

A

P

S

N

R

N

-

P

I

A

H

E

V

V

A

M

I

V

A

A

V

A

W

W

Q

E

W

W

P

-

S

-

Y

-

F

-

S

-

P

-

E

-

A

-

K

-

M

-

A

-

G

G

I

A

K

R

L

L

T

I

W

T

S

S

R

S

W

W

R

A

R

R

P

F

A

P

P

A

D

N

M

V

A

M

P

P

T

G

A

K

S

A

K
|
K

A

V

A

G

G

G

L

N

Y
|
Y

M

V

I

N

C

S

T

A

L

L

S

A

K

K

H

M

E

E

A

A

E

V

A

A

E

A

G

G

Y

A

Q

D

D

E

A

A

L

L

M

L

L

L

D

D

Y

E

R

E

G

G

Y

Y

L

V

A

A

E
|
E

A

G

T

S

G
|
G

A
x
E

N

N

L

L

F

F

L

F

V

V

M

R

D

D

G

G

K

V

I

I

H

Y

T

A

P

L

K

E

P

H

D

S

C

V

F

N

L
|
L

D

E

G
|
G

I
|
I

T
|
T

R

R

R

D

T

S

V

V

I

I

D

R

L

I

A

A

K

K

A

D

R

L

G

G

I

Y

E

E

V

V

I

Q

E

V

R

V

H

R

I

A

Q

T

P

R

D

D

E

Q

L

L

A

Y

N

M

T

A

Q

D

E

E

V

V

F

F

L

M

T

T

G

G

T
|
T

A
|
A

A

A

E

E

V

V

T

T

P

P

V

V

G

S

Q

M

I

I

active site: F35 (= F41), G37 (= G43), K147 (= K159), E181 (= E193), L204 (= L216)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R60), Y94 (= Y96), Y152 (= Y164), E181 (= E193), G184 (= G196), E185 (≠ A197), L204 (= L216), G206 (= G218), I207 (= I219), T208 (= T220), T244 (= T256), A245 (= A257)

6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
38% identity, 96% coverage: 13:290/290 of query aligns to 9:288/301 of 6thqB

query
sites
6thqB

I

V

W

W

Y

L

D

D

G

G

A

R

L

L

V

V

P

D

W

E

R

E

D

E

A

A

N

K

L

V

H

T

V

V

L

L

S

S

H

P

G

S

L

L

H

N

Y

Y

A

G

S

F

C

G

V

V

F
|
F

E

E

G

G

E

I

R

R

V

A

Y

Y

-

W

-

N

-

G

-

E

N

N

G

L

T

Y

V

V

F

F

K

R

L

L

T

R

E

D

H

H

S

M

E

E

R
|
R

L

L

A

L

A

R

S

S

A

A

R

K

I

I

L

I

G

G

F

L

E

D

L

V

P

P

Y

Y

S

T

V

A

A

E

E

E

I

L

D

S

A

K

A

A

T

V

N

V

E

E

T

T

V

V

K

R

A

A

M

N

G

G

F

F

T

K

-

E

D

D

A

L

Y
|
Y

V

I

R

R

P

P

V

V

A

A

W

Y

R

I

G

S

S

K

E

P

M

Q

M

I

G

S

V

L

A

D

A

V

Q

R

A

G

S

L

R

Q

I

A

H

S

V

V

A

A

I

I

A

A

V

A

W

I

Q

P

W

F

P

G

S

K

Y

Y

F

L

S

-

P

-

E

-

A

-

K

K

M

V

A

E

G

G

I

V

K

R

L

A

T

A

W

V

S

V

R

S

W

W

R

R

P

V

A

H

P

T

D

S

M

M

A

M

P

P

T

V

A

M

S

A

K
|
K

A

A

A

T

G

G

L

I

Y
|
Y

M

L

I

N

C

S

T

I

L

M

S

A

K

A

H

V

E

E

A

A

E

R

A

A

E

R

G

G

Y

Y

Q

D

D

E

A

A

L

I

M

M

L

L

D

N

Y

A

R

E

G

G

Y

K

L

V

A

V

E
|
E

A

G

T

S

G
|
G

A
x
E

N
|
N

L

I

F

F

L

I

V

V

M

R

D

R

G

G

K

V

I

L

H

M

T

T

P

P

K

P

-

L

P

E

D

D

C

G

F

I

L
|
L

D

E

G
|
G

I
|
I

T
|
T

R

R

R

E

T

T

V

V

I

I

D

S

L

I

A

A

K

G

A

D

R

L

G

G

I

I

E

P

V

L

I

L

E

E

R

K

H

S

I

I

Q

T

P

R

D

E

E

E

L

L

A

Y

N

A

T

A

Q

D

E

E

V

A

F

F

L

F

T

V

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

V

I

G

I

Q

E

I

I

G

D

D

G

H

R

R

V

F

L

T

Q

P

R

G

G

R

P

V

I

C

T

E

Q

T

K

L

I

V

A

K

E

D

T

Y

Y

D

R

A

R

L

I

V

V

R

L

G

G

active site: F37 (= F41), K156 (= K159), E190 (= E193), L214 (= L216)
binding pyridoxal-5'-phosphate: R60 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), N195 (= N198), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), T254 (= T256)
binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R60), Y97 (= Y96), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (≠ A197), N195 (= N198), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256), A255 (= A257)

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
36% identity, 94% coverage: 12:284/290 of query aligns to 4:278/290 of 5e25A

query
sites
5e25A

V

L

I

V

W

Y

Y

M

D

N

G

G

A

E

L

F

V

V

P

P

W

E

R

S

D

Q

A

A

N

K

L

V

H

S

V

V

L

F

S

D

H

H

G

G

L

F

H

L

Y

Y

A

G

S

D

C

G

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

N

N

G

G

T

K

V

V

F

F

K

K

L

L

T

Y

E

E

H

H

S

I

E

D

R
|
R

L

L

A

Y

A

D

S

C

A

A

R

R

I

V

L

I

G

D

F

L

E

K

L

I

P

P

Y

L

S

S

V

K

A

E

E

E

I

F

D

A

A

E

A

A

T

I

N

L

E

E

T

T

V

L

K

R

A

R

M

N

G

N

F

L

T

R

D

D

A

A

Y
|
Y

V

I

R

R

P

P

V

I

A

V

W

T

R

R

G

G

S

A

E

G

M

D

M

L

G

G

V

L

-

D

A

P

A

R

Q

K

A

C

S

P

R

S

I

P

H

N

V

V

A

I

I

I

A

I

V

T

W

K

Q

P

W

W

P

G

S

K

Y

L

F

Y

S

G

P

-

E

D

A

L

K

Y

M

E

A

K

G

G

I

L

K

K

L

A

T

I

W

T

S

V

R

A

W

I

R

R

P

N

A

A

P

I

D

D

M

S

A

L

P

P

T

P

A

N

S

I

K
|
K

A

S

A

L

G

N

L

-

Y
|
Y

M

L

I

N

C

N

T

I

L

L

S

A

K

K

H

I

E

E

A

A

E

N

A

A

E

K

G

G

Y

G

Q

D

D

E

A

A

L

I

M

F

L

L

D

D

Y

H

R

N

G

G

Y

Y

L

I

A

S

E
|
E

A

G

T

S

G
|
G

A
x
D

N

N

L

I

F

F

L

I

V

V

M

K

D

N

G

G

K

T

I

I

H

T

T

T

P

P

K

P

P

T

D

L

C

N

F

N

L
|
L

D

K

G
|
G

I
|
I

T
|
T

R

R

R

Q

T

V

V

V

I

I

D

E

L

L

A

I

K

N

A

E

R

L

G

E

I

I

E

P

V

F

I

R

E

E

R

A

H

N

I

I

Q

G

P

L

D

F

E

D

L

L

A

Y

N

S

T

A

Q

D

E

E

V

I

F

F

L

V

T

T

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

A

P

P

V

V

G

T

Q

Y

I

I

G

-

D

D

H

G

R

R

F

T

T

V

-

G

-

N

-

G

-

K

P

P

G

G

R

K

V

V

C

T

E

K

T

M

L

L

V

M

K

E

D

K

Y

F

active site: F33 (= F41), G35 (= G43), K151 (= K159), E184 (= E193), L207 (= L216)
binding 2-oxoglutaric acid: Y88 (= Y96), K151 (= K159), T247 (= T256), A248 (= A257)
binding pyridoxal-5'-phosphate: R52 (= R60), K151 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), D188 (≠ A197), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), G246 (= G255), T247 (= T256)

5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
34% identity, 95% coverage: 13:287/290 of query aligns to 4:280/290 of 5mr0D

query
sites
5mr0D

I

V

W

Y

Y

M

D

D

G

G

A

E

L

F

V

V

P

P

W

E

R

N

D

E

A

A

N

K

L

V

H

S

V

I

L

F

S

D

H

H

G

G

L

F

H

L

Y

Y

A

G

S

D

C

G

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

N

N

G

G

T

R

V

V

F

F

K

R

L

L

T

K

E

E

H

H

S

I

E

D

R
|
R

L

L

A

Y

A

D

S

S

A

A

R

K

I

A

L

I

G

D

F

L

E

E

L

I

P

P

Y

I

S

T

V

K

A

E

E

E

I

F

D

M

A

E

A

I

T

I

N

L

E

E

T

T

V

L

K

R

A

K

M

N

G

N

F

L

T

R

D

D

A

A

Y

Y

V

I

R

R

P

P

V

I

A

V

W

T

R

R

G

G

S

I

E

G

M

D

M

L

G
|
G

V
x
L

A

D

A

P

Q

R

A

K

S

C

R

Q

I

N

H

P

V

S

A

I

I

I

A

V

V

I

W

T

Q

K

-

P

W

W

P

G

S

K

Y

L

F

Y

S

G

P

-

E

D

A

L

K

Y

M

E

A

K

G

G

I

L

K

T

L

A

T

I

W

T

S

V

R

A

W

V

R

R

P

N

A

S

P

F

D

D

M

A

A

L

P

P

T

P

A

N

S

I

K
|
K

A

S

A

L

G

N

L

-

Y
|
Y

M

L

I

N

C

N

T

I

L

L

S

A

K

K

H

I

E

E

A

A

E

N

A

A

E

K

G

G

Y

G

Q

D

D

E

A

A

L

I

M

F

L

L

D

D

Y

R

R

N

G

G

Y

Y

L

V

A

S

E
|
E

A

G

T

S

G
|
G

A
x
D

N

N

L

I

F

F

L

V

V

V

M

K

D

N

G

G

K

A

I

I

H

T

T

T

P

P

K

P

P

T

D

I

C

N

F

N

L
|
L

D

R

G

G

I
|
I

T
|
T

R

R

R

E

T

A

V

V

I

I

D

E

L

I

A

I

K

N

A

R

R

L

G

G

I

I

E

P

V

F

I

K

E

E

R

T

H

N

I

I

Q

G

P

L

D

Y

E

D

L

L

A

Y

N

T

T

A

Q

D

E

E

V

V

F

F

L

V

T

T

G
|
G

T
|
T

A

A

E

E

V

I

T

A

P

P

V

I

-

V

-

I

-

D

-

G

G

R

Q

K

I

I

G

G

D

D

H

G

R

K

F

-

T

-

P

P

G

G

R

E

V

I

C

T

E

R

T

K

L

L

V

M

K

E

D

E

Y

F

D

S

A

K

L

L

active site: F32 (= F41), G34 (= G43), K150 (= K159), E183 (= E193), L206 (= L216)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R60), G100 (= G109), L101 (≠ V110), K150 (= K159), Y154 (= Y164), E183 (= E193), G186 (= G196), D187 (≠ A197), L206 (= L216), I209 (= I219), T210 (= T220), G245 (= G255), T246 (= T256)

6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
31% identity, 96% coverage: 13:290/290 of query aligns to 6:292/307 of 6q8eA

query
sites
6q8eA

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active site: F34 (= F41), K156 (= K159), E190 (= E193), L214 (= L216)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R59 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), S194 (≠ A197), C195 (≠ N198), L214 (= L216), S216 (≠ G218), I217 (= I219), T218 (= T220), G254 (= G255), T255 (= T256)

6h65C Crystal structure of the branched-chain-amino-acid aminotransferase from haliangium ochraceum
31% identity, 90% coverage: 14:273/290 of query aligns to 8:279/308 of 6h65C

query
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active site: F35 (= F41), K158 (= K159), E192 (= E193), L216 (= L216)
binding pyridoxal-5'-phosphate: R60 (= R60), K158 (= K159), Y163 (= Y164), E192 (= E193), A196 (= A197), L216 (= L216), S218 (≠ G218), V219 (≠ I219), T220 (= T220), G256 (= G255), T257 (= T256)

7neaA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum (m3 mutant). (see paper)
30% identity, 96% coverage: 13:290/290 of query aligns to 6:292/309 of 7neaA

query
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7neaA

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