SitesBLAST

Comparing AZOBR_RS16935 FitnessBrowser__azobra:AZOBR_RS16935 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Found 20 (the maximum) hits to proteins with known functional sites

2e9fB Crystal structure of t.Th.Hb8 argininosuccinate lyase complexed with l-arginine
51% identity, 93% coverage: 34:477/480 of query aligns to 1:445/450 of 2e9fB

• active site: E71 (= E104), T146 (= T177), H147 (= H178), S268 (= S299), S269 (= S300), K274 (= K305), E281 (= E312)
• binding arginine: D72 (= D105), R98 (= R131), N99 (= N132), V102 (= V135), Y308 (= Y339), Q313 (= Q344), K316 (= K347)

1tj7B Structure determination and refinement at 2.44 a resolution of argininosuccinate lyase from e. Coli (see paper)
48% identity, 94% coverage: 30:479/480 of query aligns to 1:450/451 of 1tj7B

• active site: H150 (= H178), S271 (= S299), K277 (= K305), E284 (= E312)
• binding phosphate ion: S271 (= S299), M274 (= M302), K277 (= K305), N279 (= N307)

P04424 Argininosuccinate lyase; ASAL; Arginosuccinase; EC 4.3.2.1 from Homo sapiens (Human) (see 12 papers)
45% identity, 93% coverage: 20:464/480 of query aligns to 2:444/464 of P04424

• R12 (= R30) to Q: in ARGINSA; 18-fold reduction in catalytic efficiency toward argininosuccinate; dbSNP:rs145138923
• D31 (= D49) to N: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs754995756
• K51 (≠ R69) mutation to N: 2-fold reduction in activity.
• K69 (≠ R87) modified: N6-acetyllysine
• E73 (= E91) to K: in ARGINSA; complete loss of argininosuccinate lyase activity; abolishes protein expression
• D87 (= D105) to G: in ARGINSA; loss of argininosuccinate lyase activity; dbSNP:rs752100894
• H89 (= H107) mutation to Q: 10-fold reduction in activity.
• R94 (≠ A112) to C: in ARGINSA; severe; dbSNP:rs374304304
• R95 (= R113) to C: in ARGINSA; loss of argininosuccinate lyase activity; dbSNP:rs28940585
• R113 (= R131) to Q: in ARGINSA; complete loss of argininosuccinate lyase activity; no effect on protein expression; no effect on nitric oxide production; dbSNP:rs752783461
• D120 (= D138) to E: in ARGINSA; severe
• V178 (≠ E196) to M: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs28941473
• T181 (≠ G199) to S: in a breast cancer sample; somatic mutation
• R182 (= R200) to Q: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression; dbSNP:rs751590073
• R186 (= R204) to Q: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression; dbSNP:rs752397242
• G200 (= G218) to V: in a breast cancer sample; somatic mutation
• R236 (= R254) to W: in ARGINSA; complete loss of argininosuccinate lyase activity; no effect on protein expression; no effect on NOS complex formation; dbSNP:rs761268464
• D237 (= D255) to N: in ARGINSA; severe; dbSNP:rs552951774
• Q286 (= Q304) to R: in ARGINSA; complete loss of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs28941472
• K288 (= K306) modified: N6-acetyllysine; mutation to R: Refractory to inhibition by TSA and NAM and by addition of extra amino acids. No effect on protein structure.
• R297 (= R315) to Q: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs750431938
• R306 (≠ S324) to W: in ARGINSA; severe; dbSNP:rs868834862
• Q326 (= Q344) to L: in ARGINSA; severe
• V335 (≠ A353) to L: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression
• M360 (≠ L378) to T: in ARGINSA; loss of argininosuccinate lyase activity; may cause protein misfolding; dbSNP:rs875989948
• M382 (≠ I402) to R: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression
• R385 (= R405) to L: in ARGINSA; severe
• H388 (= H408) to Q: in ARGINSA; severe
• A398 (= A418) to D: in ARGINSA; impairs tetramer formation likely due to protein misfolding; loss of argininosuccinate lyase activity

Sites not aligning to the query:

• 456 R → W: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression; dbSNP:rs759396688

P24058 Argininosuccinate lyase; ASAL; Arginosuccinase; Delta crystallin II; Delta-2 crystallin; EC 4.3.2.1 from Anas platyrhynchos (Mallard) (Anas boschas) (see 4 papers)
43% identity, 94% coverage: 25:477/480 of query aligns to 9:459/468 of P24058

• W11 (= W27) mutation to A: 98% decrease in catalytic efficiency.; mutation to F: 90% decrease in catalytic efficiency.; mutation to M: 99% decrease in catalytic efficiency.; mutation to R: 97% decrease in catalytic efficiency.; mutation to Y: 50% decrease in catalytic efficiency.
• S29 (= S45) binding in chain A; mutation to A: 10% decrease in catalytic efficiency.
• D33 (= D49) mutation to N: 99% decrease in catalytic efficiency.
• D89 (= D105) mutation to N: Loss of activity.
• N116 (= N132) binding in chain A; mutation to D: 99% decrease in catalytic efficiency.
• D117 (= D133) mutation to A: 55% decrease in catalytic efficiency.; mutation to E: 58% decrease in catalytic efficiency.
• T161 (= T177) binding in chain C; mutation to A: Loss of activity.; mutation to D: Loss of activity.; mutation to S: 30% decrease in catalytic efficiency.; mutation to V: Loss of activity.
• H162 (= H178) mutation to E: Loss of activity.
• R238 (= R254) mutation to Q: Loss of activity.
• T281 (= T297) mutation to V: 80% decrease in catalytic efficiency.
• S283 (= S299) mutation to A: Loss of activity.; mutation to C: Loss of activity.; mutation to D: Loss of activity.; mutation to H: Loss of activity.; mutation to T: Loss of activity.
• N291 (= N307) binding in chain B; mutation to L: Loss of activity.
• D293 (= D309) mutation to N: 99% decrease in catalytic efficiency.
• E296 (= E312) mutation to D: Loss of activity.
• Y323 (= Y339) binding in chain A
• K325 (= K341) mutation to N: 99% decrease in catalytic efficiency.
• Q328 (= Q344) binding in chain A
• D330 (= D346) mutation to N: Loss of activity.
• K331 (= K347) binding in chain A; mutation to Q: Loss of activity.

1k7wD Crystal structure of s283a duck delta 2 crystallin mutant (see paper)
43% identity, 92% coverage: 38:477/480 of query aligns to 5:442/450 of 1k7wD

• active site: E71 (= E104), T144 (= T177), H145 (= H178), A266 (≠ S299), S267 (= S300), K272 (= K305), E279 (= E312)
• binding argininosuccinate: S12 (= S45), R98 (= R131), N99 (= N132), V102 (= V135), T144 (= T177), H145 (= H178), Y306 (= Y339), Q311 (= Q344), K314 (= K347)

1hy0A Crystal structure of wild type duck delta 1 crystallin (eye lens protein) (see paper)
41% identity, 92% coverage: 38:477/480 of query aligns to 3:440/447 of 1hy0A

• active site: E69 (= E104), T142 (= T177), H143 (= H178), S264 (= S299), S265 (= S300), K270 (= K305), E277 (= E312)
• binding sulfate ion: S264 (= S299), M267 (= M302), K270 (= K305), N272 (= N307)

P02521 Delta-1 crystallin; Delta crystallin I from Gallus gallus (Chicken) (see paper)
39% identity, 95% coverage: 20:477/480 of query aligns to 2:457/466 of P02521

• A2 (= A20) modified: Blocked amino end (Ala)

1dcnB Inactive mutant h162n of delta 2 crystallin with bound argininosuccinate (see paper)
39% identity, 92% coverage: 38:477/480 of query aligns to 3:412/418 of 1dcnB

• active site: N130 (≠ H178), E249 (= E312)
• binding argininosuccinate: T129 (= T177), A134 (= A182)

6ienB Substrate/product bound argininosuccinate lyase from mycobacterium tuberculosis (see paper)
44% identity, 93% coverage: 35:479/480 of query aligns to 1:448/454 of 6ienB

• binding argininosuccinate: H73 (= H107), S97 (= S130), R98 (= R131), N99 (= N132), V102 (= V135), T144 (= T177), H145 (= H178), S266 (= S299), S267 (= S300), M269 (= M302), K272 (= K305), N274 (= N307), Y306 (= Y339), Q311 (= Q344), K314 (= K347)

6ienA Substrate/product bound argininosuccinate lyase from mycobacterium tuberculosis (see paper)
43% identity, 93% coverage: 35:479/480 of query aligns to 1:446/452 of 6ienA

• binding argininosuccinate: S97 (= S130), R98 (= R131), N99 (= N132), V102 (= V135), T144 (= T177), H145 (= H178), Y304 (= Y339), Q309 (= Q344), K312 (= K347)
• binding fumaric acid: S266 (= S299), S267 (= S300), K270 (= K305), N272 (= N307)

6ienC Substrate/product bound argininosuccinate lyase from mycobacterium tuberculosis (see paper)
40% identity, 93% coverage: 35:479/480 of query aligns to 1:412/418 of 6ienC