SitesBLAST
Comparing AZOBR_RS19235 AZOBR_RS19235 actetate permease to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 12 hits to proteins with known functional sites (download)
P07117 Sodium/proline symporter; Proline carrier; Proline permease; Propionate transporter from Escherichia coli (strain K12) (see 4 papers)
25% identity, 84% coverage: 37:501/556 of query aligns to 3:461/502 of P07117
- R257 (= R290) mutation to C: Sodium-independent binding affinity for proline.
- C281 (≠ T318) mutation to S: Does not affect proline uptake activity. Confers resistance to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
- C344 (≠ G380) mutation to S: Small decrease in proline uptake activity. Confers resistance to N-ethylmaleimide. Exhibits low Na(+)-dependent proline binding.
- C349 (≠ G385) mutation to S: Does not affect proline uptake activity. Sensitive to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
- R376 (= R413) mutation R->E,Q: No change in activity.; mutation to K: Loss of activity.
Q92911 Sodium/iodide cotransporter; Na(+)/I(-) cotransporter; Natrium iodide transporter; Sodium-iodide symporter; Na(+)/I(-) symporter; Solute carrier family 5 member 5 from Homo sapiens (Human) (see 3 papers)
24% identity, 41% coverage: 33:261/556 of query aligns to 8:237/643 of Q92911
- A102 (≠ L124) natural variant: A -> P
- H226 (≠ A250) mutation H->A,D,E,K: Significant loss of iodide transport activity but no effect on its localization to the cell membrane.
- D237 (≠ S261) mutation to A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization.
Sites not aligning to the query:
- 242 Required for homodimerization; Y→A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471. Loss of iodide transport activity; when associated with F-535.
- 243 Required for homodimerization; T→A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471.
- 471 Required for homodimerization; Q→A: No effect on localization to the cell membrane, iodide transport activity and homodimerization. Significant loss of homodimerization; when associated with A-242 or A243.
- 525 A→F: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Loss of iodide transport activity; when associated with A-242.
- 536 T → Q: requires 2 nucleotide substitutions
- 556 S → Q: requires 2 nucleotide substitutions
P31639 Sodium/glucose cotransporter 2; Na(+)/glucose cotransporter 2; Low affinity sodium-glucose cotransporter; Solute carrier family 5 member 2 from Homo sapiens (Human) (see paper)
26% identity, 39% coverage: 20:237/556 of query aligns to 7:224/672 of P31639
- V95 (vs. gap) mutation to A: Strong reduction in D-glucose transporter activity. Confers partial resistance to empagliflozin inhibition.
- F98 (= F107) mutation to A: Slightly decreases D-glucose transporter activity. Abolishes the binding to inhibitor, empagliflozin.
- V157 (vs. gap) mutation to A: Decreases D-glucose transporter activity.
Sites not aligning to the query:
- 283 L→M: Strong reduction in D-glucose transporter activity. Confers partial resistance to empagliflozin inhibition.
- 453 F→A: Slightly decreases D-glucose transporter activity. Greatly reduces the binding to inhibitor, empagliflozin.
8hezA Structure of human sglt2-map17 complex with dapagliflozin (see paper)
26% identity, 37% coverage: 35:237/556 of query aligns to 2:204/582 of 8hezA
- binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[(4-ethoxyphenyl)methyl]phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: N55 (≠ Y89), G59 (≠ A93), H60 (≠ A94), G63 (= G97), L64 (= L98), T67 (≠ M101), F78 (= F107), E79 (≠ D108)
- binding sodium ion: A53 (≠ G87), I56 (≠ M90), G57 (≠ S91)
Sites not aligning to the query:
- binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[(4-ethoxyphenyl)methyl]phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: 266, 267, 271, 301, 433, 437
- binding sodium ion: 369, 372, 373
- binding : 568, 571, 572, 575, 576, 579, 580
8hdhA Structure of human sglt2-map17 complex with canagliflozin (see paper)
26% identity, 37% coverage: 35:237/556 of query aligns to 2:204/586 of 8hdhA
- binding (2~{S},3~{R},4~{R},5~{S},6~{R})-2-[3-[[5-(4-fluorophenyl)thiophen-2-yl]methyl]-4-methyl-phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: N55 (≠ Y89), G59 (≠ A93), H60 (≠ A94), G63 (= G97), L64 (= L98), F78 (= F107), E79 (≠ D108)
- binding sodium ion: A53 (≠ G87), S54 (≠ D88), I56 (≠ M90), G57 (≠ S91)
Sites not aligning to the query:
- binding (2~{S},3~{R},4~{R},5~{S},6~{R})-2-[3-[[5-(4-fluorophenyl)thiophen-2-yl]methyl]-4-methyl-phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: 267, 271, 433, 434, 437, 506
- binding sodium ion: 369, 372, 373
- binding : 575, 579, 580, 583, 584
8hb0A Structure of human sglt2-map17 complex with ta1887 (see paper)
26% identity, 37% coverage: 35:237/556 of query aligns to 2:204/586 of 8hb0A
- binding (2R,3R,4S,5S,6R)-2-[3-[(4-cyclopropylphenyl)methyl]-4-fluoranyl-indol-1-yl]-6-(hydroxymethyl)oxane-3,4,5-triol: N55 (≠ Y89), H60 (≠ A94), G63 (= G97), L64 (= L98), T67 (≠ M101), V75 (vs. gap), F78 (= F107), E79 (≠ D108), V137 (vs. gap)
- binding sodium ion: A53 (≠ G87), I56 (≠ M90), G57 (≠ S91)
Sites not aligning to the query:
- binding (2R,3R,4S,5S,6R)-2-[3-[(4-cyclopropylphenyl)methyl]-4-fluoranyl-indol-1-yl]-6-(hydroxymethyl)oxane-3,4,5-triol: 266, 267, 271, 433, 437
- binding sodium ion: 369, 372, 373
- binding : 575, 576, 579, 580, 583, 584
8hg7A Structure of human sglt2-map17 complex with sotagliflozin (see paper)
26% identity, 37% coverage: 35:237/556 of query aligns to 2:204/590 of 8hg7A
- binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[(4-ethoxyphenyl)methyl]phenyl]-6-methylsulfanyl-oxane-3,4,5-triol: N55 (≠ Y89), G59 (≠ A93), H60 (≠ A94), G63 (= G97), L64 (= L98), E79 (≠ D108)
- binding sodium ion: A53 (≠ G87), S54 (≠ D88), I56 (≠ M90), G57 (≠ S91)
Sites not aligning to the query:
- binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[(4-ethoxyphenyl)methyl]phenyl]-6-methylsulfanyl-oxane-3,4,5-triol: 266, 267, 270, 271, 301, 433, 437
- binding sodium ion: 369, 372, 373
- binding : 579, 583, 584, 587, 588
7vsiA Structure of human sglt2-map17 complex bound with empagliflozin (see paper)
26% identity, 37% coverage: 35:237/556 of query aligns to 2:204/586 of 7vsiA
- binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[[4-[(3S)-oxolan-3-yl]oxyphenyl]methyl]phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: N55 (≠ Y89), H60 (≠ A94), G63 (= G97), L64 (= L98), V75 (vs. gap), F78 (= F107), E79 (≠ D108)
Sites not aligning to the query:
- binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[[4-[(3S)-oxolan-3-yl]oxyphenyl]methyl]phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: 266, 267, 270, 433, 434, 437
5nv9A Substrate-bound outward-open state of a na+-coupled sialic acid symporter reveals a novel na+-site (see paper)
20% identity, 52% coverage: 48:338/556 of query aligns to 13:304/480 of 5nv9A
- binding sodium ion: A52 (≠ G87), T53 (≠ D88), L55 (≠ M90), S56 (= S91), V174 (≠ T210), D178 (≠ Q214)
- binding N-acetyl-beta-neuraminic acid: T54 (≠ Y89), S56 (= S91), I58 (≠ A93), T59 (≠ A94), G77 (≠ Y112), Q78 (≠ T113), R131 (vs. gap), F239 (≠ L273)
Sites not aligning to the query:
P11170 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
20% identity, 79% coverage: 34:471/556 of query aligns to 24:488/662 of P11170
- C255 (≠ A263) modified: Disulfide link with 608
- Q457 (≠ F443) mutation to W: Drasticly decreased affinity for glucose and phlorizin.
- T460 (≠ A446) mutation to W: Decreased affinity for glucose and phlorizin.
Sites not aligning to the query:
- 608 modified: Disulfide link with 255
Q9NY91 Probable glucose sensor protein SLC5A4; Solute carrier family 5 member 4 from Homo sapiens (Human) (see paper)
22% identity, 37% coverage: 34:237/556 of query aligns to 24:227/659 of Q9NY91
Sites not aligning to the query:
- 457 E→Q: Confers sugar transport activity not found in the wild-type protein. Increased sensitivity to inhibitor phlorizin.
8hinA Structure of human sglt2-map17 complex with phlorizin (see paper)
26% identity, 37% coverage: 31:237/556 of query aligns to 5:200/588 of 8hinA
- binding 1-[2-[(2S,3R,4S,5S,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-4,6-bis(oxidanyl)phenyl]-3-(4-hydroxyphenyl)propan-1-one: S46 (≠ G75), A49 (≠ G78), S50 (≠ F79), G53 (= G82), D177 (≠ Q214), T181 (≠ A218)
Sites not aligning to the query:
- binding 1-[2-[(2S,3R,4S,5S,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-4,6-bis(oxidanyl)phenyl]-3-(4-hydroxyphenyl)propan-1-one: 276, 369
- binding : 574, 578, 581, 582, 585, 586
Query Sequence
>AZOBR_RS19235 AZOBR_RS19235 actetate permease
MSAARLLAAASAALLPVLVAGPVHAAAIDGAVERQPVNVTAIAMFLLFVAGTLGITYWAS
KRTRSASDFYTAGGGISGFQNGLAIAGDYMSAAAFLGLSGMVFAKGFDGVIYTIGFLVGW
PLMLFLIAERLRNLGRFTFADVASYRLGQTPIRSLAAVGSLTVVCFYLIAQMVGAGKLIQ
LLFGLDYTYAVVMVGVLMILYVTFGGMLATTWVQIIKAVMLLGGCTVLVGLALAQFGFNP
ERLLQQAVAAHAANAAILRPSAAMADPIAAVSLSLALMCGPAGLPHILMRFFTVPDAKEA
RKSVVYATGFIGYFFILTVTIGFLAIVIVGTNPAYLDAAGKILGGGNMAAIHLSKAIGGN
LFLGFISAVAFATILAVVAGLTLAGASAVSHDLYARVLKKGNATEASEMRVSRLATLALG
VIAITLGLLFENQNIAFMVGLAFGLAASVNFPVLILSIFWKGLTTRGAFIGGFAGLVSCV
AFVVLGPTVWVSVFKFPAPIFPYEHPALFSMVIAFATTWLFSVTDRSARAAAEAKAYEYQ
YIRSETGLGAASAASH
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory