SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AZOBR_RS19585 FitnessBrowser__azobra:AZOBR_RS19585 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5cevA Arginase from bacillus caldevelox, l-lysine complex (see paper)
41% identity, 96% coverage: 1:301/315 of query aligns to 3:298/298 of 5cevA

query
sites
5cevA

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active site: H98 (= H97), D121 (= D124), H123 (= H126), D125 (= D128), H138 (= H141), D225 (= D228), D227 (= D230), E270 (= E273)
binding guanidine: M194 (= M197), H195 (≠ R198), D198 (= D201), R248 (= R251), H251 (= H254), L252 (= L255), E255 (= E258)
binding lysine: H123 (= H126), D125 (= D128), N127 (= N130), S134 (= S137), H138 (= H141), G139 (= G142), D177 (= D180), T239 (= T242), E270 (= E273)
binding manganese (ii) ion: H98 (= H97), D121 (= D124), D121 (= D124), H123 (= H126), D125 (= D128), D225 (= D228), D225 (= D228), D227 (= D230)

4cevA Arginase from bacillus caldevelox, l-ornithine complex (see paper)
41% identity, 96% coverage: 1:301/315 of query aligns to 3:298/298 of 4cevA

query
sites
4cevA

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active site: H98 (= H97), D121 (= D124), H123 (= H126), D125 (= D128), H138 (= H141), D225 (= D228), D227 (= D230), E270 (= E273)
binding guanidine: M194 (= M197), H195 (≠ R198), D198 (= D201), R248 (= R251), H251 (= H254), L252 (= L255), E255 (= E258)
binding manganese (ii) ion: H98 (= H97), D121 (= D124), D121 (= D124), H123 (= H126), D125 (= D128), D225 (= D228), D225 (= D228), D227 (= D230)
binding L-ornithine: H123 (= H126), D125 (= D128), N127 (= N130), P133 (= P136), S134 (= S137), H138 (= H141), G139 (= G142), D177 (= D180)

3cevA Arginase from bacillus caldevelox, complexed with l-arginine (see paper)
41% identity, 96% coverage: 1:301/315 of query aligns to 3:298/298 of 3cevA

query
sites
3cevA

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active site: H98 (= H97), D121 (= D124), H123 (= H126), D125 (= D128), H138 (= H141), D225 (= D228), D227 (= D230), E270 (= E273)
binding arginine: H98 (= H97), H123 (= H126), D125 (= D128), N127 (= N130), P133 (= P136), S134 (= S137), H138 (= H141), G139 (= G142), D177 (= D180), M194 (= M197), H195 (≠ R198), D198 (= D201), D225 (= D228), T239 (= T242), R248 (= R251), H251 (= H254), L252 (= L255), E255 (= E258), E270 (= E273), S291 (= S294), E295 (≠ R298), L297 (≠ I300)
binding manganese (ii) ion: H98 (= H97), D121 (= D124), D125 (= D128), D225 (= D228)

2cevA Arginase from bacillus caldevelox, native structure at ph 8.5 (see paper)
41% identity, 96% coverage: 1:301/315 of query aligns to 3:298/298 of 2cevA

query
sites
2cevA

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P

P

A

S

I

D

A

A

P

P

A

G

V

V

G

G

T

T

A

P

V

V

L

I

G

G

G

G

A

L

T

T

Y

Y

R
|
R

E

E

A

S

H
|
H

L
|
L

I

A

M

M

E
|
E

M

M

L

L

H

A

D

E

A

A

G

Q

V

I

V

I

G

T

S

S

L

A

D

E

V

F

V

V

E
|
E

L

V

N

N

P

P

F

I

L

L

D

D

E

E

R

R

G

N

K

K

S

T

A

A

L

S

L

V

L

A

V

V

D

A

L

L

V

M

A

G

S

S

L

L

F

F

G

G

R

E

R

K

I
x
L

I

M

active site: H98 (= H97), D121 (= D124), H123 (= H126), D125 (= D128), H138 (= H141), D225 (= D228), D227 (= D230), E270 (= E273)
binding guanidine: M194 (= M197), H195 (≠ R198), D198 (= D201), R248 (= R251), H251 (= H254), L252 (= L255), E255 (= E258), L297 (≠ I300)
binding manganese (ii) ion: H98 (= H97), D121 (= D124), D121 (= D124), H123 (= H126), D125 (= D128), D225 (= D228), D225 (= D228), D227 (= D230)

1cevA Arginase from bacillus caldovelox, native structure at ph 5.6 (see paper)
41% identity, 96% coverage: 1:301/315 of query aligns to 4:299/299 of 1cevA

query
sites
1cevA

V

I

E

S

I

I

I

I

G

G

V

V

P

P

I

M

E

D

A

L

G

G

A

Q

G

T

H

R

P

R

G

G

A

V

L

D

M

M

G

G

P

P

A

S

A

A

L

M

R

R

T

Y

A

A

G

G

L

V

V

I

R

E

A

R

L

L

R

E

E

R

L

L

G

H

H

Y

E

D

V

I

R

E

D

D

L

L

G

G

D

D

L

I

G

P

R

I

G

G

D

K

L

-

T

A

P

E

A

R

P

L

W

H

S

E

E

Q

G

G

D

D

A

S

A

R

T

L

A

R

R

N

L

L

A

K

E

A

I

V

T

A

A

E

W

A

S

N

R

E

A

K

L

L

A

A

D

A

R

A

S

V

Y

D

A

Q

M

V

M

V

Q

Q

A

R

G

G

G

R

V

F

P

P

V

L

F

V

L

L

G

G

G

G

D

D

H
|
H

S

S

L

I

S

A

M

I

G

G

S

T

V

L

T

A

G

G

V

V

A

A

R

K

H

H

C

Y

R

E

E

R

T

L

G

G

K

-

P

-

L

-

F

-

V

V

L

I

W

W

L

Y

D
|
D

A

A

H
|
H

G

G

D
|
D

F

V

N

N

T

T

P

A

H

E

T

T

S

S

P

P

S

S

G

G

N

N

M

I

H
|
H

G

G

M

M

P

P

V

L

A

A

-

A

-

S

L

L

L

G

C

F

G

G

E

H

D

P

G

A

F

L

D

T

N

Q

V

I

-

G

-

G

F

Y

P

S

P

P

E

K

Q

-

R

-

A

-

T

-

V

I

N

K

P

P

A

E

H

H

V

V

H

V

L

L

F

I

G

G

I

V

R

R

S

S

L

L

D

D

P

E

G

G

E

E

R

K

R

K

L

F

L

I

R

R

A

E

R

K

G

G

V

I

D

K

V

I

V

Y

D

T

M

M

R

H

L

E

I

V

D

D

E

R

H

L

G

G

V

M

G

T

V

R

H

V

M

M

R

E

R

E

I

T

I

I

E

A

R

Y

V

L

R

K

Q

E

A

R

G

T

G

D

H

G

L

V

H

H

V

L

S

S

L

L

D
|
D

V

L

D
|
D

F

G

L

L

D

D

P

P

A

S

I

D

A

A

P

P

A

G

V

V

G

G

T

T

A

P

V

V

L

I

G

G

G

G

A

L

T

T

Y

Y

R

R

E

E

A

S

H

H

L

L

I

A

M

M

E

E

M

M

L

L

H

A

D

E

A

A

G

Q

V

I

V

I

G

T

S

S

L

A

D

E

V

F

V

V

E
|
E

L

V

N

N

P

P

F

I

L

L

D

D

E

E

R

R

G

N

K

K

S

T

A

A

L

S

L

V

L

A

V

V

D

A

L

L

V

M

A

G

S

S

L

L

F

F

G

G

R

E

R

K

I

L

I

M

active site: H99 (= H97), D122 (= D124), H124 (= H126), D126 (= D128), H139 (= H141), D226 (= D228), D228 (= D230), E271 (= E273)
binding manganese (ii) ion: H99 (= H97), D122 (= D124), D122 (= D124), H124 (= H126), D126 (= D128), D226 (= D228), D226 (= D228), D228 (= D230)

P53608 Arginase; EC 3.5.3.1 from Bacillus caldovelox (see paper)
41% identity, 96% coverage: 1:301/315 of query aligns to 4:299/299 of P53608

query
sites
P53608

V

I

E

S

I

I

I

I

G

G

V

V

P

P

I

M

E

D

A

L

G

G

A

Q

G

T

H

R

P

R

G

G

A

V

L

D

M

M

G

G

P

P

A

S

A

A

L

M

R

R

T

Y

A

A

G

G

L

V

V

I

R

E

A

R

L

L

R

E

E

R

L

L

G

H

H

Y

E

D

V

I

R

E

D

D

L

L

G

G

D

D

L

I

G

P

R

I

G

G

D

K

L

-

T

A

P

E

A

R

P

L

W

H

S

E

E

Q

G

G

D

D

A

S

A

R

T

L

A

R

R

N

L

L

A

K

E

A

I

V

T

A

A

E

W

A

S

N

R

E

A

K

L

L

A

A

D

A

R

A

S

V

Y

D

A

Q

M

V

M

V

Q

Q

A

R

G

G

G

R

V

F

P

P

V

L

F

V

L

L

G

G

G

G

D

D

H
|
H

S

S

L

I

S

A

M

I

G

G

S

T

V

L

T

A

G

G

V

V

A

A

R

K

H

H

C

Y

R

E

E

R

T

L

G

G

K

-

P

-

L

-

F

-

V

V

L

I

W

W

L

Y

D
|
D

A

A

H
|
H

G
|
G

D
|
D

F
x
V

N
|
N

T

T

P

A

H

E

T

T

S

S

P

P

S
|
S

G
|
G

N
|
N

M

I

H

H

G

G

M

M

P

P

V

L

A

A

-

A

-

S

L

L

L

G

C

F

G

G

E

H

D

P

G

A

F

L

D

T

N

Q

V

I

-

G

-

G

F

Y

P

S

P

P

E

K

Q

-

R

-

A

-

T

-

V

I

N

K

P

P

A

E

H

H

V

V

H

V

L

L

F

I

G

G

I

V

R

R

S

S

L

L

D
|
D

P

E

G

G

E

E

R

K

R

K

L

F

L

I

R

R

A

E

R

K

G

G

V

I

D

K

V

I

V

Y

D

T

M

M

R

H

L

E

I

V

D

D

E

R

H

L

G

G

V

M

G

T

V

R

H

V

M

M

R

E

R

E

I

T

I

I

E

A

R

Y

V

L

R

K

Q

E

A

R

G

T

G

D

H

G

L

V

H

H

V

L

S

S

L

L

D
|
D

V

L

D
|
D

F

G

L

L

D

D

P

P

A

S

I

D

A

A

P

P

A

G

V

V

G

G

T
|
T

A

P

V

V

L

I

G

G

G

G

A

L

T

T

Y

Y

R

R

E

E

A

S

H

H

L

L

I

A

M

M

E

E

M

M

L

L

H

A

D

E

A

A

G

Q

V

I

V

I

G

T

S

S

L

A

D

E

V

F

V

V

E
|
E

L

V

N

N

P

P

F

I

L

L

D

D

E

E

R

R

G

N

K

K

S

T

A

A

L

S

L

V

L

A

V

V

D

A

L

L

V

M

A

G

S

S

L

L

F

F

G

G

R

E

R

K

I

L

I

M

H99 (= H97) binding
D122 (= D124) binding ; binding
H124 (= H126) binding
HGDVN 124:128 (≠ HGDFN 126:130) binding
D126 (= D128) binding
SGN 135:137 (= SGN 137:139) binding
D178 (= D180) binding
D226 (= D228) binding ; binding
D228 (= D230) binding
T240 (= T242) binding
E271 (= E273) binding

2ef5A Crystal structure of the arginase from thermus thermophilus
49% identity, 96% coverage: 1:301/315 of query aligns to 3:273/273 of 2ef5A

query
sites
2ef5A

V

V

E

A

I

V

I

V

G

G

V

V

P
|
P

I

M

E

D

A

L

G

G

A

V

G
x
D

H

-

P

-

G

-

A

-

L

-

M
|
M

G

G

P

P

A
x
S

A

A

L

L

R

R

T

Y

A

A

G

R

L

L

V

L

R

E

A

Q

L

L

R

E

E

D

L

L

G

G

H

Y

E

T

V

V

R

E

D

D

L

L

G

G

D

D

L

V

G

-

R

-

G

-

D

-

L

-

T

-

P

-

A

-

P

-

W

-

S

-

E

-

G

-

D

P

A

V

A

S

T

L

A

A

R

Y

L

L

A

E

E

E

I

I

T

R

A

A

W

A

S

A

R

L

A

V

L

L

A

K

D

E

R

R

S

-

Y

L

A

A

M

A

M

L

Q

P

A

E

G

G

G

V

V

F

P

P

V

I

F

V

L

L

G

G

G

G

D

D

H
|
H

S

S

L

L

S

S

M

M

G

G

S

S

V

V

T

A

G

G

V

A

A

A

R

R

H

-

C

-

R

-

E

-

T

-

G

G

K

R

P

R

L

V

F

G

V

V

L

V

W

W

L

V

D
|
D

A

A

H
|
H

G

A

D
|
D

F

F

N

N

T

T

P

P

H

E

T

T

S

S

P

-

S

S

G

G

N

N

M

V

H
|
H

G

G

M

M

P

P

V

L

A

A

L

V

L

L

C

S

G

G

-

L

-

G

E

H

D

P

G

R

F

L

D

T

N

E

V

V

F

F

P

-

P

-

E

-

Q

-

R

R

A

A

T

-

V

V

N

D

P

P

A

K

H

D

V

V

H

V

L

L

F

V

G

G

I

V

R

R

S

S

L

L

D

D

P

P

G

G

E

E

R

K

R

R

L

L

L

L

R

K

A

E

R

A

G

G

V

V

D

R

V

V

V

Y

D

T

M

M

R

H

L

E

I

V

D

D

E

R

H

L

G

G

V

V

G

A

V

-

H

-

M

-

R

-

R

R

I

I

I

A

E

E

R

E

V

V

R

L

Q

K

-

H

-

L

A

Q

G

G

G

L

H

P

L

L

H

H

V

V

S

S

L

L

D
|
D

V

A

D
|
D

F

V

L

L

D

D

P

P

A

T

I

L

A

A

P

P

A

G

V

V

G

G

T

T

A

P

V

V

L

P

G

G

G

G

A

L

T

T

Y

Y

R

R

E

E

A

A

H

H

L

L

I

L

M

M

E

E

M

I

L

L

H

A

D

E

A

S

G

G

V

R

V

V

G

Q

S

S

L

L

D

D

V

L

V

V

E
|
E

L

V

N

N

P

P

F

I

L

L

D

D

E

E

R

R

G

N

K

R

S

T

A

A

L

E

L

M

L

L

V

V

D

G

L

L

V

A

A

L

S

S

L

L

F

L

G

G

R

K

R

R

I

I

I

F

active site: H80 (= H97), D102 (= D124), H104 (= H126), D106 (= D128), H118 (= H141), D200 (= D228), D202 (= D230), E245 (= E273)
binding lysine: P9 (= P7), D15 (≠ G13), M16 (= M19), S19 (≠ A22)
binding manganese (ii) ion: H80 (= H97), D102 (= D124), D102 (= D124), H104 (= H126), D106 (= D128), D200 (= D228), D200 (= D228), D202 (= D230)

6nfpC 1.7 angstrom resolution crystal structure of arginase from bacillus subtilis subsp. Subtilis str. 168
40% identity, 96% coverage: 1:301/315 of query aligns to 3:290/290 of 6nfpC

query
sites
6nfpC

V

I

E

S

I

V

I

I

G

G

V

M

P

P

I

M

E
x
D

A
x
L

G
|
G

A

Q

G

A

H
x
R

P

R

G

G

A

V

L

D

M

M

G

G

P

P

A

S

A

A

L

I

R

R

T

Y

A

A

G

H

L

L

V

I

R

E

A

R

L

L

R

S

E

D

L

M

G

G

H

Y

E

T

V

V

R

E

D

D

L

L

G

G

D

D

L

I

G

-

R

-

G

-

D

-

L

-

T

-

P

-

A

-

P

P

W

I

S

N

E

R

G

E

D

D

A

E

A

E

T

L

A

K

R
x
N

L

L

A

N

E

S

I

V

T

L

A

A

W

G

S

N

R

E

A

K

L

L

A

A

D

Q

R

K

S

V

Y

N

A

K

M

V

M

I

Q

E

A

E

G

K

G

K

V

F

P

P

V

L

F

V

L

L

G

G

G

G

D

D

H
|
H

S

S

L

I

S

A

M

I

G

G

S

T

V

L

T

A

G

G

V

T

A

A

R

K

H

H

C

Y

R

D

E

N

T

L

G

G

K

-

P

-

L

-

F

-

V

V

L

I

W

W

L

Y

D
|
D

A

A

H
|
H

G

G

D
|
D

F

L

N

N

T

T

P

L

H

E

T

T

S

S

P

P

S

S

G

G

N

N

M

I

H

H

G

G

M

M

P

P

V

L

A

A

L

V

L

S

C

L

G

G

E

-

D

I

G

G

F

H

D

E

N

S

V

L

F

V

P

N

P

L

E

E

Q

G

R

Y

A

A

-

P

T

K

V

I

N

K

P

P

A

E

H

N

V

V

H

V

L

I

F

I

G

G

I

A

R

R

S

S

L

L

D

D

P

E

G

G

E

E

R

R

R

K

L

Y

L

I

R

K

A

E

R

S

G

G

V

M

D

K

V

V

V

Y

D

T

M

M

R

H

L

E

I

I

D

D

E

R

H

L

G

G

V

M

G

T

V

K

H

V

M

I

R

E

R

E

I

T

I

L

E

D

R

Y

V

L

R

S

Q

A

A

C

G

D

G

G

H

-

L

V

H

H

V

L

S

S

L

L

D
|
D

V

L

D
|
D

F

G

L

L

D

D

P

P

A

N

I

D

A

A

P

P

A

G

V

V

G

G

T

T

A

P

V

V

L

V

G

G

G

G

A

I

T

S

Y

Y

R

R

E

E

A

S

H

H

L

L

I

A

M

M

E

E

M

M

L

L

H

Y

D

D

A

A

G

G

V

I

V

I

G

T

S

S

L

A

D

E

V

F

V

V

E

E

L

V

N

N

P

P

F

I

L

L

D

D

E

H

R

K

G

N

K

K

S

T

A

G

L

K

L

T

L

A

V

V

D

E

L

L

V

V

A

E

S

S

L

L

F

L

G

G

R

K

R

K

I

L

I

L

binding glycolic acid: D11 (≠ E9), L12 (≠ A10), G13 (= G11), R16 (≠ H14), N59 (≠ R65)
binding magnesium ion: H91 (= H97), D114 (= D124), D114 (= D124), H116 (= H126), D118 (= D128), D217 (= D228), D217 (= D228), D219 (= D230)

6nfpA 1.7 angstrom resolution crystal structure of arginase from bacillus subtilis subsp. Subtilis str. 168
40% identity, 96% coverage: 1:301/315 of query aligns to 3:292/292 of 6nfpA

query
sites
6nfpA

V

I

E

S

I

V

I

I

G

G

V

M

P

P

I

M

E

D

A

L

G

G

A

Q

G

A

H

R

P

R

G

G

A

V

L

D

M

M

G

G

P

P

A

S

A

A

L

I

R

R

T

Y

A

A

G

H

L

L

V

I

R

E

A

R

L

L

R

S

E

D

L

M

G

G

H

Y

E

T

V

V

R

E

D

D

L

L

G

G

D

D

L

I

G

-

R

-

G

-

D

-

L

-

T

-

P

P

A

I

P

N

W

R

S

E

E

K

G

I

D

D

A

E

A

E

T

L

A

K

R

N

L

L

A

N

E

S

I

V

T

L

A

A

W

G

S

N

R

E

A

K

L

L

A

A

D

Q

R

K

S

V

Y

N

A

K

M

V

M

I

Q

E

A

E

G

K

G

K

V

F

P

P

V

L

F

V

L

L

G

G

G

G

D

D

H
|
H

S

S

L

I

S

A

M

I

G

G

S

T

V

L

T

A

G

G

V

T

A

A

R

K

H

H

C

Y

R

D

E

N

T

L

G

G

K

-

P

-

L

-

F

-

V

V

L

I

W

W

L

Y

D
|
D

A

A

H
|
H

G

G

D
|
D

F

L

N

N

T

T

P

L

H

E

T

T

S

S

P

P

S

S

G

G

N

N

M

I

H

H

G

G

M

M

P

P

V

L

A

A

L

V

L

S

C

L

G

G

E

-

D

I

G

G

F

H

D

E

N

S

V

L

F

V

P

N

P

L

E

E

Q

G

R

Y

A

A

-

P

T

K

V

I

N

K

P

P

A

E

H

N

V

V

H

V

L

I

F

I

G

G

I

A

R

R

S

S

L

L

D

D

P

E

G

G

E

E

R

R

R

K

L

Y

L

I

R

K

A

E

R

S

G

G

V

M

D

K

V

V

V

Y

D

T

M

M

R

H

L

E

I

I

D

D

E

R

H

L

G

G

V

M

G

T

V

K

H

V

M

I

R

E

R

E

I

T

I

L

E

D

R

Y

V

L

R

S

Q

A

A

C

G

D

G

G

H

-

L

V

H

H

V

L

S

S

L

L

D
|
D

V

L

D
|
D

F

G

L

L

D

D

P

P

A

N

I

D

A

A

P

P

A

G

V

V

G

G

T

T

A

P

V

V

L

V

G

G

G

G

A

I

T

S

Y

Y

R

R

E

E

A

S

H

H

L

L

I

A

M

M

E

E

M

M

L

L

H

Y

D

D

A

A

G

G

V

I

V

I

G

T

S

S

L

A

D

E

V

F

V

V

E

E

L

V

N

N

P

P

F

I

L

L

D

D

E

H

R

K

G

N

K

K

S

T

A

G

L

K

L

T

L

A

V

V

D

E

L

L

V

V

A

E

S

S

L

L

F

L

G

G

R

K

R

K

I

L

I

L

binding magnesium ion: H93 (= H97), D116 (= D124), D116 (= D124), H118 (= H126), D120 (= D128), D219 (= D228), D219 (= D228), D221 (= D230)

6dktA Crystal structure of arginase from bacillus subtilis
39% identity, 96% coverage: 1:301/315 of query aligns to 3:283/283 of 6dktA

query
sites
6dktA

V

I

E

S

I

V

I

I

G

G

V

M

P

P

I

M

E

D

A

L

G

G

A

Q

G

A

H

R

P

R

G

G

A

V

L

D

M

M

G

G

P

P

A

S

A

A

L

I

R

R

T

Y

A

A

G

H

L

L

V

I

R

E

A

R

L

L

R

S

E

D

L

M

G

G

H

Y

E

T

V

V

R

E

D

D

L

L

G

G

D

D

L

I

G

-

R

-

G

-

D

-

L

-

T

-

P

-

A

-

P

-

W

-

S

-

E

-

G

-

D

-

A

-

A

P

T

I

A

N

R

N

L

L

A

N

E

S

I

V

T

L

A

A

W

G

S

N

R

E

A

K

L

L

A

A

D

Q

R

K

S

V

Y

N

A

K

M

V

M

I

Q

E

A

E

G

K

G

K

V

F

P

P

V

L

F

V

L

L

G

G

G

G

D

D

H
|
H

S

S

L

I

S

A

M

I

G

G

S

T

V

L

T

A

G

G

V

T

A

A

R

K

H

H

C

Y

R

D

E

N

T

L

G

G

K

-

P

-

L

-

F

-

V

V

L

I

W
|
W

L

Y

D
|
D

A

A

H
|
H

G

G

D
|
D

F

L

N

N

T

T

P

L

H

E

T

T

S

S

P

P

S

S

G

G

N

N

M

I

H

H

G

G

M

M

P

P

V

L

A

A

L

V

L

S

C

L

G

G

E

-

D

I

G

G

F

H

D

E

N

S

V

L

F

V

P

N

P

L

E

E

Q

G

R

Y

A

A

-

P

T

K

V

I

N

K

P

P

A

E

H

N

V

V

H

V

L

I

F

I

G

G

I

A

R

R

S

S

L

L

D

D

P

E

G

G

E

E

R

R

R

K

L

Y

L

I

R

K

A

E

R

S

G

G

V

M

D

K

V

V

V

Y

D

T

M

M

R

H

L

E

I

I

D

D

E

R

H

L

G

G

V

M

G

T

V

K

H

V

M

I

R

E

R

E

I

T

I

L

E

D

R

Y

V

L

R

S

Q

A

A

C

G

D

G

G

H

-

L

V

H

H

V

L

S

S

L

L

D
|
D

V

L

D
|
D

F

G

L

L

D

D

P

P

A

N

I

D

A

A

P

P

A

G

V

V

G

G

T

T

A

P

V

V

L

V

G

G

G

G

A

I

T

S

Y

Y

R

R

E

E

A

S

H

H

L

L

I

A

M

M

E

E

M

M

L

L

H

Y

D

D

A

A

G

G

V

I

V

I

G

T

S

S

L

A

D

E

V

F

V

V

E

E

L

V

N

N

P

P

F

I

L

L

D

D

E

H

R

K

G

N

K

K

S

T

A

G

L

K

L

T

L

A

V

V

D

E

L

L

V

V

A

E

S

S

L

L

F

L

G

G

R

K

R

K

I

L

I

L

binding manganese (ii) ion: H84 (= H97), W105 (= W122), D107 (= D124), D107 (= D124), H109 (= H126), D111 (= D128), D210 (= D228), D210 (= D228), D212 (= D230)

6nbkA Crystal structure of arginase from bacillus cereus
39% identity, 95% coverage: 1:300/315 of query aligns to 3:288/289 of 6nbkA

query
sites
6nbkA

V

I

E

S

I

V

I

I

G

G

V

V

P

P

I

M

E

D

A

L

G

G

A

Q

G

M

H

R

P

R

G

G

A

V

L

D

M

M

G

G

P

P

A

S

A

A

L

I

R

R

T

Y

A

A

G

G

L

V

V

I

R

E

A

R

L

I

R

E

E

E

L

I

G

G

H

Y

E

D

V

V

R

K

D

D

L

M

G

G

D

D

L

I

G

-

R

-

G

-

D

-

L

-

T

-

P

-

A

-

P

-

W

C

S

I

E

E

G

E

D

N

A

T

A

K

T

L

A

R

R

N

L

L

A

T

E

Q

I

V

T

A

A

T

W

V

S

C

R

N

A

E

L

L

A

A

D

S

R

K

S

V

Y
x
D

A

H

M

I

M

I

Q
x
E

A

E

G

G

G

R

V

F

P

P

V

L

F

V

L

L

G

G

G

G

D

D

H
|
H

S

S

L

I

S

A

M

I

G

G

S

T

V

L

T

A

G

G

V

V

A

A

R

K

H

H

C

Y

R

K

E

N

T

L

G

G

K

-

P

-

L

-

F

-

V

V

L

I

W

W

L

Y

D
|
D

A

A

H
|
H

G

G

D
|
D

F

L

N

N

T

T

P

E

H

E

T

T

S

S

P

P

S

S

G

G

N

N

M

I

H

H

G

G

M

M

P

S

V

L

A

A

L

A

L

S

C

L

G

G

E

Y

D

G

G

H

F

S

D

S

N

L

V

V

F

D

P

L

P

Y

E

G

Q

A

R

Y

A

P

T

K

V

V

N

K

P

K

A

E

H

N

V

V

H

V

L

I

F

I

G

G

I

A

R

R

S

A

L

L

D

D

P

E

G

G

E

E

R

K

R

D

L

F

L

I

R

R

A

N

R

E

G

G

V

I

D

K

V

V

V

F

D

S

M

M

R

H

L

E

I

I

D

D

E

R

H

M

G

G

V

M

G

T

V

A

H

V

M

M

R

E

R

E

I

T

I

I

E

A

R

Y

V

L

R

S

Q

H

A

T

G

D

G

G

H

-

L

V

H

H

V

L

S

S

L

L

D
|
D

V

L

D
|
D

F

G

L

L

D

D

P

P

A

H

I

D

A

A

P

P

A

G

V

V

G

G

T

T

A

P

V

V

L

I

G

G

G

G

A

L

T

S

Y

Y

R

R

E

E

A

S

H

H

L

L

I

A

M

M

E

E

M

M

L

L

H

A

D

E

A

A

G

D

V

I

V

I

G

T

S

S

L

A

D

E

V

F

V

V

E

E

L

V

N

N

P

T

F

I

L

L

D

D

E

E

R

R

G

N

K

R

S

T

A

A

L

T

L

T

L

A

V

V

D

A

L

L

V

M

A

G

S

S

L

L

F

F

G

G

R

E

R

K

I

L

binding manganese (ii) ion: D74 (≠ Y81), E78 (≠ Q85), H90 (= H97), D113 (= D124), D113 (= D124), H115 (= H126), D117 (= D128), D216 (= D228), D216 (= D228), D218 (= D230)

P78540 Arginase-2, mitochondrial; Arginase II; Kidney-type arginase; Non-hepatic arginase; Type II arginase; EC 3.5.3.1 from Homo sapiens (Human) (see paper)
38% identity, 95% coverage: 1:298/315 of query aligns to 26:325/354 of P78540

query
sites
P78540

V

V

E

A

I

V

I

I

G

G

V

A

P

P

I

F

E

S

A

Q

G

G

A

Q

G

K

H

R

P

K

G

G

A

V

L

E

M

H

G

G

P

P

A

A

A

A

L

I

R

R

T

E

A

A

G

G

L

L

V

M

R

K

A

R

L

L

R

S

E

S

L

L

G

G

H

C

E

H

V

L

R

K

D

D

L

F

G

G

D

D

L

L

G

S

-

F

-

T

-

P

-

V

-

P

-

K

-

D

-

D

-

L

R

Y

G

N

D

N

L

L

T

I

P

V

A

N

P

P

W

R

S

S

E

V

G

G

D

-

A

L

A

A

T

N

A

Q

R

E

L

L

A

A

E

E

I

V

T

V

A

-

W

-

S

S

R

R

A

A

L

V

A

S

D

D

R

-

S

-

Y

-

A

-

M

-

M

-

Q

-

A

-

G

G

G

Y

V

S

P

C

V

V

F

T

L

L

G

G

G

G

D

D

H
|
H

S

S

L

L

S

A

M

I

G

G

S

T

V

I

T

S

G

G

V

H

A

A

R

R

H

H

C

C

R

P

E

D

T

-

G

-

K

-

P

-

L

L

F

C

V

V

L

V

W

W

L

V

D
|
D

A

A

H
|
H

G
x
A

D
|
D

F
x
I

N
|
N

T

T

P

P

H

L

T

T

S

T

P

S

S
|
S

G
|
G

N
|
N

M

L

H

H

G

G

M

Q

P

P

V

V

A

S

L

F

L

L

C

L

G

R

E

E

-

L

-

Q

-

D

-

K

-

V

-

P

-

Q

-

L

D

P

G

G

F

F

D

S

N

W

V

I

F

K

P

P

P

-

E

-

Q

-

R

-

A

-

T

C

V

I

N

S

P

S

A

A

H

S

V

I

H

V

L

Y

F

I

G

G

I

L

R

R

S

D

L

V

D
|
D

P

P

G

P

E

E

R

H

R

F

L

I

L

L

R

K

A

N

R

Y

G

D

V

I

D

Q

V

Y

V

F

D

S

M

M

R

R

L

D

I

I

D

D

E

R

H

L

G

G

V

I

G

Q

V

K

H

V

M

M

R

E

R

R

I

T

I

F

E

D

R

L

-

L

V

I

R

G

Q

K

A

R

G

Q

G

R

H

P

L

I

H

H

V

L

S

S

L

F

D
|
D

V

I

D
|
D

F

A

L

F

D

D

P

P

A

T

I

L

A

A

P

P

A

A

V

T

G

G

T

T

A

P

V

V

L

V

G

G

G

G

A

L

T

T

Y

Y

R

R

E

E

A

G

H

M

L

Y

I

I

M

A

E

E

M

E

L

I

H

H

D

N

A

T

G

G

V

L

V

L

G

S

S

A

L

L

D

D

V

L

V

V

E
|
E

L

V

N

N

P

P

F

Q

L

L

-

A

-

T

-

S

-

E

D

E

E

E

R

A

G

K

K

T

S

T

A

A

L

N

L

L

L

A

V

V

D

D

L

V

V

I

A

A

S

S

L

S

F

F

G

G

R

Q

H120 (= H97) binding
D143 (= D124) binding ; binding
H145 (= H126) binding
HADIN 145:149 (≠ HGDFN 126:130) binding
D147 (= D128) binding
SGN 156:158 (= SGN 137:139) binding
D202 (= D180) binding
D251 (= D228) binding ; binding
D253 (= D230) binding
E296 (= E273) binding

6ss2AAA of arginase-2 in complex with the inhibitory human antigen-binding fragment fab c0021158' (see paper)
38% identity, 95% coverage: 1:298/315 of query aligns to 5:304/320 of 6ss2AAA

query
sites
6ss2AAA

V

V

E

A

I

V

I

I

G

G

V

A

P

P

I

F

E

S

A

Q

G

G

A

Q

G

K

H

R

P

K

G

G

A

V

L

E

M

H

G

G

P

P

A

A

A

A

L

I

R

R

T

E

A

A

G

G

L

L

V

M

R

K

A

R

L

L

R

S

E

S

L

L

G

G

H

C

E

H

V

L

R

K

D

D

L

F

G

G

D

D

L

L

G

S

-

F

-

T

-

P

-

V

-

P

-

K

-

D

-

D

-

L

R

Y

G

N

D

N

L

L

T

I

P

V

A

N

P

P

W

R

S

S

E

V

G

G

D

-

A

L

A

A

T

N

A

Q

R

E

L

L

A

A

E

E

I

V

T

V

A

-

W

-

S

S

R

R

A

A

L

V

A

S

D

D

R

-

S

-

Y

-

A

-

M

-

M

-

Q

-

A

-

G

G

G

Y

V

S

P

C

V

V

F

T

L

L

G

G

G

G

D

D

H
|
H

S

S

L

L

S

A

M

I

G

G

S

T

V

I

T

S

G

G

V

H

A

A

R

R

H

H

C

C

R

P

E

D

T

-

G

-

K

-

P

-

L

L

F

C

V

V

L

V

W

W

L

V

D
|
D

A

A

H
|
H

G

A

D
|
D

F

I

N

N

T

T

P

P

H

L

T

T

S

T

P

S

S

S

G

G

N

N

M

L

H

H

G

G

M

Q

P

P

V

V

A

S

L

F

L

L

C

L

G

R

E

E

-

L

-

Q

-

D

-

K

-

V

-

P

-

Q

-

L

D

P

G

G

F

F

D

S

N

W

V

I

F

K

P

P

P

-

E

-

Q

-

R

-

A

-

T

C

V

I

N

S

P

S

A

A

H

S

V

I

H

V

L

Y

F

I

G

G

I

L

R

R

S

D

L

V

D

D

P

P

G

P

E

E

R

H

R

F

L

I

L

L

R

K

A

N

R

Y

G

D

V

I

D

Q

V

Y

V

F

D

S

M

M

R

R

L

D

I

I

D

D

E

R

H

L

G

G

V

I

G

Q

V

K

H

V

M

M

R

E

R

R

I

T

I

F

E

D

R

L

-

L

V

I

R

G

Q

K

A

R

G

Q

G

R

H

P

L

I

H

H

V

L

S

S

L

F

D
|
D

V

I

D
|
D

F

A

L

F

D

D

P

P

A

T

I

L

A

A

P

P

A

A

V

T

G

G

T

T

A

P

V

V

L

V

G

G

G

G

A

L

T

T

Y

Y

R

R

E

E

A

G

H

M

L

Y

I

I

M

A

E

E

M

E

L

I

H

H

D

N

A

T

G

G

V

L

V

L

G

S

S

A

L

L

D

D

V

L

V

V

E

E

L

V

N

N

P

P

F

Q

L

L

-

A

-

T

-

S

-

E

D

E

E

E

R

A

G

K

K

T

S

T

A

A

L

N

L

L

L

A

V

V

D

D

L

V

V

I

A

A

S

S

L

S

F

F

G

G

R

Q

binding manganese (ii) ion: H99 (= H97), D122 (= D124), D122 (= D124), H124 (= H126), D126 (= D128), D230 (= D228), D230 (= D228), D232 (= D230)

6q39A Complex of arginase 2 with example 49 (see paper)
38% identity, 95% coverage: 1:298/315 of query aligns to 3:302/306 of 6q39A

query
sites
6q39A

V

V

E

A

I

V

I

I

G

G

V

A

P

P

I

F

E

S

A

Q

G

G

A

Q

G

K

H

R

P

K

G

G

A

V

L

E

M

H

G

G

P

P

A

A

A

A

L

I

R

R

T

E

A

A

G

G

L

L

V

M

R

K

A

R

L

L

R

S

E

S

L

L

G

G

H

C

E

H

V

L

R

K

D

D

L

F

G

G

D

D

L

L

G

S

-

F

-

T

-

P

-

V

-

P

-

K

-

D

-

D

-

L

R

Y

G

N

D

N

L

L

T

I

P

V

A

N

P

P

W

R

S

S

E

V

G

G

D

-

A

L

A

A

T

N

A

Q

R

E

L

L

A

A

E

E

I

V

T

V

A

-

W

-

S

S

R

R

A

A

L

V

A

S

D

D

R

-

S

-

Y

-

A

-

M

-

M

-

Q

-

A

-

G

G

G

Y

V

S

P

C

V

V

F

T

L

L

G

G

G

G

D

D

H
|
H

S

S

L

L

S

A

M

I

G

G

S

T

V

I

T

S

G

G

V

H

A

A

R

R

H

H

C

C

R

P

E

D

T

-

G

-

K

-

P

-

L

L

F

C

V

V

L

V

W

W

L

V

D
|
D

A

A

H
|
H

G

A

D
|
D

F

I

N
|
N

T

T

P

P

H

L

T

T

S

T

P

S

S
|
S

G

G

N

N

M

L

H
|
H

G
|
G

M

Q

P

P

V

V

A

S

L

F

L

L

C

L

G

R

E

E

-

L

-

Q

-

D

-

K

-

V

-

P

-

Q

-

L

D

P

G

G

F

F

D

S

N

W

V

I

F

K

P

P

P

-

E

-

Q

-

R

-

A

-

T

C

V

I

N

S

P

S

A

A

H

S

V

I

H

V

L

Y

F

I

G

G

I

L

R

R

S
x
D

L

V

D
|
D

P

P

G

P

E
|
E

R

H

R

F

L

I

L

L

R

K

A

N

R

Y

G

D

V

I

D

Q

V

Y

V

F

D

S

M

M

R

R

L

D

I

I

D

D

E

R

H

L

G

G

V

I

G

Q

V

K

H

V

M

M

R

E

R

R

I

T

I

F

E

D

R

L

-

L

V

I

R

G

Q

K

A

R

G

Q

G

R

H

P

L

I

H

H

V

L

S

S

L

F

D
|
D

V

I

D
|
D

F

A

L

F

D

D

P

P

A

T

I

L

A

A

P

P

A

A

V

T

G

G

T
|
T

A

P

V

V

L

V

G

G

G

G

A

L

T

T

Y

Y

R

R

E

E

A

G

H

M

L

Y

I

I

M

A

E

E

M

E

L

I

H

H

D

N

A

T

G

G

V

L

V

L

G

S

S

A

L

L

D

D

V

L

V

V

E
|
E

L

V

N

N

P

P

F

Q

L

L

-

A

-

T

-

S

-

E

D

E

E

E

R

A

G

K

K

T

S

T

A

A

L

N

L

L

L

A

V

V

D

D

L

V

V

I

A

A

S

S

L

S

F

F

G

G

R

Q

active site: H97 (= H97), D120 (= D124), H122 (= H126), D124 (= D128), H137 (= H141), D228 (= D228), D230 (= D230), E273 (= E273)
binding 3-[(3~{S},4~{R})-4-azanyl-4-carboxy-1-[[(2~{S})-piperidin-2-yl]methyl]pyrrolidin-3-yl]propyl-tris(oxidanyl)boranuide: H97 (= H97), D120 (= D124), H122 (= H126), D124 (= D128), N126 (= N130), S133 (= S137), H137 (= H141), G138 (= G142), D177 (≠ S178), D179 (= D180), E182 (= E183), D228 (= D228), D230 (= D230), T242 (= T242), E273 (= E273)
binding manganese (ii) ion: H97 (= H97), D120 (= D124), D120 (= D124), H122 (= H126), D124 (= D128), D228 (= D228), D228 (= D228), D230 (= D230)

6q37A Complex of arginase 2 with example 23 (see paper)
38% identity, 95% coverage: 1:298/315 of query aligns to 3:302/306 of 6q37A

query
sites
6q37A

V

V

E

A

I

V

I

I

G

G

V

A

P

P

I

F

E

S

A

Q

G

G

A

Q

G

K

H

R

P

K

G

G

A