SitesBLAST

2cnbA Trypanosoma brucei udp-galactose-4-epimerase in ternary complex with NAD and the substrate analogue udp-4-deoxy-4- fluoro-alpha-d-galactose (see paper)
43% identity, 95% coverage: 7:329/339 of query aligns to 5:365/366 of 2cnbA

query
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2cnbA

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active site: S144 (≠ T121), A145 (= A122), A146 (≠ N123), Y169 (= Y145), K173 (= K149)
binding nicotinamide-adenine-dinucleotide: G9 (= G11), A11 (= A13), G12 (= G14), Y13 (= Y15), I14 (≠ V16), D34 (= D35), S35 (≠ N36), L36 (= L37), V37 (≠ R38), G38 (≠ Q39), G76 (≠ A54), D77 (= D55), V78 (≠ L56), M100 (≠ F77), C101 (≠ A78), A102 (= A79), L104 (≠ S81), N119 (≠ G96), S142 (= S119), S143 (= S120), S144 (≠ T121), Y169 (= Y145), K173 (= K149), Y196 (= Y172), F197 (= F173), N198 (= N174), A199 (= A175), H217 (= H193)
binding uridine-5'-diphosphate-4-deoxy-4-fluoro-alpha-d-galactose: L104 (≠ S81), V106 (= V83), S144 (≠ T121), A145 (= A122), Y169 (= Y145), Y196 (= Y172), F197 (= F173), N198 (= N174), T216 (= T192), H217 (= H193), L218 (= L194), M237 (≠ I209), P238 (≠ E210), I239 (= I211), F240 (= F212), C251 (= C223), R253 (= R225), V297 (= V261), R320 (= R284), D323 (= D287)

Q9SA77 UDP-arabinose 4-epimerase 1; UDP-D-xylose 4-epimerase 1; EC 5.1.3.5 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
40% identity, 95% coverage: 7:329/339 of query aligns to 73:409/419 of Q9SA77

query
sites
Q9SA77

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G275 (≠ P196) mutation to D: In mur4-1; 50% reduction in L-Ara in cell wall material.
R304 (= R225) mutation to Q: In mur4-3; 50% reduction in L-Ara in cell wall material.

4twrA Structure of udp-glucose 4-epimerase from brucella abortus
44% identity, 93% coverage: 5:318/339 of query aligns to 2:316/325 of 4twrA

query
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4twrA

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active site: S117 (≠ T121), C118 (≠ A122), A119 (≠ N123), Y141 (= Y145), K145 (= K149), H184 (= H188), H189 (= H193)
binding nicotinamide-adenine-dinucleotide: G8 (= G11), A10 (= A13), G11 (= G14), F12 (≠ Y15), I13 (≠ V16), D32 (= D35), N33 (= N36), L34 (= L37), S35 (≠ R38), T36 (≠ Q39), G37 (= G40), G50 (≠ A54), D51 (= D55), I52 (≠ L56), F73 (= F77), A74 (= A78), A75 (= A79), S76 (≠ L80), N92 (≠ G96), S115 (= S119), S116 (= S120), S117 (≠ T121), Y141 (= Y145), K145 (= K149), Y168 (= Y172), F169 (= F173), N170 (= N174), A171 (= A175), H184 (= H188), H189 (= H193)
binding zinc ion: E182 (= E186), H184 (= H188), E187 (= E191), H189 (= H193)

7xpoA Crystal structure of udp-glc/glcnac 4-epimerase with NAD/udp-glc
40% identity, 96% coverage: 5:331/339 of query aligns to 2:344/344 of 7xpoA

query
sites
7xpoA

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N

P

P

Y
|
Y

G

G

E

R

S

T

K
|
K

F

L

F

V

I

I

E

E

-

D

-

I

-

C

R

R

A

D

L

V

H

H

W

R

A

S

D

D

R

P

C

-

H

-

G

D

L

W

R

K

S

I

A

I

C

L

L

L

R

R

Y
|
Y

F
|
F

N
|
N

A
x
P

A

V

G

G

A

A

H

H

P

P

N

S

G

G

I

Y

I

I

G

G

E

E

D

D

-

P

H

C

D

G

P

V

E

P

T
x
N

H
x
N

L
|
L

I

M

P

P

L

Y

V

V

L

Q

D

Q

A

V

A

A

S

V

G

G

R

R

R

L

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P

H

H

I
x
L

E
x
T

I
x
V

F
x
Y

G

G

D

T

D

D

Y

Y

P

S

T

T

P

K

D

D

G

G

T

T

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x
G

V

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R
|
R

D

D

Y
|
Y

V

I

H

H

V

V

C

V

D

D

L

L

A

A

D

D

A

G

H

H

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I

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A

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A

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L

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R

A

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L

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Y

T

E

R

D

S

S

V

D

R

K

-

I

-

G

-

C

-

E

-

V

F

Y

N

N

L

L

G

G

N

T

G

G

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K

G

G

Y

T

S

S

V
|
V

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L

E

E

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M

I

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A

A

A

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F

E

E

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A

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G

L

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K

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P

P

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A

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D

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A

A

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M

I

L

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I

Y

A

A

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A

S

T

E

A

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I

A

R

E

G

K

E

E

L

L

G

K

W

W

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K

P

A

R

K

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Y

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-

D

G

L

I

D

E

S

E

I

M

I

C

G

R

S

D

A

L

W

W

E

N

W

W

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A

R

S

R

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H

N

P

P

G

Y

G

G

Y

Y

R

A

G

G

binding nicotinamide-adenine-dinucleotide: G8 (= G11), G11 (= G14), Y12 (= Y15), I13 (≠ V16), D32 (= D35), N33 (= N36), L34 (= L37), D35 (≠ R38), N36 (≠ Q39), A37 (≠ G40), V61 (≠ A54), D62 (= D55), L63 (= L56), F84 (= F77), A85 (= A78), G86 (≠ A79), K88 (≠ S81), N103 (≠ G96), S126 (= S119), S127 (= S120), S128 (≠ T121), Y152 (= Y145), K156 (= K149), Y180 (= Y172), P183 (≠ A175)
binding uridine-5'-diphosphate-glucose: S128 (≠ T121), A129 (= A122), T130 (≠ N123), Y180 (= Y172), F181 (= F173), N182 (= N174), N201 (≠ T192), N202 (≠ H193), L203 (= L194), L218 (≠ I209), T219 (≠ E210), V220 (≠ I211), Y221 (≠ F212), G232 (≠ C223), R234 (= R225), Y236 (= Y227), V275 (= V261), R298 (= R284), D301 (= D287)

1ek6A Structure of human udp-galactose 4-epimerase complexed with nadh and udp-glucose (see paper)
41% identity, 96% coverage: 5:329/339 of query aligns to 3:344/346 of 1ek6A

query
sites
1ek6A

Q

E

T

K

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V

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L

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T

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G

G

G

A

A

G
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G

Y
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Y

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x
I

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G

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S

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H

C

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D

N
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N

L
x
F

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x
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Q
x
N

G
x
A

H

F

R

R

A

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-

G

A

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P

P

S

E

D

S

-

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-

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-

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-

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-

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A

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M

A

A

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F

A
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A

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x
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E

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P

H

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Y

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Y

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x
V

N

N

T

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N

G

S

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I

N

Q

L

L

I

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E

A

I

A

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K

T

A

A

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G

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M

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S

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x
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A
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A

N
x
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Y

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-

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Y

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K

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F

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I

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-

A

-

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D

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W

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N

-

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Y

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F

N
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N

A
x
P

A

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G

G

A

A

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P

A

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C

I

I

G

G

E

E

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-

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x
N

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x
N

L
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L

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A

V

A

A

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x
L

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x
G

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R

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Y
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Y

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D

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A

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-

K

E

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-

I

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Y

N

N

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R

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A

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W

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A

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-

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F

active site: S132 (≠ T121), A133 (= A122), T134 (≠ N123), Y157 (= Y145), K161 (= K149)
binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G11), G12 (= G14), Y13 (= Y15), I14 (≠ V16), D33 (= D35), N34 (= N36), F35 (≠ L37), H36 (≠ R38), N37 (≠ Q39), A38 (≠ G40), M65 (≠ A54), D66 (= D55), I67 (≠ L56), F88 (= F77), A89 (= A78), G90 (≠ A79), K92 (≠ S81), V107 (≠ G96), S130 (= S119), S131 (= S120), S132 (≠ T121), Y157 (= Y145), K161 (= K149), Y185 (= Y172), P188 (≠ A175)
binding uridine-5'-diphosphate-glucose: S132 (≠ T121), A133 (= A122), T134 (≠ N123), Y157 (= Y145), F186 (= F173), N187 (= N174), N206 (≠ T192), N207 (≠ H193), L208 (= L194), Y211 (≠ L197), L223 (≠ I209), N224 (≠ E210), V225 (≠ I211), F226 (= F212), G237 (≠ C223), R239 (= R225), Y241 (= Y227), V277 (= V261), R300 (= R284), D303 (= D287)

Q14376 UDP-glucose 4-epimerase; Galactowaldenase; UDP-N-acetylgalactosamine 4-epimerase; UDP-GalNAc 4-epimerase; UDP-N-acetylglucosamine 4-epimerase; UDP-GlcNAc 4-epimerase; UDP-galactose 4-epimerase; EC 5.1.3.2; EC 5.1.3.7 from Homo sapiens (Human) (see 13 papers)
41% identity, 96% coverage: 5:329/339 of query aligns to 3:344/348 of Q14376

query
sites
Q14376

Q

E

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K

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L

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G

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Y

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I

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D

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F

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A

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F

R

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A

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-

G

A

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I

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F

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V

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x
D

Y

Y

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Y

H

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N

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G

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A

I

A

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T

A

A

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A
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A

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T

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E

E

-

A

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Y

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K

F

F

I

I

E

E

R

E

A

M

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W

-

A

-

D

D

R

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C

C

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S

T

-

W

-

N

-
x
A

A

V

C

L

L
|
L

R

R

Y
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Y

F
|
F

N
|
N

A

P

A

T

G

G

A

A

H

H

P

A

N

S

G

G

I

C

I

I

G

G

E

E

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D

-

P

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I

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x
N

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x
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L

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A

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A

A

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G

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H

A

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x
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N

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D

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Y

P

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T

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E

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D

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R

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Y

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L

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A

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x
K

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L

-

K

E

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R

-

I

F

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N

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Y

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G

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K

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R

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x
E

G
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G

D
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D

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A

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A

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x
C

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Y

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A

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x
L

I

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E

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G
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G

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W

S

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L

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N

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S

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G

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F

GYI 12:14 (≠ GYV 14:16) binding
DNFHN 33:37 (≠ DNLRQ 35:39) binding
N34 (= N36) to S: in GALAC3; peripheral; nearly normal activity towards UDP-galactose; dbSNP:rs121908046
DI 66:67 (≠ DL 55:56) binding
F88 (= F77) binding
G90 (≠ A79) to E: in GALAC3; 800-fold decrease in UDP-galactose epimerization activity; dbSNP:rs28940882
K92 (≠ S81) binding
V94 (= V83) to M: in GALAC3; generalized; 30-fold decrease in UDP-galactose epimerization activity; 2-fold decrease in affinity for UDP-galactose; 24% of normal activity with respect to UDP-N-acetylgalactosamine; dbSNP:rs121908047
D103 (≠ L92) to G: in GALAC3; 7-fold decrease in UDP-galactose epimerization activity; very mild decrease in activity towards UDP-N-acetylgalactosamine; dbSNP:rs28940883
S132 (≠ T121) mutation to A: Loss of activity.
SAT 132:134 (≠ TAN 121:123) binding
Y157 (= Y145) active site, Proton acceptor; mutation to F: Loss of activity.
K161 (= K149) binding
A180 (vs. gap) to V: in dbSNP:rs3204468
L183 (= L170) to P: in GALAC3; peripheral; 3-fold decrease in UDP-galactose epimerization activity; dbSNP:rs121908045
Y185 (= Y172) binding
YFN 185:187 (= YFN 172:174) binding
NNL 206:208 (≠ THL 192:194) binding
NVF 224:226 (≠ EIF 210:212) binding
R239 (= R225) binding
K257 (≠ A243) to R: in GALAC3; 7-fold decrease in UDP-galactose epimerization activity; does not affect affinity for UDP-galactose; dbSNP:rs28940884
REGD 300:303 (≠ RAGD 284:287) binding
C307 (≠ L291) mutation to Y: No effect on activity towards UDP-galactose. Loss of activity towards UDP-N-acetylgalactosamine.
L313 (≠ R297) to M: in GALAC3; 6-fold decrease in UDP-galactose epimerization activity; very mild decrease in activity towards UDP-N-acetylgalactosamine; dbSNP:rs3180383
G319 (= G303) to E: in GALAC3; nearly normal activity towards UDP-galactose; mild impairment under conditions of substrate limitation; dbSNP:rs28940885
R335 (≠ E320) to H: in GALAC3; 2-fold decrease in UDP-galactose epimerization activity; dbSNP:rs368637540

1hzjA Human udp-galactose 4-epimerase: accommodation of udp-n- acetylglucosamine within the active site (see paper)
41% identity, 96% coverage: 5:329/339 of query aligns to 2:343/345 of 1hzjA

query
sites
1hzjA

Q

E

T

K

V

V

L

L

V

V

T

T

G
|
G

G

G

A

A

G
|
G

Y
|
Y

V
x
I

G

G

S

S

H

H

C

T

V

V

A

L

E

E

L

L

D

E

R

A

G

G

F

Y

R

L

V

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V

V

L

I

D
|
D

N
|
N

L
x
F

R
x
H

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x
N

G
x
A

H

F

R

R

A

G

-

G

A

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P

S

E

D

S

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-

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-

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-

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F

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A

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A

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A

A

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F
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F

A
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A

A
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G

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x
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V

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G

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H

L

L

D

Y

Y

L

Y

H

R

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x
V

N

N

T

L

V

T

N

G

S

T

L

I

N

Q

L

L

I

L

R

E

A

I

A

M

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K

T

A

A

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G

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V

R

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K

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S

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x
S

A
|
A

N
x
T

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F

Y

G

G

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N

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P

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Y

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P

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L

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D

E

E

-

A

E

H

T

P

T

T

I

G

D

G

P

C

G

T

S

N

P

P

Y
|
Y

G

G

E

K

S

S

K
|
K

F

F

I

I

E

E

R

E

A

M

L

I

H

R

W

-

A

-

D

D

R

L

C

C

H

Q

G

A

L

D

R

K

S

T

-

W

-

N

-

V

A

V

C

L

L

L

R

R

Y
|
Y

F

F

N
|
N

A
x
P

A

T

G

G

A

A

H

H

P

A

N

S

G

G

I

C

I

I

G

G

E

E

D

D

-

P

H

Q

D

G

P

I

E

P

T
x
N

H
x
N

L
|
L

I

M

P

P

L

Y

V

V

L

S

D

Q

A

V

A

A

S

I

G

G

R

R

S

E

H

A

I
x
L

E
x
N

I
x
V

F
|
F

G

G

D

N

D

D

Y

Y

P

D

T

T

P

E

D

D

G

G

T

T

C
x
G

V

V

R
|
R

D

D

Y
|
Y

V

I

H

H

V

V

C

V

D

D

L

L

A

A

D

K

A

G

H

H

L

I

R

A

V

A

L

L

P

R

A

K

L

L

E

K

T

E

R

Q

-

C

-

G

S

C

V

R

R

I

F

Y

N

N

L

L

G

G

N

T

G

G

T

T

G

G

Y

Y

S

S

V
|
V

R

L

E

Q

V

M

I

V

D

Q

A

A

T

M

E

E

R

K

V

A

T

S

G

G

L

K

T

K

V

I

P

P

V

Y

K

K

V

V

G

V

P

A

R

R

R
|
R

A

E

G

G

D
|
D

P

V

A

A

M

A

L

C

I

Y

A

A

S

N

S

P

E

S

R

L

I

A

R

Q

G

E

E

E

L

L

G

G

W

W

S

T

P

A

R

A

F

L

P

-

D

G

L

L

D

D

S

R

I

M

I

C

G

E

S

D

A

L

W

W

E

R

W

W

R

Q

R

K

R

Q

H

N

P

P

G

S

G

G

Y

F

active site: S131 (≠ T121), A132 (= A122), T133 (≠ N123), Y156 (= Y145), K160 (= K149)
binding nicotinamide-adenine-dinucleotide: G8 (= G11), G11 (= G14), Y12 (= Y15), I13 (≠ V16), D32 (= D35), N33 (= N36), F34 (≠ L37), H35 (≠ R38), N36 (≠ Q39), A37 (≠ G40), M64 (≠ A54), D65 (= D55), I66 (≠ L56), F87 (= F77), A88 (= A78), G89 (≠ A79), K91 (≠ S81), V106 (≠ G96), S129 (= S119), S130 (= S120), S131 (≠ T121), Y156 (= Y145), K160 (= K149), Y184 (= Y172), P187 (≠ A175)
binding uridine-diphosphate-n-acetylglucosamine: V93 (= V83), T133 (≠ N123), Y156 (= Y145), N186 (= N174), N205 (≠ T192), N206 (≠ H193), L207 (= L194), L222 (≠ I209), N223 (≠ E210), V224 (≠ I211), F225 (= F212), G236 (≠ C223), R238 (= R225), Y240 (= Y227), V276 (= V261), R299 (= R284), D302 (= D287)

7xpqA Crystal structure of udp-glc/glcnac 4-epimerase with NAD/udp-glcnac
40% identity, 96% coverage: 5:329/339 of query aligns to 2:342/343 of 7xpqA

query
sites
7xpqA

Q

R

T

T

V

I

L

L

V

V

T

T

G
|
G

G

G

A
|
A

G
|
G

Y
|
Y

V
x
I

G

G

S

S

H

H

C

T

V

V

A

L

E

Q

L

L

D

Q

R

Q

G

G

F

F

R

R

V

V

L

V

D
|
D

N
|
N

L
|
L

R
x
D

Q
x
N

G
x
A

H

S

R

E

A

A

V

L

P

A

-

R

-

V

-

A

-

E

-

L

-

A

-

G

-

H

-

D

-

G

S

A

D

N

A

L

A

V

F

F

V

H

E

K

A
x
V

D
|
D

L
|
L

A

R

D

D

M

R

D

H

A

A

L

L

T

V

R

D

V

I

F

F

A

S

E

S

W

H

R

R

F

F

D

E

A

A

V

V

F

I

H

H

F
|
F

A
|
A

A
x
G

L

L

S
x
K

L

A

V
|
V

G

G

E

E

S

S

M

V

R

H

D

K

P

P

H

L

L

L

Y

Y

L

Y

H

D

G
x
N

N

N

T

L

V

V

N

G

S

T

L

I

N

T

L

L

I

L

R

E

A

V

A

M

T

A

A

N

G

G

V

C

R

K

K

K

M

L

V

V

F

F

S
|
S

T
x
S

A

A

N
x
T

L

V

F

Y

G

G

T

W

P

P

E

K

R

E

Q

V

P

P

I

C

D

T

E

E

T

F

T

P

I

L

D

C

P

A

G

T

S

N

P

P

Y
|
Y

G

G

E

R

S

T

K
|
K

F

L

F

V

I

I

E

E

-

D

-

I

-

C

R

R

A

D

L

V

H

H

W

R

A

S

D

D

R

P

C

-

H

-

G

D

L

W

R

K

S

I

A

I

C

L

L

L

R

R

Y
|
Y

F
|
F

N
|
N

A
x
P

A

V

G

G

A

A

H

H

P

P

N

S

G

G

I

Y

I

I

G

G

E

E

D

D

-

P

H

C

D

G

P

V

E

P

T
x
N

H
x
N

L
|
L

I

M

P

P

L

Y

V

V

L

Q

D

Q

A

V

A

A

S

V

G

G

R

R

R

L

S

P

H

H

I
x
L

E
x
T

I
x
V

F
x
Y

G

G

D

T

D

D

Y

Y

P

S

T

T

P

K

D

D

G

G

T

T

C
x
G

V

V

R
|
R

D

D

Y
|
Y

V

I

H

H

V

V

C

V

D

D

L

L

A

A

D

D

A

G

H

H

L

I

R

A

V

A

L

L

P

R

A

K

L

L

E

Y

T

E

R

D

S

S

V

D

R

K

-

I

-

G

-

C

-

E

-

V

F

Y

N

N

L

L

G

G

N

T

G

G

T

K

G

G

Y

T

S

S

V
|
V

R

L

E

E

V

M

I

V

D

A

A

A

T

F

E

E

R

K

V

A

T

S

G

G

L

K

T

K

V

I

P

P

V

L

K

V

V

F

G

A

P

G

R

R

R
|
R

A

P

G

G

D
|
D

P

A

A

E

M

I

L

V

I

Y

A

A

S

A

S

T

E

A

R

K

I

A

R

E

G

K

E

E

L

L

G

K

W

W

S

K

P

A

R

K

F

Y

P

-

D

G

L

I

D

E

S

E

I

M

I

C

G

R

S

D

A

L

W

W

E

N

W

W

R

A

R

S

R

K

H

N

P

P

G

Y

G

G

Y

Y

binding nicotinamide-adenine-dinucleotide: G8 (= G11), A10 (= A13), G11 (= G14), Y12 (= Y15), I13 (≠ V16), D32 (= D35), N33 (= N36), L34 (= L37), D35 (≠ R38), N36 (≠ Q39), A37 (≠ G40), V61 (≠ A54), D62 (= D55), L63 (= L56), F84 (= F77), A85 (= A78), G86 (≠ A79), K88 (≠ S81), N103 (≠ G96), S126 (= S119), S127 (= S120), S128 (≠ T121), Y152 (= Y145), K156 (= K149), Y180 (= Y172), F181 (= F173), P183 (≠ A175)
binding uridine-diphosphate-n-acetylglucosamine: V90 (= V83), S128 (≠ T121), T130 (≠ N123), Y152 (= Y145), N182 (= N174), N201 (≠ T192), N202 (≠ H193), L203 (= L194), L218 (≠ I209), T219 (≠ E210), V220 (≠ I211), Y221 (≠ F212), G232 (≠ C223), R234 (= R225), Y236 (= Y227), V275 (= V261), R298 (= R284), D301 (= D287)

1i3lA Molecular basis for severe epimerase-deficiency galactosemia: x-ray structure of the human v94m-substituted udp-galactose 4-epimerase (see paper)
41% identity, 96% coverage: 5:329/339 of query aligns to 2:343/347 of 1i3lA

query
sites
1i3lA

Q

E

T

K

V

V

L

L

V

V

T

T

G
|
G

G

G

A

A

G
|
G

Y
|
Y

V
x
I

G

G

S

S

H

H

C

T

V

V

A

L

E

E

L

L

D

E

R

A

G

G

F

Y

R

L

V

P

V

V

L

I

D
|
D

N
|
N

L
x
F

R
x
H

Q
x
N

G
x
A

H

F

R

R

A

G

-

G

A

S

V

L

P

P

S

E

D

S

-

L

-

R

-

V

-

Q

-

E

-

L

-

T

-

G

-

R

-

S

A

V

A

E

F

F

V

E

E

E

A
x
M

D
|
D

L
x
I

A

L

D

D

M

Q

D

G

A

A

L

L

T

Q

R

R

V

L

F

F

A

K

E

K

W

Y

R

S