SitesBLAST
Comparing AZOBR_RS23800 AZOBR_RS23800 2-keto-4-methylthiobutyrate aminotransferase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
33% identity, 97% coverage: 3:265/270 of query aligns to 4:276/290 of 5e25A
- active site: F33 (= F32), G35 (≠ T34), K151 (= K145), E184 (= E178), L207 (= L202)
- binding 2-oxoglutaric acid: Y88 (≠ V87), K151 (= K145), T247 (≠ I237), A248 (≠ L238)
- binding pyridoxal-5'-phosphate: R52 (= R51), K151 (= K145), Y155 (= Y149), E184 (= E178), G187 (≠ V181), D188 (≠ A182), L207 (= L202), G209 (= G204), I210 (= I205), T211 (≠ R206), G246 (≠ N236), T247 (≠ I237)
5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
32% identity, 99% coverage: 4:269/270 of query aligns to 4:279/290 of 5mr0D
- active site: F32 (= F32), G34 (≠ T34), K150 (= K145), E183 (= E178), L206 (= L202)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R51), G100 (= G100), L101 (≠ V101), K150 (= K145), Y154 (= Y149), E183 (= E178), G186 (≠ V181), D187 (≠ A182), L206 (= L202), I209 (= I205), T210 (≠ R206), G245 (≠ N236), T246 (≠ I237)
2y4rA Crystal structure of 4-amino-4-deoxychorismate lyase from pseudomonas aeruginosa (see paper)
36% identity, 97% coverage: 5:266/270 of query aligns to 3:266/270 of 2y4rA
- active site: F26 (= F32), T28 (= T34), K139 (= K145), E172 (= E178), V196 (≠ L202)
- binding pyridoxal-5'-phosphate: R45 (= R51), K139 (= K145), S176 (≠ A182), V196 (≠ L202), G198 (= G204), V199 (≠ I205), N235 (= N236), S236 (vs. gap)
P19938 D-alanine aminotransferase; D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase; EC 2.6.1.21 from Bacillus sp. (strain YM-1) (see 5 papers)
28% identity, 99% coverage: 4:270/270 of query aligns to 5:276/283 of P19938
- Y32 (≠ F32) binding
- R51 (= R51) binding
- R99 (= R99) binding
- H101 (≠ V101) binding
- K146 (= K145) active site, Proton acceptor; modified: N6-(pyridoxal phosphate)lysine
- E178 (= E178) binding ; mutation to K: Loss of transaminase activity and small gain in racemase activity.
- L202 (= L202) mutation to A: Inactivates enzyme.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3daaA Crystallographic structure of d-amino acid aminotransferase inactivated by pyridoxyl-d-alanine (see paper)
28% identity, 99% coverage: 4:270/270 of query aligns to 4:275/277 of 3daaA
- active site: Y31 (≠ F32), V33 (≠ T34), K145 (= K145), E177 (= E178), L201 (= L202)
- binding n-(5'-phosphopyridoxyl)-d-alanine: Y31 (≠ F32), R50 (= R51), K145 (= K145), E177 (= E178), S180 (≠ V181), S181 (≠ A182), L201 (= L202), G203 (= G204), I204 (= I205), T205 (≠ R206), S240 (≠ N236), T241 (≠ I237)
2daaA Crystallographic structure of d-amino acid aminotransferase inactivated by d-cycloserine
28% identity, 99% coverage: 4:270/270 of query aligns to 4:275/277 of 2daaA
- active site: Y31 (≠ F32), V33 (≠ T34), K145 (= K145), E177 (= E178), L201 (= L202)
- binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: Y31 (≠ F32), V33 (≠ T34), R50 (= R51), R98 (= R99), H100 (≠ V101), K145 (= K145), E177 (= E178), S180 (≠ V181), S181 (≠ A182), N182 (= N183), L201 (= L202), G203 (= G204), I204 (= I205), T205 (≠ R206), T241 (≠ I237)
1daaA Crystallographic structure of d-amino acid aminotransferase complexed with pyridoxal-5'-phosphate (see paper)
28% identity, 99% coverage: 4:270/270 of query aligns to 4:275/277 of 1daaA
- active site: Y31 (≠ F32), V33 (≠ T34), K145 (= K145), E177 (= E178), L201 (= L202)
- binding pyridoxal-5'-phosphate: R50 (= R51), K145 (= K145), E177 (= E178), S180 (≠ V181), S181 (≠ A182), L201 (= L202), G203 (= G204), I204 (= I205), T205 (≠ R206), S240 (≠ N236), T241 (≠ I237)
3lqsA Complex structure of d-amino acid aminotransferase and 4-amino-4,5- dihydro-thiophenecarboxylic acid (adta) (see paper)
28% identity, 99% coverage: 4:270/270 of query aligns to 4:275/280 of 3lqsA
- active site: Y31 (≠ F32), V33 (≠ T34), K145 (= K145), E177 (= E178), L201 (= L202)
- binding 4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]thiophene-2-carboxylic acid: V33 (≠ T34), R50 (= R51), E177 (= E178), S180 (≠ V181), S181 (≠ A182), N182 (= N183), L201 (= L202), G203 (= G204), I204 (= I205), T205 (≠ R206), S240 (≠ N236), T241 (≠ I237), T242 (≠ L238)
1a0gB L201a mutant of d-amino acid aminotransferase complexed with pyridoxamine-5'-phosphate (see paper)
28% identity, 99% coverage: 4:270/270 of query aligns to 4:275/282 of 1a0gB
- active site: Y31 (≠ F32), V33 (≠ T34), K145 (= K145), E177 (= E178), A201 (≠ L202)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R50 (= R51), K145 (= K145), E177 (= E178), S180 (≠ V181), S181 (≠ A182), G203 (= G204), I204 (= I205), T205 (≠ R206), S240 (≠ N236), T241 (≠ I237)
7p3tB Transaminase of gamma-proteobacterium (see paper)
34% identity, 97% coverage: 3:263/270 of query aligns to 5:275/299 of 7p3tB
- binding pyridoxal-5'-phosphate: R53 (= R51), K153 (= K145), R157 (≠ Y149), E186 (= E178), S187 (= S179), A188 (≠ S180), A189 (≠ V181), S190 (≠ A182), G210 (= G204), I211 (= I205), T212 (≠ R206), T248 (≠ I237)
6xwbA Crystal structure of an r-selective transaminase from thermomyces stellatus. (see paper)
32% identity, 97% coverage: 3:264/270 of query aligns to 29:301/319 of 6xwbA
- binding pyridoxal-5'-phosphate: R77 (= R51), K178 (= K145), F185 (≠ S152), E211 (= E178), G214 (≠ V181), F215 (≠ A182), N216 (= N183), L233 (= L202), G235 (= G204), V236 (≠ I205), T237 (≠ R206), T273 (≠ N236)
6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
32% identity, 96% coverage: 4:263/270 of query aligns to 9:278/301 of 6thqB
- active site: F37 (= F32), K156 (≠ L147), E190 (= E178), L214 (= L202)
- binding pyridoxal-5'-phosphate: R60 (= R51), K156 (≠ L147), Y161 (= Y149), E190 (= E178), N195 (= N183), L214 (= L202), G216 (= G204), I217 (= I205), T218 (≠ R206), T254 (≠ I237)
- binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R51), Y97 (≠ L88), K156 (≠ L147), Y161 (= Y149), E190 (= E178), G193 (≠ V181), E194 (≠ A182), N195 (= N183), G216 (= G204), I217 (= I205), T218 (≠ R206), G253 (≠ N236), T254 (≠ I237), A255 (≠ L238)
5fr9A Structure of transaminase ata-117 arrmut11 from arthrobacter sp. Knk168 inhibited with 1-(4-bromophenyl)-2-fluoroethylamine (see paper)
30% identity, 97% coverage: 5:265/270 of query aligns to 29:301/319 of 5fr9A
- active site: Y56 (≠ F32), K177 (= K145), E210 (= E178), L232 (= L202)
- binding [4-[3-(4-bromophenyl)-3-oxidanylidene-propyl]-6-methyl-5-oxidanyl-pyridin-3-yl]methyl phosphate: R75 (= R51), K177 (= K145), E210 (= E178), G213 (≠ V181), F214 (≠ A182), L232 (= L202), G234 (= G204), I235 (= I205), T236 (≠ R206), T272 (vs. gap)
8ivpB Crystal structure of mv in complex with llp and fru from mycobacterium vanbaalenii
32% identity, 97% coverage: 3:263/270 of query aligns to 30:302/322 of 8ivpB
1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
28% identity, 97% coverage: 3:265/270 of query aligns to 4:283/304 of 1iyeA
- active site: F33 (= F32), G35 (≠ T34), K156 (≠ V144), A157 (≠ K145), E190 (= E178), L214 (= L202)
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R51), Y92 (≠ V87), Y126 (≠ P121), K156 (≠ V144), Y161 (= Y149), E190 (= E178), G193 (≠ V181), E194 (≠ A182), N195 (= N183), L214 (= L202), G216 (= G204), I217 (= I205), T218 (≠ R206), G253 (≠ N236), T254 (≠ I237), A255 (≠ L238)
1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
28% identity, 97% coverage: 3:265/270 of query aligns to 4:283/304 of 1iydA
- active site: F33 (= F32), G35 (≠ T34), K156 (≠ V144), A157 (≠ K145), E190 (= E178), L214 (= L202)
- binding glutaric acid: Y92 (≠ V87), Y126 (≠ P121), A255 (≠ L238)
- binding pyridoxal-5'-phosphate: R56 (= R51), K156 (≠ V144), Y161 (= Y149), E190 (= E178), G193 (≠ V181), E194 (≠ A182), L214 (= L202), G216 (= G204), I217 (= I205), T218 (≠ R206), T254 (≠ I237)
1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
28% identity, 97% coverage: 3:265/270 of query aligns to 4:283/304 of 1i1mA
- active site: K156 (≠ V144)
- binding 4-methyl valeric acid: Y92 (≠ V87), K156 (≠ V144), T254 (≠ I237), A255 (≠ L238)
- binding pyridoxal-5'-phosphate: R56 (= R51), K156 (≠ V144), Y161 (= Y149), E190 (= E178), G193 (≠ V181), E194 (≠ A182), L214 (= L202), G216 (= G204), I217 (= I205), T218 (≠ R206), G253 (≠ N236), T254 (≠ I237)
1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
28% identity, 97% coverage: 3:265/270 of query aligns to 4:283/304 of 1i1lA
- active site: K156 (≠ V144)
- binding 2-methylleucine: Y92 (≠ V87), K156 (≠ V144), T254 (≠ I237), A255 (≠ L238)
- binding pyridoxal-5'-phosphate: R56 (= R51), K156 (≠ V144), Y161 (= Y149), E190 (= E178), G193 (≠ V181), G216 (= G204), I217 (= I205), T218 (≠ R206), T254 (≠ I237)
6fteB Crystal structure of an (r)-selective amine transaminase from exophiala xenobiotica
29% identity, 97% coverage: 3:264/270 of query aligns to 29:302/321 of 6fteB
- active site: Y58 (≠ F32), K179 (= K145), E212 (= E178), L234 (= L202)
- binding pyridoxal-5'-phosphate: R77 (= R51), K179 (= K145), E212 (= E178), F216 (≠ A182), N217 (= N183), L234 (= L202), G236 (= G204), V237 (≠ I205), T238 (≠ R206), T274 (≠ N236)
4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
31% identity, 96% coverage: 4:263/270 of query aligns to 9:284/306 of 4whxA
Query Sequence
>AZOBR_RS23800 AZOBR_RS23800 2-keto-4-methylthiobutyrate aminotransferase
VSVVWLNGCLLPAEEARIDPADRGFTLGDGLFETIRVAGGTARHLGRHLARLADSAALLG
LPLPHDAAALAVAAEALIAAQGRVEGVLRITLTRGTGARGVLPPVDAVPTLLMTLAPAPP
PAGPVEAVIARTTRRNEHSPLSRVKSLNYLDSILARQEAATRGAGEALLLNGAGRLAESS
VANLFIVRDGRPLTPPVAEGALPGIRRALILERGDAGEAPLSVTDLLGAEEAFLTNILGL
RPLLRVDGRAIGAGTVGPVTAALLKDIAAE
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory