SitesBLAST

S109 (≠ E52) to W: in D2HGA1; unknown pathological significance; significant loss of catalytic activity; dbSNP:rs142050154
N127 (≠ T70) to K: in D2HGA1; unknown pathological significance; complete loss of catalytic activity; dbSNP:rs762857195
G131 (≠ A74) to V: in D2HGA1; unknown pathological significance; complete loss of catalytic activity
I147 (≠ L88) to S: in D2HGA1; severe phenotype; unknown pathological significance; almost complete loss of catalytic activity; dbSNP:rs121434361
M153 (≠ S94) to T: in D2HGA1; unknown pathological significance; significant loss of catalytic activity; to V: in D2HGA1; unknown pathological significance; significant loss of catalytic activity; dbSNP:rs1432270139
C172 (≠ V113) to Y: in D2HGA1; unknown pathological significance; moderate reduction in catalytic activity; dbSNP:rs773735172
P189 (≠ G130) to L: in D2HGA1; unknown pathological significance; almost complete loss of catalytic activity; dbSNP:rs587783517
A205 (= A146) to V: in D2HGA1; unknown pathological significance; almost complete loss of catalytic activity; dbSNP:rs750889931
A231 (= A172) to V: in D2HGA1; unknown pathological significance; significant loss of catalytic activity
G233 (= G174) to S: in D2HGA1; unknown pathological significance; no effect on catalytic activity; dbSNP:rs374535734
D375 (= D367) to Y: in D2HGA1; unknown pathological significance; moderate reduction in catalytic activity; dbSNP:rs267606759
R386 (= R378) binding ; binding ; binding ; mutation to A: Loss of catalytic activity.
T390 (≠ V385) binding ; binding ; mutation to A: Significantly reduced catalytic activity.
V399 (= V395) to M: in D2HGA1; unknown pathological significance; moderate reduction in catalytic activity; dbSNP:rs746519212
K401 (≠ I397) binding ; binding ; mutation to A: Loss of catalytic activity.
R419 (≠ Q415) to H: in D2HGA1; unknown pathological significance; moderate reduction in catalytic activity; dbSNP:rs199908032
A426 (≠ Y423) to T: in D2HGA1; unknown pathological significance; no effect on catalytic activity; dbSNP:rs146578303
H434 (= H431) binding ; mutation to A: Loss of catalytic activity.
G436 (≠ L433) to V: slight reduction in catalytic activity
N439 (= N436) to D: in D2HGA1; mild phenotype; unknown pathological significance; moderate reduction in catalytic activity; dbSNP:rs121434362
H441 (= H438) binding ; mutation to A: Loss of catalytic activity.
N443 (≠ V440) binding ; mutation to A: Significantly reduced catalytic activity.
V444 (≠ F441) to A: in D2HGA1; severe phenotype; unknown pathological significance; significant reduction in catalytic activity; dbSNP:rs121434360
A446 (vs. gap) to V: in D2HGA1; unknown pathological significance; moderate reduction in catalytic activity; dbSNP:rs746956176
E475 (= E476) binding ; mutation to A: Loss of catalytic activity.
H476 (= H477) binding ; binding ; binding ; mutation to A: Loss of catalytic activity.
G477 (= G478) to R: in D2HGA1; unknown pathological significance; almost complete loss of catalytic activity; dbSNP:rs1453924640

Sites not aligning to the query:

15 R → G: in dbSNP:rs4675887
169:521 natural variant: Missing (in D2HGA1; unknown pathological significance)
400:521 natural variant: Missing (in D2HGA1; unknown pathological significance; complete loss of catalytic activity)

6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
24% identity, 52% coverage: 11:514/962 of query aligns to 16:461/467 of 6lpnB

query
sites
6lpnB

A

A

L

F

R

E

E

R

F

I

M

V

P

P

D

G

E

G

R

-

L

V

V

V

T

T

D

D

P

P

L

E

R

A

T

L

L

Q

A

A

Y

P

G

N

T

V

D

D

G
x
W

-

L

S

R

F

T

Y

L

R

R

L

G

I

C

P

S

K

K

I

V

V

L

A

L

I

R

V

P

E

R

A

T

E

S

G

E

E

E

V

V

V

S

R

H

L

I

L

L

N

R

L

H

C

C

R

H

E

E

L

R

K

N

T

L

P

A

V

V

T

N

F
x
P

R
x
Q

A
x
G

G
x
N

T
|
T

S
x
G

L
x
M

S

V

G

G

Q
x
G

A
x
S

V

V

-

P

-

V

T

F

D

D

S

E

V

I

L

I

V

L

L

S

L

T

G

A

D

R

S

M

W

N

R

R

G

V

C

L

T

S

I

F

P

H

P

S

G

V

A

S

A

G

T

I

V

L

T

V

L

C

Q

Q

P

A

G

G

V

C

I

V

G

L

A

E

E

E

A

L

N

S

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R

K

Y

L

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A

E

P

E

F

R

G

D

R

F

K

I

I

M

G

P

P

L

D

D

P
x
L

A
x
G

S
x
A

I

K

A

G

T

S

A
x
C

K
x
H

I

I

G
|
G

I
x
N

A

V

A
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A

N
x
T

N

N

A
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A

S
x
G

G
|
G

M

L

C

R

C

F

G

L

T

R

A

Y

Q

G

N

S

S

L

Y

H

R

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T

T

L

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A

L

S

G

M

L

R

E

L

V

V

V

L

L

A

A

D

D

G

G

T

T

L

V

L

L

D

D

T

C

G

-

D

-

A

-

S

-

R

-

A

-

F

-

A

-

V

-

S

-

H

-

G

-

A

-

L

-

S

-

G

-

L

L

S

T

D

S

L

L

A

R

A

-

R

-

T

-

R

-

A

-

D

-

K

-

L

-

A

-

E

-

R

-

I

-

R

-

N

-

K

-

F

-

R

-

I

-

K

K

N

D

T

N

T

T

G

G

Y

Y

S

D

L

L

N

-

A

-

L

-

V

-

D

-

F

-

E

-

P

-

V

-

E

-

I

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L

-

Q

K

H

Q

L

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M

F

I

I

G

G

S

S

E
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E

G
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G

T

T

L

L

G

G

F
x
I

L
x
I

S

T

E

T

I

V

T

S

Y

I

N

L

T

C

V

P

P

P

E

K

H

P

A

R

H

A

K

V

A

N

N

V

A

A

L

F

L

L

M

G

Y

C

P

P

D

G

I

F

G

A

E

E

A

V

C

L

R

Q

A

T

V

F

A

S

L

T

M

C

K

K

P

G

T

M

-

L

-

G

-

E

P

I

V

L

S

S

A

A

V

F

E

E

L

F

M

M

D

D

R

A

A

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C

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Q

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L

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V

E

G

G

R

K

H

P

L

G

H

M

L

P

A

D

S

F

P

I

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G

Q

G

-

L

-

G

-

P

E

D

S

A

P

S

F

A

Y

L

V

L

L

V

I

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E

I

T

R

S

G

G

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D

N

A

A

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L

D

D

A

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N

K

I

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A

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Q

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D

P

G

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F

M

T

A

T

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D

D

A

Q

K

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V

E

K

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M

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L

W

W

K

A

I

L

R

R

K

E

G

R

L

I

F

-

P

-

A

-

V
x
T

G

E

A

A

M

L

R

S

K

R

V

D

G

G

T

Y

T

-

V

V

I

Y

I

K

E

Y

D

D

V

L

A

S

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L

P

P

L

V

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R

R

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A

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E

D

A

I

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V

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D

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P

G

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A

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I

V

I

G

F

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G

G

H
|
H

A

L

L

G

E

D

G

G

N

N

L

L

H

H

F

L

V

N

F

V

T

T

-

A

Q

E

A

A

F

F

D

S

T

P

D

S

E

L

V

A

D

A

R

L

Y

E

R

P

R

H

F

V

M

Y

D

E

A

W

V

T

C

A

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G

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Q

V

-

R

-

K

-

Y

-

D

Q

G

G

S

S

L

V

K

S

A

A

E
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E

H
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H

G

G

T

V

G

G

R

F

N

R

M

K

A

R

P

D

F

V

V

L

E

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M

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E

S

W

K

G

P

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P

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G

A

A

Y

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G

Q

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L

M

M

K

Q

E

Q

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K

A

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D

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G

G

L

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N
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N

P

P

binding flavin-adenine dinucleotide: W40 (≠ G36), P76 (≠ F71), Q77 (≠ R72), G78 (≠ A73), G79 (≠ A74), N80 (≠ G75), T81 (= T76), G82 (≠ S77), M83 (≠ L78), G86 (≠ Q81), S87 (≠ A82), L140 (≠ P133), G141 (≠ A134), A142 (≠ S135), C146 (≠ A139), H147 (≠ K140), G149 (= G142), G150 (= G143), N151 (≠ I144), A153 (= A146), T154 (≠ N147), A156 (= A149), G157 (≠ S150), G158 (= G151), E207 (= E251), G208 (= G252), I212 (≠ F256), I213 (≠ L257), T338 (≠ V385), H382 (= H431), E423 (= E476), H424 (= H477), N460 (= N513)

6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
24% identity, 52% coverage: 11:514/962 of query aligns to 15:460/466 of 6lpxA

query
sites
6lpxA

A

A

L

F

R

E

E

R

F

I

M

V

P

P

D

G

E

G

R

-

L

V

V

V

T

T

D

D

P

P

L

E

R

A

T

L

L

Q

A

A

Y

P

G

N

T

V

D

D

G
x
W

-

L

S

R

F

T

Y

L

R

R

L

G

I

C

P

S

K

K

I

V

V

L

A

L

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P

E

R

A

T

E

S

G

E

E

E

V

V

V

S

R

H

L

I

L

L

N

R

L

H

C

C

R

H

E

E

L

R

K

N

T

L

P

A

V

V

T

N

F
x
P

R
x
Q

A
x
G

G
x
N

T
|
T

S
x
G

L
x
M

S

V

G

G

Q
x
G

A
x
S

V

V

-

P

-

V

T

F

D

D

S

E

V

I

L

I

V

L

L

S

L

T

G

A

D

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N

R

R

G

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C

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F

P

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P

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A

S

A

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C

Q

Q

P

A

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G

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C

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G

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E

E

A

L

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S

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R

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G

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P

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D

D

P
x
L

A
x
G

S
x
A

I

K

A

G

T

S

A
x
C

K
x
H

I

I

G
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G

I
x
N

A

V

A
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A

N
x
T

N

N

A
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A

S
x
G

G
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G

M

L

C

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F

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S

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V

V

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L

A

A

D

D

G

G

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T

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V

L

L

D

D

T

C

G

-

D

-

A

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S

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R

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A

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F

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A

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V

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S

-

H

-

G

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A

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L

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S

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G

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L

S

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S

L

L

A

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A

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R

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T

-

R

-

A

-

D

-

K

-

L

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A

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E

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R

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I

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R

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N

-

K

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F

-

R

-

I

-

K

K

N

D

T

N

T

T

G

G

Y

Y

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L

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N

-

A

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L

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Y

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D

D

A

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K

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M

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W

W

K

A

I

L

R
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R

K

E

G

R

L

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F

-

P

-

A

-

V
x
T

G

E

A

A

M

L

R

S

K

R

V

D

G

G

T

Y

T

-

V

V

I

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I
x
K

E

Y

D

D

V

L

A

S

Y

L

P

P

L

V

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E

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R

L

L

A

Y

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D

A

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T

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D

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L

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A

A

M

R

F

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L

L

K

G

F

P

G

H

Y

A

T

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A

V

I

V

I

G

F
x
Y

G

G

H
|
H

A

L

L

G

E

D

G

G

N

N

L

L

H
|
H

F

L

V
x
N

F

V

T

T

-

A

Q

E

A

A

F

F

D

S

T

P

D

S

E

L

V

A

D

A

R

L

Y

E

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H

F

V

M

Y

D

E

A

W

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C

A

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R

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K

-

Y

-

D

Q

G

G

S

S

L

V

K

S

A

A

E
|
E

H
|
H

G

G

T

V

G

G

R

F

N

R

M

K

A

R

P

D

F

V

V

L

E

G

M

Y

E

S

W

K

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P

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G

A

A

Y

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G

Q

L

L

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M

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Q

I

L

K

K

A

A

L

L

D

D

P

P

Q

K

G

G

L

I

L

L

N
|
N

P

P

binding 2-oxoglutaric acid: R333 (= R378), T337 (≠ V385), K348 (≠ I397), Y379 (≠ F429), H381 (= H431), H388 (= H438), N390 (≠ V440), H423 (= H477)
binding flavin-adenine dinucleotide: W39 (≠ G36), P75 (≠ F71), Q76 (≠ R72), G77 (≠ A73), G78 (≠ A74), N79 (≠ G75), T80 (= T76), G81 (≠ S77), M82 (≠ L78), G85 (≠ Q81), S86 (≠ A82), L139 (≠ P133), G140 (≠ A134), A141 (≠ S135), C145 (≠ A139), H146 (≠ K140), G148 (= G142), G149 (= G143), N150 (≠ I144), A152 (= A146), T153 (≠ N147), A155 (= A149), G156 (≠ S150), G157 (= G151), E206 (= E251), G207 (= G252), I211 (≠ F256), I212 (≠ L257), H381 (= H431), E422 (= E476), H423 (= H477), N459 (= N513)
binding zinc ion: H381 (= H431), H388 (= H438), E422 (= E476)

6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
24% identity, 52% coverage: 11:514/962 of query aligns to 15:460/466 of 6lpuA

query
sites
6lpuA

A

A

L

F

R

E

E

R

F

I

M

V

P

P

D

G

E

G

R

-

L

V

V

V

T

T

D

D

P

P

L

E

R

A

T

L

L

Q

A

A

Y

P

G

N

T

V

D

D

G
x
W

-

L

S

R

F

T

Y

L

R

R

L

G

I

C

P

S

K

K

I

V

V

L

A

L

I

R

V

P

E

R

A

T

E

S

G

E

E

E

V

V

V

S

R

H

L

I

L

L

N

R

L

H

C

C

R

H

E

E

L

R

K

N

T

L

P

A

V

V

T

N

F
x
P

R
x
Q

A
x
G

G
x
N

T
|
T

S
x
G

L
x
M

S

V

G

G

Q
x
G

A
x
S

V

V

-

P

-

V

T

F

D

D

S

E

V

I

L

I

V

L

L

S

L

T

G

A

D

R

S

M

W

N

R

R

G

V

C

L

T

S

I

F

P

H

P

S

G

V

A

S

A

G

T

I

V

L

T

V

L

C

Q

Q

P

A

G

G

V

C

I

V

G

L

A

E

E

E

A

L

N

S

R

R

K

Y

L

V

A

E

P

E

F

R

G

D

R

F

K

I

I

M

G

P

P

L

D

D

P
x
L

A
x
G

S
x
A

I

K

A

G

T

S

A
x
C

K
x
H

I

I

G
|
G

I
x
N

A

V

A
|
A

N
x
T

N

N

A
|
A

S
x
G

G
|
G

M

L

C

R

C

F

G

L

T

R

A

Y

Q

G

N

S

S

L

Y

H

R

G

T

T

L

V

A

L

S

G

M

L

R

E

L

V

V

V

L

L

A

A

D

D

G

G

T

T

L

V

L

L

D

D

T

C

G

-

D

-

A

-

S

-

R

-

A

-

F

-

A

-

V

-

S

-

H

-

G

-

A

-

L

-

S

-

G

-

L

L

S

T

D

S

L

L

A

R

A

-

R

-

T

-

R

-

A

-

D

-

K

-

L

-

A

-

E

-

R

-

I

-

R

-

N

-

K

-

F

-

R

-

I

-

K

K

N

D

T

N

T

T

G

G

Y

Y

S

D

L

L

N

-

A

-

L

-

V

-

D

-

F

-

E

-

P

-

V

-

E

-

I

-

L

-

Q

K

H

Q

L

L

M

F

I

I

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G

G

F
x
I

L
x
I

S

T

E

T

I

V

T

S

Y

I

N

L

T

C

V

P

P

P

E

K

H

P

A

R

H

A

K

V

A

N

N

V

A

A

L

F

L

L

M

G

Y

C

P

P

D

G

I

F

G

A

E

E

A

V

C

L

R

Q

A

T

V

F

A

S

L

T

M

C

K

K

P

G

T

M

-

L

-

G

-

E

P

I

V

L

S

S

A

A

V

F

E

E

L

F

M

M

D

D

R

A

A

V

S

C

L

M

R

Q

S

L

V

V

E

G

G

R

K

H

P

L

G

H

M

L

P

A

D

S

F

P

I

V

G

Q

G

-

L

-

G

-

P

E

D

S

A

P

S

F

A

Y

L

V

L

L

V

I

E

E

I

T

R

S

G

G

E

S

D

N

A

A

A

G

A

H

L

D

D

A

A

E

N

K

I

L

A

G

A

H

V

F

S

L

A

E

V

H

I

A

A

L

Q

G

T

S

Q

G

T

L

L

V

F

T

P

D

P

G

T

T

F

M

T

A

T

T

D

D

A

Q

K

R

L

K

G

V

E

K

S

M

Y

L

W

W

K

A

I

L

R
|
R

K

E

G

R

L

I

F

-

P

-

A

-

V
x
T

G

E

A

A

M

L

R

S

K

R

V

D

G

G

T

Y

T

-

V

V

I

Y

I
x
K

E

Y

D

D

V

L

A

S

Y

L

P

P

L

V

D

E

R

R

L

L

A

Y

E

D

A

I

T

V

V

T

E

D

L

L

Q

R

A

A

M

R

F

-

L

L

K

G

F

P

G

H

Y

A

T

K

E

H

A

V

I

V

I

G

F
x
Y

G

G

H
|
H

A

L

L

G

E

D

G

G

N

N

L

L

H
|
H

F

L

V
x
N

F

V

T

T

-

A

Q

E

A

A

F

F

D

S

T

P

D

S

E

L

V

A

D

A

R

L

Y

E

R

P

R

H

F

V

M

Y

D

E

A

W

V

T

C

A

D

G

Q

Q

V

-

R

-

K

-

Y

-

D

Q

G

G

S

S

L

V

K

S

A

A

E
|
E

H
|
H

G

G

T

V

G

G

R

F

N

R

M

K

A

R

P

D

F

V

V

L

E

G

M

Y

E

S

W

K

G

P

P

P

Q

G

A

A

Y

L

G

Q

L

L

M

M

K

Q

E

Q

I

L

K

K

A

A

L

L

D

D

P

P

Q

K

G

G

L

I

L

L

N
|
N

P

P

binding flavin-adenine dinucleotide: W39 (≠ G36), P75 (≠ F71), Q76 (≠ R72), G77 (≠ A73), G78 (≠ A74), N79 (≠ G75), T80 (= T76), G81 (≠ S77), M82 (≠ L78), G85 (≠ Q81), S86 (≠ A82), L139 (≠ P133), G140 (≠ A134), A141 (≠ S135), C145 (≠ A139), H146 (≠ K140), G148 (= G142), G149 (= G143), N150 (≠ I144), A152 (= A146), T153 (≠ N147), A155 (= A149), G156 (≠ S150), G157 (= G151), E206 (= E251), G207 (= G252), I211 (≠ F256), I212 (≠ L257), H381 (= H431), E422 (= E476), H423 (= H477), N459 (= N513)
binding (2s)-2-hydroxypentanedioic acid: M82 (≠ L78), R333 (= R378), T337 (≠ V385), K348 (≠ I397), Y379 (≠ F429), H381 (= H431), H388 (= H438), N390 (≠ V440), H423 (= H477)
binding zinc ion: H381 (= H431), H388 (= H438), E422 (= E476)

6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
24% identity, 52% coverage: 11:514/962 of query aligns to 15:460/466 of 6lpqA

query
sites
6lpqA

A

A

L

F

R

E

E

R

F

I

M

V

P

P

D

G

E

G

R

-

L

V

V

V

T

T

D

D

P

P

L

E

R

A

T

L

L

Q

A

A

Y

P

G

N

T

V

D

D

G
x
W

-

L

S

R

F

T

Y

L

R

R

L

G

I

C

P

S

K

K

I

V

V

L

A

L

I

R

V

P

E

R

A

T

E

S

G

E

E

E

V

V

V

S

R

H

L

I

L

L

N

R

L

H

C

C

R

H

E

E

L

R

K

N

T

L

P

A

V

V

T

N

F
x
P

R
x
Q

A
x
G

G
x
N

T
|
T

S
x
G

L
x
M

S

V

G

G

Q
x
G

A
x
S

V

V

-

P

-

V

T

F

D

D

S

E

V

I

L

I

V

L

L

S

L

T

G

A

D

R

S

M

W

N

R

R

G

V

C

L

T

S

I

F

P

H

P

S

G

V

A

S

A

G

T

I

V

L

T

V

L

C

Q

Q

P

A

G

G

V

C

I

V

G

L

A

E

E

E

A

L

N

S

R

R

K

Y

L

V

A

E

P

E

F

R

G

D

R

F

K

I

I

M

G

P

P

L

D

D

P
x
L

A
x
G

S
x
A

I

K

A

G

T

S

A
x
C

K
x
H

I

I

G
|
G

I
x
N

A

V

A
|
A

N
x
T

N

N

A
|
A

S
x
G

G
|
G

M

L

C

R

C

F

G

L

T

R

A

Y

Q

G

N

S

S

L

Y

H

R

G

T

T

L

V

A

L

S

G

M

L

R

E

L

V

V

V

L

L

A

A

D

D

G

G

T

T

L

V

L

L

D

D

T

C

G

-

D

-

A

-

S

-

R

-

A

-

F

-

A

-

V

-

S

-

H

-

G

-

A

-

L

-

S

-

G

-

L

L

S

T

D

S

L

L

A

R

A

-

R

-

T

-

R

-

A

-

D

-

K

-

L

-

A

-

E

-

R

-

I

-

R

-

N

-

K

-

F

-

R

-

I

-

K

K

N

D

T

N

T

T

G

G

Y

Y

S

D

L

L

N

-

A

-

L

-

V

-

D

-

F

-

E

-

P

-

V

-

E

-

I

-

L

-

Q

K

H

Q

L

L

M

F

I

I

G

G

S

S

E
|
E

G
|
G

T

T

L

L

G
|
G

F
x
I

L
x
I

S

T

E

T

I

V

T

S

Y

I

N

L

T

C

V

P

P

P

E

K

H

P

A

R

H

A

K

V

A

N

N

V

A

A

L

F

L

L

M

G

Y

C

P

P

D

G

I

F

G

A

E

E

A

V

C

L

R

Q

A

T

V

F

A

S

L

T

M

C

K

K

P

G

T

M

-

L

-

G

-

E

P

I

V

L

S

S

A

A

V

F

E

E

L

F

M

M

D

D

R

A

A

V

S

C

L

M

R

Q

S

L

V

V

E

G

G

R

K

H

P

L

G

H

M

L

P

A

D

S

F

P

I

V

G

Q

G

-

L

-

G

-

P

E

D

S

A

P

S

F

A

Y

L

V

L

L

V

I

E

E

I

T

R

S

G

G

E

S

D

N

A

A

A

G

A

H

L

D

D

A

A

E

N

K

I

L

A

G

A

H

V

F

S

L

A

E

V

H

I

A

A

L

Q

G

T

S

Q

G

T

L

L

V

F

T

P

D

P

G

T

T

F

M

T

A

T

T

D

D

A

Q

K

R

L

K

G

V

E

K

S

M

Y

L

W

W

K

A

I

L

R
|
R

K

E

G

R

L

I

F

-

P

-

A

-

V
x
T

G

E

A

A

M

L

R

S

K

R

V

D

G

G

T

Y

T

-

V

V

I

Y

I
x
K

E

Y

D

D

V

L

A

S

Y

L

P

P

L

V

D

E

R

R

L

L

A

Y

E

D

A

I

T

V

V

T

E

D

L

L

Q

R

A

A

M

R

F

-

L

L

K

G

F

P

G

H

Y

A

T

K

E

H

A

V

I

V

I

G

F
x
Y

G

G

H
|
H

A

L

L

G

E

D

G

G

N

N

L

L

H
|
H

F

L

V

N

F

V

T

T

-

A

Q

E

A

A

F

F

D

S

T

P

D

S

E

L

V

A

D

A

R

L

Y

E

R

P

R

H

F

V

M

Y

D

E

A

W

V

T

C

A

D

G

Q

Q

V

-

R

-

K

-

Y

-

D

Q

G

G

S

S

L

V

K

S

A

A

E
|
E

H
|
H

G

G

T

V

G

G

R

F

N

R

M

K

A

R

P

D

F

V

V

L

E

G

M

Y

E

S

W

K

G

P

P

P

Q

G

A

A

Y

L

G

Q

L

L

M

M

K

Q

E

Q

I

L

K

K

A

A

L

L

D

D

P

P

Q

K

G

G

L

I

L

L

N
|
N

P

P

binding flavin-adenine dinucleotide: W39 (≠ G36), P75 (≠ F71), Q76 (≠ R72), G77 (≠ A73), G78 (≠ A74), N79 (≠ G75), T80 (= T76), G81 (≠ S77), G85 (≠ Q81), S86 (≠ A82), L139 (≠ P133), G140 (≠ A134), A141 (≠ S135), C145 (≠ A139), H146 (≠ K140), G148 (= G142), G149 (= G143), N150 (≠ I144), A152 (= A146), T153 (≠ N147), A155 (= A149), G156 (≠ S150), G157 (= G151), E206 (= E251), G207 (= G252), G210 (= G255), I211 (≠ F256), I212 (≠ L257), H381 (= H431), E422 (= E476), H423 (= H477), N459 (= N513)
binding d-malate: M82 (≠ L78), R333 (= R378), T337 (≠ V385), K348 (≠ I397), Y379 (≠ F429), H381 (= H431), H388 (= H438), E422 (= E476), H423 (= H477)
binding zinc ion: H381 (= H431), H388 (= H438), E422 (= E476)

6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
24% identity, 52% coverage: 11:514/962 of query aligns to 15:460/466 of 6lppA

query
sites
6lppA

A

A

L

F

R

E

E

R

F

I

M

V

P

P

D

G

E

G

R

-

L

V

V

V

T

T

D

D

P

P

L

E

R

A

T

L

L

Q

A

A

Y

P

G

N

T

V

D

D

G
x
W

-

L

S

R

F

T

Y

L

R

R

L

G

I

C

P

S

K

K

I

V

V

L

A

L

I

R

V

P

E

R

A

T

E

S

G

E

E

E

V

V

V

S

R

H

L

I

L

L

N

R

L

H

C

C

R

H

E

E

L

R

K

N

T

L

P

A

V

V

T

N

F
x
P

R
x
Q

A
x
G

G
x
N

T
|
T

S
x
G

L
x
M

S

V

G

G

Q
x
G

A
x
S

V

V

-

P

-

V

T

F

D

D

S

E

V

I

L

I

V

L

L

S

L

T

G

A

D

R

S

M

W

N

R

R

G

V

C

L

T

S

I

F

P

H

P

S

G

V

A

S

A

G

T

I

V

L

T

V

L

C

Q

Q

P

A

G

G

V

C

I

V

G

L

A

E

E

E

A

L

N

S

R

R

K

Y

L

V

A

E

P

E

F

R

G

D

R

F

K

I

I

M

G

P

P

L

D

D

P
x
L

A
x
G

S
x
A

I

K

A

G

T

S

A
x
C

K
x
H

I

I

G
|
G

I
x
N

A

V

A
|
A

N
x
T

N

N

A
|
A

S
x
G

G
|
G

M

L

C

R

C

F

G

L

T

R

A

Y

Q

G

N

S

S

L

Y

H

R

G

T

T

L

V

A

L

S

G

M

L

R

E

L

V

V

V

L

L

A

A

D

D

G

G

T

T

L

V

L

L

D

D

T

C

G

-

D

-

A

-

S

-

R

-

A

-

F

-

A

-

V

-

S

-

H

-

G

-

A

-

L

-

S

-

G

-

L

L

S

T

D

S

L

L

A

R

A

-

R

-

T

-

R

-

A

-

D

-

K

-

L

-

A

-

E

-

R

-

I

-

R

-

N

-

K

-

F

-

R

-

I

-

K

K

N

D

T

N

T

T

G

G

Y

Y

S

D

L

L

N

-

A

-

L

-

V

-

D

-

F

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E

-

P

-

V

-

E

-

I

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-

Q

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Q

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F

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I

G

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E
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E

G
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G

T

T

L

L

G

G

F
x
I

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x
I

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T

I

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T

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Y

I

N

L

T

C

V

P

P

P

E

K

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P

A

R

H

A

K

V

A

N

N

V

A

A

L

F

L

L

M

G

Y

C

P

P

D

G

I

F

G

A

E

E

A

V

C

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Q

A

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F

A

S

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T

M

C

K

K

P

G

T

M

-

L

-

G

-

E

P

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A

A

V

F

E

E

L

F

M

M

D

D

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A

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C

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V

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G

Q

G

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L

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G

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D

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F

A

Y

L

V

L

L

V

I

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E

I

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G

G

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N

A

A

A

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A

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D

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W

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T

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I

G

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Y

G

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H

A

L

L

G

E

D

G

G

N

N

L

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H

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V
x
N

F

V

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T

-

A

Q

E

A

A

F

F

D

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P

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A

D

A

R

L

Y

E

R

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Y

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A

W

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C

A

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K

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Y

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A

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E

H
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H

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G

G

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N

R

M

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A

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V

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K

A

A

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L

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D

P

P

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K

G

G

L

I

L

L

N
|
N

P

P

binding (2R)-2-hydroxypentanedioic acid: M82 (≠ L78), R333 (= R378), T337 (≠ V385), K348 (≠ I397), Y379 (≠ F429), H381 (= H431), H388 (= H438), N390 (≠ V440), E422 (= E476), H423 (= H477)
binding flavin-adenine dinucleotide: W39 (≠ G36), P75 (≠ F71), Q76 (≠ R72), G77 (≠ A73), G78 (≠ A74), N79 (≠ G75), T80 (= T76), G81 (≠ S77), M82 (≠ L78), G85 (≠ Q81), S86 (≠ A82), L139 (≠ P133), G140 (≠ A134), A141 (≠ S135), C145 (≠ A139), H146 (≠ K140), G148 (= G142), G149 (= G143), N150 (≠ I144), A152 (= A146), T153 (≠ N147), A155 (= A149), G156 (≠ S150), G157 (= G151), E206 (= E251), G207 (= G252), I211 (≠ F256), I212 (≠ L257), H381 (= H431), E422 (= E476), H423 (= H477), N459 (= N513)
binding zinc ion: H381 (= H431), H388 (= H438), E422 (= E476)

3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
26% identity, 52% coverage: 19:518/962 of query aligns to 16:465/465 of 3pm9A

query
sites
3pm9A

D

D

E

K

R

H

L

A

V

L

T

T

D

D

P

P

L

L

R

E

T

L

L

E

A

A

Y

Y

G

I

T

T

D

E

G

E

-

R

S

N

F

L

Y

Y

R

R

L

G

I

H

P

S

K

P

I

L

V

V

A

L

I

R

V

P

E

G

A

S

E

T

G

E

E

E

V

V

R

A

L

I

L

C

N

K

L

L

C

A

R

N

E

E

L

A

K

R

T

V