SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AZOBR_RS25220 FitnessBrowser__azobra:AZOBR_RS25220 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

8wm8B Cryo-em structure of cyanobacterial nitrate/nitrite transporter nrtbcd in complex with nitrate (see paper)
32% identity, 56% coverage: 91:232/252 of query aligns to 107:251/265 of 8wm8B

query
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8wm8B

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binding nitrate ion: L203 (≠ I187), A207 (= A191)

A0A0H2ZQB9 Ergothioneine transporter EgtUBC from Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) (see paper)
29% identity, 67% coverage: 84:251/252 of query aligns to 45:212/506 of A0A0H2ZQB9

query
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A0A0H2ZQB9

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Sites not aligning to the query:

244 G→F: Disrupts ergothioneine (EGT) binding. Binds EGT 100-fold more weakly. May have a global impact on overall structure.
293 F→Y: Disrupts ergothioneine (EGT) binding.
375 E→Q: Disrupts ergothioneine (EGT) binding. Binds EGT 500-fold more weakly.
419 Y→F: Disrupts ergothioneine (EGT) binding.

Query Sequence

>AZOBR_RS25220 FitnessBrowser__azobra:AZOBR_RS25220
LGRAALYVALPVALLAAWQAAFALGYIRPILLPPPTRVGKAFVDLAASGDLFRHLGVSLL
RVLEGFAIAALVGLPLGIGIGLSRTLDRLTDLIIQLTKPIPPIAWIPLAILWFGIGEAGK
VYIIFLGAIFPILVNTIDGNRQTDHRHVELARVLEVTRRRFILQVVLPGALPNIMTGLRV
GLMVAWICVVAAELIAASSGLGYLIMDARQMSQTDQVLVGMITIGAMGKLLDVVLRAAER
RLITWKTTFSGS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory