SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AZOBR_RS25220 FitnessBrowser__azobra:AZOBR_RS25220 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

A0A0H2ZQB9 Ergothioneine transporter EgtUBC from Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) (see paper)
29% identity, 67% coverage: 84:251/252 of query aligns to 45:212/506 of A0A0H2ZQB9

query
sites
A0A0H2ZQB9

R

R

T

Y

L

H

D

E

R

K

L

L

T

A

D

D

L

W

I

V

I

L

Q

Q

L

I

T

A

-

G

-

I

-

F

K

Q

P

T

I

I

P

P

P

S

I

L

A

A

W

L

I

L

P

G

L

L

A

F

I

I

L

P

W

L

F

M

G

G

I

I

G

G

E

T

A

L

G

P

K

A

V

L

Y

T

I

A

I

L

F

V

L

I

G

Y

A

A

I

I

F

F

P

P

I

I

L

L

V

Q

N

N

T

T

I

I

D

T

G

G

N

L

R

K

Q

G

T

I

D

D

H

P

R

S

H

L

V

Q

E

E

L

A

A

G

R

I

V

A

L

F

E

G

V

M

T

T

R

R

R

W

R

E

F

R

I

L

L

K

Q

K

V

F

V

E

L

I

P

P

G

L

A

A

L

M

P

P

N

V

I

I

M

M

T

S

G

G

L

I

R

R

V

T

G

A

L

A

M

V

V

L

A

I

W

I

I

G

C

T

V

A

V

T

A

L

A

A

E

T

L

L

I

I

A

G

A

A

S

-

S

G

G

G

L

L

G

G

Y

S

L

F

I

I

M

L

D

L

A

G

R

I

Q

D

M

R

S

N

Q

N

T

A

D

S

Q

L

V

I

L

L

V

I

G

G

M

A

I

L

T

S

I

S

G

A

A

V

M

L

G

A

K

I

L

A

L

F

D

N

V

F

V

L

L

L

R

K

A

V

A

M

E

E

R

K

R

A

L

K

I

L

T

-

W

-

K

R

T

T

T

I

F

F

S

S

G

G

Sites not aligning to the query:

244 G→F: Disrupts ergothioneine (EGT) binding. Binds EGT 100-fold more weakly. May have a global impact on overall structure.
293 F→Y: Disrupts ergothioneine (EGT) binding.
375 E→Q: Disrupts ergothioneine (EGT) binding. Binds EGT 500-fold more weakly.
419 Y→F: Disrupts ergothioneine (EGT) binding.

Query Sequence

>AZOBR_RS25220 FitnessBrowser__azobra:AZOBR_RS25220
LGRAALYVALPVALLAAWQAAFALGYIRPILLPPPTRVGKAFVDLAASGDLFRHLGVSLL
RVLEGFAIAALVGLPLGIGIGLSRTLDRLTDLIIQLTKPIPPIAWIPLAILWFGIGEAGK
VYIIFLGAIFPILVNTIDGNRQTDHRHVELARVLEVTRRRFILQVVLPGALPNIMTGLRV
GLMVAWICVVAAELIAASSGLGYLIMDARQMSQTDQVLVGMITIGAMGKLLDVVLRAAER
RLITWKTTFSGS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory