SitesBLAST
Comparing AZOBR_RS25270 FitnessBrowser__azobra:AZOBR_RS25270 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3rcyF Crystal structure of mandelate racemase/muconate lactonizing enzyme- like protein from roseovarius sp. Tm1035
34% identity, 91% coverage: 20:381/397 of query aligns to 24:390/397 of 3rcyF
- active site: K165 (= K153), D167 (= D155), R175 (= R162), G208 (≠ D193), H210 (= H195), E234 (= E219), G259 (= G244), E260 (= E245), Q281 (≠ N266), A283 (≠ D268), H310 (= H295), A313 (≠ N298), L332 (≠ T318), E335 (= E321)
- binding magnesium ion: E234 (= E219), E260 (= E245)
- binding alpha-D-ribofuranose: R85 (= R80), P86 (≠ T81), P239 (= P224), A266 (≠ E251), E267 (≠ Q252)
2qq6B Crystal structure of mandelate racemase/muconate lactonizing enzyme- like protein from rubrobacter xylanophilus dsm 9941
39% identity, 71% coverage: 86:368/397 of query aligns to 85:389/396 of 2qq6B
- active site: D124 (≠ N125), K166 (= K153), D168 (= D155), D213 (= D193), H215 (= H195), E239 (= E219), G264 (= G244), E265 (= E245), M286 (≠ N266), D288 (= D268), H315 (= H295), N316 (= N296), E340 (= E321), D345 (≠ V327)
- binding magnesium ion: D213 (= D193), E239 (= E219), E265 (= E245), H315 (= H295)
Sites not aligning to the query:
5olcC Crystal structure of the 3,6-anhydro-d-galactonate cycloisomerase from zobellia galactanivorans (see paper)
32% identity, 92% coverage: 2:365/397 of query aligns to 2:348/351 of 5olcC
- active site: K148 (= K153), K150 (≠ D155), D178 (= D193), N180 (≠ H195), E204 (= E219), G229 (= G244), E230 (= E245), D253 (= D268), H280 (= H295), E304 (≠ F323), E309 (= E328)
- binding magnesium ion: D178 (= D193), E204 (= E219), E230 (= E245)
2ox4C Crystal structure of putative dehydratase from zymomonas mobilis zm4
31% identity, 92% coverage: 1:367/397 of query aligns to 2:388/397 of 2ox4C
- active site: K165 (= K153), D167 (= D155), E214 (≠ D193), H216 (= H195), E240 (= E219), G265 (= G244), E266 (= E245), Q287 (≠ N266), D289 (= D268), H316 (= H295), E341 (= E321)
- binding magnesium ion: E214 (≠ D193), E240 (= E219), E266 (= E245)
Q1NAJ2 D-mannonate dehydratase; ManD; EC 4.2.1.8 from Sphingomonas sp. (strain SKA58) (see paper)
34% identity, 92% coverage: 2:368/397 of query aligns to 3:386/403 of Q1NAJ2
- D211 (= D193) binding Mg(2+)
- E237 (= E219) binding Mg(2+)
- E263 (= E245) binding Mg(2+)
3thuA Crystal structure of an enolase from sphingomonas sp. Ska58 (efi target efi-501683) with bound mg
34% identity, 92% coverage: 2:368/397 of query aligns to 5:388/405 of 3thuA
- active site: L39 (≠ V38), E43 (= E43), W79 (≠ I78), G124 (≠ A124), R150 (≠ K153), Q152 (≠ D155), Y162 (= Y164), D213 (= D193), H215 (= H195), E239 (= E219), G264 (= G244), E265 (= E245), R286 (≠ N266), T288 (≠ D268), H315 (= H295), E342 (= E321)
- binding magnesium ion: D213 (= D193), E239 (= E219), E265 (= E245)
Sites not aligning to the query:
4e4fB Crystal structure of enolase pc1_0802 (target efi-502240) from pectobacterium carotovorum subsp. Carotovorum pc1
33% identity, 93% coverage: 1:369/397 of query aligns to 2:365/381 of 4e4fB
- active site: L37 (≠ V38), R40 (≠ K42), R148 (≠ K153), Q150 (≠ D155), D189 (= D193), H191 (= H195), E215 (= E219), G240 (= G244), E241 (= E245), V242 (≠ A246), R262 (≠ N266), T264 (≠ D268), H291 (= H295), P293 (≠ F297), E318 (= E321)
- binding magnesium ion: D189 (= D193), E215 (= E219), E241 (= E245)
Sites not aligning to the query:
4e6mA Crystal structure of putative dehydratase protein from salmonella enterica subsp. Enterica serovar typhimurium (salmonella typhimurium)
31% identity, 92% coverage: 1:365/397 of query aligns to 2:395/401 of 4e6mA
- active site: L45 (≠ Y37), G48 (≠ A40), K169 (= K153), D171 (= D155), I175 (vs. gap), E223 (≠ D193), H225 (= H195), E249 (= E219), G274 (= G244), E275 (= E245), R276 (≠ A246), Q296 (≠ N266), D298 (= D268), H325 (= H295), C327 (≠ F297), E350 (= E321), A355 (≠ L326)
- binding magnesium ion: E223 (≠ D193), E249 (= E219), E275 (= E245)
4gmeC Crystal structure of mannonate dehydratase (target efi-502209) from caulobacter crescentus cb15 complexed with magnesium and d-mannonate
32% identity, 93% coverage: 1:368/397 of query aligns to 2:386/403 of 4gmeC
- active site: L37 (≠ V38), R40 (≠ G41), D211 (= D193), H213 (= H195), E237 (= E219), G262 (= G244), E263 (= E245), I264 (≠ A246), T286 (≠ D268), H313 (= H295), A315 (≠ F297), E340 (= E321)
- binding d-mannonic acid: N38 (≠ T39), Y160 (= Y164), D211 (= D193), H213 (= H195), E237 (= E219), E263 (= E245), H313 (= H295), D317 (vs. gap), E340 (= E321)
- binding magnesium ion: D211 (= D193), E237 (= E219), E263 (= E245)
Sites not aligning to the query:
4gmeA Crystal structure of mannonate dehydratase (target efi-502209) from caulobacter crescentus cb15 complexed with magnesium and d-mannonate
32% identity, 93% coverage: 1:368/397 of query aligns to 2:386/403 of 4gmeA
- active site: L37 (≠ V38), R40 (≠ G41), T119 (≠ R121), R148 (≠ K153), Q150 (≠ D155), Y160 (= Y164), D211 (= D193), H213 (= H195), E237 (= E219), G262 (= G244), E263 (= E245), I264 (≠ A246), R284 (≠ N266), T286 (≠ D268), H313 (= H295), A315 (≠ F297), E340 (= E321)
- binding carbonate ion: R148 (≠ K153), Y160 (= Y164), D211 (= D193), E263 (= E245), E340 (= E321)
- binding magnesium ion: D211 (= D193), E237 (= E219), E263 (= E245)
Sites not aligning to the query:
Q9AAR4 D-mannonate dehydratase CC0532; ManD; EC 4.2.1.8 from Caulobacter vibrioides (strain ATCC 19089 / CIP 103742 / CB 15) (Caulobacter crescentus) (see paper)
32% identity, 93% coverage: 1:368/397 of query aligns to 2:386/403 of Q9AAR4
- D211 (= D193) binding Mg(2+)
- E237 (= E219) binding Mg(2+)
- E263 (= E245) binding Mg(2+)
2o56A Crystal structure of a member of the enolase superfamily from salmonella typhimurium
31% identity, 87% coverage: 20:366/397 of query aligns to 21:387/392 of 2o56A