SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AZOBR_RS25580 FitnessBrowser__azobra:AZOBR_RS25580 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6jb0A Crystal structure of abc transporter alpha-glycoside-binding mutant protein w287a in complex with trehalose (see paper)
45% identity, 89% coverage: 44:423/427 of query aligns to 20:400/404 of 6jb0A

query
sites
6jb0A

C

M

R

K

D

E

G

M

A

V

Q

E

E

A

W

W

A

G

R

K

S

T

G

G

N

T

E

R

V

V

R

E

F

Y

V

I

S

D

P

S

P

P

K

A

G

D

A
x
T

S

N

E

D

Q
x
R

L

L

A

A

L

L

Y

Y

Q

Q

L

Y

L

W

A

A

G

R

S

S

P

P

D

D

I

V

D

D

V

V

F

Y

Q

M

I

I

D
|
D

V

V

V

I

W

W

P

P

G

G

I

I

L

V

G

A

N

P

Y

H

F

A

I

L

D

D

L

L

K

K

D

P

R

Y

A

L

G

T

P

E

D

A

T

E

V

L

G

K

R

E

H

F

L

F

P

P

A

R

M

I

I

V

E

Q

A

N

T

T

V

I

K

R

G

G

R

K

L

L

V

T

A

S

M

L

P

P

W

F

F
|
F

A

T

D
|
D

A

A

G

G

V

I

L

L

Y

Y

A

Y

R

R

K

K

D

D

L

L

E

E

A

K

H

Y

G

G

R

Y

P

T

V

S

P

P

-

P

Q

R

T

T

W

W

E

N

E

E

L

L

Q

E

D

Q

T

M

A

A

A

E

L

R

I

V

Q

M

R

E

A

G

E

E

R

R

A

R

A

A

G

G

R

N

D

R

R

D

M

F

W

W

G

G

Y

F

V

V

W

F

Q

Q

G

G

R

K

A

P

Y
|
Y

E
|
E

G
|
G

L

L

T

T

V
x
C

N

D

A

A

L

L

E

E

W

W

I

I

A

Y

S

S

R

H

N

G

G

G

T

R

I

I

V

V

A

E

P

P

D

D

G

G

A

T

I

I

T

S

I

V

D

N

N

N

P

G

Q

R

A

A

E

L

A

A

L

L

A

N

M

R

A

A

R

H

G

G

W

W

V

V

G

G

S

R

I

I

S

A

P

P

P

Q

G

G

V

V

L

T

N

S

Y

Y

M

A

E
|
E

E

E

A

A

R

R

G

N

V

V

F

W

Q

Q

S

Q

G

G

N

N

A

S

V

L

F

F

M

M

R

R

N

N

W
|
W

P

P

Y
|
Y

A

A

W

Y

T

A

L

L

V

G

N

Q

A

A

A

E

D

G

S

S

A

P

V

I

G

R

G

G

K

K

V

F

A

G

V

V

V

T

P

V

L

L

P

P

K

K

G

A

G

S

P

A

E

D

G

A

R

P

H

N

T

A

S

A

T

T

L

L

G
|
G

Q

A

L

Q

L

L

A

M

V

V

S

S

K

A

F

Y

S

S

A

R

H

Y

A

P

D

K

E

E

A

A

A

V

D

D

L

L

A

V

L

K

Y

Y

L

L

T

A

G

S

L

Y

A

E

E

V

Q

Q

K

K

R

D

R

N

A

A

I

V

H
x
R

G
x
L

A

S

S

R

N

L

P

P

T

T

I

R

P

P

A

A

L

L

Y

Y

E

T

D

D

A

R

E

D

V

V

V

L

A

A

A

R

N

N

P

P

F

W

F

F

A

R

A

D

L

L

A

L

E

P

S

V

I

F

A

Q

N

N

A

A

V

V

N

S

R
|
R

P

P

A

S

Q

D

A

V

T

A

G

G

M

A

R

R

Y

Y

N

N

Q

Q

V

V

S

S

A

E

E

A

F

I

Y

W

A

T

N

E

V

V

H

H

E

S

V

V

L

L

S

T

G

G

R

R

Q

K

D

K

A

G

K

E

A

Q

M

A

L

V

S

R

D

D

L

L

K

E

E

A

A

R

L

I

V

R

R

R

I

I

S

L

R

R

binding alpha-D-glucopyranose: T44 (≠ A68), R47 (≠ Q71), D68 (= D92), F114 (= F138), D116 (= D140), D116 (= D140), Y171 (= Y194), E172 (= E195), G173 (= G196), C176 (≠ V199), E228 (= E251), W246 (= W269), W246 (= W269), Y248 (= Y271), G283 (= G306), G284 (= G307), R354 (= R377)
binding 1,3-propandiol: R318 (≠ H341), L319 (≠ G342)

Sites not aligning to the query:

6jahA Crystal structure of abc transporter alpha-glycoside-binding protein in complex with glucose (see paper)
45% identity, 89% coverage: 44:423/427 of query aligns to 20:400/404 of 6jahA

query
sites
6jahA

C

M

R

K

D

E

G

M

A

V

Q

E

E

A

W

W

A

G

R

K

S

T

G

G

N

T

E

R

V

V

R

E

F

Y

V

I

S

D

P

S

P

P

K

A

G

D

A
x
T

S

N

E

D

Q

R

L

L

A

A

L

L

Y

Y

Q

Q

L

Y

L

W

A

A

G

R

S

S

P

P

D

D

I

V

D

D

V

V

F

Y

Q

M

I

I

D
|
D

V

V

V

I

W

W

P

P

G

G

I

I

L

V

G

A

N

P

Y

H

F

A

I

L

D

D

L

L

K

K

D

P

R

Y

A

L

G

T

P

E

D

A

T

E

V

L

G

K

R

E

H

F

L

F

P

P

A

R

M

I

I

V

E

Q

A

N

T

T

V

I

K

R

G

G

R

K

L

L

V

T

A

S

M

L

P

P

W

F

F

F

A

T

D
|
D

A

A

G

G

V

I

L

L

Y

Y

A

Y

R

R

K

K

D

D

L

L

E

E

A

K

H

Y

G

G

R

Y

P

T

V

S

P

P

-

P

Q

R

T

T

W

W

E

N

E

E

L

L

Q

E

D

Q

T

M

A

A

A

E

L

R

I

V

Q

M

R

E

A

G

E

E

R

R

A

R

A

A

G

G

R

N

D

R

R
x
D

M

F

W
|
W

G

G

Y

F

V

V

W

F

Q

Q

G

G

R

K

A

P

Y
|
Y

E
|
E

G
|
G

L

L

T

T

V
x
C

N

D

A

A

L

L

E

E

W

W

I

I

A

Y

S

S

R

H

N

G

G

G

T

R

I

I

V

V

A

E

P

P

D

D

G

G

A

T

I

I

T

S

I

V

D

N

N

N

P

G

Q

R

A

A

E

L

A

A

L

L

A

N

M

R

A

A

R

H

G

G

W

W

V

V

G

G

S

R

I

I

S

A

P

P

P

Q

G

G

V

V

L

T

N

S

Y

Y

M

A

E
|
E

E

E

A

A

R

R

G

N

V
|
V

F

W

Q

Q

S

Q

G

G

N

N

A

S

V

L

F

F

M

M

R

R

N

N

W
|
W

P

P

Y

Y

A

A

W

Y

T

A

L

L

V

G

N

Q

A

A

A

E

D

G

S

S

A

P

V

I

G

R

G

G

K

K

V

F

A

G

V

V

V

T

P

V

L

L

P

P

K

K

G

A

G

S

P

A

E

D

G

A

R

P

H

N

T

A

S

A

T

T

L

L

G
|
G

Q
x
W

L

Q

L

L

A

M

V

V

S

S

K

A

F

Y

S

S

A

R

H

Y

A

P

D

K

E

E

A

A

A

V

D

D

L

L

A

V

L

K

Y

Y

L

L

T

A

G

S

L
x
Y

A
x
E

E

V

Q

Q

K
|
K

R

D

R

N

A

A

I

V

H

R

G

L

A

S

S

R

N

L

P

P

T

T

I

R

P

P

A

A

L

L

Y

Y

E

T

D
|
D

A
x
R

E

D

V

V

V

L

A

A

A

R

N

N

P

P

F

W

F

F

A

R

A

D

L

L

A

L

E

P

S

V

I

F

A

Q

N

N

A

A

V

V

N

S

R
|
R

P

P

A

S

Q

D

A

V

T

A

G

G

M

A

R

R

Y

Y

N

N

Q

Q

V

V

S

S

A

E

E

A

F

I

Y

W

A

T

N

E

V

V

H

H

E

S

V

V

L

L

S

T

G

G

R

R

Q

K

D

K

A

G

K

E

A

Q

M

A

L

V

S

R

D

D

L

L

K

E

E

A

A

R

L

I

V

R

R

R

I

I

S

L

R

R

binding (2S)-heptane-1,2,7-triol: Y309 (≠ L332), E310 (≠ A333), K313 (= K336), D331 (= D354), R332 (≠ A355)
binding propane-2,2-diol: D160 (≠ R183), W162 (= W185), V234 (= V257)
binding alpha-D-glucopyranose: T44 (≠ A68), D68 (= D92), D116 (= D140), Y171 (= Y194), E172 (= E195), G173 (= G196), C176 (≠ V199), E228 (= E251), W246 (= W269), G283 (= G306), G284 (= G307), W285 (≠ Q308), R354 (= R377)

6jagA Crystal structure of abc transporter alpha-glycoside-binding protein in complex with sucrose (see paper)
45% identity, 89% coverage: 44:423/427 of query aligns to 20:400/404 of 6jagA

query
sites
6jagA

C

M

R

K

D

E

G

M

A

V

Q

E

E

A

W

W

A

G

R

K

S

T

G

G

N

T

E

R

V

V

R

E

F

Y

V

I

S

D

P

S

P

P

K

A

G

D

A
x
T

S

N

E

D

Q
x
R

L

L

A

A

L

L

Y

Y

Q

Q

L

Y

L

W

A

A

G

R

S

S

P

P

D

D

I

V

D

D

V

V

F

Y

Q
x
M

I

I

D
|
D

V

V

V

I

W

W

P

P

G

G

I

I

L

V

G

A

N

P

Y

H

F

A

I

L

D

D

L

L

K

K

D

P

R

Y

A

L

G

T

P

E

D

A

T

E

V

L

G

K

R

E

H

F

L

F

P

P

A

R

M

I

I

V

E

Q

A

N

T

T

V

I

K

R

G

G

R

K

L

L

V

T

A

S

M

L

P

P

W

F

F
|
F

A

T

D
|
D

A

A

G

G

V

I

L

L

Y

Y

A

Y

R

R

K

K

D

D

L

L

E

E

A

K

H

Y

G

G

R

Y

P

T

V

S

P

P

-

P

Q

R

T

T

W

W

E

N

E

E

L

L

Q

E

D

Q

T

M

A

A

A

E

L

R

I

V

Q

M

R

E

A

G

E

E

R

R

A

R

A

A

G

G

R

N

D

R

R

D

M

F

W

W

G

G

Y

F

V

V

W

F

Q

Q

G

G

R

K

A

P

Y
|
Y

E

E

G
|
G

L

L

T

T

V
x
C

N

D

A

A

L

L

E

E

W

W

I

I

A

Y

S

S

R

H

N

G

G

G

T

R

I

I

V

V

A

E

P

P

D

D

G

G

A

T

I

I

T

S

I

V

D

N

N

N

P

G

Q

R

A

A

E

L

A

A

L

L

A

N

M

R

A

A

R

H

G

G

W

W

V

V

G

G

S

R

I

I

S

A

P

P

P

Q

G

G

V

V

L

T

N

S

Y

Y

M

A

E
|
E

E

E

A

A

R

R

G

N

V

V

F

W

Q

Q

S

Q

G

G

N

N

A

S

V

L

F

F

M

M

R

R

N

N

W
|
W

P

P

Y
|
Y

A

A

W

Y

T

A

L

L

V

G

N

Q

A

A

A

E

D

G

S

S

A

P

V

I

G

R

G

G

K

K

V

F

A

G

V

V

V

T

P

V

L

L

P

P

K

K

G

A

G

S

P

A

E

D

G

A

R

P

H

N

T

A

S

A

T

T

L

L

G
|
G

Q
x
W

L

Q

L

L

A

M

V

V

S

S

K

A

F

Y

S

S

A

R

H

Y

A

P

D

K

E

E

A

A

A

V

D

D

L

L

A

V

L

K

Y

Y

L

L

T

A

G

S

L

Y

A

E

E

V

Q

Q

K

K

R

D

R

N

A

A

I

V

H

R

G

L

A

S

S
x
R

N

L

P

P

T

T

I

R

P

P

A

A

L

L

Y

Y

E

T

D

D

A

R

E

D

V

V

V

L

A

A

A

R

N

N

P

P

F

W

F

F

A

R

A

D

L

L

A

L

E

P

S

V

I

F

A

Q

N

N

A

A

V

V

N

S

R
|
R

P

P

A

S

Q

D

A

V

T

A

G

G

M

A

R

R

Y

Y

N

N

Q

Q

V

V

S

S

A

E

E

A

F

I

Y

W

A

T

N

E

V

V

H

H

E

S

V

V

L

L

S

T

G

G

R

R

Q

K

D

K

A

G

K

E

A

Q

M

A

L

V

S

R

D

D

L

L

K

E

E

A

A

R

L

I

V

R

R

R

I

I

S

L

R

R

binding beta-D-fructofuranose: R47 (≠ Q71), M66 (≠ Q90), F114 (= F138), D116 (= D140), E228 (= E251), W246 (= W269), Y248 (= Y271), W285 (≠ Q308), R321 (≠ S344)
binding alpha-D-glucopyranose: T44 (≠ A68), D68 (= D92), D116 (= D140), Y171 (= Y194), G173 (= G196), C176 (≠ V199), E228 (= E251), W246 (= W269), G283 (= G306), G284 (= G307), W285 (≠ Q308), R354 (= R377)

Sites not aligning to the query:

binding beta-D-fructofuranose: 9

6jadA Crystal structure of abc transporter alpha-glycoside-binding protein in complex with palatinose (see paper)
45% identity, 89% coverage: 44:423/427 of query aligns to 20:400/404 of 6jadA

query
sites
6jadA

C

M

R

K

D

E

G

M

A

V

Q

E

E

A

W

W

A

G

R

K

S

T

G

G

N

T

E

R

V

V

R

E

F

Y

V

I

S

D

P

S

P

P

K

A

G

D

A
x
T

S

N

E

D

Q
x
R

L

L

A

A

L

L

Y

Y

Q

Q

L

Y

L

W

A

A

G

R

S

S

P

P

D

D

I

V

D

D

V

V

F

Y

Q

M

I

I

D
|
D

V

V

V

I

W

W

P

P

G

G

I

I

L

V

G

A

N

P

Y

H

F

A

I

L

D

D

L

L

K

K

D

P

R

Y

A

L

G

T

P

E

D

A

T

E

V

L

G

K

R

E

H

F

L

F

P

P

A

R

M

I

I

V

E

Q

A

N

T

T

V

I

K

R

G

G

R

K

L

L

V

T

A

S

M

L

P

P

W

F

F
|
F

A

T

D
|
D

A

A

G

G

V

I

L

L

Y

Y

A

Y

R

R

K

K

D

D

L

L

E

E

A

K

H

Y

G

G

R

Y

P

T

V

S

P

P

-

P

Q

R

T

T

W

W

E

N

E

E

L

L

Q

E

D

Q

T

M

A

A

A

E

L

R

I

V

Q

M

R

E

A

G

E

E

R

R

A

R

A

A

G

G

R

N

D

R

R

D

M

F

W

W

G

G

Y

F

V

V

W

F

Q

Q

G

G

R

K

A

P

Y
|
Y

E
|
E

G
|
G

L

L

T

T

V
x
C

N

D

A

A

L

L

E

E

W

W

I

I

A

Y

S

S

R

H

N

G

G

G

T

R

I

I

V

V

A

E

P

P

D

D

G

G

A

T

I

I

T

S

I

V

D

N

N

N

P

G

Q

R

A

A

E

L

A

A

L

L

A

N

M

R

A

A

R

H

G

G

W

W

V

V

G

G

S

R

I

I

S

A

P

P

P

Q

G

G

V

V

L

T

N

S

Y

Y

M

A

E
|
E

E

E

A

A

R

R

G

N

V

V

F

W

Q

Q

S

Q

G

G

N

N

A

S

V

L

F

F

M

M

R

R

N

N

W
|
W

P

P

Y
|
Y

A

A

W

Y

T

A

L

L

V

G

N

Q

A

A

A

E

D

G

S

S

A

P

V

I

G

R

G

G

K

K

V

F

A

G

V

V

V

T

P

V

L

L

P

P

K

K

G

A

G

S

P

A

E

D

G

A

R

P

H

N

T

A

S

A

T

T

L

L

G
|
G

Q
x
W

L

Q

L

L

A

M

V

V

S

S

K

A

F

Y

S

S

A

R

H

Y

A

P

D

K

E

E

A

A

A

V

D

D

L

L

A

V

L

K

Y

Y

L

L

T

A

G

S

L

Y

A

E

E

V

Q

Q

K

K

R

D

R

N

A

A

I

V

H

R

G

L

A

S

S
x
R

N

L

P

P

T

T

I

R

P

P

A

A

L

L

Y

Y

E

T

D

D

A

R

E

D

V

V

V

L

A

A

A

R

N

N

P

P

F

W

F

F

A

R

A

D

L

L

A

L

E

P

S

V

I

F

A

Q

N

N

A

A

V

V

N

S

R
|
R

P

P

A

S

Q

D

A

V

T

A

G

G

M

A

R

R

Y

Y

N

N

Q

Q

V

V

S

S

A

E

E

A

F

I

Y

W

A

T

N

E

V

V

H

H

E

S

V

V

L

L

S

T

G

G

R

R

Q

K

D

K

A

G

K

E

A

Q

M

A

L

V

S

R

D

D

L

L

K

E

E

A

A

R

L

I

V

R

R

R

I

I

S

L

R

R

binding alpha-D-glucopyranose: T44 (≠ A68), D68 (= D92), D116 (= D140), Y171 (= Y194), E172 (= E195), G173 (= G196), C176 (≠ V199), E228 (= E251), W246 (= W269), G283 (= G306), G284 (= G307), W285 (≠ Q308), R354 (= R377)
binding alpha-D-fructofuranose: R47 (≠ Q71), F114 (= F138), D116 (= D140), W246 (= W269), Y248 (= Y271), W285 (≠ Q308), R321 (≠ S344)

Sites not aligning to the query:

binding alpha-D-fructofuranose: 9

6j9yA Crystal structure of abc transporter alpha-glycoside-binding protein in complex with maltose (see paper)
45% identity, 89% coverage: 44:423/427 of query aligns to 20:400/404 of 6j9yA

query
sites
6j9yA

C

M

R

K

D

E

G

M

A

V

Q

E

E

A

W

W

A

G

R

K

S

T

G

G

N

T

E

R

V

V

R

E

F

Y

V

I

S

D

P

S

P

P

K
x
A

G

D

A
x
T

S

N

E

D

Q
x
R

L

L

A

A

L

L

Y

Y

Q

Q

L

Y

L

W

A

A

G

R

S

S

P

P

D

D

I

V

D

D

V

V

F

Y

Q

M

I

I

D
|
D

V

V

V

I

W

W

P

P

G

G

I

I

L

V

G

A

N

P

Y

H

F

A

I

L

D

D

L

L

K

K

D

P

R

Y

A

L

G

T

P

E

D

A

T

E

V

L

G

K

R

E

H

F

L

F

P

P

A

R

M

I

I

V

E

Q

A

N

T

T

V

I

K

R

G

G

R

K

L

L

V

T

A

S

M

L

P

P

W

F

F
|
F

A

T

D
|
D

A

A

G

G

V

I

L

L

Y

Y

A

Y

R

R

K

K

D

D

L

L

E

E

A

K

H

Y

G

G

R

Y

P

T

V

S

P

P

-

P

Q

R

T

T

W

W

E

N

E

E

L

L

Q

E

D

Q

T

M

A

A

A

E

L

R

I

V

Q

M

R

E

A

G

E

E

R

R

A

R

A

A

G

G

R

N

D

R

R

D

M

F

W

W

G

G

Y

F

V

V

W

F

Q

Q

G

G

R

K

A

P

Y
|
Y

E

E

G
|
G

L

L

T

T

V
x
C

N

D

A

A

L

L

E

E

W

W

I

I

A

Y

S

S

R

H

N

G

G

G

T

R

I

I

V

V

A

E

P

P

D

D

G

G

A

T

I

I

T

S

I

V

D

N

N

N

P

G

Q

R

A

A

E

L

A

A

L

L

A

N

M

R

A

A

R

H

G

G

W

W

V

V

G

G

S

R

I

I

S

A

P

P

P

Q

G

G

V

V

L

T

N

S

Y

Y

M

A

E
|
E

E

E

A

A

R

R

G

N

V

V

F

W

Q

Q

S

Q

G

G

N

N

A

S

V

L

F

F

M

M

R

R

N

N

W
|
W

P

P

Y
|
Y

A

A

W

Y

T

A

L

L

V

G

N

Q

A

A

A

E

D

G

S

S

A

P

V

I

G

R

G

G

K

K

V

F

A

G

V

V

V

T

P

V

L

L

P

P

K

K

G

A

G

S

P

A

E

D

G

A

R

P

H

N

T

A

S

A

T

T

L

L

G
|
G

Q
x
W

L

Q

L

L

A

M

V

V

S

S

K

A

F

Y

S

S

A

R

H

Y

A

P

D

K

E

E

A

A

A

V

D

D

L

L

A

V

L

K

Y

Y

L

L

T

A

G

S

L

Y

A

E

E

V

Q

Q

K

K

R

D

R

N

A

A

I

V

H

R

G

L

A

S

S
x
R

N

L

P

P

T

T

I

R

P

P

A

A

L

L

Y

Y

E

T

D

D

A

R

E

D

V

V

V

L

A

A

A

R

N

N

P

P

F

W

F

F

A

R

A

D

L

L

A

L

E

P

S

V

I

F

A

Q

N

N

A

A

V

V

N

S

R
|
R

P

P

A

S

Q

D

A

V

T

A

G

G

M

A

R

R

Y

Y

N

N

Q

Q

V

V

S

S

A

E

E

A

F

I

Y

W

A

T

N
x
E

V

V

H

H

E

S

V

V

L

L

S

T

G

G

R

R

Q
x
K

D

K

A

G

K

E

A

Q

M

A

L

V

S

R

D
|
D

L

L

K

E

E

A

A
x
R

L

I

V

R

R

R

I

I

S

L

R

R

binding carbonate ion: E373 (≠ N396), K382 (≠ Q405), D390 (= D413), R394 (≠ A417)
binding alpha-D-glucopyranose: A42 (≠ K66), T44 (≠ A68), R47 (≠ Q71), D68 (= D92), F114 (= F138), D116 (= D140), D116 (= D140), Y171 (= Y194), G173 (= G196), C176 (≠ V199), E228 (= E251), E228 (= E251), W246 (= W269), W246 (= W269), Y248 (= Y271), G283 (= G306), G284 (= G307), W285 (≠ Q308), W285 (≠ Q308), R321 (≠ S344), R354 (= R377)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 9, 13

6jamA Crystal structure of abc transporter alpha-glycoside-binding mutant protein r356a in complex with trehalose (see paper)
45% identity, 89% coverage: 44:423/427 of query aligns to 22:402/406 of 6jamA

query
sites
6jamA

C

M

R

K

D

E

G

M

A

V

Q

E

E

A

W

W

A

G

R

K

S

T

G

G

N

T

E

R

V

V

R

E

F

Y

V

I

S

D

P

S

P

P

K

A

G

D

A
x
T

S

N

E

D

Q
x
R

L

L

A

A

L

L

Y

Y

Q

Q

L

Y

L

W

A

A

G

R

S

S

P

P

D

D

I

V

D

D

V

V

F

Y

Q

M

I

I

D
|
D

V

V

V

I

W

W

P

P

G

G

I

I

L

V

G

A

N

P

Y

H

F

A

I

L

D

D

L

L

K

K

D

P

R

Y

A

L

G

T

P

E

D

A

T

E

V

L

G

K

R

E

H

F

L

F

P

P

A

R

M

I

I

V

E

Q

A

N

T

T

V

I

K

R

G

G

R

K

L

L

V

T

A

S

M

L

P

P

W

F

F
|
F

A

T

D
|
D

A

A

G

G

V

I

L

L

Y

Y

A

Y

R

R

K

K

D

D

L

L

E

E

A

K

H

Y

G

G

R

Y

P

T

V

S

P

P

-

P

Q

R

T

T

W

W

E

N

E

E

L

L

Q

E

D

Q

T

M

A

A

A

E

L

R

I

V

Q

M

R

E

A

G

E

E

R

R

A

R

A

A

G

G

R

N

D

R

R

D

M

F

W

W

G

G

Y

F

V

V

W

F

Q

Q

G

G

R

K

A

P

Y
|
Y

E
|
E

G
|
G

L

L

T

T

V
x
C

N

D

A

A

L

L

E

E

W

W

I

I

A

Y

S

S

R

H

N

G

G

G

T

R

I

I

V

V

A

E

P

P

D

D

G

G

A

T

I

I

T

S

I

V

D

N

N

N

P

G

Q

R

A

A

E

L

A

A

L

L

A

N

M

R

A

A

R

H

G

G

W

W

V

V

G

G

S

R

I

I

S

A

P

P

P

Q

G

G

V

V

L

T

N

S

Y

Y

M

A

E
|
E

E

E

A

A

R

R

G

N

V

V

F

W

Q

Q

S

Q

G

G

N

N

A

S

V

L

F

F

M

M

R

R

N

N

W
|
W

P

P

Y
|
Y

A

A

W

Y

T

A

L

L

V

G

N

Q

A

A

A

E

D

G

S

S

A

P

V

I

G

R

G

G

K

K

V

F

A

G

V

V

V

T

P

V

L

L

P

P

K

K

G

A

G

S

P

A

E

D

G

A

R

P

H

N

T

A

S

A

T

T

L

L

G
|
G

Q
x
W

L

Q

L

L

A

M

V

V

S

S

K

A

F

Y

S

S

A

R

H

Y

A

P

D

K

E

E

A

A

A

V

D

D

L

L

A

V

L

K

Y

Y

L

L

T

A

G

S

L

Y

A

E

E

V

Q

Q

K

K

R

D

R

N

A

A

I

V

H

R

G

L

A

S

S
x
R

N

L

P

P

T

T

I

R

P

P

A

A

L

L

Y

Y

E

T

D

D

A

R

E

D

V

V

V

L

A

A

A

R

N

N

P

P

F

W

F

F

A

R

A

D

L

L

A

L

E

P

S

V

I

F

A

Q

N

N

A

A

V

V

N

S

R

A

P

P

A

S

Q

D

A

V

T

A

G

G

M

A

R

R

Y

Y

N

N

Q

Q

V

V

S

S

A

E

E

A

F

I

Y

W

A

T

N

E

V

V

H

H

E

S

V

V

L

L

S

T

G

G

R

R

Q

K

D

K

A

G

K

E

A

Q

M

A

L

V

S

R

D

D

L

L

K

E

E

A

A

R

L

I

V

R

R

R

I

I

S

L

R

R

binding alpha-D-glucopyranose: T46 (≠ A68), R49 (≠ Q71), D70 (= D92), F116 (= F138), D118 (= D140), D118 (= D140), Y173 (= Y194), E174 (= E195), G175 (= G196), C178 (≠ V199), E230 (= E251), W248 (= W269), W248 (= W269), Y250 (= Y271), G285 (= G306), G286 (= G307), W287 (≠ Q308), W287 (≠ Q308), R323 (≠ S344)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 11

6jaiA Crystal structure of abc transporter alpha-glycoside-binding mutant protein d118a in complex with maltose (see paper)
45% identity, 89% coverage: 44:423/427 of query aligns to 20:400/404 of 6jaiA

query
sites
6jaiA

C

M

R

K

D

E

G

M

A

V

Q

E

E

A

W

W

A

G

R

K

S

T

G

G

N

T

E

R

V

V

R

E

F

Y

V

I

S

D

P

S

P

P

K
x
A

G

D

A
x
T

S

N

E

D

Q

R

L

L

A

A

L

L

Y

Y

Q

Q

L

Y

L

W

A

A

G

R

S

S

P

P

D

D

I

V

D

D

V

V

F

Y

Q

M

I

I

D
|
D

V

V

V

I

W

W

P

P

G

G

I

I

L

V

G

A

N

P

Y

H

F

A

I

L

D

D

L

L

K

K

D

P

R

Y

A

L

G

T

P

E

D

A

T

E

V

L

G

K

R

E

H

F

L

F

P

P

A

R

M

I

I

V

E

Q

A

N

T

T

V

I

K

R

G

G

R

K

L

L

V

T

A

S

M

L

P

P

W

F

F
|
F

A

T

D

A

A

A

G

G

V

I

L

L

Y

Y

A

Y

R

R

K

K

D

D

L

L

E

E

A

K

H

Y

G

G

R

Y

P

T

V

S

P

P

-

P

Q

R

T

T

W

W

E

N

E

E

L

L

Q

E

D

Q

T

M

A

A

A

E

L

R

I

V

Q

M

R

E

A

G

E

E

R

R

A

R

A

A

G

G

R

N

D

R

R

D

M

F

W

W

G

G

Y

F

V

V

W

F

Q

Q

G

G

R

K

A

P

Y
|
Y

E
|
E

G
|
G

L

L

T

T

V
x
C

N

D

A

A

L

L

E

E

W

W

I

I

A

Y

S

S

R

H

N

G

G

G

T

R

I

I

V

V

A

E

P

P

D

D

G

G

A

T

I

I

T

S

I

V

D

N

N

N

P

G

Q

R

A

A

E

L

A

A

L

L

A

N

M

R

A

A

R

H

G

G

W

W

V

V

G

G

S

R

I

I

S

A

P

P

P

Q

G

G

V

V

L

T

N

S

Y

Y

M

A

E
|
E

E

E

A

A

R

R

G

N

V

V

F

W

Q

Q

S

Q

G

G

N

N

A

S

V

L

F

F

M

M

R

R

N

N

W
|
W

P
|
P

Y
|
Y

A

A

W

Y

T

A

L

L

V

G

N

Q

A

A

A

E

D

G

S

S

A

P

V

I

G

R

G

G

K

K

V

F

A

G

V

V

V

T

P

V

L

L

P

P

K

K

G

A

G

S

P

A

E

D

G

A

R

P

H

N

T

A

S

A

T

T

L

L

G
|
G

Q
x
W

L

Q

L

L

A

M

V

V

S

S

K

A

F

Y

S

S

A

R

H

Y

A

P

D

K

E

E

A

A

A

V

D

D

L

L

A

V

L

K

Y

Y

L

L

T

A

G

S

L

Y

A

E

E

V

Q

Q

K

K

R

D

R

N

A

A

I

V

H

R

G

L

A

S

S
x
R

N

L

P

P

T

T

I

R

P

P

A

A

L

L

Y

Y

E

T

D

D

A

R

E

D

V

V

V

L

A

A

A

R

N

N

P

P

F

W

F

F

A

R

A

D

L

L

A

L

E

P

S

V

I

F

A

Q

N

N

A

A

V

V

N

S

R
|
R

P

P

A

S

Q

D

A

V

T

A

G

G

M

A

R

R

Y

Y

N

N

Q

Q

V

V

S

S

A

E

E

A

F

I

Y

W

A

T

N

E

V

V

H

H

E

S

V

V

L

L

S

T

G

G

R

R

Q

K

D

K

A

G

K

E

A

Q

M

A

L

V

S

R

D

D

L

L

K

E

E

A

A

R

L

I

V

R

R

R

I

I

S

L

R

R

binding calcium ion: P247 (= P270), Y248 (= Y271), R321 (≠ S344)
binding alpha-D-glucopyranose: A42 (≠ K66), T44 (≠ A68), D68 (= D92), F114 (= F138), Y171 (= Y194), E172 (= E195), G173 (= G196), C176 (≠ V199), E228 (= E251), E228 (= E251), W246 (= W269), W246 (= W269), Y248 (= Y271), G283 (= G306), G284 (= G307), W285 (≠ Q308), W285 (≠ Q308), R321 (≠ S344), R354 (= R377)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 9, 13

6dtqA Maltose bound t. Maritima male3 (see paper)
38% identity, 91% coverage: 30:418/427 of query aligns to 4:387/391 of 6dtqA

query
sites
6dtqA

V

I

T

T

L

M

A

T

C

S

S

G

G

G

L
x
V

G

G

I

K

S
x
E

F

L

D

E

L

V

C

L

R

K

D

K

G

Q

A

L

Q

E

E

M

W

F

A

H

R

Q

S

Y

G

P

N

D

-

I

E

E

V

V

R

E

F

I

V

I

S

P

P

M

P

P

K
x
D

G

S

A
x
S

S

T

E

E

Q
x
R

L

H

A

D

L

L

Y

Y

Q

V

Q

T

L

Y

L

F

A

A

G

G

S

E

P
x
T

D
|
D

I

P

D
|
D

V

V

F

L

Q

M

I

L

D
|
D

V

V

V

I

W

W

P

P

G

A

I

E

L

F

G

A

N

P

Y

F

F

L

I

E

D

D

L

L

K

T

D

A

R

D

A

K

G

D

P

Y

D

F

T

E

V

L

G

G

R

E

H

F

L

L

P

P

A

G

M

T

I

V

E

M

A

S

A

V

T

T

V

V

K

N

G

G

R

R

L

I

V

V

A

A

M

V

P

P

W

W

F
|
F

A

T

D
|
D

A

A

G

G

V

L

L

L

Y

Y

A

Y

R

R

K

K

D

D

L

L

E

E

A

K

H

Y

G

G

R

Y

P

D

-

H

V

A

P

P

Q

R

T

T

W

W

E

D

E

E

L

L

Q

V

D

E

T

M

A

A

A

K

L

K

I

I

Q

S

R

Q

A

A

E

E

R

-

A

-

G

-

R

-

D

-

R

G

M

I

W

H

G

G

Y

F

V

V

W

W

Q

Q

G

G

R

A

A

R

Y
|
Y

E
|
E

G
|
G

L

L

T

V

V
x
C

N

D

A

F

L

L

E

E

W

Y

I

L

A

W

S

S

R

F

N

-

G

G

T

D

I

V

V

L

A

D

P

E

D

S

G

G

A

K

I

V

T

V

I

I

D

D

N

S

P

P

Q

E

A

A

A

V

E

A

A

A

L

L

A

Q

M

F

A

M

R

V

G

D

W

L

V

I

-

Y

-

K

G

H

S

K

I

V

S

T

P

P

P

E

G

G

V

V

L

T

N

T

Y

Y

M

M

E
|
E

E

E

E

D

A

A

R

R

G

R

V

I

F

F

Q

Q

S

N

G

G

N

E

A

A

V

V

F

F

M

M

R

R

N

N

W
|
W

P

P

Y
|
Y

A

A

W

W

T

S

L

L

V

V

N

N

A

S

A

D

D

E

S

S

A

P

V

I

G

K

G

G

K

K

V

V

A

G

V

V

V

A

P

P

L

L

P

P

K

M

G

-

G

G

P

P

E

G

G

G

R

R

H

R

T

A

S

A

T

T

L

L

G
|
G

Q
x
W

L

V

L

L

A

G

V

I

S

N

K

K

F

F

S

S

A

S

-

P

-

E

H
x
E

A

K

D

E

E

A

A

A

A

K

D

K

L

L

A

I

L

K

Y

F

L

L

T

T

G

S

L

Y

A

D

E

Q

Q

Q

K

L

R

Y

R

K

A

A

I

I

H

N

G

A

A

G

S
x
Q

N

N

P

P

T

T

I

R

P

K

A

A

L

V

Y

Y

E

K

D

D

A

P

E

K

V

L

V

K

A

E

A

A

N

A

P

P

F

F

F

M

A

V

A

E

L

L

A

L

E

G

S

V

I

F

A

I

N

N

A

A

V

L

N

P

R
|
R

P

P

A

R

Q

V

A

A

T

N

G

-

M

-

R

-

Y

Y

N

T

Q

E

V

V

S

S

A

D

E

V

F

I

Y

Q

A

R

N

Y

V

V

H

H

E

A

V

A

L

L

S

T

G

R

R

Q

Q

T

D

T

A

S

K

E

A

D

M

A

L

I

S

K

D

N

L

I

K

A

E

K

A

E

L

L

binding alpha-D-glucopyranose: V11 (≠ L37), E14 (≠ S40), D41 (≠ K66), S43 (≠ A68), R46 (≠ Q71), D67 (= D92), F113 (= F138), D115 (= D140), D115 (= D140), Y164 (= Y194), E165 (= E195), G166 (= G196), C169 (≠ V199), E222 (= E251), W240 (= W269), W240 (= W269), Y242 (= Y271), G276 (= G306), G277 (= G307), W278 (≠ Q308), W278 (≠ Q308), Q316 (≠ S344), R349 (= R377)
binding magnesium ion: T59 (≠ P84), D60 (= D85), D62 (= D87), E290 (≠ H318)

1eu8A Structure of trehalose maltose binding protein from thermococcus litoralis (see paper)
38% identity, 76% coverage: 79:404/427 of query aligns to 56:387/407 of 1eu8A

query
sites
1eu8A

L

L

A

R

A

G

G

K

S

S

P

S

D

D

I

P

D

D

V

V

F

F

Q

L

I

M

D
|
D

V

V

V

A

W

W

P

L

G

G

-

Q

-

F

I

I

L

A

G

S

N

G

Y

W

F

L

I

E

D

P

L

L

K

D

D

D

R

Y

A

V

G

Q

P

K

D

D

T

N

-

Y

-

D

V

L

G

S

R

V

H

F

L

F

P

Q

A

S

M

V

I

I

E

N

A

L

A

A

T

D

V

K

K

Q

G

G

-

G

R

K

L

L

V

Y

A

A

M

L

P

P

W

V

F
x
Y

A

I

D
|
D

A

A

G

G

V

L

L

L

Y

Y

A

Y

R

R

K

K

D

D

L

L

E

E

A

K

H

Y

G

G

-

Y

-

S

R

K

P

P

V

-

P

P

Q

E

T

T

W

W

E

Q

E

E

L

L

Q

V

D

E

T

M

A

A

A

Q

L

K

I

I

Q

Q

R

S

A

G

E

E

R

R

A

E

A

T

G

N

R

P

D

N

R

-

M

F

W

W

G

G

Y

F

V

V

W

W

Q

Q

G

G

R

K

A

Q

Y
|
Y

E
|
E

G
|
G

L

L

T

V

V
x
C

N

D

A

F

L

V

E

E

W

Y

I

V

A

Y

S

S

R

N

N

G

G

G

G

S

T

L

I

G

V

E

A

F

P

K

D

D

G

G

-

K

-

W

A

V

I

P

T

T

I

L

D

N

N

K

P

P

Q

E

A

N

A

V

E

E

A

A

L

L

A

Q

M

F

A

M

R

V

G

D

W

L

V

I

G

H

S

K

-

Y

-

K

I

I

S

S

P

P

G

N

V

T

L

Y

N

T

Y

E

M

M

E

T

E
|
E

E

E

-

P

A

V

R

R

G

L

V

M

F

F

Q

Q

S

Q

G

G

N

N

A

A

V

A

F

F

M

E

R

R

N

N

W
|
W

P

P

Y
|
Y

A

A

W

W

T

G

L

L

V

H

N

N

A

A

A

D

D

D

S

S

A

P

V

V

G

K

G

G

K

K

V

V

A

G

V

V

V

A

P

P

L

L

P

P

K

H

G

-

G

F

P

P

E

G

G

H

R

K

H

S

T

A

S

A

T

T

L

L

G
|
G

Q
x
W

L

H

L

I

A

G

V

I

S

S

K

K

F

Y

S

S

A

D

H

N

A

K

D

A

E

L

A

A

A

W

D

E

L

F

A

V

L

K

Y

F

L

V

T

E

G

S

L

Y

A

S

E

V

Q

Q

K

K

R

G

R

F

A

A

I

M

H

N

G

L

A

G

S

W

N

N

P

P

T

G

I

R

P

V

A

D

L

V

Y

Y

E

D

D

D

A

P

E

A

V

V

A

S

A

K

N

S

P

P

F

H

F

L

A

K

A

E

L

L

A

R

E

A

S

V

I

F

A

E

N

N

A

A

V

V

N

P

R
|
R

P

P

A

I

Q

V

A

P

T

Y

G

-

M

-

R

-

Y

Y

N

P

Q

Q

V

L

S

S

A

E

E

I

F

I

Y

Q

A

K

N

Y

V

V

H

N

E

S

V

A

L

L

S

A

G

G

R

K

binding alpha-D-glucopyranose: D69 (= D92), Y120 (≠ F138), D122 (= D140), D122 (= D140), Y176 (= Y194), E177 (= E195), G178 (= G196), C181 (≠ V199), E238 (= E252), W256 (= W269), Y258 (= Y271), G292 (= G306), G293 (= G307), W294 (≠ Q308), W294 (≠ Q308), R363 (= R377)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 12, 16, 43, 45, 45, 48

Q7LYW7 Trehalose/maltose-binding protein MalE; TMBP from Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C) (see paper)
38% identity, 76% coverage: 79:404/427 of query aligns to 97:428/450 of Q7LYW7

query
sites
Q7LYW7

L

L

A

R

A

G

G

K

S

S

P

S

D

D

I

P

D

D

V

V

F

F

Q

L

I

M

D
|
D

V

V

V

A

W

W

P

L

G

G

-

Q

-

F

I

I

L

A

G

S

N

G

Y

W

F

L

I

E

D

P

L

L

K

D

D

D

R

Y

A

V

G

Q

P

K

D

D

T

N

-

Y

-

D

V

L

G

S

R

V

H

F

L

F

P

Q

A

S

M

V

I

I

E

N

A

L

A

A

T

D

V

K

K

Q

G

G

-

G

R

K

L

L

V

Y

A

A

M

L

P

P

W

V

F
x
Y

A

I

D
|
D

A

A

G

G

V

L

L

L

Y

Y

A

Y

R

R

K

K

D

D

L

L

E

E

A

K

H

Y

G

G

-

Y

-

S

R

K

P

P

V

-

P

P

Q

E

T

T

W

W

E

Q

E

E

L

L

Q

V

D

E

T

M

A

A

A

Q

L

K

I

I

Q

Q

R

S

A

G

E

E

R

R

A

E

A

T

G

N

R

P

D

N

R

-

M

F

W

W

G

G

Y

F

V

V

W

W

Q

Q

G

G

R

K

A

Q

Y
|
Y

E

E

G

S

L

L

T

V

V

C

N

D

A

F

L

V

E

E

W

Y

I

V

A

Y

S

S

R

N

N

G

G

G

G

S

T

L

I

G

V

E

A

F

P

K

D

D

G

G

-

K

-

W

A

V

I

P

T

T

I

L

D

N

N

K

P

P

Q

E

A

N

A

V

E

E

A

A

L

L

A

Q

M

F

A

M

R

V

G

D

W

L

V

I

G

H

S

K

-

Y

-

K

I

I

S

S

P

P

G

N

V

T

L

Y

N

T

Y

E

M

M

E

T

E
|
E

E

E

-

P

A

V

R

R

G

L

V

M

F

F

Q

Q

S

Q

G

G

N

N

A

A

V

A

F

F

M

E

R

R

N

N

W
|
W

P

P

Y
|
Y

A

A

W

W

T

G

L

L

V

H

N

N

A

A

A

D

D

D

S

S

A

P

V

V

G

K

G

G

K

K

V

V

A

G

V

V

V

A

P

P

L

L

P

P

K

H

G

-

G

F

P

P

E

G

G

H

R

K

H

S

T

A

S

A

T

T

L

L

G

G

G
|
G

Q
x
W

L

H

L

I

A

G

V

I

S

S

K

K

F

Y

S

S

A

D

H

N

A

K

D

A

E

L

A

A

A

W

D

E

L

F

A

V

L

K

Y

F

L

V

T

E

G

S

L

Y

A

S

E

V

Q

Q

K

K

R

G

R

F

A

A

I

M

H

N

G

L

A

G

S
x
W

N

N

P

P

T

G

I

R

P

V

A

D

L

V

Y

Y

E

D

D

D

A

P

E

A

V

V

A

S

A

K

N

S

P

P

F

H

F

L

A

K

A

E

L

L

A

R

E

A

S

V

I

F

A

E

N

N

A

A

V

V

N

P

R
|
R

P

P

A

I

Q

V

A

P

T

-

G

-

M

-

R

Y

Y

Y

N

P

Q

Q

V

L

S

S

A

E

E

I

F

I

Y

Q

A

K

N

Y

V

V

H

N

E

S

V

A

L

L

S

A

G

G

R

K

D110 (= D92) binding
Y161 (≠ F138) binding
D163 (= D140) binding
Y217 (= Y194) binding
E279 (= E252) binding
W297 (= W269) binding
Y299 (= Y271) binding
G334 (= G307) binding
W335 (≠ Q308) binding
W371 (≠ S344) binding
R404 (= R377) binding

Sites not aligning to the query:

57 binding
84 binding
89 binding

7ofyA Crystal structure of sq binding protein from agrobacterium tumefaciens in complex with sulfoquinovosyl glycerol (sqgro) (see paper)
34% identity, 82% coverage: 67:418/427 of query aligns to 41:387/389 of 7ofyA

query
sites
7ofyA

G

N

A
x
T

S
|
S

E

E

Q

M

L

Q

A

A

L

Q

Y

Y

-

L

Q

N

Q

T

L

V

L

M

A

S

A

A

G

K

S

D

P

S

D

S

I

L

D

D

V

V

F

L

Q

M

I

L

D
|
D

V

V

V

I

W

R

P

P

G

A

I

Q

L

F

G

A

N

T

Y

A

F

G

I

W

D

-

L

T

K

S

D

D

R

F

A

S

G

G

P

K

D

D

T

-

V

L

G

S

R

A

H

Y

L

L

P

P

A

T

M

Y

I

A

E

E

A

A

A

N

T

T

V

V

K

N

G

G

R

K

L

I

V

V

A

A

M

L

P

P

W

A

F
|
F

A

A

D
|
D

A

S

G

M

V

F

L

L

Y

Y

A

Y

R

R

K

K

D

D

L

L

E

D

A

K

H

Y

G

G

R

I

P

K

V

P

P

P

Q

T

T

T

W

W

E

D

E

E

L

L

Q

K

D

E

T

A

A

S

A

K

L

K

I

V

Q

M

R

E

A

G

E

E

R

K

A

-

G

-

R

N

D

P

R

E

M

L

W

Q

G

G

Y

L

V

S

W

F

Q

Q

G

G

R

K

A

A

Y
x
I

E
|
E

G
|
G

L

A

T

V

V

C

N

T

A

F

L

L

E

-

W

-

I

L

A

P

S

Y

R

W

N

S

G

E

G

G

T

K

I

S

V

L

A

V

P

E

D

N

G

G

A

K

I

L

T

N

I

F

D

D

N

N

P

K

Q

A

A

A

A

V

E

D

A

S

L

L

A

K

M

L

A

W

R

K

G

S

W

F

V

V

G

D

S

D

-

G

I

I

S

S

P

K

G

N

V

I

L

S

N

E

Y

V

M

A

E
x
T

E

D

A

T

R

R

G

K

V

E

F

F

Q

Q

S

A

G

G

N

K

A

V

V

L

F

F

M

A

R

V

N

N

W
|
W

P

S

Y
|
Y

A

A

W

W

T

T

L

H

V

F

N

Q

A

G

A

K

D

E

S

S

A

Q

V

V

G

N

G

D

K

K

V

V

A

G

V

V

V

A

P

R

L

L

P

P

-

A

-

V

K

K

G

G

G

-

P

-

E

-

G

G

R

E

H

Q

T

T

S

T

T

C

L

L

G
|
G

Q
x
W

L

E

L

F

A

G

V

V

S

S

K

A

F

Y

S

S

A

K

H

Q

A

Q

D

D

E

E

A

A

A

K

D

K

L

L

A

V

L

E

Y

Y

L

L

T

S

G

S

L

Q

A

D

E

V

Q

S

K

K

R

F

R

M

A

A

I

I

H

N

G

A

A

A

S

L

N

L

P

P

T

T

I

Y

P

A

A

A

L

L

Y

Y

E

K

D

D

A

A

E

D

V

V

V

T

A

K

A

T

N

I

P

P

F

W

F

F

A

A

A

D

L

A

A

L

E

P

S

V

I

V

A

E

N

T

A

A

V

K

N

A

R
|
R

P

P

A

V

Q

T

A

P

T

-

G

-

M

-

R

R

Y

Y

N

N

Q

E

V

V

S

S

A

E

E

T

F

I

Y

R

A

T

N

T

V

V

H

N

E

G

V

V

L

L

S

A

G

G

-

V

-

M

-

T

-

P

R

E

Q

D

D

G

A

A

K

K

A

Q

M

M

L

E

S

S

D

R

L

L

K

R

E

R

A

V

L

L

binding [(2S,3S,4S,5R,6S)-6-[(2R)-2,3-bis(oxidanyl)propoxy]-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: T42 (≠ A68), S43 (= S69), D67 (= D92), F111 (= F138), D113 (= D140), I164 (≠ Y194), E165 (= E195), G166 (= G196), T220 (≠ E251), W238 (= W269), Y240 (= Y271), G274 (= G306), G275 (= G307), W276 (≠ Q308), R345 (= R377)

Sites not aligning to the query:

binding [(2S,3S,4S,5R,6S)-6-[(2R)-2,3-bis(oxidanyl)propoxy]-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: 12, 13

7yzsAAA Sulfoquinovosyl binding protein (see paper)
34% identity, 82% coverage: 67:415/427 of query aligns to 39:382/384 of 7yzsAAA

query
sites
7yzsAAA

G

N

A
x
T

S
|
S

E

E

Q

M

L

Q

A

A

L

Q

Y

Y

-

L

Q

N

Q

T

L

V

L

M

A

S

A

A

G

K

S

D

P

S

D

S

I

L

D

D

V

V

F

L

Q

M

I

L

D
|
D

V

V

V

I

W

R

P

P

G

A

I

Q

L

F

G

A

N

T

Y

A

F

G

I

W

D

-

L

T

K

S

D

D

R

F

A

S

G

G

P

K

D

D

T

-

V

L

G

S

R

A

H

Y

L

L

P

P

A

T

M

Y

I

A

E

E

A

A

A

N

T

T

V

V

K

N

G

G

R

K

L

I

V

V

A

A

M

L

P

P

W

A

F

F

A

A

D
|
D

A

S

G

M

V

F

L

L

Y

Y

A

Y

R

R

K

K

D

D

L

L

E

D

A

K

H

Y

G

G

R

I

P

K

V

P

P

P

Q

T

T

T

W

W

E

D

E

E

L

L

Q

K

D

E

T

A

A

S

A

K

L

K

I

V

Q

M

R

E

A

G

E

E

R

K

A

-

G

-

R

N

D

P

R

E

M

L

W

Q

G

G

Y

L

V

S

W

F

Q

Q

G

G

R

K

A

A

Y
x
I

E
|
E

G
|
G

L

A

T

V

V

C

N

T

A

F

L

L

E

-

W

-

I

L

A

P

S

Y

R

W

N

S

G

E

G

G

T

K

I

S

V

L

A

V

P

E

D

N

G

G

A

K

I

L

T

N

I

F

D

D

N

N

P

K

Q

A

A

A

A

V

E

D

A

S

L

L

A

K

M

L

A

W

R

K

G

S

W

F

V

V

G

D

S

D

-

G

I

I

S

S

P

K

G

N

V

I

L

S

N

E

Y

V

M

A

E
x
T

E

D

A

T

R

R

G

K

V

E

F

F

Q

Q

S

A

G

G

N

K

A

V

V

L

F

F

M

A

R

V

N

N

W
|
W</