SitesBLAST
Comparing AZOBR_RS26815 FitnessBrowser__azobra:AZOBR_RS26815 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5y8hA Mycobacterium tuberculosis 3-hydroxyisobutyrate dehydrogenase (mthibadh) + NAD+ (see paper)
52% identity, 98% coverage: 4:295/299 of query aligns to 3:290/291 of 5y8hA
- binding (2~{S})-2-methylpentanedioic acid: R144 (= R148), E148 (≠ T152), A151 (≠ G155), K153 (= K157)
- binding nicotinamide-adenine-dinucleotide: G7 (= G8), G9 (= G10), N10 (= N11), M11 (= M12), F29 (≠ Y30), D30 (= D31), P31 (≠ V32), M63 (= M64), L64 (≠ I65), G120 (≠ A124), L239 (≠ M243), K242 (= K246)
5y8lB Mycobacterium tuberculosis 3-hydroxyisobutyrate dehydrogenase (mthibadh) + NAD +(s)-3-hydroxyisobutyrate (s-hiba) (see paper)
52% identity, 97% coverage: 1:289/299 of query aligns to 1:285/290 of 5y8lB
- binding (2~{S})-2-methylpentanedioic acid: T129 (= T132), E149 (≠ T152), A152 (≠ G155), G153 (≠ P156), G153 (≠ P156), K154 (= K157)
- binding (2S)-2-methyl-3-oxidanyl-propanoic acid: S119 (= S122), G120 (= G123), W211 (= W214), F236 (= F239)
- binding nicotinamide-adenine-dinucleotide: G8 (= G8), G10 (= G10), N11 (= N11), M12 (= M12), F30 (≠ Y30), D31 (= D31), P32 (≠ V32), M64 (= M64), L65 (≠ I65), T93 (= T96), G121 (≠ A124), K168 (= K171), L240 (≠ M243), K243 (= K246)
5y8kA Mycobacterium tuberculosis 3-hydroxyisobutyrate dehydrogenase (mthibadh) + l-serine (see paper)
52% identity, 97% coverage: 1:289/299 of query aligns to 1:285/290 of 5y8kA
5y8iA Mycobacterium tuberculosis 3-hydroxyisobutyrate dehydrogenase (mthibadh) + (s)-3-hydroxyisobutyrate (s-hiba) (see paper)
52% identity, 98% coverage: 4:295/299 of query aligns to 3:290/292 of 5y8iA
P31937 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Homo sapiens (Human) (see paper)
41% identity, 97% coverage: 4:292/299 of query aligns to 42:330/336 of P31937
- LP 103:104 (≠ IP 65:66) binding
- N108 (≠ H70) binding
- T134 (= T96) binding
- K284 (= K246) binding
Sites not aligning to the query:
- 1:36 modified: transit peptide, Mitochondrion
- 40:68 binding
2i9pB Crystal structure of human hydroxyisobutyrate dehydrogenase complexed with NAD+
41% identity, 97% coverage: 4:292/299 of query aligns to 3:291/296 of 2i9pB
- binding nicotinamide-adenine-dinucleotide: G9 (= G10), N10 (= N11), M11 (= M12), Y29 (= Y30), D30 (= D31), V31 (= V32), M63 (= M64), L64 (≠ I65), P65 (= P66), T95 (= T96), V120 (= V121), G122 (= G123), F238 (= F239), K245 (= K246)
P29266 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Rattus norvegicus (Rat) (see paper)
39% identity, 97% coverage: 4:292/299 of query aligns to 41:329/335 of P29266
- D68 (= D31) mutation to R: Decrease of activity with NAD, increase of activity with NADP.
- K208 (= K171) mutation K->A,H,N,R: Complete loss of activity.
- N212 (= N175) mutation to Q: Decrease in activity.
Q9I5I6 NAD-dependent L-serine dehydrogenase; L-serine 3-dehydrogenase (NAD(+)); EC 1.1.1.387 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
47% identity, 98% coverage: 1:292/299 of query aligns to 1:292/298 of Q9I5I6
- 2:31 (vs. 2:31, 57% identical) binding
- P66 (= P66) binding
- T96 (= T96) binding ; mutation to A: Almost abolished activity.
- S122 (= S122) mutation to A: Strongly reduced activity.
- K171 (= K171) active site
- N175 (= N175) mutation to A: Strongly reduced activity.
- W214 (= W214) mutation to A: Almost abolished activity.
- Y219 (= Y219) mutation to A: Strongly reduced activity.
- K246 (= K246) binding ; mutation to A: Almost abolished activity.
- D247 (= D247) mutation to A: Almost abolished activity.
3q3cA Crystal structure of a serine dehydrogenase from pseudomonas aeruginosa pao1 in complex with NAD (see paper)
47% identity, 97% coverage: 3:292/299 of query aligns to 2:290/294 of 3q3cA
- binding nicotinamide-adenine-dinucleotide: G9 (= G10), H10 (≠ N11), M11 (= M12), F29 (≠ Y30), D30 (= D31), L31 (≠ V32), M63 (= M64), L64 (≠ I65), P65 (= P66), T94 (= T96), V119 (= V121), G121 (= G123), F237 (= F239), K244 (= K246)
3obbA Crystal structure of a possible 3-hydroxyisobutyrate dehydrogenase from pseudomonas aeruginosa pao1 (see paper)
46% identity, 98% coverage: 1:292/299 of query aligns to 1:291/295 of 3obbA
5je8B The crystal structure of bacillus cereus 3-hydroxyisobutyrate dehydrogenase in complex with NAD (see paper)
30% identity, 99% coverage: 1:296/299 of query aligns to 3:293/294 of 5je8B
3ws7A The 1.18 a resolution structure of l-serine 3-dehydrogenase complexed with NADP+ and sulfate ion from the hyperthermophilic archaeon pyrobaculum calidifontis (see paper)
36% identity, 94% coverage: 3:282/299 of query aligns to 15:284/293 of 3ws7A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G8), L21 (= L9), G22 (= G10), I23 (≠ N11), M24 (= M12), N43 (≠ D31), R44 (≠ V32), T45 (≠ V33), K48 (≠ A36), M76 (= M64), V77 (≠ I65), S78 (≠ P66), D82 (≠ H70), Q85 (≠ A73), V133 (= V121), F241 (= F239), K242 (≠ A240), H245 (≠ M243), K248 (= K246)
- binding sulfate ion: T134 (≠ S122), G135 (= G123), K183 (= K171)
3w6zA Crystal structure of NADP bound l-serine 3-dehydrogenase (k170m) from hyperthermophilic archaeon pyrobaculum calidifontis (see paper)
35% identity, 94% coverage: 3:282/299 of query aligns to 15:287/296 of 3w6zA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G8), L21 (= L9), G22 (= G10), I23 (≠ N11), M24 (= M12), N43 (≠ D31), R44 (≠ V32), T45 (≠ V33), K48 (≠ A36), V77 (≠ I65), S78 (≠ P66), D82 (≠ H70), Q85 (≠ A73), V133 (= V121), F244 (= F239), K245 (≠ A240), H248 (≠ M243), K251 (= K246)
P0A9V8 3-sulfolactaldehyde reductase; SLA reductase; 4-hydroxybutyrate dehydrogenase; Gamma-hydroxybutyrate dehydrogenase; GHBDH; Succinic semialdehyde reductase; SSA reductase; EC 1.1.1.373; EC 1.1.1.61 from Escherichia coli (strain K12)
33% identity, 97% coverage: 1:289/299 of query aligns to 1:283/298 of P0A9V8
- QM 11:12 (≠ NM 11:12) binding
- D31 (= D31) binding
- L65 (≠ I65) binding
- T96 (= T96) binding
- G122 (≠ S122) mutation to S: 25-fold decrease in catalytic efficiency with SLA as substrate. 5-fold decrease in catalytic efficiency with NADH as substrate.
- R123 (≠ G123) binding ; mutation to G: 130-fold decrease in catalytic efficiency with SLA as substrate. 3-fold decrease in catalytic efficiency with NADH as substrate.
- T124 (≠ A124) mutation to G: 230-fold decrease in catalytic efficiency with SLA as substrate. 12-fold decrease in catalytic efficiency with NADH as substrate.
- NNYMS 174:178 (≠ NNMMA 174:178) binding
- K240 (= K246) binding
6smyA Crystal structure of sla reductase yihu from e. Coli with nadh and product dhps
33% identity, 96% coverage: 2:289/299 of query aligns to 1:282/294 of 6smyA
6smzC Crystal structure of sla reductase yihu from e. Coli in complex with nadh
33% identity, 96% coverage: 2:289/299 of query aligns to 1:282/295 of 6smzC
- binding nicotinamide-adenine-dinucleotide: G9 (= G10), Q10 (≠ N11), M11 (= M12), F29 (≠ Y30), D30 (= D31), V31 (= V32), M63 (= M64), L64 (≠ I65), V73 (≠ A74), S94 (= S95), T95 (= T96), R122 (≠ G123)
3pduA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter sulfurreducens in complex with NADP+ (see paper)
33% identity, 98% coverage: 1:292/299 of query aligns to 1:285/287 of 3pduA
- binding glycerol: R242 (= R249), E246 (≠ N253), E246 (≠ N253), R250 (≠ T257)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), G10 (= G10), I11 (≠ N11), M12 (= M12), N31 (≠ D31), R32 (≠ V32), N33 (≠ V33), M64 (= M64), L65 (≠ I65), A66 (≠ P66), A70 (≠ H70), T96 (= T96), V121 (= V121), G123 (= G123), T124 (≠ A124), K171 (= K171), S231 (= S229), F232 (= F239), P233 (≠ A240), H236 (≠ M243), K239 (= K246)
2cvzC Structure of hydroxyisobutyrate dehydrogenase from thermus thermophilus hb8 (see paper)
30% identity, 92% coverage: 1:274/299 of query aligns to 1:262/289 of 2cvzC
- active site: S117 (= S122), K165 (= K171), N168 (= N174), N169 (= N175)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), L9 (= L9), G10 (= G10), A11 (≠ N11), M12 (= M12), N30 (≠ Y30), R31 (≠ D31), T32 (≠ V32), C62 (≠ M64), L63 (≠ I65), P64 (= P66), E68 (≠ H70), E71 (= E76), S91 (≠ T96), V116 (= V121), F227 (= F239), K234 (= K246)
1wp4A Structure of tt368 protein from thermus thermophilus hb8 (see paper)
30% identity, 91% coverage: 3:274/299 of query aligns to 2:261/288 of 1wp4A
- active site: S116 (= S122), K164 (= K171), N167 (= N174), N168 (= N175)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G8), L8 (= L9), G9 (= G10), A10 (≠ N11), M11 (= M12), N29 (≠ Y30), R30 (≠ D31), T31 (≠ V32), K34 (≠ A35), C61 (≠ M64), L62 (≠ I65), P63 (= P66), E67 (≠ H70), S90 (≠ T96), V115 (= V121), T225 (≠ G238), F226 (= F239), K233 (= K246)
- binding sulfate ion: S116 (= S122), G117 (= G123), G118 (≠ A124), K164 (= K171)
3pefA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter metallireducens in complex with NADP+ (see paper)
31% identity, 96% coverage: 3:289/299 of query aligns to 3:282/287 of 3pefA
- binding glycerol: D67 (≠ T67), G123 (= G123), K171 (= K171), N175 (= N175), M178 (≠ A178), L203 (≠ Y203), G207 (≠ T207), N213 (≠ G211), A217 (= A215), F232 (= F239), H236 (≠ M243), K239 (= K246), R242 (= R249), R269 (≠ V276)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G10), I11 (≠ N11), M12 (= M12), N31 (≠ D31), R32 (≠ V32), S33 (≠ V33), K36 (≠ A36), M64 (= M64), L65 (≠ I65), A66 (≠ P66), A70 (≠ H70), E73 (≠ A73), T96 (= T96), V121 (= V121), G123 (= G123), S124 (≠ A124), A231 (≠ G238), F232 (= F239), H236 (≠ M243), K239 (= K246)
Query Sequence
>AZOBR_RS26815 FitnessBrowser__azobra:AZOBR_RS26815
MARIAFIGLGNMGRPMCDNLTKAGHDVVGYDVVPAARDAYAAAGGRVASTLAEALADADT
VISMIPTGKHVRAAYEDEGGVLAHARPGTMLIDCSTIDVESARAVSKAAADAGFVMVDAP
VSGAVPAAQAGTLTFMVGGTAEGFERARPVLTAMGPKIFHVGASGNGVALKICNNMMAGM
SMVAISEVFALAEKLGLDHQTVYDVMTVSSGNCWALQSYCPVPGPVPASPANRDYQPGFA
AAMMLKDMRLSQNAAATSGAATPLAGSAAALYQMLVEHGHGNKDFSAVFALITGRLTSA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory