SitesBLAST
Comparing AZOBR_RS27005 FitnessBrowser__azobra:AZOBR_RS27005 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2ztlA Closed conformation of d-3-hydroxybutyrate dehydrogenase complexed with NAD+ and l-3-hydroxybutyrate (see paper)
50% identity, 99% coverage: 1:257/259 of query aligns to 2:258/260 of 2ztlA
- active site: G15 (= G14), N114 (= N113), S142 (= S141), Y155 (= Y154), K159 (= K158), L200 (≠ Q199)
- binding (3s)-3-hydroxybutanoic acid: Q94 (= Q93), S142 (= S141), H144 (≠ L143), K152 (= K151), Y155 (= Y154), G186 (= G185), W187 (≠ T186), L192 (≠ I191), Q196 (= Q195), W257 (≠ Y256)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), T13 (= T12), S14 (≠ T13), G15 (= G14), I16 (= I15), N34 (= N33), G35 (= G34), F36 (= F35), A62 (= A61), D63 (= D62), L64 (= L63), N90 (= N89), A91 (= A90), G92 (= G91), I93 (≠ V92), L113 (≠ V112), I140 (≠ T139), A141 (= A140), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), G186 (= G185), W187 (≠ T186), V188 (= V187), T190 (= T189), P191 (= P190), L192 (≠ I191), V193 (≠ I192)
1wmbA Crystal structure of NAD dependent d-3-hydroxybutylate dehydrogenase (see paper)
50% identity, 99% coverage: 1:257/259 of query aligns to 2:258/260 of 1wmbA
- active site: G15 (= G14), N114 (= N113), S142 (= S141), Y155 (= Y154), K159 (= K158), L200 (≠ Q199)
- binding cacodylate ion: Q94 (= Q93), S142 (= S141), H144 (≠ L143), Y155 (= Y154), G186 (= G185), W257 (≠ Y256)
5b4tA Crystal structure of d-3-hydroxybutyrate dehydrogenase from alcaligenes faecalis complexed with NAD+ and a substrate d-3- hydroxybutyrate (see paper)
49% identity, 99% coverage: 1:257/259 of query aligns to 2:258/260 of 5b4tA
- active site: G15 (= G14), N114 (= N113), S142 (= S141), Y155 (= Y154), K159 (= K158), I200 (≠ Q199)
- binding (3R)-3-hydroxybutanoic acid: Q94 (= Q93), S142 (= S141), H144 (≠ L143), K152 (= K151), Y155 (= Y154), W187 (≠ T186), L192 (≠ I191), Q196 (= Q195), W257 (≠ Y256)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), T13 (= T12), S14 (≠ T13), G15 (= G14), I16 (= I15), N34 (= N33), G35 (= G34), F36 (= F35), A62 (= A61), D63 (= D62), L64 (= L63), N90 (= N89), A91 (= A90), G92 (= G91), I93 (≠ V92), L113 (≠ V112), I140 (≠ T139), A141 (= A140), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), G186 (= G185), W187 (≠ T186), V188 (= V187), T190 (= T189), P191 (= P190), L192 (≠ I191), V193 (≠ I192)
3w8dA Crystal structure of d-3-hydroxybutyrate dehydrogenase from alcaligenes faecalis complexed with NAD+ and an inhibitor methylmalonate
49% identity, 99% coverage: 1:257/259 of query aligns to 2:258/260 of 3w8dA
- active site: G15 (= G14), N114 (= N113), S142 (= S141), Y155 (= Y154), K159 (= K158), I200 (≠ Q199)
- binding methylmalonic acid: Q94 (= Q93), S142 (= S141), H144 (≠ L143), K152 (= K151), Y155 (= Y154), W187 (≠ T186), L192 (≠ I191), Q196 (= Q195), W257 (≠ Y256)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), T13 (= T12), S14 (≠ T13), G15 (= G14), I16 (= I15), N34 (= N33), G35 (= G34), F36 (= F35), A62 (= A61), D63 (= D62), L64 (= L63), N90 (= N89), A91 (= A90), G92 (= G91), I93 (≠ V92), L113 (≠ V112), I140 (≠ T139), A141 (= A140), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), G186 (= G185), W187 (≠ T186), V188 (= V187), T190 (= T189), P191 (= P190), L192 (≠ I191), V193 (≠ I192)
3vdrA Crystal structure of d-3-hydroxybutyrate dehydrogenase, prepared in the presence of the substrate d-3-hydroxybutyrate and NAD(+) (see paper)
49% identity, 99% coverage: 1:257/259 of query aligns to 2:258/260 of 3vdrA
- active site: G15 (= G14), N114 (= N113), S142 (= S141), Y155 (= Y154), K159 (= K158), I200 (≠ Q199)
- binding (3R)-3-hydroxybutanoic acid: Q94 (= Q93), S142 (= S141), H144 (≠ L143), K152 (= K151), Y155 (= Y154), W187 (≠ T186), L192 (≠ I191), Q196 (= Q195), W257 (≠ Y256)
- binding acetoacetic acid: Q94 (= Q93), S142 (= S141), H144 (≠ L143), K152 (= K151), Y155 (= Y154), G186 (= G185), W187 (≠ T186), L192 (≠ I191), Q196 (= Q195), W257 (≠ Y256)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), T13 (= T12), S14 (≠ T13), G15 (= G14), I16 (= I15), F36 (= F35), D63 (= D62), L64 (= L63), N90 (= N89), A91 (= A90), G92 (= G91), L113 (≠ V112), I140 (≠ T139), A141 (= A140), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), G186 (= G185), W187 (≠ T186), V188 (= V187), T190 (= T189), L192 (≠ I191), V193 (≠ I192)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G11 (= G10), T13 (= T12), S14 (≠ T13), G15 (= G14), I16 (= I15), F36 (= F35), D63 (= D62), L64 (= L63), N90 (= N89), A91 (= A90), G92 (= G91), L113 (≠ V112), I140 (≠ T139), A141 (= A140), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), G186 (= G185), W187 (≠ T186), V188 (= V187), T190 (= T189), L192 (≠ I191), V193 (≠ I192)
3vdqA Crystal structure of alcaligenes faecalis d-3-hydroxybutyrate dehydrogenase in complex with NAD(+) and acetate (see paper)
49% identity, 99% coverage: 1:257/259 of query aligns to 2:258/260 of 3vdqA
- active site: G15 (= G14), N114 (= N113), S142 (= S141), Y155 (= Y154), K159 (= K158), I200 (≠ Q199)
- binding acetate ion: Q94 (= Q93), H144 (≠ L143), K152 (= K151), Y155 (= Y154), W187 (≠ T186), L192 (≠ I191), Q196 (= Q195)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), T13 (= T12), S14 (≠ T13), G15 (= G14), I16 (= I15), N34 (= N33), F36 (= F35), A62 (= A61), D63 (= D62), L64 (= L63), S65 (= S64), N90 (= N89), A91 (= A90), G92 (= G91), L113 (≠ V112), I140 (≠ T139), A141 (= A140), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), G186 (= G185), W187 (≠ T186), V188 (= V187), T190 (= T189), P191 (= P190), L192 (≠ I191), V193 (≠ I192)
1x1tA Crystal structure of d-3-hydroxybutyrate dehydrogenase from pseudomonas fragi complexed with NAD+ (see paper)
48% identity, 99% coverage: 1:257/259 of query aligns to 2:234/236 of 1x1tA
- active site: G15 (= G14), N114 (= N113), S142 (= S141), Y155 (= Y154), K159 (= K158)
- binding cacodylate ion: S142 (= S141), H144 (≠ L143), K152 (= K151), Y155 (= Y154), W187 (≠ T186), W233 (≠ Y256)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), T13 (= T12), S14 (≠ T13), G15 (= G14), I16 (= I15), N34 (= N33), G35 (= G34), F36 (= F35), A62 (= A61), D63 (= D62), L64 (= L63), S65 (= S64), N90 (= N89), A91 (= A90), G92 (= G91), L113 (≠ V112), I140 (≠ T139), A141 (= A140), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), G186 (= G185), W187 (≠ T186), V188 (= V187), T190 (= T189)
5yssB Crystal structure of aminocaproic acid cyclase in complex with NAD (+) (see paper)
47% identity, 99% coverage: 1:256/259 of query aligns to 2:252/255 of 5yssB
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), T13 (= T12), S14 (≠ T13), G15 (= G14), I16 (= I15), N34 (= N33), G35 (= G34), F36 (= F35), A58 (= A61), D59 (= D62), L60 (= L63), N86 (= N89), A87 (= A90), G88 (= G91), I89 (≠ V92), I109 (≠ V112), I136 (≠ T139), A137 (= A140), S138 (= S141), Y151 (= Y154), K155 (= K158), P181 (= P184), G182 (= G185), W183 (≠ T186), V184 (= V187), T186 (= T189), P187 (= P190), L188 (≠ I191)
2q2qD Structure of d-3-hydroxybutyrate dehydrogenase from pseudomonas putida (see paper)
48% identity, 99% coverage: 1:256/259 of query aligns to 2:252/255 of 2q2qD
- active site: G15 (= G14), S138 (= S141), Y151 (= Y154), K155 (= K158), R196 (≠ Q199)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), T13 (= T12), S14 (≠ T13), G15 (= G14), I16 (= I15), N34 (= N33), G35 (= G34), F36 (= F35), A58 (= A61), D59 (= D62), L60 (= L63), N86 (= N89), A87 (= A90), G88 (= G91), I89 (≠ V92), L109 (≠ V112), I136 (≠ T139), A137 (= A140), S138 (= S141), Y151 (= Y154), K155 (= K158), P181 (= P184), G182 (= G185), W183 (≠ T186), V184 (= V187), T186 (= T189), P187 (= P190), L188 (≠ I191), V189 (≠ I192)
6zzsD Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and 3-oxovalerate (see paper)
38% identity, 100% coverage: 1:259/259 of query aligns to 5:261/261 of 6zzsD
- active site: G18 (= G14), S143 (= S141), Y156 (= Y154)
- binding nicotinamide-adenine-dinucleotide: G14 (= G10), S17 (≠ T13), G18 (= G14), I19 (= I15), D38 (≠ G34), M39 (≠ F35), C63 (≠ A61), D64 (= D62), V65 (≠ L63), N91 (= N89), A92 (= A90), G93 (= G91), F94 (≠ V92), V114 (= V112), M141 (≠ T139), A142 (= A140), S143 (= S141), Y156 (= Y154), K160 (= K158), P186 (= P184), G187 (= G185), Y188 (≠ T186), V189 (= V187), T191 (= T189), L193 (≠ I191), V194 (≠ I192)
- binding 3-oxidanylidenepentanoic acid: Q95 (= Q93), S143 (= S141), N145 (≠ L143), K153 (= K151), Y156 (= Y154), L193 (≠ I191), Q197 (= Q195)
6zzqA Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and acetoacetate (see paper)
38% identity, 100% coverage: 1:259/259 of query aligns to 4:260/260 of 6zzqA
- active site: G17 (= G14), S142 (= S141), Y155 (= Y154)
- binding acetoacetic acid: F93 (≠ V92), Q94 (= Q93), K109 (≠ L108), V113 (= V112), S142 (= S141), N144 (≠ L143), K152 (= K151), Y155 (= Y154), Y187 (≠ T186), Q196 (= Q195)
- binding nicotinamide-adenine-dinucleotide: G13 (= G10), S16 (≠ T13), G17 (= G14), I18 (= I15), D37 (≠ G34), M38 (≠ F35), C62 (≠ A61), D63 (= D62), V64 (≠ L63), N90 (= N89), A91 (= A90), G92 (= G91), V113 (= V112), M140 (≠ T139), A141 (= A140), S142 (= S141), Y155 (= Y154), K159 (= K158), P185 (= P184), G186 (= G185), Y187 (≠ T186), V188 (= V187), T190 (= T189), L192 (≠ I191), V193 (≠ I192)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
37% identity, 97% coverage: 4:255/259 of query aligns to 5:243/246 of 3osuA
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
36% identity, 97% coverage: 4:255/259 of query aligns to 2:236/239 of 3sj7A
- active site: G12 (= G14), S138 (= S141), Q148 (≠ K151), Y151 (= Y154), K155 (= K158)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G10), S10 (≠ T12), R11 (≠ T13), G12 (= G14), I13 (= I15), N31 (= N33), Y32 (≠ G34), A33 (≠ F35), G34 (= G36), S35 (≠ D37), A58 (= A61), N59 (≠ D62), V60 (≠ L63), N86 (= N89), A87 (= A90), G88 (= G91), I89 (≠ V92), T109 (≠ V112), L136 (≠ T139), S137 (≠ A140), S138 (= S141), Y151 (= Y154), K155 (= K158), P181 (= P184), G182 (= G185), F183 (≠ T186), I184 (≠ V187)
3sjuA Hedamycin polyketide ketoreductase bound to NADPH (see paper)
40% identity, 99% coverage: 4:259/259 of query aligns to 1:255/255 of 3sjuA
- active site: G11 (= G14), S138 (= S141), Y151 (= Y154), K155 (= K158), Y196 (≠ Q199)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G10), S9 (≠ T12), S10 (≠ T13), G11 (= G14), I12 (= I15), A31 (≠ G36), R32 (≠ D37), D33 (≠ R38), C56 (≠ A61), D57 (= D62), V58 (≠ L63), T59 (≠ S64), S84 (≠ N89), A85 (= A90), G86 (= G91), T107 (≠ V112), I136 (≠ T139), A137 (= A140), S138 (= S141), Y151 (= Y154), K155 (= K158), P181 (= P184), G182 (= G185), Y183 (≠ T186), V184 (= V187), T186 (= T189), M188 (≠ I191)
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
36% identity, 99% coverage: 1:257/259 of query aligns to 3:246/248 of 6ixmC