SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AZOBR_RS27540 FitnessBrowser__azobra:AZOBR_RS27540 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 15 hits to proteins with known functional sites (download)

4jxrB Crystal structure of a gnat superfamily phosphinothricin acetyltransferase (pat) from sinorhizobium meliloti in complex with accoa
39% identity, 92% coverage: 6:179/189 of query aligns to 1:175/185 of 4jxrB

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4jxrB

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binding acetyl coenzyme *a: A29 (≠ G34), D86 (≠ H90), S87 (= S91), I88 (= I92), Y89 (= Y93), L90 (≠ V94), P92 (≠ K96), R95 (≠ Q99), G96 (= G100), G100 (= G104), H127 (≠ N131), S129 (≠ P133), S130 (= S134), H134 (= H138)

5dwnA Crystal structure of phosphinothricin n-acetyltransferase from brucella ovis in complex with acetylcoa
41% identity, 89% coverage: 10:177/189 of query aligns to 6:175/181 of 5dwnA

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binding acetyl coenzyme *a: S87 (= S91), I88 (= I92), Y89 (= Y93), I90 (≠ V94), K95 (≠ Q99), G96 (= G100), G98 (= G102), G100 (= G104), K101 (≠ R105), G131 (≠ P133), S132 (= S134), K134 (≠ R136), L135 (= L137), H136 (= H138)

3dr8A Structure of ynca, a putative acetyltransferase from salmonella typhimurium with its cofactor acetyl-coa
39% identity, 91% coverage: 7:178/189 of query aligns to 2:171/173 of 3dr8A

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binding acetyl coenzyme *a: A29 (≠ G34), H85 (= H90), S86 (= S91), V87 (≠ I92), Y88 (= Y93), V89 (= V94), Q94 (= Q99), G95 (= G100), G97 (= G102), G99 (= G104), R100 (= R105), A127 (≠ T132), A128 (≠ P133), R131 (= R136), H133 (= H138), S135 (≠ A140)

Q8ZPD3 L-methionine sulfoximine/L-methionine sulfone acetyltransferase; L-amino acid N-acyltransferase; Methionine derivative detoxifier A; MDDA; EC 2.3.1.-; EC 2.3.1.- from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
39% identity, 90% coverage: 8:178/189 of query aligns to 1:169/171 of Q8ZPD3

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E82 (= E89) mutation to Q: Loss of acetyltransferase activity.
VYV 85:87 (≠ IYV 92:94) binding
GKGLGR 93:98 (≠ GLGIGR 100:105) binding
N124 (= N131) binding
S133 (≠ A140) binding

5t7eD Crystal structure of streptomyces hygroscopicus bialaphos resistance (bar) protein in complex with coenzyme a and l-phosphinothricin (see paper)
39% identity, 83% coverage: 10:166/189 of query aligns to 4:159/175 of 5t7eD

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binding coenzyme a: Y22 (≠ H28), V28 (≠ G34), V85 (≠ I92), Y86 (= Y93), V87 (= V94), Q92 (= Q99), R93 (≠ G100), T94 (≠ L101), G95 (= G102), G97 (= G104), S98 (≠ R105), P126 (= P133), S127 (= S134), M130 (≠ L137)
binding phosphinothricin: F30 (= F36), K72 (≠ R79), R74 (= R81), Y77 (= Y84), T84 (≠ S91), Y86 (= Y93), V119 (= V126), G121 (≠ D128)

5t7dA Crystal structure of streptomyces hygroscopicus bialaphos resistance (bar) protein in complex with acetyl coenzyme a (see paper)
39% identity, 83% coverage: 10:166/189 of query aligns to 3:158/173 of 5t7dA

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binding acetyl coenzyme *a: Y21 (≠ H28), V27 (≠ G34), T83 (≠ S91), V84 (≠ I92), Y85 (= Y93), V86 (= V94), Q91 (= Q99), R92 (≠ G100), G94 (= G102), G96 (= G104), S97 (≠ R105), V118 (= V126), P125 (= P133), S126 (= S134), R128 (= R136), M129 (≠ L137)

2jlmF Structure of a putative acetyltransferase (aciad1637) from acinetobacter baylyi adp1 (see paper)
36% identity, 79% coverage: 21:169/189 of query aligns to 22:171/180 of 2jlmF

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I

E

K

A

R

C

A

T

V

A

E

A

S

D

E

C

V

R

H

Q

V

M

M

V

V

A

G

V

C

V

I

D
|
D

S

A

A

T

N
|
N

T

V

P

A

S

S

L

I

R

Q

L

L

H

H

E

Q

A

K

F

L

G

G

F

F

V

I

R

H

A

S

G

G

V

T

L

I

R

Q

S

Q

V

A

G

G

F

F

R

K

F

F

G

G

R

R

W

W

T

L

D

D

S

A

V

A

F

F

L

Y

Q

Q

R

L

S

T

L

L

binding azide ion: T34 (≠ L33), A35 (≠ G34), Y95 (= Y93), I96 (≠ V94), D130 (= D128), N133 (= N131)

4jwpA Crystal structure of ribosomal-protein-alanine n-acetyltransferase from brucella melitensis in complex with acetyl coa
38% identity, 87% coverage: 5:169/189 of query aligns to 1:165/165 of 4jwpA

query
sites
4jwpA

S

S

F

M

S

T

I

L

T

L

I

I

R

R

P

H

S

A

T

T

E

E

A

A

D

D

V

L

A

P

A

A

M

L

L

L

E

A

I

I

Y

Y

S

N

Y

D

H

A

I

V

Q

E

H

N

G

T

L

L

G
x
A

P

I

F

W

-

N

-

E

-

T

-

L

-

V

D

D

I

L

E

E

P

N

L

R

H

H

P

Q

E

W

E

L

L

E

K

N

R

R

R

N

R

R

K

D

A

G

M

-

L

-

K

-

R

-

L

F

P

P

H

V

L

L

V

V

A

A

E

E

I

R

G

E

G

G

V

Q

V

V

G

G

Y

Y

A

A

Y

S

A

Y

A

G

P

P

F

F

R

R

K

P

R

F

P

E

A

G

Y

F

R

R

Y

H

T

S

V

S

E

E

H

L

S

S

I
x
V

Y
|
Y

V
|
V

H

A

K

S

D

N

R

A

Q
x
R

G
|
G

L

G

G
|
G

I

I

G
|
G

R
|
R

R

T

L

L

P

A

A

E

L

L

I

I

E

E

A
x
E

C

A

T

R

A
x
E

A

R

D

K

C

V

R

H

Q

V

M

L

V

I

A

A

V
x
G

V

I

D

E

S

A

A

G

N

N

T

A

P
x
A

S
|
S

L

I

R
x
A

L

L

H

H

E

R

A

S

F

Q

G

G

F

F

V

E

R

E

A

C

G

G

V

T

L

L

R

K

S

Q

V

V

G

G

F

Q

R

K

F

F

G

G

R

R

W

W

T

L

D

D

S

L

V

L

F

F

L

M

Q

Q

R

K

S

I

L

L

binding acetyl coenzyme *a: A30 (≠ G34), V88 (≠ I92), Y89 (= Y93), V90 (= V94), R95 (≠ Q99), G96 (= G100), G98 (= G102), G100 (= G104), R101 (= R105), G122 (≠ V126), A129 (≠ P133), S130 (= S134), A132 (≠ R136)
binding calcium ion: E110 (≠ A114), E113 (≠ A117)

4mbuA Crystal structure of n-acetyltransferase from staphylococcus aureus mu50 (see paper)
36% identity, 85% coverage: 10:169/189 of query aligns to 5:164/165 of 4mbuA

query
sites
4mbuA

I

I

R

R

P

C

S

A

T

K

E

K

A

E

D

D

V

L

A

N

A

A

M

I

L

L

E

A

I

I

Y

Y

S

N

Y

D

H

A

I

I

Q

I

H

N

G

T

L

T

G

A

P

V

F

Y

D

T

I

Y

E

K

P

P

L

Q

H

T

P

I

E

D

E

E

L

R

K

I

R

A

R

W

R

F

K

E

A

T

M

K

L

Q

K

R

R

N

R

H

L

E

P

P

H

I

L

F

V

V

A

F

E

E

I

E

G

N

G

G

V

S

V

V

V

L

G

G

Y

F

A

A

Y

T

A

F

A

G

P

S

F

F

R

R

K

P

R

W

P

P

A

A

Y

Y

R

Q

Y

Y

T

T

V

I

E

E

H

H

S

S

I

I

Y

Y

V

V

H

D

K

A

D

S

R

A

Q

R

G

G

L

K

G

G

I

I

G

A

R

S

R

Q

L

L

P

Q

A

R

L

L

I

I

E

V

A

E

C

A

T

K

A

A

A

K

D

G

C

Y

R

R

Q

T

M

L

V

V

A

A

V

G

V

I

D

D

S

A

A

S

N

N

T

E

P

A

S

S

L

I

R

K

L

L

H

H

E
x
Q

A

K

F

F

G

N

F

F

V
x
K

R
x
H

A

A

G

G

V

T

L

L

R

T

S

N

V

V

G

G

F

Y

R

K

F

F

G

D

R

Y

W

W

T

L

D

D

S

L

V

A

F

F

L

Y

Q

E

R

L

S

D

L

L

binding phosphate ion: Q134 (≠ E139), K139 (≠ V144), H140 (≠ R145)

A6VCX3 L-methionine sulfoximine/L-methionine sulfone acetyltransferase; Methionine derivative detoxifier A; MDDA; EC 2.3.1.- from Pseudomonas aeruginosa (strain PA7) (see paper)
36% identity, 87% coverage: 6:169/189 of query aligns to 1:165/172 of A6VCX3

query
sites
A6VCX3

F

M

S

S

I

A

T

S

I

I

R

R

P

D

S

A

T

G

E

V

A

A

D

D

V

L

A

P

A

G

M

I

L

L

E

A

I

I

Y

Y

S

N

Y

D

H

A

I

V

Q

G

H

N

G

T

L

T

G

A

P

I

F

W

D

N

I

E

E

T

P

P

L

V

H

D

P

L

E

A

E

N

L

R

K

Q

R

A

R

W

R

F

K

D

A

A

M

R

L

A

K

R

R

Q

R

G

L

Y

P

P

H

I

L

L

V

V

A

A

-

S

E

D

I

A

G

A

G

G

V

E

V

V

V

L

G

G

Y

Y

A

A

Y

S

A

Y

A

G

P

D

F

W

R
|
R

K
x
P

R
x
F

P

E

A

G

Y

F

R

R

Y

G

T

T

V

V

E
|
E

H
|
H

S
|
S

I

V

Y

Y

V

V

H

R

K

D

D

D

R

Q

Q

R

G

G

L

K

G

G

I

L

G

G

R

V

R

Q

L

L

P

Q

A

A

L

L

I

I

E

E

A

R

C

A

T

R

A

A

A

Q

D

G

C

L

R

H

Q

V

M

M

V

V

A

A

V

A

V

I

D

E

S

S

A

G

N

N

T

A

P

A

S

S

L

I

R

G

L

L

H

H

E

R

A

R

F

L

G

G

F

F

V

E

R

I

A

S

G

G

V

Q

L

M

R

P

S

Q

V

V

G

G

F

Q

R

K

F

F

G

G

R

R

W

W

T

L

D

D

S

L

V

T

F

F

L

M

Q

Q

R

L

S

N

L

L

RPF 75:77 (≠ RKR 79:81) binding
EHS 85:87 (= EHS 89:91) binding

2j8mA Structure of p. Aeruginosa acetyltransferase pa4866 (see paper)
37% identity, 86% coverage: 7:169/189 of query aligns to 1:164/171 of 2j8mA

query
sites
2j8mA

S

S

I

A

T

S

I

I

R

R

P

D

S

A

T

G

E

V

A

A

D

D

V

L

A

P

A

G

M

I

L

L

E

A

I

I

Y

Y

S

N

Y

D

H

A

I

V

Q

G

H

N

G

T

L

T

G

A

P

I

F

W

D

N

I

E

E

T

P

P

L

V

H

D

P

L

E

A

E

N

L

R

K

Q

R

A

R

W

R

F

K

D

A

A

M

R

L

A

K

R

R

Q

R

G

L

Y

P

P

H

I

L

L

V

V

A

A

-

S

E

D

I

A

G

A

G

G

V

E

V

V

V

L

G

G

Y

Y

A

A

Y

S

A

Y

A

G

P

D

F
x
W

R
|
R

K

P

R

F

P

E

A

G

Y

F

R

R

Y

G

T

T

V

V

E

E

H

H

S

S

I

V

Y

Y

V

V

H

R

K

D

D

D

R

Q

Q

R

G

G

L

K

G

G

I

L

G

G

R

V

R

Q

L

L

P

Q

A

A

L

L

I

I

E

E

A

R

C

A

T

R

A

A

A

Q

D

G

C

L

R

H

Q

V

M

M

V

V

A

A

V

A

V

I

D

E

S

S

A

G

N

N

T

A

P

A

S

S

L

I

R

G

L

L

H

H

E

R

A

R

F

L

G

G

F

F

V

E

R

I

A

S

G

G

V

Q

L

M

R

P

S

Q

V

V

G

G

F

Q

R

K

F

F

G

G

R

R

W

W

T

L

D

D

S

L

V

T

F

F

L

M

Q

Q

R

L

S

N

L

L

binding azide ion: W73 (≠ F78), R74 (= R79)

2j8rA Structure of p. Aeruginosa acetyltransferase pa4866 solved in complex with l-methionine sulfoximine (see paper)
36% identity, 85% coverage: 9:169/189 of query aligns to 2:163/170 of 2j8rA

query
sites
2j8rA

T

S

I

I

R

R

P

D

S

A

T

G

E

V

A

A

D

D

V

L

A

P

A

G

M

I

L

L

E

A

I

I

Y

Y

S

N

Y

D

H

A

I

V

Q

G

H

N

G

T

L

T

G
x
A

P
x
I

F
x
W

D

N

I

E

E

T

P

P

L

V

H

D

P

L

E

A

E

N

L

R

K

Q

R

A

R
x
W

R

F

K

D

A

A

M

R

L

A

K

R

R

Q

R

G

L

Y

P

P

H

I

L

L

V

V

A

A

-

S

E

D

I

A

G

A

G

G

V

E

V

V

V

L

G

G

Y
|
Y

A

A

Y

S

A

Y

A

G

P

D

F

W

R
|
R

K

P

R
x
F

P

E

A

G

Y
x
F

R

R

Y

G

T

T

V

V

E
|
E

H

H

S
|
S

I

V

Y

Y

V

V

H

R

K

D

D

D

R

Q

Q

R

G

G

L

K

G

G

I

L

G

G

R

V

R

Q

L

L

P

Q

A

A

L

L

I

I

E

E

A

R

C

A

T

R

A

A

A

Q

D

G

C

L

R

H

Q

V

M

M

V

V

A

A

V

A

V

I

D

E

S

S

A

G

N

N

T

A

P

A

S

S

L

I

R

G

L

L

H

H

E

R

A

R

F

L

G

G

F

F

V

E

R

I

A

S

G

G

V

Q

L

M

R

P

S

Q

V

V

G

G

F

Q

R

K

F

F

G

G

R

R

W

W

T

L

D

D

S

L

V

T

F

F

L

M

Q

Q

R

L

S

N

L

L

binding azide ion: W42 (≠ R49), Y66 (= Y72), S85 (= S91)
binding (2s)-2-amino-4-(methylsulfonimidoyl)butanoic acid: A27 (≠ G34), I28 (≠ P35), W29 (≠ F36), R73 (= R79), F75 (≠ R81), F78 (≠ Y84), E83 (= E89)

5wphA Crystal structure of arsn, n-acetyltransferase with substrate ast from pseudomonas putida kt2440 (see paper)
32% identity, 87% coverage: 8:171/189 of query aligns to 2:164/179 of 5wphA

query
sites
5wphA

I

I

T

D

I

I

R

R

P

V

S

A

T

R

E

P

A

E

D

D

V

A

A

E

M

I

L

Q

E

I

I

I

Y

Y

S

A

Y

P

H

I

I

V

Q

L

H

N

G

T

L

A

G

I

P
x
S

F
|
F

D

E

I

-

E

E

P

A

L

V

H

P

P

S

E

V

E

E

L

Q

K

M

R

R

R

E

R

R

K

I

A

S

M

T

L

T

K

L

R

Q

R

T

L

Y

P

P

H

Y

L

L

V

V

A

A

E

V

I

R

G

E

G

G

V

R

V

V

G

G

Y

Y

A

A

Y

Y

A

A

A

S

P

Q

F

H

R
|
R

K

A

R
|
R

P

A

A

A

Y
|
Y

R

R

Y

W

T

A

V

V

E

D

H

V

S

T

I

V

Y

Y

V

V

H

A

K

E

D

G

R

Q

Q

R

G

R

L

S

G

G

I

I

G

A

R

R

R

Q

L

L

L

Y

P

D

A

V

L

L

I

L

E

P

A

V

C

L

T

K

A

R

A

L

D

G

C

Y

R

R

Q

S

M

A

V

Y

A

A

V

G

V

I

D

A

S

L

A

P

N

N

T

E

P

G

S

S

L

V

R

G

L

L

H

H

E

E

A

R

F

L

G

G

F

F

V

Q

R

H

A

I

G

G

V

T

L

F

R

P

S

Q

V

V

G

G

F

F

R

K

F

L

G

D

R

A

W

W

T

H

D

D

S

V

V

G

F

Y

L

W

Q

R

R

F

S

D

L

F

G

G

E

D

binding (2S)-2-amino-4-[hydroxy(methyl)arsoryl]butanoic acid: S29 (≠ P35), F30 (= F36), R72 (= R79), R74 (= R81), Y77 (= Y84)

6m7gA Crystal structure of arsn, n-acetyltransferase with substrate phosphinothricin from pseudomonas putida kt2440 (see paper)
32% identity, 87% coverage: 8:171/189 of query aligns to 2:164/176 of 6m7gA

query
sites
6m7gA

I

I

T

D

I

I

R

R

P

V

S

A

T

R

E

P

A

E

D

D

V

A

A

E

M

I

L

Q

E

I

I

I

Y

Y

S

A

Y

P

H

I

I

V

Q

L

H

N

G

T

L

A

G

I

P

S

F

F

D

E

I

-

E

E

P

A

L

V

H

P

P

S

E

V

E

E

L

Q

K

M

R

R

R

E

R

R

K

I

A

S

M

T

L

T

K

L

R

Q

R

T

L

Y

P

P

H

Y

L

L

V

V

A

A

E

V

I

R

G

E

G

G

V

R

V

V

G

G

Y

Y

A

A

Y

Y

A

A

A

S

P

Q

F

H

R

R

K

A

R
|
R

P

A

A

A

Y
|
Y

R

R

Y

W

T

A

V

V

E

D

H

V

S

T

I

V

Y

Y

V

V

H

A

K

E

D

G

R

Q

Q

R

G

R

L

S

G

G

I

I

G

A

R

R

R

Q

L

L

L

Y

P

D

A

V

L

L

I

L

E

P

A

V

C

L

T

K

A

R

A

L

D

G

C

Y

R

R

Q

S

M

A

V

Y

A

A

V

G

V

I

D

A

S

L

A

P

N

N

T

E

P

G

S

S

L

V

R

G

L

L

H

H

E

E

A

R

F

L

G

G

F

F

V

Q

R

H

A

I

G

G

V

T

L

F

R

P

S

Q

V

V

G

G

F

F

R

K

F

L

G

D

R

A

W

W

T

H

D

D

S

V

V

G

F

Y

L

W

Q

R

R

F

S

D

L

F

G

G

E

D

binding phosphinothricin: R74 (= R81), Y77 (= Y84)

2vi7C Structure of a putative acetyltransferase (pa1377)from pseudomonas aeruginosa (see paper)
34% identity, 59% coverage: 57:167/189 of query aligns to 52:162/165 of 2vi7C

query
sites
2vi7C

R

R

L

L

P

L

H

I

L

L

V

V

A

A

E

L

I

H

G

Q

G

G

V

D

V

V

V

I

G

G

Y

-

A

-

Y

-

A

S

A

A

P

S

F

L

R

E

K

Q

R

H

P

P

A
x
R

Y
x
I

R

R

Y

R

T

S

V

H

E

S

H

G

S

S

I

I

Y

G

-

M

-

G

V

V

H

A

K

V

D

A

R

W

Q

Q

G

G

L

K

G

G

I

V

G

G

R

S

R

R

L

L

P

G

A

E

L

L

I

L

E

D

-

I

A

A

C

D

T

N

A

W

A

M

D

N

C

L

R

R

Q

R

M

V

V

E

A

L

V

T

V

V

D

Y

S

T

A

D

N

N

T

A

P

P

S

A

L

L

R

A

L

L

H

Y

E

R

A

K

F

F

G

G

F

F

V

E

R

T

A

E

G

G

V

E

L

M

R

R

S

D

V

Y

G

A

F

V

R

R

F

D

G

G

R

R

W

F

T

V

D

D

S

V

V

Y

F

S

L

M

Q

A

R

R

binding azide ion: R75 (≠ A83), I76 (≠ Y84)

Query Sequence

>AZOBR_RS27540 FitnessBrowser__azobra:AZOBR_RS27540
VFDTSFSITIRPSTEADVAAMLEIYSYHIQHGLGPFDIEPLHPEELKRRRKAMLKRRLPH
LVAEIGGVVVGYAYAAPFRKRPAYRYTVEHSIYVHKDRQGLGIGRRLLPALIEACTAADC
RQMVAVVDSANTPSLRLHEAFGFVRAGVLRSVGFRFGRWTDSVFLQRSLGEADAELPDDV
RGGTVQAGD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory