SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AZOBR_RS29310 FitnessBrowser__azobra:AZOBR_RS29310 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0AC81 Lactoylglutathione lyase; Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase; EC 4.4.1.5 from Escherichia coli (strain K12) (see paper)
43% identity, 96% coverage: 6:141/141 of query aligns to 1:129/135 of P0AC81

query
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P0AC81

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H5 (= H10) binding
E56 (= E66) binding
H74 (= H85) binding in other chain
E122 (= E134) binding in other chain

1fa6A Crystal structure of the co(ii)-bound glyoxalase i of escherichia coli (see paper)
43% identity, 95% coverage: 6:139/141 of query aligns to 1:127/128 of 1fa6A

query
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1fa6A

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active site: H5 (= H10), E56 (= E66), H74 (= H85), E122 (= E134)
binding cobalt (ii) ion: H5 (= H10), E56 (= E66), H74 (= H85), E122 (= E134)

1fa5A Crystal structure of the zn(ii)-bound glyoxalase i of escherichia coli (see paper)
43% identity, 95% coverage: 6:139/141 of query aligns to 1:127/128 of 1fa5A

query
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active site: H5 (= H10), E56 (= E66), H74 (= H85), E122 (= E134)
binding zinc ion: H5 (= H10), E56 (= E66), H74 (= H85), E122 (= E134)

4mttA Ni- and zn-bound gloa2 at low resolution (see paper)
41% identity, 94% coverage: 6:138/141 of query aligns to 1:126/128 of 4mttA

query
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active site: H5 (= H10), E56 (= E66), H74 (= H85), E122 (= E134)
binding zinc ion: H5 (= H10), E56 (= E66)

6bnnA Crystal structure of v278e-glyoxalase i mutant from zea mays in space group p4(1)2(1)2 (see paper)
35% identity, 94% coverage: 7:139/141 of query aligns to 16:141/282 of 6bnnA

query
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6bnnA

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active site: H19 (= H10), E70 (= E66), H88 (= H85), E136 (= E134)
binding cobalt (ii) ion: H88 (= H85), E136 (= E134)
binding glutathione: R112 (= R110), V117 (≠ Q115), K118 (= K116)

Sites not aligning to the query:

5d7zA Crystal structure of glyoxalase i from zea mays (see paper)
35% identity, 94% coverage: 7:139/141 of query aligns to 10:135/281 of 5d7zA

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5d7zA

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active site: H13 (= H10), E64 (= E66), H82 (= H85), E130 (= E134)

Sites not aligning to the query:

binding nickel (ii) ion: 276, 281

Q948T6 Lactoylglutathione lyase; Aldoketomutase; Allergen Glb33; Glyoxalase I; Glx I; Glyoxylase I 11; OsGLYI-11; OsGLYI11; Ketone-aldehyde mutase; Methylglyoxalase; PP33; S-D-lactoylglutathione methylglyoxal lyase; Allergen Ory s Glyoxalase I; EC 4.4.1.5 from Oryza sativa subsp. japonica (Rice) (see paper)
35% identity, 94% coverage: 7:139/141 of query aligns to 24:150/291 of Q948T6

query
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Q948T6

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E78 (= E66) mutation to Q: No effect on the activity.
E145 (= E134) mutation to Q: Loss of activity.

Sites not aligning to the query:

209 E→D: Loss of activity.

P50107 Glyoxalase I; Glx I; Aldoketomutase; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase; actoylglutathione lyase; EC 4.4.1.5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
36% identity, 92% coverage: 7:136/141 of query aligns to 182:320/326 of P50107

query
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P50107

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E318 (= E134) active site, Proton donor/acceptor

Sites not aligning to the query:

163 active site, Proton donor/acceptor

2c21A Specificity of the trypanothione-dependednt leishmania major glyoxalase i: structure and biochemical comparison with the human enzyme (see paper)
34% identity, 93% coverage: 7:137/141 of query aligns to 3:121/139 of 2c21A

query
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2c21A

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V

Y

F

V

L

G

G

Y

Y

G

G

T

P

A

-

E

-

D

-

P

-

L

-

D

E

S

M

P

S

A

S

M

T

V

V

I

L

E
|
E

L

L

V

T

A

Y

N

N

W

Y

T

G

Q

V

E

-

V

T

P

S

Y

Y

S

K

Q

H

G

D

S

E

A

A

F

Y

G

G

H
|
H

I

I

A

A

I

I

G

G

V

V

P

E

S

D

G

-

I

V

G

K

A

E

L

L

C

V

E

A

R

D

L

M

A

R

A

K

E

H

G

D

V

V

P

P

V

I

P

D

R

Y

P

E

P

D

K

E

P

S

Q

-

K

-

H

-

G

-

D

-

N

G

I

F

V

M

A

A

F

F

I

V

E

V

D

D

P

P

D

D

G

G

Y

Y

R

Y

I

I

E
|
E

L

L

V

L

Q

N

active site: H6 (= H10), E57 (= E66), H75 (= H85), E118 (= E134)
binding nickel (ii) ion: H6 (= H10), E57 (= E66), H75 (= H85), E118 (= E134)

3w0uA Human glyoxalase i with an n-hydroxypyridone inhibitor
36% identity, 94% coverage: 5:137/141 of query aligns to 21:168/175 of 3w0uA

query
sites
3w0uA

D

D

I

F

K

L

L

L

L

Q

H
x
Q

A

T

M

M

L

L

R

R

V

V

S

K

D

D

M

P

E

K

R

K

S

S

L

L

D

D

F

F

Y

Y

T

T

R

R

L

V

L

L

G

G

M

M

R

T

V

L

I

I

Q

Q

Q

K

R

C

E

D

H

F

K

P

K

I

N

M

Q

K

F
|
F

T

S

Q
x
L

A

Y

Y

F

V

L

G

A

Y

Y

G

-

T

-

A

-

E

E

D

D

P

K

L

N

D

D

S

I

P

P

-

K

-

E

-

K

-

D

-

E

-

K

-

I

-

A

-

W

-

A

-

L

-

S

-

R

A

K

M

A

V

T

I

L

E
|
E

L

L

V

T

A

H

N

N

W

W

T

G

Q

T

E

E

-

D

-

E

-

T

V

Q

P

S

Y

Y

S

H

Q

N

G

G

S

N

A

S

-

D

-

P

-

R

-

G

F

F

G

G

H
|
H

I

I

A

G

I

I

G

A

V

V

P

P

S

D

G

V

I

Y

G

S

A

A

L

-

C

C

E

K

R

R

L

F

A

E

E

L

G

G

V

V

P

K

V

F

P

V

R
x
K

P

K

P

P

K

D

P

D

Q

G

K

K

H
x
M

G

K

D

G

N

-

I

-

V

L

A

A

F

F

I

I

E

Q

D

D

P

P

D

D

G

G

Y

Y

R

W

I

I

E
|
E

L

I

V

L

Q

N

active site: Q26 (≠ H10), E92 (= E66), H119 (= H85), E165 (= E134)
binding N-[3-(1-Hydroxy-6-oxo-4-phenyl-1,6-dihydro-pyridin-2-yl)-5-methanesulfonylamino-phenyl]-methanesulfonamide: Q26 (≠ H10), F60 (= F44), L62 (≠ Q46), E92 (= E66), H119 (= H85), K143 (≠ R110), M150 (≠ H117), E165 (= E134)
binding zinc ion: Q26 (≠ H10), E92 (= E66), H119 (= H85), E165 (= E134)

Sites not aligning to the query:

binding N-[3-(1-Hydroxy-6-oxo-4-phenyl-1,6-dihydro-pyridin-2-yl)-5-methanesulfonylamino-phenyl]-methanesulfonamide: 172

3w0tA Human glyoxalase i with an n-hydroxypyridone derivative inhibitor
36% identity, 94% coverage: 5:137/141 of query aligns to 21:168/176 of 3w0tA

query
sites
3w0tA

D

D

I

F

K

L

L

L

L

Q

H
x
Q

A

T

M

M

L

L

R

R

V

V

S

K

D

D

M

P

E

K

R

K

S

S

L

L

D

D

F

F

Y

Y

T

T

R

R

L

V

L

L

G

G

M

M

R

T

V

L

I

I

Q

Q

Q

K

R

C

E

D

H
x
F

K

P

K

I

N

M

Q

K

F
|
F

T

S

Q
x
L

A

Y

Y
x
F

V

L

G

A

Y

Y

G

-

T

-

A

-

E

E

D

D

P

K

L

N

D

D

S

I

P

P

-

K

-

E

-

K

-

D

-

E

-

K

-

I

-

A

-

W

-

A

-

L

-

S

-

R

A

K

M

A

V

T

I

L

E
|
E

L

L

V

T

A

H

N

N

W

W

T

G

Q

T

E

E

-

D

-

E

-

T

V

Q

P

S

Y

Y

S

H

Q

N

G

G

S

N

A

S

-

D

-

P

-

R

-

G

F

F

G

G

H
|
H

I

I

A

G

I

I

G

A

V

V

P

P

S

D

G

V

I

Y

G

S

A

A

L

-

C

C

E

K

R

R

L

F

A

E

E

L

G

G

V

V

P

K

V

F

P

V

R

K

P

K

P

P

K

D

P

D

Q

G

K

K

H

M

G

K

D

G

N

-

I

-

V

L

A

A

F

F

I

I

E

Q

D

D

P

P

D

D

G

G

Y

Y

R

W

I

I

E
|
E

L

I

V

L

Q

N

active site: Q26 (≠ H10), E92 (= E66), H119 (= H85), E165 (= E134)
binding N-[3-(1-hydroxy-6-oxo-4-phenyl-1,6-dihydropyridin-2-yl)phenyl]methanesulfonamide: Q26 (≠ H10), F55 (≠ H39), F60 (= F44), L62 (≠ Q46), F64 (≠ Y48), E92 (= E66), H119 (= H85), E165 (= E134)
binding zinc ion: Q26 (≠ H10), E92 (= E66), H119 (= H85), E165 (= E134)

Sites not aligning to the query:

binding N-[3-(1-hydroxy-6-oxo-4-phenyl-1,6-dihydropyridin-2-yl)phenyl]methanesulfonamide: 172, 176

3vw9A Human glyoxalase i with an n-hydroxypyridone inhibitor (see paper)
36% identity, 94% coverage: 5:137/141 of query aligns to 21:168/176 of 3vw9A

query
sites
3vw9A

D

D

I

F

K

L

L

L

L

Q

H
x
Q

A

T

M

M

L

L

R

R

V

V

S

K

D

D

M

P

E

K

R

K

S

S

L

L

D

D

F

F

Y

Y

T

T

R

R

L

V

L

L

G

G

M

M

R

T

V

L

I

I

Q

Q

Q

K

R
x
C

E

D

H

F

K

P

K

I

N

M

Q

K

F

F

T

S

Q
x
L

A

Y

Y

F

V

L

G

A

Y

Y

G

-

T

-

A

-

E

E

D

D

P

K

L

N

D

D

S

I

P

P

-

K

-

E

-

K

-

D

-

E

-

K

-

I

-

A

-

W

-

A

-

L

-

S

-

R

A

K

M

A

V

T

I

L

E
|
E

L

L

V

T

A

H

N

N

W

W

T

G

Q

T

E

E

-

D

-

E

-

T

V

Q

P

S

Y

Y

S

H

Q

N

G

G

S

N

A

S

-

D

-

P

-

R

-

G

F

F

G

G

H
|
H

I

I

A

G

I

I

G

A

V

V

P

P

S

D

G

V

I

Y

G

S

A

A

L

-

C

C

E

K

R

R

L

F

A

E

E

L

G

G

V

V

P

K

V

F

P

V

R

K

P

K

P

P

K

D

P

D

Q

G

K

K

H
x
M

G

K

D

G

N

-

I

-

V

L

A

A

F
|
F

I

I

E

Q

D

D

P

P

D

D

G

G

Y

Y

R

W

I

I

E
|
E

L

I

V

L

Q

N

active site: Q26 (≠ H10), E92 (= E66), H119 (= H85), E165 (= E134)
binding 1-hydroxy-6-[1-(3-methoxypropyl)-1H-pyrrolo[2,3-b]pyridin-5-yl]-4-phenylpyridin-2(1H)-one: Q26 (≠ H10), C53 (≠ R37), L62 (≠ Q46), E92 (= E66), H119 (= H85), M150 (≠ H117), F155 (= F124), E165 (= E134)
binding zinc ion: Q26 (≠ H10), E92 (= E66), H119 (= H85), E165 (= E134)

Sites not aligning to the query:

binding 1-hydroxy-6-[1-(3-methoxypropyl)-1H-pyrrolo[2,3-b]pyridin-5-yl]-4-phenylpyridin-2(1H)-one: 172, 176

1qipA Human glyoxalase i complexed with s-p- nitrobenzyloxycarbonylglutathione (see paper)
36% identity, 94% coverage: 5:137/141 of query aligns to 21:168/176 of 1qipA

query
sites
1qipA

D

D

I

F

K

L

L

L

L

Q

H
x
Q

A

T

M

M

L

L

R
|
R

V

V

S

K

D

D

M

P

E

K

R

K

S

S

L

L

D

D

F

F

Y

Y

T

T

R

R

L

V

L

L

G

G

M

M

R

T

V

L

I

I

Q
|
Q

Q

K

R
x
C

E

D

H

F

K

P

K

I

N

M

Q

K

F
|
F

T

S

Q

L

A

Y

Y
x
F

V

L

G

A

Y

Y

G

-

T

-

A

-

E

E

D

D

P

K

L

N

D

D

S

I

P

P

-

K

-

E

-

K

-

D

-

E

-

K

-
x
I

-

A

-

W

-

A

-
x
L

-

S

-

R

A

K

M

A

V

T

I

L

E
|
E

L

L

V
x
T

A

H

N
|
N

W

W

T

G

Q

T

E

E

-

D

-

E

-

T

V

Q

P

S

Y

Y

S

H

Q

N

G

G

S

N

A

S

-

D

-

P

-
x
R

-

G

F

F

G

G

H
|
H

I

I

A

G

I

I

G

A

V

V

P

P

S

D

G

V

I

Y

G

S

A

A

L

-

C

C

E

K

R

R

L

F

A

E

E

L

G

G

V

V

P

K

V

F

P

V

R

K

P

K

P

P

K

D

P

D

Q

G

K

K

H
x
M

G

K

D

G

N

-

I

-

V

L

A

A

F
|
F

I

I

E

Q

D

D

P

P

D

D

G

G

Y

Y

R

W

I

I

E
|
E

L

I

V

L

Q

N

active site: Q26 (≠ H10), E92 (= E66), H119 (= H85), E165 (= E134)
binding s-p-nitrobenzyloxycarbonylglutathione: R30 (= R14), Q51 (= Q35), C53 (≠ R37), F60 (= F44), F64 (≠ Y48), I81 (vs. gap), L85 (vs. gap), T94 (≠ V68), N96 (= N70), R115 (vs. gap), M150 (≠ H117), F155 (= F124)
binding zinc ion: Q26 (≠ H10), E92 (= E66), H119 (= H85), E165 (= E134)

Sites not aligning to the query:

binding s-p-nitrobenzyloxycarbonylglutathione: 172

1qinA Human glyoxalase i complexed with s-(n-hydroxy-n-p- iodophenylcarbamoyl) glutathione (see paper)
36% identity, 94% coverage: 5:137/141 of query aligns to 21:168/176 of 1qinA

query
sites
1qinA

D

D

I

F

K

L

L

L

L

Q

H
x
Q

A

T

M

M

L

L

R
|
R

V

V

S

K

D

D

M

P

E

K

R

K

S

S

L

L

D

D

F

F

Y

Y

T

T

R

R

L

V

L

L

G

G

M

M

R

T

V

L

I

I

Q

Q

Q

K

R

C

E

D

H

F

K

P

K

I

N

M

Q

K

F
|
F

T

S

Q
x
L

A

Y

Y
x
F

V

L

G

A

Y

Y

G

-

T

-

A

-

E

E

D

D

P

K

L

N

D

D

S

I

P

P

-

K

-

E

-

K

-

D

-

E

-

K

-

I

-

A

-

W

-

A

-

L

-

S

-

R

A

K

M

A

V

T

I

L

E
|
E

L

L

V
x
T

A

H

N
|
N

W

W

T

G

Q

T

E

E

-

D

-

E

-

T

V

Q

P

S

Y

Y

S

H

Q

N

G

G

S

N

A

S

-

D

-

P

-
x
R

-

G

F

F

G

G

H
|
H

I

I

A

G

I

I

G

A

V

V

P

P

S

D

G

V

I

Y

G

S

A

A

L

-

C

C

E

K

R

R

L

F

A

E

E

L

G

G

V

V

P

K

V

F

P

V

R
x
K

P

K

P

P

K

D

P

D

Q
x
G

K
|
K

H
x
M

G

K

D

G

N

-

I

-

V

L

A

A

F

F

I

I

E

Q

D

D

P

P

D

D

G

G

Y

Y

R

W

I

I

E
|
E

L

I

V

L

Q

N

active site: Q26 (≠ H10), E92 (= E66), H119 (= H85), E165 (= E134)
binding s-(n-hydroxy-n-iodophenylcarbamoyl)glutathione: Q26 (≠ H10), R30 (= R14), F60 (= F44), L62 (≠ Q46), F64 (≠ Y48), E92 (= E66), T94 (≠ V68), N96 (= N70), R115 (vs. gap), H119 (= H85), K143 (≠ R110), G148 (≠ Q115), K149 (= K116), M150 (≠ H117), E165 (= E134)
binding zinc ion: Q26 (≠ H10), E92 (= E66)

Sites not aligning to the query:

binding s-(n-hydroxy-n-iodophenylcarbamoyl)glutathione: 172

1froA Human glyoxalase i with benzyl-glutathione inhibitor (see paper)
36% identity, 94% coverage: 5:137/141 of query aligns to 21:168/176 of 1froA

query
sites
1froA

D

D

I

F

K

L

L

L

L

Q

H
x
Q

A

T

M

M

L

L

R
|
R

V

V

S

K

D

D

M

P

E

K

R

K

S

S

L

L

D

D

F

F

Y

Y

T

T

R

R

L

V

L

L

G

G

M

M

R

T

V

L

I

I

Q

Q

Q

K

R

C

E

D

H

F

K

P

K

I

N

M

Q

K

F
|
F

T

S

Q

L

A

Y

Y

F

V

L

G

A

Y

Y

G

-

T

-

A

-

E

E

D

D

P

K

L

N

D

D

S

I

P

P

-

K

-

E

-

K

-

D

-

E

-

K

-

I

-

A

-

W

-

A

-

L

-

S

-

R

A

K

M

A

V

T

I

L

E
|
E

L

L

V
x
T

A

H

N
|
N

W

W

T

G

Q

T

E

E

-

D

-

E

-

T

V

Q

P

S

Y

Y

S

H

Q

N

G

G

S

N

A

S

-

D

-

P

-
x
R

-

G

F

F

G

G

H
|
H

I

I

A

G

I

I

G

A

V

V

P

P

S

D

G

V

I

Y

G

S

A

A

L

-

C

C

E

K

R

R

L

F

A

E

E

L

G

G

V

V

P

K

V

F

P

V

R

K

P

K

P

P

K

D

P

D

Q

G

K

K

H
x
M

G

K

D

G

N

-

I

-

V

L

A

A

F
|
F

I

I

E

Q

D

D

P

P

D

D

G

G

Y

Y

R

W

I

I

E
|
E

L

I

V

L

Q

N

active site: Q26 (≠ H10), E92 (= E66), H119 (= H85), E165 (= E134)
binding s-benzyl-glutathione: R30 (= R14), F60 (= F44), T94 (≠ V68), N96 (= N70), R115 (vs. gap), M150 (≠ H117), F155 (= F124), E165 (= E134)
binding zinc ion: Q26 (≠ H10), E92 (= E66), H119 (= H85), E165 (= E134)

Sites not aligning to the query:

binding s-benzyl-glutathione: 176

6l0uB Crystal structure of mouse glyoxalase i complexed with a small molecule inhibitor
35% identity, 94% coverage: 5:137/141 of query aligns to 22:169/177 of 6l0uB

query
sites
6l0uB

D

D

I

F

K

L

L

L

L

Q

H
x
Q

A

T

M

M

L

L

R

R

V

I

S

K

D

D

M

P

E

K

R

K

S

S

L

L

D

D

F

F

Y

Y

T

T

R

R

L

V

L

L

G

G

M

L

R

T

V

L

I

L

Q

Q

Q

K

R

L

E

D

H

F

K

P

K

A

N

M

Q

K

F

F

T

S

Q

L

A

Y

Y

F

V

L

G

A

Y

Y

G

E

T

D

A

K

E

N

D

D

-

I

P

P

L

K

D

D

-

K

-

S

-

E

-

K

-

T

-

A

-

W

-

T

-

F

S

S

P

R

A

K

M

A

V

T

I

L

E
|
E

L

L

V

T

A

H

N

N

W

W

T

G

Q

T

E

E

-

D

-

E

-

T

V

Q

P

S

Y

Y

S

H

Q

N

G

G

S

N

A

S

-

D

-

P

-
x
R

-

G

F

F

G

G

H
|
H

I

I

A

G

I

I

G

A

V

V

P

P

S

D

G

V

I

Y

G

S

A

A

L

-

C

C

E

K

R

R

L

F

A

E

E

L

G

G

V

V

P

K

V

F

P

V

R
x
K

P

K

P

P

K

D

P
x
D

Q
x
G

K

K

H

M

G

K

D

G

N

-

I

-

V

L

A

A

F

F

I

I

E

Q

D

D

P

P

D

D

G

G

Y

Y

R
x
W

I

I

E
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E

L

I

V

L

Q

N

active site: Q27 (≠ H10), E93 (= E66), H120 (= H85), E166 (= E134)
binding N-[4-(trifluoromethyloxy)phenyl]-1,3,4,9-tetrahydropyrido[3,4-b]indole-2-carbothioamide: R116 (vs. gap), K144 (≠ R110), D148 (≠ P114), G149 (≠ Q115), W164 (≠ R132)
binding zinc ion: Q27 (≠ H10), E93 (= E66), H120 (= H85), E166 (= E134)

4kykB Crystal structure of mouse glyoxalase i complexed with indomethacin (see paper)
35% identity, 94% coverage: 5:137/141 of query aligns to 22:169/177 of 4kykB

query
sites
4kykB

D

D

I

F

K

L

L

L

L

Q

H
x
Q

A

T

M

M

L

L

R

R

V

I

S

K

D

D

M

P

E

K

R

K

S

S

L

L

D

D

F

F

Y

Y

T

T

R

R

L

V

L

L

G

G

M

L

R

T

V

L

I

L

Q

Q

Q

K

R

L

E

D

H

F

K

P

K

A

N

M

Q

K

F

F

T

S

Q

L

A

Y

Y

F

V

L

G

A

Y

Y

G

E

T

D

A

K

E

N

D

D

-

I

P

P

L

K

D

D

-

K

-

S

-

E

-

K

-

T

-

A

-

W

-

T

-

F

S

S

P

R

A

K

M

A

V

T

I

L

E
|
E

L

L

V

T

A

H

N

N

W

W

T

G

Q

T

E

E

-

D

-

E

-

T

V

Q

P

S

Y

Y

S

H

Q

N

G

G

S

N

A

S

-

D

-

P

-

R

-

G

F

F

G

G

H
|
H

I

I

A

G

I

I

G

A

V

V

P

P

S

D

G

V

I

Y

G

S

A

A

L

-

C

C

E

K

R

R

L

F

A

E

E

L

G

G

V

V

P

K

V

F

P

V

R
x
K

P

K

P

P

K

D

P

D

Q

G

K
|
K

H
x
M

G

K

D

G

N

-

I

-

V
x
L

A

A

F
|
F

I

I

E

Q

D

D

P

P

D

D

G

G

Y

Y

R

W

I

I

E
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E

L

I

V

L

Q

N

active site: Q27 (≠ H10), E93 (= E66), H120 (= H85), E166 (= E134)
binding indomethacin: K144 (≠ R110), K150 (= K116), M151 (≠ H117), L154 (≠ V122), F156 (= F124)
binding zinc ion: Q27 (≠ H10), E93 (= E66), H120 (= H85), E166 (= E134)

7wszA Human glyoxalase i (with c-ter his tag) in glycerol-bound form (see paper)
36% identity, 94% coverage: 5:137/141 of query aligns to 21:168/183 of 7wszA

query
sites
7wszA

D

D

I

F

K

L

L

L

L

Q

H
x
Q

A

T

M

M

L

L

R

R

V

V

S

K

D

D

M

P

E

K

R

K

S

S

L

L

D

D

F

F

Y

Y

T

T

R

R

L

V

L

L

G

G

M

M

R

T

V

L

I
|
I

Q
|
Q

Q
x
K

R

C

E

D

H

F

K

P

K

I

N

M

Q

K

F

F

T

S

Q

L

A

Y

Y

F

V

L

G

A

Y

Y

G

-

T

-

A

-

E

E

D

D

P

K

L

N

D

D

S

I

P

P

-

K

-

E

-

K

-

D

-

E

-
x
K

-

I

-

A

-

W

-

A

-

L

-

S

-

R

A

K

M

A

V

T

I

L

E
|
E

L

L

V

T

A

H

N

N

W

W

T

G

Q

T

E

E

-

D

-

E

-

T

V

Q

P

S

Y

Y

S

H

Q

N

G

G

S

N

A

S

-

D

-

P

-

R

-

G

F

F

G

G

H
|
H

I

I

A

G

I

I

G

A

V

V

P

P

S

D

G

V

I

Y

G

S

A

A

L

-

C

C

E

K

R

R

L

F

A

E

E

L

G

G

V

V

P

K

V

F

P

V

R

K

P

K

P

P

K

D

P

D

Q

G

K

K

H
x
M

G

K

D

G

N

-

I

-

V

L

A

A

F

F

I

I

E

Q

D

D

P

P

D

D

G

G

Y

Y

R

W

I

I

E
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E

L

I

V

L

Q

N

binding glycerol: I50 (= I34), Q51 (= Q35), K52 (≠ Q36), K80 (vs. gap), H119 (= H85), M150 (≠ H117), E165 (= E134)
binding zinc ion: Q26 (≠ H10), E92 (= E66), H119 (= H85), E165 (= E134)

Sites not aligning to the query:

binding glycerol: 176

4pv5A Crystal structure of mouse glyoxalase i in complexed with 18-beta- glycyrrhetinic acid (see paper)
35% identity, 94% coverage: 5:137/141 of query aligns to 17:164/170 of 4pv5A

query
sites
4pv5A

D

D

I

F

K

L

L

L

L

Q

H
x
Q

A

T

M

M

L

L

R

R

V

I

S

K

D

D

M

P

E

K

R

K

S

S

L

L

D

D

F

F

Y

Y

T

T

R

R

L

V

L

L

G

G

M

L

R

T

V

L

I

L

Q

Q

Q

K

R

L

E

D

H

F

K

P

K

A

N

M

Q

K

F

F

T

S

Q

L

A

Y

Y

F

V

L

G

A

Y

Y

G

E

T

D

A

K

E

N

D

D

-

I

P

P

L

K

D

D

-

K

-

S

-

E

-

K

-

T

-

A

-

W

-

T

-

F

S

S

P

R

A

K

M

A

V

T

I

L

E
|
E

L

L

V

T

A

H

N

N

W

W

T

G

Q

T

E

E

-

D

-

E

-

T

V

Q

P

S

Y

Y

S

H

Q

N

G

G

S

N

A

S

-

D

-

P

-
x
R

-

G

F

F

G

G

H
|
H

I

I

A

G

I

I

G

A

V

V

P

P

S

D

G

V

I

Y

G

S

A

A

L

-

C

C

E

K

R

R

L

F

A

E

E

L

G

G

V

V

P

K

V

F

P

V

R
x
K

P

K

P

P

K

D

P

D

Q

G

K

K

H

M

G

K

D

G

N

-

I

-

V

L

A

A

F

F

I

I

E

Q

D

D

P

P

D

D

G

G

Y

Y

R

W

I

I

E
|
E

L

I

V

L

Q

N

active site: Q22 (≠ H10), E88 (= E66), H115 (= H85), E161 (= E134)
binding (3beta,5beta,14beta)-3-hydroxy-11-oxoolean-12-en-29-oic acid: R111 (vs. gap), K139 (≠ R110)
binding zinc ion: Q22 (≠ H10), E88 (= E66)

4kykA Crystal structure of mouse glyoxalase i complexed with indomethacin (see paper)
35% identity, 94% coverage: 5:137/141 of query aligns to 24:171/179 of 4kykA

query
sites
4kykA

D

D

I

F

K

L

L

L

L

Q

H
x
Q

A

T

M

M

L

L

R

R

V

I

S

K

D

D

M

P

E

K

R

K

S

S

L

L

D

D

F

F

Y

Y

T

T

R

R

L

V

L

L

G

G

M

L

R

T

V

L

I

L

Q

Q

Q

K

R

L

E

D

H

F

K

P

K

A

N

M

Q

K

F

F

T

S

Q

L

A

Y

Y

F

V

L

G

A

Y

Y

G

E

T

D

A

K

E

N

D

D

-

I

P

P

L

K

D

D

-

K

-

S

-

E

-

K

-

T

-

A

-

W

-

T

-

F

S

S

P

R

A

K

M

A

V

T

I

L

E
|
E

L

L

V

T

A

H

N

N

W

W

T

G

Q

T

E

E

-

D

-

E

-

T

V

Q

P

S

Y

Y

S

H

Q

N

G

G

S

N

A

S

-

D

-

P

-

R

-

G

F

F

G

G

H
|
H

I

I

A

G

I

I

G

A

V

V

P

P

S

D

G

V

I

Y

G

S

A

A

L

-

C

C

E

K

R

R

L

F

A

E

E

L

G

G

V

V

P

K

V

F

P

V

R

K

P

K

P

P

K

D

P

D

Q

G

K

K

H

M

G

K

D

G

N

-

I

-

V

L

A

A

F

F

I

I

E

Q

D

D

P

P

D

D

G

G

Y

Y

R

W

I

I

E
|
E

L

I

V

L

Q

N

active site: Q29 (≠ H10), E95 (= E66), H122 (= H85), E168 (= E134)
binding zinc ion: Q29 (≠ H10), E95 (= E66), H122 (= H85), E168 (= E134)

Query Sequence

>AZOBR_RS29310 FitnessBrowser__azobra:AZOBR_RS29310
MTGTDIKLLHAMLRVSDMERSLDFYTRLLGMRVIQQREHKKNQFTQAYVGYGTAEDPLDS
PAMVIELVANWTQEVPYSQGSAFGHIAIGVPSGIGALCERLAAEGVPVPRPPKPQKHGDN
IVAFIEDPDGYRIELVQRAAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory