SitesBLAST

P19920 Carbon monoxide dehydrogenase medium chain; CO dehydrogenase subunit M; CO-DH M; EC 1.2.5.3 from Afipia carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5) (Oligotropha carboxidovorans) (see 2 papers)
33% identity, 96% coverage: 6:265/270 of query aligns to 6:279/288 of P19920

query
sites
P19920

F

F

D

D

Y

Y

A

H

Q

R

P

P

A

K

T

S

L

I

D

A

A

D

A

A

L

V

E

A

L

L

S

T

R

K

E

L

D

G

V

E

M

D

V

A

R

R

P

P

V

L

A
|
A

G
|
G

S
x
G

Q
x
H

S
|
S

L

L

G

I

P

P

M

I

L

M

N

K

L

T

R

R

L

L

A

A

Q

T

P

P

E

E

L

H

L

L

V

V

D

D

I

L

T

R

R

D

I

I

A

G

E

D

L

L

R

V

T

G

I

I

R

R

R

E

E

E

G

G

D

T

R

D

L

V

V

V

I

I

G

G

A

A

C

M

V

T

T

T

H

Q

A

H

R

A

L

L

E

I

D

G

G

S

D

D

Y

F

P

L

D

A

V

A

T

K

R

L

G

P

V

I

L

I

P

R

S

E

V

T

A

S

A

L

V

L

I

I

A

A

Y

D

R

P

A

Q

V

I

R

R

N

Y

R

M

G

G

T
|
T

I
|
I

G
|
G

S
x
N

L

A

A

A

H

N

A

G

D

D

P

P

A

G

A

N

D

D

W

M

V

P

N

A

V

L

L

M

T

Q

A

C

L

L

G

G

A

A

D

A

V

Y

V

E

I

L

V

T

G

G

Q

P

G

E

G

G

R

A

R

R

S

I

V

V

P

A

M

A

T

R

D

D

F

Y

I

Y

L

Q

G

G

V

A

F

Y

E

F

T

T

A

A

L

I

Q

E

P

P

G

G

E

E

I

L

V

L

A

T

E

A

I

I

H

R

V

I

P

P

A

V

L

P

S

P

E

T

R

G

A

H

R

G

W

Y

G

A

Y

Y

Y

E

K

K

V

L

C

K

R

R

K

K

T

I

G

G

E

D

F

Y

S

A

H

T

A

A

T

A

G

A

A

A

V

V

L

V

I

L

D

T

P

M

A

S

R

G

G

G

V

-

Q

-

R

K

C

C

V

V

A

T

G

A

A

S

-

I

-

G

-

L

-

T

-

N

T

V

S

A

G

N

K

T

P

P

V

L

-

W

-

A

-

E

-

A

-

G

-

K

V

V

I

L

D

V

G

G

P

T

A

A

L

L

F

D

T

K

D

P

G

A

C

L

T

D

E

K

A

A

A

V

L

A

A

L

A

A

H

E

L

A

A

I

G

T

S

A

P

P

A

A

A

S

D

D

-

G

-

R

D

G

P

P

I

A

A

E

L

Y

R

R

T

T

H

K

T

M

-

A

-

G

V

V

A

M

L

L

K

R

R

R

A

A

I

V

AGGHS 32:36 (≠ AGSQS 32:36) binding FAD
TIGGN 111:115 (≠ TIGGS 111:115) binding FAD

1n5wC Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
33% identity, 96% coverage: 6:265/270 of query aligns to 6:279/287 of 1n5wC

query
sites
1n5wC

F

F

D

D

Y

Y

A

H

Q

R

P

P

A

K

T

S

L

I

D

A

A

D

A

A

L

V

E

A

L

L

S

T

R

K

E

L

D

G

V

E

M

D

V

A

R

R

P

P

V

L

A

A

G

G

S

G

Q
x
H

S

S

L

L

G

I

P

P

M

I

L

M

N

K

L

T

R

R

L

L

A

A

Q

T

P

P

E

E

L

H

L

L

V

V

D

D

I

L

T

R

R

D

I

I

A

G

E

D

L

L

R

V

T

G

I

I

R

R

R

E

E

E

G

G

D

T

R

D

L

V

V

V

I

I

G

G

A

A

C

M

V

T

T

T

H

Q

A

H

R

A

L

L

E

I

D

G

G

S

D

D

Y

F

P

L

D

A

V

A

T

K

R

L

G

P

V

I

L

I

P

R

S

E

V

T

A

S

A

L

V

L

I
|
I

A
|
A

Y

D

R

P

A

Q

V

I

R

R

N

Y

R

M

G

G

T

T

I

I

G

G

S
x
N

L

A

A

A

H

N

A

G

D

D

P

P

A

G

A
x
N

D
|
D

W

M

V

P

N

A

V

L

L

M

T

Q

A

C

L

L

G

G

A

A

D

A

V

Y

V

E

I

L

V

T

G

G

Q

P

G

E

G

G

R

A

R

R

S

I

V

V

P

A

M

A

T

R

D

D

F

Y

I

Y

L

Q

G

G

V

A

F

Y

E

F

T

T

A

A

L

I

Q

E

P

P

G

G

E

E

I

L

V

L

A

T

E

A

I

I

H

R

V

I

P

P

A

V

L

P

S

P

E

T

R

G

A

H

R

G

W

Y

G

A

Y

Y

Y

E

K

K

V

L

C

K

R

R

K

K

T

I

G

G

E

D

F
x
Y

S

A

H

T

A

A

T

A

G

A

A

A

V

V

L

V

I

L

D

T

P

M

A

S

R

G

G

G

V

-

Q

-

R

K

C

C

V

V

A

T

G

A

A

S

-

I

-

G

-

L

-

T

-

N

T

V

S

A

G

N

K

T

P

P

V

L

-

W

-

A

-

E

-

A

-

G

-

K

V

V

I

L

D

V

G

G

P

T

A

A

L

L

F

D

T

K

D

P

G

A

C

L

T

D

E

K

A

A

A

V

L

A

A

L

A

A

H

E

L

A

A

I

G

T

S

A

P

P

A

A

A

S

D

D

-

G

-

R

D

G

P

P

I

A

A

E

L

Y

R

R

T

T

H

K

T

M

-

A

-

G

V

V

A

M

L

L

K

R

R

R

A

A

I

V

binding flavin-adenine dinucleotide: H35 (≠ Q35), I101 (= I101), A102 (= A102), N115 (≠ S115), N123 (≠ A123), D124 (= D124), Y193 (≠ F193)

Q0QLF4 Nicotinate dehydrogenase FAD-subunit; NDH; Nicotinic acid hydroxylase FAD-subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see paper)
32% identity, 69% coverage: 5:189/270 of query aligns to 3:191/296 of Q0QLF4

query
sites
Q0QLF4

D

D

F

F

D

E

Y

F

A

F

Q

A

P

P

A

K

T

T

L

L

D

E

A

E

A

A

L

K

E

G

L

L

S

H

R

Q

-

Y

E

K

D

D

V

V

M

P

V

P

R

A

P
x
I

V
x
I

A
|
A

G
|
G

S
x
G

Q
x
T

S
x
D

L
|
L

G

V

P

I

M

E

L

I

N

N

L

D

R

R

L

W

A

E

Q

K

P

P

E

D

L

V

V

I

D

D

I

I

T

K

R

K

I

L

A

K

E

E

L

L

R

E

T

Y

I

I

R

R

R

V

E

E

G

E

D

N

R

T

L

I

V

H

I

I

G

G

A

A

C

L

V

S

T

T

H

F

A

T

R

Q

L

I

E

E

D

N

G

H

D

P

Y

F

P

I

D

R

V

S

T

H

R

V

G

R

V

A

L

L

P

Y

S

K

V

A

A

A

A

S

V

Q

I

V

A
x
G

Y

S

R

P

A

Q

V

I

R

R

N

N

R

L

G

G

T
|
T

I
|
I

G
|
G

S
x
N

L

L

A

S

H

T

A

S

D

S

P

V

A

A

A

G

D
|
D

W

G

V

V

N

S

V

A

L

M

T

T

A

T

L

L

G

D

A

A

D

T

V

V

I

L

V

E

G

S

Q

V

G

R

G

G

R

T

R

R

S

Q

V

M

P

K

M

L

T

T

D

D

F

F

I

F

L

D

G

G

V

E

-

G

F

F

E

K

-

R

-
x
R

T

N

A

A

L

L

Q

E

P

A

G

D

E

E

I

I

V
x
M

A

T

E

E

I

V

H

I

V

I

P

D

A

R

L

P

S

D

E

A

R

H

A

S

R

A

W

S

G

A

Y

F

Y

Y

K
|
K

V

L

C

A

R

K

K

R

29:36 (vs. 30:37, 38% identical) binding FAD
G101 (≠ A102) binding FAD
TIGGN 110:114 (≠ TIGGS 111:115) binding FAD
D123 (= D124) binding FAD
R160 (vs. gap) binding FAD
M169 (≠ V167) binding FAD
K187 (= K185) binding FAD

3hrdG Crystal structure of nicotinate dehydrogenase (see paper)
32% identity, 69% coverage: 5:189/270 of query aligns to 3:191/292 of 3hrdG

query
sites
3hrdG

D

D

F

F

D

E

Y

F

A

F

Q

A

P

P

A

K

T

T

L

L

D

E

A

E

A

A

L

K

E

G

L

L

S

H

R

Q

-

Y

E

K

D

D

V

V

M

P

V

P

R

A

P
x
I

V

I

A
|
A

G
|
G

S
x
G

Q
x
T

S
x
D

L

L

G

V

P

I

M

E

L

I

N

N

L

D

R

R

L

W

A

E

Q

K

P

P

E

D

L

V

V

I

D

D

I

I

T

K

R

K

I

L

A

K

E

E

L

L

R

E

T

Y

I

I

R

R

R

V

E

E

G

E

D

N

R

T

L

I

V

H

I

I

G

G

A

A

C

L

V

S

T

T

H
x
F

A

T

R

Q

L

I

E

E

D

N

G

H

D

P

Y

F

P

I

D

R

V

S

T

H

R

V

G

R

V

A

L

L

P

Y

S

K

V

A

A

A

A

S

V

Q

I
x
V

A
x
G

Y

S

R

P

A

Q

V
x
I

R

R

N

N

R

L

G

G

T
|
T

I

I

G

G

G
|
G

S
x
N

L

L

A
x
S

H
x
T

A

S

D

S

P

V

A

A

A
x
G

D
|
D

W

G

V

V

N

S

V

A

L

M

T

T

A

T

L

L

G

D

A

A

D

T

V

V

I

L

V

E

G

S

Q

V

G

R

G

G

R

T

R

R

S

Q

V

M

P

K

M

L

T

T

D

D

F

F

I

F

L

D

G

G

V

E

-

G

F

F

E

K

-

R

T

N

A

A

L

L

Q

E

P

A

G

D

E

E

I
|
I

V
x
M

A

T

E

E

I

V

H

I

V

I

P

D

A

R

L

P

S

D

E

A

R

H

A

S

R

A

W

S

G

A

Y

F

Y

Y

K

K

V

L

C

A

R

K

K

R

binding flavin-adenine dinucleotide: I29 (≠ P30), A31 (= A32), G32 (= G33), G33 (≠ S34), T34 (≠ Q35), D35 (≠ S36), F77 (≠ H78), V100 (≠ I101), G101 (≠ A102), I105 (≠ V106), T110 (= T111), G113 (= G114), N114 (≠ S115), S116 (≠ A117), T117 (≠ H118), G122 (≠ A123), D123 (= D124), I168 (= I166), M169 (≠ V167)

2e3tA Crystal structure of rat xanthine oxidoreductase mutant (w335a and f336l) (see paper)
26% identity, 73% coverage: 8:205/270 of query aligns to 208:417/1291 of 2e3tA

query
sites
2e3tA

Y

W

A

I

Q

Q

P

A

A

S

T

T

L

M

D

E

A

E

A

L

L

L

E

D

L

L

L

K

S

A

R

Q

E

H

D

P

V

-

M

D

V

A

R
x
K

P
x
L

V
|
V

A
x
V

G
|
G

S
x
N

Q
x
T

S
x
E

L
x
I

G

G

P

I

M

E

L

M

N

K

L

F

R

K

L

N

A

M

Q

L

P

F

E

P

L

L

L

I

V

V

D

C

I

P

T

A

R

W

I

I

A

P

E

E

L

L

R

N

T

S

I

V

R

V

R

H

E

G

G

P

D

E

R

G

L

I

V

S

I

F

G

G

A

A

C

S

V

C

T

P

H

L

A

S

R

L

L

V

E

E

D

S

-

V

-

L

-

A

-

E

-

I

G

A

D

K

Y

L

P

P

D

E

-

Q

-

K

-

T

-

E

V

V

T

F

R

R

G

G

V

V

L

M

P

E

S

Q

V

L

A

R

A

A

V

-

I
x
L

A

A

Y

G

R

K

A

Q

V

V

R

K

N

S

R

V

G
x
A

T
x
S

I

I

G

G

G
|
G

S
x
N

L

I

A
x
I

H
x
T

A

A

D

S

P

P

A

I

A
x
S

D
|
D

W

L

V

N

N

P

V

V

L

F

T

M

A

A

L

S

G

G

A

A

D

K

V

L

V

T

I

L

V

V

G

S

Q

R

G

G

G

T

R

R

S

T

V

V

P

R

M

M

T

D

-

H

D

T

F

F

I

F

L

P

G

G

V

Y

F

R

E

K

T

T

A

L

L

L

Q

R

P

P

G

E

E

E

I
|
I

V
x
L

A

L

E

S

I

I

H

E

V

I

P

P

A

Y

L

S

S

K

E

E

R

G

A

E

R

F

W

F

G

S

Y

A

Y

F

K

K

-

Q

V

A

C

S

R

R

K

R

T

E

G

D

E

D

F

I

S

A

H

K

A

V

T

T

G

S

A

G

-

M

-

R

V

V

L

L

I

F

D

K

P

P

A

G

binding flavin-adenine dinucleotide: K228 (≠ R29), L229 (≠ P30), V230 (= V31), V231 (≠ A32), G232 (= G33), N233 (≠ S34), T234 (≠ Q35), E235 (≠ S36), I236 (≠ L37), L309 (≠ I101), A318 (≠ G110), S319 (≠ T111), G322 (= G114), N323 (≠ S115), I325 (≠ A117), T326 (≠ H118), S331 (≠ A123), D332 (= D124), I375 (= I166), L376 (≠ V167)

Sites not aligning to the query:

active site: 740, 775, 853, 857, 885, 1233, 1234
binding bicarbonate ion: 812, 813, 850, 882, 883, 887, 888, 891
binding calcium ion: 713, 714, 716, 809, 810, 840, 843, 844, 847, 880, 881
binding flavin-adenine dinucleotide: 44
binding fe2/s2 (inorganic) cluster: 40, 41, 42, 44, 46, 47, 49, 69, 71, 109, 110, 113, 145, 147, 717
binding uric acid: 775, 853, 887, 982, 983, 1051, 1052, 1234

1jroA Crystal structure of xanthine dehydrogenase from rhodobacter capsulatus (see paper)
30% identity, 50% coverage: 54:189/270 of query aligns to 213:344/450 of 1jroA

query
sites
1jroA

I

L

T

S

R

H

I

C

A

K

E

D

L

L

R

A

T

Q

I

I

R

R

R

E

E

T

G

P

D

D

R

G

L

Y

V

G

I

I

G

G

A

A

C

G

V

V

T

T

H

I

A

A

R

A

L

L

E

R

D

A

G

-

D

-

Y

-

P

-

D

-

V

F

T

A

R

E

G

G

V

P

L

H

P

P

S

A

V

L

A

A

A

G

V

L

I

L

-

R

-
x
F

A
|
A

Y

S

R

E

A

Q

V

V

R

R

N

Q

R

V

G

A

T
|
T

I

I

G

G

G
|
G

S
x
N

L

I

A
|
A

H

N

A

G

D

S

P

P

A

I

A
x
G

D
|
D

W

G

V

P

N

P

V

A

L

L

T

I

A

A

L

M

G

G

A

A

D

S

V

L

V

T

I

L

V

R

G

R

Q

G

G

Q

G

E

R

R

S

R

V

M

P

P

M

L

T

E

D

D

F

F

I

F

L

L

G

E

V

Y

F

R

E

K

T

Q

A

D

L
x
R

Q

R

P

P

G

G

E

E

I

F

V
|
V

A

E

E

S

I

V

H

T

V

L

P

P

A

K

L

S

S

A

E

P

R

G

A

L

R

R

W

C

G

-

Y

-

Y

Y

K

K

V

L

C

S

R

K

K

R

binding flavin-adenine dinucleotide: F258 (vs. gap), A259 (= A102), T268 (= T111), G271 (= G114), N272 (≠ S115), A274 (= A117), G280 (≠ A123), D281 (= D124), R318 (≠ L161), V324 (= V167)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 42, 43, 189, 191, 192, 193, 194, 195, 196
binding fe2/s2 (inorganic) cluster: 37, 38, 39, 40, 42, 44, 45, 47, 63, 103, 106, 134, 136

2w54A Crystal structure of xanthine dehydrogenase from rhodobacter capsulatus in complex with bound inhibitor pterin-6-aldehyde (see paper)
30% identity, 50% coverage: 54:189/270 of query aligns to 213:344/450 of 2w54A

query
sites
2w54A

I
x
L

T

S

R

H

I

C

A

K

E

D

L

L

R

A

T

Q

I

I

R

R

R

E

E

T

G

P

D

D

R

G

L

Y

V

G

I

I

G

G

A

A

C

G

V

V

T

T

H

I

A

A

R

A

L

L

E

R

D

A

G

-

D

-

Y

-

P

-

D

-

V

F

T

A

R

E

G

G

V

P

L

H

P

P

S

A

V

L

A

A

A

G

V

L

I

L

-

R

-
x
F

A
|
A

Y

S

R

E

A

Q

V

V

R

R

N

Q

R

V

G
x
A

T
|
T

I

I

G

G

G
|
G

S
x
N

L

I

A
|
A

H
x
N

A

G

D

S

P

P

A

I

A
x
G

D
|
D

W

G

V

P

N

P

V

A

L

L

T

I

A

A

L

M

G

G

A

A

D

S

V

L

V

T

I

L

V

R

G

R

Q

G

G

Q

G

E

R

R

S

R

V

M

P

P

M

L

T

E

D

D

F

F

I

F

L

L

G

E

V

Y

F

R

E

K

T

Q

A

D

L
x
R

Q

R

P

P

G

G

E

E

I

F

V
|
V

A

E

E

S

I

V

H

T

V

L

P

P

A

K

L

S

S

A

E

P

R

G

A

L

R

R

W

-

G

-

Y

C

Y

Y

K

K

V

L

C

S

R

K

K

R

binding flavin-adenine dinucleotide: L213 (≠ I54), F258 (vs. gap), A259 (= A102), A267 (≠ G110), T268 (= T111), G271 (= G114), N272 (≠ S115), A274 (= A117), N275 (≠ H118), G280 (≠ A123), D281 (= D124), R318 (≠ L161), V324 (= V167)

Sites not aligning to the query:

P47989 Xanthine dehydrogenase/oxidase; EC 1.17.1.4; EC 1.17.3.2 from Homo sapiens (Human) (see 4 papers)
25% identity, 73% coverage: 8:205/270 of query aligns to 236:445/1333 of P47989

query
sites
P47989

Y

W

A

I

Q

Q

P

A

A

S

T

T

L

L

D

K

A

E

A

L

L

L

E

D

L

L

L

K

S

A

R

Q

E

H

D

P

V

-

M

D

V

A

R

K

P
x
L

V
|
V

A
x
V

G
|
G

S
x
N

Q
x
T

S
x
E

L
x
I

G

G

P

I

M

E

L

M

N

K

L

F

R

K

L

N

A

M

Q

L

P

F

E

P

L

M

L

I

V

V

D

C

I

P

T

A

R

W

I

I

A

P

E

E

L

L

R

N

T

S

I

V

R

E

R

H

E

G

G

P

D

D

R

G

L

I

V

S

I

F

G

G

A

A

C

A

V

C

T

P

H

L

A

S

R

I

L

V

E

E

D

K

-

T

-

L

-

V

-

D

-

A

-

V

-

A

-

K

-

L

-

P

G

A

D

Q

Y

K

P

T

D

E

V

V

T

F

R

R

G

G

V

V

L

L

P

E

S

Q

V

L

A

R

A

W

V

-

I
x
F

A

A

Y

G

R

K

A

Q

V

V

R

K

N

S

R

V

G

A

T
x
S

I
x
V

G
|
G

S
x
N

L

I

A

I

H

T

A

A

D

S

P

P

A

I

A

S

D
|
D

W

L

V

N

N

P

V

V

L

F

T

M

A

A

L

S

G

G

A

A

D

K

V

L

V

T

I

L

V

V

G

S

Q

R

G

G

G

T

R

R

S

T

V

V

P

Q

M

M

T

D

-

H

D

T

F

F

I

F

L

P

G

G

V

Y

F

R

E
x
K

T

T

A

L

L

L

Q

S

P

P

G

E

E

E

I

I

V

L

A

L

E

S

I

I

H

E

V

I

P

P

A

Y

L

S

S

R

E

E

R

G

A

E

R

Y

W

F

G

S

Y

A

Y

F

K
|
K

-

Q

V

A

C

S

R

R

K

R

T

E

G

D

E

D

F

I

S

A

H

K

A

V

T

T

G

S

A

G

-

M

-

R

V

V

L

L

I

F

D

K

P

P

A

G

257:264 (vs. 30:37, 25% identical) binding FAD
F337 (≠ I101) binding FAD
SVGGN 347:351 (≠ TIGGS 111:115) binding FAD
D360 (= D124) binding FAD
K395 (≠ E158) to M: in dbSNP:rs34929837
K422 (= K185) binding FAD

Sites not aligning to the query:

133 E → K: in dbSNP:rs45447191
172 G → R: in dbSNP:rs45523133
235 T → M: in dbSNP:rs45469499
509 modified: Disulfide link with 1318, In oxidase form
536 modified: Disulfide link with 993, In oxidase form
555 P → S: in dbSNP:rs45577338
584 D → A: in dbSNP:rs45491693
607 R → Q: in dbSNP:rs45442092
617 K → N: in dbSNP:rs45442398
623 T → I: in dbSNP:rs45448694
646 I → V: in dbSNP:rs17323225
703 I → V: in dbSNP:rs17011368
763 L → F: in a breast cancer sample; somatic mutation
791 R → G: in a breast cancer sample; somatic mutation; dbSNP:rs775646772
910 T → M: in dbSNP:rs669884
993 modified: Disulfide link with 536, In oxidase form
1091 V → L: in dbSNP:rs45619033
1109 N → T: in dbSNP:rs45547640
1150 P → R: in dbSNP:rs1042036
1176 R → C: in dbSNP:rs45624433
1296 R → W: in dbSNP:rs45564939
1318 modified: Disulfide link with 509, In oxidase form

2e1qA Crystal structure of human xanthine oxidoreductase mutant, glu803val (see paper)
25% identity, 73% coverage: 8:205/270 of query aligns to 210:419/1307 of 2e1qA

query
sites
2e1qA

Y

W

A

I

Q

Q

P

A

A

S

T

T

L

L

D

K

A

E

A

L

L

L

E

D

L

L

L

K

S

A

R

Q

E

H

D

P

V

-

M

D

V

A

R
x
K

P
x
L

V
|
V

A
x
V

G
|
G

S
x
N

Q
x
T

S
x
E

L
x
I

G

G

P

I

M

E

L

M

N

K

L

F

R

K

L

N

A

M

Q

L

P

F

E

P

L

M

L

I

V

V

D

C

I

P

T

A

R

W

I

I

A

P

E

E

L

L

R

N

T

S

I

V

R

E

R

H

E

G

G

P

D

D

R

G

L

I

V

S

I

F

G

G

A

A

C

A

V

C

T

P

H

L

A

S

R

I

L

V

E

E

D

K

-

T

-

L

-

V

-

D

-

A

-

V

-

A

-

K

-

L

-

P

G

A

D

Q

Y

K

P

T

D

E

V

V

T

F

R

R

G

G

V

V

L

L

P

E

S

Q

V

L

A

R

A

W

V

-

I
x
F

A
|
A

Y

G

R

K

A

Q

V
|
V

R

K

N

S

R

V

G
x
A

T
x
S

I

V

G

G

G
|
G

S
x
N

L

I

A
x
I

H
x
T

A

A

D

S

P

P

A

I

A
x
S

D
|
D

W

L

V

N

N

P

V

V

L

F

T

M

A

A

L

S

G

G

A

A

D

K

V

L

V

T

I

L

V

V

G

S

Q

R

G

G

G

T

R

R

S

T

V

V

P

Q

M

M

T

D

-

H

D

T

F

F

I

F

L

P

G

G

V

Y

F

R

E

K

T

T

A

L

L

L

Q

S

P

P

G

E

E

E

I
|
I

V
x
L

A

L

E

S

I

I

H

E

V

I

P

P

A

Y

L

S

S

R

E

E

R

G

A

E

R

Y

W

F

G

S

Y

A

Y

F

K

K

-

Q

V

A

C

S

R

R

K

R

T

E

G

D

E

D

F

I

S

A

H

K

A

V

T

T

G

S

A

G

-

M

-

R

V

V

L

L

I

F

D

K

P

P

A

G

binding flavin-adenine dinucleotide: K230 (≠ R29), L231 (≠ P30), V232 (= V31), V233 (≠ A32), G234 (= G33), N235 (≠ S34), T236 (≠ Q35), E237 (≠ S36), I238 (≠ L37), F311 (≠ I101), A312 (= A102), V316 (= V106), A320 (≠ G110), S321 (≠ T111), G324 (= G114), N325 (≠ S115), I327 (≠ A117), T328 (≠ H118), S333 (≠ A123), D334 (= D124), I377 (= I166), L378 (≠ V167)

Sites not aligning to the query:

active site: 742, 777, 855, 859, 887, 1235, 1236
binding bicarbonate ion: 814, 815, 852, 886, 889, 890, 893
binding calcium ion: 715, 716, 718, 741, 747, 780, 781, 784, 811, 812
binding flavin-adenine dinucleotide: 43, 44
binding fe2/s2 (inorganic) cluster: 40, 41, 42, 44, 46, 47, 49, 71, 110, 111, 112, 114, 146, 148, 719
binding hydroxy(dioxo)molybdenum: 773, 774, 887, 1053, 1054, 1236

2ckjA Human milk xanthine oxidoreductase
25% identity, 73% coverage: 8:205/270 of query aligns to 207:412/1264 of 2ckjA

query
sites
2ckjA

Y

W

A

I

Q

Q

P

A

A

S

T

T

L

L

D

K

A

E

A

L

L

L

E

D

L

L

L

K

S

A

R

Q

E

H

D

P

V

-

M

D

V

A

R

K

P
x
L

V

V

A
x
V

G
|
G

S
x
N

Q
x
T

S
x
E

L

I

G

G

P

I

M

E

L

M

N

K

L

F

R

K

L

N

A

M

Q

L

P

F

E

P

L

M

L

I

V

V

D

C

I

P

T

A

R

W

I

I

A

P

E

E

L

L

R

N

T

S

I

V

R

E

R

H

E

G

G

P

D

D

R

G

L

I

V

S

I

F

G

G

A

A

C

A

V

C

T

P

H

L

A

S

R

I

L

V

E

E

D

K

-

T

-

L

-

V

-

D

-

A

-

V

-

A

-

K

-

L

-

P

G

A

D

Q

Y

K

P

T

D

E

V

V

T

F

R

R

G

G

V

V

L

L

P

E

S

Q

V

L

A

R

A

W

V

-

I
x
F

A
|
A

Y

G

R

K

A

Q

V

V

R

K

N

S

R

V

G
x
A

T
x
S

I

V

G

G

G
|
G

S
x
N

L

I

A
x
I

H
x
T

A

A

D

S

P

P

A

I

A

S

D
|
D

W

L

V

N

N

P

V

V

L

F

T

M

A

A

L

S

G

G

A

A

D

K

V

L

V

T

I

L

V

V

G

S

Q

R

G

G

G

T

R

R

S

T

V

V

P

Q

M

M

T

D

-

H

D

T

F

F

I

F

L

P

G

G

V

Y

F

R

E

K

T

T

A

L

L

L

Q

S

P

P

G

E

E

E

I

I

V
x
L

A

L

E

S

I

I

H

E

V

I

P

P

A

Y

L

S

S

R

E

E

R

G

A

E

R

-

W

-

G

-

Y

Y

Y

F

K

S

V

A

C

F

R

K

K

Q

T

A

G

S

E

D

F

I

S

A

H

K

A

V

T

T

G

S

A

G

-

M

-

R

V

V

L

L

I

F

D

K

P

P

A

G

binding flavin-adenine dinucleotide: L228 (≠ P30), V230 (≠ A32), G231 (= G33), N232 (≠ S34), T233 (≠ Q35), E234 (≠ S36), F308 (≠ I101), A309 (= A102), A317 (≠ G110), S318 (≠ T111), G321 (= G114), N322 (≠ S115), I324 (≠ A117), T325 (≠ H118), D331 (= D124), L375 (≠ V167)

Sites not aligning to the query:

active site: 711, 746, 824, 828, 856, 1204, 1205
binding fe2/s2 (inorganic) cluster: 40, 41, 42, 44, 46, 47, 49, 71, 111, 112, 114, 146, 147, 148

6a7xA Rat xanthine oxidoreductase, d428a variant, NAD bound form
25% identity, 73% coverage: 8:205/270 of query aligns to 208:417/1295 of 6a7xA

query
sites
6a7xA

Y

W

A

I

Q

Q

P

A

A

S

T

T

L

M

D

E

A

E

A

L

L

L

E

D

L

L

L

K

S

A

R

Q

E

H

D

P

V

-

M

D

V

A

R
x
K

P
x
L

V
|
V

A
x
V

G
|
G

S
x
N

Q
x
T

S
x
E

L
x
I

G

G

P

I

M

E

L

M

N

K

L

F

R

K

L

N

A

M

Q

L

P

F

E

P

L

L

L

I

V

V

D

C

I

P

T

A

R

W

I

I

A

P

E

E

L

L

R

N

T

S

I

V

R

V

R

H

E

G

G

P

D

E

R

G

L

I

V

S

I

F

G

G

A

A

C

S

V

C

T

P

H

L

A

S

R

L

L

V

E

E

D

S

-

V

-

L

-

A

-

E

-

I

G

A

D

K

Y

L

P

P

D

E

-

Q

-

K

-

T

-

E

V

V

T

F

R

R

G

G

V

V

L

M

P

E

S

Q

V

L

A

R

A

W

V

-

I
x
F

A

A

Y

G

R

K

A

Q

V
|
V

R

K

N

S

R

V

G

A

T
x
S

I

I

G

G

G
|
G

S
x
N

L

I

A
x
I

H
x
T

A

A

D

S

P
|
P

A

I

A

S

D
|
D

W

L

V

N

N

P

V

V

L

F

T

M

A

A

L

S

G

G

A

A

D

K

V

L

V

T

I

L

V

V

G

S

Q

R

G

G

G

T

R

R

S

T

V

V

P

R

M

M

T

D

-

H

D

T

F

F

I

F

L

P

G

G

V
x
Y

F
x
R

E

K

T

T

A

L

L

L

Q

R

P

P

G

E

E

E

I

I

V
x
L

A

L

E

S

I

I

H

E

V

I

P

P

A

Y

L

S

S

K

E

E

R

G

A

E

R

F

W

F

G

S

Y

A

Y

F

K

K

-

Q

V

A

C

S

R

R

K

R

T

E

G

A

E

D

F

I

S

A

H

K

A

V

T

T

G

S

A

G

-

M

-

R

V

V

L

L

I

F

D

K

P

P

A

G

binding flavin-adenine dinucleotide: K228 (≠ R29), L229 (≠ P30), V230 (= V31), V231 (≠ A32), G232 (= G33), N233 (≠ S34), T234 (≠ Q35), E235 (≠ S36), I236 (≠ L37), F309 (≠ I101), V314 (= V106), S319 (≠ T111), G322 (= G114), N323 (≠ S115), I325 (≠ A117), T326 (≠ H118), D332 (= D124), L376 (≠ V167)
binding nicotinamide-adenine-dinucleotide: P329 (= P121), Y365 (≠ V156), R366 (≠ F157)

Sites not aligning to the query:

active site: 740, 775, 853, 857, 885, 1233, 1234
binding bicarbonate ion: 812, 813, 850, 883, 884, 887, 888, 891
binding flavin-adenine dinucleotide: 44
binding fe2/s2 (inorganic) cluster: 40, 41, 42, 44, 46, 47, 49, 69, 71, 109, 110, 111, 113, 145, 147
binding nicotinamide-adenine-dinucleotide: 432, 433, 473, 480
binding uric acid: 775, 853, 887, 982, 983, 1052, 1234

6a7xB Rat xanthine oxidoreductase, d428a variant, NAD bound form
25% identity, 73% coverage: 8:205/270 of query aligns to 206:415/1291 of 6a7xB

query
sites
6a7xB

Y

W

A

I

Q

Q

P

A

A

S

T

T

L

M

D

E

A

E

A

L

L

L

E

D

L

L

L

K

S

A

R

Q

E

H

D

P

V

-

M

D

V

A

R

K

P
x
L

V
|
V

A
x
V

G
|
G

S
x
N

Q
x
T

S
x
E

L
x
I

G

G

P

I

M

E

L

M

N

K

L

F

R

K

L

N

A

M

Q

L

P

F

E

P

L

L

L

I

V

V

D

C

I

P

T

A

R

W

I

I

A

P

E

E

L

L

R

N

T

S

I

V

R

V

R

H

E

G

G

P

D

E

R

G

L

I

V

S

I

F

G

G

A

A

C

S

V

C

T

P

H

L

A

S

R

L

L

V

E

E

D

S

-

V

-

L

-

A

-

E

-

I

G

A

D

K

Y

L

P

P

D

E

-

Q

-

K

-

T

-

E

V

V

T

F

R

R

G

G

V

V

L

M

P

E

S

Q

V

L

A

R

A

W

V

-

I
x
F

A

A

Y

G

R

K

A

Q

V
|
V

R

K

N

S

R

V

G

A

T
x
S

I

I

G

G

G
|
G

S
x
N

L

I

A
x
I

H
x
T

A

A

D

S

P
|
P

A

I

A

S

D
|
D

W

L

V

N

N

P

V

V

L

F

T

M

A

A

L

S

G

G

A

A

D

K

V

L

V

T

I

L

V

V

G

S

Q

R

G

G

G

T

R

R

S

T

V

V

P

R

M

M

T

D

-

H

D

T

F

F

I

F

L

P

G

G

V
x
Y

F
x
R

E

K

T

T

A

L

L

L

Q

R

P

P

G

E

E

E

I
|
I

V
x
L

A

L

E

S

I

I

H

E

V

I

P

P

A

Y

L

S

S

K

E

E

R

G

A

E

R

F

W

F

G

S

Y

A

Y

F

K

K

-

Q

V

A

C

S

R

R

K

R

T

E

G

A

E

D

F

I

S

A

H

K

A

V

T

T

G

S

A

G

-

M

-

R

V

V

L

L

I

F

D

K

P

P

A

G

binding flavin-adenine dinucleotide: L227 (≠ P30), V228 (= V31), V229 (≠ A32), G230 (= G33), N231 (≠ S34), T232 (≠ Q35), E233 (≠ S36), I234 (≠ L37), F307 (≠ I101), V312 (= V106), S317 (≠ T111), G320 (= G114), N321 (≠ S115), I323 (≠ A117), T324 (≠ H118), D330 (= D124), I373 (= I166), L374 (≠ V167)
binding nicotinamide-adenine-dinucleotide: P327 (= P121), Y363 (≠ V156), R364 (≠ F157)

Sites not aligning to the query:

active site: 738, 773, 851, 855, 883, 1231, 1232
binding bicarbonate ion: 810, 811, 880, 881, 885, 886, 889
binding flavin-adenine dinucleotide: 44
binding fe2/s2 (inorganic) cluster: 40, 41, 42, 44, 46, 47, 49, 69, 71, 109, 110, 111, 113, 145, 147, 715
binding nicotinamide-adenine-dinucleotide: 428, 430, 431, 471, 478
binding uric acid: 773, 851, 885, 980, 981, 1049, 1050, 1232

P22985 Xanthine dehydrogenase/oxidase; EC 1.17.1.4; EC 1.17.3.2 from Rattus norvegicus (Rat) (see 2 papers)
25% identity, 73% coverage: 8:205/270 of query aligns to 235:444/1331 of P22985

query
sites
P22985

Y

W

A

I

Q

Q

P

A

A

S

T

T

L

M

D

E

A

E

A

L

L

L

E

D

L

L

L

K

S

A

R

Q

E

H

D

P

V

-

M

D

V

A

R

K

P
x
L

V
|
V

A
x
V

G
|
G

S
x
N

Q
x
T

S
x
E

L
x
I

G

G

P

I

M

E

L

M

N

K

L

F

R

K

L

N

A

M

Q

L

P

F

E

P

L

L

L

I

V

V

D

C

I

P

T

A

R

W

I

I

A

P

E

E

L

L

R

N

T

S

I

V

R

V

R

H

E

G

G

P

D

E

R

G

L

I

V

S

I

F

G

G

A

A

C

S

V

C

T

P

H

L

A

S

R

L

L

V

E

E

D

S

-

V

-

L

-

A

-

E

-

I

G

A

D

K

Y

L

P

P

D

E

-

Q

-

K

-

T

-

E

V

V

T

F

R

R

G

G

V

V

L

M

P

E

S

Q

V

L

A

R

A
x
W

V

-

I
x
F

A

A

Y

G

R

K

A

Q

V

V

R

K

N

S

R

V

G

A

T
x
S

I
|
I

G
|
G

S
x
N

L

I

A

I

H

T

A

A

D

S

P

P

A

I

A

S

D
|
D

W

L

V

N

N

P

V

V

L

F

T

M

A

A

L

S

G

G

A

A

D

K

V

L

V

T

I

L

V

V

G

S

Q

R

G

G

G

T

R

R

S

T

V

V

P

R

M

M

T

D

-

H

D

T

F

F

I

F

L

P

G

G

V

Y

F

R

E

K

T

T

A

L

L

L

Q

R

P

P

G

E

E

E

I

I

V
x
L

A

L

E

S

I

I

H

E

V

I

P

P

A

Y

L

S

S

K

E

E

R

G

A

E

R

F

W

F

G

S

Y

A

Y

F

K

K

-

Q

V

A

C

S

R

R

K

R

T

E

G

D

E

D

F

I

S

A

H

K

A

V

T

T

G

S

A

G

-

M

-

R

V

V

L

L

I

F

D

K

P

P

A

G

256:263 (vs. 30:37, 25% identical) binding FAD
W-F 335:336 (≠ AVI 99:101) mutation to AL: Converts the enzyme to the oxidase form that utilizes molecular oxygen as electron acceptor. Interferes with normal conversion to the dehydrogenase form by reducing agents.
SIGGN 346:350 (≠ TIGGS 111:115) binding FAD
D359 (= D124) binding FAD
L403 (≠ V167) binding FAD

Sites not aligning to the query:

43 binding [2Fe-2S] cluster
48 binding [2Fe-2S] cluster
51 binding [2Fe-2S] cluster
73 binding [2Fe-2S] cluster
112 binding [2Fe-2S] cluster
115 binding [2Fe-2S] cluster
147 binding [2Fe-2S] cluster
149 binding [2Fe-2S] cluster
535 C→A: Slows the conversion from the dehydrogenase form to the oxidase form; when associated with R-992. Abolishes conversion from the dehydrogenase form to the oxidase form; when associated with R-992 and S-1316.
992 C→R: Slows the conversion from the dehydrogenase form to the oxidase form; when associated with A-535. Abolishes conversion from the dehydrogenase form to the oxidase form; when associated with A-535 and S-1316.
1316 C→S: Abolishes conversion from the dehydrogenase form to the oxidase form; when associated with A-535 and R-992.

P80457 Xanthine dehydrogenase/oxidase; EC 1.17.1.4; EC 1.17.3.2 from Bos taurus (Bovine) (see 5 papers)
25% identity, 66% coverage: 8:185/270 of query aligns to 236:422/1332 of P80457

query
sites
P80457

Y

W

A

I

Q

Q

P

A

A

S

T

T

L

L

D

K

A

E

A

L

L

L

E

D

L

L

L

K

S

A

R

Q

E

H

D

P

V

-

M

E

V

A

R

K

P
x
L

V
|
V

A
x
V

G
|
G

S
x
N

Q
x
T

S
x
E

L
x
I

G

G

P

I

M

E

L

M

N

K

L

F

R

K

L

N

A

Q

Q

L

P

F

E

P

L

M

L

I

V

I

D

C

I

P

T

A

R

W

I

I

A

P

E

E

L

L

R

N

T

A

I

V

R

E

R

H

E

G

G

P

D

E

R

G

L

I

V

S

I

F

G

G

A

A

C

A

V

C

T

A

H

L

A

S

R

S

L

V

E

E

D

K

-

T

-

L

-

E

-

A

-

V

G

A

D

K

Y

L

P

P

-

T

-

Q

-

K

-

T

D

E

V

V

T

F

R

R

G

G

V

V

L

L

P

E

S

Q

V

L

A
x
R

A
x
W

V

-

I
x
F

A

A

Y

G

R

K

A

Q

V

V

R

K

N

S

R

V

G

A

T
x
S

I
x
L

G
|
G

S
x
N

L

I

A

I

H

T

A

A

D

S

P

P

A

I

A

S

D
|
D

W

L

V

N

N

P

V

V

L

F

T

M

A

A

L

S

G

G

A

T

D

K

V

L

V

T

I

I

V

V

G

S

Q

R

G

G

G

T

R

R

S

T

V

V

P

P

M

M

T

D

-

H

D

T

F

F

I

F

L

P

G

S

V

Y

F

R

E

K

T

T

A

L

L

L

Q

G

P

P

G

E

E

E

I

I

V
x
L

A

L

E

S

I

I

H

E

V

I

P

P

A

Y

L

S

S

R

E

E

R

D

A

E

R

F

W

F

G

S

Y

A

Y

F

K
|
K

257:264 (vs. 30:37, 25% identical) binding FAD
R335 (≠ A98) mutation to A: Promotes conversion to the oxidase form that utilizes molecular oxygen as electron acceptor. Interferes with normal conversion to the dehydrogenase form by reducing agents.
W336 (≠ A99) mutation to A: Promotes conversion to the oxidase form that utilizes molecular oxygen as electron acceptor. Interferes with normal conversion to the dehydrogenase form by reducing agents.
F337 (≠ I101) binding FAD
SLGGN 347:351 (≠ TIGGS 111:115) binding FAD
D360 (= D124) binding FAD
L404 (≠ V167) binding FAD
K422 (= K185) binding FAD

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
43 binding [2Fe-2S] cluster
48 binding [2Fe-2S] cluster
51 binding [2Fe-2S] cluster
73 binding [2Fe-2S] cluster
113 binding [2Fe-2S] cluster
116 binding [2Fe-2S] cluster
148 binding [2Fe-2S] cluster
150 binding [2Fe-2S] cluster
427 R→Q: Promotes conversion to the oxidase form that utilizes molecular oxygen as electron acceptor. Interferes with normal conversion to the dehydrogenase form by reducing agents.
767 binding Mo-molybdopterin
798 binding Mo-molybdopterin
912 binding Mo-molybdopterin
1079 binding Mo-molybdopterin
1261 active site, Proton acceptor

1n5xA Xanthine dehydrogenase from bovine milk with inhibitor tei-6720 bound (see paper)
25% identity, 66% coverage: 8:185/270 of query aligns to 208:394/1290 of 1n5xA

query
sites
1n5xA

Y

W

A

I

Q

Q

P

A

A

S

T

T

L

L

D

K

A

E

A

L

L

L

E

D

L

L

L

K

S

A

R

Q

E

H

D

P

V

-

M

E

V

A

R

K

P
x
L

V

V

A
x
V

G
|
G

S
x
N

Q
x
T

S
x
E

L
x
I

G

G

P

I

M

E

L

M

N

K

L

F

R

K

L

N

A

Q

Q

L

P

F

E

P

L

M

L

I

V

I

D

C

I

P

T

A

R

W

I

I

A

P

E

E

L

L

R

N

T

A

I

V

R

E

R

H

E

G

G

P

D

E

R

G

L

I

V

S

I

F

G

G

A

A

C

A

V

C

T

A

H

L

A

S

R

S

L

V

E

E

D

K

-

T

-

L

-

E

-

A

-

V

G

A

D

K

Y

L

P

P

-

T

-

Q

-

K

-

T

D

E

V

V

T

F

R

R

G

G

V

V

L

L

P

E

S

Q

V

L

A

R

A

W

V

-

I
x
F

A
|
A

Y

G

R

K

A

Q

V

V

R

K

N

S

R

V

G
x
A

T
x
S

I

L

G

G

G
|
G

S
x
N

L

I

A
x
I

H
x
T

A

A

D

S

P

P

A

I

A

S

D
|
D

W

L

V

N

N

P

V

V

L

F

T

M

A

A

L

S

G

G

A

T

D

K

V

L

V

T

I

I

V

V

G

S

Q

R

G

G

G

T

R

R

S

T

V

V

P

P

M

M

T

D

-

H

D

T

F

F

I

F

L

P

G

S

V

Y

F

R

E

K

T

T

A

L

L

L

Q

G

P

P

G

E

E

E

I

I

V
x
L

A

L

E

S

I

I

H

E

V

I

P

P

A

Y

L

S

S

R

E

E

R

D

A

E

R

F

W

F

G

S

Y

A

Y

F

K

K

binding flavin-adenine dinucleotide: L229 (≠ P30), V231 (≠ A32), G232 (= G33), N233 (≠ S34), T234 (≠ Q35), E235 (≠ S36), I236 (≠ L37), F309 (≠ I101), A310 (= A102), A318 (≠ G110), S319 (≠ T111), G322 (= G114), N323 (≠ S115), I325 (≠ A117), T326 (≠ H118), D332 (= D124), L376 (≠ V167)

Sites not aligning to the query:

active site: 734, 769, 847, 851, 879, 1227, 1228
binding flavin-adenine dinucleotide: 43, 44
binding fe2/s2 (inorganic) cluster: 40, 41, 42, 44, 46, 47, 49, 71, 110, 111, 112, 114, 146, 148, 711
binding 2-(3-cyano-4-isobutoxy-phenyl)-4-methyl-5-thiazole-carboxylic acid: 615, 738, 769, 840, 847, 881, 976, 977, 978, 981

Query Sequence

>AZOBR_RS29695 FitnessBrowser__azobra:AZOBR_RS29695
MKAVDFDYAQPATLDAALELLSREDVMVRPVAGSQSLGPMLNLRLAQPELLVDITRIAEL
RTIRREGDRLVIGACVTHARLEDGDYPDVTRGVLPSVAAVIAYRAVRNRGTIGGSLAHAD
PAADWVNVLTALGADVVIVGQGGRRSVPMTDFILGVFETALQPGEIVAEIHVPALSERAR
WGYYKVCRKTGEFSHATGAVLIDPARGVQRCVAGATSGKPVVIDGPALFTDGCTEAALAA
HLAGSPAADDPIALRTHTVALKRAIAQVMS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory