SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AZOBR_RS30390 FitnessBrowser__azobra:AZOBR_RS30390 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q53IZ1 Bifunctional aspartate aminotransferase and L-aspartate beta-decarboxylase; Aspartate 4-decarboxylase; Asd; AsdP; EC 2.6.1.1; EC 4.1.1.12 from Pseudomonas sp. (see paper)
73% identity, 97% coverage: 3:526/543 of query aligns to 2:525/531 of Q53IZ1

query
sites
Q53IZ1

S

S

I

K

D

D

Y

Y

S

R

R

S

Y

L

A

A

K

N

L

L

S

S

P

P

F

F

E

E

L

L

K

K

D

D

E

E

L

L

I

I

K

K

L

V

A

A

S

S

G

G

R

K

E

A

N

N

R

R

L

L

M

M

L

L

N

N

A

A

G

G

R

R

G

G

N

N

P

P

N

N

F

F

L

L

A

A

T

T

L

T

P

P

R

R

A

A

F

F

R

R

L

L

G

G

L

L

F

F

A

A

V

A

A

A

E

E

A

S

E

E

L

L

S

S

F

Y

S
|
S

Y
|
Y

M
|
M

P

T

N

V

G

G

V

V

G

G

L

L

P

A

R

K

L

L

E

D

G

G

I

I

E

E

G

G

R

R

F

F

E

E

R

R

Y

F

V

I

S

A

E

E

H

H

R

R

D

D

Q

Q

E

E

G

G

V

V

V

K

F

F

L

L

G

G

R

K

A

S

L

L

S

S

Y

Y

V

V

R

R

D

D

Q

Q

L

L

G

G

L

L

S

D

G

P

S

A

G

A

F

F

L

L

H

H

E

E

M

M

V

V

E

D

G

G

V

I

L

L

G

G

A

C

N

N

Y

Y

P

P

V

V

P

P

R

R

M

M

L

L

A

T

I

V

S

S

E

E

D

Q

V

I

V

V

R

R

H

Q

Y

Y

L

I

V

V

K

R

E

E

M

M

V

A

G

G

F

A

L

V

P

P

G

E

S

S

V

V

D

D

L

L

F

F

A

A

V

V

E

E

G

G

T

T

A

A

M

M

T

A

Y

Y

I

I

F

F

N

E

S

S

M

L

K

R

Q

I

N

S

G

G

L

L

V

L

E

K

R

A

G

G

D

D

K

K

V

V

A

A

I

I

G

G

L

M

P

P

V

V

F

F

T

T

P

P

Y

Y

I

I

E

E

I

I

P

P

E

E

L

L

D

A

E

Q

Y

Y

G

D

L

L

T

K

E

E

V

V

A

P

I

I

N

H

A

A

D

D

P

P

A

D

K

N

G

G

W

W

Q

Q

Y

Y

P

S

D

D

A

A

E

E

L

L

D

D

K

K

L

L

K

K

D

D

P

P

A

D

V

V

K

K

V

I

F

F

C

C

V

V

N

N

P

P

S

S

N

N

P

P

S

S

V

V

K

K

M

M

D

D

D

Q

R

R

S

S

L

L

D

D

R

R

I

V

A

R

A

A

I

I

V

V

K

A

N

E

E

Q

R

R

K

P

D

D

L

L

I

L

I

I

L

L

T

T

D

D

V

V

Y

Y

G

G

T

T

F

F

A

A

D

D

N

E

F

F

R

Q

S

S

L

L

F

F

A

S

V

V

C

C

P

P

E

R

N

N

T

T

M

L

L

L

V

V

Y

Y

S

S

F

F

S

S

K
|
K

Y

Y

F

F

G

G

A

A

T

T

G

G

W

W

R

R

L

L

G

G

V

V

I

I

A

A

T

A

H

H

K

K

T

D

N

N

V

V

C

F

D

D

Q

H

R

A

I

L

A

S

A

Q

L

L

P

P

E

E

E

S

R

A

K

K

A

K

A

A

L

L

D

D

R

H

R

R

Y

Y

G

R

S

S

L

L

V

L

P

P

D

D

V

V

R

R

G

S

L

L

R

K

F

F

I

I

D

D

R

R

L

L

V

V

A

A

D

D

S

S

R

R

T

V

V

V

A

A

L

L

N

N

H

H

T

T

A

A

G

G

L

L

S

S

T

T

P

P

Q

Q

V

V

Q

Q

M

M

V

V

L

L

F

F

S

S

L

L

F

F

A

A

L

L

M

M

D

D

E

E

Q

A

D

D

G

A

Y

Y

K

K

A

Q

E

A

L

L

K

K

K

Q

V

L

I

I

R

R

E

E

A

A

A

T

L

L

Y

Y

R

R

E

E

L

L

G

G

L

M

P

P

T

P

L

L

S

E

D

N

P

P

N

N

A

S

V

V

D

N

Y

Y

T

T

L

L

L

I

D

D

L

L

E

Q

D

N

I

V

A

T

L

C

K

R

L

L

Y

Y

G

G

P

E

D

A

Y

F

A

S

A

Q

W

W

V

A

K

V

A

Q

N

Q

F

S

V

S

P

T

N

G

D

D

L

M

L

L

F

F

R

R

I

V

A

A

A

D

E

E

T

T

G

G

I

I

V

V

L

L

P

P

G

G

K

R

G

G

F

F

G

G

T

S

L

D

Q

R

P

P

A

S

A

G

R

R

V

A

S

S

L

L

A

A

N

N

L

L

N

N

E

E

Y

Y

E

E

Y

Y

A

A

I

I

G

G

R

R

S

A

L

L

R

R

G

R

M

L

A

A

D

D

Q

E

S

L

H

Y

E

E

E

Q

F

Y

SYM 67:69 (= SYM 68:70) mutation to EEE: Loss of activity. Dissociation into dimers.
K314 (= K315) modified: N6-(pyridoxal phosphate)lysine

2zy2A Dodecameric l-aspartate beta-decarboxylase (see paper)
73% identity, 95% coverage: 13:526/543 of query aligns to 1:514/521 of 2zy2A

query
sites
2zy2A

S

S

P

P

F

F

E

E

L

L

K

K

D

D

E

E

L

L

I

I

K

K

L

V

A

A

S

S

G

G

R

K

E

A

N

N

R

R

L

L

M

M

L

L

N

N

A

A

G

G

R
|
R

G

G

N

N

P

P

N

N

F

F

L

L

A

A

T

T

L

T

P

P

R

R

A

A

F

F

R

R

L

L

G

G

L

L

F

F

A

A

V

A

A

A

E

E

A

S

E

E

L

L

S

S

F

Y

S

S

Y

Y

M

M

P

T

N

V

G

G

V

V

G

G

L

L

P

A

R

K

L

L

E

D

G

G

I

I

E

E

G

G

R

R

F

F

E

E

R

R

Y

F

V

I

S

A

E

E

H

H

R

R

D

D

Q

Q

E

E

G

G

V

V

V

K

F

F

L

L

G

G

R

K

A

S

L

L

S

S

Y

Y

V

V

R

R

D

D

Q

Q

L

L

G

G

L

L

S

D

G

P

S

A

G

A

F

F

L

L

H

H

E

E

M

M

V

V

E

D

G

G

V

I

L

L

G

G

A

C

N

N

Y

Y

P

P

V

V

P

P

R

R

M

M

L

L

A

T

I

V

S

S

E

E

D

Q

V

I

V

V

R

R

H

Q

Y

Y

L

I

V

V

K

R

E

E

M

M

V

A

G

G

F

A

L

V

P

P

G

E

S

S

V

V

D

D

L

L

F

F

A

A

V

V

E

E

G
|
G

T
|
T

A

A

M

M

T

A

Y

Y

I

I

F

F

N

E

S

S

M

L

K

R

Q

I

N

S

G

G

L

L

V

L

E

K

R

A

G

G

D

D

K

K

V

V

A

A

I

I

G

G

L

M

P

P

V

V

F
|
F

T

T

P

P

Y

Y

I

I

E

E

I

I

P

P

E

E

L

L

D

A

E

Q

Y

Y

G

D

L

L

T

K

E

E

V

V

A

P

I

I

N

H

A

A

D

D

P

P

A

D

K

N

G

G

W

W

Q

Q

Y

Y

P

S

D

D

A

A

E

E

L

L

D

D

K

K

L

L

K

K

D

D

P

P

A

D

V

V

K

K

V

I

F

F

C

C

V

V

N

N

P

P

S

S

N

N

P

P

S

S

V

V

K

K

M

M

D

D

D

Q

R

R

S

S

L

L

D

D

R

R

I

V

A

R

A

A

I

I

V

V

K

A

N

E

E

Q

R

R

K

P

D

D

L

L

I

L

I

I

L

L

T

T

D
|
D

D

D

V

V

Y
|
Y

G

G

T

T

F

F

A

A

D

D

N

E

F

F

R

Q

S

S

L

L

F

F

A

S

V

V

C

C

P

P

E

R

N

N

T

T

M

L

L

L

V

V

Y

Y

S
|
S

F

F

S

S

K
|
K

Y

Y

F

F

G

G

A

A

T

T

G

G

W

W

R
|
R

L

L

G

G

V

V

I

I

A

A

T

A

H

H

K

K

T

D

N

N

V

V

C

F

D

D

Q

H

R

A

I

L

A

S

A

Q

L

L

P

P

E

E

E

S

R

A

K

K

A

K

A

A

L

L

D

D

R

H

R

R

Y

Y

G

R

S

S

L

L

V

L

P

P

D

D

V

V

R

R

G

S

L

L

R

K

F

F

I

I

D

D

R

R

L

L

V

V

A

A

D

D

S

S

R

R

T

V

V

V

A

A

L

L

N

N

H

H

T

T

A

A

G

G

L

L

S

S

T

T

P

P

Q

Q

V

V

Q

Q

M

M

V

V

L

L

F

F

S

S

L

L

F

F

A

A

L

L

M

M

D

D

E

E

Q

A

D

D

G

A

Y

Y

K

K

A

Q

E

A

L

L

K

K

K

Q

V

L

I

I

R

R

E

E

A

A

A

T

L

L

Y

Y

R

R

E

E

L

L

G

G

L

M

P

P

T

P

L

L

S

E

D

N

P

P

N

N

A

S

V

V

D

N

Y

Y

T

T

L

L

L

I

D

D

L

L

E

Q

D

N

I

V

A

T

L

C

K

R

L

L

Y

Y

G

G

P

E

D

A

Y

F

A

S

A

Q

W

W

V

A

K

V

A

Q

N

Q

F

S

V

S

P

T

N

G

D

D

L

M

L

L

F

F

R

R

I

V

A

A

A

D

E

E

T

T

G

G

I

I

V

V

L

L

P

P

G

G

K

R

G

G

F

F

G

G

T

S

L

D

Q

R

P

P

A

S

A

G

R

R

V

A

S

S

L

L

A

A

N

N

L

L

N

N

E

E

Y

Y

E

E

Y

Y

A

A

I

I

G

G

R

R

S

A

L

L

R

R

G

R

M

L

A

A

D

D

Q

E

S

L

H

Y

E

E

E

Q

F

Y

binding pyridoxal-5'-phosphate: R26 (= R38), G161 (= G173), G162 (= G174), T163 (= T175), F192 (= F204), D274 (= D286), Y277 (= Y289), S300 (= S312), K303 (= K315), R311 (= R323)

Q93QX0 Bifunctional aspartate aminotransferase and L-aspartate beta-decarboxylase; Aspartate 4-decarboxylase; ASD; AsdA; EC 2.6.1.1; EC 4.1.1.12 from Comamonas testosteroni (Pseudomonas testosteroni) (see paper)
72% identity, 97% coverage: 3:531/543 of query aligns to 2:531/533 of Q93QX0

query
sites
Q93QX0

S

S

I

K

D

D

Y

Y

S

Q

R

S

Y

L

A

A

K

K

L

L

S

S

P

P

F

F

E

E

L

L

K

K

D

D

E

E

L

L

I

I

K

K

L

I

A

A

S

S

G

S

R

D

E

G

N

N

R

R

L

L

M

M

L

L

N

N

A

A

G

G

R

R

G

G

N

N

P

P

N

N

F

F

L

L

A

A

T

T

L

T

P

P

R

R

A

A

F

F

R

R

L

L

G

G

L

L

F

F

A

A

V

A

A

A

E

E

A

S

E

E

L

L

S

S

F

Y

S

S

Y

Y

M

M

P

T

N

T

-

V

G

G

V

V

G

G

L

L

P

A

R

K

L

I

E

D

G

G

I

I

E

E

G

G

R

R

F

F

E

E

R

R

Y

Y

V

I

S

A

E

E

H

N

R

R

D

D

Q

Q

E

E

G

G

V

V

V

R

F

F

L

L

G

G

R

K

A

S

L

L

S

S

Y

Y

V

V

R

R

D

D

Q

Q

L

L

G

G

L

L

S

D

G

P

S

A

G

A

F

F

L

L

H

H

E

E

M

M

V

V

E

D

G

G

V

I

L

L

G

G

A

C

N

N

Y
|
Y

P

P

V

V

P

P

R

R

M

M

L

L

A

N

I

I

S

S

E

E

D

K

V

I

V

V

R

R

H

Q

Y

Y

L

I

V

I

K

R

E

E

M

M

V

G

G

A

G

D

F

A

L

I

P

P

P

S

G

E

S

S

V

V

D

N

L

L

F

F

A

A

V

V

E

E

G

G

T

T

A

A

M

M

T

A

Y

Y

I

I

F

F

N

E

S

S

M

L

K

K

Q

L

N

N

G

G

L

L

V

L

E

K

R

A

G

G

D

D

K

K

V

V

A

A

I

I

G

G

L

M

P

P

V

V

F

F

T

T

P

P

Y

Y

I

I

E

E

I

I

P

P

E

E

L

L

D

A

E

Q

Y

Y

G

A

L

L

T

E

E

E

V

V

A

A

I

I

N

N

A

A

D

D

P

P

A

S

K

L

G

N

W

W

Q

Q

Y

Y

P

P

D

D

A

S

E

E

L

L

D

D

K

K

L

L

K

K

D

D

P

P

A

A

V

I

K

K

V

I

F

F

C

C

V

V

N

N

P

P

S

S

N

N

P

P

S

S

V

V

K

K

M

M

D

D

D

Q

R

R

S

S

L

L

D

E

R

R

I

V

A

R

A

N

I

I

V

V

K

A

N

E

E

H

R

R

K

P

D

D

L

L

I

M

I

I

L

L

T

T

D

D

V

V

Y

Y

G

G

T

T

F

F

A

A

D

D

N

D

F

F

R

Q

S

S

L

L

F

F

A

A

V

I

C

C

P

P

E

E

N

N

T

T

M

L

L

L

V

V

Y

Y

S

S

F

F

S

S

K
|
K

Y

Y

F

F

G

G

A

A

T

T

G

G

W

W

R

R

L

L

G

G

V

V

I

V

A

A

T

A

H

H

K

Q

T

Q

N

N

V

V

C

F

D

D

Q

L

R

A

I

L

A

D

A

K

L

L

P

Q

E

E

E

S

R

E

K

K

A

V

A

A

L

L

D

D

R

H

R

R

Y

Y

G

R

S

S

L

L

V

L

P

P

D

D

V

V

R

R

G

S

L

L

R

K

F

F

I

I

D

D

R

R

L

L

V

V

A

A

D

D

S

S

R

R

T

A

V

V

A

A

L

L

N

N

H

H

T

T

A

A

G

G

L

L

S

S

T

T

P

P

Q

Q

V

V

Q

Q

M

M

V

A

L

L

F

F

S

S

L

L

F

F

A

A

L

L

M

M

D

D

E

E

Q

A

D

D

G

E

Y

Y

K

K

A

H

E

T

L

L

K

K

K

Q

V

L

I

I

R

R

E

E

A

T

L

L

Y

Y

R

R

E

E

L

L

G

G

L

M

P

P

T

P

L

L

S

R

D

D

P

E

N

N

A

A

V

V

D

D

Y

Y

T

T

L

L

L

I

D

D

L

L

E

Q

D

D

I

V

A

T

L

A

K

K

L

L

Y

Y

G

G

P

E

D

A

Y

F

A

S

A

E

W

W

V

A

K

V

A

K

N

Q

F

S

V

S

P

T

N

G

D

D

L

M

L

L

F

F

R

R

I

I

A

A

A

D

E

E

T

T

G

G

I

I

V

V

L

L

P

P

G

G

K
x
R

G

G

F

F

G

G

T

S

L

N

Q

R

P

P

A

S

A

G

R

R

V

A

S

S

L

L

A

A

N

N

L

L

N

N

E

E

Y

Y

E

E

Y

Y

A

A

I

I

G

G

R

R

S

A

L

L

R

R

G

K

M

M

A

A

D

D

Q

E

S

L

H

Y

E

A

E

E

F

Y

T

S

R

G

Q

Q

R

A

Q

Q

Y134 (= Y134) mutation to F: Slightly reduced activity.
K315 (= K315) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Slightly reduced activity.
R487 (≠ K487) mutation to A: Loss of activity.

2zy4F Dodecameric l-aspartate beta-decarboxylase (see paper)
72% identity, 96% coverage: 10:531/543 of query aligns to 4:526/535 of 2zy4F

query
sites
2zy4F

A

A

K

K

L

L

S

S

P

P

F

F

E

E

L

L

K

K

D

D

E

E

L

L

I

I

K

K

L

I

A

A

S

S

G

S

R

D

E

G

N

N

R

R

L

L

M

M

L

L

N

N

A

A

G

G

R
|
R

G

G

N

N

P

P

N

N

F

F

L

L

A

A

T

T

L

T

P

P

R

R

A

A

F

F

R

R

L

L

G

G

L

L

F

F

A

A

V

A

A

A

E

E

A

S

E

E

L

L

S

S

F

Y

S

S

Y

Y

M

M

P

T

N

T

-

V

G

G

V

V

G

G

L

L

P

A

R

K

L

I

E

D

G

G

I

I

E

E

G

G

R

R

F

F

E

E

R

R

Y

Y

V

I

S

A

E

E

H

N

R

R

D

D

Q

Q

E

E

G

G

V

V

V

R

F

F

L

L

G

G

R

K

A

S

L

L

S

S

Y

Y

V

V

R

R

D

D

Q

Q

L

L

G

G

L

L

S

D

G

P

S

A

G

A

F

F

L

L

H

H

E

E

M

M

V

V

E

D

G

G

V

I

L

L

G

G

A

C

N

N

Y

Y

P

P

V

V

P

P

R

R

M

M

L

L

A

N

I

I

S

S

E

E

D

K

V

I

V

V

R

R

H

Q

Y

Y

L

I

V

I

K

R

E

E

M

M

V

G

G

A

G

D

F

A

L

I

P

P

P

S

G

E

S

S

V

V

D

N

L

L

F

F

A

A

V

V

E

E

G
|
G

T
|
T

A

A

M

M

T

A

Y

Y

I

I

F

F

N

E

S

S

M

L

K

K

Q

L

N

N

G

G

L

L

V

L

E

K

R

A

G

G

D

D

K

K

V

V

A

A

I

I

G

G

L

M

P

P

V

V

F
|
F

T

T

P

P

Y

Y

I

I

E

E

I

I

P

P

E

E

L

L

D

A

E

Q

Y

Y

G

A

L

L

T

E

E

E

V

V

A

A

I

I

N

N

A

A

D

D

P

P

A

S

K

L

G

N

W

W

Q

Q

Y

Y

P

P

D

D

A

S

E

E

L

L

D

D

K

K

L

L

K

K

D

D

P

P

A

A

V

I

K

K

V

I

F

F

C

C

V

V

N

N

P

P

S

S

N

N

P

P

S

S

V

V

K

K

M

M

D

D

D

Q

R

R

S

S

L

L

D

E

R

R

I

V

A

R

A

N

I

I

V

V

K

A

N

E

E

H

R

R

K

P

D

D

L

L

I

M

I

I

L

L

T

T

D
|
D

D

D

V
|
V

Y
|
Y

G

G

T

T

F

F

A

A

D

D

N

D

F

F

R

Q

S

S

L

L

F

F

A

A

V

I

C

C

P

P

E

E

N

N

T

T

M

L

L

L

V

V

Y

Y

S
|
S

F

F

S
|
S

K
|
K

Y

Y

F

F

G

G

A

A

T

T

G

G

W

W

R
|
R

L

L

G

G

V

V

I

V

A

A

T

A

H

H

K

Q

T

Q

N

N

V

V

C

F

D

D

Q

L

R

A

I

L

A

D

A

K

L

L

P

Q

E

E

E

S

R

E

K

K

A

V

A

A

L

L

D

D

R

H

R

R

Y

Y

G

R

S

S

L

L

V

L

P

P

D

D

V

V

R

R

G

S

L

L

R

K

F

F

I

I

D

D

R

R

L

L

V

V

A

A

D

D

S

S

R

R

T

A

V

V

A

A

L

L

N

N

H

H

T

T

A

A

G

G

L

L

S

S

T

T

P

P

Q

Q

V

V

Q

Q

M

M

V

A

L

L

F

F

S

S

L

L

F

F

A

A

L

L

M

M

D

D

E

E

Q

A

D

D

G

E

Y

Y

K

K

A

H

E

T

L

L

K

K

K

Q

V

L

I

I

R

R

E

E

A

T

L

L

Y

Y

R

R

E

E

L

L

G

G

L

M

P

P

T

P

L

L

S

R

D

D

P

E

N

N

A

A

V

V

D

D

Y

Y

T

T

L

L

L

I

D

D

L

L

E

Q

D

D

I

V

A

T

L

A

K

K

L

L

Y

Y

G

G

P

E

D

A

Y

F

A

S

A

E

W

W

V

A

K

V

A

K

N

Q

F

S

V

S

P

T

N

G

D

D

L

M

L

L

F

F

R

R

I

I

A

A

A

D

E

E

T

T

G

G

I

I

V

V

L

L

P

P

G

G

K

R

G

G

F

F

G

G

T

S

L

N

Q

R

P

P

A

S

A

G

R

R

V

A

S

S

L

L

A

A

N

N

L

L

N

N

E

E

Y

Y

E

E

Y

Y

A

A

I

I

G

G

R

R

S

A

L

L

R

R

G

K

M

M

A

A

D

D

Q

E

S

L

H

Y

E

A

E

E

F

Y

T

S

R

G

Q

Q

R

A

Q

Q

binding pyridoxal-5'-phosphate: R32 (= R38), G168 (= G173), G169 (= G174), T170 (= T175), F199 (= F204), D281 (= D286), V283 (= V288), Y284 (= Y289), S307 (= S312), S309 (= S314), K310 (= K315), R318 (= R323)

8wkjA The crystal structure of aspartate aminotransferases lpg0070 from legionella pneumophila (see paper)
27% identity, 25% coverage: 194:328/543 of query aligns to 115:251/391 of 8wkjA

query
sites
8wkjA

G

G

D

D

K

E

V

V

A

I

I

I

G

P

L

A

P

P

V

Y

F
x
W

T

V

P

S

Y

Y

I

P

E

D

I

M

P

V

E

L

L

L

D

A

E

D

Y

G

G

-

L

-

T

V

E

P

V

V

A

I

I

I

N

E

A

T

D

T

P

P

A

A

K

Q

G

R

W

Y

Q

K

Y

I

P

N

D

A

A

Q

E

Q

L

L

D

E

K

Q

L

A

K

I

D

T

P

P

A

K

V

T

K

R

V

M

F

I

F

F

C

L

V
x
N

N

S

P

P

S

S

N
|
N

P

P

P

S

S

G

V

I

K

A

M

Y

D

T

D

Q

R

N

S

E

L

L

D

K

R

E

I

L

A

G

A

D

I

V

V

L

K

K

N

-

E

K

R

H

K

P

D

Q

L

I

I

L

I

I

L

A

T

T

D
|
D

D

D

V

M

Y
|
Y

G

E

-

H

-

I

T

I

F

W

A

S

D

Q

N

P

F

F

R

T

S

N

L

I

F

L

A

N

V

A

C

C

P

P

E

E

-

L

-

Y

-

D

N

R

T

T

M

I

L

V

V

L

Y

N

S

G

F

V

S
|
S

K
|
K

Y

A

F

Y

G

A

A

M

T

T

G

G

W

W

R
|
R

L

I

G

G

V

Y

I

A

A

A

binding pyridoxal-5'-phosphate: W125 (≠ F204), N171 (≠ V252), N175 (= N256), D204 (= D286), Y207 (= Y289), S237 (= S314), K238 (= K315), R246 (= R323)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 99, 100, 101

1gdeA Crystal structure of pyrococcus protein a-1 e-form (see paper)
28% identity, 27% coverage: 185:328/543 of query aligns to 101:245/388 of 1gdeA

query
sites
1gdeA

M

M

K

G

Q

L

N

S

G

A

L

F

V

L

E

K

R

D

G

G

D

E

K

E

V

V

A

L

I

I

G

P

L

T

P

P

V

A

F
|
F

T

V

P

S

Y

Y

-

A

-

P

-

A

-

V

-

I

-

L

-

A

-

G

-

K

-

P

I

V

E

E

I

V

P

P

E

T

L

Y

D

E

E

E

Y

-

G

-

L

-

T

D

E

E

V

F

A

R

I

L

N

N

A

V

D

D

P

-

A

-

K

-

G

-

W

-

Q

-

Y

-

P

-

D

-

A

-

E

E

L

L

D

K

K

K

L

Y

K

V

D

T

P

D

A

K

V

T

K

R

V

A

F

L

F

I

C

I

V
x
N

N

S

P

P

S

C

N
|
N

P

P

P

T

S

G

V

A

K

V

M

L

D

T

D

K

R

K

S

D

L

L

D

E

R

E

I

I

A

A

A

D

I

F

V

V

K

V

N

E

E

H

R

-

K

-

D

D

L

L

I

I

I

V

L

I

T

S

D
|
D

D

E

V

V

Y
|
Y

G

E

T

H

F

F

A

I

D

Y

N

D

F

D

R

A

S

R

L

H

F

Y

A

S

V

I

C

A

P

S

-

L

-

D

-

G

-

M

-

F

E

E

N

R

T

T

M

I

L

T

V

V

Y

N

S

G

F

F

S
|
S

K
|
K

Y

T

F

F

G

A

A

M

T

T

G

G

W

W

R
|
R

L

L

G

G

V

F

I

V

A

A

active site: K232 (= K315)
binding glutamic acid: F120 (= F204), N170 (= N256)
binding pyridoxal-5'-phosphate: F120 (= F204), N166 (≠ V252), D198 (= D286), Y201 (= Y289), S231 (= S314), K232 (= K315), R240 (= R323)

Sites not aligning to the query:

1gd9A Crystall structure of pyrococcus protein-a1 (see paper)
28% identity, 27% coverage: 185:328/543 of query aligns to 101:245/388 of 1gd9A

query
sites
1gd9A

M

M

K

G

Q

L

N

S

G

A

L

F

V

L

E

K

R

D

G

G

D

E

K

E

V

V

A

L

I

I

G

P

L

T

P

P

V

A

F
|
F

T

V

P

S

Y

Y

-

A

-

P

-

A

-

V

-

I

-

L

-

A

-

G

-

K

-

P

I

V

E

E

I

V

P

P

E

T

L

Y

D

E

E

E

Y

-

G

-

L

-

T

D

E

E

V

F

A

R

I

L

N

N

A

V

D

D

P

-

A

-

K

-

G

-

W

-

Q

-

Y

-

P

-

D

-

A

-

E

E

L

L

D

K

K

K

L

Y

K

V

D

T

P

D

A

K

V

T

K

R

V

A

F

L

F

I

C

I

V

N

N

S

P

P

S

C

N
|
N

P

P

P

T

S

G

V

A

K

V

M

L

D

T

D

K

R

K

S

D

L

L

D

E

R

E

I

I

A

A

A

D

I

F

V

V

K

V

N

E

E

H

R

-

K

-

D

D

L

L

I

I

I

V

L

I

T

S

D
|
D

D

E

V
|
V

Y
|
Y

G

E

T

H

F

F

A

I

D

Y

N

D

F

D

R

A

S

R

L

H

F

Y

A

S

V

I

C

A

P

S

-

L

-

D

-

G

-

M

-

F

E

E

N

R

T

T

M

I

L

T

V

V

Y

N

S

G

F

F

S
|
S

K
|
K

Y

T

F

F

G

A

A

M

T

T

G

G

W

W

R
|
R

L

L

G

G

V

F

I

V

A

A

active site: K232 (= K315)
binding pyridoxal-5'-phosphate: F120 (= F204), N170 (= N256), D198 (= D286), V200 (= V288), Y201 (= Y289), S231 (= S314), K232 (= K315), R240 (= R323)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 94, 95, 96

5wmiA Arabidopsis thaliana prephenate aminotransferase mutant- t84v (see paper)
31% identity, 27% coverage: 194:337/543 of query aligns to 113:260/402 of 5wmiA

query
sites
5wmiA

G

G

D

D

K

E

V

V

A

I

I

I

G

P

L

A

P

P

V

Y

F

W

T

V

P

S

Y

Y

I

T

E

E

I

Q

P

A

E

R

L

L

D

A

E

D

Y

-

G

-

L

A

T

T

E

P

V

V

A

V

I

I

N

P

A

T

D

K

P

I

A

S

K

N

G

N

W

F

Q

L

Y

L

P

D

D

P

A

K

E

D

L

L

D

E

-

S

K

K

L

L

K

T

D

E

P

K

A

S

V

R

K

L

V

L

F

I

F

L

C

C

V

-

N

S

P

P

S

S

N
|
N

P

P

P

T

S

G

V

S

K

V

M

Y

D

P

D

K

R

S

S

L

L

L

D

E

R

E

I

I

A

A

A

R

I

I

V

I

K

A

N

K

E

H

R

P

K

R

D

-

L

L

I

L

I

V

L

L

T

S

D

D

D

E

V

I

Y

Y

G

E

-

H

-

I

T

I

F

Y

A

A

D

P

N

A

F

T

R

H

S

T

L

S

F

F

A

A

V

S

C

L

P

P

-

D

-

M

-

Y

E

E

N

R

T

T

M

L

L

T

V

V

Y

N

S

G

F

F

S

S

K
|
K

Y

A

F

F

G

A

A

M

T

T

G

G

W

W

R

R

L

L

G

G

V

Y

I

L

A

A

-

G

-

P

T

K

H

H

K

I

T

V

N

A

V

A

C

C

D

S

Q

K

binding 2-oxoglutaric acid: N173 (= N256), K236 (= K315)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 15, 36, 375

5wmhA Arabidopsis thaliana prephenate aminotransferase (see paper)
31% identity, 27% coverage: 194:337/543 of query aligns to 113:260/399 of 5wmhA

query
sites
5wmhA

G

G

D

D

K

E

V

V

A

I

I

I

G

P

L

A

P

P

V

Y

F
x
W

T

V

P

S

Y

Y

I

T

E

E

I

Q

P

A

E

R

L

L

D

A

E

D

Y

-

G

-

L

A

T

T

E

P

V

V

A

V

I

I

N

P

A

T

D

K

P

I

A

S

K

N

G

N

W

F

Q

L

Y

L

P

D

D

P

A

K

E

D

L

L

D

E

-

S

K

K

L

L

K

T

D

E

P

K

A

S

V

R

K

L

V

L

F

I

F

L

C

C

V

-

N

S

P

P

S

S

N
|
N

P

P

P

T

S

G

V

S

K

V

M

Y

D

P

D

K

R

S

S

L

L

L

D

E

R

E

I

I

A

A

A

R

I

I

V

I

K

A

N

K

E

H

R

P

K

R

D

-

L

L

I

L

I

V

L

L

T

S

D
|
D

D

E

V
x
I

Y
|
Y

G

E

-

H

-

I

T

I

F

Y

A

A

D

P

N

A

F

T

R

H

S

T

L

S

F

F

A

A

V

S

C

L

P

P

-

D

-

M

-

Y

E

E

N

R

T

T

M

L

L

T

V

V

Y

N

S

G

F

F

S
|
S

K
|
K

Y

A

F

F

G

A

A

M

T

T

G

G

W

W

R
|
R

L

L

G

G

V

Y

I

L

A

A

-

G

-

P

T

K

H

H

K

I

T

V

N

A

V

A

C

C

D

S

Q

K

binding pyridoxal-5'-phosphate: W123 (≠ F204), N173 (= N256), D202 (= D286), I204 (≠ V288), Y205 (= Y289), S235 (= S314), K236 (= K315), R244 (= R323)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 97, 98, 99

Q9C969 Aromatic aminotransferase ISS1; Methionine aminotransferase ISS1; Phenylalanine aminotransferase ISS1; Protein INDOLE SEVERE SENSITIVE 1; Protein REVERSAL OF SAV3 PHENOTYPE 1; Tryptophan aminotransferase ISS1; Tyrosine aminotransferase ISS1; EC 2.6.1.27; EC 2.6.1.5; EC 2.6.1.88 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
38% identity, 18% coverage: 233:328/543 of query aligns to 149:246/394 of Q9C969

query
sites
Q9C969

Y

Y

P

P

D

D

A

A

E

D

-

W

L

L

D

E

K

R

L

T

-

L

-

S

-

E

K

S

D

K

P

P

A

T

V

P

K

K

V

V

F

V

F

T

C

V

V

V

N

N

P

P

S

G

N

N

P

P

P

S

S
x
G

V

T

K

Y

M

V

D

P

D

E

R

P

S

L

L

L

D

K

R

R

I

I

A

A

A

Q

I

I

V

C

K

K

N

D

E

A

R

G

K

C

D

W

L

L

I

I

I

V

L

-

T

-

D

D

D

N

V

T

Y

Y

G

E

T

Y

F

F

A

M

D

Y

N

D

F

G

R

L

S

K

L

H

F

C

A
x
C

V

V

C

E

P

G

E

D

N

H

T

I

M

V

L

N

V

V

Y

F

S

S

F

F

S

S

K

K

Y

T

F

Y

G

G

A

M

T

M

G

G

W

W

R

R

L

L

G

G

V

Y

I

I

A

A

G179 (≠ S259) mutation to D: In vas1-2; suppressor of sav3 mutant plants leading to rescued hypocotyl elongation in response to shade and restored auxin biosynthetic pathway. Increases levels of auxin (IAA) and indole-3-pyruvic acid (3-IPA).
C219 (≠ A301) mutation to Y: In vas1-7; suppressor of sav3 mutant plants leading to rescued hypocotyl elongation in response to shade and restored auxin biosynthetic pathway.

Sites not aligning to the query:

66 P→L: In vas1-4; suppressor of sav3 mutant plants leading to rescued hypocotyl elongation in response to shade and restored auxin biosynthetic pathway.
77 L→F: In vas1-5; suppressor of sav3 mutant plants leading to rescued hypocotyl elongation in response to shade and restored auxin biosynthetic pathway.
269 A→T: In vas1-8; suppressor of sav3 mutant plants leading to rescued hypocotyl elongation in response to shade and restored auxin biosynthetic pathway.
362 R→W: In iss1-2; indole-dependent auxin (IAA) overproduction phenotypes including leaf epinasty and adventitious rooting.

P04694 Tyrosine aminotransferase; TAT; L-tyrosine:2-oxoglutarate aminotransferase; EC 2.6.1.5 from Rattus norvegicus (Rat) (see 2 papers)
27% identity, 35% coverage: 141:330/543 of query aligns to 116:295/454 of P04694

query
sites
P04694

L

L

A

S

I

S

S

R

E

E

D

E

V

V

V

A

R

S

H

Y

Y

Y

L

H

V

C

K

H

E

E

M

A

V

-

G

-

F

-

L

-

P

P

P

L

G

E

S

A

V

K

D

D

L

V

F

I

A

L

V

T

E

S

G

G

G

C

T

S

A

Q

A

A

M

I

T

E

Y

L

I

C

F

L

N

A

S

V

M

-

K

-

Q

-

N

-

G

-

L

L

V

A

E

N

R

P

G

G

D

Q

K

N

V

I

A

L

I

I

G

P

L

R

P

P

V

G

F

F

T

S

P

L

Y

Y

I

R

E

T

I

L

P

A

E

E

L

-

D

-

E

S

Y

M

G

G

L

I

T

E

E

V

V

K

A

L

I

Y

N

N

A

L

D

L

P

P

A

E

K

K

G

S

W

W

Q

E

Y

I

P

D

D

L

A

K

E

Q

L

L

D

E

K

S

L

L

K

I

D

D

P

E

A

K

V

T

K

A

V

C

F

L

F

V

C

V

V

N

N

N

P

P

S

S

N

N

P

P

P

C

S

G

V

S

K

V

M

F

D

S

D

K

R

R

S

H

L

L

D

Q

R

K

I

I

A

L

A

A

I

V

V

A

K

-

N

-

E

E

R

R

K

Q

D

C

L

V

I

P

I

I

L

L

T

A

D

D

D

E

V

I

Y

Y

G

G

-

D

-

M

T

V

F

F

A

S

D

D

N

C

F

K

R

Y

S

E

L

P

F

L

A

A

V

N

C

L

P

S

E

T

N

N

T

V

M

P

L

I

V

L

Y

S

S

C

-

G

F

L

S

A

K
|
K

Y

R

F

W

G

L

A

V

T

P

G

G

W

W

R

R

L

L

G

G

V

W

I

I

A

L

T

I

H

H

K280 (= K315) modified: N6-(pyridoxal phosphate)lysine

Sites not aligning to the query:

1 modified: N-acetylmethionine

5wmlA Arabidopsis thaliana prephenate aminotransferase mutant- k306a (see paper)
30% identity, 27% coverage: 194:337/543 of query aligns to 114:261/404 of 5wmlA

query
sites
5wmlA

G

G

D

D

K

E

V

V

A

I

I

I

G

P

L

A

P

P

V

Y

F
x
W

T

V

P

S

Y

Y

I

T

E

E

I

Q

P

A

E

R

L

L

D

A

E

D

Y

-

G

-

L

A

T

T

E

P

V

V

A

V

I

I

N

P

A

T

D

K

P

I

A

S

K

N

G

N

W

F

Q

L

Y

L

P

D

D

P

A

K

E

D

L

L

D

E

-

S

K

K

L

L

K

T

D

E

P

K

A

S

V

R

K

L

V

L

F

I

F

L

C

C

V

-

N

S

P

P

S

S

N
|
N

P

P

P

T

S

G

V

S

K

V

M

Y

D

P

D

K

R

S

S

L

L

L

D

E

R

E

I

I

A

A

A

R

I

I

V

I

K

A

N

K

E

H

R

P

K

R

D

-

L

L

I

L

I

V

L

L

T

S

D
|
D

D

E

V

I

Y
|
Y

G

E

-

H

-

I

T

I

F

Y

A

A

D

P

N

A

F

T

R

H

S

T

L

S

F

F

A

A

V

S

C

L

P

P

-

D

-

M

-

Y

E

E

N

R

T

T

M

L

L

T

V

V

Y

N

S

G

F

F

S
|
S

K

A

Y

A

F

F

G

A

A

M

T

T

G

G

W

W

R
|
R

L

L

G

G

V

Y

I

L

A

A

-

G

-

P

T

K

H

H

K

I

T

V

N

A

V

A

C

C

D

S

Q

K

binding glutamic acid: W124 (≠ F204)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: W124 (≠ F204), N174 (= N256), D203 (= D286), Y206 (= Y289), S236 (= S314), R245 (= R323)

Sites not aligning to the query:

binding glutamic acid: 15, 16, 37, 38, 100, 326, 376
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: 98, 99, 100, 103

5yhvB Crystal structure of an aminotransferase from mycobacterium tuberculosis
30% identity, 25% coverage: 192:325/543 of query aligns to 112:243/387 of 5yhvB

query
sites
5yhvB

E

D

R

A

G

G

D

D

K

R

V

V

A

A

I

M

G

A

L

S

P

P

V

G

F
x
Y

T

P

P

C

Y

Y

I

R

E

N

I

I

P

-

E

-

L

L

D

S

E

A

Y

L

G

G

L

C

T

E

E

V

V

V

A

E

I

I

N

P

A

C

D

G

P

P

A

Q

K

T

G

R

W

F

Q

Q

Y

-

P

P

D

T

A

A

E

Q

L

M

D

L

K

A

L

E

K

I

D

D

P

P

A

P

V

L

K

R

V

G

F

V

C

V

V

A

N

S

P

P

S

A

N
|
N

P

P

P

T

S

G

V

T

K

V

M

I

D

P

R

E

S

E

L

L

D

A

R

A

I

I

A

A

A

S

I

W

V

C

K

-

N

-

E

D

R

A

K

S

D

D

L

V

I

R

I

L

L

I

T

S

D
|
D

D

E

V
|
V

Y
|
Y

G
x
H

T

G

F

L

A

V

D

Y

N

Q

F

G

-

A

-

P

-

Q

R
x
T

S

S

L

C

F

A

A

W

V

Q

C

T

P

S

E

R

N

N

T

A

M

V

L

V

V

V

Y
x
N

S
|
S

F

F

S
|
S

K
|
K

Y

Y

F

Y

G

A

A

M

T

T

G

G

W

W

R
|
R

L

L

G

G

binding 2-oxoglutaric acid: H205 (≠ G290), T215 (≠ R297), N229 (≠ Y311)
binding pyridoxal-5'-phosphate: Y124 (≠ F204), N173 (= N256), D201 (= D286), V203 (= V288), Y204 (= Y289), S230 (= S312), S232 (= S314), K233 (= K315), R241 (= R323)

Sites not aligning to the query:

5yhvA Crystal structure of an aminotransferase from mycobacterium tuberculosis
30% identity, 25% coverage: 192:325/543 of query aligns to 119:250/394 of 5yhvA

query
sites
5yhvA

E

D

R

A

G

G

D

D

K

R

V

V

A

A

I

M

G

A

L

S

P

P

V

G

F
x
Y

T

P

P

C

Y

Y

I

R

E

N

I

I

P

-

E

-

L

L

D

S

E

A

Y

L

G

G

L

C

T

E

E

V

V

V

A

E

I

I

N

P

A

C

D

G

P

P

A

Q

K

T

G

R

W

F

Q

Q

Y

-

P

P

D

T

A

A

E

Q

L

M

D

L

K

A

L

E

K

I

D

D

P

P

A

P

V

L

K

R

V

G

F

V

C

V

V

A

N

S

P

P

S

A

N
|
N

P

P

P

T

S

G

V

T

K

V

M

I

D

P

R

E

S

E

L

L

D

A

R

A

I

I

A

A

A

S

I

W

V

C

K

-

N

-

E

D

R

A

K

S

D

D

L

V

I

R

I

L

L

I

T

S

D

D

D

E

V

V

Y
|
Y

G
x
H

T

G

F

L

A

V

D

Y

N

Q

F

G

-

A

-

P

-

Q

R

T

S

S

L

C

F

A

A

W

V

Q

C

T

P

S

E

R

N

N

T

A

M

V

L

V

V

V

Y

N

S
|
S

F

F

S
|
S

K
|
K

Y

Y

F

Y

G

A

A

M

T

T

G

G

W

W

R
|
R

L

L

G

G

binding glutamic acid: H212 (≠ G290)
binding pyridoxal-5'-phosphate: Y131 (≠ F204), N180 (= N256), Y211 (= Y289), S237 (= S312), S239 (= S314), K240 (= K315), R248 (= R323)

Sites not aligning to the query:

P96847 Valine--pyruvate aminotransferase; Alanine--valine transaminase; EC 2.6.1.66 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
30% identity, 25% coverage: 192:325/543 of query aligns to 113:244/388 of P96847

query
sites
P96847

E

D

R

A

G

G

D

D

K

R

V

V

A

A

I

M

G

A

L

S

P

P

V

G

F

Y

T

P

P

C

Y

Y

I

R

E

N

I

I

P

-

E

-

L

L

D

S

E

A

Y

L

G

G

L

C

T

E

E

V

V

V

A

E

I

I

N

P

A

C

D

G

P

P

A

Q

K

T

G

R

W

F

Q

Q

Y

-

P

P

D

T

A

A

E

Q

L

M

D

L

K

A

L

E

K

I

D

D

P

P

A

P

V

L

K

R

V

G

F

V

C

V

V

A

N

S

P

P

S

A

N

N

P

P

P

T

S

G

V

T

K

V

M

I

D

P

R

E

S

E

L

L

D

A

R

A

I

I

A

A

A

S

I

W

V

C

K

-

N

-

E

D

R

A

K

S

D

D

L

V

I

R

I

L

L

I

T

S

D

D

D

E

V

V

Y

Y

G

H

T

G

F

L

A

V

D

Y

N

Q

F

G

-

A

-

P

-

Q

R

T

S

S

L

C

F

A

A

W

V

Q

C

T

P

S

E

R

N

N

T

A

M

V

L

V

V

V

Y

N

S

S

F

F

S

S

K
|
K

Y

Y

F

Y

G

A

A

M

T

T

G

G

W

W

R

R

L

L

G

G

K234 (= K315) modified: N6-(pyridoxal phosphate)lysine

1o4sB Crystal structure of aspartate aminotransferase (tm1255) from thermotoga maritima at 1.90 a resolution (see paper)
27% identity, 30% coverage: 177:341/543 of query aligns to 106:269/384 of 1o4sB

query
sites
1o4sB

A
|
A

M
x
K

T

Q

Y

A

I

L

F

F

N

N

S

A

M

F

K

M

Q

-

N

-

G

A

L

L

V

L

E

D

R

P

G

G

D

D

K

E

V

V

A

I

I

V

G

F

L

S

P

P

V

V

F
x
W

T

V

P

S

Y

Y

I

I

E

-

I

-

P

P

E

Q

L

I

D

I

E

L

Y

A

G

G

L

G

T

T

E

V

V

N

A

V

I

V

N

E

A

T

D

F

P

M

A

S

K

K

G

N

W

F

Q

Q

Y

P

P

S

D

L

A

E

E

E

L

V

D

E

K

G

L

L

K

L

D

V

P

G

A

K

V

T

K

K

V

A

F

V

F

L

C

I

V

N

N

S

P

P

S

N

N
|
N

P

P

P

T

S

G

V

V

K

V

M

Y

D

R

R

E

S

F

L

L

D

E

R

G

I

L

A

V

A

R

I

L

V

A

K

K

N

-

E

-

R

K

K

R

D

N

L

F

I

Y

I

I

L

I

T

S

D
|
D

D

E

V
|
V

Y
|
Y

G

D

T

S

-

L

-

V

F

Y

A

T

D

D

N

E

F

F

R

T

S

S

L

I

F

L

A

D

V

V

C

S

P

E

E

G

N

F

T

D

M

R

L

I

V

V

Y

Y

-

I

-

N

S

G

F

F

S
|
S

K
|
K

Y

S

F

H

G

S

A

M

T

T

G

G

W

W

R
|
R

L

V

G

G

-

Y

V

L

I

I

A

S

T

S

H

E

K

K

T

V

N

A

V

T

C

A

D

V

Q

S

R

K

I

I

A

Q

A

S

active site: W131 (≠ F204), D209 (= D286), V211 (= V288), K242 (= K315)
binding pyridoxal-5'-phosphate: A106 (= A177), K107 (≠ M178), W131 (≠ F204), N181 (= N256), D209 (= D286), V211 (= V288), Y212 (= Y289), S241 (= S314), K242 (= K315), R250 (= R323)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 105

P17735 Tyrosine aminotransferase; TAT; L-tyrosine:2-oxoglutarate aminotransferase; EC 2.6.1.5 from Homo sapiens (Human) (see paper)
28% identity, 26% coverage: 190:330/543 of query aligns to 155:295/454 of P17735

query
sites
P17735

L

L

V

A

E

N

R

P

G

G

D

Q

K

N

V

I

A

L

I

V

G

P

L

R

P

P

V

G

F

F

T

S

P

L

Y

Y

I

K

E

T

I

L

P

A

E

E

L

-

D

-

E

S

Y

M

G

G

L

I

T

E

E

V

V

K

A

L

I

Y

N

N

A

L

D

L

P

P

A

E

K

K

G

S

W

W

Q

E

Y

I

P

D

D

L

A

K

E

Q

L

L

D

E

K

Y

L

L

K

I

D

D

P

E

A

K

V

T

K

A

V

C

F

L

F

I

C

V

V

N

N

N

P

P

S

S

N

N

P

P

P

C

S

G

V

S

K

V

M

F

D

S

D

K

R

R

S

H

L

L

D

Q

R

K

I

I

A

L

A

A

I

V

V

A

K

A

N

-

E

-

R

R

K

Q

D

C

L

V

I

P

I

I

L

L

T

A

D

D

D

E

V

I

Y

Y

G

G

-

D

-

M

T

V

F

F

A

S

D

D

N

C

F

K

R

Y

S

E

L

P

F

L

A

A

V

T

C

L

P

S

E

T

N

D

T

V

M

P

L

I

V

L

Y

S

S

C

-

G

F

L

S

A

K
|
K

Y

R

F

W

G

L

A

V

T

P

G

G

W

W

R

R

L

L

G

G

V

W

I

I

A

L

T
x
I

H

H

K280 (= K315) modified: N6-(pyridoxal phosphate)lysine
I294 (≠ T329) mutation to A: Reduced catalytic activity.

3dydA Human tyrosine aminotransferase
28% identity, 26% coverage: 190:330/543 of query aligns to 99:239/388 of 3dydA

query
sites
3dydA

L

L

V

A

E

N

R

P

G

G

D

Q

K

N

V

I

A

L

I

V

G

P

L

R

P

P

V

G

F
|
F

T

S

P

L

Y

Y

I

K

E

T

I

L

P

A

E

E

L

-

D

-

E

S

Y

M

G

G

L

I

T

E

E

V

V

K

A

L

I

Y

N

N

A

L

D

L

P

P

A

E

K

K

G

S

W

W

Q

E

Y

I

P

D

D

L

A

K

E

Q

L

L

D

E

K

Y

L

L

K

I

D

D

P

E

A

K

V

T

K

A

V

C

F

L

F

I

C

V

V
x
N

N

N

P

P

S

S

N
|
N

P

P

P

C

S

G

V

S

K

V

M

F

D

S

D

K

R

R

S

H

L

L

D

Q

R

K

I

I

A

L

A

A

I

V

V

A

K

A

N

-

E

-

R

R

K

Q

D

C

L

V

I

P

I

I

L

L

T

A

D
|
D

D

E

V
x
I

Y
|
Y

G

G

-

D

-

M

T

V

F

F

A

S

D

D

N

C

F

K

R

Y

S

E

L

P

F

L

A

A

V

T

C

L

P

S

E

T

N

D

T

V

M

P

L

I

V

L

Y

S

S

C

-

G

F

L

S

A

K
|
K

Y

R

F

W

G

L

A

V

T

P

G

G

W

W

R
|
R

L

L

G

G

V

W

I

I

A

L

T

I

H

H

active site: F113 (= F204), D191 (= D286), I193 (≠ V288)
binding pyridoxal-5'-phosphate: F113 (= F204), N159 (≠ V252), N163 (= N256), D191 (= D286), I193 (≠ V288), Y194 (= Y289), K224 (= K315), R232 (= R323)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 87, 88, 89

5verA Mouse kynurenine aminotransferase iii, re-refinement of the PDB structure 3e2z (see paper)
24% identity, 45% coverage: 79:325/543 of query aligns to 4:250/410 of 5verA

query
sites
5verA

R

R

L

I

E
|
E

G

G

I

L

E

D

G

S

R

N

F

V

E

W

R

V

Y

E

V

F

S

T

E

K

H

L

R

A

D

A

Q

D

E

P

G

S

V

V

F

N

L

L

G

G

R

Q

A

G

L

F

-

P

-

D

-

I

-

S

-

P

S

S

Y

Y

V

V

R

K

D

E

Q

E

L

L

G

-

L

-

S

S

G

K

S

A

G

A

F

F

L

I

H

D

E

N

M

M

V

N

E

Q

G

Y

V

T

L

R

G

G

A

F

N

G

Y

H

P

P

V

A

P

L

P

V

R

K

M

A

L

L

A

S

I

C

S

-

E

-

D

-

V

-

R

-

H

-

Y

-

L

L

V

Y

K

G

E

K

M

I

V

Y

G

Q

G

R

F

Q

L

I

P

D

P

P

G

N

S

E

V

E

D

I

L

L

F

V

A

A

V

V

E

-

G

-

T

-

A
x
G

A
|
A

M
x
Y

T

G

Y

S

I

L

F

F

N

N

S

S

M

I

K

Q

Q

-

N

-

G

G

L

L

V

V

E

D

R

P

G

G

D

D

K

E

V

V

A

I

I

I

G

M

L

V

P

P

V

F

F
x
Y

T

D

P

C

Y

Y

I

E

E

P

I

M

P

V

E

R

L

M

D

A

E

G

Y

A

G

V

L

P

T

V

E

F

V

I

A

P

I

L

N

R

A

S

D

K

P

P

A

T

K

D

G

G

-

M

-

K

-

W

-

T

-

S

-

D

W

W

Q

T

Y

F

P

D

D

P

A

R

E

E

L

L

D

E

K

S

L

K

K

F

D

S

P

S

A

K

V

T

K

K

V

A

F

I

C

L

V
x
N

N

T

P

P

S

H

N
|
N

P

P

P

L

S

G

V

K

K

V

M

Y

D

T

D

R

R

Q

S

E

L

L

D

Q

R

V

I

I

A

A

A

D

I

L

V

C

K

V

N

-

E

-

R

K

K

H

D

D

L

T

I

L

I

C

L

I

T

S

D
|
D

D

E

V
|
V

Y
|
Y

G

E

-

W

-

L

T

V

F

Y

A

T

D

G

N

H

F

T

R

H

S

V

L

K

F

I

A

A

V

T

C

L

P

P

-

G

-

M

-

W

E

E

N

R

T

T

M

I

L

T

V

I

Y

G

S
|
S

F

A

S

G

K
|
K

Y

T

F

F

G

S

A

V

T

T

G

G

W

W

R
x
K

L

L

G

G

active site: Y119 (≠ F204), D206 (= D286), V208 (= V288), K240 (= K315)
binding calcium ion: E6 (= E81)
binding pyridoxal-5'-phosphate: G93 (≠ A176), A94 (= A177), Y95 (≠ M178), N174 (≠ V252), N178 (= N256), D206 (= D286), Y209 (= Y289), S237 (= S312), K240 (= K315), K248 (≠ R323)

Sites not aligning to the query:

binding calcium ion: 1

5vepA Mouse kynurenine aminotransferase iii, re-refinement of the PDB structure 3e2f (see paper)
24% identity, 45% coverage: 79:325/543 of query aligns to 4:250/410 of 5vepA

query
sites
5vepA

R

R

L

I

E
|
E

G

G

I

L

E

D

G

S

R

N

F

V

E

W

R

V

Y

E

V

F

S

T

E

K

H

L

R

A

D

A

Q

D

E

P

G

S

V

V

F

N

L

L

G

G

R

Q

A

G

L

F

-

P

-

D

-

I

-

S

-

P