SitesBLAST
Comparing AZOBR_RS31000 FitnessBrowser__azobra:AZOBR_RS31000 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6x9lA Crystal structure of aldehyde dehydrogenasE C (aldc) mutant (c291a) from pseudomonas syringae in complexed with NAD+ and octanal (see paper)
51% identity, 100% coverage: 2:478/479 of query aligns to 9:483/485 of 6x9lA
- active site: N154 (= N149), E252 (= E247), A286 (≠ C281), E462 (= E457)
- binding nicotinamide-adenine-dinucleotide: I150 (= I145), T151 (= T146), W153 (= W148), N154 (= N149), Q159 (= Q154), K177 (= K172), E180 (= E175), G210 (= G205), P211 (≠ A206), G214 (= G209), T229 (= T224), G230 (= G225), S231 (= S226), E252 (= E247), L253 (= L248), A286 (≠ C281), E386 (= E381), F388 (= F383), F451 (= F446)
- binding octanal: W155 (= W150), S285 (= S280)
3ty7B Crystal structure of aldehyde dehydrogenase family protein from staphylococcus aureus
43% identity, 99% coverage: 1:474/479 of query aligns to 2:451/454 of 3ty7B
3b4wA Crystal structure of mycobacterium tuberculosis aldehyde dehydrogenase complexed with NAD+
41% identity, 98% coverage: 5:472/479 of query aligns to 6:475/483 of 3b4wA
- active site: N154 (= N149), K177 (= K172), E251 (= E247), C285 (= C281), E384 (= E381), E460 (= E457)
- binding nicotinamide-adenine-dinucleotide: I150 (= I145), V151 (≠ T146), W153 (= W148), N154 (= N149), K177 (= K172), I210 (≠ A206), G213 (= G209), T228 (= T224), G229 (= G225), S230 (= S226), V233 (≠ A229), E236 (≠ S232), E251 (= E247), L252 (= L248), C285 (= C281), E384 (= E381), F386 (= F383)
5gtlA NADPH complex structure of aldehyde dehydrogenase from bacillus cereus
38% identity, 98% coverage: 6:475/479 of query aligns to 18:489/491 of 5gtlA
- active site: N165 (= N149), K188 (= K172), E263 (= E247), C297 (= C281), E394 (= E381), E471 (= E457)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I161 (= I145), P163 (= P147), K188 (= K172), A190 (≠ S174), E191 (= E175), Q192 (≠ F176), G221 (= G205), G225 (= G209), G241 (= G225), S242 (= S226), T245 (≠ A229), L264 (= L248), C297 (= C281), E394 (= E381), F396 (= F383)
5gtkA NAD+ complex structure of aldehyde dehydrogenase from bacillus cereus
38% identity, 98% coverage: 6:475/479 of query aligns to 18:489/491 of 5gtkA
- active site: N165 (= N149), K188 (= K172), E263 (= E247), C297 (= C281), E394 (= E381), E471 (= E457)
- binding nicotinamide-adenine-dinucleotide: I161 (= I145), I162 (≠ T146), P163 (= P147), W164 (= W148), K188 (= K172), E191 (= E175), G221 (= G205), G225 (= G209), A226 (≠ P210), F239 (≠ L223), G241 (= G225), S242 (= S226), T245 (≠ A229), Y248 (≠ S232), L264 (= L248), C297 (= C281), Q344 (= Q328), R347 (= R331), E394 (= E381), F396 (= F383)
4neaA 1.90 angstrom resolution crystal structure of betaine aldehyde dehydrogenase (betb) from staphylococcus aureus in complex with NAD+ and bme-free cys289 (see paper)
37% identity, 99% coverage: 4:475/479 of query aligns to 17:494/505 of 4neaA
- active site: N166 (= N149), K189 (= K172), E264 (= E247), C298 (= C281), E399 (= E381), E476 (= E457)
- binding nicotinamide-adenine-dinucleotide: P164 (= P147), K189 (= K172), E192 (= E175), G222 (= G205), G226 (= G209), G242 (= G225), G243 (≠ S226), T246 (≠ A229), H249 (≠ S232), I250 (≠ V233), C298 (= C281), E399 (= E381), F401 (= F383)
4pxlA Structure of zm aldh2-3 (rf2c) in complex with NAD (see paper)
39% identity, 97% coverage: 6:472/479 of query aligns to 7:476/486 of 4pxlA
- active site: N154 (= N149), K177 (= K172), E253 (= E247), C287 (= C281), E384 (= E381), D461 (≠ E457)
- binding nicotinamide-adenine-dinucleotide: I150 (= I145), V151 (≠ T146), P152 (= P147), W153 (= W148), K177 (= K172), E180 (= E175), G210 (= G205), G214 (= G209), A215 (≠ P210), F228 (≠ L223), G230 (= G225), S231 (= S226), V234 (≠ A229), E253 (= E247), G255 (= G249), C287 (= C281), Q334 (= Q328), K337 (≠ R331), E384 (= E381), F386 (= F383)
2d4eC Crystal structure of the hpcc from thermus thermophilus hb8
39% identity, 97% coverage: 8:472/479 of query aligns to 30:501/515 of 2d4eC
- active site: N173 (= N149), K196 (= K172), E271 (= E247), C305 (= C281), E409 (= E381), E486 (= E457)
- binding nicotinamide-adenine-dinucleotide: I169 (= I145), T170 (= T146), P171 (= P147), W172 (= W148), K196 (= K172), A198 (≠ S174), G229 (= G205), G233 (= G209), A234 (≠ P210), T248 (= T224), G249 (= G225), E250 (≠ S226), T253 (≠ A229), E271 (= E247), L272 (= L248), C305 (= C281), E409 (= E381), F411 (= F383), F475 (= F446)
O24174 Betaine aldehyde dehydrogenase 1; OsBADH1; EC 1.2.1.8 from Oryza sativa subsp. japonica (Rice) (see paper)
37% identity, 98% coverage: 5:472/479 of query aligns to 10:487/505 of O24174
- N164 (= N149) mutation to A: Slightly reduced affinity for NAD, 6-fold enhanced affinity for both gamma-4-aminobutyraldehyde (GAB-ald) and betaine aldehyde (Bet-ald), but 2-fold decrease in catalytic efficiency.
- W172 (≠ C157) mutation to A: Slightly reduced affinity for NAD, enhanced affinity for both betaine aldehyde (Bet-ald) (10-fold) and gamma-4-aminobutyraldehyde (GAB-ald) (2-fold).; mutation to F: Slightly reduced affinity for NAD, but 6-fold enhanced affinity for both gamma-4-aminobutyraldehyde (GAB-ald) and betaine aldehyde (Bet-ald) and 2-fold increase in catalytic efficiency towards GAB-ald.
Q56YU0 Aldehyde dehydrogenase family 2 member C4; ALDH1a; Protein REDUCED EPIDERMAL FLUORESCENCE 1; EC 1.2.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
37% identity, 97% coverage: 6:472/479 of query aligns to 21:491/501 of Q56YU0