SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AZOBR_RS31120 FitnessBrowser__azobra:AZOBR_RS31120 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P12995 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; Diaminopelargonic acid synthase; EC 2.6.1.62 from Escherichia coli (strain K12) (see 4 papers)
41% identity, 99% coverage: 2:429/434 of query aligns to 3:420/429 of P12995

query
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P12995

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Y17 (≠ F16) mutation to F: Severely reduces the aminotransferase activity.
W52 (= W51) binding
GS 112:113 (= GS 111:112) binding
Y144 (= Y144) mutation to F: Severely reduces the aminotransferase activity.
D147 (= D147) mutation to N: Loss of aminotransferase activity.
D245 (= D248) binding
R253 (= R256) mutation to A: Has only a small effect on the rate of reaction with DAPA.; mutation to K: Increases aminotransferase activity toward SAM.; mutation to M: Loss of aminotransferase activity.; mutation to Q: Increases aminotransferase activity toward SAM.
K274 (= K277) binding ; modified: N6-(pyridoxal phosphate)lysine
G307 (= G311) binding
PT 308:309 (≠ HS 312:313) binding
R391 (= R400) binding ; mutation to A: Reduces aminotransferase activity.

6ed7A Crystal structure of 7,8-diaminopelargonic acid synthase bound to inhibitor mac13772
41% identity, 99% coverage: 2:429/434 of query aligns to 3:420/429 of 6ed7A

query
sites
6ed7A

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active site: Y17 (≠ F16), Y144 (= Y144), D245 (= D248), K274 (= K277)
binding 2-[(2-nitrophenyl)sulfanyl]acetohydrazide: Y17 (≠ F16), W52 (= W51), W52 (= W51), Y144 (= Y144), D147 (= D147), A217 (= A220), K274 (= K277), R391 (= R400), F393 (≠ L402), F393 (≠ L402)
binding pyridoxal-5'-phosphate: G112 (= G111), S113 (= S112), Y144 (= Y144), H145 (= H145), D245 (= D248), I247 (≠ V250), K274 (= K277)

1dtyA Crystal structure of adenosylmethionine-8-amino-7-oxonanoate aminotransferase with pyridoxal phosphate cofactor.
41% identity, 99% coverage: 2:429/434 of query aligns to 3:420/429 of 1dtyA

query
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1dtyA

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M

L

G

G

T

A

I

I

A

G

A

V

I

V

E

E

V

T

T

T

D

H

A

P

Q

V

G

N

Y

M

T

A

A

A

A

-

V

-

G

-

Q

-

T

-

L

L

K

Q

R

K

F

F

L

V

E

E

R

Q

G

G

L

V

L

W

L

I

R

R

P

P

L

F

G

G

P

K

V

L

I

I

Y
|
Y

L

L

L

M

P

P

Y

Y

C

I

V

I

T

L

D

P

G

Q

Q

Q

L

L

D

Q

R

R

A

L

Y

T

A

A

I

V

R

N

D

R

A

A

active site: Y17 (≠ F16), Y144 (= Y144), E211 (= E214), D245 (= D248), A248 (≠ M251), K274 (= K277), Y398 (= Y407)
binding pyridoxal-5'-phosphate: G112 (= G111), S113 (= S112), Y144 (= Y144), H145 (= H145), D245 (= D248), I247 (≠ V250), K274 (= K277)

1qj3A Crystal structure of 7,8-diaminopelargonic acid synthase in complex with 7-keto-8-aminopelargonic acid (see paper)
41% identity, 99% coverage: 2:429/434 of query aligns to 3:408/416 of 1qj3A

query
sites
1qj3A

T

T

D

D

T

D

V

L

S

A

L

F

D

D

Q

Q

R

R

H

H

V

I

W

L

H

H

P

P

F
x
Y

T

T

Q

-

A

S

Q

M

T

T

A

S

P

P

E

L

P

P

L

V

-

Y

A

P

V

V

T

V

H

S

G

A

K

E

G

G

A

C

S

E

L

L

F

I

T

L

E

S

D

D

G

G

R

R

E

R

I

L

L

V

D

D

L

G

I

M

S

S

W
|
W

W

W

V

A

N

A

L

I

H

H

G

G

H

Y

A

N

H

H

P

P

A

Q

V

L

A

N

G

A

A

A

I

M

A

K

E

S

Q

Q

A

I

H

D

R

A

L

M

E

S

Q

H

V

V

I

M

F

F

A

G

D

G

F

I

T

T

H

H

S

A

P

P

A

A

A

I

R

E

L

L

A

C

A

R

R

K

L

L

A

V

E

A

V

M

L

T

P

P

G

Q

G

P

L

L

G

E

R

C

V

V

F

F

Y

L

S

A

D

D

N

S

G
|
G

S
|
S

T

V

A

A

V

V

E

E

V

V

A

A

L

M

K

K

L

M

A

A

W

L

Q

Q

Y

Y

W

W

R

Q

N

A

K

K

G

G

E

E

G

A

Q

-

R

R

R

Q

R

R

F

F

L

L

A

T

F

F

E

R

G

N

S

G

Y
|
Y

H
|
H

G
|
G

D

D

T

T

F

F

G

G

A

A

M

M

A

S

A

V

G

C

V

D

G

P

S

D

G

N

F

-

Y

-

A

-

P

-

F

L

Q

P

E

E

L

N

L

L

F

F

A

A

V

P

D

A

R

P

M

Q

P

S

Y

R

P

M

A

G

T

E

W

W

D

D

G

E

D

R

P

D

E

M

V

V

E

G

A

-

K

-

E

-

A

-

L

-

D

-

W

-

L

F

D

A

R

R

W

L

L

M

A

A

T

A

N

H

G

R

A

H

E

E

L

I

V

A

A

A

V

V

I

I

E
|
E

P

P

L

I

V

V

Q

Q

G

G

A

A

S

G

G

G

M

M

R

R

F

M

C

Y

R

H

P

P

E

E

F

W

L

L

R

K

A

R

M

I

A

R

A

K

R

I

V

C

R

D

A

R

A

E

G

G

G

I

L

L

V

L

I

I

F

A

D
|
D

E

E

V
x
I

M
x
A

T

T

G

G

F

F

G

G

R

R

T

T

G

G

A

K

L

L

F

F

A

A

S

C

Q

E

K

H

A

A

G

E

V

I

A

A

P

P

D

D

L

I

I

L

C

C

L

L

S

G

K
|
K

G

A

L

L

T

T

G

G

F

T

L

M

P

T

L

L

S

S

V

A

T

T

A

L

C

T

G

T

A

R

S

E

I

V

Y

A

E

E

A

T

F

I

L

S

G

N

A

G

G

G

F

-

D

-

R

-

A

C

F

F

A

M

H

H

G

G

H

P

S

T

F

F

T

M

A

G

N

N

P

P

L

L

G

A

C

C

A

A

L

N

A

A

S

S

L

L

E

A

L

I

T

L

T

E

S

S

A

G

E

D

T

W

T

Q

A

Q

N

Q

L

V

A

A

R

D

I

I

E

E

T

V

R

Q

H

L

R

R

A

E

A

Q

I

L

A

A

D

P

L

A

S

R

G

D

H

A

P

E

K

M

L

V

S

A

R

D

G

V

R

R

V

V

M

L

G

G

T

A

I

I

A

G

A

V

I

V

E

E

V

T

T

T

D

H

A

P

Q

V

G

N

Y

M

T

A

A

A

A

-

V

-

G

-

Q

-

T

-

L

L

K

Q

R

K

F

F

L

V

E

E

R

Q

G

G

L

V

L

W

L

I

R
|
R

P

P

L
x
F

G

G

P

K

V

L

I

I

Y
|
Y

L

L

L

M

P

P

Y

Y

C

I

V

I

T

L

D

P

G

Q

Q

Q

L

L

D

Q

R

R

A

L

Y

T

A

A

I

V

R

N

D

R

A

A

active site: Y17 (≠ F16), Y144 (= Y144), E201 (= E214), D235 (= D248), A238 (≠ M251), K264 (= K277), Y386 (= Y407)
binding 7-keto-8-aminopelargonic acid: Y17 (≠ F16), W52 (= W51), Y144 (= Y144), K264 (= K277), R379 (= R400), F381 (≠ L402)
binding pyridoxal-5'-phosphate: G112 (= G111), S113 (= S112), Y144 (= Y144), H145 (= H145), G146 (= G146), D235 (= D248), I237 (≠ V250), A238 (≠ M251), K264 (= K277)

1mlzA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the trans-isomer of amiclenomycin. (see paper)
41% identity, 99% coverage: 2:429/434 of query aligns to 3:419/427 of 1mlzA

query
sites
1mlzA

T

T

D

D

T

D

V

L

S

A

L

F

D

D

Q

Q

R

R

H

H

V

I

W

L

H

H

P

P

F
x
Y

T

T

Q

-

A

S

Q

M

T

T

A

S

P

P

E

L

P

P

L

V

-

Y

A

P

V

V

T

V

H

S

G

A

K

E

G

G

A

C

S

E

L

L

F

I

T

L

E

S

D

D

G

G

R

R

E

R

I

L

L

V

D

D

L

G

I

M

S

S

W
|
W

V

A

N

A

L

I

H

H

G

G

H

Y

A

N

H

H

P

P

A

Q

V

L

A

N

G

A

A

A

I

M

A

K

E

S

Q

Q

A

I

H

D

R

A

L

M

E

S

Q

H

V

V

I

M

F

F

A

G

D

G

F

I

T

T

H

H

S

A

P

P

A

A

A

I

R

E

L

L

A

C

A

R

R

K

L

L

A

V

E

A

V

M

L

T

P

P

G

Q

G

P

L

L

G

E

R

C

V

V

F

F

Y

L

S

A

D

D

N

S

G
|
G

S
|
S

T

V

A

A

V

V

E

E

V

V

A

A

L

M

K

K

L

M

A

A

W

L

Q

Q

Y

Y

W

W

R

Q

N

A

K

K

G

G

E

E

G

A

Q

-

R

R

R

Q

R

R

F

F

L

L

A

T

F

F

E

R

G

N

S

G

Y
|
Y

H
|
H

G

G

D

D

T

T

F

F

G

G

A

A

M

M

A

S

A

V

-

C

-

D

-

P

-

D

-

N

G

S

V

M

G

H

S

S

G

L

F

W

Y

K

A

G

P

Y

F

L

Q

P

E

E

L

N

L

L

F

F

A

A

V

P

D

A

R

P

M

Q

P

S

Y

R

P

M

A

G

T

E

W

W

D

D

G

E

D

R

P

D

E

M

V

V

E

G

A

-

K

-

E

-

A

-

L

-

D

-

W

-

L

F

D

A

R

R

W

L

L

M

A

A

T

A

N

H

G

R

A

H

E

E

L

I

V

A

A

A

V

V

I

I

E
|
E

P

P

L

I

V

V

Q

Q

G

G

A

A

S

G

G

G

M

M

R

R

F

M

C

Y

R

H

P

P

E

E

F

W

L

L

R

K

A

R

M

I

A

R

A

K

R

I

V

C

R

D

A

R

A

E

G

G

G

I

L

L

V

L

I

I

F

A

D
|
D

E

E

V
x
I

M
x
A

T

T

G

G

F

F

G

G

R

R

T

T

G

G

A

K

L

L

F

F

A

A

S

C

Q

E

K

H

A

A

G

E

V

I

A

A

P

P

D

D

L

I

I

L

C

C

L

L

S

G

K
|
K

G

A

L

L

T

T

G

G

F

T

L

M

P

T

L

L

S

S

V

A

T

T

A

L

C

T

G

T

A

R

S

E

I

V

Y

A

E

E

A

T

F

-

L

I

G

S

A

N

G

G

F

E

D

A

R

G

A

C

F

F

A

M

H

H

G

G

H
x
P

S
x
T

F

F

T

M

A

G

N

N

P

P

L

L

G

A

C

C

A

A

L

N

A

A

S

S

L

L

E

A

L

I

T

L

T

E

S

S

A

G

E

D

T

W

T

Q

A

Q

N

Q

L

V

A

A

R

D

I

I

E

E

T

V

R

Q

H

L

R

R

A

E

A

Q

I

L

A

A

D

P

L

A

S

R

G

D

H

A

P

E

K

M

L

V

S

A

R

D

G

V

R

R

V

V

M

L

G

G

T

A

I

I

A

G

A

V

I

V

E

E

V

T

T

T

D

H

A

P

Q

V

G

N

Y

M

T

A

A

A

A

-

V

-

G

-

Q

-

T

-

L

L

K

Q

R

K

F

F

L

V

E

E

R

Q

G

G

L

V

L

W

L

I

R
|
R

P

P

L
x
F

G

G

P

K

V

L

I

I

Y
|
Y

L

L

L

M

P

P

Y

Y

C

I

V

I

T

L

D

P

G

Q

Q

Q

L

L

D

Q

R

R

A

L

Y

T

A

A

I

V

R

N

D

R

A

A

active site: Y17 (≠ F16), Y144 (= Y144), E210 (= E214), D244 (= D248), A247 (≠ M251), K273 (= K277), Y397 (= Y407)
binding pyridoxal-5'-phosphate: G112 (= G111), S113 (= S112), Y144 (= Y144), H145 (= H145), D244 (= D248), I246 (≠ V250), K273 (= K277), P307 (≠ H312), T308 (≠ S313)
binding trans-amiclenomycin: W52 (= W51), W53 (= W52), Y144 (= Y144), K273 (= K277), R390 (= R400), F392 (≠ L402)

1mlyA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the cis isomer of amiclenomycin (see paper)
41% identity, 99% coverage: 2:429/434 of query aligns to 3:419/427 of 1mlyA

query
sites
1mlyA

T

T

D

D

T

D

V

L

S

A

L

F

D

D

Q

Q

R

R

H

H

V

I

W

L

H

H

P

P

F
x
Y

T

T

Q

-

A

S

Q

M

T

T

A

S

P

P

E

L

P

P

L

V

-

Y

A

P

V

V

T

V

H

S

G

A

K

E

G

G

A

C

S

E

L

L

F

I

T

L

E

S

D

D

G

G

R

R

E

R

I

L

L

V

D

D

L

G

I

M

S

S

W
|
W

V

A

N

A

L

I

H

H

G

G

H

Y

A

N

H

H

P

P

A

Q

V

L

A

N

G

A

A

A

I

M

A

K

E

S

Q

Q

A

I

H

D

R

A

L

M

E

S

Q

H

V

V

I

M

F

F

A

G

D

G

F

I

T

T

H

H

S

A

P

P

A

A

A

I

R

E

L

L

A

C

A

R

R

K

L

L

A

V

E

A

V

M

L

T

P

P

G

Q

G

P

L

L

G

E

R

C

V

V

F

F

Y

L

S

A

D

D

N

S

G
|
G

S
|
S

T

V

A

A

V

V

E

E

V

V

A

A

L

M

K

K

L

M

A

A

W

L

Q

Q

Y

Y

W

W

R

Q

N

A

K

K

G

G

E

E

G

A

Q

-

R

R

R

Q

R

R

F

F

L

L

A

T

F

F

E

R

G

N

S

G

Y
|
Y

H
|
H

G

G

D

D

T

T

F

F

G

G

A

A

M

M

A

S

A

V

-

C

-

D

-

P

-

D

-

N

G

S

V

M

G

H

S

S

G

L

F

W

Y

K

A

G

P

Y

F

L

Q

P

E

E

L

N

L

L

F

F

A

A

V

P

D

A

R

P

M

Q

P

S

Y

R

P

M

A

G

T

E

W

W

D

D

G

E

D

R

P

D

E

M

V

V

E

G

A

-

K

-

E

-

A

-

L

-

D

-

W

-

L

F

D

A

R

R

W

L

L

M

A

A

T

A

N

H

G

R

A

H

E

E

L

I

V

A

A

A

V

V

I

I

E
|
E

P

P

L

I

V

V

Q

Q

G

G

A

A

S

G

G

G

M

M

R

R

F

M

C

Y

R

H

P

P

E

E

F

W

L

L

R

K

A

R

M

I

A

R

A

K

R

I

V

C

R

D

A

R

A

E

G

G

G

I

L

L

V

L

I

I

F

A

D
|
D

E

E

V
x
I

M
x
A

T

T

G

G

F

F

G

G

R

R

T

T

G

G

A

K

L

L

F

F

A

A

S

C

Q

E

K

H

A

A

G

E

V

I

A

A

P

P

D

D

L

I

I

L

C

C

L

L

S

G

K
|
K

G

A

L

L

T

T

G

G

F

T

L

M

P

T

L

L

S

S

V

A

T

T

A

L

C

T

G

T

A

R

S

E

I

V

Y

A

E

E

A

T

F

-

L

I

G

S

A

N

G

G

F

E

D

A

R

G

A

C

F

F

A

M

H

H

G

G

H
x
P

S
x
T

F

F

T

M

A

G

N

N

P

P

L

L

G

A

C

C

A

A

L

N

A

A

S

S

L

L

E

A

L

I

T

L

T

E

S

S

A

G

E

D

T

W

T

Q

A

Q

N

Q

L

V

A

A

R

D

I

I

E

E

T

V

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Q

H

L

R

R

A

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A

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I

L

A

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D

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L

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D

H

A

P

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K

M

L

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S

A

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D

G

V

R

R

V

V

M

L

G

G

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A

I

I

A

G

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I

V

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E

V

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T

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D

H

A

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Y

M

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A

A

A

A

-

V

-

G

-

Q

-

T

-

L

L

K

Q

R

K

F

F

L

V

E

E

R

Q

G

G

L

V

L

W

L

I

R
|
R

P

P

L
x
F

G

G

P

K

V

L

I

I

Y
|
Y

L

L

L

M

P

P

Y

Y

C

I

V

I

T

L

D

P

G

Q

Q

Q

L

L

D

Q

R

R

A

L

Y

T

A

A

I

V

R

N

D

R

A

A

active site: Y17 (≠ F16), Y144 (= Y144), E210 (= E214), D244 (= D248), A247 (≠ M251), K273 (= K277), Y397 (= Y407)
binding cis-amiclenomycin: W52 (= W51), W53 (= W52), K273 (= K277), R390 (= R400), F392 (≠ L402)
binding pyridoxal-5'-phosphate: G112 (= G111), S113 (= S112), Y144 (= Y144), H145 (= H145), D244 (= D248), I246 (≠ V250), K273 (= K277), P307 (≠ H312), T308 (≠ S313)

4w1vA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a thiazole inhibitor (see paper)
38% identity, 98% coverage: 5:430/434 of query aligns to 7:420/425 of 4w1vA

query
sites
4w1vA

V

I

S

A

L

V

D

D

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A

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H

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x
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E

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A

A

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V

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A

G

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A

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L

F

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-

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D

G

G

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-

P

-

I

E

E

I

V

L

L

D

D

L

A

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S

W
|
W

W

W

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T

N

A

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G

H

H

A

G

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H

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P

A

A

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A

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G

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A

A

I

L

A

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Q

A

L

H

R

R

V

L

M

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N

Q

H

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V

I
x
M

F

F

A
x
G

D

G

F

L

T

T

H

H

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E

P

P

A

A

R

R

L

L

A

A

A

K

R

L

L

L

A

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E

D

V

I

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P

P

G

A

G

G

L

L

G

D

R

T

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V

F

F

Y

F

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D

D

N

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G
|
G

S
|
S

T

V

A

S

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E

E

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V

A

A

L

A

K

K

L

M

A

A

W

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Y

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W

R

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N

G

K

R

G

G

E

L

G

P

Q

G

R

K

R

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F

L

L

M

A

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E

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G

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G

Y
|
Y

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|
H

G

G

D

D

T

T

F

F

G

L

A

A

M

M

A

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-

I

A

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G

G

G

F

M

Y

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L

L

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A

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A

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-

A

-

L

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D

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G

A

A

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L

L

V

A

A

A

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|
E

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P

L

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V

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Q

G

G

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G

G

G

M

M

R

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P

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L

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A

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A

R

A

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L

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|
D

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x
I

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x
A

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F

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G

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A

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L

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F

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G

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V

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P

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|
K

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A

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Y

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L

L

S

A

V

A

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C

C

G

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A

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L

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S

A

A

G

G

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G

A

A

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L

A

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H

H

G
|
G

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x
P

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x
T

F

F

T

M

A

A

N

N

P

P

L

L

G

A

C

C

A

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A

V

A

S

L

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A

A

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S

L

V

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L

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D

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P

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A

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S

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V

M

C

G

G

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A

I

I

A

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C

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D

D

R

A

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Q

V

G

D

Y

L

T

A

A

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A

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V

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G

P

Q

A

T

A

L

-

K

-

R

-

F

-

L

L

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D

R

R

G

G

L

V

L

W

L

L

R

R

P

P

L
x
F

G

R

P

N

V

L

I

V

Y
|
Y

L

A

L

M

P

P

Y

Y

C

I

V

C

T

T

D

P

G

A

Q

E

L

I

D

T

R

Q

A

I

Y

T

A

S

A

A

I

M

R

V

D

E

A

V

A

A

active site: Y18 (≠ F16), Y147 (= Y144), E210 (= E214), D244 (= D248), A247 (≠ M251), K273 (= K277), Y397 (= Y407)
binding dimethyl (2R)-5-(3-fluorophenyl)-1H-pyrrolo[1,2-c][1,3]thiazole-6,7-dicarboxylate 2-oxide: P17 (= P15), Y18 (≠ F16), W54 (= W51), M81 (≠ I78), G83 (≠ A80), Y147 (= Y144), G306 (= G311), P307 (≠ H312), T308 (≠ S313), F392 (≠ L402)
binding pyridoxal-5'-phosphate: G114 (= G111), S115 (= S112), Y147 (= Y144), H148 (= H145), E210 (= E214), D244 (= D248), I246 (≠ V250), K273 (= K277)

4cxrA Mycobaterium tuberculosis transaminase bioa complexed with 1-(1,3- benzothiazol-2-yl)methanamine (see paper)
38% identity, 98% coverage: 5:430/434 of query aligns to 7:420/425 of 4cxrA

query
sites
4cxrA

V

I

S

A

L

V

D

D

Q

G

R

A

H

H

V

L

W

W

H

H

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P

F
x
Y

T

S

Q

S

A

I

Q

G

T

R

A

S

P

P

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V

P

V

L

A

A

V

V

A

T

A

H

H

G

G

K

A

G

W

A

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L

L

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I

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-

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D

D

G

G

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-

I

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E

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L

L

D

D

L

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S

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W
|
W

V

T

N

A

L

I

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H

G

G

H

H

A

G

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A

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A

A

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V

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A

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L

L

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L

L

L

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D

V

I

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P

G

A

G

G

L

L

G

D

R

T

V

V

F

F

Y

F

S

S

D

D

N

S

G
|
G

S
|
S

T

V

A

S

V

V

E

E

V

V

A

A

L

A

K

K

L

M

A

A

W

L

Q

Q

Y

Y

W

W

R

R

N

G

K

R

G

G

E

L

G

P

Q

G

R

K

R

R

F

L

L

M

A

T

F

W

E

R

G

G

S

G

Y
|
Y

H
|
H

G

G

D

D

T

T

F

F

G

L

A

A

M

M

A

S

-

I

A

C

G

D

V

P

G

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S

G

G

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M

Y

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L

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W

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T

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D

L

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L

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A

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M

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D

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Y

D

D

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P

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A

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Y

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A

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-

A

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L

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W

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D

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L

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A

A

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L

L

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A

A

A

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V

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|
E

P

P

L

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V

V

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Q

G

G

A
|
A

S

G

G

G

M

M

R

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F

F

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P

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G

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L

L

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L

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|
D

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I

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x
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G

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F

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R

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L

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|
K

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Y

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L

L

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A

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G

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G

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x
P

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x
T

F

F

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A

A

N

N

P

P

L

L

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G

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D

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Y

L

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A

A

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P

Q

A

T

A

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-

K

-

R

-

F

-

L

L

E

D

R

R

G

G

L

V

L

W

L

L

R

R

P

P

L

F

G

R

P

N

V

L

I

V

Y
|
Y

L

A

L

M

P

P

Y

Y

C

I

V

C

T

T

D

P

G

A

Q

E

L

I

D

T

R

Q

A

I

Y

T

A

S

A

A

I

M

R

V

D

E

A

V

A

A

active site: Y18 (≠ F16), Y147 (= Y144), E210 (= E214), D244 (= D248), A247 (≠ M251), K273 (= K277), Y397 (= Y407)
binding 1-(1,3-benzothiazol-2-yl)methanamine: Y18 (≠ F16), W54 (= W51), W55 (= W52), A216 (= A220)
binding pyridoxal-5'-phosphate: G114 (= G111), S115 (= S112), Y147 (= Y144), H148 (= H145), E210 (= E214), D244 (= D248), I246 (≠ V250), K273 (= K277), P307 (≠ H312), T308 (≠ S313)

4cxqA Mycobaterium tuberculosis transaminase bioa complexed with substrate kapa (see paper)
38% identity, 98% coverage: 5:430/434 of query aligns to 7:422/427 of 4cxqA

query
sites
4cxqA

V

I

S

A

L

V

D

D

Q

G

R

A

H

H

V

L

W

W

H

H

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x
Y

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A

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V

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A

V

V

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H

G

G

A

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W

L

L

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L

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D

D

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G

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P

-

I

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V

L

L

D

D

L

A

I

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S

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W
|
W

W

W

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T

N

A

L

I

H

H

G

G

H

H

A

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A

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F

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A

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L

L

A

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R

L

L

L

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P

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A

G

G

L

L

G

D

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T

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V

F

F

Y

F

S

S

D

D

N

S

G
|
G

S
|
S

T

V

A

S

V

V

E

E

V

V

A

A

L

A

K

K

L

M

A

A

W

L

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Q

Y

Y

W

W

R

R

N

G

K

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G

G

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L

G

P

Q

G

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K

R

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F

L

L

M

A

T

F

W

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R

G

G

S

G

Y
|
Y

H
|
H

G
|
G

D

D

T

T

F

F

G

L

A

A

M

M

A

S

-

I

A

C

G

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D

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A

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-

A

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A

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L

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A

A

A

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|
E

P

P

L

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V

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Q

G

G

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G

G

G

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M

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L

L

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L

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D
|
D

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x
I

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x
A

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G

G

F

F

G

G

R

R

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A

L

L

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F

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A

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A

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D

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active site: Y18 (≠ F16), Y149 (= Y144), E212 (= E214), D246 (= D248), A249 (≠ M251), K275 (= K277), Y399 (= Y407)
binding 7-keto-8-aminopelargonic acid: W56 (= W51), Y149 (= Y144), G308 (= G311), T310 (≠ S313), R392 (= R400)
binding pyridoxal-5'-phosphate: G116 (= G111), S117 (= S112), Y149 (= Y144), H150 (= H145), G151 (= G146), E212 (= E214), D246 (= D248), I248 (≠ V250), K275 (= K277), P309 (≠ H312), T310 (≠ S313)

5kgtA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with an inhibitor optimized from hts lead: 1-[4-[4-(3-chlorophenyl)carbonylpiperidin-1- yl]phenyl]ethanone (see paper)
38% identity, 98% coverage: 5:430/434 of query aligns to 7:423/429 of 5kgtA

query
sites
5kgtA

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active site: Y18 (≠ F16), Y150 (= Y144), E213 (= E214), D247 (= D248), A250 (≠ M251), K276 (= K277), Y400 (= Y407)
binding 1-[4-[4-(3-chlorophenyl)carbonylpiperidin-1-yl]phenyl]ethanone: M84 (≠ I78), G86 (≠ A80), G309 (= G311), T311 (≠ S313)
binding pyridoxal-5'-phosphate: S116 (≠ N110), G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), G152 (= G146), E213 (= E214), D247 (= D248), I249 (≠ V250), K276 (= K277)

5kgsA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with an inhibitor optimized from hts lead: 5-[4-(1,3-benzodioxol-5-ylcarbonyl)piperazin-1-yl]-2, 3-dihydroinden-1-one (see paper)
38% identity, 98% coverage: 5:430/434 of query aligns to 7:423/429 of 5kgsA

query
sites
5kgsA

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active site: Y18 (≠ F16), Y150 (= Y144), E213 (= E214), D247 (= D248), A250 (≠ M251), K276 (= K277), Y400 (= Y407)
binding 5-[4-(1,3-benzodioxol-5-ylcarbonyl)piperazin-1-yl]-2,3-dihydroinden-1-one: P17 (= P15), Y18 (≠ F16), W57 (= W51), M84 (≠ I78), G86 (≠ A80), Y150 (= Y144), D162 (≠ G155), G165 (≠ S158), G166 (= G159), P310 (≠ H312), T311 (≠ S313), F395 (≠ L402)
binding pyridoxal-5'-phosphate: G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), G152 (= G146), E213 (= E214), D247 (= D248), I249 (≠ V250), K276 (= K277)

4xjpA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with an inhibitor optimized from hts lead (see paper)
38% identity, 98% coverage: 5:430/434 of query aligns to 7:423/429 of 4xjpA

query
sites
4xjpA

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T

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W

R

D

D

G

Y

D

D

P

P

E

A

V

Y

E

S

A

A

K

A

E

F

A

E

A

A

A

Q

L

-

D

-

W

-

L

-

D

-

R

-

W

-

L

L

A

A

T

Q

N

H

G

A

A

G

E

E

L

L

V

A

A

A

V

V

I

V

E
|
E

P

P

L

V

V

V

Q

Q

G

G

A
|
A

S
x
G

G

G

M

M

R

R

F

F

C

H

R

D

P

P

E

R

F

Y

L

L

R

H

A

D

M

L

A

R

A

D

R

I

V

C

R

R

A

R

A

Y

G

E

G

V

L

L

V

L

I

I

F

F

D
|
D

E

E

V
x
I

M
x
A

T

T

G

G

F

F

G

G

R

R

T

T

G

G

A

A

L

L

F

F

A

A

S

A

Q

D

K

H

A

A

G

G

V

V

A

S

P

P

D

D

L

I

I

M

C

C

L

V

S

G

K
|
K

G

A

L

L

T

T

G

G

F

Y

L

L

P

S

L

L

S

A

V

A

T

T

A

L

C

C

G

T

A

A

S

D

I

V

Y

A

E

H

A

T

F

-

L

I

G

S

A

A

G

G

F

A

D

A

R

G

A

A

F

L

A

M

H

H

G
|
G

H
x
P

S
x
T

F

F

T

M

A

A

N

N

P

P

L

L

G

A

C

C

A

A

A

V

A

S

L

V

A

A

S

S

L

V

E

E

L

L

T

L

S

G

A

Q

E

D

T

W

T

R

A

T

N

R

L

I

A

T

R

E

I

L

E

A

T

A

R

G

H

L

R

T

A

A

A

G

I

L

A

D

D

T

L

A

S

R

G

A

H

L

P

P

K

A

L

V

S

T

R

D

G

V

R

R

V

V

M

C

G

G

T

A

I

I

A

G

A

V

I

I

E

E

V

C

T

D

D

R

A

P

Q

V

G

D

Y

L

T

A

A

V

A

A

V

T

G

P

Q

A

T

A

L

-

K

-

R

-

F

-

L

L

E

D

R

R

G

G

L

V

L

W

L

L

R

R

P

P

L
x
F

G

R

P

N

V

L

I

V

Y
|
Y

L

A

L

M

P

P

Y

Y

C

I

V

C

T

T

D

P

G

A

Q

E

L

I

D

T

R

Q

A

I

Y

T

A

S

A

A

I

M

R

V

D

E

A

V

A

A

active site: Y18 (≠ F16), Y150 (= Y144), E213 (= E214), D247 (= D248), A250 (≠ M251), K276 (= K277), Y400 (= Y407)
binding 1-{4-[4-(1,3-benzodioxol-5-ylcarbonyl)piperazin-1-yl]phenyl}ethanone: P17 (= P15), Y18 (≠ F16), W57 (= W51), M84 (≠ I78), G86 (≠ A80), Y150 (= Y144), G165 (≠ S158), G166 (= G159), A219 (= A220), G220 (≠ S221), G309 (= G311), F395 (≠ L402)
binding pyridoxal-5'-phosphate: G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), G152 (= G146), E213 (= E214), D247 (= D248), I249 (≠ V250), K276 (= K277), P310 (≠ H312), T311 (≠ S313)

4xjmA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a hts lead compound
38% identity, 98% coverage: 5:430/434 of query aligns to 7:423/429 of 4xjmA

query
sites
4xjmA

V

I

S

A

L

V

D

D

Q

G

R

A

H

H

V

L

W

W

H

H

P
|
P

F
x
Y

T

S

-

S

-

I

-

G

Q

R

A

E

Q

A

T

V

A

S

P

P

E

V

P

V

L

A

A

V

V

A

T

A

H

H

G

G

K

A

G

W

A

L

S

T

L

L

F

I

T

-

E

R

D

D

G

G

R

Q

-

P

-

I

E

E

I

V

L

L

D

D

L

A

I

M

S

S

W
|
W

W

W

V

T

N

A

L

I

H

H

G

G

H

H

A

G

H

H

P

P

A

A

V

L

A

D

G

Q

A

A

I

L

A

T

E

T

Q

Q

A

L

H

R

R

V

L

M

E

N

Q

H

V

V

I
x
M

F

F

A
x
G

D

G

F

L

T

T

H

H

S

E

P

P

A

A

R

R

L

L

A

A

A

K

R

L

L

L

A

V

E

D

V

I

L

T

P

P

G

A

G

G

L

L

G

D

R

T

V

V

F

F

Y

F

S

S

D

D

N

S

G
|
G

S
|
S

T

V

A

S

V

V

E

E

V

V

A

A

L

A

K

K

L

M

A

A

W

L

Q

Q

Y

Y

W

W

R

R

N

G

K

R

G

G

E

L

G

P

Q

G

R

K

R

R

F

L

L

M

A

T

F

W

E

R

G

G

S

G

Y
|
Y

H
|
H

G
|
G

D

D

T

T

F

F

G

L

A

A

M
|
M

A

S

-

I

A

C

G

D

V

P

G

H

S
x
G

G
|
G

F
x
M

Y

H

A

S

P

L

F
x
W

Q

T

E

D

L

V

L

L

F

A

A

A

V

Q

D

V

R

F

M

A

P

P

Y

Q

P

-

A

V

T

P

W

R

D

D

G

Y

D

D

P

P

E

A

V

Y

E

S

A

A

K

A

E

F

A

E

A

A

A

Q

L

-

D

-

W

-

L

-

D

-

R

-

W

-

L

L

A

A

T

Q

N

H

G

A

A

G

E

E

L

L

V

A

A

A

V

V

I

V

E
|
E

P

P

L

V

V

V

Q

Q

G

G

A

A

S

G

G

G

M

M

R

R

F

F

C

H

R

D

P

P

E

R

F

Y

L

L

R

H

A

D

M

L

A

R

A

D

R

I

V

C

R

R

A

R

A

Y

G

E

G

V

L

L

V

L

I

I

F

F

D
|
D

E

E

V
x
I

M
x
A

T

T

G

G

F

F

G

G

R

R

T

T

G

G

A

A

L

L

F

F

A

A

S

A

Q

D

K

H

A

A

G

G

V

V

A

S

P

P

D

D

L

I

I

M

C

C

L

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G

K
|
K

G

A

L

L

T

T

G

G

F

Y

L

L

P

S

L

L

S

A

V

A

T

T

A

L

C

C

G

T

A

A

S

D

I

V

Y

A

E

H

A

T

F

-

L

I

G

S

A

A

G

G

F

A

D

A

R

G

A

A

F

L

A
x
M

H

H

G
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G

H
x
P

S
x
T

F

F

T

M

A

A

N

N

P

P

L

L

G

A

C

C

A

A

A

V

A

S

L

V

A

A

S

S

L

V

E

E

L

L

T

L

S

G

A

Q

E

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T

W

T

R

A

T

N

R

L

I

A

T

R

E

I

L

E

A

T

A

R

G

H

L

R

T

A

A

A

G

I

L

A

D

D

T

L

A

S

R

G

A

H

L

P

P

K

A

L

V

S

T

R

D

G

V

R

R

V

V

M

C

G

G

T

A

I

I

A

G

A

V

I

I

E

E

V

C

T

D

D

R

A

P

Q

V

G

D

Y

L

T

A

A

V

A

A

V

T

G

P

Q

A

T

A

L

-

K

-

R

-

F

-

L

L

E

D

R

R

G

G

L

V

L

W

L

L

R

R

P

P

L

F

G

R

P

N

V

L

I

V

Y
|
Y

L

A

L

M

P

P

Y

Y

C

I

V

C

T

T

D

P

G

A

Q

E

L

I

D

T

R

Q

A

I

Y

T

A

S

A

A

I

M

R

V

D

E

A

V

A

A

active site: Y18 (≠ F16), Y150 (= Y144), E213 (= E214), D247 (= D248), A250 (≠ M251), K276 (= K277), Y400 (= Y407)
binding 3-{1-[(5-acetylthiophen-2-yl)carbonyl]piperidin-4-yl}-N-(3-methoxyphenyl)propanamide: P17 (= P15), Y18 (≠ F16), W57 (= W51), M84 (≠ I78), G86 (≠ A80), Y150 (= Y144), M158 (= M152), G165 (≠ S158), G166 (= G159), M167 (≠ F160), W171 (≠ F164), M307 (≠ A309), G309 (= G311), T311 (≠ S313)
binding pyridoxal-5'-phosphate: G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), G152 (= G146), E213 (= E214), D247 (= D248), I249 (≠ V250), K276 (= K277), P310 (≠ H312), T311 (≠ S313)

4xjlA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a hts lead compound
38% identity, 98% coverage: 5:430/434 of query aligns to 7:423/429 of 4xjlA

query
sites
4xjlA

V

I

S

A

L

V

D

D

Q

G

R

A

H

H

V

L

W

W

H

H

P
|
P

F
x
Y

T

S

-

S

-

I

-

G

Q

R

A

E

Q

A

T

V

A

S

P

P

E

V

P

V

L

A

A

V

V

A

T

A

H

H

G

G

K

A

G

W

A

L

S

T

L

L

F

I

T

-

E

R

D

D

G

G

R

Q

-

P

-

I

E

E

I

V

L

L

D

D

L

A

I

M

S

S

W
|
W

W

W

V

T

N

A

L

I

H

H

G

G

H

H

A

G

H

H

P

P

A

A

V

L

A

D

G

Q

A

A

I

L

A

T

E

T

Q

Q

A

L

H

R

R

V

L

M

E

N

Q

H

V

V

I
x
M

F

F

A
x
G

D

G

F

L

T

T

H

H

S

E

P

P

A

A

R

R

L

L

A

A

A

K

R

L

L

L

A

V

E

D

V

I

L

T

P

P

G

A

G

G

L

L

G

D

R

T

V

V

F

F

Y

F

S

S

D

D

N

S

G
|
G

S
|
S

T

V

A

S

V

V

E

E

V

V

A

A

L

A

K

K

L

M

A

A

W

L

Q

Q

Y

Y

W

W

R

R

N

G

K

R

G

G

E

L

G

P

Q

G

R

K

R

R

F

L

L

M

A

T

F

W

E

R

G

G

S

G

Y
|
Y

H
|
H

G
|
G

D

D

T

T

F

F

G

L

A

A

M

M

A

S

-

I

A
x
C

G

D

V

P

G

H

S
x
G

G
|
G

F

M

Y

H

A

S

P

L

F

W

Q

T

E

D

L

V

L

L

F

A

A

A

V

Q

D

V

R

F

M

A

P

P

Y

Q

P

-

A

V

T

P

W

R

D

D

G

Y

D

D

P

P

E

A

V

Y

E

S

A

A

K

A

E

F

A

E

A

A

A

Q

L

-

D

-

W

-

L

-

D

-

R

-

W

-

L

L

A

A

T

Q

N

H

G

A

A

G

E

E

L

L

V

A

A

A

V

V

I

V

E
|
E

P

P

L

V

V

V

Q

Q

G

G

A
|
A

S

G

G

G

M

M

R

R

F

F

C

H

R

D

P

P

E

R

F

Y

L

L

R

H

A

D

M

L

A

R

A

D

R

I

V

C

R

R

A

R

A

Y

G

E

G

V

L

L

V

L

I

I

F

F

D
|
D

E

E

V
x
I

M
x
A

T

T

G

G

F

F

G

G

R

R

T

T

G

G

A

A

L

L

F

F

A

A

S

A

Q

D

K

H

A

A

G

G

V

V

A

S

P

P

D

D

L

I

I

M

C

C

L

V

S

G

K
|
K

G

A

L

L

T

T

G

G

F

Y

L

L

P

S

L

L

S

A

V

A

T

T

A

L

C

C

G

T

A

A

S

D

I

V

Y

A

E

H

A

T

F

-

L

I

G

S

A

A

G

G

F

A

D

A

R

G

A

A

F

L

A

M

H

H

G

G

H
x
P

S
x
T

F

F

T

M

A

A

N

N

P

P

L

L

G

A

C

C

A

A

A

V

A

S

L

V

A

A

S

S

L

V

E

E

L

L

T

L

S

G

A

Q

E

D

T

W

T

R

A

T

N

R

L

I

A

T

R

E

I

L

E

A

T

A

R

G

H

L

R

T

A

A

A

G

I

L

A

D

D

T

L

A

S

R

G

A

H

L

P

P

K

A

L

V

S

T

R

D

G

V

R

R

V

V

M

C

G

G

T

A

I

I

A

G

A

V

I

I

E

E

V

C

T

D

D

R

A

P

Q

V

G

D

Y

L

T

A

A

V

A

A

V

T

G

P

Q

A

T

A

L

-

K

-

R

-

F

-

L

L

E

D

R

R

G

G

L

V

L

W

L

L

R

R

P

P

L

F

G

R

P

N

V

L

I

V

Y
|
Y

L

A

L

M

P

P

Y

Y

C

I

V

C

T

T

D

P

G

A

Q

E

L

I

D

T

R

Q

A

I

Y

T

A

S

A

A

I

M

R

V

D

E

A

V

A

A

active site: Y18 (≠ F16), Y150 (= Y144), E213 (= E214), D247 (= D248), A250 (≠ M251), K276 (= K277), Y400 (= Y407)
binding N-(1,2,3-benzothiadiazol-5-yl)-4-phenylpiperazine-1-carboxamide: P17 (= P15), Y18 (≠ F16), W57 (= W51), M84 (≠ I78), G86 (≠ A80), Y150 (= Y144), C161 (≠ A154), G165 (≠ S158), G166 (= G159), A219 (= A220)
binding pyridoxal-5'-phosphate: G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), G152 (= G146), E213 (= E214), D247 (= D248), I249 (≠ V250), K276 (= K277), P310 (≠ H312), T311 (≠ S313)

4wygA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis complexed with a fragment hit (see paper)
38% identity, 98% coverage: 5:430/434 of query aligns to 7:423/429 of 4wygA

query
sites
4wygA

V

I

S

A

L

V

D

D

Q

G

R

A

H

H

V

L

W

W

H

H

P

P

F
x
Y

T

S

-

S

-

I

-

G

Q

R

A

E

Q

A

T

V

A

S

P

P

E

V

P

V

L

A

A

V

V

A

T

A

H

H

G

G

K

A

G

W

A

L

S

T

L

L

F

I

T

-

E

R

D

D

G

G

R

Q

-

P

-

I

E

E

I

V

L

L

D

D

L

A

I

M

S

S

W
|
W

V

T

N

A

L

I

H

H

G

G

H

H

A

G

H

H

P

P

A

A

V

L

A

D

G

Q

A

A

I

L

A

T

E

T

Q

Q

A

L

H

R

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active site: Y18 (≠ F16), Y150 (= Y144), E213 (= E214), D247 (= D248), A250 (≠ M251), K276 (= K277), Y400 (= Y407)
binding 1-{4-[(4-chloro-1H-pyrazol-1-yl)methyl]phenyl}methanamine: Y18 (≠ F16), W57 (= W51), W58 (= W52), Y150 (= Y144), A219 (= A220), F395 (≠ L402)
binding pyridoxal-5'-phosphate: G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), E213 (= E214), D247 (= D248), I249 (≠ V250), K276 (= K277), P310 (≠ H312), T311 (≠ S313)

4wyeA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis complexed with a dsf fragment hit (see paper)
38% identity, 98% coverage: 5:430/434 of query aligns to 7:423/429 of 4wyeA

query
sites
4wyeA

V

I

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A

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A

active site: Y18 (≠ F16), Y150 (= Y144), E213 (= E214), D247 (= D248), A250 (≠ M251), K276 (= K277), Y400 (= Y407)
binding phenyl(piperidin-4-yl)methanone: Y18 (≠ F16), W57 (= W51), W58 (= W52), A219 (= A220), F395 (≠ L402), Y400 (= Y407)
binding pyridoxal-5'-phosphate: G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), G152 (= G146), E213 (= E214), D247 (= D248), I249 (≠ V250), K276 (= K277), P310 (≠ H312), T311 (≠ S313)

4w1xA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with 1-(4-(4-(3-chlorobenzoyl) piperazin-1-yl)phenyl)ethanone (see paper)
38% identity, 98% coverage: 5:430/434 of query aligns to 7:423/429 of 4w1xA

query
sites
4w1xA

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A

active site: Y18 (≠ F16), Y150 (= Y144), E213 (= E214), D247 (= D248), A250 (≠ M251), K276 (= K277), Y400 (= Y407)
binding 1-{4-[4-(3-chlorobenzoyl)piperazin-1-yl]phenyl}ethanone: P17 (= P15), Y18 (≠ F16), W57 (= W51), M84 (≠ I78), G86 (≠ A80), Y150 (= Y144), G165 (≠ S158), G166 (= G159), A219 (= A220), G309 (= G311), T311 (≠ S313)
binding pyridoxal-5'-phosphate: G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), G152 (= G146), E213 (= E214), D247 (= D248), I249 (≠ V250), K276 (= K277), P310 (≠ H312), T311 (≠ S313)

4w1wA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with 7-(diethylamino)-3- (thiophene-2-carbonyl)-2h-chromen-2-one (see paper)
38% identity, 98% coverage: 5:430/434 of query aligns to 7:423/429 of 4w1wA

query
sites
4w1wA

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active site: Y18 (≠ F16), Y150 (= Y144), E213 (= E214), D247 (= D248), A250 (≠ M251), K276 (= K277), Y400 (= Y407)
binding 7-(diethylamino)-3-(thiophen-2-ylcarbonyl)-2H-chromen-2-one: P17 (= P15), Y18 (≠ F16), W57 (= W51), M84 (≠ I78), G86 (≠ A80), Y150 (= Y144), G165 (≠ S158), G309 (= G311), P310 (≠ H312), R393 (= R400)
binding pyridoxal-5'-phosphate: G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), G152 (= G146), E213 (= E214), D247 (= D248), I249 (≠ V250), K276 (= K277), P310 (≠ H312), T311 (≠ S313)

4wydA Adenosylmethionine-8-amino-7-oxononanoate aminotransferase from mycobacterium tuberculosis complexed with a fragment from dsf screening (see paper)
38% identity, 98% coverage: 5:430/434 of query aligns to 7:423/430 of 4wydA

query
sites
4wydA

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active site: Y18 (≠ F16), Y150 (= Y144), E213 (= E214), D247 (= D248), A250 (≠ M251), K276 (= K277), Y400 (= Y407)
binding N-methyl-1-[4-(1H-pyrazol-1-ylmethyl)phenyl]methanamine: Y18 (≠ F16), W57 (= W51), W58 (= W52), Y150 (= Y144), A219 (= A220), G309 (= G311), T311 (≠ S313), F395 (≠ L402)
binding pyridoxal-5'-phosphate: G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), E213 (= E214), D247 (= D248), I249 (≠ V250), K276 (= K277), P310 (≠ H312), T311 (≠ S313)

P9WQ81 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; 7,8-diaminopelargonic acid synthase; DAPAS; Diaminopelargonic acid synthase; EC 2.6.1.62 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
38% identity, 98% coverage: 5:430/434 of query aligns to 14:430/437 of P9WQ81

query
sites
P9WQ81

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N

R

L

I

A

T

R

E

I

L

E

A

T

A

R

G

H

L

R

T

A

A

A

G

I

L

A

D

D

T

L

A

S

R

G

A

H

L

P

P

K

A

L

V

S

T

R

D

G

V

R

R

V

V

M

C

G

G

T

A

I

I

A

G

A

V

I

I

E

E

V

C

T

D

D

R

A

P

Q

V

G

D

Y

L

T

A

A

V

A

A

V

T

G

P

Q

A

T

A

L

-

K

-

R

-

F

-

L

L

E

D

R

R

G

G

L

V

L

W

L

L

R

R

P

P

L

F

G

R

P

N

V

L

I

V

Y

Y

L

A

L

M

P

P

Y

Y

C

I

V

C

T

T

D

P

G

A

Q

E

L

I

D

T

R

Q

A

I

Y

T

A

S

A

A

I

M

R

V

D

E

A

V

A

A

Y25 (≠ F16) mutation to A: Does not show detectable activity at 335 nm with SAM, even up to concentrations of 3 mM, and shows approximately 70% reduced activity with high concentrations of DAPA (0.5 mM).
W64 (= W51) binding
Y157 (= Y144) binding
K283 (= K277) modified: N6-(pyridoxal phosphate)lysine
G316 (= G311) binding

Query Sequence

>AZOBR_RS31120 FitnessBrowser__azobra:AZOBR_RS31120
MTDTVSLDQRHVWHPFTQAQTAPEPLAVTHGKGASLFTEDGREILDLISSWWVNLHGHAH
PAVAGAIAEQAHRLEQVIFADFTHSPAARLAARLAEVLPGGLGRVFYSDNGSTAVEVALK
LAWQYWRNKGEGQRRRFLAFEGSYHGDTFGAMAAGVGSGFYAPFQELLFAVDRMPYPATW
DGDPEVEAKEAAALDWLDRWLATNGAELVAVIIEPLVQGASGMRFCRPEFLRAMAARVRA
AGGLVIFDEVMTGFGRTGALFASQKAGVAPDLICLSKGLTGGFLPLSVTACGASIYEAFL
GAGFDRAFAHGHSFTANPLGCAAALASLELTTSAETTANLARIETRHRAAIADLSGHPKL
SRGRVMGTIAAIEVTDAQGYTAAVGQTLKRFFLERGLLLRPLGPVIYLLPPYCVTDGQLD
RAYAAIRDAADTLL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory