SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AZOBR_RS31230 FitnessBrowser__azobra:AZOBR_RS31230 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9A9Z1 D-xylonolactone lactonase; Xylono-1,5-lactonase; EC 3.1.1.110 from Caulobacter vibrioides (strain ATCC 19089 / CIP 103742 / CB 15) (Caulobacter crescentus) (see paper)
41% identity, 97% coverage: 1:290/299 of query aligns to 1:282/289 of Q9A9Z1

query
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Q9A9Z1

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E17 (= E17) binding Fe(2+)
N145 (= N153) binding Fe(2+)
D195 (= D202) binding Fe(2+)

7pldB Caulobacter crescentus xylonolactonase with (r)-4-hydroxy-2- pyrrolidone (see paper)
41% identity, 96% coverage: 4:290/299 of query aligns to 4:282/289 of 7pldB

query
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binding (R)-4-hydroxy-2-pyrrolidone: L15 (= L15), E17 (= E17), I31 (= I33), A64 (≠ G66), G66 (= G68), F67 (= F69), P80 (≠ L83), R98 (= R105), N100 (= N107), E119 (= E126), D138 (= D146), N145 (= N153), K180 (= K188), D195 (= D202), W210 (= W217), W210 (= W217)
binding fe (ii) ion: E17 (= E17), N145 (= N153), D195 (= D202)

7plbB Caulobacter crescentus xylonolactonase with d-xylose (see paper)
41% identity, 96% coverage: 4:290/299 of query aligns to 4:282/289 of 7plbB

query
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7plbB

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binding fe (ii) ion: E17 (= E17), N145 (= N153), D195 (= D202)
binding beta-D-xylopyranose: L15 (= L15), E17 (= E17), E89 (≠ R96), V90 (≠ I97), D92 (≠ P99), R98 (= R105), N100 (= N107), R109 (= R116), D130 (= D137), N145 (= N153), D174 (≠ A182), D195 (= D202)
binding alpha-D-xylopyranose: A64 (≠ G66), G66 (= G68), F67 (= F69), P80 (≠ L83)

3g4hA Crystal structure of human senescence marker protein-30 (zinc bound) (see paper)
32% identity, 98% coverage: 4:295/299 of query aligns to 3:296/297 of 3g4hA

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binding zinc ion: E16 (= E17), N152 (= N153), D202 (= D202)

Q15493 Regucalcin; RC; Gluconolactonase; GNL; Senescence marker protein 30; SMP-30; EC 3.1.1.17 from Homo sapiens (Human) (see 2 papers)
32% identity, 98% coverage: 4:295/299 of query aligns to 5:298/299 of Q15493

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Q15493

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I

V

L

C

R

R

H

W

G

D

L

-

D

S

D

F

G

T

S

K

Q

Q

A

V

D

Q

W

R

A

V

L

T

E

M

D

D

A

A

A

P

C

V

W

S

L

S

V

V

E

A

C

L

A

R

D

Q

G

S

D

G

G

G

F

Y

I

V

A

A

G

T

L

I

R

G

S

T

R

K

R

-

V

F

V

C

R

A

L

L

R

N

L

W

E

K

P

E

G

Q

R

S

A

A

V

V

I

V

A

-

G

-

E

-

L

L

A

A

R

T

I

V

D

D

P

N

D

D

R

K

P

K

G

N

N

N

R

R

L

F

N
|
N

D

D

A

G

K

K

A

V

D

D

A

P

Q

A

G

G

R

R

L

Y

W

F

I

A

G

G

S

T

M

M

D

-

G

A

E

E

T

T

P

A

-

P

-

A

-

V

-

L

-

E

-

R

-

H

S

Q

G

G

A

A

F

L

H

Y

R

S

L

L

D

F

P

P

D

D

G

H

S

H

I

V

T

K

R

K

M

Y

D

F

E

D

G

Q

Y

V

T

D

V

I

A

S

N
|
N

G

G

P

L

A

D

L

W

S

S

P

L

D

D

G

H

R

K

T

I

L

F

Y

Y

H

Y

T

I

D

D

S

S

A

L

A

S

R

Y

T

S

I

V

H

D

A

A

F

F

D

D

L

Y

D

D

-

L

G

Q

A

T

G

G

R

Q

L

I

S

S

G

N

K

R

R

R

A

S

H

V

I

Y

R

K

F

L

A

E

E

K

A

E

D

E

G

Q

Y

I

P

P

D
|
D

G

G

M

M

T

C

C

I

D

D

A

A

E

E

G

G

G

K

L

L

W

W

V

V

A

A

H

C

W

Y

D

N

G

G

R

R

V

V

S

I

R

R

F

L

R

D

P

P

-

V

D

T

G

G

T

K

L

R

D

L

R

Q

A

T

I

V

A

K

L

L

P

P

V

V

S

D

R

K

V

T

T

T

S

S

C

C

V

C

F

F

A

G

G

G

P

K

A

N

L

Y

D

S

R

E

L

M

F

Y

V

V

T

T

T

C

A

A

A

R

H

D

G

G

I

M

G

D

R

P

D

E

G

G

R

L

P

L

D

R

E

Q

P

P

L

E

A

A

G

G

A

G

L

I

F

F

E

K

C

I

D

T

P

G

G

L

G

G

V

V

R

K

G

G

L

I

P

A

P

P

G

Y

R

S

F

Y

G

A

E18 (= E17) binding a divalent metal cation; mutation to A: Reduces enzyme activity by about 90%.
N103 (= N107) mutation to A: Reduces enzyme activity by about 95%.
N154 (= N153) binding a divalent metal cation; mutation to A: Reduces enzyme activity by about 95%.
D204 (= D202) binding a divalent metal cation; mutation to A: Reduces enzyme activity by over 98%.

4gncA Human smp30/gnl-1,5-ag complex (see paper)
32% identity, 98% coverage: 4:295/299 of query aligns to 4:297/298 of 4gncA

query
sites
4gncA

E

K

V

I

R

E

C

C

V

V

W

L

P

P

A

E

R

N

A

C

L

R

L

C

G

G

E
|
E

G

S

P

P

L

V

W

W

S

E

P

E

E

V

Q

S

G

N

A

S

V

L

F

L

F

F

V

V

D

D

I

I

H

P

G

A

S

K

R

K

I

V

L

C

R

R

H

W

G

D

L

-

D

S

D

F

G

T

S

K

Q

Q

A

V

D

Q

W

R

A

V

L

T

E

M

D

D

A

A

A

P

C

V

W

S

L

S

V

V

E

A

C

L

A

R

D

Q

G

S

D

G

G

G

F

Y

I

V

A

A

G

T

L

I

R

G

S

T

R

K

R

-

V

F

V

C

R

A

L

L

R

N

L

W

E

K

P

E

G

Q

R

S

A

A

V

V

I

V

A

-

G

-

E

-

L

L

A

A

R

T

I

V

D

D

P

N

D

D

R

K

P

K

G

N

N

N

R
|
R

L

F

N
|
N

D
|
D

A

G

K

K

A

V

D

D

A

P

Q

A

G

G

R

R

L

Y

W

F

I

A

G

G

S

T

M

M

D

-

G

A

E

E

T

T

P

A

-

P

-

A

-

V

-

L

-

E

-

R

-

H

S

Q

G

G

A

A

F

L

H

Y

R

S

L

L

D

F

P

P

D

D

G

H

S

H

I

V

T

K

R

K

M

Y

D

F

E

D

G

Q

Y

V

T

D

V

I

A

S

N
|
N

G

G

P

L

A

D

L

W

S

S

P

L

D

D

G

H

R

K

T

I

L

F

Y

Y

H

Y

T

I

D

D

S

S

A

L

A

S

R

Y

T

S

I

V

H

D

A

A

F

F

D

D

L

Y

D

D

-

L

G

Q

A

T

G

G

R

Q

L

I

S

S

G

N

K

R

R

R

A

S

H

V

I

Y

R

K

F

L

A

E

E

K

A

E

D

E

G

Q

Y

I

P

P

D
|
D

G

G

M

M

T

C

C

I

D

D

A

A

E

E

G

G

G

K

L

L

W

W

V

V

A

A

H

C

W

Y

D

N

G

G

R

R

V

V

S

I

R

R

F

L

R

D

P

P

-

V

D

T

G

G

T

K

L

R

D

L

R

Q

A

T

I

V

A

K

L

L

P

P

V

V

S

D

R

K

V

T

T
|
T

S

S

C

C

V

C

F

F

A

G

G

G

P

K

A
x
N

L
x
Y

D

S

R

E

L

M

F

Y

V

V

T

T

T

C

A

A

A

R

H

D

G

G

I

M

G

D

R

P

D

E

G

G

R

L

P

L

D

R

E

Q

P

P

L

E

A

A

G

G

A

G

L

I

F

F

E

K

C

I

D

T

P

G

G

L

G

G

V

V

R

K

G
|
G

L
x
I

P

A

P

P

G

Y

R

S

F

Y

G

A

binding 1,5-anhydro-D-glucitol: E17 (= E17), R100 (= R105), N102 (= N107), D203 (= D202), T245 (= T243), N253 (≠ A251), Y254 (≠ L252), G290 (= G288), I291 (≠ L289)
binding calcium ion: E17 (= E17), D103 (= D108), N153 (= N153), D203 (= D202)

4gnaA Mouse smp30/gnl-xylitol complex (see paper)
33% identity, 98% coverage: 4:295/299 of query aligns to 3:296/297 of 4gnaA

query
sites
4gnaA

E

K

V

V

R

E

C

C

V

V

W

L

P

R

A

E

R

N

A

Y

L

R

L

C

G

G

E
|
E

G

S

P

P

L

V

W

W

S

E

P

E

E

A

Q

S

G

Q

A

S

V

L

F

L

F

F

V

V

D

D

I

I

H

P

G

-

S

S

R

K

I

I

L

I

R

C

H

R

G

W

L

D

D

T

D

V

G

S

S

N

Q

Q

A

V

D

Q

W

R

A

V

L

A

E

V

D

D

A

A

A

P

C

V

W

S

L

S

V

V

E

A

C

L

A

R

D

Q

G

L

D

G

G

G

F

Y

I

V

A

A

G

T

L

I

R

G

S

T

R

K

R

-

V

F

V

C

R

A

L

L

R

N

L

W

E

E

P

N

G

Q

R

S

A

V

V

F

I

V

A

-

G

-

E

-

L

L

A

A

R

M

I

V

D

D

P

E

D

D

R

K

P

K

G

N

N

N

R
|
R

L

F

N
|
N

D

D

A

G

K

K

A

V

D

D

A

P

Q

A

G

G

R

R

L

Y

W

F

I

A

G

G

S

T

M

M

D

-

G

A

E

E

E
|
E

T

T

P

A

-

P

-

A

-

V

-

L

-

E

-

R

-

H

S

Q

G

G

A

S

F

L

H

Y

R

S

L

L

D

F

P

P

D

D

G

H

S

S

I

V

T

K

R

K

M

Y

D

F

E

D

G

Q

Y

V

T

D

V

I

A

S

N
|
N

G

G

P

L

A

D

L

W

S

S

P

L

D

D

G

H

R

K

T

I

L

F

Y

Y

H

Y

T

I

D

D

S

S

A

L

A

S

R

Y

T

T

I

V

H

D

A

A

F

F

D

D

L

Y

D

D

-

L

G

Q

A

T

G

G

R

Q

L

I

S

S

G

N

K

R

R

R

A

I

H

V

I

Y

R

K

F

M

A

E

E

K

A

D

D

E

G

Q

Y

I

P

P

D
|
D

G

G

M

M

T

C

C

I

D

D

A

A

E

E

G

G

G

K

L

L

W

W

V

V

A

A

H

C

W
x
Y

D

N

G

G

R

R

V

V

S

I

R

R

F

L

R

D

P

P

D

E

-

T

G

G

T

K

L

R

D

L

R

Q

A

T

I

V

A

K

L

L

P

P

V

V

S

D

R

K

V

T

T

T

S

S

C

C

V

C

F

F

A

G

G

G

P

K

A

D

L

Y

D

S

R

E

L

M

F

Y

V

V

T

T

T

C

A

A

A

R

H

D

G

G

I

L

G

N

R

A

D

E

G

G

R

L

P

L

D

R

E

Q

P

P

L

D

A

A

G

G

A

N

L

I

F

F

E

K

C

I

D

T

P

G

G

L

G

G

V

V

R

K

G

G

L

I

P

A

P

P

G

Y

R

S

F

Y

G

A

binding calcium ion: E16 (= E17), N152 (= N153), D202 (= D202)
binding Xylitol: E16 (= E17), R99 (= R105), N101 (= N107), E119 (= E127), N152 (= N153), D202 (= D202), Y217 (≠ W217)

4gn9A Mouse smp30/gnl-glucose complex (see paper)
33% identity, 98% coverage: 4:295/299 of query aligns to 3:296/297 of 4gn9A

query
sites
4gn9A

E

K

V

V

R

E

C

C

V

V

W

L

P

R

A

E

R

N

A

Y

L

R

L

C

G

G

E
|
E

G

S

P

P

L

V

W

W

S

E

P

E

E

A

Q

S

G

Q

A

S

V

L

F

L

F

F

V

V

D

D

I

I

H

P

G

-

S

S

R

K

I

I

L

I

R

C

H

R

G

W

L

D

D

T

D

V

G

S

S

N

Q

Q

A

V

D

Q

W

R

A

V

L

A

E

V

D

D

A

A

A

P

C

V

W

S

L

S

V

V

E

A

C

L

A

R

D

Q

G

L

D

G

G
|
G

F

Y

I

V

A

A

G

T

L

I

R

G

S

T

R

K

R

-

V

F

V

C

R

A

L

L

R

N

L
x
W

E
|
E

P

N

G

Q

R

S

A

V

V

F

I

V

A

-

G

-

E

-

L

L

A

A

R

M

I

V

D
|
D

P
x
E

D

D

R

K

P

K

G

N

N

N

R
|
R

L

F

N
|
N

D

D

A

G

K

K

A

V

D

D

A

P

Q

A

G

G

R

R

L

Y

W

F

I

A

G

G

S

T

M

M

D

-

G

A

E

E

E
|
E

T

T

P

A

-

P

-

A

-

V

-

L

-

E

-

R

-

H

S

Q

G

G

A

S

F

L

H
x
Y

R

S

L

L

D

F

P

P

D

D

G

H

S

S

I

V

T

K

R
x
K

M

Y

D

F

E

D

G

Q

Y

V

T

D

V

I

A

S

N
|
N

G

G

P

L

A

D

L

W

S

S

P

L

D

D

G

H

R

K

T

I

L

F

Y

Y

H

Y

T

I

D

D

S
|
S

A

L

A

S

R

Y

T

T

I

V

H

D

A

A

F

F

D

D

L

Y

D

D

-

L

G

Q

A

T

G

G

R

Q

L

I

S

S

G

N

K

R

R

R

A

I

H

V

I

Y

R

K

F

M

A

E

E

K

A

D

D

E

G

Q

Y

I

P

P

D
|
D

G

G

M

M

T

C

C

I

D

D

A

A

E

E

G

G

G

K

L

L

W

W

V

V

A

A

H

C

W
x
Y

D

N

G

G

R

R

V

V

S

I

R

R

F

L

R

D

P

P

D

E

-

T

G

G

T

K

L

R

D

L

R

Q

A

T

I

V

A

K

L

L

P

P

V

V

S

D

R

K

V

T

T

T

S

S

C

C

V

C

F

F

A

G

G

G

P

K

A

D

L

Y

D

S

R

E

L

M

F

Y

V

V

T

T

T

C

A

A

A

R

H

D

G

G

I

L

G

N

R

A

D

E

G

G

R

L

P

L

D

R

E

Q

P

P

L

D

A

A

G

G

A

N

L

I

F

F

E

K

C

I

D

T

P

G

G

L

G

G

V

V

R

K

G

G

L

I

P

A

P

P

G

Y

R

S

F

Y

G

A

binding beta-D-glucopyranose: E16 (= E17), G66 (= G68), W80 (≠ L83), E81 (= E84), D92 (= D98), E93 (≠ P99), R99 (= R105), N101 (= N107), E119 (= E127), Y133 (≠ H134), K143 (≠ R144), N152 (= N153), S168 (= S169), D202 (= D202), Y217 (≠ W217)
binding calcium ion: E16 (= E17), N152 (= N153), D202 (= D202)

4gn8A Mouse smp30/gnl-1,5-ag complex (see paper)
33% identity, 98% coverage: 4:295/299 of query aligns to 3:296/297 of 4gn8A

query
sites
4gn8A

E

K

V

V

R

E

C

C

V

V

W

L

P

R

A

E

R

N

A

Y

L

R

L

C

G

G

E
|
E

G

S

P

P

L

V

W

W

S

E

P

E

E

A

Q

S

G

Q

A

S

V

L

F

L

F

F

V

V

D

D

I

I

H

P

G

-

S

S

R

K

I

I

L

I

R

C

H

R

G

W

L

D

D

T

D

V

G

S

S

N

Q

Q

A

V

D

Q

W

R

A

V

L

A

E

V

D

D

A

A

A

P

C

V

W

S

L

S

V

V

E

A

C

L

A

R

D
x
Q

G

L

D

G

G

G

F

Y

I

V

A

A

G

T

L

I

R

G

S

T

R

K

R

-

V

F

V

C

R

A

L

L

R

N

L

W

E

E

P

N

G

Q

R

S

A

V

V

F

I

V

A

-

G

-

E

-

L

L

A

A

R

M

I

V

D

D

P

E

D

D

R

K

P

K

G

N

N

N

R
|
R

L

F

N
|
N

D

D

A

G

K

K

A

V

D

D

A

P

Q
x
A

G
|
G

R

R

L

Y

W

F

I

A

G

G

S

T

M

M

D

-

G

A

E

E

E
|
E

T

T

P

A

-
x
P

-

A

-

V

-

L

-

E

-

R

-

H

S

Q

G

G

A

S

F

L

H

Y

R

S

L

L

D

F

P

P

D

D

G

H

S

S

I

V

T

K

R

K

M

Y

D

F

E

D

G

Q

Y

V

T

D

V

I

A

S

N
|
N

G

G

P

L

A

D

L

W

S

S

P

L

D

D

G

H

R

K

T

I

L

F

Y
|
Y

H

Y

T

I

D

D

S

S

A

L

A

S

R

Y

T

T

I

V

H

D

A
|
A

F

F

D

D

L

Y

D
|
D

-

L

G
x
Q

A

T

G

G

R

Q

L

I

S

S

G

N

K

R

R
|
R

A

I

H
x
V

I

Y

R

K

F

M

A

E

E

K

A

D

D

E

G

Q

Y

I

P

P

D
|
D

G

G

M

M

T

C

C

I

D

D

A

A

E

E

G

G

G

K

L

L

W

W

V

V

A

A

H

C

W
x
Y

D

N

G

G

R

R

V

V

S

I

R

R

F

L

R

D

P
|
P

D

E

-

T

G

G

T

K

L

R

D

L

R

Q

A

T

I

V

A

K

L

L

P

P

V

V

S

D

R

K

V

T

T

T

S

S

C

C

V

C

F

F

A

G

G

G

P

K

A

D

L

Y

D

S

R

E

L

M

F

Y

V

V

T

T

T

C

A

A

A

R

H

D

G

G

I

L

G

N

R

A

D

E

G

G

R

L

P

L

D

R

E

Q

P

P

L

D

A

A

G

G

A

N

L

I

F

F

E

K

C

I

D

T

P

G

G

L

G

G

V

V

R

K

G

G

L

I

P

A

P

P

G
x
Y

R
x
S

F

Y

G

A

binding 1,5-anhydro-D-glucitol: Q63 (≠ D65), R99 (= R105), N101 (= N107), A108 (≠ Q114), G109 (= G115), E119 (= E127), P122 (vs. gap), N152 (= N153), Y164 (= Y165), A175 (= A176), D179 (= D180), Q181 (≠ G181), R189 (= R189), V191 (≠ H191), D202 (= D202), Y217 (≠ W217), P227 (= P227), Y293 (≠ G292), S294 (≠ R293)
binding calcium ion: E16 (= E17), N152 (= N153), D202 (= D202)

4gn7A Mouse smp30/gnl (see paper)
33% identity, 98% coverage: 4:295/299 of query aligns to 3:296/297 of 4gn7A

query
sites
4gn7A

E

K

V

V

R

E

C

C

V

V

W

L

P

R

A

E

R

N

A

Y

L

R

L

C

G

G

E
|
E

G

S

P

P

L

V

W

W

S

E

P

E

E

A

Q

S

G

Q

A

S

V

L

F

L

F

F

V

V

D

D

I

I

H

P

G

-

S

S

R

K

I

I

L

I

R

C

H

R

G

W

L

D

D

T

D

V

G

S

S

N

Q

Q

A

V

D

Q

W

R

A

V

L

A

E

V

D

D

A

A

A

P

C

V

W

S

L

S

V

V

E

A

C

L

A

R

D

Q

G

L

D

G

G

G

F

Y

I

V

A

A

G

T

L

I

R

G

S

T

R

K

R

-

V

F

V

C

R

A

L

L

R

N

L

W

E

E

P

N

G

Q

R

S

A

V

V

F

I

V

A

-

G

-

E

-

L

L

A

A

R

M

I

V

D

D

P

E

D

D

R

K

P

K

G

N

N

N

R

R

L

F

N

N

D

D

A

G

K

K

A

V

D

D

A

P

Q

A

G

G

R

R

L

Y

W

F

I

A

G

G

S

T

M

M

D

-

G

A

E

E

T

T

P

A

-

P

-

A

-

V

-

L

-

E

-

R

-

H

S

Q

G

G

A

S

F

L

H

Y

R

S

L

L

D

F

P

P

D

D

G

H

S

S

I

V

T

K

R

K

M

Y

D

F

E

D

G

Q

Y

V

T

D

V

I

A

S

N
|
N

G

G

P

L

A

D

L

W

S

S

P

L

D

D

G

H

R

K

T

I

L

F

Y

Y

H

Y

T

I

D

D

S

S

A

L

A

S

R

Y

T

T

I

V

H

D

A

A

F

F

D

D

L

Y

D

D

-

L

G

Q

A

T

G

G

R

Q

L

I

S

S

G

N

K

R

R

R

A

I

H

V

I

Y

R

K

F

M

A

E

E

K

A

D

D

E

G

Q

Y

I

P

P

D
|
D

G

G

M

M

T

C

C

I

D

D

A

A

E

E

G

G

G

K

L

L

W

W

V

V

A

A

H

C

W

Y

D

N

G

G

R

R

V

V

S

I

R

R

F

L

R

D

P

P

D

E

-

T

G

G

T

K

L

R

D

L

R

Q

A

T

I

V

A

K

L

L

P

P

V

V

S

D

R

K

V

T

T

T

S

S

C

C

V

C

F

F

A

G

G

G

P

K

A

D

L

Y

D

S

R

E

L

M

F

Y

V

V

T

T

T

C

A

A

A

R

H

D

G

G

I

L

G

N

R

A

D

E

G

G

R

L

P

L

D

R

E

Q

P

P

L

D

A

A

G

G

A

N

L

I

F

F

E

K

C

I

D

T

P

G

G

L

G

G

V

V

R

K

G

G

L

I

P

A

P

P

G

Y

R

S

F

Y

G

A

binding calcium ion: E16 (= E17), N152 (= N153), D202 (= D202)

5gx1A Luciferin-regenerating enzyme collected with serial synchrotron rotational crystallography with accumulated dose of 1.1 mgy (1st measurement) (see paper)
29% identity, 93% coverage: 12:289/299 of query aligns to 12:300/307 of 5gx1A

query
sites
5gx1A

R

K

A

Y

L

T

L

V

G

G

E
|
E

G

G

P

P

L

H

W

W

S

D

P

H

E

E

Q

T

G

Q

A

T

V

L

F

Y

F

F

V

V

D

D

I

T

H

V

G

E

S

K

R

T

I

F

L

H

R

K

H

Y

G

V

L

P

D

S

D

Q

G

K

S

K

Q

Y

A

T

D

F

W
x
C

A

K

L

V

E

D

D

K

A

L

A

V

C

S

W

F

L

I

V

I

E

P

C

L

A

A

D

G

G

S

D

P

G

G

-

R

F

F

I

V

A

V

G

S

L

L

R

E

S

-

R

R

R

E

V

I

V

A

R

I

L
|
L

R

T

L
x
W

E

D

P

G

G

V

R

S

A

A

V

A

I

P

A

T

G

S

-

I

-

E

E

A

L

I

A

V

R

N

I

V

D

E

P

P

D

H

R

I

P

K

G

N

N

N

R

R

L

L

N

N

D

D

A

G

K

K

A

A

D

D

A

P

Q

L

G

G

R

N

L

L

W

W

I

T

G

G

S

T

M

M

D

A

D

I

G

D

E

A

E

G

T

L

P

P

-

I

-

G

-

P

-

V

S

T

G

G

A

S

F

L

H

Y

R

H

L

L

D

G

P

A

D

D

G

K

S

K

I

V

T

K

R

M

M

H

D

E

E

S

G

N

Y

I

T

A

V

I

A

A

N
|
N

G

G

P

L

A

A

L

W

S

S

P

N

D

D

G

L

R

K

T

K

L

M

Y

Y

H

Y

T

I

D

D

S

S

A

G

A

K

R

R

T

R

I

V

H

D

A

E

F

Y

D

D

L

Y

D

D

G

A

A

S

G

T

-

L

R

S

L

I

S

S

G

N

K

Q

R

R

A

P

H

L

I

F

R

T

F

F

A

E

-

K

-

H

E

E

A

V

D

P

G

G

Y

Y

P

P

D
|
D

G

G

M

Q

T

T

C

I

D

D

A

E

E

E

G

G

G

N

L

L

W

W

V

V

A

A

H

V

W

F

D

Q

G

G

G

Q

R

R

V

I

S

I

R

K

F

I

-

S

-

T

-

Q

R

Q

P

P

D

E

G

V

T

L

L

L

D

D

R

-

A

T

I

V

A

K

L

I

P

P

V

D

S

P

R

Q

V

V

T

T

S

S

C

V

V

A

F

F

A

G

G

G

P

P

A

N

L

L

D

D

R

E

L

L

F

Y

V

V

T

T

T

S

A

A

A

G

H

L

G

Q

I

L

G

D

R

D

D

S

G

S

R

F

P

D

D

K

E

S

P

L

L

V

A

N

G

G

A

H

L

V

F

Y

E

R

C

V

D

T

P

G

G

L

G

G

V

V

R

K

G

G

L

F

binding mercury (ii) ion: C51 (≠ W51), L81 (= L81), W83 (≠ L83), W83 (≠ L83)
binding magnesium ion: E17 (= E17), N159 (= N153), D211 (= D202)

5d9bA Luciferin-regenerating enzyme solved by siras using xfel (refined against native data) (see paper)
29% identity, 93% coverage: 12:289/299 of query aligns to 12:300/307 of 5d9bA

query
sites
5d9bA

R

K

A

Y

L

T

L

V

G

G

E
|
E

G

G

P

P

L

H

W

W

S

D

P

H

E

E

Q

T

G

Q

A

T

V

L

F

Y

F

F

V

V

D

D

I

T

H

V

G

E

S

K

R

T

I

F

L

H

R

K

H

Y

G

V

L

P

D

S

D

Q

G

K

S

K

Q

Y

A

T

D

F

W

C

A

K

L

V

E

D

D

K

A

L

A

V

C

S

W

F

L

I

V

I

E

P

C

L

A

A

D

G

G

S

D

P

G

G

-

R

F

F

I

V

A

V

G

S

L

L

R

E

S

-

R

R

R

E

V

I

V

A

R

I

L

L

R

T

L

W

E

D

P

G

G

V

R

S

A

A

V

A

I

P

A

T

G

S

-

I

-

E

E

A

L

I

A

V

R

N

I

V

D

E

P

P

D

H

R

I

P

K

G

N

N

N

R

R

L

L

N

N

D

D

A

G

K

K

A

A

D

D

A

P

Q

L

G

G

R

N

L

L

W

W

I

T

G

G

S

T

M

M

D

A

D

I

G

D

E

A

E

G

T

L

P

P

-

I

-

G

-

P

-

V

S

T

G

G

A

S

F

L

H

Y

R

H

L

L

D

G

P

A

D

D

G

K

S

K

I

V

T

K

R

M

M

H

D

E

E

S

G

N

Y

I

T

A

V

I

A

A

N
|
N

G

G

P

L

A

A

L

W

S

S

P

N

D

D

G

L

R

K

T

K

L

M

Y

Y

H

Y

T

I

D

D

S

S

A

G

A

K

R

R

T

R

I

V

H

D

A

E

F

Y

D

D

L

Y

D

D

G

A

A

S

G

T

-

L

R

S

L

I

S

S

G

N

K

Q

R

R

A

P

H

L

I

F

R

T

F

F

A

E

-

K

-

H

E

E

A

V

D

P

G

G

Y

Y

P

P

D
|
D

G

G

M

Q

T

T

C

I

D

D

A

E

E

E

G

G

G

N

L

L

W

W

V

V

A

A

H

V

W

F

D

Q

G

G

G

Q

R

R

V

I

S

I

R

K

F

I

-

S

-

T

-

Q

R

Q

P

P

D

E

G

V

T

L

L

L

D

D

R

-

A

T

I

V

A

K

L

I

P

P

V

D

S

P

R

Q

V

V

T

T

S

S

C

V

V

A

F

F

A

G

G

G

P

P

A

N

L

L

D

D

R

E

L

L

F

Y

V

V

T

T

T

S

A

A

A

G

H

L

G

Q

I

L

G

D

R

D

D

S

G

S

R

F

P

D

D

K

E

S

P

L

L

V

A

N

G

G

A

H

L

V

F

Y

E

R

C

V

D

T

P

G

G

L

G

G

V

V

R

K

G

G

L

F

binding magnesium ion: E17 (= E17), N159 (= N153), D211 (= D202)

8dk0A Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound (s)gamma- valerolactone (see paper)
30% identity, 56% coverage: 107:274/299 of query aligns to 115:288/293 of 8dk0A

query
sites
8dk0A

N
|
N

D

D

A

L

K

V

A

F

D

D

A

K

Q

H

G

G

R

G

L

L

W

W

I

F

G

T

S

D

M

L

D

G

D

K

-

R

G

A

E

R

E

D

T

M

P

D

S

V

G

G

A

A

F

A

H

Y

R

Y

L

I

D

K

P

P

D

G

G

M

S

T

-

E

I

I

T

T

R

E

M

Q

D

V

E

F

G

G

Y

T

T

L

V

P

A

L

N
|
N

G

G

P

I

A

G

L

L

S

S

P

P

D

D

G

E

R

A

T

T

L

M

Y

Y

H

A

T

A

D

E

S

T

A

P

A

T

R

G

T

R

I

L

H

W

A

A

F

F

D

D

L

L

D

S

G

G

A

P

G

G

R

E

L

V

S

K

G

P

K

K

R

G

A

K

H

P

I

I

R

C

F

G

A

L

E

G

A

G

D

Y

G

Q

Y

M

P

F

D
|
D

G
x
S

M

L

T

A

C

V

D

E

A

A

E

S

G

G

G

N

L

V

W

C

V

V

A

A

H

T

W

L

D

V

G

S

G

G

R

C

V

I

S

S

R

V

F

I

R

A

P

P

D

D

G

G

T

T

L

L

D

V

R

E

A

Q

I

V

A

P

L

T

P

G

V

D

S

R

R

V

V

T

T
|
T

S

N

C

I

V

A

F

F

A

G

G

G

P

P

A

D

L

L

D

K

R

T

L

A

F

Y

V

I

T

T

T

L

A

S

A

G

H

K

G

G

-

E

-

L

I

I

G

A

R

M

D

D

-

W

G

S

R

R

P

P

D

G

E

L

P

P

L

L

binding calcium ion: N115 (= N107), N164 (= N153), D213 (= D202), S214 (≠ G203)
binding gamma-Valerolactone: D213 (= D202), T254 (= T243)

Sites not aligning to the query:

8djzA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound product (see paper)
30% identity, 56% coverage: 107:274/299 of query aligns to 115:288/293 of 8djzA

query
sites
8djzA

N
|
N

D

D

A

L

K

V

A

F

D

D

A

K

Q

H

G

G

R

G

L

L

W

W

I

F

G

T

S

D

M

L

D

G

D

K

-

R

G

A

E

R

E

D

T

M

P

D

S

V

G

G

A

A

F

A

H

Y

R

Y

L

I

D

K

P

P

D

G

G

M

S

T

-

E

I

I

T

T

R

E

M

Q

D

V

E

F

G

G

Y

T

T

L

V

P

A

L

N
|
N

G

G

P

I

A

G

L

L

S

S

P

P

D

D

G

E

R

A

T

T

L

M

Y

Y

H

A

T

A

D

E

S

T

A

P

A

T

R

G

T

R

I

L

H

W

A

A

F

F

D

D

L

L

D

S

G

G

A

P

G

G

R

E

L

V

S

K

G

P

K

K

R

G

A

K

H

P

I

I

R

C

F

G

A

L

E

G

A

G

D

Y

G

Q

Y

M

P

F

D
|
D

G
x
S

M

L

T

A

C

V

D

E

A

A

E

S

G

G

G

N

L

V

W

C

V

V

A

A

H

T

W

L

D

V

G

S

G

G

R

C

V

I

S

S

R

V

F

I

R

A

P

P

D

D

G

G

T

T

L

L

D

V

R

E

A

Q

I

V

A

P

L

T

P

G

V

D

S

R

R

V

V

T

T

T

S

N

C

I

V

A

F

F

A

G

G

G

P

P

A

D

L

L

D

K

R

T

L

A

F

Y

V

I

T

T

T

L

A

S

A

G

H

K

G

G

-

E

-

L

I

I

G

A

R

M

D

D

-

W

G

S

R

R

P

P

D

G

E

L

P

P

L

L

binding calcium ion: N115 (= N107), N164 (= N153), D213 (= D202), S214 (≠ G203)
binding (4R)-4-hydroxypentanoic acid: N115 (= N107), D213 (= D202)

Sites not aligning to the query:

8djfA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound tetrahedral intermediate (see paper)
30% identity, 56% coverage: 107:274/299 of query aligns to 115:288/293 of 8djfA

query
sites
8djfA

N
|
N

D

D

A

L

K

V

A

F

D

D

A

K

Q

H

G

G

R

G

L

L

W

W

I

F

G

T

S

D

M

L

D

G

D

K

-

R

G

A

E

R

E

D

T

M

P

D

S

V

G

G

A

A

F

A

H

Y

R

Y

L

I

D

K

P

P

D

G

G

M

S

T

-

E

I

I

T

T

R

E

M

Q

D

V

E

F

G

G

Y

T

T

L

V

P

A

L

N
|
N

G

G

P

I

A

G

L

L

S

S

P

P

D

D

G

E

R

A

T

T

L

M

Y

Y

H

A

T

A

D

E

S

T

A

P

A

T

R

G

T

R

I

L

H

W

A

A

F

F

D

D

L

L

D

S

G

G

A

P

G

G

R

E

L

V

S

K

G

P

K

K

R

G

A

K

H

P

I

I

R

C

F

G

A

L

E

G

A

G

D

Y

G

Q

Y

M

P

F

D
|
D

G
x
S

M

L

T

A

C

V

D

E

A

A

E

S

G

G

G

N

L

V

W

C

V

V

A

A

H

T

W

L

D

V

G

S

G

G

R

C

V

I

S

S

R

V

F

I

R

A

P

P

D

D

G

G

T

T

L

L

D

V

R

E

A

Q

I

V

A

P

L

T

P

G

V

D

S

R

R

V

V

T

T

T

S

N

C

I

V

A

F

F

A

G

G

G

P

P

A

D

L

L

D

K

R

T

L

A

F

Y

V

I

T

T

T

L

A

S

A

G

H

K

G

G

-

E

-

L

I

I

G

A

R

M

D

D

-

W

G

S

R

R

P

P

D

G

E

L

P

P

L

L

binding calcium ion: N115 (= N107), N164 (= N153), D213 (= D202), S214 (≠ G203)
binding (5S)-5-methyloxolane-2,2-diol: N115 (= N107), D213 (= D202)

Sites not aligning to the query:

7rizA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound 2-hydroxyquinoline (see paper)
30% identity, 56% coverage: 107:274/299 of query aligns to 120:301/306 of 7rizA

query
sites
7rizA

N
|
N

D

D

A

L

K

V

A

F

D

D

A

K

Q

H

G

G

R

G

L

L

W

W

I

F

G

T

S

D

M

L

D

G

D

K

-

R

G

A

E

R

E

D

T

M

P

D

S

V

G

G

A

A

F

A

H

Y

R

Y

L

I

D

K

P

P

D

G

G

M

S

T

-

E

I

I

T

T

R

E

M

Q

D

V

E

F

G

G

Y

T

T

L

V

P

A

L

N
|
N

G

G

P

I

A

G

L

L

S

S

P

P

D

D

G

E

R

A

T

T

L

M

Y

Y

H

A

T

A

D

E

S

T

A

P

A

T

R

G

T

R

I

L

H

W

A

A

F

F

D

D

L

L

D

S

G

G

A

P

G

G

R

E

L

V

S

K

G

P

K

R

R

D

A

V

H

I

I

Y

R

R

F

G

A

E

E

K

A

G

D

K

-

P

-

I

-

C

-

G

-

L

-

G

G

G

Y

Y

P

Q

-

M

-

F

D
|
D

G
x
S

M

L

T

A

C

V

D

E

A

A

E

S

G

G

G

N

L

V

W

C

V

V

A

A

H

T

W

L

D

V

G

S

G

G

R

C

V

I

S

S

R

V

F

I

R

A

P

P

D

D

G

G

T

T

L

L

D

V

R

E

A

Q

I

V

A

P

L

T

P

G

V

D

S

R

R

V

V

T

T

T

S

N

C

I

V

A

F

F

A

G

G

G

P

P

A

D

L

L

D

K

R

T

L

A

F

Y

V

I

T

T

T

L

A

S

A

G

H

K

G

G

-

E

-

L

I

I

G

A

R

M

D

D

-

W

G

S

R

R

P

P

D

G

E

L

P

P

L

L

binding calcium ion: N120 (= N107), N169 (= N153), D226 (= D202), S227 (≠ G203)
binding quinolin-2(1h)-one: N120 (= N107), D226 (= D202)

Sites not aligning to the query:

3e5zA X-ray structure of the putative gluconolactonase in protein family pf08450. Northeast structural genomics consortium target drr130.
26% identity, 82% coverage: 17:261/299 of query aligns to 30:276/290 of 3e5zA

query
sites
3e5zA

E
|
E

G

G

P

P

L

V

W

Y

S

V

P

P

E

A

Q

R

G

S

A

A

V

V

F

I

F

F

V

S

D

D

I

V

H

R

G

Q

S

N

R

R

I

T

L

W

R

-

H

-

G

A

L

W

D

S

D

D

G

D

S

G

Q

Q

A

L

D

S

W

P

A

E

L

M

E

H

D

P

A

S

A

H

C

H

W

Q

L

N

V

G

E

H

C

C

A

L

D

N

G

K

D

Q

G

G

F

H

I

L

A

I

G

A

L

C

R

S

S

H

-

G

-

L

R

R

V

L

V

E

R

R

L

Q

R

R

L

-

E

E

P

P

G

G

R

-

A

-

V

-

I

-

A

-

G

G

E

E

L

W

A

E

R

S

I

I

D

A

P

D

D

S

R

F

P

E

G

G

N

K

R

K

L

L

N

N

-

S

-

P

-
x
N

D

D

A

V

K

C

A

L

D

A

A

P

Q

D

G

G

R

S

L

L

W

W

I

F

G

S

S

D

M

P

D

T

D

Y

G

G

E

I

E

D

T

K

P

P

S

E

G

E

A

G

F

Y

-

G

-

E

-

M

-

E

-

L

-

P

-

G

-

R

-

W

-

V

H

F

R

R

L

L

D

A

P

P

D

D

G

G

S

T

I

L

T

S

R

A

M

P

D

I

E

R

G

D

Y

R

T

V

V

K

A

P

N
|
N

G

G

P

L

A

A

L

F

S

L

P

P

D

S

G

G

R

N

T

L

L

L

Y

V

H

-

T

S

D

D

S

T

A

G

A

D

R

N

T

A

I

T

H

H

A

R

F

Y

D

C

L

L

D

N

G

A

A

R

G

G

R

E

L

T

S

E

G

Y

K

Q

R

G

A

V

H

H

I

-

R

-

F

F

A

T

E

V

A

E

D

P

G

G

Y

K

P

T

D
|
D

G

G

M

L

T

R

C

V

D

D

A

A

E

G

G

G

G

L

L

I

W

W

V

A

A

S

H

A

W

G

D

D

G

G

G

-

R

-

V

V

S

H

R

V

F

L

R

T

P

P

D

D

G

G

T

D

L

E

D

L

R

G

A

R

I

V

A

L

L

T

P

P

V

Q

S

T

R

T

V

S

T

N

S

L

C

C

V

-

F

F

A

G

G

G

P

P

A

E

L

G

D

R

R

T

L

L

F

Y

V

M

T

T

T

V

A

S

A

T

binding magnesium ion: E30 (= E17), N117 (vs. gap), N174 (= N153), D220 (= D202)

7risA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound phosphate (see paper)
30% identity, 56% coverage: 107:274/299 of query aligns to 111:288/293 of 7risA

query
sites
7risA

N
|
N

D

D

A

L

K

V

A

F

D

D

A

K

Q

H

G

G

R

G

L

L

W

W

I

F

G

T

S

D

M

L

D

G

D

K

-

R

G

A

E

R

E

D

T

M

P

D

S

V

G

G

A

A

F

A

H

Y

R

Y

L

I

D

K

P

P

D

G

G

M

S

T

-

E

I

I

T

T

R

E

M

Q

D

V

E

F

G

G

Y

T

T

L

V

P

A

L

N
|
N

G

G

P

I

A

G

L

L

S

S

P

P

D

D

G

E

R

A

T

T

L

M

Y

Y

H

A

T

A

D

E

S

T

A

P

A

T

R

G

T

R

I

L

H

W

A

A

F

F

D

D

L

L

D

S

G

G

A

P

G

G

R

E

L

V

S

K

-

P

-

R

-

D

G

G

K

E

R

K

A

G

H

K

I

-

R

P

F

I

A

C

E

G

A

L

D

G

G

G

Y

Y

P

Q

-

M

-

F

D
|
D

G
x
S

M

L

T

A

C

V

D

E

A

A

E

S

G

G

G

N

L

V

W

C

V

V

A

A

H

T

W

L

D

V

G

S

G

G

R

C

V

I

S

S

R

V

F

I

R

A

P

P

D

D

G

G

T

T

L

L

D

V

R

E

A

Q

I

V

A

P

L

T

P

G

V

D

S

R

R

V

V

T

T
|
T

S

N

C

I

V

A

F

F

A

G

G

G

P

P

A

D

L

L

D

K

R

T

L

A

F

Y

V

I

T

T

T

L

A

S

A

G

H

K

G

G

-

E

-

L

I

I

G

A

R

M

D

D

-

W

G

S

R

R

P

P

D

G

E

L

P

P

L

L

binding calcium ion: N111 (= N107), N160 (= N153), D213 (= D202), S214 (≠ G203)
binding phosphate ion: N111 (= N107), N160 (= N153), D213 (= D202), T254 (= T243)

Sites not aligning to the query:

3o4pA Dfpase at 0.85 angstrom resolution (h atoms included) (see paper)
27% identity, 56% coverage: 93:258/299 of query aligns to 105:285/314 of 3o4pA

query
sites
3o4pA

E

E

L

I

A

A

R

K

I

K

D

D

P

S

D

E

-

G

R

R

P

R

G

M

N

Q

R

G

L

C

N
|
N

D

D

A

C

K

A

A

F

D

D

A

Y

Q

E

G

G

R

N

L

L

W

W

I

I

G

-

S

T

M

A

D

P

D

A

G

G

E

E

E

V

T

A

P

P

S

A

-

D

-

Y

-

T

-

R

-

S

-

M

-

Q

-

E

-
x
K

-

F

G

G

A

S

F

I

H

Y

R

C

L

F

D

T

P

T

D

D

G

G

S

Q

I

M

T

I

R

Q

M

V

D

D

E

T

G
x
A

Y
x
F

T

Q

V

F

A

P

N
|
N

G

G

P

I

A

A

L

V

-

R

-

H

S

M

P

N

D

D

G

G

R

R

-

P

-

Y

T

Q

L

L

Y

I

H

V

T

A

D
x
E

S

T

A

P

A

T

R

K

T

K

I

L

H

W

A

S

F

Y

D

D

L

I

D

K

G

G

A

P

G

A

R

K

L

I

S

E

G

N

K
|
K

R

K

A

V

-

W

-

G

H

H

I

I

R

P

F

-

A

G

E

T

A

H

D

E

G

G

Y

G

P

A

D
|
D

G

G

M

M

T
x
D

C

F

D

D

A

E

E

D

G

N

L

L

W

L

V

V

A

A

H

N

W
|
W

D

G

G

S

R

H

V

I

S

E

R

V

F

F

R

G

P

P

D

D

G

G

T

G

L

Q

D

P

R

K

A

M

-

R

I

I

A

R

L

C

P

P

V

F

S

E

R
x
K

V

P

T

S

S

N

C
x
L

V
x
H

F

F

A

K

G

-

P

P

A

Q

L

T

D

K

R

T

L

I

F

F

V

V

T

T

active site: N120 (= N107), N175 (= N153), D229 (= D202)
binding calcium ion: N120 (= N107), N175 (= N153), D229 (= D202), D232 (≠ T205), L273 (≠ C245), H274 (≠ V246)
binding 1,2-dimethoxyethane: W244 (= W217), K269 (≠ R241)
binding 2-methoxyethanol: K151 (vs. gap), A170 (≠ G148), F171 (≠ Y149), E194 (≠ D168), K214 (= K188)

Sites not aligning to the query:

active site: 21, 37, 287
binding calcium ion: 21
binding 1,2-dimethoxyethane: 8, 296, 297

Q7SIG4 Diisopropyl-fluorophosphatase; DFPase; EC 3.8.2.2 from Loligo vulgaris (Common European squid) (see 4 papers)
27% identity, 56% coverage: 93:258/299 of query aligns to 105:285/314 of Q7SIG4

query
sites
Q7SIG4

E

E

L

I

A

A

R

K

I

K

D

D

P

S

D

E

-

G

R

R

P

R

G

M

N

Q

R

G

L

C

N
|
N

D
|
D

A

C

K

A

A

F

D

D

A

Y

Q

E

G

G

R

N

L

L

W

W

I

I

G

-

S

T

M

A

D

P

D

A

G

G

E

E

E

V

T

A

P

P

S

A

-

D

-
x
Y

-

T

-
x
R

-

S

-
x
M

-

Q

-

E

-

K

-

F

G

G

A

S

F

I

H

Y

R

C

L

F

D

T

P

T

D

D

G

G

S

Q

I

M

T

I

R

Q

M

V

D

D

E

T

G

A

Y

F

T

Q

V
x
F

A

P

N
|
N

G

G

P

I

A

A

L

V

-

R

-
x
H

S

M

P

N

D

D

G

G

R

R

-

P

-

Y

T

Q

L

L

Y

I

H

V

T

A

D

E

S
x
T

A

P

A

T

R

K

T

K

I

L

H

W

A

S

F

Y

D

D

L

I

D

K

G

G

A

P

G

A

R

K

L

I

S

E

G

N

K

K

R

K

A

V

-

W

-

G

H
|
H

I

I

R

P

F

-

A

G

E

T

A
x
H

D

E

G

G

Y

G

P

A

D
|
D

G

G

M

M

T
x
D

C

F

D

D

A

E

E

D

G
x
N

G

N

L

L

W

L

V

V

A

A

H

N

W
|
W

D

G

G

S

R
x
H

V

I

S

E

R

V

F

F

R

G

P

P

D

D

G

G

T

G

L

Q

D

P

R

K

A

M

-

R

I

I

A

R

L

C

P

P

V

F

S

E

R

K

V

P

T
x
S

S
x
N

C
x
L

V
x
H

F

F

A

K

G

-

P

P

A

Q

L

T

D

K

R

T

L

I

F

F

V

V

T

T

N120 (= N107) mutation to D: 96% decrease in activity. 100% decrease in activity; when associated with N-229.
D121 (= D108) mutation to F: 100% decrease in activity.
Y144 (vs. gap) mutation to S: 8% increase in activity.
R146 (vs. gap) mutation to S: 45% decrease in activity.
M148 (vs. gap) mutation to A: 26% decrease in activity.
F173 (≠ V151) mutation to A: 84% decrease in activity.; mutation to L: 28% decrease in activity.; mutation to S: 68% decrease in activity.; mutation to V: 46% decrease in activity.; mutation to W: 19% decrease in activity.; mutation to Y: 53% decrease in activity.
N175 (= N153) mutation to D: 98% decrease in activity.
H181 (vs. gap) mutation to N: 20% decrease in activity.
T195 (≠ S169) mutation to A: 60% decrease in activity.; mutation to L: 11% decrease in activity.; mutation to V: 3% decrease in activity.
H219 (= H191) mutation to N: 3% increase in activity.
H224 (≠ A197) mutation to N: 14% increase in activity.
D229 (= D202) mutation to N: 100% decrease in activity. Loss of calcium 1 binding. 100% decrease in activity; when associated with D-120.
D232 (≠ T205) binding Ca(2+); mutation to S: 3% increase in activity. 19% decrease in activity; when associated with A-271.
N237 (≠ G210) mutation to S: 4% decrease in activity.
W244 (= W217) mutation to F: 44% decrease in activity.; mutation to H: 27% decrease in activity.; mutation to L: 62% decrease in activity.; mutation to Y: No effect on activity.
H248 (≠ R221) mutation to N: 4% increase in activity.
S271 (≠ T243) mutation to A: 30% increase in activity. 19% decrease in activity; when associated with S-232.
N272 (≠ S244) mutation to F: 100% decrease in activity.
L273 (≠ C245) binding Ca(2+)
H274 (≠ V246) binding Ca(2+); mutation to N: 85% decrease in activity.

Sites not aligning to the query:

21 E→Q: 100% decrease in activity. Loss of calcium 1 binding.
37 E→Q: 50% decrease in activity.
77 Q→F: 100% decrease in activity.; Q→W: No effect on activity.; Q→Y: 6% increase in activity.
287 active site, Proton acceptor; H→A: 90% decrease in activity.; H→F: 36% decrease in activity.; H→L: 21% decrease in activity.; H→N: 97% decrease in activity.; H→Q: 54% decrease in activity.; H→W: 44% decrease in activity.; H→Y: 57% decrease in activity.
304 Q→F: 50% decrease in activity.; Q→W: 3% decrease in activity.
314 F→A: 3% increase in activity.

Query Sequence

>AZOBR_RS31230 FitnessBrowser__azobra:AZOBR_RS31230
MPQEVRCVWPARALLGEGPLWSPEQGAVFFVDIHGSRILRHGLDDGSQADWALEDAACWL
VECADGDGFIAGLRSRRVVRLRLEPGRAVIAGELARIDPDRPGNRLNDAKADAQGRLWIG
SMDDGEETPSGAFHRLDPDGSITRMDEGYTVANGPALSPDGRTLYHTDSAARTIHAFDLD
GAGRLSGKRAHIRFAEADGYPDGMTCDAEGGLWVAHWDGGRVSRFRPDGTLDRAIALPVS
RVTSCVFAGPALDRLFVTTAAHGIGRDGRPDEPLAGALFECDPGGVRGLPPGRFGHPPG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory