SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AZOBR_RS31255 FitnessBrowser__azobra:AZOBR_RS31255 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q53TZ2 L-arabinose 1-dehydrogenase (NAD(P)(+)); D-galactose 1-dehydrogenase; EC 1.1.1.376; EC 1.1.1.120; EC 1.1.1.48 from Azospirillum brasilense (see paper)
51% identity, 96% coverage: 12:323/325 of query aligns to 5:307/309 of Q53TZ2

query
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Q53TZ2

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D169 (= D182) mutation to A: Loss of activity.
N173 (= N186) mutation to A: Decrease by 4 orders of magnitude in catalytic efficiency.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

7cgqA Crystal structure of azospirillum brasilense l-arabinose 1- dehydrogenase e147a mutant (NADP and l-arabinose bound form)
50% identity, 96% coverage: 12:323/325 of query aligns to 2:304/306 of 7cgqA

query
sites
7cgqA

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binding alpha-L-arabinopyranose: K88 (= K104), H116 (= H132), H150 (= H166), P167 (= P183), N170 (= N186)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G20), K11 (= K21), I12 (= I22), S34 (= S44), R35 (≠ P45), H36 (= H46), C64 (= C80), A65 (≠ T81), P66 (= P82), V69 (= V85), E87 (= E103), K88 (= K104), H116 (= H132), Q153 (= Q169), W155 (= W171), D166 (= D182), Y263 (= Y282)

1rydA Crystal structure of glucose-fructose oxidoreductase from zymomonas mobilis
29% identity, 62% coverage: 3:202/325 of query aligns to 17:234/381 of 1rydA

query
sites
1rydA

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active site: K129 (= K104), Y217 (≠ N186)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L39 (≠ F19), G40 (= G20), K41 (= K21), Y42 (≠ I22), S64 (= S44), G65 (≠ P45), K69 (vs. gap), Y87 (≠ R62), L106 (≠ T81), P107 (= P82), N108 (≠ P83), L110 (≠ V85), H111 (≠ R86), E128 (= E103), K129 (= K104), R157 (≠ H132), A196 (≠ G168), W199 (= W171), R200 (≠ I172), Y217 (≠ N186)

Sites not aligning to the query:

1h6dA Oxidized precursor form of glucose-fructose oxidoreductase from zymomonas mobilis complexed with glycerol (see paper)
29% identity, 62% coverage: 3:202/325 of query aligns to 19:236/383 of 1h6dA

query
sites
1h6dA

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active site: K131 (= K104), Y219 (≠ N186)
binding glycerol: K131 (= K104), R202 (≠ I172), D215 (= D182), Y219 (≠ N186)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R19 (= R3), G40 (= G18), L41 (≠ F19), G42 (= G20), K43 (= K21), Y44 (≠ I22), S66 (= S44), G67 (≠ P45), K71 (vs. gap), Y89 (≠ R62), I107 (≠ C80), L108 (≠ T81), P109 (= P82), N110 (≠ P83), H113 (≠ R86), E130 (= E103), K131 (= K104), R159 (≠ H132), A198 (≠ G168), W201 (= W171), R202 (≠ I172), Y219 (≠ N186)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 17, 18, 298

1evjA Crystal structure of glucose-fructose oxidoreductase (gfor) delta1-22 s64d (see paper)
29% identity, 58% coverage: 16:202/325 of query aligns to 7:205/340 of 1evjA

query
sites
1evjA

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F

T

A

V

I

E

R

A

A

L

F

R

K

A

A

G

G

K

K

H

H

V

V

L

M

I

C

E
|
E

K
|
K

P

P

P

M

A

A

A

T

T

S

L

V

T

A

E

D

L

C

R

Q

L

R

L

M

L

I

D

D

A

A

E

K

G

A

A

A

K

N

R

K

T

K

L

L

F

M

A

I

A

G

W

Y

H
x
R

S

C

R

H

F

Y

N

D

-

P

-

M

-

N

-

R

A

A

V

V

E

K

E

L

T

I

R

R

R

E

-

N

-

Q

-

L

-

G

R

K

L

L

A

G

G

M

A

V

T

T

V

T

R

D

N

N

V

S

A

D

I

V

T

M

W

D

K

Q

E

N

D

D

V

-

R

-

W

-

H

-

P

P

G

A

Q

Q

D

Q

W
|
W

I
x
R

F

L

A

R

-

R

-

E

-

L

A

A

G

G

F

G

G

S

V

L

F

M

D

D

P

I

G

G

I

I

N
x
Y

A

G

L

L

S

N

I

G

L

T

T

R

E

Y

I

L

L

L

-

G

P

E

A

E

P

P

V

I

V

E

V

V

R

R

active site: K100 (= K104), Y188 (≠ N186)
binding nicotinamide-adenine-dinucleotide: G9 (= G18), L10 (≠ F19), G11 (= G20), K12 (= K21), Y13 (≠ I22), D35 (≠ S44), L77 (≠ T81), P78 (= P82), N79 (≠ P83), H82 (≠ R86), E99 (= E103), K100 (= K104), R128 (≠ H132), W170 (= W171), R171 (≠ I172), Y188 (≠ N186)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 267

1zh8A Crystal structure of oxidoreductase (tm0312) from thermotoga maritima at 2.50 a resolution
26% identity, 93% coverage: 12:314/325 of query aligns to 4:308/325 of 1zh8A

query
sites
1zh8A

L

I

T

R

L

L

G

G

I

I

V

V

G
|
G

F
x
C

G
|
G

K
x
I

I
x
A

A

A

R

R

D

E

Q

L

H

H

V

L

P

P

A

A

I

L

A

K

A

N

-

L

T

S

G

H

L

L

F

F

R

E

L

I

A

T

A

A

V

V

V

T

S
|
S

P
x
R

H
x
T

G

R

A

S

I
x
H

P

A

E

E

T

F

G

A

-

K

-

M

L

V

G

G

D

N

V

P

A

A

V

V

F

F

R

D

S

S

Q

Y

T

E

E

E

M

L

L

L

A

E

A

S

L

-

P

-

G

G

L

L

-

V

D

D

A

A

V

V

A

D

I

L

C
x
T

T
x
L

P
|
P

P

V

A

E

V
x
L

R

N

H

L

P

P

L

F

T

I

V

E

E

K

A

A

L

L

R

R

A

K

G

G

K

V

H

H

V

V

L

I

I

C

E
|
E

K
|
K

P

P

P

I

A

S

A

T

T

D

L

V

T

E

E

T

L

G

R

K

L

K

L

V

D

E

A

L

A

S

E

E

G

K

A

S

K

E

R

K

T

T

L

V

F

Y

A

I

A

A

W

E

H
x
N

S

F

R

R

F

H

N

V

A

P

A

A

V

F

E

W

E

K

T

A

R

K

R

E

R

L

L

V

-

E

A

S

G

G

A

A

T

I

V

G

R

D

N

P

V

V

A

F

I

M

T

N

W

W

K

Q

-

I

-

W

-

V

-

G

E

M

D

D

V

E

R

N

W

K

H
x
Y

P

V

G

H

Q

T

D

D

W
|
W

I
x
R

F

K

A

K

-

P

-

K

-

H

A

V

G

G

F

F

G

-

V

L

F

S

D

D

P

G

G

G

I

V

N
x
H

A

H

L

A

S

A

-

A

-

M

-

R

-

L

I

I

L

L

T

G

E

E

I

I

L

E

P

W

A

I

P

S

V

A

V

V

V

A

R

K

D

D

A

-

E

-

L

L

R

S

V

P

P

L

A

L

N

G

R

G

D

M

T

D

P

F

I

L

A

S

T

I

L

F

R

E

M

F

E

E

G

N

A

G

A

-

G

-

S

-

A

T

V

V

G

G

T

N

V

Y

T

T

A

I

A

S

F

Y

D

S

F

L

L

-

Q

-

E

K

G

G

E

N

Q

E

T

R

W

F

A

E

I

I

A

-

I

-

E

-

T

-

D

-

S

T

G

G

G

-

L

-

D

-

L

-

T

T

H

K

G

G

G

K

T

I

R

S

L

I

S

S

V

W

D

D

G

K

V

I

L

V

V

L

L

N

A

E

K

E

P

E

-

M

-

K

-

V

-

P

-

Q

D

E

A

N

E

S

Y
|
Y

Q

Q

G

K

I

E

Y

F

R

E

R

D

F

F

H

Y

H

Q

L

V

I

V

A

A

D

E

A

G

A

K

G

P

D

N

V

D

D

L

A

G

R

S

P

P

L

V

Q

Q

L

A

V

L

A

K

D

D

A

L

C

A

L

F

V

I

active site: K98 (= K104), H187 (≠ N186)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G18), C11 (≠ F19), G12 (= G20), I13 (≠ K21), A14 (≠ I22), S37 (= S44), R38 (≠ P45), T39 (≠ H46), H42 (≠ I49), T74 (≠ C80), L75 (≠ T81), P76 (= P82), L79 (≠ V85), E97 (= E103), K98 (= K104), N126 (≠ H132), Y165 (≠ H166), W170 (= W171), R171 (≠ I172), H187 (≠ N186), Y276 (= Y282)

5a05A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with maltotriose (see paper)
31% identity, 55% coverage: 12:189/325 of query aligns to 3:188/335 of 5a05A

query
sites
5a05A

L

L

T

G

L

Y

G

A

I

I

V

L

G
|
G

F
x
L

G
|
G

K
x
Y

I
x
Y

A

A

R

T

D

R

Q

I

H

I

V

M

P

P

A

R

I

F

A

A

A

E

T

C

G

E

L

H

F

S

R

R

L

L

A

A

V

L

V

V

S
|
S

-
x
G

-
x
T

-

P

-

E

P
x
K

H

L

G

K

A

T

I

Y

P

G

E

E

E

Q

T

Y

G

G

L

I

G

P

D

E

V

T

A

H

V

R

F

Y

R

S

S
x
Y

Q

E

T

T

-

F

E

D

M

R

L

I

A

I

A

D

L

N

P

P

G

D

L

V

D

D

A

I

V

V

A

Y

I

V

C
x
I

T
|
T

P
|
P

P
x
N

A

S

V
x
L

R
x
H

H

R

P

P

L

F

T

T

V

E

E

R

A

A

L

A

R

R

A

A

G

G

K

K

H

H

V

V

L

M

I

C

E
|
E

K
|
K

P

P

P

M

A

A

A

N

T

T

L

V

T

A

E

D

L

C

R

E

L

A

L

M

L

I

D

A

A

A

A

C

E

K

G

K

A

A

K

G

R

R

T

K

L

L

F

M

A

I

A

G

W

Y

H
x
R

S

S

R

R

F

F

N

Q

A

A

-

H

A

N

V

I

E

E

E

A

T

I

R

K

R

L

R

V

L

R

A

D

G

G

A

A

T

L

-

G

-

P

V

V

R

R

N

T

V

V

A

V

I

T

T

D

W

H

K

G

-
x
F

-

T

-

I

E

G

D

D

V

P

R

K

R

Q

W
|
W

H
x
R

P

L

G

N

Q

R

D

A

W

L

I

-

F

-

A

-

A

A

G

G

F

G

G

S

V

L

F

M

D
|
D

P

I

G

G

I

I

N
x
Y

A

S

L

L

S

N

active site: K100 (= K104), Y185 (≠ N186)
binding beta-D-glucopyranose: K100 (= K104), F159 (vs. gap), D181 (= D182), Y185 (≠ N186)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G18), L10 (≠ F19), G11 (= G20), Y12 (≠ K21), Y13 (≠ I22), S35 (= S44), G36 (vs. gap), T37 (vs. gap), K40 (≠ P45), Y58 (≠ S63), I76 (≠ C80), T77 (= T81), P78 (= P82), N79 (≠ P83), L81 (≠ V85), H82 (≠ R86), E99 (= E103), K100 (= K104), R128 (≠ H132), W167 (= W165), R168 (≠ H166), Y185 (≠ N186)

Sites not aligning to the query:

5a04A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with glucose (see paper)
31% identity, 55% coverage: 12:189/325 of query aligns to 3:188/335 of 5a04A

query
sites
5a04A

L

L

T

G

L

Y

G

A

I

I

V

L

G

G

F
x
L

G
|
G

K
x
Y

I
x
Y

A

A

R

T

D

R

Q

I

H

I

V

M

P

P

A

R

I

F

A

A

A

E

T

C

G

E

L

H

F

S

R

R

L

L

A

A

V

L

V

V

S
|
S

-
x
G

-
x
T

-

P

-

E

P
x
K

H

L

G

K

A

T

I

Y

P

G

E

E

E

Q

T

Y

G

G

L

I

G

P

D

E

V

T

A

H

V

R

F

Y

R

S

S
x
Y

Q

E

T

T

-

F

E

D

M

R

L

I

A

I

A

D

L

N

P

P

G

D

L

V

D

D

A

I

V

V

A

Y

I

V

C
x
I

T
|
T

P
|
P

P
x
N

A

S

V
x
L

R
x
H

H

R

P

P

L

F

T

T

V

E

E

R

A

A

L

A

R

R

A

A

G

G

K

K

H

H

V

V

L

M

I

C

E
|
E

K
|
K

P

P

P

M

A

A

A

N

T

T

L

V

T

A

E

D

L

C

R

E

L

A

L

M

L

I

D

A

A

A

A

C

E

K

G

K

A

A

K

G

R

R

T

K

L

L

F

M

A

I

A

G

W

Y

H
x
R

S

S

R

R

F

F

N

Q

A

A

-

H

A

N

V

I

E

E

E

A

T

I

R

K

R

L

R

V

L

R

A

D

G

G

A

A

T

L

-

G

-

P

V

V

R

R

N

T

V

V

A

V

I

T

T

D

W

H

K

G

-
x
F

-

T

-

I

E

G

D

D

V

P

R

K

R

Q

W
|
W

H
x
R

P

L

G

N

Q

R

D

A

W

L

I

-

F

-

A

-

A

A

G

G

F

G

G

S

V

L

F

M

D
|
D

P

I

G

G

I

I

N
x
Y

A

S

L

L

S

N

active site: K100 (= K104), Y185 (≠ N186)
binding beta-D-glucopyranose: K100 (= K104), F159 (vs. gap), R168 (≠ H166), D181 (= D182), Y185 (≠ N186)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L10 (≠ F19), G11 (= G20), Y12 (≠ K21), Y13 (≠ I22), S35 (= S44), G36 (vs. gap), T37 (vs. gap), K40 (≠ P45), Y58 (≠ S63), I76 (≠ C80), T77 (= T81), P78 (= P82), N79 (≠ P83), L81 (≠ V85), H82 (≠ R86), E99 (= E103), K100 (= K104), R128 (≠ H132), W167 (= W165), R168 (≠ H166), Y185 (≠ N186)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 263

5a03E Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with xylose (see paper)
31% identity, 55% coverage: 12:189/325 of query aligns to 3:188/335 of 5a03E

query
sites
5a03E

L

L

T

G

L

Y

G

A

I

I

V

L

G
|
G

F
x
L

G
|
G

K
x
Y

I
x
Y

A

A

R

T

D

R

Q

I

H

I

V

M

P

P

A

R

I

F

A

A

A

E

T

C

G

E

L

H

F

S

R

R

L

L

A

A

V

L

V

V

S
|
S

-
x
G

-
x
T

-

P

-

E

P
x
K

H

L

G

K

A

T

I

Y

P

G

E

E

E

Q

T

Y

G

G

L

I

G

P

D

E

V

T

A

H

V

R

F

Y

R

S

S
x
Y

Q

E

T

T

-

F

E

D

M

R

L

I

A

I

A

D

L

N

P

P

G

D

L

V

D

D

A

I

V

V

A

Y

I

V

C
x
I

T
|
T

P
|
P

P
x
N

A

S

V

L

R
x
H

H

R

P

P

L

F

T

T

V

E

E

R

A

A

L

A

R

R

A

A

G

G

K

K

H

H

V

V

L

M

I

C

E
|
E

K
|
K

P

P

P

M

A

A

A

N

T

T

L

V

T

A

E

D

L

C

R

E

L

A

L

M

L

I

D

A

A

A

A

C

E

K

G

K

A

A

K

G

R

R

T

K

L

L

F

M

A

I

A

G

W

Y

H
x
R

S

S

R

R

F

F

N

Q

A

A

-
x
H

A

N

V

I

E

E

E

A

T

I

R

K

R

L

R

V

L

R

A

D

G

G

A

A

T

L

-

G

-

P

V

V

R

R

N

T

V

V

A

V

I

T

T

D

W

H

K

G

-
x
F

-

T

-

I

E

G

D

D

V

P

R

K

R

Q

W
|
W

H
x
R

P

L

G

N

Q

R

D

A

W

L

I

-

F

-

A

-

A

A

G

G

F

G

G

S

V

L

F

M

D
|
D

P

I

G

G

I

I

N
x
Y

A

S

L

L

S

N

active site: K100 (= K104), Y185 (≠ N186)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G18), L10 (≠ F19), G11 (= G20), Y12 (≠ K21), Y13 (≠ I22), S35 (= S44), G36 (vs. gap), T37 (vs. gap), K40 (≠ P45), Y58 (≠ S63), I76 (≠ C80), T77 (= T81), P78 (= P82), N79 (≠ P83), H82 (≠ R86), E99 (= E103), K100 (= K104), R128 (≠ H132), W167 (= W165), R168 (≠ H166), Y185 (≠ N186)
binding beta-D-xylopyranose: K100 (= K104), F159 (vs. gap), R168 (≠ H166), D181 (= D182), Y185 (≠ N186)
binding alpha-D-xylopyranose: H134 (vs. gap)

Sites not aligning to the query:

5a02A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with glycerol (see paper)
31% identity, 55% coverage: 12:189/325 of query aligns to 3:188/335 of 5a02A

query
sites
5a02A

L

L

T

G

L

Y

G

A

I

I

V

L

G

G

F
x
L

G
|
G

K
x
Y

I
x
Y

A

A

R

T

D

R

Q

I

H

I

V

M

P

P

A

R

I

F

A

A

A

E

T

C

G

E

L

H

F

S

R

R

L

L

A

A

V

L

V

V

S
|
S

-
x
G

-
x
T

-

P

-

E

P
x
K

H

L

G

K

A

T

I

Y

P

G

E

E

E

Q

T

Y

G

G

L

I

G

P

D

E

V

T

A

H

V

R

F

Y

R

S

S
x
Y

Q

E

T

T

-

F

E

D

M

R

L

I

A

I

A

D

L

N

P

P

G

D

L

V

D

D

A

I

V

V

A

Y

I

V

C
x
I

T
|
T

P
|
P

P

N

A

S

V
x
L

R
x
H

H

R

P

P

L

F

T

T

V

E

E

R

A

A

L

A

R

R

A

A

G

G

K

K

H

H

V

V

L

M

I

C

E
|
E

K
|
K

P

P

P

M

A

A

A

N

T

T

L

V

T

A

E

D

L

C

R

E

L

A

L

M

L

I

D

A

A

A

A

C

E

K

G

K

A

A

K

G

R

R

T

K

L

L

F

M

A

I

A

G

W

Y

H
x
R

S

S

R

R

F

F

N

Q

A

A

-

H

A

N

V

I

E

E

E

A

T

I

R

K

R

L

R

V

L

R

A

D

G

G

A

A

T

L

-

G

-

P

V

V

R

R

N

T

V

V

A

V

I

T

T

D

W

H

K

G

-

F

-

T

-

I

E

G

D

D

V

P

R

K

R

Q

W
|
W

H
x
R

P

L

G

N

Q

R

D

A

W

L

I

-

F

-

A

-

A

A

G

G

F

G

G

S

V

L

F

M

D

D

P

I

G

G

I

I

N
x
Y

A

S

L

L

S

N

active site: K100 (= K104), Y185 (≠ N186)
binding nadp nicotinamide-adenine-dinucleotide phosphate: L10 (≠ F19), G11 (= G20), Y12 (≠ K21), Y13 (≠ I22), S35 (= S44), G36 (vs. gap), T37 (vs. gap), K40 (≠ P45), Y58 (≠ S63), I76 (≠ C80), T77 (= T81), P78 (= P82), L81 (≠ V85), H82 (≠ R86), E99 (= E103), K100 (= K104), R128 (≠ H132), W167 (= W165), R168 (≠ H166), Y185 (≠ N186)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 263

5a06A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with sorbitol (see paper)
31% identity, 55% coverage: 12:189/325 of query aligns to 4:189/336 of 5a06A

query
sites
5a06A

L

L

T

G

L

Y

G

A

I

I

V

L

G

G

F
x
L

G
|
G

K
x
Y

I
x
Y

A

A

R

T

D

R

Q

I

H

I

V

M

P

P

A

R

I

F

A

A

A

E

T

C

G

E

L

H

F

S

R

R

L

L

A

A

V

L

V

V

S
|
S

-
x
G

-
x
T

-

P

-

E

P
x
K

H

L

G

K

A

T

I

Y

P

G

E

E

E

Q

T

Y

G

G

L

I

G

P

D

E

V

T

A

H

V

R

F

Y

R

S

S
x
Y

Q

E

T

T

-

F

E

D

M

R

L

I

A

I

A

D

L

N

P

P

G

D

L

V

D
|
D

A

I

V

V

A

Y

I

V

C
x
I

T
|
T

P
|
P

P
x
N

A

S

V
x
L

R
x
H

H

R

P

P

L

F

T

T

V

E

E

R

A

A

L

A

R

R

A

A

G

G

K

K

H
|
H

V

V

L

M

I

C

E
|
E

K
|
K

P

P

P

M

A

A

A

N

T

T

L

V

T

A

E

D

L

C

R

E

L

A

L

M

L

I

D

A

A

A

A

C

E

K

G

K

A

A

K

G

R
|
R

T

K

L
|
L

F

M

A

I

A

G

W

Y

H
x
R

S

S

R

R

F

F

N

Q

A

A

-

H

A

N

V

I

E

E

E

A

T

I

R

K

R

L

R

V

L

R

A

D

G

G

A

A

T

L

-

G

-

P

V

V

R

R

N

T

V

V

A

V

I

T

T

D

W

H

K

G

-
x
F

-

T

-

I

E

G

D

D

V

P

R

K

R

Q

W
|
W

H
x
R

P

L

G

N

Q

R

D

A

W

L

I

-

F

-

A

-

A

A

G

G

F

G

G

S

V

L

F

M

D
|
D

P

I

G

G

I

I

N
x
Y

A

S

L

L

S

N

active site: K101 (= K104), Y186 (≠ N186)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L11 (≠ F19), G12 (= G20), Y13 (≠ K21), Y14 (≠ I22), S36 (= S44), G37 (vs. gap), T38 (vs. gap), K41 (≠ P45), Y59 (≠ S63), I77 (≠ C80), T78 (= T81), P79 (= P82), N80 (≠ P83), L82 (≠ V85), H83 (≠ R86), E100 (= E103), K101 (= K104), R129 (≠ H132), W168 (= W165), R169 (≠ H166), Y186 (≠ N186)
binding sorbitol: D72 (= D75), H96 (= H99), K101 (= K104), R122 (= R125), R122 (= R125), L124 (= L127), F160 (vs. gap), R169 (≠ H166), D182 (= D182), Y186 (≠ N186)

Sites not aligning to the query:

5a03C Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with xylose (see paper)
31% identity, 55% coverage: 12:189/325 of query aligns to 4:189/336 of 5a03C

query
sites
5a03C

L

L

T

G

L

Y

G

A

I

I

V

L

G
|
G

F
x
L

G
|
G

K
x
Y

I
x
Y

A

A

R

T

D

R

Q

I

H

I

V

M

P

P

A

R

I

F

A

A

A

E

T

C

G

E

L

H

F

S

R

R

L

L

A

A

V

L

V

V

S
|
S

-
x
G

-
x
T

-

P

-

E

P
x
K

H

L

G

K

A

T

I

Y

P

G

E

E

E

Q

T

Y

G

G

L

I

G

P

D

E

V

T

A

H

V

R

F

Y

R

S

S
x
Y

Q

E

T

T

-

F

E

D

M

R

L

I

A

I

A

D

L

N

P

P

G

D

L

V

D

D

A

I

V

V

A

Y

I

V

C
x
I

T
|
T

P
|
P

P
x
N

A

S

V
x
L

R
x
H

H

R

P

P

L

F

T

T

V

E

E

R

A

A

L

A

R

R

A

A

G

G

K

K

H

H

V

V

L

M

I

C

E
|
E

K
|
K

P

P

P

M

A

A

A

N

T

T

L

V

T

A

E

D

L

C

R

E

L

A

L

M

L

I

D

A

A

A

A

C

E

K

G

K

A

A

K

G

R

R

T

K

L

L

F

M

A

I

A

G

W

Y

H
x
R

S

S

R

R

F

F

N

Q

A

A

-

H

A

N

V

I

E

E

E

A

T

I

R

K

R

L

R

V

L

R

A

D

G

G

A

A

T

L

-

G

-

P

V

V

R

R

N

T

V

V

A

V

I

T

T

D

W

H

K

G

-
x
F

-

T

-

I

E

G

D

D

V

P

R

K

R

Q

W
|
W

H
x
R

P

L

G

N

Q

R

D

A

W

L

I

-

F

-

A

-

A

A

G

G

F

G

G

S

V

L

F

M

D
|
D

P

I

G

G

I

I

N
x
Y

A

S

L

L

S

N

active site: K101 (= K104), Y186 (≠ N186)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G10 (= G18), L11 (≠ F19), G12 (= G20), Y13 (≠ K21), Y14 (≠ I22), S36 (= S44), G37 (vs. gap), T38 (vs. gap), K41 (≠ P45), Y59 (≠ S63), I77 (≠ C80), T78 (= T81), P79 (= P82), N80 (≠ P83), L82 (≠ V85), H83 (≠ R86), E100 (= E103), K101 (= K104), R129 (≠ H132), W168 (= W165), R169 (≠ H166), Y186 (≠ N186)
binding beta-D-xylopyranose: K101 (= K104), F160 (vs. gap), R169 (≠ H166), D182 (= D182), Y186 (≠ N186)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 264

2glxA Crystal structure analysis of bacterial 1,5-af reductase (see paper)
35% identity, 41% coverage: 15:147/325 of query aligns to 4:136/332 of 2glxA

query
sites
2glxA

G

G

I

L

V

I

G
|
G

F
x
A

G
x
S

K
x
T

I
|
I

A

A

R

R

D

E

Q

W

H

V

V

I

P

G

A

A

I

I

A

R

A

A

T

T

G

G

-

G

-

E

L

V

F

V

R

S

L

M

A

M

A
x
S

V
x
T

V

S

S

A

P

E

H
x
R

G

G

A

A

-

A

I

Y

P

A

E

T

E

E

T

N

G

G

L

I

G

G

D

K

V

S

A

V

V

-

F

-

R

-

S

T

Q

S

T

V

E

E

M

E

L

L

A

V

A

G

L

D

P

P

G

D

L

V

D

D

A

A

V

V

A

Y

I

V

C
x
S

T
|
T

P

T

P
x
N

A

E

V

L

R
x
H

H

R

P

E

L

Q

T

T

V

L

E

A

A

A

L

I

R

R

A

A

G

G

K

K

H

H

V

V

L

L

I

C

E
|
E

K
|
K

P

P

P

L

A

A

A

M

T

T

L

L

T

E

E

D

L

A

R

R

L

E

L

M

L

V

D

V

A

A

E

R

G

E

A

A

K

G

R

V

T

V

L

L

F

G

A

T

A

N

W

H

H
|
H

S

L

R

R

F

N

N

A

A

A

V

H

E

R

E

A

T

M

R

R

R

D

R

A

L

I

A

A

active site: K93 (= K104)
binding acetate ion: K93 (= K104)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G18), A8 (≠ F19), S9 (≠ G20), T10 (≠ K21), I11 (= I22), S32 (≠ A41), T33 (≠ V42), R37 (≠ H46), S69 (≠ C80), T70 (= T81), N72 (≠ P83), H75 (≠ R86), E92 (= E103), K93 (= K104), H121 (= H132)

Sites not aligning to the query:

active site: 179
binding acetate ion: 179
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 161, 162, 282

Q2I8V6 1,5-anhydro-D-fructose reductase; Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming); EC 1.1.1.292 from Ensifer adhaerens (Sinorhizobium morelense) (see paper)
35% identity, 41% coverage: 15:147/325 of query aligns to 5:137/333 of Q2I8V6

query
sites
Q2I8V6

G

G

I

L

V

I

G

G

F
x
A

G
x
S

K
x
T

I
|
I

A
|
A

R

R

D

E

Q

W

H

V

V

I

P

G

A

A

I

I

A

R

A

A

T

T

G

G

-

G

-

E

L

V

F

V

R

S

L

M

A

M

A
x
S

V
x
T

V

S

S

A

P

E

H
x
R

G

G

A

A

-

A

I

Y

P

A

E

T

E

E

T

N

G

G

L

I

G

G

D

K

V

S

A

V

V

-

F

-

R

-

S

T

Q

S

T

V

E

E

M

E

L

L

A

V

A

G

L

D

P

P

G

D

L

V

D

D

A

A

V

V

A

Y

I

V

C

S

T
|
T

P
x
T

P
x
N

A
x
E

V
x
L

R
x
H

H

R

P

E

L

Q

T

T

V

L

E

A

A

A

L

I

R

R

A

A

G

G

K

K

H

H

V

V

L

L

I

C

E
|
E

K
|
K

P

P

P

L

A

A

A

M

T

T

L

L

T

E

E

D

L

A

R

R

L

E

L

M

L

V

D

V

A

A

E

R

G

E

A

A

K

G

R

V

T

V

L

L

F

G

A

T

A
x
N

W

H

H

H

S

L

R

R

F

N

N

A

A

A

V

H

E

R

E

A

T

M

R

R

R

D

R

A

L

I

A

A

ASTI 9:12 (≠ FGKI 19:22) binding
S10 (≠ G20) mutation to G: Almost no effect.
A13 (= A23) mutation to G: Can use NAD as cosubstrate as well as NADP.
S33 (≠ A41) mutation to D: No activity.
ST 33:34 (≠ AV 41:42) binding
R38 (≠ H46) binding
TTNELH 71:76 (≠ TPPAVR 81:86) binding
EK 93:94 (= EK 103:104) binding
K94 (= K104) mutation to G: Less than 1% remaining activity.
N120 (≠ A130) binding

Sites not aligning to the query:

162:163 binding
176 D→A: Less than 1% remaining activity.
180 H→A: Less than 2% remaining activity.
206 G→I: No effect.
283 binding

3q2kC Crystal structure of the wlba dehydrogenase from bordetella pertussis in complex with nadh and udp-glcnaca (see paper)
27% identity, 69% coverage: 6:228/325 of query aligns to 2:233/347 of 3q2kC

query
sites
3q2kC

P

P

L

I

P

T

P

D

A

R

P

K

L

I

T

R

L

F

G

G

I

L

V

V

G

G

F

C

G
|
G

K
x
R

I
|
I

A

S

R

K

D

N

Q

-

H

H

V

I

P

G

A

A

I

I

A

A

A

Q

T

H

G

G

L

-

F

-

R

D

L

R

A

A

A

E

V

L

V

V

S

E

P

I

H

C

G
x
D

A
x
T

I

N

P

P

E

E

E

A

T

L

G

Q

L

A

G

A

D

E

V

A

A

A

V

T

-

G

-

A

-

R

-

P

F

F

R

S

S

S

Q

L

T

S

E

D

M

M

L

L

A

A

A

Q

L

-

P

G

G

N

L

A

D

D

A

A

V

L

A

V

I

L

C
x
A

T
|
T

P

P

P
x
S

A

G

V

L

R
x
H

H

P

P

W

L
x
Q

T

A

V

I

E

E

A

V

L

A

R

Q

A

A

G

G

K

R

H

H

V

V

L

V

I

S

E
|
E

K
|
K

P

P

P

M

A

A

A

T

T

R

L

W

T

E

E

D

L

G

R

K

L

R

L

M

L

V

D

K

A

A

A

C

E

D

G

E

A

A

K

G

R

V

T

R

L

L

F

F

A

V

W

K

H
x
Q

S

N

R

R

F

R

N

N

A

A

A

T

V

L

E

Q

E

L

T

V

R

K

-

A

-

I

-

E

-

Q

-

G

R

R

L

F

A

G

G

R

A

I

T

Y

V

M

R

V

N

T

V

V

A

N

I

V

T

F

W
|
W

K
x
T

E
x
R

-

P

-

Q

-

E

-
x
Y

-

Y

D

D

V

A

R

A

R

R

W
|
W

H
x
R

P

G

G

K

Q

W

D

E

W

W

I

-

F

-

A

-

G

-

G

D

F

G

G

G

V

A

F

F

D

M

P
x
N

G
x
Q

I

A

N

S

A
x
H

L

Y

S

V

I

D

L

L

T

L

E

D

I

W

L

L

P

V

A

G

P

P

V

V

V

E

V

S

R

V

D

Y

A

A

E

Y

L

T

R

A

V

T

P

L

A

A

N

R

R

R

-

I

-

E

-

A

-

E

D

D

T

T

P

G

I

V

A

A

T

L

L

R

R

W

M

R

E

H

G

G

A

A

A

M

G

G

S

S

active site: K100 (= K104), H188 (≠ A187)
binding (2S,3S,4R,5R,6R)-5-acetamido-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4-dihydroxy-oxane-2-carboxylic acid: R17 (≠ K21), K100 (= K104), W159 (= W158), T160 (≠ K159), R161 (≠ E160), Y165 (vs. gap), N184 (≠ P183), Q185 (≠ G184), H188 (≠ A187)
binding 1,4-dihydronicotinamide adenine dinucleotide: G16 (= G20), R17 (≠ K21), I18 (= I22), D40 (≠ G47), T41 (≠ A48), A76 (≠ C80), T77 (= T81), S79 (≠ P83), H82 (≠ R86), Q85 (≠ L89), E99 (= E103), K100 (= K104), Q128 (≠ H132), W171 (= W165), R172 (≠ H166)

Sites not aligning to the query:

binding (2S,3S,4R,5R,6R)-5-acetamido-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4-dihydroxy-oxane-2-carboxylic acid: 243, 244

3m2tA The crystal structure of dehydrogenase from chromobacterium violaceum
28% identity, 41% coverage: 12:143/325 of query aligns to 4:136/342 of 3m2tA

query
sites
3m2tA

L

I

T

K

L

V

G

G

I

L

V

V

G
|
G

F

I

G
|
G

K
x
A

I
x
Q

A

M

R

Q

D

E

Q

N

H

L

V

L

P

P

A

S

I

L

A

L

A

Q

T

M

G

Q

L

D

F

I

R

R

L

I

A

V

A

A

V

A

V

C

S
x
D

P
x
S

-

D

-

L

H

E

G
x
R

A

A

I

R

P

R

E

V

E

H

T

R

G

F

L

I

G

S

D

D

V

I

A

P

V

V

F

L

R

D

S

N

Q

V

T

P

E

A

M

M

L

L

A

N

A

Q

L

V

P

P

G

-

L

L

D

D

A

A

V

V

A

V

I

M

C
x
A

T
x
G

P

P

A

Q

V
x
L

R

H

H

F

P

E

L

M

T

G

V

L

E

L

A

A

L

M

R

S

A

K

G

G

K

V

H

N

V

V

L

F

I

V

E
|
E

K
|
K

P

P

A

C

A

A

T

T

L

L

T

E

E

E

L

L

R

E

L

T

L

L

L

I

D

D

A

A

E

R

G

R

A

S

K

D

R

V

T

V

L

S

F

G

A

V

A

G

W

M

H
x
N

S

F

R

K

F

F

N

A

A

R

A

P

V

V

E

R

E

Q

T

L

R

R

binding nicotinamide-adenine-dinucleotide: G10 (= G18), G12 (= G20), A13 (≠ K21), Q14 (≠ I22), D36 (≠ S44), S37 (≠ P45), R41 (≠ G47), A73 (≠ C80), G74 (≠ T81), L78 (≠ V85), E96 (= E103), K97 (= K104), N125 (≠ H132)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 290

7xr9A Crystal structure of dgpa with glucose (see paper)
25% identity, 52% coverage: 23:190/325 of query aligns to 16:181/344 of 7xr9A

query
sites
7xr9A

A

A

R

N

D

Q

Q

K

H

H

V

F

P

P

A

A

I

L

A

K

A

N

T

N

G

A

L

-

F

-

R

D

L

L

A

N

A

E

V

I

V

V

S

A

P

E

H

K

G

A

A

A

I

A

P

E

E

-

T

-

G

-

L

F

G

G

D

E

V

G

A

A

V

Q

F

V

R

T

S

A

Q
x
D

T
x
Y

E

K

M

E

L

L

A

L

A

A

L

N

P

P

G

E

L

V

D

E

A

V

V

V

A

H

I

V

C
|
C

T
|
T

P
|
P

P

N

A

V

V

S

R
x
H

H

S

P

E

L

I

T

T

V

I

E

A

A

A

L

F

R

E

A

A

G

G

K

K

H

H

V

V

L

Y

I

C

E
|
E

K
|
K

P
|
P

P

M

A

S

A

H

T

S

L

T

T

E

E

E

L

A

R

E

L

K

L

M

L

V

D

E

A

A

A

W

E

K

G

K

A

S

K

G

R

K

T

Q

L

F

F

T

A

I

A

G

W

Y

H
x
Q

S

N

R

R

F

F

N

R

A

E

V

V

E

M

E

N

T

L

R

K

R

S

L

C

A

D

G

K

A

G

T

E

V

L

R

G

N

E

V

I

A

Y

I

Y

T

G

W

K

K

A

E

H

D

A

V

V

R
|
R

R

R

-

R

-

A

-

V

-

P

-

T

W
|
W

H

G

P
x
V

G
x
F

Q

M

D

D

W

-

I

K

F

E

A

A

A
x
Q

G

G

F

G

G

P

V

L

F

I

D
|
D

P

I

G

G

I

T

N
x
H

A

A

L

L

S

D

I

I

binding beta-D-glucopyranose: K91 (= K104), R150 (= R163), W157 (= W165), F160 (≠ G168), D173 (= D182), H177 (≠ N186)
binding nicotinamide-adenine-dinucleotide: D51 (≠ Q64), Y52 (≠ T65), C67 (= C80), T68 (= T81), P69 (= P82), H73 (≠ R86), E90 (= E103), K91 (= K104), P92 (= P105), Q119 (≠ H132), V159 (≠ P167), F160 (≠ G168), Q166 (≠ A175)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 13, 14, 15

3q2kK Crystal structure of the wlba dehydrogenase from bordetella pertussis in complex with nadh and udp-glcnaca (see paper)
27% identity, 67% coverage: 12:228/325 of query aligns to 3:228/322 of 3q2kK

query
sites
3q2kK

L

I

T

R

L

F

G

G

I

L

V

V

G
|
G

F

C

G
|
G

K
x
R

I
|
I

A

S

R

K

D

N

Q

-

H

H

V

I

P

G

A

A

I

I

A

A

A

Q

T

H

G

G

L

-

F

-

R

D

L

R

A

A

A

E

V

L

V

V

S

E

P

I

H

C

G
x
D

A
x
T

I

N

P

P

E

E

E

A

T

L

G

Q

L

A

G

A

D

E

V

A

A

A

V

T

-

G

-

A

-

R

-

P

F

F

R

S

S

S

Q

L

T

S

E

D

M

M

L

L

A

A

A

Q

L

-

P

G

G

N

L

A

D

D

A

A

V

L

A

V

I

L

C

A

T
|
T

P
|
P

P
x
S

A

G

V
x
L

R
x
H

H

P

P

W

L

Q

T

A

V

I

E

E

A

V

L

A

R

Q

A

A

G

G

K

R

H

H

V

V

L

V

I

S

E
|
E

K
|
K

P

P

P

M

A

A

A

T

T

R

L

W

T

E

E

D

L

G

R

K

L

R

L

M

L

V

D

K

A

A

A

C

E

D

G

E

A

A

K

G

R

V

T

R

L

L

F

F

A

V

W

K

H
x
Q

S

N

R

R

F

R

N

N

A

A

A

T

V

L

E

Q

E

L

T

V

R

K

-

A

-

I

-

E

-

Q

-

G

R

R

L

F

A

G

G

R

A

I

T

Y

V

M

R

V

N

T

V

V

A

N

I

V

T

F

W

W

K
x
T

E
x
R

-

P

-

Q

-

E

-
x
Y

-

Y

D

D

V

A

R

A

R

R

W
|
W

H
x
R

P

G

G

K

Q

W

D

E

W

W

I

-

F

-

A

-

G

-

G

D

F

G

G

G

V

A

F

F

D

M

P
x
N

G
x
Q

I

A

N

S

A
x
H

L

Y

S

V

I

D

L

L

T

L

E

D

I

W

L

L

P

V

A

G

P

P

V

V

V

E

V

S

R

V

D

Y

A

A

E

Y

L

T

R

A

V

T

P

L

A

A

N

R

R

R

-

I

-

E

-

A

-

E

D

D

T

T

P

G

I

V

A

A

T

L

L

R

R

W

M

R

E

H

G

G

A

A

A

M

G

G

S

S

active site: K95 (= K104), H183 (≠ A187)
binding (2S,3S,4R,5R,6R)-5-acetamido-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4-dihydroxy-oxane-2-carboxylic acid: R12 (≠ K21), K95 (= K104), T155 (≠ K159), R156 (≠ E160), Y160 (vs. gap), N179 (≠ P183), Q180 (≠ G184), H183 (≠ A187)
binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G18), G11 (= G20), R12 (≠ K21), I13 (= I22), D35 (≠ G47), T36 (≠ A48), T72 (= T81), P73 (= P82), S74 (≠ P83), L76 (≠ V85), H77 (≠ R86), E94 (= E103), K95 (= K104), Q123 (≠ H132), W166 (= W165), R167 (≠ H166), H183 (≠ A187)

Sites not aligning to the query:

binding (2S,3S,4R,5R,6R)-5-acetamido-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4-dihydroxy-oxane-2-carboxylic acid: 239

6jw8A The crystal structure of kand2 in complex with nadh and 3"-deamino-3"- hydroxykanamycin b (see paper)
29% identity, 85% coverage: 11:286/325 of query aligns to 1:271/342 of 6jw8A

query
sites
6jw8A

P

P

L

V

T

R

L

V

G

G

I

V

V

V

G

G

F

A

G
|
G

K
x
F

I
x
M

A

G

R

-

D

G

Q

V

H

H

V

A

P

E

A

V

I

V

A

A

T

H

G

P

L

G

F

A

R

R

L

L

A

E

A

A

V

V

V

-

S

-

P

-

H

H

G
x
D

A
x
L

I

D

P

P

E

-

T

A

G

A

L

A

G

R

D

D

V

L

A

A

V

E

-

R

F

F

R

R

S

A

Q

E

T

R

E

A

M

E

-

P

-

S

-

W

-

A

-

D

L

L

A

L

A

A

L

D

P

P

G

A

L

I

D

D

A

L

V

L

A

I

I

I

C
x
T

T
|
T

P
|
P

P
x
N

A

G

V
x
L

R
x
H

H

H

P

R

L
x
Q

T

A

V

A

E

E

A

A

L

L

R

R

A

A

G

G

K

K

H

H

V

V

L

L

I

V

E
|
E

K
|
K

P

P

P

L

A

G

A

V

T

T

L

P

T

E

E

Q

L

V

R

A

L

E

L

L

L

V

D

E

A

L

A

A

E

G

G

R

A

H

K

D

R

R

T

V

L

L

F

A

A

H

A

G

W

S

H
x
N

S

F

R

V

F

H

N

S

A

P

A

K

V

F

E

V

E

R

T

A

R

R

R

Q

R

L

L

V

A

A

G

D

A

T

T

E

V

A

R

F

N

G

V

R

A

P

I

H

T

L

W

V

K

R

E

V

D

V

V
x
F

R

R

R
x
N

W

S

H

G

P

P

G

E

Q

A

D

A

W
|
W

I

A

F

A

A

S

-

K

-

D

-

L

A

A

G

G

F

G

G

A

V

L

F

L

D
|
D

P
x
L

G

G

I

C

N
x
H

A

A

L

V

S

E

I

L

L

C

T

R

E

W

I

L

L

L

P

D

A

G

-

A

-

D

-

V

-

E

P

S

V

V

V

S

V

A

R

R

D

L

A

Q

E

R

L

V

R

R

V

P

P

P

A

A

N

L

R

E

D

D

T

Q

P

-

I

-

A

-

A

A

T

L

L

L

R

V

M

M

E

E

G

F

A

A

A

D

G

G

S

-

A

A

V

V

G

G

T

Q

V

C

T

D

A

V

A

S

F

W

D

-

F

V

L

T

Q

Q

E

G

G

G

E
|
E

Q

Q

T

V

W

T

A

A

I

E

A

I

I

I

E

G

T

T

D

K

S

G

G

-

L

-

D

-

L

-

T

-

H

-

G

-

T

-

R

R

L

V

S

E

V

V

D

D

-

L

-

W

-

T

G

G

V

M

L

G

V

L

L

R

A

A

K

Y

P

S

D

D

A

K

E

G

Y

Y

Q

Q

G

D

I

V

Y
x
W

binding (2S,3R,4S,5S,6R)-2-[(1S,2S,3R,4S,6R)-3-[(2R,3R,4R,5S,6R)-6-(aminomethyl)-3-azanyl-4,5-bis(oxidanyl)oxan-2-yl]oxy-4,6-bis(azanyl)-2-oxidanyl-cyclohexyl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol: F11 (≠ K21), F152 (≠ V162), N154 (≠ R164), D175 (= D182), L176 (≠ P183), H179 (≠ N186), E236 (= E244), W271 (≠ Y286)
binding 1,4-dihydronicotinamide adenine dinucleotide: G10 (= G20), F11 (≠ K21), M12 (≠ I22), D33 (≠ G47), L34 (≠ A48), T70 (≠ C80), T71 (= T81), P72 (= P82), N73 (≠ P83), L75 (≠ V85), H76 (≠ R86), Q79 (≠ L89), E93 (= E103), K94 (= K104), N122 (≠ H132), W161 (= W171), H179 (≠ N186)

Sites not aligning to the query:

binding 1,4-dihydronicotinamide adenine dinucleotide: 290

6jw7A The crystal structure of kand2 in complex with nadh and 3"-deamino-3"- hydroxykanamycin a (see paper)
29% identity, 85% coverage: 11:286/325 of query aligns to 1:271/342 of 6jw7A

query
sites
6jw7A

P

P

L

V

T

R

L

V

G

G

I

V

V

V

G
|
G

F

A

G
|
G

K
x
F

I
x
M

A

G

R

-

D

G

Q

V

H

H

V

A

P

E

A

V

I

V

A

A

T

H

G

P

L

G

F

A

R

R

L

L

A

E

A

A

V

V

V

-

S

-

P

-

H

H

G
x
D

A
x
L

I

D

P

P

E

-

T

A

G

A

L

A

G

R

D

D

V

L

A

A

V

E

-

R

F

F

R

R

S

A

Q

E

T

R

E

A

M

E

-

P

-

S

-

W

-

A

-

D

L

L

A

L

A

A

L

D

P

P

G

A

L

I

D

D

A

L

V

L

A

I

I

I

C
x
T

T
|
T

P
|
P

P
x
N

A

G

V
x
L

R
x
H

H

H

P

R

L
x
Q

T

A

V

A

E

E

A

A

L

L

R

R

A

A

G

G

K

K

H

H

V

V

L

L

I

V

E
|
E

K
|
K

P

P

P

L

A

G

A

V

T

T

L

P

T

E

E

Q

L

V

R

A

L

E

L

L

L

V

D

E

A

L

A

A

E

G

G

R

A

H

K

D

R

R

T

V

L

L

F

A

A

H

A

G

W

S

H
x
N

S

F

R

V

F

H

N

S

A

P

A

K

V

F

E

V

E

R

T

A

R

R

R

Q

R

L

L

V

A

A

G

D

A

T

T

E

V

A

R

F

N

G

V

R

A

P

I

H

T

L

W

V

K

R

E

V

D

V

V

F

R

R

R
x
N

W

S

H

G

P

P

G

E

Q

A

D

A

W
|
W

I

A

F

A

A

S

-

K

-

D

-

L

A

A

G

G

F

G

G

A

V

L

F

L

D
|
D

P

L

G

G

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binding (2R,3S,4S,5R,6R)-2-(aminomethyl)-6-[(1R,2S,3S,4R,6S)-4,6-bis(azanyl)-3-[(2S,3R,4S,5S,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-2-oxidanyl-cyclohexyl]oxy-oxane-3,4,5-triol: F11 (≠ K21), N154 (≠ R164), D175 (= D182), H179 (≠ N186), E236 (= E244), W271 (≠ Y286)
binding 1,4-dihydronicotinamide adenine dinucleotide: G8 (= G18), G10 (= G20), F11 (≠ K21), M12 (≠ I22), D33 (≠ G47), L34 (≠ A48), T70 (≠ C80), T71 (= T81), P72 (= P82), N73 (≠ P83), L75 (≠ V85), H76 (≠ R86), Q79 (≠ L89), E93 (= E103), K94 (= K104), N122 (≠ H132), W161 (= W171)

Sites not aligning to the query:

binding 1,4-dihydronicotinamide adenine dinucleotide: 290

Query Sequence

>AZOBR_RS31255 FitnessBrowser__azobra:AZOBR_RS31255
MTRPTPLPPAPLTLGIVGFGKIARDQHVPAIAATGLFRLAAVVSPHGAIPEETGLGDVAV
FRSQTEMLAALPGLDAVAICTPPAVRHPLTVEALRAGKHVLIEKPPAATLTELRLLLDAA
EGAKRTLFAAWHSRFNAAVEETRRRLAGATVRNVAITWKEDVRRWHPGQDWIFAAGGFGV
FDPGINALSILTEILPAPVVVRDAELRVPANRDTPIAATLRMEGAAGSAVGTVTAAFDFL
QEGEQTWAIAIETDSGGLDLTHGGTRLSVDGVLVLAKPDAEYQGIYRRFHHLIADAAGDV
DARPLQLVADACLVGRWRTTDAFHW

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory