SitesBLAST

P13650 Quinoprotein glucose dehydrogenase B; Glucose dehydrogenase B [pyrroloquinoline-quinone]; Soluble glucose dehydrogenase; s-GDH; EC 1.1.5.2 from Acinetobacter calcoaceticus (see paper)
28% identity, 95% coverage: 6:371/386 of query aligns to 10:448/478 of P13650

query
sites
P13650

A

A

L

L

M

L

T

S

A

A

T

V

T

Q

L

L

A

V

A

T

C

L

L

S

S

A

V

F

T

A

D

D

A

V

S

P

A

L

V

T

D

P

K

S

V

Q

F

F

-

A

K

K

T

A

E

K

K

S

A

E

M

N

V

F

S

D

V

K

K

K

T

V

V

I

A

L

S

S

G

N

L

L

S

N

H

K

P

P

W

H

G

A

L

L

A

L

F

W

L

G

P

P

D

D

G

N

R

Q

M

I

L

W

V

L

T

T

E

E

K

R

-

A

D

T

G

G

R

K

L

I

R

L

I

R

V

V

A

N

P

P

D

E

G

S

T

G

V

S

S

V

A

K

P

T

V

V

K

F

G

Q

V

V

P

P

K

E

V

I

-

V

-

N

D

D

D

A

R

D

G

G

Q
|
Q

G

N

G

G

L

L

D

G

V

F

A

A

L

F

D

H

P

P

E

D

F

F

A

K

Q

N

N

N

R

P

F

Y

V

I

Y

Y

L

I

S

S

-

G

-

T

F

F

S

K

E

N

P

P

G

K

T

S

E

T

D

D

G

K

T

E

-

L

-

P

N

N

S

Q

T

T

A

I

V

I

A

R

R

R

G

Y

V

T

L

Y

N

N

A

K

D

S

E

T

T

D

A

T

L

L

T

E

D

K

V

P

R

V

V

D

I

L

F

L

S

A

Q

G

K

L

P

P

K

-

V

-

E

S

S

S

R

K

M
x
D

H

H

Y

Q

G

S

S

G

R

R

L

L

V

V

F

I

D

G

R

P

Q

D

G

Q

H

K

L

I

Y

Y

V

Y

T

T

L

I

G

G

E

D

R

Q

S

G

L

R

E

N

Q
|
Q

F

L

R

A

-

Y

-

L

-

F

-

L

-

P

T

N

Q

Q

A

A

Q

Q

-

H

-

T

-

P

-

T

-

Q

-

E

-

L

-

N

-

G

-

K

D

D

L

Y

D

H

S

T

H

Y

L

M

G

G

K

K

V

V

V

L

R

R

I

L

N

N

R

L

D

D

G

G

S

S

V

I

P

P

A

K

D

D

N

N

P

P

F

S

V

F

N

N

Q

-

S

-

G

G

A

V

L

V

P

S

E

H

I

I

W

Y

S

T

Y

L

G

G

H

H

R
|
R

N

N

I

P

Q

Q

G

G

A

L

A

A

L

F

N

T

P

P

Q

N

T

-

G

G

A

K

L

L

W

L

I

Q

N

S

E

E

H

Q

G
|
G

P
|
P

R

N

G

S

G

D

D

D

E
|
E

V

I

N

N

V

L

P

I

E

V

P

K

G

G

K

G

N

N

Y
|
Y

G

G

W

W

P

P

V

N

V

V

S
x
A

-

G

-
x
Y

-

K

-
x
D

-

D

-

S

-

G

-

Y

-

A

-

Y

-

A

-

N

-

Y

-

S

-

A

-

N

-

K

-

S

-

I

Y

K

G

D

V

L

N

A

Y

Q

N

N

G

G

T

V

P

K

I

V

G

A

-

A

-

G

-

V

-

P

-

V

-

T

-

K

-
x
E

-

S

-

E

-

W

T

T

G

G

K

K

S

N

S

F

A

V

P

P

G

P

M

L

E

K

-

T

-

L

-

Y

-

T

-

V

-

Q

-

D

-

T

-

Y

-

N

-

Y

-

N

-

D

-

P

-

T

-

C

-

G

E

E

P

M

V

T

Y
|
Y

Q

I

W

C

T

W

P

P

V
x
T

I

V

G

A

S

P

S

S

G

S

M

A

T

Y

F

V

Y

Y

T

K

A

G

-

G

-

K

D

K

T

A

L

I

P

T

G

G

W

W

K

E

G

N

S

T

L

L

F

L

N

V

G

P

G

S

L

L

A
x
K

T

R

K

G

E

V

V

I

V

F

R

R

L

I

E

K

L

L

D

D

-

P

-

T

-

Y

G

S

N

T

K

T

V

Y

K

D

H

D

E

A

E

V

R

P

M

M

F

F

R

K

D

S

L

-

G

N

K

N

R

R

I

Y

R

R

Q

D

V

V

S

I

Q

A

G

S

P

P

D

D

G

G

-

N

A

V

L

L

Y

Y

L

V

L

L

T

T

D

D

Q100 (= Q92) binding
D167 (≠ M157) binding
Q192 (= Q182) binding
R252 (= R228) binding
G271 (= G248) binding
P272 (= P249) binding
E277 (= E254) binding
Y287 (= Y264) binding
A293 (≠ S270) binding
Y295 (vs. gap) binding
D297 (vs. gap) binding
E333 (vs. gap) binding
Y367 (= Y295) binding
T372 (≠ V300) binding
K401 (≠ A327) binding

1cruA Soluble quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus in complex with pqq and methylhydrazine (see paper)
29% identity, 86% coverage: 39:371/386 of query aligns to 20:422/448 of 1cruA

query
sites
1cruA

K

K

T

V

V

I

A

L

S

S

G

N

L

L

S

N

H

K

P

P

W

H

G

A

L

L

A

L

F

W

L

G

P

P

D

D

G

N

R

Q

M

I

L

W

V

L

T

T

E

E

K

R

-

A

D

T

G

G

R

K

L

I

R

L

I

R

V

V

A

N

P

P

D

E

G

S

T

G

V

S

S

V

A

K

P

T

V

V

K

F

G

Q

V

V

P

P

K

E

V

I

-

V

-

N

D

D

D

A

R

D

G

G

Q
|
Q

G

N

G

G

L

L

D

G

V

F

A

A

L

F

D

H

P

P

E

D

F

F

A

K

Q

N

N

N

R

P

F

Y

V

I

Y

Y

L

I

S

S

-

G

-

T

F

F

S

K

E

N

P

P

G

K

T

S

E

K

D

E

G

L

T

P

N

N

S

Q

T

T

A

I

V

I

A

R

R

R

G

Y

V

T

L

Y

N

N

A

K

D

S

E

T

T

D

A

T

L

L

T

E

D

K

V

P

R

V

V

D

I

L

F

L

S

A

Q

G

K

L

P

P

K

-

V

-

E

S

S

S

R

K

M

D

H
|
H

Y

Q

G

S

S

G

R

R

L

L

V

V

F

I

D

G

R

P

Q

D

G

Q

H

K

L

I

Y

Y

V

Y

T

T

L

I

G

G

E
x
D

R

Q

S

G

L

R

E

N

Q

Q

F

L

R

A

-

Y

-

L

-

F

-

L

-

P

T

N

Q

Q

A

A

Q

Q

-

H

-

T

-

P

-

T

-

Q

-

E

-

L

-

N

-

G

-

K

D

D

L

Y

D

H

S

T

H

Y

L

M

G

G

K

K

V

V

V

L

R

R

I

L

N

N

R

L

D

D

G

G

S

S

V

I

P

P

A

K

D

D

N

N

P

P

F

S

V

F

N

N

Q

-

S

-

G

G

A

V

L

V

P

S

E

H

I

I

W

Y

S

T

Y

L

G

G

H

H

R
|
R

N
|
N

I

P

Q

Q

G

G

A

L

A

A

L

F

N

T

P

P

Q

N

T

-

G

G

A

K

L

L

W

L

I

Q

N

S

E

E

H
x
Q

G
|
G

P
|
P

R

N

G

S

G

D

D

D

E
|
E

V

I

N

N

V

L

P

I

E

V

P

K

G

G

K

G

N

N

Y
|
Y

G

G

W

W

P

P

V

N

V

V

S
x
A

-

G

-
x
Y

-

K

-
x
D

-

D

-

S

-

G

-

Y

-

A

-

Y

-

A

-

N

-

Y

-

S

-

A

-

N

-

K

-

S

-

I

Y

K

G

D

V

L

N

A

Y

Q

N

N

G

G

T

V

P

K

I

V

G

A

-

A

-

G

-

V

-

P

-

V

-

T

-

K

-
x
E

-

S

-

E

-

W

T

T

G

G

K

K

S

N

S

F

A

V

P

P

G

P

M

L

E

K

-

T

-

L

-

Y

-

T

-

V

-

Q

-

D

-

T

-

Y

-

N

-

Y

-

N

-

D

-

P

-

T

-

C

-

G

E

E

P

M

V

T

Y
|
Y

Q

I

W

C

T
x
W

P

P

V
x
T

I

V

G
x
A

S

P

S

S

G

S

M

A

T

Y

F

V

Y

Y

T

K

A

G

-

G

-

K

D

K

T

A

L

I

P

T

G

G

W

W

K

E

G

N

S

T

L

L

F

L

N

V

G

P

G

S

L
|
L

A
x
K

T

R

K

G

E

V

V

I

V

F

R

R

L

I

E

K

L

L

D

D

-

P

-

T

-

Y

G

S

N

T

K

T

V

Y

K

D

H

D

E

A

E

V

R

P

M

M

F

F

R

K

D

S

L

-

G

N

K

N

R
|
R

I

Y

R
|
R

Q

D

V

V

S

I

Q

A

G

S

P

P

D

D

G

G

-

N

A

V

L

L

Y

Y

L

V

L

L

T

T

D

D

active site: H142 (= H158), D161 (≠ E177), R226 (= R228), A267 (≠ S270), Y269 (vs. gap), D271 (vs. gap), E307 (vs. gap)
binding calcium ion: G245 (= G248), P246 (= P249), E251 (= E254), Y261 (= Y264), A267 (≠ S270), Y269 (vs. gap), D271 (vs. gap), E307 (vs. gap)
binding methylhydrazine: Y341 (= Y295), W344 (≠ T298)
binding pyrroloquinoline quinone: Q76 (= Q92), H142 (= H158), R226 (= R228), N227 (= N229), Q244 (≠ H247), Y341 (= Y295), T346 (≠ V300), A348 (≠ G302), L374 (= L326), K375 (≠ A327), R404 (= R354), R406 (= R356)

5minB Apo form of the soluble pqq-dependent glucose dehydrogenase from acinetobacter calcoaceticus
29% identity, 86% coverage: 39:371/386 of query aligns to 20:424/453 of 5minB

query
sites
5minB

K

K

T

V

V

I

A

L

S

S

G

N

L

L

S

N

H

K

P

P

W

H

G

A

L

L

A

L

F

W

L

G

P

P

D

D

G

N

R

Q

M

I

L

W

V

L

T

T

E

E

K

R

-

A

D

T

G

G

R

K

L

I

R

L

I

R

V

V

A

N

P

P

D

E

G

S

T

G

V

S

S

V

A

K

P

T

V

V

K

F

G

Q

V

V

P

P

K

E

V

I

-

V

-

N

D

D

D

A

R

D

G

G

Q

Q

G

N

G

G

L

L

D

G

V

F

A

A

L

F

D

H

P

P

E

D

F

F

A

K

Q

N

N

N

R

P

F

Y

V

I

Y

Y

L

I

S

S

-

G

-

T

F

F

S

K

E

N

P

P

G

K

T

S

E

T

D

D

G

K

T

A

-

L

-

P

N

N

S

Q

T

T

A

I

V

I

A

R

R

R

G

Y

V

T

L

Y

N

N

A

K

D

S

E

T

T

D

A

T

L

L

T

E

D

K

V

P

R

V

V

D

I

L

F

L

S

A

Q

G

K

L

P

P

K

-

V

-

E

S

S

S

R

K

M

D

H
|
H

Y

Q

G

S

S

G

R

R

L

L

V

V

F

I

D

G

R

P

Q

D

G

Q

H

K

L

I

Y

Y

V

Y

T

T

L

I

G

G

E
x
D

R

Q

S

G

L

R

E

N

Q

Q

F

L

R

A

-

Y

-

L

-

F

-

L

-

P

T

N

Q

Q

A

A

Q

Q

-

H

-

T

-

P

-

T

-

Q

-

E

-

L

-

N

-

G

-

K

D

D

L

Y

D

H

S

T

H

Y

L

M

G

G

K

K

V

V

V

L

R

R

I

L

N

N

R

L

D

D

G

G

S

S

V

I

P

P

A

K

D

D

N

N

P

P

F

S

V

F

N

N

Q

-

S

-

G

G

A

V

L

V

P

S

E

H

I

I

W

Y

S

T

Y

L

G

G

H

H

R
|
R

N

N

I

P

Q

Q

G

G

A

L

A

A

L

F

N

T

P

P

Q

N

T

-

G

G

A

K

L

L

W

L

I

Q

N

S

E

E

H

Q

G

G

P

P

R

N

G

S

G

D

D

D

E
|
E

V

I

N

N

V

L

P

I

E

V

P

K

G

G

K

G

N

N

Y
|
Y

G

G

W

W

P

P

V

N

V

V

S
x
A

-

G

-
x
Y

-

K

-
x
D

-

D

-

S

-

G

-

Y

-

A

-

Y

-

A

-

N

-

Y

-

S

-

A

-

N

-

K

-

S

-

I

Y

K

G

D

V

L

N

A

Y

Q

N

N

G

G

T

V

P

K

I

V

G

A

-

A

-

G

-

V

-

P

-

V

-

T

-

K

-
x
E

-

S

-

E

-

W

T

T

G

G

K

K

S

N

S

F

A

V

P

P

G

P

M

L

E

K

-

T

-

L

-

Y

-

T

-

V

-

Q

-

D

-

T

-

Y

-

N

-

Y

-

N

-

D

-

P

-

T

-

C

-

G

E

E

P

M

V

T

Y

Y

Q

I

W

C

T

W

P

P

V

T

I

V

G

A

S

P

S

S

G

S

M

A

T

Y

F

V

Y

Y

T

K

A

G

-

G

-

K

D

K

T

A

L

I

P

T

G

G

W

W

K

E

G

N

S

T

L

L

F

L

N

V

G

P

G

S

L

L

A

K

T

R

K

G

E

V

V

I

V

F

R

R

L

I

E

K

L

L

D

D

-

P

-

T

-

Y

G

S

N

T

K

T

V

Y

K

D

H

D

E

A

E

V

R

P

M

M

F

F

R

K

D

S

L

-

G

N

K

N

R

R

I

Y

R

R

Q

D

V

V

S

I

Q

A

G

S

P

P

D

D

G

G

-

N

A

V

L

L

Y

Y

L

V

L

L

T

T

D

D

active site: H144 (= H158), D163 (≠ E177), R228 (= R228), A269 (≠ S270), Y271 (vs. gap), D273 (vs. gap), E309 (vs. gap)
binding calcium ion: E253 (= E254), Y263 (= Y264), A269 (≠ S270), Y271 (vs. gap), D273 (vs. gap), E309 (vs. gap)

1cq1A Soluble quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus in complex with pqqh2 and glucose (see paper)
29% identity, 86% coverage: 39:371/386 of query aligns to 20:418/444 of 1cq1A

query
sites
1cq1A

K

K

T

V

V

I

A

L

S

S

G

N

L

L

S

N

H

K

P

P

W

H

G

A

L

L

A

L

F

W

L

G

P

P

D

D

G

N

R

Q

M

I

L

W

V

L

T

T

E

E

K

R

-

A

D

T

G

G

R

K

L

I

R

L

I

R

V

V

A

N

P

P

D

E

G

S

T

G

V

S

S

V

A

K

P

T

V

V

K

F

G

Q

V

V

P

P

K

E

V

I

-

V

-

N

D

D

D

A

R

D

G

G

Q
|
Q

G

N

G

G

L

L

D

G

V

F

A

A

L

F

D

H

P

P

E

D

F

F

A

K

Q

N

N

N

R

P

F

Y

V

I

Y

Y

L

I

S

S

-

G

-

T

F

F

S

K

E

N

P

P

G

K

T

P

E

-

D

-

G

-

T

-

N

N

S

Q

T

T

A

I

V

I

A

R

R

R

G

Y

V

T

L

Y

N

N

A

K

D

S

E

T

T

D

A

T

L

L

T

E

D

K

V

P

R

V

V

D

I

L

F

L

S

A

Q

G

K

L

P

P

K

-

V

-

E

S

S

S

R

K

M
x
D

H
|
H

Y

Q

G

S

S

G

R

R

L

L

V

V

F

I

D

G

R

P

Q

D

G

Q

H

K

L

I

Y

Y

V

Y

T

T

L

I

G

G

E
x
D

R

Q

S

G

L

R

E

N

Q
|
Q

F

L

R

A

-

Y

-

L

-

F

-

L

-

P

T

N

Q

Q

A

A

Q

Q

-

H

-

T

-

P

-

T

-

Q

-

E

-

L

-

N

-

G

-

K

D

D

L

Y

D

H

S

T

H

Y

L

M

G

G

K

K

V

V

V

L

R

R

I

L

N

N

R

L

D

D

G

G

S

S

V

I

P

P

A

K

D

D

N

N

P

P

F

S

V

F

N

N

Q

-

S

-

G

G

A

V

L

V

P

S

E

H

I

I

W

Y

S

T

Y

L

G

G

H

H

R
|
R

N
|
N

I

P

Q

Q

G

G

A

L

A

A

L

F

N

T

P

P

Q

N

T

-

G

G

A

K

L

L

W

L

I

Q

N

S

E

E

H
x
Q

G
|
G

P
|
P

R

N

G

S

G

D

D

D

E
|
E

V

I

N

N

V

L

P

I

E

V

P

K

G

G

K

G

N

N

Y
|
Y

G

G

W

W

P

P

V

N

V

V

S
x
A

-

G

-
x
Y

-

K

-
x
D

-

D

-

S

-

G

-

Y

-

A

-

Y

-

A

-

N

-

Y

-

S

-

A

-

N

-

K

-

S

-

I

Y

K

G

D

V

L

N

A

Y

Q

N

N

G

G

T

V

P

K

I

V

G

A

-

A

-

G

-

V

-

P

-

V

-

T

-

K

-
x
E

-

S

-

E

-

W

T

T

G

G

K

K

S

N

S

F

A

V

P

P

G

P

M

L

E

K

-

T

-

L

-

Y

-

T

-

V

-

Q

-

D

-

T

-

Y

-

N

-

Y

-

N

-

D

-

P

-

T

-

C

-

G

E

E

P

M

V

T

Y
|
Y

Q

I

W

C

T

W

P

P

V
x
T

I

V

G
x
A

S

P

S

S

G

S

M

A

T

Y

F

V

Y

Y

T

K

A

G

-

G

-

K

D

K

T

A

L

I

P

T

G

G

W

W

K

E

G

N

S

T

L

L

F

L

N

V

G

P

G

S

L
|
L

A
x
K

T

R

K

G

E

V

V

I

V

F

R

R

L

I

E

K

L

L

D

D

-

P

-

T

-

Y

G

S

N

T

K

T

V

Y

K

D

H

D

E

A

E

V

R

P

M

M

F

F

R

K

D

S

L

-

G

N

K

N

R
|
R

I

Y

R
|
R

Q

D

V

V

S

I

Q

A

G

S

P

P

D

D

G

G

-

N

A

V

L

L

Y

Y

L

V

L

L

T

T

D

D

active site: H138 (= H158), D157 (≠ E177), R222 (= R228), A263 (≠ S270), Y265 (vs. gap), D267 (vs. gap), E303 (vs. gap)
binding beta-D-glucopyranose: D137 (≠ M157), H138 (= H158), Q162 (= Q182), Y337 (= Y295)
binding calcium ion: G241 (= G248), P242 (= P249), E247 (= E254), Y257 (= Y264), A263 (≠ S270), Y265 (vs. gap), D267 (vs. gap), E303 (vs. gap)
binding pyrroloquinoline quinone: Q76 (= Q92), H138 (= H158), R222 (= R228), N223 (= N229), Q240 (≠ H247), Y337 (= Y295), T342 (≠ V300), A344 (≠ G302), L370 (= L326), K371 (≠ A327), R400 (= R354), R402 (= R356)

1c9uA Crystal structure of the soluble quinoprotein glucose dehydrogenase in complex with pqq (see paper)
29% identity, 86% coverage: 39:371/386 of query aligns to 20:418/444 of 1c9uA

query
sites
1c9uA

K

K

T

V

V

I

A

L

S

S

G

N

L

L

S

N

H

K

P

P

W

H

G

A

L

L

A

L

F

W

L

G

P

P

D

D

G

N

R

Q

M

I

L

W

V

L

T

T

E

E

K

R

-

A

D

T

G

G

R

K

L

I

R

L

I

R

V

V

A

N

P

P

D

E

G

S

T

G

V

S

S

V

A

K

P

T

V

V

K

F

G

Q

V

V

P

P

K

E

V

I

-

V

-

N

D

D

D

A

R

D

G

G

Q
|
Q

G

N

G

G

L

L

D

G

V

F

A

A

L

F

D

H

P

P

E

D

F

F

A

K

Q

N

N

N

R

P

F

Y

V

I

Y

Y

L

I

S

S

-

G

-

T

F

F

S

K

E

N

P

P

G

K

T

P

E

-

D

-

G

-

T

-

N

N

S

Q

T

T

A

I

V

I

A

R

R

R

G

Y

V

T

L

Y

N

N

A

K

D

S

E

T

T

D

A

T

L

L

T

E

D

K

V

P

R

V

V

D

I

L

F

L

S

A

Q

G

K

L

P

P

K

-

V

-

E

S

S

S

R

K

M

D

H
|
H

Y

Q

G

S

S

G

R

R

L

L

V

V

F

I

D

G

R

P

Q

D

G

Q

H

K

L

I

Y

Y

V

Y

T

T

L

I

G

G

E
x
D

R

Q

S

G

L

R

E

N

Q

Q

F

L

R

A

-

Y

-

L

-

F

-

L

-

P

T

N

Q

Q

A

A

Q

Q

-

H

-

T

-

P

-

T

-

Q

-

E

-

L

-

N

-

G

-

K

D

D

L

Y

D

H

S

T

H

Y

L

M

G

G

K

K

V

V

V

L

R

R

I

L

N

N

R

L

D

D

G

G

S

S

V

I

P

P

A

K

D

D

N

N

P

P

F

S

V

F

N

N

Q

-

S

-

G

G

A

V

L

V

P

S

E

H

I

I

W

Y

S

T

Y

L

G

G

H

H

R
|
R

N
|
N

I

P

Q

Q

G

G

A

L

A

A

L

F

N

T

P

P

Q

N

T

-

G

G

A

K

L

L

W

L

I

Q

N

S

E

E

H
x
Q

G
|
G

P
|
P

R

N

G

S

G

D

D

D

E
|
E

V

I

N

N

V

L

P

I

E

V

P

K

G

G

K

G

N

N

Y
|
Y

G

G

W

W

P

P

V

N

V

V

S
x
A

-

G

-
x
Y

-

K

-
x
D

-

D

-

S

-

G

-

Y

-

A

-

Y

-

A

-

N

-

Y

-

S

-

A

-

N

-

K

-

S

-

I

Y

K

G

D

V

L

N

A

Y

Q

N

N

G

G

T

V

P

K

I

V

G

A

-

A

-

G

-

V

-

P

-

V

-

T

-

K

-
x
E

-

S

-

E

-

W

T

T

G

G

K

K

S

N

S

F

A

V

P

P

G

P

M

L

E

K

-

T

-

L

-

Y

-

T

-

V

-

Q

-

D

-

T

-

Y

-

N

-

Y

-

N

-

D

-

P

-

T

-

C

-

G

E

E

P

M

V

T

Y
|
Y

Q

I

W

C

T
x
W

P

P

V
x
T

I

V

G

A

S

P

S

S

G

S

M

A

T

Y

F

V

Y

Y

T

K

A

G

-

G

-

K

D

K

T

A

L

I

P

T

G

G

W

W

K

E

G

N

S

T

L

L

F

L

N

V

G

P

G

S

L
|
L

A
x
K

T

R

K

G

E

V

V

I

V

F

R

R

L

I

E

K

L

L

D

D

-

P

-

T

-

Y

G

S

N

T

K

T

V

Y

K

D

H

D

E

A

E

V

R

P

M

M

F

F

R

K

D

S

L

-

G

N

K

N

R
|
R

I

Y

R
|
R

Q

D

V

V

S

I

Q

A

G

S

P

P

D

D

G

G

-

N

A

V

L

L

Y

Y

L

V

L

L

T

T

D

D

active site: H138 (= H158), D157 (≠ E177), R222 (= R228), A263 (≠ S270), Y265 (vs. gap), D267 (vs. gap), E303 (vs. gap)
binding calcium ion: R222 (= R228), G241 (= G248), P242 (= P249), E247 (= E254), Y257 (= Y264), A263 (≠ S270), Y265 (vs. gap), D267 (vs. gap), E303 (vs. gap)
binding pyrroloquinoline quinone: Q76 (= Q92), H138 (= H158), R222 (= R228), N223 (= N229), Q240 (≠ H247), Y337 (= Y295), W340 (≠ T298), T342 (≠ V300), L370 (= L326), K371 (≠ A327), R400 (= R354), R402 (= R356)

3dasA Structure of the pqq-bound form of aldose sugar dehydrogenase (adh) from streptomyces coelicolor
30% identity, 88% coverage: 38:375/386 of query aligns to 11:317/334 of 3dasA

query
sites
3dasA

V

L

K

R

T

T

V

V

A

A

S

T

G

G

L

L

S

N

H

S

P

P

W

W

G

G

L

L

A

A

F

P

L

L

P

P

D

G

G

G

R

D

M

L

L

L

V

V

T

S

E

S

K

R

D

D

G

E

R

A

-

T

-

I

L

T

R

R

I

V

V

D

A

A

P

K

D

T

G

G

T

R

V

K

S

T

A

E

P

-

V

L

K

G

G

E

V

V

P

P

K

G

V

V

D

S

D

P

R

S

G

G

Q
x
E

G

G

L

L

D

G

V

I

A

A

L

L

D

S

P

P

E

D

F

Y

A

A

Q

S

N

D

R

H

F

M

V

V

Y

Y

L

A

S

Y

F

F

S

T

E

S

P

-

G

-

T

-

E

-

D

-

G

-

T

A

N

S

S

D

T

N

A

R

V

I

A

V

R

R

G

M

V

L

L

Y

N

D

A

E

D

K

E

K

T

P

A

S

-

G

-

E

-

Q

L

L

T

G

D

A

V

P

R

D

V

T

I

V

F

F

S

R

Q

G

K

I

P

P

K

K

V

-

E

-

S

G

R

V

M

I

H
|
H

Y

N

G

G

S

G

R

R

L

I

V

A

F

F

D

G

R

P

Q

D

G

K

H

M

L

L

Y

Y

V

A

T

G

L

T

G

G

E
|
E

R

S

S

G

L

D

E

T

Q

G

F

L

R

-

T

-

Q

-

A

S

Q

Q

D

D

L

R

D

K

S

S

H

L

L

G

G

G

K

K

V

I

V

L

R

R

I

M

N

T

R

P

D

D

G

G

S

E

V

P

P

A

A

P

D

G

N

N

P

P

F

F

V

P

N

G

Q

S

S

P

G

-

A

-

L

-

P

-

E

-

I

V

W

Y

S

S

Y

Y

G

G

H

H

R
|
R

N
|
N

I

V

Q
|
Q

G

G

A

L

A

A

L

W

N

D

P

D

Q
x
K

T

Q

G

-

A

R

L

L

W

F

I

A

N

S

E

E

H
x
F

G
|
G

P
x
Q

R

D

G

T

G

W

D

D

E
|
E

V

L

N

N

V

A

P

I

E

K

P

P

G

G

K

D

N

N

Y
|
Y

G

G

W

W

P

P

V

E

V

A

S
x
E

Y

-

G

-

V

-

N

-

Y

-

N

-

G

-

T

-

P

-

I

-

G

-

T

-

G

G

K
|
K

S

G

S
x
G

A

G

P

S

G

G

M

F

E

H

E

D

P

P

V

V

Y

A

Q

Q

W

W

T

S

P

T

V
x
D

I

E

G

A

S
|
S

S

P

G

S

M

G

T

I

F

A

Y

Y

T

A

A

-

D

-

T

-

L

-

P

-

G

-

W

-

K

E

G

G

S

S

L

V

F

W

N

M

G

A

G

G

L

L

A
x
R

T
x
G

K
x
E

E

R

V

L

V

W

R

R

L

I

E

P

L

L

D
x
K

G
|
G

N

T

K

A

V

A

K

A

H

A

E

D

E

P

R

Q

M

A

F

F

R

L

D

E

L

-

G

G

K

E

-

Y

-

G

R
|
R

I

L

R
|
R

Q

T

V

V

S

A

Q

P

G

A

P

G

D

G

G

D

A

K

L

L

Y

W

L

L

L

V

T

T

D

S

E

N

N

T

R

D

G

G

active site: H127 (= H158), E146 (= E177), R189 (= R228), E230 (≠ S270), K232 (= K284), G234 (≠ S286)
binding alpha-L-arabinopyranose: K199 (≠ Q238), D248 (≠ V300), R268 (≠ A327), G269 (≠ T328), E270 (≠ K329), K278 (≠ D337), G279 (= G338)
binding calcium ion: G208 (= G248), Q209 (≠ P249), E214 (= E254), Y224 (= Y264)
binding pyrroloquinoline quinone: E66 (≠ Q92), H127 (= H158), R189 (= R228), N190 (= N229), Q192 (= Q231), F207 (≠ H247), S251 (= S303), R268 (≠ A327), R296 (= R354), R298 (= R356)

Sites not aligning to the query:

binding pyrroloquinoline quinone: 318

2wfxB Crystal structure of the complex between human hedgehog-interacting protein hip and sonic hedgehog in the presence of calcium (see paper)
27% identity, 55% coverage: 35:245/386 of query aligns to 2:222/417 of 2wfxB

query
sites
2wfxB

M

C

V

F

S

C

V

I

K

Q

T

E

V

V

A

V

S

S

G

G

L

L

S

R

H

Q

P

P

W

V

G

G

L

A

A

L

F

H

L

S

P

G

D

D

G

G

-

S

-

Q

R

R

M

L

L

F

V

I

T

L

E

E

K

K

D

E

G

G

R

Y

L

V

R

K

I

I

V

L

A

T

P

P

D

E

G

G

T

E

V

I

-

F

S

K

A

E

P

P

V

Y

K

L

G

D

V

I

P

H

K

K

V

L

D

V

D

Q

R

S

G

G

-

I

-

K

-

D

Q

E

G

R

G

G

L

L

D

S

V

L

A

A

L

F

D

H

P

P

E

N

F

Y

A

K

Q

K

N

N

R

G

F

K

V

L

Y

Y

L

V

S

S

F

Y

S

T

E

T

P

N

G

D

T

H

E

I

D

L

G

R

T

V

N

V

S

E

T

Y

A

T

V

V

A

S

R

R

G

K

V

-

L

-

N

N

A

P

D

H

E

Q

T

V

A

D

L

L

T

R

D

T

V

A

R

R

V

V

I

-

F

F

S

L

Q

E

K

V

P

A

K

E

V

L

E

H

S

-

R

R

M

K

H

H

Y

L

G

G

S

G

R

Q

L

L

V

L

F

F

D

G

R

P

Q

D

G

G

H

F

L

L

Y

Y

V

I

T

I

L

L

G

G

E

D

R

G

S

M

L

I

E

T

Q

L

F

D

R

D

T

M

Q

E

A

E

Q

M

D
|
D

-

G

L

L

D

S

S

D

H

F

L

T

G

G

K

S

V

V

V

L

R

R

I

L

N

D

R

V

D

D

G

T

-

D

-

M

-

C

-

N

-

V

-

P

-

Y

S

S

V

I

P

P

A

R

D

S

N

N

P

P

F

H

V

F

N

N

Q

S

S

T

G

N

A

Q

L

P

P

P

E

E

I

V

W

F

S

A

Y

H

G

G

H

L

R

H

N

D

I

P

Q

G

G

R

A

C

A

A

L

V

N

D

P

R

Q

H

T

P

G

T

A

D

L

I

W

N

I

I

N

N

binding zinc ion: D158 (= D189)

Sites not aligning to the query:

binding zinc ion: 417

7pgnB Hhp-c in complex with glycosaminoglycan mimic sos (see paper)
26% identity, 50% coverage: 35:226/386 of query aligns to 3:208/438 of 7pgnB

query
sites
7pgnB

M

C

V

F

S

C

V

I

K

Q

T

E

V

V

A

V

S

S

G

G

L

L

S

R

H

Q

P

P

W

V

G

G

L

A

A

L

F

H

L

S

P

G

D

D

G

G

-

S

-

Q

R

R

M

L

L

F

V

I

T

L

E

E

K

K

D

E

G

G

R

Y

L

V

R

K

I

I

V

L

A

T

P

P

D

E

G

G

T

E

V

I

-

F

S

K

A

E

P

P

V

Y

K

L

G

D

V

I

P

H

K

K

V

L

D

V

D

Q

R

S

G

G

-

I

-
x
K

-
x
G

-

G

-

D

Q
x
E

G

R

G

G

L

L

D

S

V

L

A

A

L

F

D

H

P

P

E

N

F

Y

A

K

Q

K

N

N

R

G

F

K

V

L

Y

Y

L

V

S

S

F

Y

S

T

E

-

P

-

G

-

T

T

E

N

D

Q

G

H

T

D

N

H

S

I

T

L

A

R

V

V

A

V

R

E

G

Y

V

T

L

V

N

S

A

R

D

K

E

N

T

P

A

H

L

Q

T

V

D

D

V

L

R

R

V

T

-

A

-

R

I

V

F

F

S

L

Q

E

K

V

P

A

K

E

V

L

E

H

S

-

R
|
R

M
x
K

H

H

Y

L

G

G

S

G

R

Q

L

L

V

L

F

F

D

G

R

P

Q

D

G

G

H

F

L

L

Y

Y

V

I

T

I

L

L

G

G

E

D

R

G

S

M

L

I

E

T

Q

L

F

D

R

D

T

M

Q

E

A

E

Q

M

D

D

-

G

L

L

D

S

S

D

H

F

L

T

G

G

K

S

V

V

V

L

R

R

I

L

N

D

R

V

D

D

G

T

-

D

-

M

-

C

-

N

-

V

-

P

-

Y

S

S

V

I

P

P

A

R

D

S

N

N

P

P

F

H

V

F

N

N

Q

S

S

T

G

N

A

Q

L

P

P

P

E

E

I

V

W

F

S

A

Y

H

G

G

binding calcium ion: E68 (≠ Q92)
binding 2,3,4,6-tetra-O-sulfonato-alpha-D-glucopyranose: K64 (vs. gap), K64 (vs. gap), G65 (vs. gap), R130 (= R156), K131 (≠ M157)

Sites not aligning to the query:

7pgnA Hhp-c in complex with glycosaminoglycan mimic sos (see paper)
26% identity, 50% coverage: 35:226/386 of query aligns to 4:209/437 of 7pgnA

query
sites
7pgnA

M

C

V

F

S

C

V

I

K

Q

T

E

V

V

A

V

S

S

G

G

L

L

S

R

H

Q

P

P

W

V

G

G

L

A

A

L

F

H

L

S

P

G

D

D

G

G

-

S

-

Q

R

R

M

L

L

F

V

I

T

L

E

E

K

K

D

E

G

G

R

Y

L

V

R

K

I

I

V

L

A

T

P

P

D

E

G

G

T

E

V

I

-

F

S

K

A

E

P

P

V

Y

K

L

G

D

V

I

P

H

K

K

V

L

D

V

D

Q

R

S

G

G

-

I

-
x
K

-
x
G

-

D

Q

E

G

R

G

G

L

L

D

S

V

L

A

A

L

F

D

H

P

P

E

N

F

Y

A

K

Q

K

N

N

R

G

F

K

V

L

Y

Y

L

V

S

S

F

Y

S

T

E

-

P

-

G

-

T

T

E

N

D

Q

G

H

T

D

N

H

S

I

T

L

A

R

V

V

A

V

R

E

G

Y

V

T

L

V

N

S

A

R

D

K

E

N

T

P

A

H

L

Q

T

V

D

D

V

L

R

R

V

T

-

A

-

R

I

V

F

F

S

L

Q

E

K

V

P

A

K

E

V

L

E

H

S

-

R

R

M
x
K

H

H

Y

L

G

G

S

G

R

Q

L

L

V

L

F

F

D

G

R

P

Q

D

G

G

H

F

L

L

Y

Y

V

I

T

I

L

L

G

G

E

D

R

G

S

M

L

I

E

T

Q

L

F

D

R

D

T

M

Q

E

A

E

Q

M

D

D

-

G

L

L

D

S

S

D

H

F

L

T

G

G

K

S

V

V

V

L

R

R

I

L

N

D

R

V

D

D

G

T

-

D

-

M

-

C

-

N

-

V

-

P

-

Y

S

S

V

I

P

P

A

R

D

S

N

N

P

P

F

H

V

F

N

N

Q

S

S

T

G

N

A

Q

L

P

P

P

E

E

I

V

W

F

S

A

Y

H

G

G

binding 1,3,4,6-tetra-O-sulfo-beta-D-fructofuranose: K65 (vs. gap), G66 (vs. gap), G67 (vs. gap), K132 (≠ M157)

Sites not aligning to the query:

binding 2,3,4,6-tetra-O-sulfonato-alpha-D-glucopyranose: 283, 338

7pgmB Hhip-c in complex with heparin (see paper)
26% identity, 50% coverage: 35:226/386 of query aligns to 3:203/427 of 7pgmB

query
sites
7pgmB

M

C

V

F

S

C

V

I

K

Q

T

E

V

V

A

V

S

S

G

G

L

L

S

R

H

Q

P

P

W

V

G

G

L

A

A

L

F

H

L

S

P

G

D

D

G

G

-

S

-

Q

R

R

M

L

L

F

V

I

T

L

E

E

K

K

D

E

G

G

R

Y

L

V

R

K

I

I

V

L

A

T

P

P

D

E

G

G

T

E

V

I

-

F

S

K

A

E

P

P

V

Y

K

L

G

D

V

I

P

H

K

K

V

L

D

V

D

Q

R

S

G

G

-

I

-
x
K

-
x
G

-

G

-

D

Q

E

G

R

G

G

L

L

D

S

V

L

A

A

L

F

D

H

P

P

E

N

F

Y

A

K

Q

K

N

N

R

G

F

K

V

L

Y

Y

L

V

S

S

F

Y

S

T

E

-

P

-

G

-

T

T

E

N

D

Q

G

H

T

D

N

H

S

I

T

L

A

R

V

V

A

V

R

E

G

Y

V

T

L

V

N

S

A

R

D

K

E

N

T

P

A

H

L

Q

T

V

D

D

V

L

R

R

V

T

-

A

-

R

I

V

F

F

S

L

Q

E

K

V

P

A

K

E

V

L

E

H

S

-

R

R

M
x
K

H

H

Y

L

G

G

S

G

R

Q

L

L

V

L

F

F

D

G

R

P

Q

D

G

G

H

F

L

L

Y

Y

V

I

T

I

L

L

G

G

E

D

R

G

S

M

L

I

E

-

Q

-

F

-

R

-

T

-

Q

T

A

L

Q

D

D

D

L

M

D

E

S

S

H

D

L

F

-

T

G

G

K

S

V

V

V

L

R

R

I

L

N

D

R

V

D

D

G

T

-

D

-

M

-

C

-

N

-

V

-

P

-

Y

S

S

V

I

P

P

A

R

D

S

N

N

P

P

F

H

V

F

N

N

Q

S

S

T

G

N

A

Q

L

P

P

P

E

E

I

V

W

F

S

A

Y

H

G

G

binding 2-O-sulfo-alpha-L-idopyranuronic acid: K64 (vs. gap), G65 (vs. gap), K131 (≠ M157)
binding 2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose: K64 (vs. gap), G65 (vs. gap)

Sites not aligning to the query:

binding 2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose: 326

Q96QV1 Hedgehog-interacting protein; HHIP; HIP from Homo sapiens (Human) (see 3 papers)
26% identity, 56% coverage: 30:245/386 of query aligns to 211:447/700 of Q96QV1

query
sites
Q96QV1

K

K

T

H

E

K

K

H

A

N

M

C

V

F

S

C

V

I

K

Q

T

E

V

V

A

V

S

S

G

G

L

L

S

R

H

Q

P

P

W

V

G

G

L

A

A

L

F

H

L

S

P

G

D

D

G

G

-

S

-

Q

R

R

M

L

L

F

V

I

T

L

E

E

K

K

D

E

G

G

R

Y

L

V

R

K

I

I

V

L

A

T

P

P

D

E

G

G

T

E

V

I

-

F

S

K

A

E

P

P

V

Y

K

L

G

D

V

I

P

H

K

K

V

L

D

V

D

Q

R

S

G

G

-

I

-

K

-

G

-

D

Q

E

G

R

G

G

L

L

D

S

V

L

A

A

L

F

D

H

P

P

E

N

F

Y

A

K

Q

K

N

N

R

G

F

K

V

L

Y

Y

L

V

S

S

F

Y

S

T

E

-

P

-

G

-

T

-

E

-

D

-

G

-

T

T

N

N

S

Q

T

E

A

R

V

W

A

A

R

I

G

G

-

P

-

H

-

D

-

H

V

I

L

L

N

R

A

V

D

V

E

E

T

Y

A

T

L

V

T

S

-

R

-

K

-

N

-

P

-

H

-

Q

-

V

D

D

V

L

R

R

V

T

-

A

-

R

I
x
V

F

F

S

L

Q

E

K

V

P

A

K

E

V

L

E

H

S

-

R

R

M

K

H

H

Y

L

G

G

S

G

R

Q

L

L

V

L

F

F

D

G

R

P

Q

D

G

G

H

F

L

L

Y

Y

V

I

T

I

L

L

G

G

E

D

R

G

S

M

L

I

E

T

Q

L

F

D

R

D

T

M

Q
x
E

A

E

Q
x
M

D
|
D

-

G

L

L

D

S

S
x
D

H

F

L

T

G

G

K

S

V

V

V

L

R

R

I

L

N

D

R

V

D

D

G

T

-

D

-

M

-

C

-

N

-

V

-

P

-

Y

S

S

V

I

P

P

A

R

D

S

N

N

P

P

F

H

V

F

N

N

Q

S

S

T

G

N

A

Q

L

P

P

P

E

E

I

V

W

F

S

A

Y

H

G

G

H

L

R

H

N

D

I

P

Q

G

G

R

A

C

A

A

L

V

N

D

P

R

Q

H

T

P

G

T

A

D

L

I

W

N

I

I

N
|
N

V341 (≠ I146) natural variant: V -> I
E380 (≠ Q186) mutation to A: Abolishes SHH binding.
M382 (≠ Q188) mutation to A: Abolishes SHH binding.
D383 (= D189) mutation D->A,R: Abolishes SHH binding.
D387 (≠ S192) mutation to A: Abolishes SHH binding.
N447 (= N245) modified: carbohydrate, N-linked (GlcNAc...) asparagine

Query Sequence

>AZOBR_RS31355 FitnessBrowser__azobra:AZOBR_RS31355
MGRTAALMTATTLAACLSVTDASAVDKVFKTEKAMVSVKTVASGLSHPWGLAFLPDGRML
VTEKDGRLRIVAPDGTVSAPVKGVPKVDDRGQGGLLDVALDPEFAQNRFVYLSFSEPGTE
DGTNSTAVARGVLNADETALTDVRVIFSQKPKVESRMHYGSRLVFDRQGHLYVTLGERSL
EQFRTQAQDLDSHLGKVVRINRDGSVPADNPFVNQSGALPEIWSYGHRNIQGAALNPQTG
ALWINEHGPRGGDEVNVPEPGKNYGWPVVSYGVNYNGTPIGTGKSSAPGMEEPVYQWTPV
IGSSGMTFYTADTLPGWKGSLFNGGLATKEVVRLELDGNKVKHEERMFRDLGKRIRQVSQ
GPDGALYLLTDENRGEILRVTPAGKP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory