SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing AZOBR_RS33425 FitnessBrowser__azobra:AZOBR_RS33425 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6n0uA Crystal structure of a glucose-1-phosphate thymidylyltransferase from burkholderia phymatum bound to 2'-deoxy-thymidine-b-l-rhamnose
69% identity, 100% coverage: 2:288/288 of query aligns to 5:291/295 of 6n0uA

query
sites
6n0uA

K

K

G

G

I

I

L
|
L

A
|
A

G
|
G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

L

I

T

T

Q

H

V

V

T

V

S

S

K

K

Q

Q

L

L

P

P

V

V

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

T

T

L

L

M

M

L

V

A

A

G

G

I

I

R

R

D

D

I

V

L

L

I

I

T

S

T

T

P

P

D

Q

D

D

Q

T

P

P

Q

R

F

F

R

E

R

A

L

M

L

L

G

G

D

D

G

G

S

S

Q

Q

W

W

G

G

I

M

A

N

L

I

S

Q

Y

Y

A

A

E

V

Q
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Q

P

P

K

S

P
|
P

E
x
D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

R

R

E

E

F

F

V

V

G

G

R

N

D

D

D

P

V

S

C

A

L

L

I

I

L
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L

G

G

D
|
D

N

N

I

I

F

F

F

Y

G

G

H

H

D

D

L

L

E

A

P

K

A

Q

L

L

L

E

R

R

A

A

A

N

G

A

R

R

A

T

E

D

G

G

A

A

T

T

V

V

F

F

G

A

Y

Y

H

H

V

V

R

H

D

D

P

P

E

E

R

R

Y
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Y

G
|
G

V

V

I

V

S

E

F

F

D

D

A

K

E

D

G

F

V

R

P

A

V

L

A

S

I

I

E

E

E
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E

K
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K

P

P

T

A

V

K

P

P

K

R

S

S

N

N

Y

Y

A

A

V
|
V

T
|
T

G
|
G

L

L

Y
|
Y

F

F

Y

Y

D

D

N

N

A

Q

V

V

L

C

D

D

I

I

A

A

T

D

I

I

R

K

P

P

S

S

A

A

R
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R

G

G

E
|
E

L

L

E

E

I
|
I

T

T

D

D

V

V

N

N

N

S

V

R

Y

Y

L

L

E

S

R

Q

R

K

R

T

L

L

A

D

V

V

E

E

R

I

L

M

G

G

R

R

G

G

Y

Y

A

A

W
|
W

F

L

D

D

T

T

G

G

T

T

H

H

E

D

S

S

L

L

L

I

Q

E

A

A

E

T

F

F

V

I

Q

A

T

T

I

L

E

Q

A

K

R

R

Q

Q

G

G

L

L

K

V

V

V

C

A

S

C

P

P

E

E

V

I

A

A

W

Y

R

R

K

R

G

K

F

W

I

I

D

N

G

A

E

E

Q

Q

L

L

A

L

R

A

I

L

A

A

R

R

P

P

L

L

A

S

K

K

S

N

G

A

Y

Y

G

G

N

Q

Y

Y

L

L

L

Q

N

N

L

L

T

T

D

D

binding 2'-deoxy-thymidine-beta-l-rhamnose: L9 (= L6), A10 (= A7), G11 (= G8), G12 (= G9), Q83 (= Q80), P86 (= P83), D87 (≠ E84), G88 (= G85), L89 (= L86), L109 (= L106), D111 (= D108), Y146 (= Y143), G147 (= G144), E162 (= E159), K163 (= K160), V173 (= V170), T174 (= T171), G175 (= G172), Y177 (= Y174), R195 (= R192), E197 (= E194), I200 (= I197), W224 (= W221)

P26393 Glucose-1-phosphate thymidylyltransferase; dTDP-glucose pyrophosphorylase; Ep; dTDP-glucose synthase; EC 2.7.7.24 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
66% identity, 99% coverage: 2:286/288 of query aligns to 5:289/292 of P26393

query
sites
P26393

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

L

V

T

T

Q

M

V

A

T

V

S

S

K

K

Q

Q

L

L

P

P

V

I

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

T

T

L

L

M

M

L

L

A

A

G

G

I

I

R

R

D

D

I

I

L

L

I

I

T

S

T

T

P

P

D

Q

D

D

Q

T

P

P

Q

R

F

F

R

Q

L

L

G

G

D

D

G

G

S

S

Q

Q

W

W

G

G

I

L

A

N

L

L

S

Q

Y

Y

A

K

E

V

Q

Q

P

P

K

S

P

P

E

D

G

G

L

L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

R

E

E

E

F

F

V

I

G

G

R

H

D

D

V

C

C

A

L

L

I

V

L

L

G

G

D

D

N

N

I

I

F

F

F

Y

G

G

H

H

D

D

L

L

E

P

P

K

A

L

L

M

L

E

R

A

A

A

A

V

G

N

R

K

A

E

E

S

G

G

A

A

T

T

V

V

F

F

G

A

Y

Y

H

H

V

V

R

N

D

D

P

P

E

E

R

R

Y

Y

G

G

V

V

I

V

S

E

F

F

D

D

A

Q

E

K

G

G

V

T

P

A

V

V

A

S

I

L

E

E

K

K

P

P

T

L

V

Q

P

P

K

K

S

S

N

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

A

S

V

V

L

V

D

E

I

M

A

A

A

K

T

N

I

L

R

K

P

P

S

S

A

A

R

R

G

G

E

E

L

L

E

E

I

I

T
|
T

D

D

V

I

N

N

N

R

V

I

Y

Y

L

M

E

E

R

Q

R

G

R

R

L

L

A

S

V

V

E

A

R

M

L

M

G

G

R

R

G

G

Y

Y

A

A

W
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W

F

L

D

D

T

T

G

G

T

T

H

H

E

Q

S

S

L

L

L

I

Q

E

A

A

A

S

E

N

F

F

V

I

Q

A

T

T

I

I

E

E

A

E

R

R

Q

Q

G

G

L

L

K

K

V

V

C

S

S

C

P

P

E

E

V

I

A

A

W

F

R

R

K

K

G

N

F

F

I

I

D

N

G

A

E

Q

Q

Q

L

V

A

I

R

E

I

L

A

A

R

G

P

P

L

L

A

S

K

K

S

N

G

D

Y

Y

G

G

N

K

Y

Y

L

L

N

K

L

M

L

V

T201 (= T198) mutation to A: Two-fold increase in the conversion of 2-acetamido-2-deoxy-alpha-D-glucopyranosyl phosphate.
W224 (= W221) mutation to H: Is able to convert both 6-acetamido-6-deoxy-alpha-D-glucopyranosyl phosphate and alpha-D-glucopyranuronic acid 1-(dihydrogen phosphate), which are not accepted by the wild-type.

1h5tA Thymidylyltransferase complexed with thymidylyldiphosphate-glucose (see paper)
67% identity, 100% coverage: 2:288/288 of query aligns to 4:290/290 of 1h5tA

query
sites
1h5tA

K

K

G

G

I

I

L
|
L

A
|
A

G
|
G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

L

V

T

T

Q

M

V

A

T

V

S

S

K

K

Q

Q

L

L

P

P

V

I

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

T

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

D

D

I

I

L

L

I

I

T

S

T

T

P

P

D

Q

D

D

Q

T

P

P

Q

R

F

F

R

Q

L

L

G

G

D

D

G

G

S

S

Q

Q

W

W

G

G

I

L

A

N

L

L

S

Q

Y

Y

A

K

E

V

Q
|
Q

P

P

K

S

P
|
P

E
x
D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

R

E

E

E

F

F

V

I

G

G

R

G

D

D

V

C

C

A

L

L

I

V

L
|
L

G

G

D
|
D

N

N

I

I

F

F

F
x
Y

G
|
G

H
|
H

D
|
D

L
|
L

E
x
P

P

K

A

L

L

M

L

E

R

A

A

A

A

V

G

N

R

K

A

E

E

S

G

G

A

A

T

T

V

V

F

F

G

A

Y

Y

H

H

V

V

R

N

D

D

P

P

E

E

R

R

Y
|
Y

G
|
G

V

V

I

V

S

E

F

F

D

D

A

K

E

N

G

G

V

T

P

A

V

I

A

S

I

L

E

E

E
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E

K
|
K

P

P

T

L

V

E

P

P

K

K

S

S

N

N

Y

Y

A

A

V
|
V

T

T

G

G

L

L

Y
|
Y

F

F

Y

Y

D

D

N

N

A

D

V

V

L

V

D

Q

I

M

A

A

A

K

T

N

I

L

R

K

P

P

S

S

A

A

R
|
R

G

G

E
|
E

L

L

E

E

I

I

T

T

D

D

V

I

N

N

N

R

V

I

Y

Y

L

L

E

E

R

Q

R

G

R

R

L

L

A

S

V

V

E

A

R

M

L

M

G
|
G

R
|
R

G
|
G

Y

Y

A

A

W
|
W

F

L

D

D

T

T

G

G

T

T

H

H

E

Q

S

S

L

L

L

I

Q

E

A

A

A

S

E

N

F

F

V

I

Q

A

T

T

I

I

E

E

A

E

R

R

Q

Q

G

G

L

L

K
|
K

V
|
V

C
x
S

S

C

P

P

E

E

E
|
E

V
x
I

A

A

W

F

R

R

K

K

G

G

F

F

I

I

D

D

G

V

E

E

Q

Q

L

V

A

R

R

K

I

L

A

A

R

V

P

P

L

L

A

I

K

K

S

N

G

N

Y

Y

G

G

N

Q

Y

Y

L

L

L

Y

N

K

L

M

L

T

T

K

D

D

binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: L8 (= L6), A9 (= A7), G10 (= G8), G11 (= G9), Q82 (= Q80), P85 (= P83), D86 (≠ E84), G87 (= G85), L88 (= L86), L108 (= L106), D110 (= D108), Y145 (= Y143), G146 (= G144), E161 (= E159), K162 (= K160), V172 (= V170), Y176 (= Y174), R194 (= R192), E196 (= E194), W223 (= W221)
binding thymidine-5'-diphosphate: L45 (= L43), Y114 (≠ F112), G115 (= G113), H116 (= H114), D117 (= D115), L118 (= L116), P119 (≠ E117), G218 (= G216), R219 (= R217), G220 (= G218), K249 (= K247), V250 (= V248), S251 (≠ C249), E255 (= E253), I256 (≠ V254)

1h5rA Thymidylyltransferase complexed with thimidine and glucose-1-phospate (see paper)
67% identity, 100% coverage: 2:288/288 of query aligns to 4:290/290 of 1h5rA

query
sites
1h5rA

K

K

G

G

I

I

L
|
L

A
|
A

G
|
G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

L

V

T

T

Q

M

V

A

T

V

S

S

K

K

Q

Q

L

L

P

P

V

I

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

T

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

D

D

I

I

L

L

I

I

T

S

T

T

P

P

D

Q

D

D

Q

T

P

P

Q

R

F

F

R

Q

L

L

G

G

D

D

G

G

S

S

Q

Q

W

W

G

G

I

L

A

N

L

L

S

Q

Y

Y

A

K

E

V

Q
|
Q

P

P

K

S

P
|
P

E
x
D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

R

E

E

E

F

F

V

I

G

G

R

G

D

D

V

C

C

A

L

L

I

V

L
|
L

G

G

D
|
D

N

N

I

I

F

F

F
x
Y

G
|
G

H

H

D

D

L
|
L

E

P

P

K

A

L

L

M

L

E

R

A

A

A

A

V

G

N

R

K

A

E

E

S

G

G

A

A

T

T

V

V

F

F

G

A

Y

Y

H

H

V

V

R

N

D

D

P

P

E

E

R

R

Y
|
Y

G
|
G

V

V

I

V

S

E

F

F

D

D

A

K

E

N

G

G

V

T

P

A

V

I

A

S

I

L

E

E

E
|
E

K
|
K

P

P

T

L

V

E

P

P

K

K

S

S

N

N

Y

Y

A

A

V
|
V

T
|
T

G
|
G

L

L

Y
|
Y

F

F

Y

Y

D

D

N

N

A

D

V

V

L

V

D

Q

I

M

A

A

A

K

T

N

I

L

R

K

P

P

S

S

A

A

R

R

G

G

E
|
E

L

L

E

E

I

I

T

T

D

D

V

I

N

N

N

R

V

I

Y

Y

L

L

E

E

R

Q

R

G

R

R

L

L

A

S

V

V

E

A

R

M

L

M

G

G

R

R

G

G

Y

Y

A

A

W
|
W

F

L

D

D

T

T

G

G

T

T

H

H

E

Q

S

S

L

L

L

I

Q

E

A

A

A

S

E

N

F

F

V

I

Q

A

T

T

I

I

E

E

A

E

R

R

Q

Q

G

G

L

L

K
|
K

V
|
V

C
x
S

S

C

P

P

E

E

E
|
E

V
x
I

A

A

W

F

R
|
R

K

K

G

G

F

F

I

I

D

D

G

V

E

E

Q

Q

L

V

A

R

R

K

I

L

A

A

R

V

P

P

L

L

A

I

K

K

S

N

G

N

Y

Y

G

G

N

Q

Y

Y

L

L

L

Y

N

K

L

M

L

T

T

K

D

D

binding 1-O-phosphono-alpha-D-glucopyranose: L88 (= L86), L108 (= L106), Y145 (= Y143), G146 (= G144), E161 (= E159), K162 (= K160), V172 (= V170), T173 (= T171), G174 (= G172), Y176 (= Y174), W223 (= W221)
binding thymidine: L8 (= L6), A9 (= A7), G10 (= G8), G11 (= G9), L45 (= L43), Q82 (= Q80), P85 (= P83), D86 (≠ E84), G87 (= G85), L88 (= L86), L108 (= L106), D110 (= D108), Y114 (≠ F112), G115 (= G113), L118 (= L116), E196 (= E194), K249 (= K247), V250 (= V248), S251 (≠ C249), E255 (= E253), I256 (≠ V254), R259 (= R257)

1iinA Thymidylyltransferase complexed with udp-glucose (see paper)
66% identity, 99% coverage: 2:286/288 of query aligns to 5:289/289 of 1iinA

query
sites
1iinA

K

K

G

G

I

I

L
|
L

A

A

G
|
G

S

S

G

G

T

T

R
|
R

L

L

Y

Y

P

P

L

V

T

T

Q

M

V

A

T

V

S

S

K

Q

Q

Q

L

L

P

P

V

I

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

T

T

L

L

M

M

L

L

A

A

G

G

I

I

R

R

D

D

I

I

L

L

I

I

T

S

T

T

P

P

D

Q

D

D

Q

T

P

P

Q

R

F

F

R

Q

L

L

G

G

D

D

G

G

S

S

Q

Q

W

W

G

G

I

L

A

N

L

L

S

Q

Y

Y

A

K

E

V

Q
|
Q

P

P

K

S

P
|
P

E
x
D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

R

E

E

E

F

F

V

I

G

G

R

H

D

D

V

C

C

A

L

L

I

V

L
|
L

G

G

D
|
D

N
|
N

I

I

F

F

F

Y

G

G

H

H

D

D

L

L

E

P

P

K

A

L

L

M

L

E

R

A

A

A

A

V

G

N

R

K

A

E

E

S

G

G

A

A

T

T

V

V

F

F

G

A

Y

Y

H

H

V

V

R

N

D

D

P

P

E

E

R

R

Y
|
Y

G
|
G

V

V

I

V

S

E

F

F

D

D

A

Q

E

K

G

G

V

T

P

A

V

V

A

S

I

L

E

E

E
|
E

K
|
K

P

P

T

L

V

Q

P

P

K

K

S

S

N

N

Y

Y

A

A

V
|
V

T

T

G

G

L

L

Y
|
Y

F

F

Y

Y

D

D

N

N

A

S

V

V

L

V

D

E

I

M

A

A

A

K

T

N

I

L

R

K

P

P

S

S

A

A

R
|
R

G

G

E

E

L

L

E

E

I
|
I

T

T

D

D

V

I

N

N

N

R

V

I

Y

Y

L

M

E

E

R

Q

R

G

R

R

L

L

A

S

V

V

E

A

R

M

L

M

G

G

R

R

G

G

Y

Y

A

A

W
|
W

F

L

D

D

T

T

G

G

T

T

H

H

E

Q

S

S

L

L

L

I

Q

E

A

A

A

S

E

N

F

F

V

I

Q

A

T

T

I

I

E

E

A

E

R

R

Q

Q

G

G

L

L

K

K

V

V

C

S

S

C

P

P

E

E

V

I

A

A

W

F

R

R

K

K

G

N

F

F

I

I

D

N

G

A

E

Q

Q

Q

L

V

A

I

R

E

I

L

A

A

R

G

P

P

L

L

A

S

K

K

S

N

G

D

Y

Y

G

G

N

K

Y

Y

L

L

N

K

L

M

L

V

binding uridine-5'-diphosphate-glucose: L9 (= L6), G11 (= G8), G12 (= G9), R16 (= R13), Q83 (= Q80), P86 (= P83), D87 (≠ E84), G88 (= G85), L89 (= L86), L109 (= L106), D111 (= D108), N112 (= N109), Y146 (= Y143), G147 (= G144), E162 (= E159), K163 (= K160), V173 (= V170), Y177 (= Y174), R195 (= R192), I200 (= I197), W224 (= W221)

1iimA Thymidylyltransferase complexed with ttp (see paper)
66% identity, 99% coverage: 2:286/288 of query aligns to 5:289/289 of 1iimA

query
sites
1iimA

K

K

G

G

I

I

L
|
L

A
|
A

G
|
G

S
|
S

G
|
G

T
|
T

R
|
R

L

L

Y

Y

P

P

L

V

T

T

Q

M

V

A

T

V

S

S

K
x
Q

Q
|
Q

L

L

P

P

V

I

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

T

T

L

L

M

M

L
|
L

A

A

G

G

I

I

R

R

D

D

I

I

L

L

I

I

T

S

T

T

P

P

D

Q

D

D

Q

T

P

P

Q

R

F

F

R

Q

L

L

G

G

D

D

G

G

S

S

Q

Q

W

W

G

G

I

L

A

N

L

L

S

Q

Y

Y

A

K

E

V

Q
|
Q

P

P

K

S

P
|
P

E
x
D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

R

E

E

E

F

F

V

I

G

G

R

H

D

D

V

C

C

A

L

L

I

V

L
|
L

G
|
G

D
|
D

N

N

I

I

F

F

F
x
Y

G
|
G

H
|
H

D
|
D

L
|
L

E
x
P

P

K

A

L

L

M

L

E

R

A

A

A

A

V

G

N

R

K

A

E

E

S

G

G

A

A

T

T

V

V

F

F

G

A

Y

Y

H

H

V

V

R

N

D

D

P

P

E

E

R

R

Y

Y

G

G

V

V

I

V

S

E

F

F

D

D

A

Q

E

K

G

G

V

T

P

A

V

V

A

S

I

L

E

E

K

K

P

P

T

L

V

Q

P

P

K

K

S

S

N

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

A

S

V

V

L

V

D

E

I

M

A

A

A

K

T

N

I

L

R

K

P

P

S

S

A

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

I

N

N

N

R

V

I

Y

Y

L

M

E

E

R

Q

R

G

R

R

L

L

A

S

V

V

E

A

R

M

L

M

G
|
G

R
|
R

G
|
G

Y

Y

A

A

W

W

F

L

D

D

T

T

G

G

T

T

H

H

E

Q

S

S

L

L

L

I

Q

E

A

A

A

S

E

N

F

F

V

I

Q

A

T

T

I

I

E

E

A

E

R

R

Q

Q

G

G

L

L

K

K

V
|
V

C
x
S

S

C

P

P

E

E

E
|
E

V
x
I

A

A

W

F

R
|
R

K

K

G

N

F

F

I

I

D

N

G

A

E

Q

Q

Q

L

V

A

I

R

E

I

L

A

A

R

G

P

P

L

L

A

S

K

K

S

N

G

D

Y

Y

G

G

N

K

Y

Y

L

L

N

K

L

M

L

V

binding thymidine-5'-triphosphate: L9 (= L6), A10 (= A7), G11 (= G8), G12 (= G9), S13 (= S10), G14 (= G11), T15 (= T12), R16 (= R13), Q26 (≠ K23), Q27 (= Q24), L46 (= L43), Q83 (= Q80), P86 (= P83), D87 (≠ E84), G88 (= G85), L89 (= L86), L109 (= L106), G110 (= G107), D111 (= D108), Y115 (≠ F112), G116 (= G113), H117 (= H114), D118 (= D115), L119 (= L116), P120 (≠ E117), G219 (= G216), R220 (= R217), G221 (= G218), V251 (= V248), S252 (≠ C249), E256 (= E253), I257 (≠ V254), R260 (= R257)

3pkpA Q83s variant of s. Enterica rmla with datp (see paper)
66% identity, 99% coverage: 2:286/288 of query aligns to 5:289/290 of 3pkpA

query
sites
3pkpA

K

K

G

G

I

I

L
|
L

A
|
A

G
|
G

S
|
S

G
|
G

T
|
T

R
|
R

L

L

Y

Y

P

P

L

V

T

T

Q

M

V

A

T

V

S

S

K
|
K

Q
|
Q

L

L

P

P

V

I

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

T

T

L

L

M

M

L

L

A

A

G

G

I

I

R

R

D

D

I

I

L

L

I

I

I
|
I

T

S

T

T

P

P

D

Q

D

D

Q

T

P

P

Q

R

F

F

R

Q

L

L

G

G

D

D

G

G

S

S

Q

Q

W

W

G

G

I

L

A

N

L

L

S

Q

Y

Y

A

K

E

V

Q
x
S

P

P

K
x
S

P
|
P

E

D

G

G

L
|
L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

R

E

E

E

F

F

V

I

G

G

R

H

D

D

V

C

C

A

L

L

I

V

L
|
L

G
|
G

D
|
D

N

N

I

I

F

F

F

Y

G

G

H

H

D

D

L

L

E

P

P

K

A

L

L

M

L

E

R

A

A

A

A

V

G

N

R

K

A

E

E

S

G

G

A

A

T

T

V

V

F

F

G

A

Y

Y

H

H

V

V

R

N

D

D

P

P

E

E

R

R

Y

Y

G

G

V

V

I

V

S

E

F

F

D

D

A

Q

E

K

G

G

V

T

P

A

V

V

A

S

I

L

E

E

K

K

P

P

T

L

V

Q

P

P

K

K

S

S

N

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

A

S

V

V

L

V

D

E

I

M

A

A

A

K

T

N

I

L

R

K

P

P

S

S

A

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

I

N

N

N

R

V

I

Y

Y

L

M

E

E

R

Q

R

G

R

R

L

L

A

S

V

V

E

A

R

M

L

M

G

G

R

R

G

G

Y

Y

A

A

W

W

F

L

D
|
D

T

T

G

G

T

T

H

H

E

Q

S

S

L

L

L

I

Q

E

A

A

A

S

E

N

F

F

V

I

Q

A

T

T

I

I

E

E

A

E

R

R

Q

Q

G

G

L

L

K

K

V

V

C

S

S

C

P

P

E

E

V

I

A

A

W

F

R

R

K

K

G

N

F

F

I

I

D

N

G

A

E

Q

Q

Q

L

V

A

I

R

E

I

L

A

A

R

G

P

P

L

L

A

S

K

K

S

N

G

D

Y

Y

G

G

N

K

Y

Y

L

L

N

K

L

M

L

V

binding 2'-deoxyadenosine 5'-triphosphate: L9 (= L6), A10 (= A7), G11 (= G8), G12 (= G9), S13 (= S10), G14 (= G11), T15 (= T12), R16 (= R13), K26 (= K23), Q27 (= Q24), I55 (= I52), S83 (≠ Q80), S85 (≠ K82), P86 (= P83), L89 (= L86), L109 (= L106), G110 (= G107), D111 (= D108)
binding magnesium ion: D111 (= D108), D226 (= D223)

1h5sB Thymidylyltransferase complexed with tmp (see paper)
67% identity, 100% coverage: 2:288/288 of query aligns to 5:291/291 of 1h5sB

query
sites
1h5sB

K

K

G

G

I

I

L
|
L

A

A

G
|
G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

L

V

T

T

Q

M

V

A

T

V

S

S

K

K

Q

Q

L

L

P

P

V

I

F

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

T

T

L

L

M

M

L

L

A

A

G

G

I

I

R

R

D

D

I

I

L

L

I

I

T

S

T

T

P

P

D

Q

D

D

Q

T

P

P

Q

R

F

F

R

Q

L

L

G

G

D

D

G

G

S

S

Q

Q

W

W

G

G

I

L

A

N

L

L

S

Q

Y

Y

A

K

E

V

Q
|
Q

P

P

K

S

P
|
P

E
x
D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

R

E

E

E

F

F

V

I

G

G

R

G

D

D

V

C

C

A

L

L

I

V

L

L

G

G

D
|
D

N

N

I

I

F

F

F
x
Y

G
|
G

H
|
H

D
|
D

L

L

E

P

P

K

A

L

L

M

L

E

R

A

A

A

A

V

G

N

R

K

A

E

E

S

G

G

A

A

T

T

V

V

F

F

G

A

Y

Y

H

H

V

V

R

N

D

D

P

P

E

E

R

R

Y

Y

G

G

V

V

I

V

S

E

F

F

D

D

A

K

E

N

G

G

V

T

P

A

V

I

A

S

I

L

E

E

K

K

P

P

T

L

V

E

P

P

K

K

S

S

N

N

Y

Y

A

A

V

V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

A

D

V

V

L

V

D

Q

I

M

A

A

A

K

T

N

I

L

R

K

P

P

S

S

A

A

R

R

G

G

E
|
E

L

L

E

E

I

I

T

T

D

D

V

I

N

N

N

R

V

I

Y

Y

L

L

E

E

R

Q

R

G

R

R

L

L

A

S

V

V

E

A

R

L

L

M

G
|
G

R
|
R

G
|
G

Y

Y

A

A

W

W

F

L

D

D

T

T

G

G

T

T

H

H

E

Q

S

S

L

L

L

I

Q

E

A

A

A

S

E

N

F

F

V

I

Q

A

T

T

I

I

E

E

A

E

R

R

Q

Q

G

G

L

L

K
|
K

V
|
V

C
x
S

S

C

P

P

E

E

E
|
E

V
x
I

A

A

W

F

R
|
R

K

K

G

G

F

F

I

I

D

D

G

V

E

E

Q

Q

L

V

A

R

R

K

I

L

A

A

R

V

P

P

L

L

A

I

K

K

S

N

G

N

Y

Y

G

G

N

Q

Y

Y

L

L

L

Y

N

K

L

Q

L

T

T

K

D

D

binding thymidine-5'-phosphate: L9 (= L6), G11 (= G8), G12 (= G9), Q83 (= Q80), P86 (= P83), D87 (≠ E84), G88 (= G85), L89 (= L86), D111 (= D108), Y115 (≠ F112), G116 (= G113), H117 (= H114), D118 (= D115), E197 (= E194), G219 (= G216), R220 (= R217), G221 (= G218), K250 (= K247), V251 (= V248), S252 (≠ C249), E256 (= E253), I257 (≠ V254), R260 (= R257)

4ho9A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with udp-galactose and utp
68% identity, 99% coverage: 1:285/288 of query aligns to 1:285/294 of 4ho9A

query
sites
4ho9A

M

M

K

K

G

G

I

I

L
|
L

A
x
S

G
|
G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

L

L

T

T

Q

K

V

V

T

V

S

S

K

K

Q

Q

L

L

P

P

V

V

F

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

L

L

S

S

T

V

L

L

M

M

L
|
L

A

A

G

G

I

I

R

K

D

D

I

I

L

L

I

I

T

S

T

T

P

P

D

E

D

D

Q

T

P

P

Q

R

F

F

R

E

R

Q

L

L

G

G

D

G

G

G

S

S

Q

E

W

L

G

G

I

I

A

S

L

L

S

S

Y

Y

A

A

E

V

Q
|
Q

P

S

K

S

P
|
P

E
x
D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

R

E

E

E

F

F

V

I

G

G

R

D

D

D

D

N

V

V

C

A

L

L

I

V

L
|
L

G

G

D
|
D

N
|
N

I

I

F

F

F
x
Y

G
|
G

H
|
H

D
x
G

L
x
F

E
x
T

P
x
E

A

L

L

L

L

Q

R

R

A

A

G

N

R

R

A

K

E

S

G

G

A

A

T

T

V

I

F

F

G

G

Y

Y

H

N

V

V

R

K

D

D

P

P

E

Q

R

R

Y

F

G
|
G

V

V

I

V

S

E

F

F

D

D

A

E

E

K

G

G

V

K

P

V

V

I

A

S

I

I

E

E

E
|
E

K
|
K

P

P

T

E

V

E

P

P

K

K

S

S

N

S

Y

Y

A

A

V
|
V

T
|
T

G
|
G

L

L

Y
|
Y

F

F

Y

Y

D

D

N

N

A

R

V

V

L

V

D

D

I

I

A

A

A

K

T

N

I

I

R

T

P

P

S

S

A

A

R
|
R

G

G

E

E

L

L

E

E

I
|
I

T

T

D

D

V

V

N

N

N

K

V

A

Y

Y

L

L

E

E

R

L

R

G

R

E

L

L

A

H

V

V

E

E

R

L

L

L

G
|
G

R
|
R

G
|
G

Y

F

A

A

W
|
W

F

L

D

D

T

T

G

G

T

T

H

H

E

E

S

S

L

L

Q

Q

A

A

A

S

E

Q

F

F

V

I

Q

E

T

T

I

I

E

E

A

K

R

R

Q

Q

G

S

L

L

K
|
K

V
|
V

C
x
A

S

C

P

L

E

E

E
|
E

V
x
I

A

A

W

Y

R
|
R

K

M

G

G

F

Y

I

I

D

S

G

R

E

E

Q

Q

L

L

A

I

R

K

I

L

A

A

R

E

P

P

L

L

A

M

K

K

S

N

G

E

Y

Y

G

G

N

Q

Y

Y

L

L

L

M

N

N

L

L

binding galactose-uridine-5'-diphosphate: L6 (= L6), S7 (≠ A7), G8 (= G8), G9 (= G9), Q80 (= Q80), P83 (= P83), D84 (≠ E84), G85 (= G85), L86 (= L86), L106 (= L106), D108 (= D108), N109 (= N109), G144 (= G144), E159 (= E159), K160 (= K160), V170 (= V170), T171 (= T171), G172 (= G172), Y174 (= Y174), R192 (= R192), I197 (= I197), W221 (= W221)
binding uridine 5'-triphosphate: L43 (= L43), Y112 (≠ F112), G113 (= G113), H114 (= H114), G115 (≠ D115), F116 (≠ L116), T117 (≠ E117), E118 (≠ P118), G216 (= G216), R217 (= R217), G218 (= G218), K247 (= K247), V248 (= V248), A249 (≠ C249), E253 (= E253), I254 (≠ V254), R257 (= R257)

4ho4A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with thymidine and glucose-1-phosphate
68% identity, 99% coverage: 1:285/288 of query aligns to 1:285/289 of 4ho4A

query
sites
4ho4A

M

M

K

K

G

G

I

I

L

L

A

S

G

G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

L

L

T

T

Q

K

V

V

T

V

S

S

K

K

Q

Q

L

L

P

P

V

V

F

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

L

L

S

S

T

V

L

L

M

M

L

L

A

A

G

G

I

I

R

K

D

D

I

I

L

L

I

I

T

S

T

T

P

P

D

E

D

D

Q

T

P

P

Q

R

F

F

R

E

R

Q

L

L

G

G

D

G

G

G

S

S

Q

E

W

L

G

G

I

I

A

S

L

L

S

S

Y

Y

A

A

E

V

Q

Q

P

S

K

S

P

P

E

D

G

G

L
|
L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

R

E

E

E

F

F

V

I

G

G

R

D

D

D

D

N

V

V

C

A

L

L

I

V

L
|
L

G

G

D

D

N
|
N

I

I

F

F

F

Y

G

G

H

H

D

G

L

F

E

T

P

E

A

L

L

L

L

Q

R

R

A

A

G

N

R

R

A

K

E

S

G

G

A

A

T

T

V

I

F

F

G

G

Y

Y

H

N

V

V

R

K

D

D

P

P

E

Q

R

R

Y
x
F

G
|
G

V

V

I

V

S

E

F

F

D

D

A

E

E

K

G

G

V

K

P

V

V

I

A

S

I

I

E

E

E
|
E

K
|
K

P

P

T

E

V

E

P

P

K

K

S

S

N

S

Y

Y

A

A

V
|
V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

A

R

V

V

L

V

D

D

I

I

A

A

A

K

T

N

I

I

R

T

P

P

S

S

A

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

V

N

N

N

K

V

A

Y

Y

L

L

E

E

R

L

R

G

R

E

L

L

A

H

V

V

E

E

R

L

L

L

G

G

R

R

G

G

Y

F

A

A

W
|
W

F

L

D

D

T

T

G

G

T

T

H

H

E

E

S

S

L

L

Q

Q

A

A

A

S

E

Q

F

F

V

I

Q

E

T

T

I

I

E

E

A

K

R

R

Q

Q

G

S

L

L

K

K

V

V

C

A

S

C

P

L

E

E

V

I

A

A

W

Y

R

R

K

M

G

G

F

Y

I

I

D

S

G

R

E

E

Q

Q

L

L

A

I

R

K

I

L

A

A

R

E

P

P

L

L

A

M

K

K

S

N

G

E

Y

Y

G

G

N

Q

Y

Y

L

L

L

M

N

N

L

L

binding 1-O-phosphono-alpha-D-glucopyranose: L86 (= L86), L106 (= L106), N109 (= N109), F143 (≠ Y143), G144 (= G144), E159 (= E159), K160 (= K160), V170 (= V170), W221 (= W221)

4ho6A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with udp-glucose and utp
68% identity, 99% coverage: 1:285/288 of query aligns to 1:285/288 of 4ho6A

query
sites
4ho6A

M

M

K

K

G

G

I

I

L
|
L

A
x
S

G
|
G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

L

L

T

T

Q

K

V

V

T

V

S

S

K

K

Q

Q

L

L

P

P

V

V

F

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

L

L

S

S

T

V

L

L

M

M

L
|
L

A

A

G

G

I

I

R

K

D

D

I

I

L

L

I

I

T

S

T

T

P

P

D

E

D

D

Q

T

P

P

Q

R

F

F

R

E

R

Q

L

L

G

G

D

G

G

G

S

S

Q

E

W

L

G

G

I

I

A

S

L

L

S

S

Y

Y

A

A

E

V

Q
|
Q

P

S

K

S

P
|
P

E
x
D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

R

E

E

E

F

F

V

I

G

G

R

D

D

D

D

N

V

V

C

A

L

L

I

V

L
|
L

G

G

D
|
D

N

N

I

I

F

F

F
x
Y

G
|
G

H
|
H

D
x
G

L
x
F

E
x
T

P

E

A

L

L

L

L

Q

R

R

A

A

G

N

R

R

A

K

E

S

G

G

A

A

T

T

V

I

F

F

G

G

Y

Y

H

N

V

V

R

K

D

D

P

P

E

Q

R

R

Y
x
F

G
|
G

V

V

I

V

S

E

F

F

D

D

A

E

E

K

G

G

V

K

P

V

V

I

A

S

I

I

E

E

E
|
E

K
|
K

P

P

T

E

V

E

P

P

K

K

S

S

N

S

Y

Y

A

A

V
|
V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

A

R

V

V

L

V

D

D

I

I

A

A

A

K

T

N

I

I

R

T

P

P

S

S

A

A

R
|
R

G

G

E

E

L

L

E

E

I
|
I

T

T

D

D

V

V

N

N

N

K

V

A

Y

Y

L

L

E

E

R

L

R

G

R

E

L

L

A

H

V

V

E

E

R

L

L

L

G

G

R

R

G

G

Y

F

A

A

W
|
W

F

L

D

D

T

T

G

G

T

T

H

H

E

E

S

S

L

L

Q

Q

A

A

A

S

E

Q

F

F

V

I

Q

E

T

T

I

I

E

E

A

K

R

R

Q

Q

G

S

L

L

K
|
K

V
|
V

C
x
A

S

C

P

L

E

E

E
|
E

V
x
I

A

A

W

Y

R
|
R

K

M

G

G

F

Y

I

I

D

S

G

R

E

E

Q

Q

L

L

A

I

R

K

I

L

A

A

R

E

P

P

L

L

A

M

K

K

S

N

G

E

Y

Y

G

G

N

Q

Y

Y

L

L

L

M

N

N

L

L

binding uridine-5'-diphosphate-glucose: L6 (= L6), S7 (≠ A7), G8 (= G8), G9 (= G9), Q80 (= Q80), P83 (= P83), D84 (≠ E84), G85 (= G85), L86 (= L86), L106 (= L106), D108 (= D108), F143 (≠ Y143), G144 (= G144), E159 (= E159), K160 (= K160), V170 (= V170), R192 (= R192), I197 (= I197), W221 (= W221)
binding uridine 5'-triphosphate: L43 (= L43), Y112 (≠ F112), G113 (= G113), H114 (= H114), G115 (≠ D115), F116 (≠ L116), T117 (≠ E117), K247 (= K247), V248 (= V248), A249 (≠ C249), E253 (= E253), I254 (≠ V254), R257 (= R257)

4ho5A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with tdp-glucose
68% identity, 99% coverage: 1:285/288 of query aligns to 1:285/288 of 4ho5A

query
sites
4ho5A

M

M

K

K

G

G

I

I

L
|
L

A
x
S

G
|
G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

L

L

T

T

Q

K

V

V

T

V

S

S

K

K

Q
|
Q

L

L

P

P

V

V

F

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

L

L

S

S

T

V

L

L

M

M

L
|
L

A

A

G

G

I

I

R

K

D

D

I

I

L

L

I

I

T

S

T

T

P

P

D

E

D

D

Q

T

P

P

Q

R

F

F

R

E

R

Q

L

L

G

G

D

G

G

G

S

S

Q

E

W

L

G

G

I

I

A

S

L

L

S

S

Y

Y

A

A

E

V

Q
|
Q

P

S

K

S

P
|
P

E
x
D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

L

I

I

I

G

G

R

E

E

E

F

F

V

I

G

G

R

D

D

D

D

N

V

V

C

A

L

L

I

V

L
|
L

G
|
G

D
|
D

N
|
N

I

I

F

F

F
x
Y

G
|
G

H
|
H

D
x
G

L
x
F

E
x
T

P

E

A

L

L

L

L

Q

R

R

A

A

G

N

R

R

A

K

E

S

G

G

A

A

T

T

V

I

F

F

G

G

Y

Y

H

N

V

V

R

K

D

D

P

P

E

Q

R

R

Y
x
F

G
|
G

V

V

I

V

S

E

F

F

D

D

A

E

E

K

G

G

V

K

P

V

V

I

A

S

I

I

E

E

E
|
E

K
|
K

P

P

T

E

V

E

P

P

K

K

S

S

N

S

Y

Y

A

A

V
|
V

T

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

A

R

V

V

L

V

D

D

I

I

A

A

A

K

T

N

I

I

R

T

P

P

S

S

A

A

R

R

G

G

E
|
E

L

L

E

E

I

I

T

T

D

D

V

V

N

N

N

K

V

A

Y

Y

L

L

E

E

R

L

R

G

R

E

L

L

A

H

V

V

E

E

R

L

L

L

G
|
G

R
|
R

G
|
G

Y

F

A

A

W
|
W

F

L

D

D

T

T

G

G

T

T

H

H

E

E

S

S

L

L

Q

Q

A

A

A

S

E

Q

F

F

V

I

Q

E

T

T

I

I

E

E

A

K

R

R

Q

Q

G

S

L

L

K
|
K

V
|
V

C
x
A

S

C

P

L

E

E

E
|
E

V
x
I

A

A

W

Y

R
|
R

K

M

G

G

F

Y

I

I

D

S

G

R

E

E

Q

Q

L

L

A

I

R

K

I

L

A

A

R

E

P

P

L

L

A

M

K

K

S

N

G

E

Y

Y

G

G

N

Q

Y

Y

L

L

L

M

N

N

L

L

binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: L6 (= L6), S7 (≠ A7), G8 (= G8), G9 (= G9), Q24 (= Q24), L43 (= L43), Q80 (= Q80), P83 (= P83), D84 (≠ E84), G85 (= G85), L86 (= L86), L106 (= L106), G107 (= G107), D108 (= D108), N109 (= N109), Y112 (≠ F112), G113 (= G113), H114 (= H114), G115 (≠ D115), F116 (≠ L116), T117 (≠ E117), F143 (≠ Y143), G144 (= G144), E159 (= E159), K160 (= K160), V170 (= V170), E194 (= E194), G216 (= G216), R217 (= R217), G218 (= G218), W221 (= W221), K247 (= K247), V248 (= V248), A249 (≠ C249), E253 (= E253), I254 (≠ V254), R257 (= R257)

4ho3A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with thymidine triphosphate
68% identity, 99% coverage: 1:285/288 of query aligns to 1:285/288 of 4ho3A

query
sites
4ho3A

M

M

K

K

G

G

I

I

L
|
L

A
x
S

G
|
G

S
|
S

G
|
G

T
|
T

R
|
R

L

L

Y

Y

P

P

L

L

T

T

Q

K

V

V

T

V

S

S

K
|
K

Q
|
Q

L

L

P

P

V

V

F

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

L

L

S

S

T

V

L

L

M

M

L
|
L

A

A

G

G

I

I

R

K

D

D

I

I

L

L

I

I

T

S

T

T

P

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D

D

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G

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G

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L

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Y

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A

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A

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A

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L

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I

G

G

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E

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L

binding thymidine-5'-triphosphate: L6 (= L6), S7 (≠ A7), G8 (= G8), G9 (= G9), S10 (= S10), G11 (= G11), T12 (= T12), R13 (= R13), K23 (= K23), Q24 (= Q24), L43 (= L43), Q80 (= Q80), P83 (= P83), D84 (≠ E84), G85 (= G85), L86 (= L86), L106 (= L106), G107 (= G107), D108 (= D108), Y112 (≠ F112), G113 (= G113), H114 (= H114), G115 (≠ D115), F116 (≠ L116), T117 (≠ E117), E194 (= E194), K247 (= K247), V248 (= V248), A249 (≠ C249), E253 (= E253), I254 (≠ V254), R257 (= R257)

5ifyA Crystal structure of glucose-1-phosphate thymidylyltransferase from burkholderia vietnamiensis in complex with 2 -deoxyuridine-5'- monophosphate and 2'-deoxy-thymidine-b-l-rhamnose
67% identity, 99% coverage: 2:287/288 of query aligns to 3:288/293 of 5ifyA

query
sites
5ifyA

K

K

G

G

I

I

L
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L

A
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A

G
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G

S

S

G

G

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T

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