SitesBLAST

Comparing Ac3H11_1055 FitnessBrowser__acidovorax_3H11:Ac3H11_1055 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Found 20 (the maximum) hits to proteins with known functional sites

5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
32% identity, 98% coverage: 5:259/259 of query aligns to 6:257/259 of 5zaiC

• active site: A65 (≠ G64), F70 (≠ L69), S82 (= S83), R86 (≠ H88), G110 (≠ A112), E113 (≠ S115), P132 (≠ A134), E133 (≠ Y135), I138 (≠ L140), P140 (= P142), G141 (≠ D143), A226 (≠ G228), F236 (≠ L238)
• binding coenzyme a: D23 (≠ E22), K24 (≠ R23), L25 (≠ R24), A27 (= A26), A63 (≠ S62), G64 (= G63), A65 (≠ G64), D66 (≠ N65), I67 (≠ L66), L108 (≠ A110), G109 (= G111), P132 (≠ A134), E133 (≠ Y135), R166 (= R168), F248 (= F250), K251 (= K253)

6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
36% identity, 97% coverage: 10:259/259 of query aligns to 10:255/257 of 6slbAAA

• active site: Q64 (≠ G64), F69 (≠ L69), L80 (≠ I84), N84 (≠ H88), A108 (= A112), S111 (= S115), A130 (= A134), F131 (≠ Y135), L136 (= L140), P138 (= P142), D139 (= D143), A224 (≠ G228), G234 (≠ L238)
• binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: L24 (≠ R24), A26 (= A26), R58 (≠ G58), A62 (≠ S62), G63 (= G63), Q64 (≠ G64), D65 (≠ N65), L66 (= L66), Y76 (≠ V79), H79 (≠ Q82), Y83 (≠ L87), V104 (≠ A108), A106 (= A110), G107 (= G111), A108 (= A112), A130 (= A134), F131 (≠ Y135), I134 (≠ V138), D139 (= D143)

6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
34% identity, 97% coverage: 10:259/259 of query aligns to 7:243/245 of 6slaAAA

• active site: Q61 (≠ P77), L68 (≠ I84), N72 (≠ H88), A96 (= A112), S99 (= S115), A118 (= A134), F119 (≠ Y135), L124 (= L140), P126 (= P142), N127 (≠ D143), A212 (≠ G228), G222 (≠ L238)
• binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: K20 (≠ R23), L21 (≠ R24), A23 (= A26), R55 (≠ G58), A59 (≠ S62), G60 (= G63), Q61 (≠ P77), D62 (= D78), L63 (≠ V79), L68 (≠ I84), Y71 (≠ L87), V92 (≠ A108), A94 (= A110), G95 (= G111), A96 (= A112), A118 (= A134), F119 (≠ Y135), I122 (≠ V138), L124 (= L140), N127 (≠ D143), F234 (= F250), K237 (= K253)

5jbxC Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
33% identity, 100% coverage: 1:259/259 of query aligns to 4:259/261 of 5jbxC

• active site: A67 (≠ G64), R72 (≠ N72), L84 (≠ I84), R88 (≠ H88), G112 (≠ A112), E115 (≠ S115), T134 (≠ A134), E135 (≠ Y135), I140 (≠ L140), P142 (= P142), G143 (≠ D143), A228 (≠ G228), L238 (= L238)
• binding coenzyme a: F250 (= F250), K253 (= K253)

O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
30% identity, 97% coverage: 10:259/259 of query aligns to 15:264/266 of O53561

• K135 (≠ V130) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
• 135:142 (vs. 130:137, 13% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
• K142 (≠ N137) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.

7borA Structure of pseudomonas aeruginosa coa-bound odaa (see paper)
32% identity, 96% coverage: 5:253/259 of query aligns to 4:247/247 of 7borA

• active site: N63 (≠ G64), F68 (≠ L69), D77 (≠ A81), G81 (≠ H88), I105 (≠ A112), T108 (≠ S115), F128 (≠ Y135), L133 (= L140), P135 (= P142), E136 (≠ D143), A222 (≠ G228), L232 (= L238)
• binding coenzyme a: D21 (≠ E22), K22 (≠ R23), K23 (≠ R24), A25 (= A26), S61 (= S62), N63 (≠ G64), D64 (≠ N65), I65 (≠ L66), P101 (≠ A108), V103 (≠ A110), P127 (≠ A134), F128 (≠ Y135), L131 (≠ V138), F244 (= F250), M245 (≠ L251), R247 (≠ K253)

2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
28% identity, 95% coverage: 15:259/259 of query aligns to 19:258/260 of 2hw5C

• active site: A68 (≠ G64), M73 (≠ L69), S83 (= S83), L87 (= L87), G111 (≠ A112), E114 (≠ S115), P133 (≠ A134), E134 (≠ Y135), T139 (≠ L140), P141 (= P142), G142 (≠ D143), K227 (≠ G228), F237 (≠ L238)
• binding crotonyl coenzyme a: K26 (≠ E22), A27 (≠ R23), L28 (≠ R24), A30 (= A26), K62 (≠ G58), A66 (≠ S62), A68 (≠ G64), D69 (≠ N65), I70 (≠ L66), K71 (≠ Q67), Y107 (≠ A108), F109 (≠ A110)

3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
30% identity, 94% coverage: 15:258/259 of query aligns to 15:249/250 of 3q0gD

• active site: A64 (≠ G64), M69 (≠ L69), T75 (≠ S83), F79 (≠ L87), G103 (≠ A112), E106 (≠ S115), P125 (≠ A134), E126 (≠ Y135), V131 (≠ L140), P133 (= P142), G134 (≠ D143), L219 (≠ G228), F229 (≠ L238)
• binding Butyryl Coenzyme A: F225 (= F234), F241 (= F250), K244 (= K253)

1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
29% identity, 95% coverage: 15:259/259 of query aligns to 19:256/258 of 1mj3A

• active site: A68 (≠ G64), M73 (≠ L69), S83 (≠ E85), L85 (= L87), G109 (≠ A112), E112 (≠ S115), P131 (≠ A134), E132 (≠ Y135), T137 (≠ L140), P139 (= P142), G140 (≠ D143), K225 (≠ G228), F235 (≠ L238)
• binding hexanoyl-coenzyme a: K26 (≠ E22), A27 (≠ R23), L28 (≠ R24), A30 (= A26), K62 (≠ G58), A66 (≠ S62), G67 (= G63), A68 (≠ G64), D69 (≠ N65), I70 (≠ L66), K71 (≠ Q67), M73 (≠ L69), W88 (= W90), Y105 (≠ A108), L107 (≠ A110), G108 (= G111), G109 (≠ A112), E112 (≠ S115), P131 (≠ A134), E132 (≠ Y135), L135 (≠ V138), G140 (≠ D143), A141 (≠ G144), F231 (= F234), F247 (= F250), K250 (= K253)

1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
29% identity, 95% coverage: 15:259/259 of query aligns to 17:256/258 of 1ey3A

• active site: A66 (≠ G64), M71 (≠ L69), S81 (= S83), L85 (= L87), G109 (≠ A112), E112 (≠ S115), P131 (≠ A134), E132 (≠ Y135), T137 (≠ L140), P139 (= P142), G140 (≠ D143), K225 (≠ G228), F235 (≠ L238)
• binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ E22), A25 (≠ R23), L26 (≠ R24), A28 (= A26), A64 (≠ S62), G65 (= G63), A66 (≠ G64), D67 (≠ N65), I68 (≠ L66), K69 (≠ Q67), L85 (= L87), W88 (= W90), Y105 (≠ A108), L107 (≠ A110), G108 (= G111), G109 (≠ A112), P131 (≠ A134), E132 (≠ Y135), L135 (≠ V138), G140 (≠ D143), A141 (≠ G144), R165 (= R168), F231 (= F234), F247 (= F250), K250 (= K253)

1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
29% identity, 95% coverage: 15:259/259 of query aligns to 19:258/260 of 1dubA

• active site: A68 (≠ G64), M73 (≠ L69), S83 (= S83), L87 (= L87), G111 (≠ A112), E114 (≠ S115), P133 (≠ A134), E134 (≠ Y135), T139 (≠ L140), P141 (= P142), G142 (≠ D143), K227 (≠ G228), F237 (≠ L238)
• binding acetoacetyl-coenzyme a: K26 (≠ E22), A27 (≠ R23), L28 (≠ R24), A30 (= A26), K62 (≠ G58), A66 (≠ S62), G67 (= G63), A68 (≠ G64), D69 (≠ N65), I70 (≠ L66), K71 (≠ Q67), M73 (≠ L69), Y107 (≠ A108), L109 (≠ A110), G110 (= G111), G111 (≠ A112), E114 (≠ S115), P133 (≠ A134), E134 (≠ Y135), L137 (≠ V138), G142 (≠ D143), F233 (= F234), F249 (= F250), K252 (= K253)

P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
29% identity, 95% coverage: 15:259/259 of query aligns to 49:288/290 of P14604

• E144 (≠ S115) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
• E164 (≠ Y135) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.

Sites not aligning to the query:

• 1:29 modified: transit peptide, Mitochondrion

3q0jA Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
30% identity, 94% coverage: 15:258/259 of query aligns to 16:254/255 of 3q0jA

• active site: A65 (≠ G64), M70 (≠ L69), T80 (≠ S83), F84 (≠ L87), G108 (≠ A112), E111 (≠ S115), P130 (≠ A134), E131 (≠ Y135), V136 (≠ L140), P138 (= P142), G139 (≠ D143), L224 (≠ G228), F234 (≠ L238)
• binding acetoacetyl-coenzyme a: F246 (= F250), K249 (= K253)

3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
30% identity, 94% coverage: 15:258/259 of query aligns to 16:254/255 of 3q0gC

• active site: A65 (≠ G64), M70 (≠ L69), T80 (≠ S83), F84 (≠ L87), G108 (≠ A112), E111 (≠ S115), P130 (≠ A134), E131 (≠ Y135), V136 (≠ L140), P138 (= P142), G139 (≠ D143), L224 (≠ G228), F234 (≠ L238)
• binding coenzyme a: A24 (≠ R23), L25 (≠ R24), A27 (= A26), A63 (≠ S62), A65 (≠ G64), D66 (≠ N65), I67 (≠ L66), K68 (≠ Q67), Y104 (≠ A108), P130 (≠ A134), E131 (≠ Y135), L134 (≠ V138)

3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
30% identity, 94% coverage: 15:258/259 of query aligns to 15:253/256 of 3h81A

• active site: A64 (≠ G64), M69 (≠ L69), T79 (≠ S83), F83 (≠ L87), G107 (≠ A112), E110 (≠ S115), P129 (≠ A134), E130 (≠ Y135), V135 (≠ L140), P137 (= P142), G138 (≠ D143), L223 (≠ G228), F233 (≠ L238)
• binding calcium ion: R171 (≠ A176), S172 (≠ L177), F233 (≠ L238), Q238 (≠ G243)

Q4WF54 Mevalonyl-coenzyme A hydratase sidH; Siderophore biosynthesis protein H; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
30% identity, 100% coverage: 1:258/259 of query aligns to 1:265/270 of Q4WF54