SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ac3H11_1394 FitnessBrowser__acidovorax_3H11:Ac3H11_1394 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
36% identity, 65% coverage: 50:154/162 of query aligns to 74:174/183 of 3nz2C

query
sites
3nz2C

V

I

G

G

E

D

H

H

V

T

V

F

I

I

N

N

A

M

G

N

C

V

V

V

L

M

Y

L

T

D

G

G

N

A

G

P

I

I

H

T

I

I

G

G

N

D

Y

H

V

V

A

L

I

I

A

G

A

P

N

S

C

T

T

Q

F

F

A
x
Y

P

T

V

A

N

S

H
|
H

A

S

Y

L

A

D

D

Y

R

R

S

R

R

R

L

Q

I

A

R

W

E

E

Q

T

G

I

F

C

L

K

P

P

S

-

K

-

G

-

I

I

V

V

I

I

E

E

D

D

V

V

W

W

I

I

G
|
G

A

G

N

N

C

V

V

V

L

I

L

N

D

Q

G

G

A

V

V

T

L

I

R

G

R

A

G

R

C

S

V

V

G
x
A

A
|
A

G
x
N

S

S

I

V

V

V

R
x
N

G

Q

E

D

L

V

L

P

A

P

Y

D

T

T

V

L

Y

V

A
x
G

G

G

Q
x
T

P
|
P

binding acetyl coenzyme *a: Y107 (≠ A83), H111 (= H87), G137 (= G117), A155 (≠ G135), A156 (= A136), N161 (≠ R141), G171 (≠ A151), T173 (≠ Q153), P174 (= P154)
binding magnesium ion: N157 (≠ G137), T173 (≠ Q153)

Sites not aligning to the query:

binding acetyl coenzyme *a: 178, 179

3nz2J Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
36% identity, 65% coverage: 50:154/162 of query aligns to 77:177/185 of 3nz2J

query
sites
3nz2J

V

I

G

G

E

D

H

H

V

T

V

F

I

I

N

N

A

M

G

N

C

V

V

V

L

M

Y

L

T

D

G

G

N

A

G

P

I

I

H

T

I

I

G

G

N

D

Y

H

V

V

A

L

I

I

A
x
G

A

P

N

S

C

T

T

Q

F

F

A
x
Y

P

T

V

A

N

S

H
|
H

A

S

Y

L

A

D

D

Y

R

R

S

R

R

R

L

Q

I

A

R

W

E

E

Q

T

G

I

F

C

L

K

P

P

S

-

K

-

G

-

I

I

V

V

I

I

E

E

D

D

V

V

W
|
W

I

I

G
|
G

A

G

N

N

C

V

V

V

L

I

L

N

D

Q

G

G

A

V

V

T

L

I

R

G

R

A

G

R

C

S

V

V

G
x
A

A
|
A

G

N

S

S

I

V

V

V

R
x
N

G

Q

E

D

L

V

L

P

A

P

Y

D

T

T

V

L

Y

V

A
x
G

G

G

Q
x
T

P
|
P

binding acetyl coenzyme *a: G104 (≠ A77), Y110 (≠ A83), H114 (= H87), W138 (= W115), G140 (= G117), A158 (≠ G135), A159 (= A136), N164 (≠ R141), G174 (≠ A151), T176 (≠ Q153), P177 (= P154)

Sites not aligning to the query:

binding acetyl coenzyme *a: 182

3ectA Crystal structure of the hexapeptide-repeat containing- acetyltransferase vca0836 from vibrio cholerae
36% identity, 65% coverage: 50:154/162 of query aligns to 67:167/176 of 3ectA

query
sites
3ectA

V

I

G

G

E

D

H

H

V

T

V

F

I

I

N

N

A

M

G

N

C

V

V

V

L

M

Y

L

T

D

G

G

N

A

G

P

I

I

H

T

I

I

G

G

N

D

Y

H

V

V

A

L

I

I

A

G

A

P

N

S

C

T

T

Q

F

F

A

Y

P

T

V

A

N

S

H

H

A

S

Y

L

A

D

D

Y

R

R

S

R

R

R

L

Q

I

A

R

W

E

E

Q

T

G

I

F

C

L

K

P

P

S

-

K

-

G

-

I

I

V

V

I

I

E

E

D

D

V

V

W

W

I

I

G

G

A

G

N

N

C

V

V

V

L

I

L

N

D

Q

G
|
G

A

V

V

T

L

I

R

G

R

A

G

R

C

S

V

V

G

A

A

A

G

N

S

S

I

V

V

V

R

N

G

Q

E
x
D

L

V

L

P

A

P

Y

D

T

T

V

L

Y

V

A

G

G

G

Q

T

P

P

binding calcium ion: G138 (= G125), D156 (≠ E143)

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
30% identity, 69% coverage: 47:158/162 of query aligns to 74:181/190 of 5u2kA

query
sites
5u2kA

D

N

L

V

V

K

V

L

G

G

E

K

H

N

V

V

V

Y

I

V

N

N

A

T

G

N

C

C

V

Y

L

F

Y
x
M

T

D

G

G

N

G

G

Q

I

I

H

T

I

I

G

G

N

D

Y

N

V

V

A

F

I

I

A

G

A

P

N

N

C

C

T

G

F

F

A
x
Y

P

T

V
x
A

N

T

H
|
H

A

P

Y

L

A

N

D

F

R

H

S

H

R

R

L

-

I

-

R

-

E

N

Q

E

G

G

F

F

L

E

P

K

S

A

K

-

G

G

G

P

I

I

V

H

I

I

E

G

D

S

D

N

V

T

W

W

I

F

G

G

A

G

N

H

C

V

V

A

L

V

L
|
L

D

P

G

G

A

V

V

T

L

I

R

G

R

E

G

G

C

S

V

V

V

I

G

G

A

A

G

G

S

S

I

V

V

V

R
x
T

G
x
K

E

D

L

I

L

P

A

P

Y

H

T

S

V

L

Y

A

A

V

G

G

Q

N

P

P

L

C

A

K

A

V

V

V

binding coenzyme a: M90 (≠ Y63), Y110 (≠ A83), A112 (≠ V85), H114 (= H87), L146 (= L123), T164 (≠ R141), K165 (≠ G142)

6x3cA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
24% identity, 96% coverage: 6:161/162 of query aligns to 10:166/207 of 6x3cA

query
sites
6x3cA

P

P

T

I

A

K

K

G

V

N

S

R

H

N

L
|
L

A

Q

D

F

I

I

E

K

D

P

S

T

V

I

R

T

G

N

T

E

R

N

I

I

V

L

V

V

G

G

A

E

H

Y

S

S

V
x
Y

I

Y

D

D

S

S

F

-

V

-

K

-

V

-

K

K

P

R

A

G

G

E

G

S

S

F

G

E

D

D

L

Q

V

V

V

L

G
x
Y

E

H

H
x
Y

V

E

V

V

I

I

N

-

A

-

G

-

C

-

V

-

L

-

Y

-

T

-

G

G

N

D

G

K

I

L

H

I

I

I

G

G

N

R

Y

F

V

C

A

S

I

I

A
x
G

A

P

N

G

C

T

T

T

F

F

-

I

-

M

-

N

-
x
G

-

A

-
x
N

-
x
H

-

R

-

M

-

D

-

G

-

S

-

T

A

Y

P

P

V

F

N
x
H

H
x
L

A

F

Y

R

A

M

D

G

R

W

S

E

R

K

L

Y

I
x
M

R
x
P

E

S

Q
x
L

G

K

F

D

L

L

P

P

S

L

K

K

G

G

G

D

I

I

V

E

I

I

E

G

D

N

D

D

V

V

W
|
W

I

I

G
|
G

A
x
R

N

D

C

V

V

T

L

I

L
x
M

D

P

G

G

A

V

V

K

L

I

R

G

R

D

G

G

C

A

V

I

G
x
A

A
|
A

G

E

S

A

I

V

V

V

R

T

G

K

E

N

L

V

L

A

A

P

Y

Y

T

S

V

I

Y

V

A

G

G

G

Q

N

P

P

L

L

A

K

A

F

V

I

G

R

E

K

R

R

binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: L17 (= L13), Y36 (≠ V32), Y53 (≠ H53), G78 (vs. gap), N80 (vs. gap), H81 (vs. gap), H91 (≠ N86), L92 (≠ H87), M101 (≠ I96), P102 (≠ R97), L104 (≠ Q99)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: Y51 (≠ G51), G69 (≠ A77), N80 (vs. gap), H81 (vs. gap), W120 (= W115), G122 (= G117), R123 (≠ A118), M128 (≠ L123), A140 (≠ G135), A141 (= A136)

4husA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with virginiamycin m1 (see paper)
24% identity, 96% coverage: 6:161/162 of query aligns to 10:166/212 of 4husA

query
sites
4husA

P

P

T

I

A

K

K

G

V

N

S

R

H

N

L
|
L

A

Q

D

F

I

I

E

K

D

P

S

T

V

I

R

T

G

N

T

E

R

N

I

I

V

L

V

V

G

G

A

E

H

Y

S

S

V
x
Y

I

Y

D
|
D

S

S

F

-

V

-

K

-

V

-

K

K

P

R

A

G

G

E

G

S

S

F

G

E

D

D

L

Q

V

V

V

L

G

Y

E

H

H

Y

V

E

V

V

I

I

N

-

A

-

G

-

C

-

V

-

L

-

Y

-

T

-

G

G

N

D

G

K

I

L

H

I

I

I

G

G

N

R

Y

F

V

C

A

S

I

I

A

G

A

P

N

G

C

T

T

T

F

F

-

I

-

M

-

N

-

G

-

A

-

N

-

H

-

R

-

M

-

D

-

G

-

S

-

T

A

Y

P

P

V

F

N

H

H

L

A

F

Y

R

A

M

D

G

R

W

S

E

R

K

L

Y

I

M

R

P

E

S

Q

L

G

K

F

D

L

L

P

P

S

L

K

K

G

G

G

D

I

I

V

E

I

I

E

G

D

N

D

D

V

V

W

W

I

I

G

G

A

R

N

D

C

V

V

T

L

I

L

M

D

P

G

G

A

V

V

K

L

I

R

G

R

D

G

G

C

A

V

I

G

A

A

A

G

E

S

A

I

V

V

V

R

T

G

K

E

N

L

V

L

A

A

P

Y

Y

T

S

V

I

Y

V

A

G

G

G

Q

N

P

P

L

L

A

K

A

F

V

I

G

R

E

K

R

R

binding virginiamycin m1: L17 (= L13), Y36 (≠ V32), D38 (= D34)

4hurA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with acetyl coenzyme a (see paper)
24% identity, 96% coverage: 6:161/162 of query aligns to 10:166/211 of 4hurA

query
sites
4hurA

P

P

T

I

A

K

K

G

V

N

S

R

H

N

L

L

A

Q

D

F

I

I

E

K

D

P

S

T

V

I

R

T

G

N

T

E

R

N

I

I

V

L

V

V

G

G

A

E

H

Y

S

S

V

Y

I

Y

D

D

S

S

F

-

V

-

K

-

V

-

K

K

P

R

A

G

G

E

G

S

S

F

G

E

D

D

L

Q

V

V

V

L

G
x
Y

E

H

H

Y

V

E

V

V

I

I

N

-

A

-

G

-

C

-

V

-

L

-

Y

-

T

-

G

G

N

D

G

K

I

L

H

I

I

I

G

G

N

R

Y

F

V

C

A
x
S

I
|
I

A
x
G

A

P

N

G

C

T

T

T

F

F

-

I

-

M

-

N

-

G

-
x
A

-
x
N

-

H

-

R

-

M

-

D

-

G

-

S

-

T

A

Y

P

P

V

F

N

H

H

L

A

F

Y

R

A

M

D

G

R

W

S

E

R

K

L

Y

I

M

R

P

E

S

Q

L

G

K

F

D

L

L

P

P

S

L

K
|
K

G

G

G

D

I

I

V

E

I

I

E

G

D

N

D

D

V

V

W
|
W

I
|
I

G
|
G

A
x
R

N

D

C

V

V

T

L

I

L
x
M

D

P

G

G

A

V

V

K

L

I

R

G

R

D

G

G

C

A

V

I

G
x
A

A
|
A

G

E

S

A

I

V

V

V

R
x
T

G

K

E

N

L

V

L

A

A

P

Y

Y

T

S

V

I

Y

V

A
x
G

G

G

Q

N

P
|
P

L

L

A

K

A

F

V
x
I

G
x
R

E

K

R

R

binding acetyl coenzyme *a: Y51 (≠ G51), S67 (≠ A75), I68 (= I76), G69 (≠ A77), A79 (vs. gap), N80 (vs. gap), K110 (= K105), W120 (= W115), I121 (= I116), G122 (= G117), R123 (≠ A118), M128 (≠ L123), A140 (≠ G135), A141 (= A136), T146 (≠ R141), G156 (≠ A151), P159 (= P154), I163 (≠ V158), R164 (≠ G159)

6x3jA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f0224 (46) (see paper)
24% identity, 96% coverage: 6:161/162 of query aligns to 10:166/206 of 6x3jA

query
sites
6x3jA

P

P

T

I

A

K

K

G

V

N

S

R

H

N

L

L

A

Q

D

F

I

I

E

K

D

P

S

T

V

I

R

T

G

N

T

E

R

N

I

I

V

L

V

V

G

G

A

E

H

Y

S

S

V

Y

I

Y

D

D

S

S

F

-

V

-

K

-

V

-

K

K

P

R

A

G

G

E

G

S

S

F

G

E

D

D

L

Q

V

V

V

L

G
x
Y

E

H

H
x
Y

V

E

V
|
V

I

I

N

-

A

-

G

-

C

-

V

-

L

-

Y

-

T

-

G

G

N

D

G

K

I

L

H

I

I

I

G

G

N

R

Y

F

V

C

A

S

I

I

A
x
G

A

P

N

G

C

T

T

T

F

F

-

I

-

M

-

N

-

G

-
x
A

-

N

-
x
H

-

R

-

M

-

D

-

G

-

S

-

T

A

Y

P

P

V

F

N
x
H

H
x
L

A

F

Y

R

A

M

D

G

R

W

S

E

R

K

L

Y

I
x
M

R
x
P

E

S

Q

L

G

K

F

D

L
|
L

P

P

S

L

K

K

G

G

G

D

I

I

V

E

I

I

E

G

D

N

D

D

V

V

W
|
W

I

I

G
|
G

A

R

N

D

C

V

V

T

L

I

L

M

D

P

G

G

A

V

V

K

L

I

R

G

R

D

G

G

C

A

V

I

G

A

A

A

G

E

S

A

I

V

V

V

R

T

G

K

E

N

L

V

L

A

A

P

Y

Y

T

S

V

I

Y

V

A

G

G

G

Q

N

P

P

L

L

A

K

A

F

V

I

G

R

E

K

R

R

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: Y51 (≠ G51), Y53 (≠ H53), V55 (= V55), H91 (≠ N86), L92 (≠ H87), M101 (≠ I96), P102 (≠ R97), L107 (= L102)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (≠ A77), A79 (vs. gap), H81 (vs. gap), W120 (= W115), G122 (= G117)

6x3cE Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
24% identity, 96% coverage: 6:161/162 of query aligns to 10:166/203 of 6x3cE

query
sites
6x3cE

P

P

T

I

A

K

K

G

V

N

S

R

H

N

L

L

A

Q

D

F

I

I

E

K

D

P

S

T

V

I

R

T

G

N

T

E

R

N

I

I

V

L

V

V

G

G

A

E

H

Y

S

S

V

Y

I

Y

D

D

S

S

F

-

V

-

K

-

V

-

K

K

P

R

A

G

G

E

G

S

S

F

G

E

D

D

L

Q

V

V

V

L

G
x
Y

E

H

H
x
Y

V

E

V

V

I

I

N

-

A

-

G

-

C

-

V

-

L

-

Y

-

T

-

G

G

N

D

G

K

I

L

H

I

I

I

G

G

N

R

Y

F

V

C

A

S

I

I

A
x
G

A

P

N

G

C

T

T

T

F

F

-

I

-

M

-

N

-
x
G

-

A

-
x
N

-
x
H

-

R

-
x
M

-

D

-

G

-

S

-

T

A

Y

P

P

V

F

N
x
H

H
x
L

A

F

Y

R

A

M

D

G

R

W

S

E

R

K

L

Y

I
x
M

R
x
P

E

S

Q

L

G

K

F

D

L

L

P

P

S

L

K

K

G

G

G

D

I

I

V

E

I

I

E

G

D

N

D

D

V

V

W
|
W

I

I

G
|
G

A

R

N

D

C

V

V

T

L

I

L

M

D

P

G

G

A

V

V

K

L

I

R

G

R

D

G

G

C

A

V

I

G
x
A

A
|
A

G

E

S

A

I

V

V

V

R

T

G

K

E

N

L

V

L

A

A

P

Y

Y

T

S

V

I

Y

V

A

G

G

G

Q
x
N

P
|
P

L

L

A

K

A

F

V

I

G

R

E

K

R

R

binding magnesium ion: N158 (≠ Q153), P159 (= P154)
binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: Y53 (≠ H53), G78 (vs. gap), N80 (vs. gap), H81 (vs. gap), H91 (≠ N86), L92 (≠ H87), M101 (≠ I96), P102 (≠ R97)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: Y51 (≠ G51), G69 (≠ A77), H81 (vs. gap), M83 (vs. gap), W120 (= W115), G122 (= G117), A140 (≠ G135), A141 (= A136)

1kk4A Crystal structure of vat(d) in complex with acetyl-coa (see paper)
35% identity, 37% coverage: 102:161/162 of query aligns to 108:167/205 of 1kk4A

query
sites
1kk4A

L

L

P

P

S

I

K
|
K

G

G

G

D

I

T

V

I

I

I

E

G

D

N

D

D

V

V

W
|
W

I

I

G
|
G

A
x
K

N

D

C

V

V

V

L

I

L

M

D

P

G

G

A

V

V

K

L

I

R

G

R

D

G

G

C

A

V

I

V

V

G
x
A

A
|
A

G

N

S

S

I

V

V

V

R
x
V

G
x
K

E

D

L

I

L

A

A

P

Y

Y

T

M

V
x
L

Y

A

A
x
G

G
|
G

Q

N

P
|
P

L

A

A

N

A

E

V
x
I

G

K

E

Q

R

R

binding acetyl coenzyme *a: K111 (= K105), W121 (= W115), G123 (= G117), K124 (≠ A118), A141 (≠ G135), A142 (= A136), V147 (≠ R141), K148 (≠ G142), L155 (≠ V149), G157 (≠ A151), G158 (= G152), P160 (= P154), I164 (≠ V158)

Sites not aligning to the query:

binding acetyl coenzyme *a: 69, 70

1khrA Crystal structure of vat(d) in complex with virginiamycin and coenzyme a (see paper)
35% identity, 37% coverage: 102:161/162 of query aligns to 108:167/206 of 1khrA

query
sites
1khrA

L

L

P

P

S

I

K

K

G

G

G

D

I

T

V

I

I

I

E

G

D

N

D

D

V

V

W
|
W

I

I

G

G

A

K

N

D

C

V

V

V

L

I

L

M

D

P

G

G

A

V

V

K

L

I

R

G

R

D

G

G

C

A

V

I

V

V

G
x
A

A
|
A

G

N

S

S

I

V

V

V

R
x
V

G

K

E

D

L

I

L

A

A

P

Y

Y

T

M

V
x
L

Y

A

A
x
G

G

G

Q

N

P
|
P

L

A

A

N

A

E

V
x
I

G

K

E

Q

R

R

binding coenzyme a: W121 (= W115), A141 (≠ G135), A142 (= A136), V147 (≠ R141), L155 (≠ V149), G157 (≠ A151), P160 (= P154), I164 (≠ V158)

Sites not aligning to the query:

binding coenzyme a: 82, 84
binding virginiamycin m1: 37, 39, 54, 80, 81, 82, 93

3dhoA Structure of streptogramin acetyltransferase in complex with an inhibitor
35% identity, 37% coverage: 102:161/162 of query aligns to 108:167/203 of 3dhoA

query
sites
3dhoA

L

L

P

P

S

I

K

K

G

G

G

D

I

T

V

I

I

I

E

G

D

N

D

D

V

V

W

W

I

I

G

G

A

K

N

D

C

V

V

V

L

I

L

M

D

P

G

G

A

V

V

K

L

I

R

G

R

D

G

G

C

A

V
x
I

V

V

G
x
A

A

A

G

N

S

S

I

V

V

V

R
x
V

G

K

E

D

L

I

L

A

A

P

Y

Y

T

M

V
x
L

Y

A

A
x
G

G

G

Q

N

P
|
P

L

A

A

N

A

E

V
x
I

G

K

E

Q

R

R

binding 6-bromo-N'-[(1Z)-(3,5-dichloro-2-hydroxyphenyl)methylidene]-2-methylquinoline-4-carbohydrazide: I139 (≠ V133), A141 (≠ G135), V147 (≠ R141), L155 (≠ V149), G157 (≠ A151), P160 (= P154), I164 (≠ V158)

Sites not aligning to the query:

binding 6-bromo-N'-[(1Z)-(3,5-dichloro-2-hydroxyphenyl)methylidene]-2-methylquinoline-4-carbohydrazide: 84

P50870 Streptogramin A acetyltransferase; Virginiamycin acetyltransferase D; Vat(D); EC 2.3.1.- from Enterococcus faecium (Streptococcus faecium) (see paper)
35% identity, 37% coverage: 102:161/162 of query aligns to 108:167/209 of P50870

query
sites
P50870

L

L

P

P

S

I

K

K

G

G

G

D

I

T

V

I

I

I

E

G

D

N

D

D

V

V

W

W

I

I

G

G

A

K

N

D

C

V

V

V

L

I

L

M

D

P

G

G

A

V

V

K

L

I

R

G

R

D

G

G

C

A

V

I

V

V

G

A

A

A

G

N

S

S

I

V

V

V

R

V

G

K

E

D

L

I

L

A

A

P

Y

Y

T

M

V

L

Y

A

A

G

G

G

Q

N

P

P

L

A

A

N

A

E

V

I

G

K

E

Q

R

R

Sites not aligning to the query:

82 H→A: 105-fold decrease in activity.

1mrlA Crystal structure of streptogramin a acetyltransferase with dalfopristin (see paper)
35% identity, 37% coverage: 102:161/162 of query aligns to 108:167/204 of 1mrlA

query
sites
1mrlA

L
|
L

P

P

S

I

K

K

G

G

G

D

I

T

V

I

I

I

E

G

D

N

D

D

V

V

W

W

I

I

G

G

A

K

N

D

C

V

V

V

L

I

L

M

D

P

G

G

A

V

V

K

L

I

R

G

R

D

G

G

C

A

V

I

V

V

G

A

A

A

G

N

S

S

I

V

V

V

R

V

G

K

E

D

L

I

L

A

A

P

Y

Y

T

M

V

L

Y

A

A

G

G

G

Q

N

P

P

L

A

A

N

A

E

V

I

G

K

E

Q

R

R

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: L108 (= L102)

Sites not aligning to the query:

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: 37, 39, 54, 56, 81, 82, 93, 102

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
29% identity, 71% coverage: 46:160/162 of query aligns to 73:183/200 of 1krrA

query
sites
1krrA

G

S

D

N

L

I

V

H

V

I

G

G

E

R

H

N

V

F

V

Y

I

A

N
|
N

A

F

G

N

C

L

V

T

L

I

Y

V

T

D

G

D

N

Y

G

T

I

V

H

T

I

I

G

G

N

D

Y

N

V

V

A

L

I
|
I

A
|
A

A
x
P

N

N

C

V

T

T

F

L

A

S

P

V

V
x
T

N

G

H
|
H

A

P

Y

V

A

H

D

H

R

E

S

L

R

R

L

K

I

N

R

G

E

E

Q

M

G

Y

F

S

L

F

P

P

S

-

K

-

G

-

I

I

V

T

I

I

E

G

D

N

V

V

W

W

I

I

G
|
G

A
x
S

N

H

C

V

V

V

L

I

L
x
N

D

P

G

G

A

V

V

T

L

I

R

G

R

D

G

N

C

S

V

V

V

I

G
|
G

A
|
A

G

G

S

S

I

I

V

V

R

T

G

K

E

D

L

I

L

P

A

P

Y

N

T

V

V

V

Y

A

A
|
A

G

G

Q

V

P
|
P

L

C

A

R

A

V

V

I

G
x
R

E

E

binding acetyl coenzyme *a: N84 (= N57), I103 (= I76), A104 (= A77), P105 (≠ A78), T112 (≠ V85), H114 (= H87), G140 (= G117), S141 (≠ A118), N146 (≠ L123), G158 (= G135), A159 (= A136), A174 (= A151), P177 (= P154), R182 (≠ G159)

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
29% identity, 71% coverage: 46:160/162 of query aligns to 74:184/203 of P07464

query
sites
P07464

G

S

D

N

L

I

V

H

V

I

G

G

E

R

H

N

V

F

V

Y

I

A

N
|
N

A

F

G

N

C

L

V

T

L

I

Y

V

T

D

G
x
D

N

Y

G

T

I

V

H

T

I

I

G

G

N

D

Y

N

V

V

A

L

I

I

A

A

A

P

N

N

C

V

T

T

F

L

A

S

P

V

V

T

N

G

H
|
H

A

P

Y

V

A

H

D

H

R

E

S

L

R

R

L

K

I

N

R

G

E

E

Q

M

G

Y

F

S

L

F

P

P

S

-

K

-

G

-

I

I

V

T

I

I

E

G

D

N

V

V

W

W

I

I

G

G

A
x
S

N

H

C

V

V

V

L

I

L

N

D

P

G

G

A

V

V

T

L

I

R

G

R

D

G

N

C

S

V

V

V

I

G

G

A
|
A

G

G

S

S

I

I

V

V

R
x
T

G
x
K

E

D

L

I

L

P

A

P

Y

N

T

V

V

V

Y

A

A

A

G

G

Q

V

P

P

L

C

A
x
R

A

V

V

I

G
x
R

E

E

N85 (= N57) binding in other chain; binding in other chain
D93 (≠ G65) binding
H115 (= H87) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A118) binding in other chain
A160 (= A136) binding in other chain
TK 165:166 (≠ RG 141:142) binding
R180 (≠ A156) binding
R183 (≠ G159) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding
71 binding

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
29% identity, 71% coverage: 46:160/162 of query aligns to 73:183/201 of 1krvA

query
sites
1krvA

G

S

D

N

L

I

V

H

V

I

G

G

E

R

H

N

V

F

V
x
Y

I

A

N
|
N

A

F

G

N

C

L

V

T

L

I

Y
x
V

T

D

G
x
D

N

Y

G

T

I

V

H

T

I

I

G

G

N

D

Y

N

V

V

A

L

I

I

A
|
A

A

P

N

N

C

V

T

T

F

L

A
x
S

P

V

V
x
T

N

G

H

H

A

P

Y

V

A

H

D

H

R

E

S

L

R

R

L

K

I

N

R

G

E

E

Q
x
M

G

Y

F

S

L

F

P

P

S

-

K

-

G

-

I

I

V

T

I

I

E

G

D

N

V

V

W
|
W

I

I

G
|
G

A
x
S

N

H

C

V

V

V

L

I

L
x
N

D

P

G

G

A

V

V

T

L

I

R

G

R

D

G

N

C

S

V

V

V

I

G

G

A
|
A

G

G

S

S

I

I

V

V

R

T

G

K

E

D

L

I

L

P

A

P

Y

N

T

V

V

V

Y

A

A

A

G

G

Q

V

P
|
P

L

C

A
x
R

A

V

V

I

G
x
R

E

E

binding 4-nitrophenyl beta-D-galactopyranoside: Y82 (≠ V55), N84 (= N57), V90 (≠ Y63), D92 (≠ G65), M126 (≠ Q99)
binding coenzyme a: A104 (= A77), S110 (≠ A83), T112 (≠ V85), W138 (= W115), G140 (= G117), S141 (≠ A118), N146 (≠ L123), A159 (= A136), P177 (= P154), R179 (≠ A156), R182 (≠ G159)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25, 70

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
29% identity, 71% coverage: 46:160/162 of query aligns to 73:183/201 of 1kruA

query
sites
1kruA

G

S

D

N

L

I

V

H

V

I

G

G

E

R

H

N

V

F

V
x
Y

I

A

N
|
N

A

F

G

N

C

L

V

T

L

I

Y
x
V

T

D

G
x
D

N

Y

G

T

I

V

H

T

I

I

G

G

N

D

Y

N

V

V

A
x
L

I

I

A

A

A

P

N

N

C

V

T

T

F

L

A

S

P

V

V

T

N

G

H
|
H

A

P

Y

V

A

H

D

H

R

E

S

L

R

R

L

K

I

N

R

G

E

E

Q
x
M

G

Y

F

S

L

F

P

P

S

-

K

-

G

-

I

I

V

T

I

I

E

G

D

N

V

V

W
|
W

I

I

G
|
G

A
x
S

N

H

C

V

V

V

L

I

L

N

D

P

G

G

A

V

V

T

L

I

R

G

R

D

G

N

C

S

V

V

V

I

G
|
G

A
|
A

G

G

S

S

I

I

V

V

R

T

G

K

E

D

L

I

L

P

A

P

Y

N

T

V

V

V

Y

A

A

A

G

G

Q

V

P
|
P

L

C

A

R

A

V

V

I

G
x
R

E

E

binding coenzyme a: H114 (= H87), W138 (= W115), G140 (= G117), S141 (≠ A118), G158 (= G135), A159 (= A136), P177 (= P154), R182 (≠ G159)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: Y82 (≠ V55), Y82 (≠ V55), N84 (= N57), V90 (≠ Y63), D92 (≠ G65), L102 (≠ A75), H114 (= H87), M126 (≠ Q99)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25, 62, 70

4isxA The crystal structure of maltose o-acetyltransferase from clostridium difficile 630 in complex with acetyl-coa
30% identity, 65% coverage: 50:154/162 of query aligns to 77:177/186 of 4isxA

query
sites
4isxA

V

V

G

G

E

E

H

N

V

F

I

A

N
|
N

A

Y

G

D

C

C

V

I

L

F

Y

L

T

D

G

V

N

C

G

K

I

I

H

E

I

I

G

G

N

D

Y

N

V

V

A

M

I

L

A
|
A

A

P

N

N

C

-

T

-

F

-

A

V

P

Q

V

I

N

Y

H

T

A

A

Y

Y

A

H

D

P

R

I

S

D

R

A

L

Q

I

L

R

R

E

N

Q

S

G

G

F

-

L

I

P

E

S

Y

K

G

G

S

G

P

I

V

V

K

I

I

E

G

D

D

D

N

V

V

W
|
W

I

I

G
|
G

A
x
G

N

G

C

V

V

I

L

I

L

T

D

P

G

G

A

I

V

T

L

I

R

G

R

D

G

N

C

V

V

V

V

I

G

G

A
|
A

G

G

S

S

I

V

V

V

R

T

G

K

E

D

L

I

L

P

A

P

Y

N

T

T

V

V

Y

A

A
x
V

G

G

Q

N

P

P

binding acetyl coenzyme *a: N84 (= N57), A104 (= A77), W138 (= W115), G140 (= G117), G141 (≠ A118), A159 (= A136), V174 (≠ A151)

Sites not aligning to the query:

binding acetyl coenzyme *a: 182

6pubA Crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with crystal violet
25% identity, 90% coverage: 16:161/162 of query aligns to 16:165/210 of 6pubA

query
sites
6pubA

I

L

E

D

D

Q

S

Q

V

V

R

T

G

N

T

P

R

N

I

I

V

I

V

V

G

G

A

K

H

H

S

S

V

Y

I

Y

D

S

S

G

F
x
Y

V
x
Y

K

H

-

G

-

H

-

S

-

F

-

D

-

C

-

V

-

R

-

Y

V

L

K

H

P

P

A

E

G

R

G

D

S

D

G

V

D

D

-

K

L

L

V

V

V

I

G

G

E

S

H

F

V

C

V

S

I

I

N

G

A

S

G

G

C

A

V

V

-

F

L

M

Y

M

T

A

G

G

N

N

G

Q

I

G

H

H

I

R

G

S

N

D

Y

W

V

I

A

S

I

T

A

F

A

P

N

-

C

-

T

F

F

F

A

Y

P

Q

V

D

N

N

H

D

A

N

Y

F

A

A

D

D

R

A

S

-

R

-

L

-

I

-

R

-

E

R

Q

D

G

G

F

F

L

T

P

R

S

S

K

-

G

G

G

D

I

T

V

I

I

I

E

G

D

H

D

D

V

V

W

W

I

I

G

G

A

T

N

E

C

A

V

M

L

I

L

M

D

P

G

G

A

V

V

K

L

I

R

G

R

H

G

G

C

A

V

I

G

A

A

S

G

R

S

S

I

V

V

V

R

T

G

K

E

D

L

V

L

A

A

P

Y

Y

T

E

V

V

Y

V

A

G

G

S

Q

N

P

P

L

A

A

K

A

H

V

I

G

K

E

F

R

R

binding crystal violet: Y36 (≠ F36), Y37 (≠ V37)

Sites not aligning to the query:

binding crystal violet: 4, 7, 9, 10, 11

Query Sequence

>Ac3H11_1394 FitnessBrowser__acidovorax_3H11:Ac3H11_1394
VIELHPTAKVSHLADIEDSVRGTRIVVGAHSVIDSFVKVKPAGGSGDLVVGEHVVINAGC
VLYTGNGIHIGNYVAIAANCTFAPVNHAYADRSRLIREQGFLPSKGGIVIEDDVWIGANC
VLLDGAVLRRGCVVGAGSIVRGELLAYTVYAGQPLAAVGERR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory