SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ac3H11_1496 FitnessBrowser__acidovorax_3H11:Ac3H11_1496 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5gtlA NADPH complex structure of aldehyde dehydrogenase from bacillus cereus
46% identity, 97% coverage: 16:500/500 of query aligns to 12:490/491 of 5gtlA

query
sites
5gtlA

F

F

L

L

S

N

E

E

R

E

R

-

V

I

G

K

N

M

V

F

I

I

G

N

G

G

V

E

S

F

G

V

P

S

A

A

L

I

S

G

G

G

R

K

W

T

L

F

P

E

V

T

T

Y

D

N

P

P

A

A

T

T

E

E

M

D

V

V

V

L

A

A

E

V

A

V

P

C

D

E

S

A

D

Q

A

E

A

E

D

D

I

I

A

D

R

A

A

A

V

V

A

K

S

A

A

A

Q

R

R

S

A

A

F

F

D

E

S

S

H

G

V

P

W

W

R

A

G

E

L

M

R

T

P

T

A

A

D

E

R

R

E

A

K

H

L

L

L

I

F

Y

R

K

L

L

S

A

E

D

L

L

I

I

E

E

R

E

H

H

A

R

D

E

E

E

L

L

S

A

A

Q

L

L

E

E

T

A

L

L

Q

D

S

N

G

G

K

K

L

P

Q

Y

G

Q

I

V

A

A

R

L

A

D

I

D

D

D

V

I

Q

S

A

A

G

T

A

V

E

E

F

N

V

Y

R

R

Y

Y

M

Y

A

A

G

G

W

W

A

T

T

T

K

K

L

I

E

I

G

G

Q

Q

T

T

L

-

D

-

N

-

S

-

I

I

P

P

I

I

P

-

G

S

P

K

Q

D

W

Y

V

L

T

N

Y

Y

T

T

R

R

R

H

E

E

P

P

V

V

G

G

V

V

V

V

G

G

A

Q

I
|
I

V

I

P
|
P

W

W

N
|
N

F

F

P

P

L

L

A

V

I

M

A

S

L

S

W

W

K

K

I

M

A

G

P

A

A

A

L

L

A

A

A

T

G

G

C

C

T

T

V

I

V

V

L

L

K
|
K

P

P

S
x
A

E
|
E

D
x
Q

T

T

P

P

L

L

T

S

A

L

L

L

R

Y

L

A

A

A

H

K

L

L

A

F

L

K

E

E

A

A

G

G

I

F

P

P

E

N

G

G

V

V

L

V

N

N

V

F

V

V

C

P

G

G

R

F

G
|
G

A

P

T

E

A

A

G
|
G

A

A

A

A

L

I

I

V

A

N

H

H

P

H

G

D

V

I

R

D

K

K

L

V

S

A

F

F

T

T

G
|
G

S
|
S

T

T

A

V

V
x
T

G

G

K

K

V

Y

V

I

G

M

H

R

A

Q

A

S

V

A

E

E

N

M

M

I

A

K

R

H

F

V

T

T

L

L

E
|
E

L
|
L

G

G

G

G

K

K

S

S

P

P

A

N

V

I

V

I

M

L

E

E

D

D

A

A

D

D

P

L

S

E

Q

E

V

A

A

I

Q

N

G

G

I

A

A

F

T

Q

G

G

I

I

F

M

F

Y

H

N

Q

H

G

G

Q

Q

V

N

C
|
C

T

S

A

A

S

G

S

S

R

R

L

V

L

F

V

V

H

H

R

R

S

K

L

H

Y

Y

R

E

R

T

V

V

L

V

D

D

E

A

L

L

A

V

G

K

I

M

A

A

Q

N

G

N

M

V

R

K

I

L

G

G

S

A

G

G

F

M

D

E

A

K

A

E

T

T

Q

E

F

M

G

G

P

P

L

L

T

V

S

S

K

K

A

K

H

Q

F

Q

A

E

R

R

V

V

M

L

D

N

F

Y

I

I

A

E

S

Q

A

G

K

K

A

K

E

E

G

G

A

A

T

T

L

V

V

A

A

A

G

G

G

G

E

E

R

R

V

A

H

L

D

E

A

K

G

G

C

Y

F

F

V

V

Q

K

P

P

T

T

I

V

F

F

A

T

D

D

T

V

T

T

A

D

Q

D

M

M

R

T

V

I

V

V

R

K

E

E

E
|
E

V

I

F
|
F

G

G

P

P

V

V

L

V

A

V

V

V

A

L

P

P

F

F

D

D

D

S

V

T

E

E

D

E

A

V

I

I

A

E

A

R

A

A

N

N

D

N

T

S

P

S

Y

Y

G

G

L

L

A

A

A

A

S

G

L

V

W

W

T

T

Q

Q

S

N

L

I

S

K

H

T

A

G

H

H

R

Q

I

V

V

A

P

N

R

K

L

L

Q

K

A

A

G

G

V

T

V

V

W

W

V

I

N

N

A

D

H

Y

N

N

V

L

L

E

D

N

A

A

G

A

L

A

P

P

L

F

G

G

G

G

I

Y

K

K

Q

Q

S

S

G

G

T

I

G

G

R

R

D
x
E

L

L

G

G

R

S

A

Y

A

A

V

L

E

D

G

N

F

Y

T

T

E

E

L

V

K

K

S

S

V

V

C

W

M

V

A

N

V

I

active site: N165 (= N175), K188 (= K198), E263 (= E273), C297 (= C307), E394 (= E404), E471 (≠ D481)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I161 (= I171), P163 (= P173), K188 (= K198), A190 (≠ S200), E191 (= E201), Q192 (≠ D202), G221 (= G231), G225 (= G235), G241 (= G251), S242 (= S252), T245 (≠ V255), L264 (= L274), C297 (= C307), E394 (= E404), F396 (= F406)

5gtkA NAD+ complex structure of aldehyde dehydrogenase from bacillus cereus
46% identity, 97% coverage: 16:500/500 of query aligns to 12:490/491 of 5gtkA

query
sites
5gtkA

F

F

L

L

S

N

E

E

R

E

R

-

V

I

G

K

N

M

V

F

I

I

G

N

G

G

V

E

S

F

G

V

P

S

A

A

L

I

S

G

G

G

R

K

W

T

L

F

P

E

V

T

T

Y

D

N

P

P

A

A

T

T

E

E

M

D

V

V

V

L

A

A

E

V

A

V

P

C

D

E

S

A

D

Q

A

E

A

E

D

D

I

I

A

D

R

A

A

A

V

V

A

K

S

A

A

A

Q

R

R

S

A

A

F

F

D

E

S

S

H

G

V

P

W

W

R

A

G

E

L

M

R

T

P

T

A

A

D

E

R

R

E

A

K

H

L

L

L

I

F

Y

R

K

L

L

S

A

E

D

L

L

I

I

E

E

R

E

H

H

A

R

D

E

E

E

L

L

S

A

A

Q

L

L

E

E

T

A

L

L

Q

D

S

N

G

G

K

K

L

P

Q

Y

G

Q

I

V

A

A

R

L

A

D

I

D

D

D

V

I

Q

S

A

A

G

T

A

V

E

E

F

N

V

Y

R

R

Y

Y

M

Y

A

A

G

G

W

W

A

T

T

T

K

K

L

I

E

I

G

G

Q

Q

T

T

L

-

D

-

N

-

S

-

I

I

P

P

I

I

P

-

G

S

P

K

Q

D

W

Y

V

L

T

N

Y

Y

T

T

R

R

R

H

E

E

P

P

V

V

G

G

V

V

V

V

G

G

A

Q

I
|
I

V
x
I

P
|
P

W
|
W

N
|
N

F

F

P

P

L

L

A

V

I

M

A

S

L

S

W

W

K

K

I

M

A

G

P

A

A

A

L

L

A

A

A

T

G

G

C

C

T

T

V

I

V

V

L

L

K
|
K

P

P

S

A

E
|
E

D

Q

T

T

P

P

L

L

T

S

A

L

L

L

R

Y

L

A

A

A

H

K

L

L

A

F

L

K

E

E

A

A

G

G

I

F

P

P

E

N

G

G

V

V

L

V

N

N

V

F

V

V

C

P

G

G

R

F

G
|
G

A

P

T

E

A

A

G
|
G

A
|
A

A

A

L

I

I

V

A

N

H

H

P

H

G

D

V

I

R

D

K

K

L

V

S

A

F
|
F

T

T

G
|
G

S
|
S

T

T

A

V

V
x
T

G

G

K

K

V
x
Y

V

I

G

M

H

R

A

Q

A

S

V

A

E

E

N

M

M

I

A

K

R

H

F

V

T

T

L

L

E
|
E

L
|
L

G

G

G

G

K

K

S

S

P

P

A

N

V

I

V

I

M

L

E

E

D

D

A

A

D

D

P

L

S

E

Q

E

V

A

A

I

Q

N

G

G

I

A

A

F

T

Q

G

G

I

I

F

M

F

Y

H

N

Q

H

G

G

Q

Q

V

N

C
|
C

T

S

A

A

S

G

S

S

R

R

L

V

L

F

V

V

H

H

R

R

S

K

L

H

Y

Y

R

E

R

T

V

V

L

V

D

D

E

A

L

L

A

V

G

K

I

M

A

A

Q

N

G

N

M

V

R

K

I

L

G

G

S

A

G

G

F

M

D

E

A

K

A

E

T

T

Q

E

F

M

G

G

P

P

L

L

T

V

S

S

K

K

A

K

H
x
Q

F

Q

A

E

R
|
R

V

V

M

L

D

N

F

Y

I

I

A

E

S

Q

A

G

K

K

A

K

E

E

G

G

A

A

T

T

L

V

V

A

A

A

G

G

G

G

E

E

R

R

V

A

H

L

D

E

A

K

G

G

C

Y

F

F

V

V

Q

K

P

P

T

T

I

V

F

F

A

T

D

D

T

V

T

T

A

D

Q

D

M

M

R

T

V

I

V

V

R

K

E

E

E
|
E

V

I

F
|
F

G

G

P

P

V

V

L

V

A

V

V

V

A

L

P

P

F

F

D

D

D

S

V

T

E

E

D

E

A

V

I

I

A

E

A

R

A

A

N

N

D

N

T

S

P

S

Y

Y

G

G

L

L

A

A

A

A

S

G

L

V

W

W

T

T

Q

Q

S

N

L

I

S

K

H

T

A

G

H

H

R

Q

I

V

V

A

P

N

R

K

L

L

Q

K

A

A

G

G

V

T

V

V

W

W

V

I

N

N

A

D

H

Y

N

N

V

L

L

E

D

N

A

A

G

A

L

A

P

P

L

F

G

G

G

G

I

Y

K

K

Q

Q

S

S

G

G

T

I

G

G

R

R

D
x
E

L

L

G

G

R

S

A

Y

A

A

V

L

E

D

G

N

F

Y

T

T

E

E

L

V

K

K

S

S

V

V

C

W

M

V

A

N

V

I

active site: N165 (= N175), K188 (= K198), E263 (= E273), C297 (= C307), E394 (= E404), E471 (≠ D481)
binding nicotinamide-adenine-dinucleotide: I161 (= I171), I162 (≠ V172), P163 (= P173), W164 (= W174), K188 (= K198), E191 (= E201), G221 (= G231), G225 (= G235), A226 (= A236), F239 (= F249), G241 (= G251), S242 (= S252), T245 (≠ V255), Y248 (≠ V258), L264 (= L274), C297 (= C307), Q344 (≠ H354), R347 (= R357), E394 (= E404), F396 (= F406)

7um9A Human aldh1a1 with bound compound cm38 (see paper)
44% identity, 94% coverage: 33:500/500 of query aligns to 25:489/494 of 7um9A

query
sites
7um9A

A

S

L

V

S

S

G

G

R

K

W

K

L

F

P

P

V

V

T

F

D

N

P

P

A

A

T

T

E

E

M

E

V

E

V

L

A

C

E

Q

A

V

P

E

D

E

S

G

D

D

A

K

A

E

D

D

I

V

A

D

R

K

A

A

V

V

A

K

S

A

A

A

Q

R

R

Q

A

A

F

F

D

Q

-

I

S

G

H

S

V

P

W

W

R

R

G

T

L

M

R

D

P

A

A

S

D

E

R

R

E

G

K

R

L

L

L

L

F

Y

R

K

L

L

S

A

E

D

L

L

I

I

E

E

R

R

H

D

A

R

D

L

E

L

L

L

S

A

A

T

L

M

E

E

T

S

L

M

Q

N

S

G

G

G

K

K

L

L

Q

Y

G

S

I

N

A

A

R

Y

A

L

I

N

D

D

V

L

Q

A

A

G

G

C

A

I

E

K

F

T

V

L

R

R

Y

Y

M

C

A

A

G

G

W

W

A

A

T

D

K

K

L

I

E

Q

G

G

Q

R

T

T

L

-

D

-

N

-

S

-

I

I

P

P

I

I

P

D

G

G

P

-

Q

N

W

F

V

F

T

T

Y

Y

T

T

R

R

R

H

E

E

P

P

V

I

G

G

V

V

V

C

G

G

A

Q

I
|
I

V
x
I

P
|
P

W
|
W

N
|
N

F

F

P

P

L

L

A

V

I

M

A

L

L

I

W
|
W

K

K

I

I

A

G

P

P

A

A

L

L

A

S

A

C

G

G

C

N

T

T

V

V

V

V

L

V

K
|
K

P

P

S

A

E
|
E

D

Q

T

T

P

P

L

L

T

T

A

A

L

L

R

H

L

V

A

A

H

S

L

L

A

I

L

K

E

E

A

A

G

G

I

F

P

P

E

P

G

G

V

V

L

V

N

N

V

I

V

V

C

P

G

G

R

Y

G
|
G

A

P

T

T

A

A

G
|
G

A

A

A

A

L

I

I

S

A

S

H

H

P

M

G

D

V

I

R

D

K

K

L

V

S

A

F
|
F

T
|
T

G
|
G

S
|
S

T

T

A

E

V
|
V

G

G

K

K

V

L

V

I

G

K

H

E

A

A

A

A

-

G

V

K

E

S

N

N

M

L

A

K

R

R

F

V

T

T

L

L

E
|
E

L

L

G
|
G

G

G

K

K

S

S

P

P

A

C

V

I

V

V

M

L

E

A

D

D

A

A

D

D

P

L

S

D

Q

N

V

A

A

V

Q

E

G

F

I

A

A

H

T
x
H

G

G

I

V

F

F

F
x
Y

H

H

Q

Q

G

G

Q

Q

V

C

C

C

T
x
I

A

A

S

A

S

S

R

R

L

I

L

F

V

V

H

E

R

E

S

S

L

I

Y

Y

R

D

R

E

V

F

L

V

D

R

E

R

L

S

A

V

G

E

I

R

A

A

Q

K

G

K

M

Y

R

I

I

L

G

G

S

N

G

P

F

L

D

T

A

P

A

G

T

V

Q

T

F

Q

G

G

P

P

L

Q

T

I

S

D

K

K

A

E

H
x
Q

F

Y

A

D

R
x
K

V

I

M

L

D

D

F

L

I

I

A

E

S

S

A

G

K

K

A

K

E

E

G

G

A

A

T

K

L

L

V

E

A

C

G

G

G

G

E

G

R

P

V

W

H

G

D

N

A

K

G

G

C

Y

F

F

V

V

Q

Q

P

P

T

T

I

V

F

F

A

S

D

N

T

V

T

T

A

D

Q

E

M

M

R

R

V

I

V

A

R

K

E

E

E
|
E

V

I

F
|
F

G

G

P

P

V

V

L

Q

A

Q

V

I

A

M

P

K

F

F

D

K

D

S

V

L

E

D

D

D

A

V

I

I

A

K

A

R

A

A

N

N

D

N

T

T

P

F

Y

Y

G

G

L

L

A

S

A

A

S

G

L

V

W

F

T

T

Q

K

S

D

L

I

S

D

H

K

A

A

H

I

R

T

I

I

V

S

P

S

R

A

L

L

Q

Q

A

A

G

G

V

T

V

V

W

W

V

V

N

N

A

C

H

Y

N
x
G

V

V

L

V

D

S

A

A

G

Q

L

C

P

P

L

F

G

G

G

G

I

F

K

K

Q

M

S

S

G

G

T

N

G

G

R

R

D

E

L

L

G

G

R

E

A

Y

A

G

V

F

E

H

G

E

F

Y

T

T

E

E

L

V

K

K

S

T

V

V

C

T

M

V

A

K

V

I

binding nicotinamide-adenine-dinucleotide: I159 (= I171), I160 (≠ V172), P161 (= P173), W162 (= W174), N163 (= N175), K186 (= K198), E189 (= E201), G219 (= G231), G223 (= G235), F237 (= F249), T238 (= T250), G239 (= G251), S240 (= S252), V243 (= V255), E262 (= E273), G264 (= G275), Q343 (≠ H354), K346 (≠ R357), E393 (= E404), F395 (= F406)
binding (4-methylfuro[3,2-c]quinolin-2-yl)(piperidin-1-yl)methanone: W171 (= W183), H286 (≠ T297), Y290 (≠ F301), I297 (≠ T308), G451 (≠ N462)

5l2oA Crystal structure of aldh1a1 in complex with buc22 (see paper)
44% identity, 94% coverage: 33:500/500 of query aligns to 25:489/494 of 5l2oA

query
sites
5l2oA

A

S

L

V

S

S

G

G

R

K

W

K

L

F

P

P

V

V

T

F

D

N

P

P

A

A

T

T

E

E

M

E

V

E

V

L

A

C

E

Q

A

V

P

E

D

E

S

G

D

D

A

K

A

E

D

D

I

V

A

D

R

K

A

A

V

V

A

K

S

A

A

A

Q

R

R

Q

A

A

F

F

D

Q

-

I

S

G

H

S

V

P

W

W

R

R

G

T

L

M

R

D

P

A

A

S

D

E

R

R

E

G

K

R

L

L

L

L

F

Y

R

K

L

L

S

A

E

D

L

L

I

I

E

E

R

R

H

D

A

R

D

L

E

L

L

L

S

A

A

T

L

M

E

E

T

S

L

M

Q

N

S

G

G

G

K

K

L

L

Q

Y

G

S

I

N

A

A

R

Y

A

L

I

N

D

D

V

L

Q

A

A

G

G

C

A

I

E

K

F

T

V

L

R

R

Y

Y

M

C

A

A

G

G

W

W

A

A

T

D

K

K

L

I

E

Q

G

G

Q

R

T

T

L

-

D

-

N

-

S

-

I

I

P

P

I

I

P

D

G

G

P

-

Q

N

W

F

V

F

T

T

Y

Y

T

T

R

R

R

H

E

E

P

P

V

I

G

G

V

V

V

C

G

G

A

Q

I

I

V

I

P

P

W

W

N
|
N

F

F

P

P

L

L

A

V

I

M

A

L

L

I

W

W

K

K

I

I

A

G

P

P

A

A

L

L

A

S

A

C

G

G

C

N

T

T

V

V

V

V

L

V

K
|
K

P

P

S

A

E

E

D

Q

T

T

P

P

L

L

T

T

A

A

L

L

R

H

L

V

A

A

H

S

L

L

A

I

L

K

E

E

A

A

G

G

I

F

P

P

E

P

G

G

V

V

L

V

N

N

V

I

V

V

C

P

G

G

R

Y

G
|
G

A

P

T

T

A

A

G
|
G

A
|
A

A

A

L

I

I

S

A

S

H

H

P

M

G

D

V

I

R

D

K

K

L

V

S

A

F

F

T

T

G

G

S

S

T

T

A

E

V
|
V

G

G

K

K

V
x
L

V

I

G

K

H

E

A

A

A

A

-

G

V

K

E

S

N

N

M

L

A

K

R

R

F

V

T

T

L

L

E
|
E

L

L

G

G

G

G

K

K

S

S

P

P

A

C

V

I

V

V

M

L

E

A

D

D

A

A

D

D

P

L

S

D

Q

N

V

A

A

V

Q

E

G

F

I

A

A

H

T

H

G

G

I

V

F

F

F

Y

H

H

Q

Q

G

G

Q

Q

V

C

C
|
C

T

I

A

A

S

A

S

S

R

R

L

I

L

F

V

V

H

E

R

E

S

S

L

I

Y

Y

R

D

R

E

V

F

L

V

D

R

E

R

L

S

A

V

G

E

I

R

A

A

Q

K

G

K

M

Y

R

I

I

L

G

G

S

N

G

P

F

L

D

T

A

P

A

G

T

V

Q

T

F

Q

G

G

P

P

L

Q

T

I

S

D

K

K

A

E

H

Q

F

Y

A

D

R

K

V

I

M

L

D

D

F

L

I

I

A

E

S

S

A

G

K

K

A

K

E

E

G

G

A

A

T

K

L

L

V

E

A

C

G

G

G

G

E

G

R

P

V

W

H

G

D

N

A

K

G

G

C

Y

F

F

V

V

Q

Q

P

P

T

T

I

V

F

F

A

S

D

N

T

V

T

T

A

D

Q

E

M

M

R

R

V

I

V

A

R

K

E

E

E
|
E

V

I

F

F

G

G

P

P

V

V

L

Q

A

Q

V

I

A

M

P

K

F

F

D

K

D

S

V

L

E

D

D

D

A

V

I

I

A

K

A

R

A

A

N

N

D

N

T

T

P

F

Y

Y

G

G

L

L

A

S

A

A

S

G

L

V

W

F

T

T

Q

K

S

D

L

I

S

D

H

K

A

A

H

I

R

T

I

I

V

S

P

S

R

A

L

L

Q

Q

A

A

G

G

V

T

V

V

W

W

V

V

N

N

A

C

H

Y

N

G

V

V

L

V

D

S

A

A

G

Q

L

C

P

P

L

F

G

G

G

G

I

F

K

K

Q

M

S

S

G

G

T

N

G

G

R

R

D
x
E

L

L

G

G

R

E

A

Y

A

G

V

F

E

H

G

E

F

Y

T

T

E

E

L

V

K

K

S

T

V

V

C

T

M

V

A

K

V

I

active site: N163 (= N175), K186 (= K198), E262 (= E273), C296 (= C307), E393 (= E404), E470 (≠ D481)
binding 7-(diethylamino)-4-methyl-2H-1-benzopyran-2-one: G219 (= G231), G223 (= G235), A224 (= A236), V243 (= V255), L246 (≠ V258)

5l2nA Structure of aldh1a1 in complex with buc25 (see paper)
44% identity, 94% coverage: 33:500/500 of query aligns to 25:489/494 of 5l2nA

query
sites
5l2nA

A

S

L

V

S

S

G

G

R

K

W

K

L

F

P

P

V

V

T

F

D

N

P

P

A

A

T

T

E

E

M

E

V

E

V

L

A

C

E

Q

A

V

P

E

D

E

S

G

D

D

A

K

A

E

D

D

I

V

A

D

R

K

A

A

V

V

A

K

S

A

A

A

Q

R

R

Q

A

A

F

F

D

Q

-

I

S

G

H

S

V

P

W

W

R

R

G

T

L

M

R

D

P

A

A

S

D

E

R

R

E

G

K

R

L

L

L

L

F

Y

R

K

L

L

S

A

E

D

L

L

I

I

E

E

R

R

H

D

A

R

D

L

E

L

L

L

S

A

A

T

L

M

E

E

T

S

L

M

Q

N

S

G

G

G

K

K

L

L

Q

Y

G

S

I

N

A

A

R

Y

A

L

I

N

D

D

V

L

Q

A

A

G

G

C

A

I

E

K

F

T

V

L

R

R

Y

Y

M

C

A

A

G

G

W

W

A

A

T

D

K

K

L

I

E

Q

G

G

Q

R

T

T

L

-

D

-

N

-

S

-

I

I

P

P

I

I

P

D

G

G

P

-

Q

N

W

F

V

F

T

T

Y

Y

T

T

R

R

R

H

E

E

P

P

V

I

G

G

V

V

V

C

G

G

A

Q

I

I

V

I

P

P

W

W

N
|
N

F
|
F

P

P

L

L

A

V

I
x
M

A

L

L

I

W
|
W

K

K

I

I

A

G

P

P

A

A

L

L

A

S

A

C

G

G

C

N

T

T

V

V

V

V

L

V

K
|
K

P

P

S

A

E

E

D

Q

T

T

P

P

L

L

T

T

A

A

L

L

R

H

L

V

A

A

H

S

L

L

A

I

L

K

E

E

A

A

G

G

I

F

P

P

E

P

G

G

V

V

L

V

N

N

V

I

V

V

C

P

G

G

R

Y

G

G

A

P

T

T

A

A

G

G

A

A

A

A

L

I

I

S

A

S

H

H

P

M

G

D

V

I

R

D

K

K

L

V

S

A

F

F

T

T

G

G

S

S

T

T

A

E

V

V

G

G

K

K

V

L

V

I

G

K

H

E

A

A

A

A

-

G

V

K

E

S

N

N

M

L

A

K

R

R

F

V

T

T

L

L

E
|
E

L

L

G

G

G

G

K

K

S

S

P

P

A

C

V

I

V

V

M

L

E

A

D

D

A

A

D

D

P

L

S

D

Q

N

V

A

A

V

Q

E

G

F

I

A

A

H

T
x
H

G
|
G

I

V

F

F

F
x
Y

H

H

Q

Q

G

G

Q

Q

V
x
C

C
|
C

T
x
I

A

A

S

A

S

S

R

R

L

I

L

F

V

V

H

E

R

E

S

S

L

I

Y

Y

R

D

R

E

V

F

L

V

D

R

E

R

L

S

A

V

G

E

I

R

A

A

Q

K

G

K

M

Y

R

I

I

L

G

G

S

N

G

P

F

L

D

T

A

P

A

G

T

V

Q

T

F

Q

G

G

P

P

L

Q

T

I

S

D

K

K

A

E

H

Q

F

Y

A

D

R

K

V

I

M

L

D

D

F

L

I

I

A

E

S

S

A

G

K

K

A

K

E

E

G

G

A

A

T

K

L

L

V

E

A

C

G

G

G

G

E

G

R

P

V

W

H

G

D

N

A

K

G

G

C

Y

F

F

V

V

Q

Q

P

P

T

T

I

V

F

F

A

S

D

N

T

V

T

T

A

D

Q

E

M

M

R

R

V

I

V

A

R

K

E

E

E
|
E

V

I

F

F

G

G

P

P

V

V

L

Q

A

Q

V

I

A

M

P

K

F

F

D

K

D

S

V

L

E

D

D

D

A

V

I

I

A

K

A

R

A

A

N

N

D

N

T

T

P

F

Y

Y

G

G

L

L

A

S

A

A

S

G

L

V

W

F

T

T

Q

K

S

D

L

I

S

D

H

K

A

A

H

I

R

T

I

I

V

S

P

S

R

A

L

L

Q

Q

A

A

G

G

V

T

V

V

W

W

V

V

N

N

A

C

H
x
Y

N
x
G

V

V

L
x
V

D

S

A

A

G

Q

L

C

P

P

L
x
F

G

G

G

G

I

F

K

K

Q

M

S

S

G

G

T

N

G

G

R

R

D
x
E

L

L

G

G

R

E

A

Y

A

G

V

F

E

H

G

E

F

Y

T

T

E

E

L

V

K

K

S

T

V

V

C

T

M

V

A

K

V

I

active site: N163 (= N175), K186 (= K198), E262 (= E273), C296 (= C307), E393 (= E404), E470 (≠ D481)
binding 3-benzyl-4-methyl-2-oxo-2H-1-benzopyran-7-yl methanesulfonate: F164 (= F176), M168 (≠ I180), W171 (= W183), H286 (≠ T297), G287 (= G298), Y290 (≠ F301), C295 (≠ V306), C296 (= C307), I297 (≠ T308), Y450 (≠ H461), G451 (≠ N462), V453 (≠ L464), F459 (≠ L470)

5l2mA Structure of aldh1a1 in complex with buc11 (see paper)
44% identity, 94% coverage: 33:500/500 of query aligns to 25:489/494 of 5l2mA

query
sites
5l2mA

A

S

L

V

S

S

G

G

R

K

W

K

L

F

P

P

V

V

T

F

D

N

P

P

A

A

T

T

E

E

M

E

V

E

V

L

A

C

E

Q

A

V

P

E

D

E

S

G

D

D

A

K

A

E

D

D

I

V

A

D

R

K

A

A

V

V

A

K

S

A

A

A

Q

R

R

Q

A

A

F

F

D

Q

-

I

S

G

H

S

V

P

W

W

R

R

G

T

L

M

R

D

P

A

A

S

D

E

R

R

E

G

K

R

L

L

L

L

F

Y

R

K

L

L

S

A

E

D

L

L

I

I

E

E

R

R

H

D

A

R

D

L

E

L

L

L

S

A

A

T

L

M

E

E

T

S

L

M

Q

N

S

G

G

G

K

K

L

L

Q

Y

G

S

I

N

A

A

R

Y

A

L

I

N

D

D

V

L

Q

A

A

G

G

C

A

I

E

K

F

T

V

L

R

R

Y

Y

M

C

A

A

G

G

W

W

A

A

T

D

K

K

L

I

E

Q

G

G

Q

R

T

T

L

-

D

-

N

-

S

-

I

I

P

P

I

I

P

D

G

G

P

-

Q

N

W

F

V

F

T

T

Y

Y

T

T

R

R

R

H

E

E

P

P

V

I

G

G

V

V

V

C

G

G

A

Q

I

I

V

I

P

P

W

W

N
|
N

F
|
F

P

P

L

L

A

V

I

M

A

L

L

I

W

W

K

K

I

I

A

G

P

P

A

A

L

L

A

S

A

C

G

G

C

N

T

T

V

V

V

V

L

V

K
|
K

P

P

S

A

E

E

D

Q

T

T

P

P

L

L

T

T

A

A

L

L

R

H

L

V

A

A

H

S

L

L

A

I

L

K

E

E

A

A

G

G

I

F

P

P

E

P

G

G

V

V

L

V

N

N

V

I

V

V

C

P

G

G

R

Y

G

G

A

P

T

T

A

A

G

G

A

A

A

A

L

I

I

S

A

S

H

H

P

M

G

D

V

I

R

D

K

K

L

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S

A

F

F

T

T

G

G

S

S

T

T

A

E

V

V

G

G

K

K

V

L

V

I

G

K

H

E

A

A

A

A

-

G

V

K

E

S

N

N

M

L

A

K

R

R

F

V

T

T

L

L

E
|
E

L

L

G

G

G

G

K

K

S

S

P

P

A

C

V

I

V

V

M

L

E

A

D

D

A

A

D

D

P

L

S

D

Q

N

V

A

A

V

Q

E

G
x
F

I

A

A

H

T
x
H

G

G

I

V

F

F

F
x
Y

H

H

Q

Q

G

G

Q

Q

V

C

C
|
C

T

I

A

A

S

A

S

S

R

R

L

I

L

F

V

V

H

E

R

E

S

S

L

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Y

Y

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D

R

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V

F

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A

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G

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N

G

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F

L

D

T

A

P

A

G

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V

Q

T

F

Q

G

G

P

P

L

Q

T

I

S

D

K

K

A

E

H

Q

F

Y

A

D

R

K

V

I

M

L

D

D

F

L

I

I

A

E

S

S

A

G

K

K

A

K

E

E

G

G

A

A

T

K

L

L

V

E

A

C

G

G

G

G

E

G

R

P

V

W

H

G

D

N

A

K

G

G

C

Y

F

F

V

V

Q

Q

P

P

T

T

I

V

F

F

A

S

D

N

T

V

T

T

A

D

Q

E

M

M

R

R

V

I

V

A

R

K

E

E

E
|
E

V

I

F

F

G

G

P

P

V

V

L

Q

A

Q

V

I

A

M

P

K

F

F

D

K

D

S

V

L

E

D

D

D

A

V

I

I

A

K

A

R

A

A

N

N

D

N

T

T

P

F

Y

Y

G

G

L

L

A

S

A

A

S

G

L

V

W

F

T

T

Q

K

S

D

L

I

S

D

H

K

A

A

H

I

R

T

I

I

V

S

P

S

R

A

L

L

Q

Q

A

A

G

G

V

T

V

V

W

W

V

V

N

N

A

C

H

Y

N

G

V

V

L

V

D

S

A

A

G

Q

L

C

P

P

L

F

G

G

G

G

I

F

K

K

Q

M

S

S

G

G

T

N

G

G

R

R

D
x
E

L

L

G

G

R

E

A

Y

A

G

V

F

E

H

G

E

F

Y

T

T

E

E

L

V

K

K

S

T

V

V

C

T

M

V

A

K

V

I

active site: N163 (= N175), K186 (= K198), E262 (= E273), C296 (= C307), E393 (= E404), E470 (≠ D481)
binding 2,3,5-trimethyl-6-[3-oxo-3-(piperidin-1-yl)propyl]-7H-furo[3,2-g][1]benzopyran-7-one: F164 (= F176), F283 (≠ G294), H286 (≠ T297), Y290 (≠ F301)

4wpnA Structure of human aldh1a1 with inhibitor cm053 (see paper)
44% identity, 94% coverage: 33:500/500 of query aligns to 25:489/494 of 4wpnA

query
sites
4wpnA

A

S

L

V

S

S

G

G

R

K

W

K

L

F

P

P

V

V

T

F

D

N

P

P

A

A

T

T

E

E

M

E

V

E

V

L

A

C

E

Q

A

V

P

E

D

E

S

G

D

D

A

K

A

E

D

D

I

V

A

D

R

K

A

A

V

V

A

K

S

A

A

A

Q

R

R

Q

A

A

F

F

D

Q

-

I

S

G

H

S

V

P

W

W

R

R

G

T

L

M

R

D

P

A

A

S

D

E

R

R

E

G

K

R

L

L

L

L

F

Y

R

K

L

L

S

A

E

D

L

L

I

I

E

E

R

R

H

D

A

R

D

L

E

L

L

L

S

A

A

T

L

M

E

E

T

S

L

M

Q

N

S

G

G

G

K

K

L

L

Q

Y

G

S

I

N

A

A

R

Y

A

L

I

N

D

D

V

L

Q

A

A

G

G

C

A

I

E

K

F

T

V

L

R

R

Y

Y

M

C

A

A

G

G

W

W

A

A

T

D

K

K

L

I

E

Q

G

G

Q

R

T

T

L

-

D

-

N

-

S

-

I

I

P

P

I

I

P

D

G

G

P

-

Q

N

W

F

V

F

T

T

Y

Y

T

T

R

R

R

H

E

E

P

P

V

I

G

G

V

V

V

C

G

G

A

Q

I

I

V

I

P

P

W

W

N
|
N

F
|
F

P

P

L

L

A

V

I

M

A

L

L

I

W

W

K

K

I

I

A

G

P

P

A

A

L

L

A

S

A

C

G

G

C

N

T

T

V

V

V

V

L

V

K
|
K

P

P

S

A

E

E

D

Q

T

T

P

P

L

L

T

T

A

A

L

L

R

H

L

V

A

A

H

S

L

L

A

I

L

K

E

E

A

A

G

G

I

F

P

P

E

P

G

G

V

V

L

V

N

N

V

I

V

V

C

P

G

G

R

Y

G

G

A

P

T

T

A

A

G

G

A

A

A

A

L

I

I

S

A

S

H

H

P

M

G

D

V

I

R

D

K

K

L

V

S

A

F

F

T

T

G

G

S

S

T

T

A

E

V

V

G

G

K

K

V

L

V

I

G

K

H

E

A

A

A

A

-

G

V

K

E

S

N

N

M

L

A

K

R

R

F

V

T

T

L

L

E
|
E

L

L

G

G

G

G

K

K

S

S

P

P

A

C

V

I

V

V

M

L

E

A

D

D

A

A

D

D

P

L

S

D

Q

N

V

A

A

V

Q

E

G

F

I

A

A

H

T
x
H

G
|
G

I

V

F

F

F
x
Y

H

H

Q

Q

G

G

Q

Q

V
x
C

C
|
C

T
x
I

A

A

S

A

S

S

R

R

L

I

L

F

V

V

H

E

R

E

S

S

L

I

Y

Y

R

D

R

E

V

F

L

V

D

R

E

R

L

S

A

V

G

E

I

R

A

A

Q

K

G

K

M

Y

R

I

I

L

G

G

S

N

G

P

F

L

D

T

A

P

A

G

T

V

Q

T

F

Q

G

G

P

P

L

Q

T

I

S

D

K

K

A

E

H

Q

F

Y

A

D

R

K

V

I

M

L

D

D

F

L

I

I

A

E

S

S

A

G

K

K

A

K

E

E

G

G

A

A

T

K

L

L

V

E

A

C

G

G

G

G

E

G

R

P

V

W

H

G

D

N

A

K

G

G

C

Y

F

F

V

V

Q

Q

P

P

T

T

I

V

F

F

A

S

D

N

T

V

T

T

A

D

Q

E

M

M

R

R

V

I

V

A

R

K

E

E

E
|
E

V

I

F

F

G

G

P

P

V

V

L

Q

A

Q

V

I

A

M

P

K

F

F

D

K

D

S

V

L

E

D

D

D

A

V

I

I

A

K

A

R

A

A

N

N

D

N

T

T

P

F

Y

Y

G

G

L

L

A

S

A

A

S

G

L

V

W

F

T

T

Q

K

S

D

L

I

S

D

H

K

A

A

H

I

R

T

I

I

V

S

P

S

R

A

L

L

Q

Q

A

A

G

G

V

T

V

V

W

W

V

V

N

N

A

C

H

Y

N
x
G

V

V

L
x
V

D

S

A

A

G

Q

L

C

P

P

L

F

G

G

G

G

I

F

K

K

Q

M

S

S

G

G

T

N

G

G

R

R

D
x
E

L

L

G

G

R

E

A

Y

A

G

V

F

E

H

G

E

F

Y

T

T

E

E

L

V

K

K

S

T

V

V

C

T

M

V

A

K

V

I

active site: N163 (= N175), K186 (= K198), E262 (= E273), C296 (= C307), E393 (= E404), E470 (≠ D481)
binding 1-{[1,3-dimethyl-7-(3-methylbutyl)-2,6-dioxo-2,3,6,7-tetrahydro-1H-purin-8-yl]methyl}piperidine-4-carboxamide: F164 (= F176), H286 (≠ T297), G287 (= G298), Y290 (≠ F301), C295 (≠ V306), I297 (≠ T308), G451 (≠ N462), V453 (≠ L464)

7jwwA Crystal structure of human aldh1a1 bound to compound (r)-28 (see paper)
44% identity, 94% coverage: 33:500/500 of query aligns to 25:489/494 of 7jwwA

query
sites
7jwwA

A

S

L

V

S

S

G

G

R

K

W

K

L

F

P

P

V

V

T

F

D

N

P

P

A

A

T

T

E

E

M

E

V

E

V

L

A

C

E

Q

A

V

P

E

D

E

S

G

D

D

A

K

A

E

D

D

I

V

A

D

R

K

A

A

V

V

A

K

S

A

A

A

Q

R

R

Q

A

A

F

F

D

Q

-

I

S

G

H

S

V

P

W

W

R

R

G

T

L

M

R

D

P

A

A

S

D

E

R

R

E

G

K

R

L

L

L

L

F

Y

R

K

L

L

S

A

E

D

L

L

I

I

E

E

R

R

H

D

A

R

D

L

E

L

L

L

S

A

A

T

L

M

E

E

T

S

L

M

Q

N

S

G

G

G

K

K

L

L

Q

Y

G

S

I

N

A

A

R

Y

A

L

I

S

D

D

V

L

Q

A

A
x
G

G

C

A

I

E

K

F
x
T

V

L

R

R

Y

Y

M

C

A

A

G

G

W

W

A

A

T

D

K

K

L

I

E

Q

G

G

Q

R

T

T

L

-

D

-

N

-

S

-

I

I

P

P

I

I

P

D

G

G

P

-

Q

N

W

F

V

F

T

T

Y

Y

T

T

R

R

R

H

E

E

P

P

V

I

G

G

V

V

V

C

G

G

A

Q

I

I

V

I

P

P

W

W

N
|
N

F
|
F

P

P

L

L

A

V

I
x
M

A

L

L

I

W

W

K

K

I

I

A

G

P

P

A

A

L

L

A

S

A

C

G

G

C

N

T

T

V

V

V

V

L

V

K
|
K

P

P

S

A

E

E

D

Q

T

T

P

P

L

L

T

T

A

A

L

L

R

H

L

V

A

A

H

S

L

L

A

I

L

K

E

E

A

A

G

G

I

F

P

P

E

P

G

G

V

V

L

V

N

N

V

I

V

V

C

P

G

G

R

Y

G

G

A

P

T

T

A

A

G

G

A

A

A

A

L

I

I

S

A

S

H

H

P

M

G

D

V

I

R

D

K

K

L

V

S

A

F

F

T

T

G

G

S

S

T

T

A

E

V

V

G

G

K

K

V

L

V

I

G

K

H

E

A

A

A

A

-

G

V

K

E

S

N

N

M

L

A

K

R

R

F

V

T

T

L

L

E
|
E

L

L

G

G

G

G

K

K

S

S

P

P

A

C

V

I

V

V

M

L

E

A

D

D

A

A

D

D

P

L

S

D

Q

N

V

A

A

V

Q

E

G

F

I

A

A

H

T

H

G

G

I

V

F

F

F
x
Y

H

H

Q

Q

G

G

Q

Q

V
x
C

C
|
C

T
x
I

A

A

S

A

S

S

R

R

L

I

L

F

V

V

H

E

R

E

S

S

L

I

Y

Y

R

D

R

E

V

F

L

V

D

R

E

R

L

S

A

V

G

E

I

R

A

A

Q

K

G

K

M

Y

R

I

I

L

G

G

S

N

G

P

F

L

D

T

A

P

A

G

T

V

Q

T

F

Q

G

G

P

P

L

Q

T

I

S

D

K

K

A

E

H

Q

F

Y

A

D

R

K

V

I

M

L

D

D

F

L

I

I

A

E

S

S

A

G

K

K

A

K

E

E

G

G

A

A

T

K

L

L

V

E

A

C

G

G

G

G

E

G

R

P

V

W

H

G

D

N

A

K

G

G

C

Y

F

F

V

V

Q

Q

P

P

T

T

I

V

F

F

A

S

D

N

T

V

T

T

A

D

Q

E

M

M

R

R

V

I

V

A

R

K

E

E

E
|
E

V

I

F

F

G

G

P

P

V

V

L

Q

A

Q

V

I

A

M

P

K

F

F

D

K

D

S

V

L

E

D

D

D

A

V

I

I

A

K

A

R

A

A

N

N

D

N

T

T

P

F

Y

Y

G

G

L

L

A

S

A

A

S

G

L

V

W

F

T

T

Q

K

S

D

L

I

S

D

H

K

A

A

H

I

R

T

I

I

V

S

P

S

R

A

L

L

Q

Q

A

A

G

G

V

T

V

V

W

W

V

V

N

N

A

C

H

Y

N

G

V

V

L
x
V

D

S

A

A

G

Q

L

C

P

P

L
x
F

G

G

G

G

I

F

K

K

Q

M

S

S

G

G

T

N

G

G

R

R

D
x
E

L

L

G

G

R

E

A

Y

A

G

V

F

E

H

G

E

F

Y

T

T

E

E

L

V

K

K

S

T

V

V

C

T

M

V

A

K

V

I

active site: N163 (= N175), K186 (= K198), E262 (= E273), C296 (= C307), E393 (= E404), E470 (≠ D481)
binding 5-{4-[(Z)-2-hydroxyethenyl]phenyl}-1-methyl-6-{[(1R)-1-phenylethyl]sulfanyl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one: G118 (≠ A125), T122 (≠ F129), F164 (= F176), M168 (≠ I180), Y290 (≠ F301), C295 (≠ V306), C296 (= C307), I297 (≠ T308), V453 (≠ L464), F459 (≠ L470)

7jwvA Crystal structure of human aldh1a1 bound to compound (r)-28 (see paper)
44% identity, 94% coverage: 33:500/500 of query aligns to 25:489/494 of 7jwvA

query
sites
7jwvA

A

S

L

V

S

S

G

G

R

K

W

K

L

F

P

P

V

V

T

F

D

N

P

P

A

A

T

T

E

E

M

E

V

E

V

L

A

C

E

Q

A

V

P

E

D

E

S

G

D

D

A

K

A

E

D

D

I

V

A

D

R

K

A

A

V

V

A

K

S

A

A

A

Q

R

R

Q

A

A

F

F

D

Q

-

I

S

G

H

S

V

P

W

W

R

R

G

T

L

M

R

D

P

A

A

S

D

E

R

R

E

G

K

R

L

L

L

L

F

Y

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K

L

L

S

A

E

D

L

L

I

I

E

E

R

R

H

D

A

R

D

L

E

L

L

L

S

A

A

T

L

M

E

E

T

S

L

M

Q

N

S

G

G

G

K

K

L

L

Q

Y

G

S

I

N

A

A

R

Y

A

L

I

S

D

D

V

L

Q

A

A
x
G

G

C

A

I

E

K

F
x
T

V

L

R

R

Y

Y

M

C

A

A

G

G

W

W

A

A

T

D

K

K

L

I

E

Q

G

G

Q

R

T

T

L

-

D

-

N

-

S

-

I

I

P

P

I

I

P

D

G

G

P

-

Q

N

W

F

V

F

T

T

Y

Y

T

T

R

R

R

H

E

E

P

P

V

I

G

G

V

V

V

C

G

G

A

Q

I

I

V

I

P

P

W

W

N
|
N

F
|
F

P

P

L

L

A

V

I
x
M

A

L

L

I

W

W

K

K

I

I

A

G

P

P

A

A

L

L

A

S

A

C

G

G

C

N

T

T

V

V

V

V

L

V

K
|
K

P

P

S

A

E

E

D

Q

T

T

P

P

L

L

T

T

A

A

L

L

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H

L

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A

A

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L

L

A

I

L

K

E

E

A

A

G

G

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F

P

P

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G

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V

L

V

N

N

V

I

V

V

C

P

G

G

R

Y

G

G

A

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T

T

A

A

G

G

A

A

A

A

L

I

I

S

A

S

H

H

P

M

G

D

V

I

R

D

K

K

L

V

S

A

F

F

T

T

G

G

S

S

T

T

A

E

V

V

G

G

K

K

V

L

V

I

G

K

H

E

A

A

A

A

-

G

V

K

E

S

N

N

M

L

A

K

R

R

F

V

T

T

L

L

E
|
E

L

L

G

G

G

G

K

K

S

S

P

P

A

C

V

I

V

V

M

L

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A

D

D

A

A

D

D

P

L

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Q

N

V

A

A

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Q

E

G

F

I

A

A

H

T

H

G

G

I

V

F

F

F
x
Y

H

H

Q

Q

G

G

Q

Q

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x
C

C
|
C

T
x
I

A

A

S

A

S

S

R

R

L

I

L

F

V

V

H

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Y

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K

K

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L

D

D

F

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A

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A

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K

A

K

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G

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L

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G

G

G

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W

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A

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G

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Y

F

F

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V

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N

T

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T

A

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M

M

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A

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|
E

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F

F

G

G

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P

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A

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A

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K

F

F

D

K

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V

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D

D

A

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I

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A

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A

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A

A

N

N

D

N

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F

Y

Y

G

G

L

L

A

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A

A

S

G

L

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W

F

T

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Q

K

S

D

L

I

S

D

H

K

A

A

H

I

R

T

I

I

V

S

P

S

R

A

L

L

Q

Q

A

A

G

G

V

T

V

V

W

W

V

V

N

N

A

C

H

Y

N

G

V

V

L
x
V

D

S

A

A

G

Q

L

C

P

P

L
x
F

G

G

G

G

I

F

K

K

Q

M

S

S

G

G

T

N

G

G

R

R

D
x
E

L

L

G

G

R

E

A

Y

A

G

V

F

E

H

G

E

F

Y

T

T

E

E

L

V

K

K

S

T

V

V

C

T

M

V

A

K

V

I

active site: N163 (= N175), K186 (= K198), E262 (= E273), C296 (= C307), E393 (= E404), E470 (≠ D481)
binding 5-[4-(hydroxymethyl)phenyl]-1-methyl-6-{[(1R)-1-phenylethyl]sulfanyl}-1,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one: G118 (≠ A125), T122 (≠ F129), F164 (= F176), M168 (≠ I180), Y290 (≠ F301), C295 (≠ V306), I297 (≠ T308), V453 (≠ L464), F459 (≠ L470)

7jwuA Crystal structure of human aldh1a1 bound to compound (r)-28 (see paper)
44% identity, 94% coverage: 33:500/500 of query aligns to 25:489/494 of 7jwuA

query
sites
7jwuA

A

S

L

V

S

S

G

G

R

K

W

K

L

F

P

P

V

V

T

F

D

N

P

P

A

A

T

T

E

E

M

E

V

E

V

L

A

C

E

Q

A

V

P

E

D

E

S

G

D

D

A

K

A

E

D

D

I

V

A

D

R

K

A

A

V

V

A

K

S

A

A

A

Q

R

R

Q

A

A

F

F

D

Q

-

I

S

G

H

S

V

P

W

W

R

R

G

T

L

M

R

D

P

A

A

S

D

E

R

R

E

G

K

R

L

L

L

L

F

Y

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K

L

L

S

A

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D

L

L

I

I

E

E

R

R

H

D

A

R

D

L

E

L

L

L

S

A

A

T

L

M

E

E

T

S

L

M

Q

N

S

G

G

G

K

K

L

L

Q

Y

G

S

I

N

A

A

R

Y

A

L

I

S

D

D

V

L

Q

A

A

G

G

C

A

I

E

K

F
x
T

V

L

R

R

Y

Y

M

C

A

A

G

G

W

W

A

A

T

D

K

K

L

I

E

Q

G

G

Q

R

T

T

L

-

D

-

N

-

S

-

I

I

P

P

I

I

P

D

G

G

P

-

Q

N

W

F

V

F

T

T

Y

Y

T

T

R

R

R

H

E

E

P

P

V

I

G

G

V

V

V

C

G

G

A

Q

I
|
I

V
x
I

P
|
P

W
|
W

N
|
N

F
|
F

P

P

L

L

A

V

I

M

A

L

L

I

W
|
W

K

K

I

I

A

G

P

P

A

A

L

L

A

S

A

C

G

G

C

N

T

T

V

V

V

V

L

V

K
|
K

P

P

S

A

E
|
E

D

Q

T

T

P

P

L

L

T

T

A

A

L

L

R

H

L

V

A

A

H
&