SitesBLAST

Comparing Ac3H11_1528 FitnessBrowser__acidovorax_3H11:Ac3H11_1528 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Found 20 (the maximum) hits to proteins with known functional sites

4xxvA Crystal structure of 3-isopropylmalate dehydrogenase from burkholderia thailandensis in complex with NAD
79% identity, 99% coverage: 1:355/357 of query aligns to 2:356/356 of 4xxvA

• active site: Y136 (= Y135), K191 (= K190), D223 (= D222), D247 (= D246), D251 (= D250)
• binding nicotinamide-adenine-dinucleotide: N193 (= N192), Y221 (= Y220), N224 (= N223)

4iwhA Crystal structure of a 3-isopropylmalate dehydrogenase from burkholderia pseudomallei
79% identity, 99% coverage: 1:355/357 of query aligns to 4:358/358 of 4iwhA

• active site: Y138 (= Y135), K193 (= K190), D225 (= D222), D249 (= D246), D253 (= D250)
• binding magnesium ion: D249 (= D246), D253 (= D250)

1a05A Crystal structure of the complex of 3-isopropylmalate dehydrogenase from thiobacillus ferrooxidans with 3-isopropylmalate (see paper)
61% identity, 99% coverage: 2:355/357 of query aligns to 3:354/357 of 1a05A

• active site: Y140 (= Y135), K190 (= K190), D222 (= D222), D246 (= D246), D250 (= D250)
• binding 3-isopropylmalic acid: R95 (= R90), R105 (= R100), R133 (= R128), Y140 (= Y135), D246 (= D246)

Q56268 3-isopropylmalate dehydrogenase; 3-IPM-DH; Beta-IPM dehydrogenase; IMDH; EC 1.1.1.85 from Acidithiobacillus ferrooxidans (Thiobacillus ferrooxidans) (see paper)
61% identity, 99% coverage: 2:355/357 of query aligns to 3:354/358 of Q56268

• R95 (= R90) binding substrate
• R105 (= R100) binding substrate
• R133 (= R128) binding substrate
• D222 (= D222) binding Mg(2+); binding substrate
• D246 (= D246) binding Mg(2+)

P93832 3-isopropylmalate dehydrogenase 2, chloroplastic; 3-IPM-DH 2; AtIMDH2; AtIMDH3; IMDH 2; Beta-IPM dehydrogenase 2; Isopropylmalate dehydrogenase 2; AtIMD2; EC 1.1.1.85 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
57% identity, 99% coverage: 3:355/357 of query aligns to 45:397/405 of P93832

• 114:129 (vs. 68:83, 44% identical) binding NAD(+)
• L132 (≠ I86) mutation to A: Reduced activity toward 3-isopropylmalate.
• L133 (= L87) Confers substrate specificity; mutation to A: Reduced activity toward 3-isopropylmalate.; mutation to F: Enhanced activity toward 3-(2'-methylthio)-ethylmalate, but reduced catalytic efficiency with 3-isopropylmalate.
• R136 (= R90) binding substrate; mutation to A: Loss of activity toward 3-isopropylmalate.; mutation to K: Reduced activity toward 3-isopropylmalate.
• R146 (= R100) binding substrate; mutation to A: Reduced activity toward 3-isopropylmalate.; mutation to K: Reduced activity toward 3-isopropylmalate.
• R174 (= R128) binding substrate; mutation to A: Loss of activity toward 3-isopropylmalate.; mutation to K: Reduced activity toward 3-isopropylmalate.
• Y181 (= Y135) Important for catalysis; mutation Y->A,F,H: Reduced activity toward 3-isopropylmalate.
• K232 (= K190) Important for catalysis; mutation to M: Loss of activity toward 3-isopropylmalate.
• N234 (= N192) binding NAD(+); mutation N->A,D: Loss of activity toward 3-isopropylmalate.
• V235 (= V193) mutation to A: Reduced activity toward 3-isopropylmalate.
• D264 (= D222) binding Mg(2+); binding substrate; mutation to N: Loss of activity toward 3-isopropylmalate.
• N265 (= N223) binding NAD(+)
• D288 (= D246) binding Mg(2+); mutation to N: Loss of activity toward 3-isopropylmalate.
• D292 (= D250) binding Mg(2+); mutation to N: Reduced activity toward 3-isopropylmalate.
• 318:334 (vs. 276:292, 76% identical) binding NAD(+)

5j33A Isopropylmalate dehydrogenase in complex with NAD+ (see paper)
57% identity, 99% coverage: 3:355/357 of query aligns to 5:357/360 of 5j33A

• active site: Y141 (= Y135), K192 (= K190), D224 (= D222), D248 (= D246), D252 (= D250)
• binding magnesium ion: D248 (= D246), D252 (= D250)
• binding nicotinamide-adenine-dinucleotide: I74 (≠ V68), E89 (= E83), L92 (≠ I86), I261 (= I259), E278 (= E276), H281 (= H279), G282 (= G280), S283 (= S281), A284 (= A282), I287 (= I285), N294 (= N292), D335 (= D333)

5j32A Isopropylmalate dehydrogenase in complex with isopropylmalate (see paper)
57% identity, 99% coverage: 3:355/357 of query aligns to 15:367/369 of 5j32A

• active site: L18 (= L6), Y151 (= Y135), K202 (= K190), D234 (= D222), D258 (= D246), D262 (= D250)
• binding 3-isopropylmalic acid: R106 (= R90), R144 (= R128), Y151 (= Y135), D258 (= D246)
• binding magnesium ion: D258 (= D246), D262 (= D250)

Q9FMT1 3-isopropylmalate dehydrogenase 1, chloroplastic; 3-IPM-DH 1; AtIMDH1; IMDH 1; Beta-IPM dehydrogenase 1; Isopropylmalate dehydrogenase 1; AtIMD1; Methylthioalkylmalate dehydrogenase 1; EC 1.1.1.85 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
57% identity, 99% coverage: 2:355/357 of query aligns to 48:401/409 of Q9FMT1

• F137 (≠ L87) Confers substrate specificity; mutation to L: Reduced activity toward 3-(2'-methylthio)-ethylmalate, but enhanced catalytic efficiency with 3-isopropylmalate.
• C232 (≠ T186) Essential for redox regulation; mutation to S: Reduced sensitivity to oxidation on enzyme activity regulation.
• C390 (≠ T344) Essential for redox regulation; mutation to S: Reduced sensitivity to oxidation on enzyme activity regulation.

Q9SA14 3-isopropylmalate dehydrogenase 3, chloroplastic; 3-IPM-DH 3; AtIMDH2; AtIMDH3; IMDH 3; Beta-IPM dehydrogenase 3; Isopropylmalate dehydrogenase 3; AtIMD3; EC 1.1.1.85 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
56% identity, 99% coverage: 2:355/357 of query aligns to 45:398/404 of Q9SA14

• L134 (= L87) Confers substrate specificity; mutation to F: Enhanced activity toward 3-(2'-methylthio)-ethylmalate, but reduced catalytic efficiency with 3-isopropylmalate.

6xxyA Crystal structure of haemophilus influenzae 3-isopropylmalate dehydrogenase in complex with o-isobutenyl oxalylhydroxamate. (see paper)
56% identity, 99% coverage: 2:355/357 of query aligns to 5:358/358 of 6xxyA

• active site: Y144 (= Y135), K194 (= K190), D226 (= D222), D250 (= D246)
• binding magnesium ion: D250 (= D246), D254 (= D250)
• binding nicotinamide-adenine-dinucleotide: S74 (≠ A67), V75 (= V68), G76 (= G69), E90 (= E83), L94 (≠ I86), Y224 (= Y220), N227 (= N223), M230 (= M226), M263 (≠ I259), G264 (= G260), E280 (= E276), G283 (≠ H279), G284 (= G280), S285 (= S281), A286 (= A282), P287 (= P283), D288 (= D284), I289 (= I285), N296 (= N292), D337 (= D333)
• binding 2-(2-methylprop-2-enoxyamino)-2-oxidanylidene-ethanoic acid: E90 (= E83), R108 (= R100), R137 (= R128), K194 (= K190), V197 (= V193), D226 (= D222), D250 (= D246)

1cnzA 3-isopropylmalate dehydrogenase (ipmdh) from salmonella typhimurium (see paper)
54% identity, 99% coverage: 3:356/357 of query aligns to 7:360/363 of 1cnzA

• active site: Y145 (= Y135), K195 (= K190), D227 (= D222), D251 (= D246)
• binding manganese (ii) ion: D251 (= D246), D255 (= D250)

P37412 3-isopropylmalate dehydrogenase; 3-IPM-DH; Beta-IPM dehydrogenase; IMDH; EC 1.1.1.85 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
54% identity, 99% coverage: 3:356/357 of query aligns to 7:360/363 of P37412

• D227 (= D222) binding Mn(2+)
• D251 (= D246) binding Mn(2+)
• D255 (= D250) binding Mn(2+)

3vkzA 3-isopropylmalate dehydrogenase from shewanella oneidensis mr-1 at atmospheric pressure (see paper)
55% identity, 100% coverage: 2:357/357 of query aligns to 4:362/364 of 3vkzA