SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ac3H11_1849 FitnessBrowser__acidovorax_3H11:Ac3H11_1849 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1cjxA Crystal structure of pseudomonas fluorescens hppd (see paper)
74% identity, 93% coverage: 27:379/381 of query aligns to 1:351/352 of 1cjxA

query
sites
1cjxA

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binding acetate ion: H237 (= H263), F334 (= F362)
binding fe (ii) ion: H158 (= H184), H237 (= H263), E319 (= E345)

7xntA Crystal structure of pfhppd-y13161 complex
73% identity, 91% coverage: 27:373/381 of query aligns to 4:341/341 of 7xntA

query
sites
7xntA

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binding 3-(2,6-dimethylphenyl)-1-methyl-6-(2-oxidanyl-6-oxidanylidene-cyclohexen-1-yl)carbonyl-quinazoline-2,4-dione: H161 (= H184), T163 (= T186), S201 (= S224), P214 (= P237), Q221 (= Q248), H236 (= H263), F304 (= F334), F313 (= F343), F325 (= F357), F330 (= F362)
binding cobalt (ii) ion: H161 (= H184), H236 (= H263), E315 (= E345)

7x8eA Crystal structure of pfhppd-y13287 complex
72% identity, 92% coverage: 27:377/381 of query aligns to 3:341/341 of 7x8eA

query
sites
7x8eA

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binding 1,5-dimethyl-3-(2-methylphenyl)-6-(2-oxidanyl-6-oxidanylidene-cyclohexen-1-yl)carbonyl-quinazoline-2,4-dione: S193 (= S224), P206 (= P237), Q214 (= Q248), H229 (= H263), M252 (≠ A286), F300 (= F334), F321 (= F357), F326 (= F362)
binding cobalt (ii) ion: H160 (= H184), H229 (= H263), E311 (= E345)

7xntC Crystal structure of pfhppd-y13161 complex
69% identity, 90% coverage: 27:368/381 of query aligns to 3:320/320 of 7xntC

query
sites
7xntC

W

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E

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M

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-

I

-

Q

-

G

-

E

-

Y

-

T

-

G

-

L

-

T

T

S

S

K

K

A

A

M

M

T

S

A

A

P

P

D

D

G

G

K

M

I

I

R

R

I

I

P
|
P

L

L

N

N

E

E

E

E

S

-

K

-

Q

-

G

-

G

-

G

-

Q
|
Q

I

I

E

E

E

E

F

F

L

L

M

M

Q

Q

F

F

N

N

G

G

E

E

G

G

I

I

Q

Q

H
|
H

I

V

A

A

L

F

I

L

C

T

D

D

N

D

L

L

L

V

D

K

V

T

V

W

D

D

K

A

L

L

G

K

M

K

A

I

G

G

V

M

Q

R

L

F

A
x
M

T

T

A

A

P

P

N

P

E

D

V

T

Y

Y

Y

Y

E

E

M

M

L

L

D

E

T

G

R

R

L

L

P

P

G

D

H

H

G

G

Q

E

P

P

V

V

P

D

E

Q

L

L

Q

Q

S

A

R

R

G

G

I

I

L

L

L

L

D

D

G

G

T

S

T

S

A

K

D

-

G

-

T

-

P

-

R

R

L

L

L

L

L

L

Q

Q

I

I

F
|
F

S

S

T

E

P

T

M

L

L

M

G

G

P

P

V

V

F

F

F

F

E
|
E

F

F

I

I

Q

Q

R

R

E

K

G

G

D

D

Y

-

R

-

D

D

G

G

F
|
F

G
|
G

E

E

G

G

N
|
N

F
|
F

K

K

A

A

L

L

F

F

E

E

S

S

binding 3-(2,6-dimethylphenyl)-1-methyl-6-(2-oxidanyl-6-oxidanylidene-cyclohexen-1-yl)carbonyl-quinazoline-2,4-dione: H160 (= H184), T162 (= T186), P201 (= P237), Q206 (= Q248), H221 (= H263), M244 (≠ A286), F288 (= F334), F309 (= F357), G310 (= G358), N313 (= N361), F314 (= F362)
binding cobalt (ii) ion: H160 (= H184), H221 (= H263), E299 (= E345)

Q88JU3 3-dehydroshikimate dehydratase; DSD; EC 4.2.1.118 from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
33% identity, 88% coverage: 35:371/381 of query aligns to 295:624/635 of Q88JU3

query
sites
Q88JU3

G

G

F

V

E

E

F

F

V

L

E

E

F

F

T

A

S

V

P

D

Q

E

P

A

-

V

-

G

G

A

V

R

L

L

E

G

A

N

V

W

F

L

E

K

K

R

L

L

G

G

F

F

T

A

L

E

V

A

A

G

K

K

H

H

R

R

S

S

K

K

D

E

V

V

V

Q

L

L

Y

L

R

R

Q

Q

N

G

G

D

I

I

N

N

F

I

I

V

L

L

N

N

R

A

E

E

P

P

H

Y

S

S

Q

F

A

G

A

H

Y

N

F

F

G

F

A

E

E

A

H

H

G

G

P

P

S

S

A

L

C

C

G

A

L

T

A

A

F

L

R

R

V

V

K

K

D

D

A

Q

H

Q

K

A

A

A

Y

L

N

K

R

R

A

A

L

T

E

A

L

F

G

R

A

G

Q

Q

P

P

I

F

E

R

I

G

P

L

T

V

G

G

P

P

M

N

E

E

L

C

R

E

L

V

P

P

A

A

I

V

K

R

G

A

I

P

G

D

G

G

A

S

P

L

L

L

Y

Y

L

L

I

V

D

E

R

Q

F

G

E

T

D

A

G

G

K

H

S

T

I

L

Y

Y

D

D

I

T

D

D

F

F

E

S

F

L

I

D

E

N

G

N

V

A

D

-

R

-

R

-

P

T

A

A

G

T

H

G

G

G

L

L

N

R

L

R

I

I

D

D

H
|
H

L

M

T

A

H

L

N

A

V

L

Y

P

R

A

G

E

R

S

M

L

G

D

F

S

W

W

A

V

N

L

F

F

Y

Y

E

K

K

S

L

L

F

F

G

D

F

F

R

A

E

A

I

D

R

D

Y

E

F

V

D

V

I

L

Q

P

G

D

E

P

Y

Y

T

G

G

L

L

V

T

K

S

S

K

R

A

A

M

L

T

R

A

S

P

Q

D

C

G

G

K

T

I

L

R

R

I

L

P

P

L

L

N

N

E

-

E

I

S

S

K

E

Q

N

G

R

G

N

G

T

Q

A

I

I

E

A

E

H

F

A

L

L

M

S

Q

S

F

Y

N

R

G

G

E

S

G

G

I

V

Q

H

H
|
H

I

I

A

A

L

F

I

D

C

C

D

D

N

D

L

I

L

F

D

R

V

E

V

V

D

A

K

R

L

A

G

K

M

L

A

A

G

G

V

V

Q

P

L

L

A

L

T

E

A

I

P

P

N

L

E

N

V

-

Y

Y

Y

Y

E

D

M

D

L

L

D

A

T

A

R

R

L

F

P

D

G

F

H

D

G

D

Q

E

P

F

V

L

P

S

E

E

L

L

Q

A

S

Y

R

Y

G

N

I

V

L

L

L

Y

D

D

G

R

T

D

T

A

A

Q

D

G

G

G

T

E

P

-

R

-

L

-

L

L

L

F

Q

H

I

V

F

Y

S

T

T

E

P

P

M

F

L

E

G

E

P

R

V

F

F

F

F

F

E
|
E

F

I

I

I

Q

Q

R

R

E

K

G

A

D

G

Y

Y

R

A

D

-

G

G

F

Y

G

G

E

A

G

A

N

N

F

V

K

A

A

V

L

R

F

L

E

A

S

A

L

M

E

A

R

K

H443 (= H184) binding
H521 (= H263) binding
E599 (= E345) binding

Sites not aligning to the query:

134 binding
165 binding
168 H→A: 21-fold decrease in turnover rate.
191 binding
206 S→A: 10-fold decrease in the affinity for dehydroshikimate without significantly altering the turnover rate.
239 binding

5hmqD Xylose isomerase-like tim barrel/4-hydroxyphenylpyruvate dioxygenase fusion protein
33% identity, 88% coverage: 35:371/381 of query aligns to 297:621/624 of 5hmqD

query
sites
5hmqD

G

G

F

V

E

E

F

F

V

L

E

E

F

F

T

A

S

V

P

D

Q

E

P

A

-

V

-

G

G

A

V

R

L

L

E

G

A

N

V

W

F

L

E

K

K

R

L

L

G

G

F

F

T

A

L

E

V

A

A

G

K

K

H

H

R

R

S

S

K

K

D

E

V

V

V

Q

L

L

Y

L

R

R

Q

Q

N

G

G

D

I

I

N

N

F

I

I

V

L

L

N

N

R

A

E

E

P

P

H

Y

S

S

Q

F

A

G

A

H

Y

N

F

F

G

F

A

E

E

A

H

H

G

G

P

P

S

S

A

L

C

C

G

A

L

T

A

A

F

L

R

R

V

V

K

K

D

D

A

Q

H

Q

K

A

A

A

Y

L

N

K

R

R

A

A

L

T

E

A

L

F

G

R

A

G

Q

Q

P

P

I

F

E

R

I

G

P

L

T

V

G

G

P

P

M

N

E

E

L

C

R

E

L

V

P

P

A

A

I

V

K

R

G

A

I

P

G

D

G

G

A

S

P

L

L

L

Y

Y

L

L

I

V

D

E

R

-

F

-

E

Q

D

G

G

T

K

H

S

T

I

L

Y

Y

D

D

I

T

D

D

F

F

E

S

F

L

I

D

E

N

G

N

V

A

D

-

R

-

R

-

P

T

A

A

G

T

H

G

G

G

L

L

N

R

L

R

I

I

D

D

H
|
H

L

M

T

A

H

L

N

A

V

L

Y

P

R

A

G

E

R

S

M

L

G

D

F

S

W

W

A

V

N

L

F

F

Y

Y

E

K

K

S

L

L

F

F

G

D

F

F

R

A

E

A

I

-

R

-

Y

-

F

-

D

D

I

D

Q

E

G

V

E

V

Y

L

T

P

G

G

L

L

T

V

-

K

S

S

K

R

A

A

M

L

T

R

A

S

P

Q

D

C

G

G

K

T

I

L

R

R

I

L

P

P

L

L

N

N

E

-

E

I

S

S

K

E

Q

N

G

R

G

N

G

T

Q

A

I

I

E

A

E

H

F

A

L

L

M

S

Q

S

F

Y

N

R

G

G

E

S

G

G

I

V

Q

H

H
|
H

I

I

A

A

L

F

I

D

C

C

D

D

N

D

L

I

L

F

D

R

V

E

V

V

D

A

K

R

L

A

G

K

M

L

A

A

G

G

V

V

Q

P

L

L

A

L

T

E

A

I

P

P

N

L

E

N

V

-

Y

Y

Y

Y

E

D

M

D

L

L

D

A

T

A

R

R

L

F

P

D

G

F

H

D

G

D

Q

E

P

F

V

L

P

S

E

E

L

L

Q

A

S

Y

R

Y

G

N

I

V

L

L

L

Y

D

D

G

R

T

D

T

A

A

Q

D

G

G

G

T

E

P

-

R

-

L

-

L

L

L

F

Q

H

I

V

F

Y

S

T

T

E

P

P

M

F

L

E

G

E

P

R

V

F

F

F

F

F

E
|
E

F

I

I

I

Q

Q

R

R

E

K

G

A

D

G

Y

Y

R

A

D

-

G

G

F

Y

G

G

E

A

G

A

N

N

F

V

K

A

A

V

L

R

F

L

E

A

S

A

L

M

E

A

R

K

binding magnesium ion: H443 (= H184), H518 (= H263), E596 (= E345)

Sites not aligning to the query:

binding magnesium ion: 136, 167, 193, 241

1t47A Structure of fe2-hppd bound to ntbc (see paper)
37% identity, 73% coverage: 94:373/381 of query aligns to 79:360/362 of 1t47A

query
sites
1t47A

A

A

E

E

H

H

G

G

P

D

S

G

A

V

C

V

G

D

L

L

A

A

F

I

R

E

V

V

K

P

D

D

A

A

H

R

K

A

A

A

Y

H

N

A

R

Y

A

A

L

I

E

E

L

H

G

G

A

A

-

R

-

S

-

V

-

A

Q

E

P

P

I

Y

E

E

I

L

P

K

T

D

G

E

P

H

M

G

E

T

L

V

R

V

L

L

P

A

A

A

I

I

K

A

G

T

I

Y

G

G

G

K

A

T

P

R

L

H

Y

T

L

L

I

V

D

D

R

R

F

T

-

G

E

Y

D

D

G

G

K

P

S

-

I

-

Y

Y

D

L

I

P

D

G

F

Y

E

V

F

A

I

A

E

A

G

P

V

I

D

V

R

E

R

P

P

P

A

A

G

H

H

R

G

T

L

F

N

Q

L

A

I

I

D

D

H
|
H

L

C

T

V

H

G

N

N

V

V

Y

E

R

L

G

G

R

R

M

M

G

N

F

E

W

W

A

V

N

G

F

F

Y

Y

E

N

K

K

L

V

F

M

G

G

F

F

R

T

E

N

I

M

R

K

Y

E

F

F

-

V

-

G

-

D

D

D

I

I

Q

A

G

T

E

E

Y

Y

T

S

G

A

L

L

T

M

S
|
S

K

K

A

V

M

V

T

A

A

D

P

G

D

T

G

L

K

K

I

V

R

K

I

F

P
|
P

L

I

N

N

E

E

E

P

S

A

-

L

K

A

Q

K

G

K

G

K

G

S

Q

Q

I

I

E

D

E

E

F

Y

L

L

M

E

Q

F

F

Y

N

G

G

G

E

A

G

G

I

V

Q

Q

H
|
H

I

I

A

A

L

L

I

N

C

T

D

G

N

D

L

I

L

V

D

E

V

T

V

V

D

R

K

T

L

M

G

R

M

A

A

A

G

G

V

V

Q

Q

L

F

A

L

T

D

A

T

P

P

N

D

E

S

V

-

Y

Y

Y

Y

E

D

M

T

L

L

D

G

T

E

R

W

L

V

P

G

G

D

H

T

G

R

Q

V

P

P

V

V

P

D

E

T

L

L

Q

R

S

E

R

L

G

K

I

I

L

L

L

A

D

D

G

-

T

R

T

D

A

E

D

D

G

G

T

-

P

-

R

-

L

Y

L

L

L

L

Q

Q

I

I

F
|
F

S

T

T

K

P

P

M

V

L

Q

G

D

-

R

-

P

P

T

V

V

F

F

F

F

E
|
E

F

I

I

I

Q

E

R

R

E

H

G

G

D

S

Y

M

R

-

D

-

G

G

F
|
F

G
|
G

E

K

G

G

N
|
N

F
|
F

K

K

A

A

L
|
L

F

F

E

E

S

A

L

I

E

E

R

R

D

E

Q

Q

binding fe (ii) ion: H172 (= H184), H255 (= H263), E334 (= E345)
binding 2-{hydroxy[2-nitro-4-(trifluoromethyl)phenyl]methylene}cyclohexane-1,3-dione: H172 (= H184), S215 (= S224), P228 (= P237), H255 (= H263), F321 (= F334), E334 (= E345), F344 (= F357), G345 (= G358), N348 (= N361), F349 (= F362), L352 (= L365)

7yvvA Acmp1, r-4-hydroxymandelate synthase
29% identity, 78% coverage: 75:371/381 of query aligns to 47:332/335 of 7yvvA

query
sites
7yvvA

N

N

G

D

I

I

N

R

F

F

I

V

L

F

N

S

R

E

-

P

-

L

-

V

E

D

P

D

H

H

S

P

Q

G

A

T

A

A

Y

Y

F

V

G

D

A

-

E

R

H

H

G

G

P

D

S

G

A

V

C

S

G

D

L

I

A

A

F

L

R

R

V

V

K

T

D

D

A

A

H

K

K

A

A

A

Y

F

N

E

R

E

A

A

L

V

E

A

L

R

G

G

A

A

Q

R

P

P

I

V

E

A

I

G

P

P

T

S

G

R

P

A

M

G

E

H

L

V

R

V

L

T

P

A

A

S

I

I

K

M

G

G

I

F

G

G

G

-

A

-

P

-

L

-

Y

-

L

-

I

-

D

-

R

-

F

-

E

-

D

-

G

-

K

-

S

-

I

-

Y

-

D

D

I

T

D

L

F

H

E

T

F

F

I

V

E

E

G

Y

V

P

D

D

R

G

R

P

P

P

A

A

G

P

H

I

G

A

L

V

N

N

L

A

-

E

-

E

-

P

-

G

-

A

-

L

-

L

-

E

I

I

D

D

H
|
H

L

F

T

A

H

V

N

C

V

V

Y

E

R

H

G

G

R

H

M

L

G

D

F

A

W

T

A

V

N

D

F

F

Y

Y

E

R

K

D

L

V

F

L

G

G

F

F

R

E

E

L

I

I

R

F

Y

A

F

E

D

T

I

I

Q

A

G

V

E

G

Y

S

T

Q

G

A

L

M

T

T

S

T

K

K

A

V

M

V

T

Q

A

S

P

K

D

S

G

G

K

S

I

V

R

T

I

F

P

T

L

L

N

I

E

E

-

P

E

D

S

T

K

S

Q

K

G

D

G

P

G

G

Q

H

I

I

E

D

E

D

F

F

L

L

M

K

Q

D

F

H

N

G

G

G

E

A

G

G

I

V

Q

Q

H
|
H

I

I

A

A

L

F

I

T

C

S

D

N

N

G

L

I

L

I

D

E

V

A

V

V

D

D

K

V

L

L

G

R

M

D

A

R

G

G

V

V

Q

E

L

F

A

M

T

G

A

T

P

P

N

A

E

S

V

Y

Y

Y

Y

A

E

D

M

L

L

L

D

Q

T

R

R

V

L

V

P

P

G

E

H

Q

G

-

Q

Y

P

S

V

V

P

P

E

E

L

L

Q

R

S

T

R

Q

G

Q

I

V

L

L

L

V

D

D

G

-

T

E

T

D

A

H

D

D

G

G

T

Q

P

-

R

-

L

-

L

L

L

Y

Q

Q

I

I

F

F

S

A

T

R

P

S

M

V

-

H

-

P

L

R

G

N

P

T

V

I

F

F

F

L

E
|
E

F

L

I

I

Q

E

R

R

E

L

G

G

D

-

Y

-

R

A

D

R

G

G

F

F

G

G

E

S

G

G

N

N

F

I

K

T

A

A

L

L

F

Y

E

Q

S

A

L

A

E

E

R

R

binding fe (iii) ion: H149 (= H184), H229 (= H263), E308 (= E345)

O52791 4-hydroxymandelate synthase; HMS; HmaS; 4-hydroxyphenylpyruvate dioxygenase II; EC 1.13.11.46 from Amycolatopsis orientalis (Nocardia orientalis) (see paper)
29% identity, 83% coverage: 54:370/381 of query aligns to 25:343/357 of O52791

query
sites
O52791

E

D

K

K

L

Y

G

A

F

F

T

A

L

V

V

A

A

G

K

T

H

S

R

R

S

S

K

A

D

D

-

H

-

R

V

S

V

I

L

A

Y

L

R

R

Q

Q

N

G

G

Q

I

V

N

T

F

L

I

V

L

L

N

T

R

E

-

P

-

T

-

S

E

D

P

R

H

H

S

P

Q

A

A

A

A

A

Y

Y

F

L

G

-

A

Q

E

T

H

H

G

G

P

D

S

G

A

V

C

A

G

D

L

I

A

A

F

M

R

A

V

T

K

S

D

D

A

V

H

A

K

A

A

A

Y

Y

N

E

R

A

A

A

L

V

E

R

L

A

G

G

A

A

Q

E

P

A

I

V

E

R

I

A

P

P

T

G

G

Q

P

H

M

S

E

E

-

A

-

A

L

V

R

T

L

T

P

A

A

T

I

I

K

G

G

G

I

F

G

G

G

D

A

V

P

V

L

H

Y

T

L

L

I

I

D

Q

R

R

F

-

E

-

D

D

G

G

K

T

S

S

I

A

Y

-

D

E

I

L

D

P

F

P

E

G

F

F

I

T

E

G

G

S

V

M

D

D

R

V

R

T

P

N

A

H

G

G

H

K

G

G

-

D

-

V

-

D

L

L

N

L

L

G

I

I

D

D

H

H

L

F

T

A

H

I

N

C

V

L

Y

N

R

A

G

G

R

D

M

L

G

G

F

P

W

T

A

V

N

E

F

Y

Y

Y

E

E

K

R

L

A

F

L

G

G

F

F

R

R

E

Q

I

I

R

F

Y

D

F

E

D

H

I

I

Q

V

G

V

E

G

Y

A

T

Q

G

A

L

M

T

N

S
|
S

K

T

A

V

M

V

T

Q

A

S

P

A

D

S

G

G

K

A

I

V

R

T

I

L

P
x
T

L

L

N

I

E

E

E

P

S

D

K

R

Q

N

G

A

G

D

-

P

G

G

Q

Q

I

I

E

D

E

E

F

F

L

L

M

K

Q

D

F

H

N

Q

G

G

E

A

G

G

I

V

Q

Q

H
|
H

I

I

A

A

L

F

I

N

C

S

D

N

N

D

L

A

L

V

D

R

V

A

V

V

D

K

K

A

L

L

G

S

M

E

A

R

G

G

V

V

Q

E

L

F

A

L

T

K

A

T

P

P

N

G

E

-

V

A

Y

Y

Y

Y

E

D

M

L

L

L

D

G

T

E

R

R

L

I

P

T

G

L

H

Q

G

T

Q

H

P

S

V

L

P

D

E

D

L

L

Q

R

S

A

R

T

G

N

I

V

L

L

L

A

D

D

G

-

T

-

T

-

A

-

D

E

G

D

T

H

P

G

R

G

L

Q

L

L

L

F

Q
|
Q

I

I

F

F

-

T

-

A

S

S

T

T

P

H

M

P

L

R

G

H

P

T

V

I

F

F

F

F

E

E

F

V

I

I

Q

E

R

R

E

Q

G

G

D

-

Y

-

R

A

D

G

G

T

F

F

G

G

E

S

G

S

N

N

F

I

K

K

A

A

L

L

F

Y

E

E

S

A

L

V

E

E

S201 (= S224) binding
T214 (≠ P237) binding
H241 (= H263) binding
Q305 (= Q332) binding

2r5vA Hydroxymandelate synthase crystal structure (see paper)
29% identity, 83% coverage: 54:370/381 of query aligns to 24:341/346 of 2r5vA

query
sites
2r5vA

E

D

K

K

L

Y

G

A

F

F

T

A

L

V

V

A

A

G

K

T

H

S

R

R

S

S

K

A

D

D

-

H

-

R

V

S

V

I

L

A

Y

L

R

R

Q

Q

N

G

G

Q

I

V

N

T

F

L

I

V

L

L

N

T

R

E

-

P

-

T

-

S

E

D

P

R

H

H

S

P

Q

A

A

A

A

A

Y

Y

F

L

G

-

A

Q

E

T

H

H

G

G

P

D

S

G

A

V

C

A

G

D

L

I

A

A

F

M

R

A

V

T

K

S

D

D

A

V

H

A

K

A

A

A

Y

Y

N

E

R

A

A

A

L

V

E

R

L

A

G

G

A

A

Q

E

P

A

I

V

E

R

I

A

P

P

-

G

T

Q

G

H

P

S

M

A

E

A

L

V

R

T

L

T

P

A

A

T

I

I

K

G

G

G

I

F

G

G

G

D

A

V

P

V

L

H

Y

T

L

L

I

I

D

Q

R

R

F

-

E

-

D

D

G

G

K

T

S

S

I

A

Y

-

D

E

I

L

D

P

F

P

E

G

F

F

I

T

E

G

G

S

V

M

D

D

R

V

R

T

P

N

A

H

G

G

H

K

G

G

-

D

-

V

-

D

L

L

N

L

L

G

I

I

D

D

H
|
H

L

F

T

A

H

I

N

C

V

L

Y

N

R

A

G

G

R

D

M

L

G

G

F

P

W

T

A

V

N

E

F

Y

Y

Y

E

E

K

R

L

A

F

L

G

G

F

F

R

R

E

Q

I

I

R
x
F

Y

D

F

E

D

H

I

I

Q

V

G

V

E

G

Y

A

T

Q

G

A

L

M

T

N

S
|
S

K

T

A
x
V

M

V

T

Q

A

S

P

A

D

S

G

G

K

A

I

V

R

T

I

L

P
x
T

L

L

N

I

E

E

E

P

S

D

K

R

Q

N

G

A

G

D

-

P

G

G

Q

Q

I

I

E

D

E

E

F

F

L

L

M

K

Q

D

F

H

N

Q

G

G

E

A

G

G

I

V

Q

Q

H
|
H

I

I

A

A

L

F

I

N

C

S

D

N

N

D

L

A

L

V

D

R

V

A

V

V

D

K

K

A

L

L

G

S

M

E

A

R

G

G

V

V

Q

E

L

F

A

L

T

K

A

T

P

P

N

G

E

-

V

A

Y

Y

Y

Y

E

D

M

L

L

L

D

G

T

E

R

R

L

I

P

T

G

L

H

Q

G

T

Q

H

P

S

V

L

P

D

E

D

L

L

Q

R

S

A

R

T

G

N

I

V

L

L

L

A

D

D

G

-

T

-

T

-

A

-

D

E

G

D

T

H

P

G

R

G

L

Q

L

L

L

F

Q
|
Q

I

I

F

F

-

T

-

A

S

S

T

T

P

H

M

P

L

R

G

H

P

T

V

I

F

F

F

F

E
|
E

F

V

I

I

Q

E

R

R

E

Q

G

G

D

-

Y

-

R

A

D

G

G

T

F
|
F

G

G

E

S

G

S

N

N

F
x
I

K

K

A

A

L

L

F

Y

E

E

S

A

L

V

E

E

binding cobalt (ii) ion: H159 (= H184), H239 (= H263), E318 (= E345)
binding (2S)-hydroxy(4-hydroxyphenyl)ethanoic acid: H159 (= H184), F186 (≠ R211), S199 (= S224), V201 (≠ A226), T212 (≠ P237), H239 (= H263), Q303 (= Q332), E318 (= E345), F328 (= F357), I333 (≠ F362)

Q02110 4-hydroxyphenylpyruvate dioxygenase; 4-hydroxyphenylpyruvic acid oxidase; 4HPPD; HPD; HPPDase; EC 1.13.11.27 from Sus scrofa (Pig) (see paper)
28% identity, 83% coverage: 66:380/381 of query aligns to 54:382/393 of Q02110

query
sites
Q02110

S

S

K

R

D

E

V

V

V

V

-

S

-

H

L

V

Y

V

R

K

Q

Q

N

D

G

K

I

I

N

V

F

F

I

V

L

F

N

S

R

S

E

A

P

L

H

N

S

P

Q

W

A

N

A

K

Y

E

F

M

G

G

-

D

-

H

-

L

A

V

E

K

H

H

G

G

P

D

S

G

A

V

C

K

G

D

L

I

A

A

F

F

R

E

V

V

K

E

D

D

A

C

H

D

K

Y

A

I

Y

V

N

Q

R

K

A

A

L

R

E

E

L

R

G

G

A

A

Q

I

P

I

I

V

E

R

I

-

P

-

T

-

G

-

P

-

M

E

E

E

L

V

R

C

L

C

P

A

A

A

I

D

K

V

G

R

I

G

G

H

G

H

A

T

P

P

L

L

Y

D

L

R

I

A

D

R

R

Q

F

V

E

W

D

E

G

G

K

T

S

L

I

V

Y

E

D

K

I

M

D

T

F

F

E

-

F

-

I

-

E

-

G

C

V

L

D

D

R

S

R

R

P

P

A

Q

-

P

-

S

-

Q

-

T

-

L

-

L

-

H

-

R

-

L

-

L

-

L

-

S

-

K

-

L

-

P

G

K

H

C

G

G

L

L

N

E

L

I

I

I

D

D

H

H

L

I

T

V

H

G

N

N

V

Q

Y

P

R

D

G

Q

R

E

M

M

G

E

F

S

W

A

A

S

N

Q

F

W

Y

Y

E

M

K

R

L

N

F

L

G

Q

F

F

R

H

E

R

I

F

R

W

Y

S

F

V

D

D

-

D

-

T

-

Q

I

I

Q

H

G

T

E

E

Y

Y

T

S

G

A

L

L

T

R

S

S

K

V

A

V

M

M

T

A

A

N

P

Y

D

E

G

E

K

S

I

I

R

K

I

M

P

P

L

I

N

N

E

E

E

P

S

A

K

P

-

G

Q

K

G

K

G

K

G

S

Q

Q

I

I

E

Q

E

E

F

Y

L

V

M

D

Q

Y

F

N

N

G

G

G

E

A

G

G

I

V

Q

Q

H

H

I

I

A

A

L

L

I

K

C

T

D

E

N

D

L

I

L

I

D

T

V

A

V

I

D

R

K

S

L

L

G

R

M

E

A

R

G

G

V

V

Q

E

L

F

A

L

T

A

A

V

P

P

N

F

E

-

V

T

Y

Y

Y

Y

E

K

M

Q

L

L

D

Q

T

E

R

K

L

L

P

K

G

S

H

A

G

K

Q

I

P

R

V

V

P

K

E

E

-

S

-

I

-

D

-

V

L

L

Q

E

S

E

R

L

G

K

I

I

L

L

L

V

D

D

G

Y

T

D

T

E

A

K

D

G

G

-

T

-

P

-

R

-

L

Y

L

L

L

L

Q

Q

I

I

F

F

S

T

T

K

P

P

M

M

L

Q

G

D

-

R

-

P

P

T

V

V

F

F

F

L

E

E

F

V

I

I

Q

Q

R

R

E

N

G

N

D

-

Y

-

R

H

D

Q

G

G

F

F

G

G

E

A

G

G

N

N

F

F

K

N

A

S

L

L

F

F

E

K

S

A

L

F

E

E

R

E

D

E

Q

Q

I

E

R

L

R

R

G

G

V

N

L

L

N

T

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylthreonine

3zgjB S221m v223f y359a mutant of 4-hydroxymandelate synthase from streptomyces coelicolor (see paper)
27% identity, 85% coverage: 52:373/381 of query aligns to 23:342/343 of 3zgjB

query
sites
3zgjB

V

L

F

V

E

D

K

S

L

L

G

G

F

F

T

V

L

P

V

L

A

A

-

V

-

A

-

G

-

P

-

A

-

T

-

G

K

T

H

H

R

D

S

R

K

R

D

S

V

T

V

V

L

L

Y

-

R

R

Q

S

N

G

G

E

I

V

N

T

F

L

I

V

L

V

N

T

R

Q

E

A

-

L

-

A

P

P

H

D

S

T

Q

P

A

V

A

A

Y

R

F

Y

G

V

A

E

E

R

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binding cobalt (ii) ion: H161 (= H184), H241 (= H263), E316 (= E345)
binding (r)-mandelic acid: H161 (= H184), T214 (≠ P237), I216 (≠ N239), H241 (= H263), Q301 (= Q332), E316 (= E345)

P32755 4-hydroxyphenylpyruvate dioxygenase; 4-hydroxyphenylpyruvic acid oxidase; 4HPPD; HPD; HPPDase; F Alloantigen; F protein; EC 1.13.11.27 from Rattus norvegicus (Rat) (see 2 papers)
26% identity, 83% coverage: 66:380/381 of query aligns to 54:382/393 of P32755

query
sites
P32755

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H183 (= H184) binding
H266 (= H263) binding
E349 (= E345) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylthreonine

5ec3A Structural insight into the catalyitc mechanism of human 4- hydroxyphenylpyruvate dioxygenase
26% identity, 83% coverage: 66:380/381 of query aligns to 46:374/376 of 5ec3A

query
sites
5ec3A

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