SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ac3H11_215 FitnessBrowser__acidovorax_3H11:Ac3H11_215 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 10 hits to proteins with known functional sites (download)

5bokA Ferredoxin component of 3-nitrotoluene dioxygenase from diaphorobacter sp. Strain ds2
65% identity, 97% coverage: 4:104/104 of query aligns to 2:102/102 of 5bokA

query
sites
5bokA

N

N

W

W

I

I

D

D

A

A

A

A

A

A

H

R

G

D

D

E

V

V

P

P

E

E

D

G

D

D

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N

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Y

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E

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A

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G

E

E

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I

F

Y

A

A

T

T

D

D

N

N

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T

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C

T

T

H
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H

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G

H

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A
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A

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M

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E

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C

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L

H
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H

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Q

G

G

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R

F

F

D

D

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V

R

C

D

T

G

G

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K

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C

A

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V

L

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T

E

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L

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K

S

T

Y

Y

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P

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V

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K

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I

E

E

G

N

G

M

R

R

V

V

W

M

L

L

A

K

L

L

D

D

binding fe2/s2 (inorganic) cluster: C43 (= C45), H45 (= H47), G46 (= G48), A48 (= A50), C62 (= C64), L64 (= L66), H65 (= H67), P80 (= P82)

P0A185 Naphthalene 1,2-dioxygenase system, ferredoxin component from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
60% identity, 100% coverage: 1:104/104 of query aligns to 1:104/104 of P0A185

query
sites
P0A185

M
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M

T

T

T

V

N

K

W

W

I

I

D

E

A

A

A

V

A

A

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Y

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Y

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A

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T

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D

N

N

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C

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T

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H

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G

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H

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Q

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F

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D

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G

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K

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C

A

A

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Y

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E

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N

G

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W

M

L

I

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D

L

L

D

S

M1 (= M1) modified: Initiator methionine, Removed
C45 (= C45) binding
H47 (= H47) binding
C64 (= C64) binding
H67 (= H67) binding

2qpzA Naphthalene 1,2-dioxygenase rieske ferredoxin (see paper)
59% identity, 99% coverage: 2:104/104 of query aligns to 1:103/103 of 2qpzA

query
sites
2qpzA

T

T

T

V

N

K

W

W

I

I

D

E

A

A

A

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A

A

H

L

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A

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Y

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G

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Y

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A

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T

D

D

N

N

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C

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T

H
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H

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G

H

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A

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D

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G

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Y

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E

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E

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E

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C

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L

H
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H

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Q

G
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G

K

R

F

F

D

D

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V

R

C

D

T

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G

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K

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L

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C

A

A

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P

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E

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N

L

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T

Y

Y

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I

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E

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N

G

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L

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A

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L

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S

binding fe2/s2 (inorganic) cluster: C44 (= C45), H46 (= H47), G47 (= G48), C63 (= C64), L65 (= L66), H66 (= H67), G68 (= G69), P81 (= P82)

2i7fA Sphingomonas yanoikuyae b1 ferredoxin (see paper)
50% identity, 73% coverage: 29:104/104 of query aligns to 27:100/102 of 2i7fA

query
sites
2i7fA

I

L

A

A

L

V

Y

Y

G

N

V

V

G

D

G

G

E

E

V

V

F

F

A

V

T

T

D

D

N

N

I

L

C
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C

T

T

H
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H

G
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G

H

N

A

A

R

M

L

L

C

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D

D

G

G

F

Y

L

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E

D

G

G

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I

I

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E

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C

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P

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x
F

H
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H

Q

G

G

G

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S

F

F

D

D

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A

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T

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G

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C

A

A

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Y

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I

I

E

E

G

D

G

G

R

-

V

-

W

W

L

V

A

C

L

I

D

D

binding fe2/s2 (inorganic) cluster: C43 (= C45), H45 (= H47), G46 (= G48), C62 (= C64), F64 (≠ L66), H65 (= H67)

1fqtA Crystal structure of the rieske-type ferredoxin associated with biphenyl dioxygenase (see paper)
43% identity, 88% coverage: 13:104/104 of query aligns to 11:103/109 of 1fqtA

query
sites
1fqtA

D

D

V

V

P

P

E

E

D

G

D

E

V

A

I

L

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K

L

V

A

E

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A

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G

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A

A

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I

Y

F

G

N

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D

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G

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E

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F

A

A

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C

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T

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H

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G

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A

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L

C

S

D

D

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G

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G

F

Y

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L

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C

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H

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G

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F

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R

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G

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K

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E

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D

G

N

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L

L

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D

L

F

D

E

binding fe2/s2 (inorganic) cluster: C43 (= C45), H45 (= H47), G46 (= G48), C63 (= C64), H66 (= H67), G68 (= G69), P82 (≠ V83)

2e4pA Crystal structure of bpha3 (oxidized form) (see paper)
39% identity, 94% coverage: 4:101/104 of query aligns to 1:99/108 of 2e4pA

query
sites
2e4pA

N

T

W

F

I

T

D

K

A
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A

A

C

A

S

H

V

G

D

D
x
E

V

V

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P

E

P

D

G

D

E

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L

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A

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A

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A

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Y

F

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N

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V

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D

G

G

E

E

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F

F

F

A

A

T

T

D

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N

D

I

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C
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C

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T

H
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H

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G

H

E

A

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L

C

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E

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G

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G

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Y

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E

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C

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H

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G

K

K

F

F

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C

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V

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R

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T

G

G

K

K

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K

C

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C

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Y

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P

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I

K

R

I

I

E

E

G

G

G

R

R

D

V

V

W

L

L

V

binding fe2/s2 (inorganic) cluster: C42 (= C45), H44 (= H47), G45 (= G48), C62 (= C64), H65 (= H67), G67 (= G69)
binding alpha-D-glucopyranose: A5 (= A8), E10 (≠ D13)

3dqyA Crystal structure of toluene 2,3-dioxygenase ferredoxin (see paper)
38% identity, 97% coverage: 4:104/104 of query aligns to 1:101/106 of 3dqyA

query
sites
3dqyA

N

T

W

W

I

T

D

Y

A

I

A

L

A

R

H

Q

G

G

D

D

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L

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A

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C

G

N

V

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D

G

G

E

E

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F

F

F

A

A

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Q

N

D

I

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C

T

T

H
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H

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G

H

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A

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R

A

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L

C

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D

D

G

G

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Y

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L

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E

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C

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L

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H

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F

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G

K

K

F

F

D

C

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V

R

R

D

T

G

G

K

K

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K

C

A

A

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E

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G

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L

L

D

D

binding fe2/s2 (inorganic) cluster: C42 (= C45), H44 (= H47), G45 (= G48), C61 (= C64), H64 (= H67), G66 (= G69)

Q8GI16 Ferredoxin CarAc; Carbazole 1,9a-dioxygenase, ferredoxin component; CARDO from Pseudomonas resinovorans (see 2 papers)
37% identity, 98% coverage: 1:102/104 of query aligns to 1:103/107 of Q8GI16

query
sites
Q8GI16

M

M

T

N

T

Q

N

I

W

W

I

L

D

K

A

V

A

C

A

A

H

A

G

S

D

D

V

M

P

Q

E

P

D

G

D

T

V

I

I

R

G

R

L

V

A

N

-

R

V

V

A

G

G

A

R

A

D

P

I

L

A

A

L

V

Y

Y

G

R

V

V

G

G

G

D

E

Q

V

F

F

Y

A

A

T

T

D

E

N

D

I

T

C
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C

T

T

H
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H

G

G

H

I

A

A

R

S

L

L

C

S

D

E

G

G

F

T

L

L

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D

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G

H

D

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I

E

E

C
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C

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F

H
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H

Q

G

G

G

K

A

F

F

D

N

V

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C

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G

G

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M

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A

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E

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A

A

C46 (= C45) binding
H48 (= H47) binding
C65 (= C64) binding
H68 (= H67) binding

4nbbE Carbazole- and oxygen-bound oxygenase with ile262 replaced by val and ferredoxin complex of carbazole 1,9a-dioxygenase (see paper)
37% identity, 94% coverage: 5:102/104 of query aligns to 4:102/114 of 4nbbE

query
sites
4nbbE

W

W

I

L

D

K

A

V

A

C

A

A

H

A

G

S

D

D

V

M

P

Q

E

P

D

G

D

T

V

I

I

R

G

R

L

V

-

N

A

R

V

V

A

G

G

A

R

A

D

P

I

L

A

A

L

V

Y

Y

G

R

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V

G

G

G

D

E

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F

F

Y

A

A

T

T

D

E

N

D

I

T

C
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C

T

T

H
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H

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G

H

I

A

A

R

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L

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C

S

D

E

G

G

F

T

L

L

E

D

G

G

H

D

E

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I

I

E

E

C
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C

P

P

L
x
F

H
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H

Q

G

G
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G

K

A

F

F

D

N

V

V

R

C

D

T

G

G

K

M

P

P

T

A

C

S

A

S

P

P

V

C

T

T

E

V

P

P

L

L

R

G

S

V

Y

F

P

E

V

V

K

E

I

V

E

K

G

E

G

G

R

E

V

V

W

Y

L

V

A

A

binding fe2/s2 (inorganic) cluster: C45 (= C45), H47 (= H47), G48 (= G48), C64 (= C64), F66 (≠ L66), H67 (= H67), G69 (= G69)

3gceA Ferredoxin of carbazole 1,9a-dioxygenase from nocardioides aromaticivorans ic177 (see paper)
42% identity, 76% coverage: 24:102/104 of query aligns to 22:102/104 of 3gceA

query
sites
3gceA

V

V

A

D

G

G

R

D

D

E

I

V

A

M

L

V

Y

V

G

R

V

D

G

G

G

D

E

S

V

V

F

Y

A

A

T

I

D

S

N

N

I

L

C
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C

T

S

H
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H

G
x
A

H

E

A

A

R

Y

L

L

C

D

D

M

G

G

F

V

L

F

-

H

-

A

E

E

G

S

H

L

E

E

I

I

E

E

C
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C

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P

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L

H
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H

Q

V

G
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G

K

R

F

F

D

D

V

V

R

R

D

T

G

G

K

A

P

P

T

T

C

A

A

L

P
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P

V

C

T

V

E

L

P

P

L

V

R

R

S

A

Y

Y

P

D

V

V

K

V

I

V

E

D

G

G

G

T

R

E

V

I

W

L

L

V

A

A

binding fe2/s2 (inorganic) cluster: C43 (= C45), H45 (= H47), A46 (≠ G48), C64 (= C64), H67 (= H67), G69 (= G69), P82 (= P82)

Query Sequence

>Ac3H11_215 FitnessBrowser__acidovorax_3H11:Ac3H11_215
MTTNWIDAAAHGDVPEDDVIGLAVAGRDIALYGVGGEVFATDNICTHGHARLCDGFLEGH
EIECPLHQGKFDVRDGKPTCAPVTEPLRSYPVKIEGGRVWLALD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory