SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ac3H11_2251 FitnessBrowser__acidovorax_3H11:Ac3H11_2251 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0AC81 Lactoylglutathione lyase; Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase; EC 4.4.1.5 from Escherichia coli (strain K12) (see paper)
74% identity, 98% coverage: 1:129/131 of query aligns to 7:134/135 of P0AC81

query
sites
P0AC81

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E56 (= E51) binding
H74 (= H69) binding in other chain
E122 (= E117) binding in other chain

Sites not aligning to the query:

5 binding

1fa6A Crystal structure of the co(ii)-bound glyoxalase i of escherichia coli (see paper)
78% identity, 92% coverage: 1:120/131 of query aligns to 7:125/128 of 1fa6A

query
sites
1fa6A

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active site: E56 (= E51), H74 (= H69), E122 (= E117)
binding cobalt (ii) ion: E56 (= E51), H74 (= H69), E122 (= E117)

Sites not aligning to the query:

active site: 5
binding cobalt (ii) ion: 5

1fa5A Crystal structure of the zn(ii)-bound glyoxalase i of escherichia coli (see paper)
78% identity, 92% coverage: 1:120/131 of query aligns to 7:125/128 of 1fa5A

query
sites
1fa5A

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active site: E56 (= E51), H74 (= H69), E122 (= E117)
binding zinc ion: E56 (= E51), H74 (= H69), E122 (= E117)

Sites not aligning to the query:

active site: 5
binding zinc ion: 5

5d7zA Crystal structure of glyoxalase i from zea mays (see paper)
57% identity, 92% coverage: 3:123/131 of query aligns to 17:136/281 of 5d7zA

query
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5d7zA

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active site: E64 (= E51), H82 (= H69), E130 (= E117)

Sites not aligning to the query:

active site: 13
binding nickel (ii) ion: 276, 281

6bnnA Crystal structure of v278e-glyoxalase i mutant from zea mays in space group p4(1)2(1)2 (see paper)
57% identity, 92% coverage: 3:123/131 of query aligns to 23:142/282 of 6bnnA

query
sites
6bnnA

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active site: E70 (= E51), H88 (= H69), E136 (= E117)
binding cobalt (ii) ion: H88 (= H69), E136 (= E117)
binding glutathione: R112 (= R93), V117 (= V98), K118 (= K99)

Sites not aligning to the query:

Q948T6 Lactoylglutathione lyase; Aldoketomutase; Allergen Glb33; Glyoxalase I; Glx I; Glyoxylase I 11; OsGLYI-11; OsGLYI11; Ketone-aldehyde mutase; Methylglyoxalase; PP33; S-D-lactoylglutathione methylglyoxal lyase; Allergen Ory s Glyoxalase I; EC 4.4.1.5 from Oryza sativa subsp. japonica (Rice) (see paper)
56% identity, 91% coverage: 3:121/131 of query aligns to 31:149/291 of Q948T6

query
sites
Q948T6

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E78 (= E51) mutation to Q: No effect on the activity.
E145 (= E117) mutation to Q: Loss of activity.

Sites not aligning to the query:

209 E→D: Loss of activity.

4mttA Ni- and zn-bound gloa2 at low resolution (see paper)
48% identity, 92% coverage: 1:121/131 of query aligns to 7:126/128 of 4mttA

query
sites
4mttA

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active site: E56 (= E51), H74 (= H69), E122 (= E117)
binding zinc ion: E56 (= E51)

Sites not aligning to the query:

active site: 5
binding zinc ion: 5

2c21A Specificity of the trypanothione-dependednt leishmania major glyoxalase i: structure and biochemical comparison with the human enzyme (see paper)
46% identity, 92% coverage: 1:120/131 of query aligns to 8:121/139 of 2c21A

query
sites
2c21A

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active site: E57 (= E51), H75 (= H69), E118 (= E117)
binding nickel (ii) ion: E57 (= E51), H75 (= H69), E118 (= E117)

Sites not aligning to the query:

active site: 6
binding nickel (ii) ion: 6

7wszA Human glyoxalase i (with c-ter his tag) in glycerol-bound form (see paper)
38% identity, 92% coverage: 1:120/131 of query aligns to 28:168/183 of 7wszA

query
sites
7wszA

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binding glycerol: I50 (≠ L23), Q51 (≠ R24), K52 (≠ Q25), K80 (vs. gap), H119 (= H69), M150 (≠ T102), E165 (= E117)
binding zinc ion: E92 (= E51), H119 (= H69), E165 (= E117)

Sites not aligning to the query:

3w0uA Human glyoxalase i with an n-hydroxypyridone inhibitor
38% identity, 92% coverage: 1:120/131 of query aligns to 28:168/175 of 3w0uA

query
sites
3w0uA

M

M

L

L

R

R

V

V

G

K

N

D

L

P

Q

K

R

K

S

S

I

L

D

D

F

F

Y

Y

T

T

Q

R

V

V

L

L

G

G

M

M

Q

T

L

L

L

I

R

Q

Q

K

S

C

E

D

N

F

P

P

E

I

Y

M

K

K

Y
x
F

S

S

L
|
L

A

Y

F

F

L

L

G

A

F

Y

E

E

G

D

G

K

N

N

-

D

-

I

-

P

-

K

-

E

-

K

-

D

-

E

-

K

-

I

-

A

-

W

-

A

-

L

P

S

G

R

Q

K

A

A

E

T

I

L

E
|
E

L

L

T

T

Y

H

N

N

W

W

G

G

V

T

E

E

-

D

-

E

-

T

-

Q

S

S

Y

Y

E

H

I

N

G

G

T

N

A

S

-

D

-

P

-

R

-

G

Y

F

G

G

H
|
H

I

I

A

G

L

I

G

A

V

V

P

P

D

D

A

V

Y

Y

A

S

A

A

C

C

E

K

K

R

I

F

K

E

A

E

S

L

G

G

G

V

N

K

V

F

T

V

R
x
K

E

K

A

-

G

-

P

P

V

D

K

D

G

G

G

K

T
x
M

T

K

V

G

I

L

A

A

F

F

V

I

T

Q

D

D

P

P

D

D

G

G

Y

Y

K

W

I

I

E
|
E

L

I

I

L

Q

N

active site: E92 (= E51), H119 (= H69), E165 (= E117)
binding N-[3-(1-Hydroxy-6-oxo-4-phenyl-1,6-dihydro-pyridin-2-yl)-5-methanesulfonylamino-phenyl]-methanesulfonamide: F60 (≠ Y33), L62 (= L35), E92 (= E51), H119 (= H69), K143 (≠ R93), M150 (≠ T102), E165 (= E117)
binding zinc ion: E92 (= E51), H119 (= H69), E165 (= E117)

Sites not aligning to the query:

3w0tA Human glyoxalase i with an n-hydroxypyridone derivative inhibitor
38% identity, 92% coverage: 1:120/131 of query aligns to 28:168/176 of 3w0tA

query
sites
3w0tA

M

M

L

L

R

R

V

V

G

K

N

D

L

P

Q

K

R

K

S

S

I

L

D

D

F

F

Y

Y

T

T

Q

R

V

V

L

L

G

G

M

M

Q

T

L

L

L

I

R

Q

Q

K

S

C

E

D

N
x
F

P

P

E

I

Y

M

K

K

Y
x
F

S

S

L
|
L

A

Y

F
|
F

L

L

G

A

F

Y

E

E

G

D

G

K

N

N

-

D

-

I

-

P

-

K

-

E

-

K

-

D

-

E

-

K

-

I

-

A

-

W

-

A

-

L

P

S

G

R

Q

K

A

A

E

T

I

L

E
|
E

L

L

T

T

Y

H

N

N

W

W

G

G

V

T

E

E

-

D

-

E

-

T

-

Q

S

S

Y

Y

E

H

I

N

G

G

T

N

A

S

-

D

-

P

-

R

-

G

Y

F

G

G

H
|
H

I

I

A

G

L

I

G

A

V

V

P

P

D

D

A

V

Y

Y

A

S

A

A

C

C

E

K

K

R

I

F

K

E

A

E

S

L

G

G

G

V

N

K

V

F

T

V

R

K

E

K

A

-

G

-

P

P

V

D

K

D

G

G

G

K

T

M

T

K

V

G

I

L

A

A

F

F

V

I

T

Q

D

D

P

P

D

D

G

G

Y

Y

K

W

I

I

E
|
E

L

I

I

L

Q

N

active site: E92 (= E51), H119 (= H69), E165 (= E117)
binding N-[3-(1-hydroxy-6-oxo-4-phenyl-1,6-dihydropyridin-2-yl)phenyl]methanesulfonamide: F55 (≠ N28), F60 (≠ Y33), L62 (= L35), F64 (= F37), E92 (= E51), H119 (= H69), E165 (= E117)
binding zinc ion: E92 (= E51), H119 (= H69), E165 (= E117)

Sites not aligning to the query:

active site: 26
binding N-[3-(1-hydroxy-6-oxo-4-phenyl-1,6-dihydropyridin-2-yl)phenyl]methanesulfonamide: 26, 172, 176
binding zinc ion: 26

3vw9A Human glyoxalase i with an n-hydroxypyridone inhibitor (see paper)
38% identity, 92% coverage: 1:120/131 of query aligns to 28:168/176 of 3vw9A

query
sites
3vw9A

M

M

L

L

R

R

V

V

G

K

N

D

L

P

Q

K

R

K

S

S

I

L

D

D

F

F

Y

Y

T

T

Q

R

V

V

L

L

G

G

M

M

Q

T

L

L

L

I

R

Q

Q

K

S
x
C

E

D

N

F

P

P

E

I

Y

M

K

K

Y

F

S

S

L
|
L

A

Y

F

F

L

L

G

A

F

Y

E

E

G

D

G

K

N

N

-

D

-

I

-

P

-

K

-

E

-

K

-

D

-

E

-

K

-

I

-

A

-

W

-

A

-

L

P

S

G

R

Q

K

A

A

E

T

I

L

E
|
E

L

L

T

T

Y

H

N

N

W

W

G

G

V

T

E

E

-

D

-

E

-

T

-

Q

S

S

Y

Y

E

H

I

N

G

G

T

N

A

S

-

D

-

P

-

R

-

G

Y

F

G

G

H
|
H

I

I

A

G

L

I

G

A

V

V

P

P

D

D

A

V

Y

Y

A

S

A

A

C

C

E

K

K

R

I

F

K

E

A

E

S

L

G

G

G

V

N

K

V

F

T

V

R

K

E

K

A

-

G

-

P

P

V

D

K

D

G

G

G

K

T
x
M

T

K

V

G

I

L

A

A

F
|
F

V

I

T

Q

D

D

P

P

D

D

G

G

Y

Y

K

W

I

I

E
|
E

L

I

I

L

Q

N

active site: E92 (= E51), H119 (= H69), E165 (= E117)
binding 1-hydroxy-6-[1-(3-methoxypropyl)-1H-pyrrolo[2,3-b]pyridin-5-yl]-4-phenylpyridin-2(1H)-one: C53 (≠ S26), L62 (= L35), E92 (= E51), H119 (= H69), M150 (≠ T102), F155 (= F107), E165 (= E117)
binding zinc ion: E92 (= E51), H119 (= H69), E165 (= E117)

Sites not aligning to the query:

1qipA Human glyoxalase i complexed with s-p- nitrobenzyloxycarbonylglutathione (see paper)
38% identity, 92% coverage: 1:120/131 of query aligns to 28:168/176 of 1qipA

query
sites
1qipA

M

M

L

L

R
|
R

V

V

G

K

N

D

L

P

Q

K

R

K

S

S

I

L

D

D

F

F

Y

Y

T

T

Q

R

V

V

L

L

G

G

M

M

Q

T

L

L

L

I

R
x
Q

Q

K

S
x
C

E

D

N

F

P

P

E

I

Y

M

K

K

Y
x
F

S

S

L

L

A

Y

F
|
F

L

L

G

A

F

Y

E

E

G

D

G

K

N

N

-

D

-

I

-

P

-

K

-

E

-

K

-

D

-

E

-

K

-
x
I

-

A

-

W

-

A

-
x
L

P

S

G

R

Q

K

A

A

E

T

I

L

E
|
E

L

L

T
|
T

Y

H

N
|
N

W

W

G

G

V

T

E

E

-

D

-

E

-

T

-

Q

S

S

Y

Y

E

H

I

N

G

G

T

N

A

S

-

D

-

P

-
x
R

-

G

Y

F

G

G

H
|
H

I

I

A

G

L

I

G

A

V

V

P

P

D

D

A

V

Y

Y

A

S

A

A

C

C

E

K

K

R

I

F

K

E

A

E

S

L

G

G

G

V

N

K

V

F

T

V

R

K

E

K

A

-

G

-

P

P

V

D

K

D

G

G

G

K

T
x
M

T

K

V

G

I

L

A

A

F
|
F

V

I

T

Q

D

D

P

P

D

D

G

G

Y

Y

K

W

I

I

E
|
E

L

I

I

L

Q

N

active site: E92 (= E51), H119 (= H69), E165 (= E117)
binding s-p-nitrobenzyloxycarbonylglutathione: R30 (= R3), Q51 (≠ R24), C53 (≠ S26), F60 (≠ Y33), F64 (= F37), I81 (vs. gap), L85 (vs. gap), T94 (= T53), N96 (= N55), R115 (vs. gap), M150 (≠ T102), F155 (= F107)
binding zinc ion: E92 (= E51), H119 (= H69), E165 (= E117)

Sites not aligning to the query:

active site: 26
binding s-p-nitrobenzyloxycarbonylglutathione: 172
binding zinc ion: 26

1qinA Human glyoxalase i complexed with s-(n-hydroxy-n-p- iodophenylcarbamoyl) glutathione (see paper)
38% identity, 92% coverage: 1:120/131 of query aligns to 28:168/176 of 1qinA

query
sites
1qinA

M

M

L

L

R
|
R

V

V

G

K

N

D

L

P

Q

K

R

K

S

S

I

L

D

D

F

F

Y

Y

T

T

Q

R

V

V

L

L

G

G

M

M

Q

T

L

L

L

I

R

Q

Q

K

S

C

E

D

N

F

P

P

E

I

Y

M

K

K

Y
x
F

S

S

L
|
L

A

Y

F
|
F

L

L

G

A

F

Y

E

E

G

D

G

K

N

N

-

D

-

I

-

P

-

K

-

E

-

K

-

D

-

E

-

K

-

I

-

A

-

W

-

A

-

L

P

S

G

R

Q

K

A

A

E

T

I

L

E
|
E

L

L

T
|
T

Y

H

N
|
N

W

W

G

G

V

T

E

E

-

D

-

E

-

T

-

Q

S

S

Y

Y

E

H

I

N

G

G

T

N

A

S

-

D

-

P

-
x
R

-

G

Y

F

G

G

H
|
H

I

I

A

G

L

I

G

A

V

V

P

P

D

D

A

V

Y

Y

A

S

A

A

C

C

E

K

K

R

I

F

K

E

A

E

S

L

G

G

G

V

N

K

V

F

T

V

R
x
K

E

K

A

-

G

-

P

P

V

D

K

D

G
|
G

G
x
K

T
x
M

T

K

V

G

I

L

A

A

F

F

V

I

T

Q

D

D

P

P

D

D

G

G

Y

Y

K

W

I

I

E
|
E

L

I

I

L

Q

N

active site: E92 (= E51), H119 (= H69), E165 (= E117)
binding s-(n-hydroxy-n-iodophenylcarbamoyl)glutathione: R30 (= R3), F60 (≠ Y33), L62 (= L35), F64 (= F37), E92 (= E51), T94 (= T53), N96 (= N55), R115 (vs. gap), H119 (= H69), K143 (≠ R93), G148 (= G100), K149 (≠ G101), M150 (≠ T102), E165 (= E117)
binding zinc ion: E92 (= E51)

Sites not aligning to the query:

active site: 26
binding s-(n-hydroxy-n-iodophenylcarbamoyl)glutathione: 26, 172
binding zinc ion: 26

1froA Human glyoxalase i with benzyl-glutathione inhibitor (see paper)
38% identity, 92% coverage: 1:120/131 of query aligns to 28:168/176 of 1froA

query
sites
1froA

M

M

L

L

R
|
R

V

V

G

K

N

D

L

P

Q

K

R

K

S

S

I

L

D

D

F

F

Y

Y

T

T

Q

R

V

V

L

L

G

G

M

M

Q

T

L

L

L

I

R

Q

Q

K

S

C

E

D

N

F

P

P

E

I

Y

M

K

K

Y
x
F

S

S

L

L

A

Y

F

F

L

L

G

A

F

Y

E

E

G

D

G

K

N

N

-

D

-

I

-

P

-

K

-

E

-

K

-

D

-

E

-

K

-

I

-

A

-

W

-

A

-

L

P

S

G

R

Q

K

A

A

E

T

I

L

E
|
E

L

L

T
|
T

Y

H

N
|
N

W

W

G

G

V

T

E

E

-

D

-

E

-

T

-

Q

S

S

Y

Y

E

H

I

N

G

G

T

N

A

S

-

D

-

P

-
x
R

-

G

Y

F

G

G

H
|
H

I

I

A

G

L

I

G

A

V

V

P

P

D

D

A

V

Y

Y

A

S

A

A

C

C

E

K

K

R

I

F

K

E

A

E

S

L

G

G

G

V

N

K

V

F

T

V

R

K

E

K

A

-

G

-

P

P

V

D

K

D

G

G

G

K

T
x
M

T

K

V

G

I

L

A

A

F
|
F

V

I

T

Q

D

D

P

P

D

D

G

G

Y

Y

K

W

I

I

E
|
E

L

I

I

L

Q

N

active site: E92 (= E51), H119 (= H69), E165 (= E117)
binding s-benzyl-glutathione: R30 (= R3), F60 (≠ Y33), T94 (= T53), N96 (= N55), R115 (vs. gap), M150 (≠ T102), F155 (= F107), E165 (= E117)
binding zinc ion: E92 (= E51), H119 (= H69), E165 (= E117)

Sites not aligning to the query:

Q04760 Lactoylglutathione lyase; Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase; EC 4.4.1.5 from Homo sapiens (Human) (see 12 papers)
38% identity, 91% coverage: 1:119/131 of query aligns to 36:175/184 of Q04760

query
sites
Q04760

M

M

L

L

R

R

V

V

G

K

N

D

L

P

Q

K

R

K

S
|
S

I

L

D

D

F

F

Y

Y

T

T

Q

R

V

V

L

L

G

G

M

M

Q

T

L

L

L

I

R

Q

Q

K

S
x
C

E

D

N

F

P

P

E

I

Y

M

K

K

Y

F

S
|
S

L

L

A

Y

F

F

L

L

G

A

F

Y

E

E

G

D

G

K

N

N

-

D

-

I

-

P

-

K

-

E

-

K

-

D

-

E

-

K

-

I

-

A

-

W

-

A

-

L

P
x
S

G

R

Q

K

A

A

E
x
T

I

L

E
|
E

L

L

T
|
T

Y

H

N

N

W

W

G

G

V
x
T

E

E

-

D

-
x
E

-

T

-

Q

S

S

Y

Y

E

H

I

N

G

G

T

N

A

S

-

D

-

P

-

R

-

G

Y

F

G

G

H
|
H

I

I

A

G

L

I

G

A

V

V

P

P

D

D

A

V

Y

Y

A

S

A

A

C
|
C

E

K

K

R

I

F

K

E

A

E

S

L

G

G

G

V

N

K

V

F

T

V

R

K

E

K

A

-

G

-

P

P

V

D

K

D

G

G

G

K

T

M

T

K

V

G

I

L

A

A

F

F

V

I

T

Q

D

D

P

P

D

D

G

G

Y

Y

K

W

I

I

E
|
E

L

I

I

L

S45 (= S10) mutation to A: No effect on phosphorylation.
C61 (≠ S26) modified: Disulfide link with 139, Alternate; mutation to A: No effect on NO-mediated modification.
S69 (= S34) mutation to A: No effect on phosphorylation.
S94 (≠ P45) mutation to A: No effect on phosphorylation.
T98 (≠ E49) mutation to A: No effect on phosphorylation.
E100 (= E51) binding ; mutation to Q: Reduces enzyme activity by over 99%.
T102 (= T53) mutation to A: No effect on phosphorylation.
T107 (≠ V58) modified: Phosphothreonine; mutation to A: Loss of phosphorylation.
E111 (vs. gap) to A: in dbSNP:rs4746
H127 (= H69) binding in other chain
C139 (= C81) modified: S-glutathionyl cysteine; alternate; modified: Disulfide link with 61, Alternate; mutation to A: Impaired NO-mediated modification. Loss of NO-mediated modification; when associated with A-19 or A-20.
E173 (= E117) active site, Proton donor/acceptor; binding in other chain; mutation to Q: Abolishes enzyme activity.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
19 modified: Disulfide link with 20; C → Y: in dbSNP:rs17855424; C→A: No effect on NO-mediated modification. Impaired NO-mediated modification; when associated with A-20. Loss of NO-mediated modification; when associated with A-139.
20 modified: Disulfide link with 19; C→A: No effect on NO-mediated modification. Impaired NO-mediated modification; when associated with A-19. Loss of NO-mediated modification; when associated with A-139.
34 binding ; Q→E: Reduces enzyme activity by 99%.

7wt0A Human glyoxalase i (with c-ter his tag) in complex with tlsc702 (see paper)
38% identity, 91% coverage: 1:119/131 of query aligns to 35:174/185 of 7wt0A

query
sites
7wt0A

M

M

L

L

R

R

V

V

G

K

N

D

L

P

Q

K

R

K

S

S

I

L

D

D

F

F

Y

Y

T

T

Q

R

V

V

L

L

G

G

M

M

Q

T

L

L

L

I

R

Q

Q

K

S
x
C

E

D

N
x
F

P

P

E

I

Y

M

K

K

Y

F

S

S

L
|
L

A

Y

F
|
F

L

L

G

A

F

Y

E

E

G

D

G

K

N

N

-

D

-

I

-

P

-

K

-

E

-

K

-

D

-

E

-

K

-

I

-

A

-

W

-

A

-
x
L

P

S

G

R

Q

K

A

A

E

T

I

L

E
|
E

L

L

T

T

Y

H

N

N

W

W

G

G

V

T

E

E

-

D

-

E

-

T

-

Q

S

S

Y

Y

E

H

I

N

G

G

T

N

A

S

-

D

-

P

-

R

-

G

Y

F

G

G

H
|
H

I

I

A

G

L

I

G

A

V

V

P

P

D

D

A

V

Y

Y

A

S

A

A

C

C

E

K

K

R

I

F

K

E

A

E

S

L

G

G

G

V

N

K

V

F

T

V

R
x
K

E

K

A

-

G

-

P

P

V

D

K

D

G

G

G

K

T
x
M

T

K

V

G

I
x
L

A

A

F
|
F

V

I

T

Q

D

D

P

P

D

D

G

G

Y

Y

K

W

I

I

E
|
E

L

I

I

L

binding (~{E})-3-(1,3-benzothiazol-2-yl)-4-(4-methoxyphenyl)but-3-enoic acid: C60 (≠ S26), F62 (≠ N28), L69 (= L35), F71 (= F37), L92 (vs. gap), E99 (= E51), H126 (= H69), K150 (≠ R93), M157 (≠ T102), L160 (≠ I105), F162 (= F107), E172 (= E117)
binding zinc ion: E99 (= E51), H126 (= H69), E172 (= E117)

Sites not aligning to the query:

4pv5A Crystal structure of mouse glyoxalase i in complexed with 18-beta- glycyrrhetinic acid (see paper)
37% identity, 92% coverage: 1:120/131 of query aligns to 24:164/170 of 4pv5A

query
sites
4pv5A

M

M

L

L

R

R

V

I

G

K

N

D

L

P

Q

K

R

K

S

S

I

L

D

D

F

F

Y

Y

T

T

Q

R

V

V

L

L

G

G

M

L

Q

T

L

L

R

Q

Q

K

S

L

E

D

N

F

P

P

E

A

Y

M

K

K

Y

F

S

S

L

L

A

Y

F

F

L

L

G

A

F

Y

E

E

G

D

G

K

N

N

-

D

-

I

-

P

-

K

-

D

-

K

-

S

-

E

-

K

-

T

-

A

-

W

-

T

-

F

P

S

G

R

Q

K

A

A

E

T

I

L

E
|
E

L

L

T

T

Y

H

N

N

W

W

G

G

V

T

E

E

-

D

-

E

-

T

-

Q

S

S

Y

Y

E

H

I

N

G

G

T

N

A

S

-

D

-

P

-
x
R

-

G

Y

F

G

G

H
|
H

I

I

A

G

L

I

G

A

V

V

P

P

D

D

A

V

Y

Y

A

S

A

A

C

C

E

K

K

R

I

F

K

E

A

E

S

L

G

G

G

V

N

K

V

F

T

V

R
x
K

E

K

A

-

G

-

P

P

V

D

K

D

G

G

G

K

T

M

T

K

V

G

I

L

A

A

F

F

V

I

T

Q

D

D

P

P

D

D

G

G

Y

Y

K

W

I

I

E
|
E

L

I

I

L

Q

N

active site: E88 (= E51), H115 (= H69), E161 (= E117)
binding (3beta,5beta,14beta)-3-hydroxy-11-oxoolean-12-en-29-oic acid: R111 (vs. gap), K139 (≠ R93)
binding zinc ion: E88 (= E51)

Sites not aligning to the query:

active site: 22
binding zinc ion: 22

4x2aA Crystal structure of mouse glyoxalase i complexed with baicalein (see paper)
37% identity, 92% coverage: 1:120/131 of query aligns to 22:162/167 of 4x2aA

query
sites
4x2aA

M

M

L

L

R

R

V

I

G

K

N

D

L

P

Q

K

R

K

S

S

I

L

D

D

F

F

Y

Y

T

T

Q

R

V

V

L

L

G

G

M

L

Q

T

L

L

R

Q

Q

K

S

L

E

D

N
x
F

P

P

E

A

Y

M

K

K

Y

F

S

S

L
|
L

A

Y

F

F

L

L

G

A

F

Y

E

E

G

D

G

K

N

N

-

D

-

I

-

P

-

K

-

D

-

K

-

S

-

E

-

K

-

T

-

A

-

W

-

T

-
x
F

P

S

G

R

Q

K

A

A

E

T

I

L

E
|
E

L

L

T

T

Y

H

N

N

W

W

G

G

V

T

E

E

-

D

-

E

-

T

-

Q

S

S

Y

Y

E

H

I

N

G

G

T

N

A

S

-

D

-

P

-

R

-

G

Y

F

G

G

H
|
H

I

I

A

G

L

I

G

A

V

V

P

P

D

D

A

V

Y

Y

A

S

A

A

C

C

E

K

K

R

I

F

K

E

A

E

S

L

G

G

G

V

N

K

V

F

T

V

R

K

E

K

A

-

G

-

P

P

V

D

K

D

G

G

G

K

T
x
M

T

K

V

G

I

L

A

A

F

F

V

I

T

Q

D

D

P

P

D

D

G

G

Y

Y

K

W

I

I

E
|
E

L

I

I

L

Q

N

active site: E86 (= E51), H113 (= H69), E159 (= E117)
binding 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one: F49 (≠ N28), L56 (= L35), F79 (vs. gap), E86 (= E51), H113 (= H69), M144 (≠ T102), E159 (= E117)
binding zinc ion: E86 (= E51), H113 (= H69), E159 (= E117)

Sites not aligning to the query:

active site: 20
binding 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one: 20, 166, 167
binding zinc ion: 20

6l0uB Crystal structure of mouse glyoxalase i complexed with a small molecule inhibitor
37% identity, 92% coverage: 1:120/131 of query aligns to 29:169/177 of 6l0uB

query
sites
6l0uB

M

M

L

L

R

R

V

I

G

K

N

D

L

P

Q

K

R

K

S

S

I

L

D

D

F

F

Y

Y

T

T

Q

R

V

V

L

L

G

G

M

L

Q

T

L

L

R

Q

Q

K

S

L

E

D

N

F

P

P

E

A

Y

M

K

K

Y

F

S

S

L

L

A

Y

F

F

L

L

G

A

F

Y

E

E

G

D

G

K

N

N

-

D

-

I

-

P

-

K

-

D

-

K

-

S

-

E

-

K

-

T

-

A

-

W

-

T

-

F

P

S

G

R

Q

K

A

A

E

T

I

L

E
|
E

L

L

T

T

Y

H

N

N

W

W

G

G

V

T

E

E

-

D

-

E

-

T

-

Q

S

S

Y

Y

E

H

I

N

G

G

T

N

A

S

-

D

-

P

-
x
R

-

G

Y

F

G

G

H
|
H

I

I

A

G

L

I

G

A

V

V

P

P

D

D

A

V

Y

Y

A

S

A

A

C

C

E

K

K

R

I

F

K

E

A

E

S

L

G

G

G

V

N

K

V

F

T

V

R
x
K

E

K

A

-

G

-

P

P

V

D

K
x
D

G
|
G

G

K

T

M

T

K

V

G

I

L

A

A

F

F

V

I

T

Q

D

D

P

P

D

D

G

G

Y

Y

K
x
W

I

I

E
|
E

L

I

I

L

Q

N

active site: E93 (= E51), H120 (= H69), E166 (= E117)
binding N-[4-(trifluoromethyloxy)phenyl]-1,3,4,9-tetrahydropyrido[3,4-b]indole-2-carbothioamide: R116 (vs. gap), K144 (≠ R93), D148 (≠ K99), G149 (= G100), W164 (≠ K115)
binding zinc ion: E93 (= E51), H120 (= H69), E166 (= E117)

Sites not aligning to the query:

active site: 27
binding zinc ion: 27

Query Sequence

>Ac3H11_2251 FitnessBrowser__acidovorax_3H11:Ac3H11_2251
MLRVGNLQRSIDFYTQVLGMQLLRQSENPEYKYSLAFLGFEGGNPGQAEIELTYNWGVES
YEIGTAYGHIALGVPDAYAACEKIKASGGNVTREAGPVKGGTTVIAFVTDPDGYKIELIQ
RAEGAAGAGLR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory