SitesBLAST

A9 (≠ P6) binding
NPFC 39:42 (≠ NPFD 36:39) binding
C66 (= C63) binding
123:134 (vs. 118:129, 92% identical) binding
D128 (= D123) to N: in GA2B; decreased protein stability; dbSNP:rs104894678
T154 (≠ E149) to M: in dbSNP:rs1130426
R164 (= R159) to Q: in GA2B; reduced electron transfer activity; dbSNP:rs104894677
E165 (= E160) mutation E->A,Q: Drastically increases interprotein electron transfer rates.
183:205 (vs. 178:200, 87% identical) Recognition loop
L195 (= L190) mutation to A: Severely impaired in complex formation with ACADM.
K200 (= K195) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
KAK 200:202 (= KAK 195:197) mutation to RAR: Does not abolish methylation by ETFBKMT.
KAKK 200:203 (= KAKK 195:198) mutation to RAKR: Does not abolish electron transfer activity. Abolishes sensitivity to inhibition by lysine methyltransferase ETFBKMT.
K203 (= K198) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.

2a1uB Crystal structure of the human etf e165betaa mutant (see paper)
64% identity, 100% coverage: 1:250/251 of query aligns to 1:252/252 of 2a1uB

query
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2a1uB

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binding adenosine monophosphate: A6 (≠ P6), V7 (= V7), K8 (= K8), N36 (= N36), C39 (≠ D39), S62 (= S62), C63 (= C63), P98 (= P96), V101 (= V99), L119 (= L117), G120 (= G118), K121 (= K119), Q122 (= Q120), A123 (= A121), D126 (= D124), C128 (≠ A126), N129 (= N127), Q130 (= Q128), T131 (= T129)
binding flavin-adenine dinucleotide: P37 (= P37), F38 (= F38), L182 (= L180)

Q2TBV3 Electron transfer flavoprotein subunit beta; Beta-ETF from Bos taurus (Bovine) (see paper)
63% identity, 100% coverage: 1:250/251 of query aligns to 4:255/255 of Q2TBV3

query
sites
Q2TBV3

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K200 (= K195) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
K203 (= K198) modified: N6,N6,N6-trimethyllysine; by ETFBKMT

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

Q9DCW4 Electron transfer flavoprotein subunit beta; Beta-ETF from Mus musculus (Mouse) (see paper)
63% identity, 100% coverage: 1:250/251 of query aligns to 4:255/255 of Q9DCW4

query
sites
Q9DCW4

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K200 (= K195) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
K203 (= K198) modified: N6,N6,N6-trimethyllysine; by ETFBKMT

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

6fahB Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
35% identity, 98% coverage: 1:245/251 of query aligns to 1:257/262 of 6fahB

query
sites
6fahB

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binding flavin-adenine dinucleotide: C6 (≠ P6), A7 (≠ V7), K8 (= K8), D38 (= D39), T61 (≠ S62), M62 (≠ C63), A93 (≠ L94), D94 (≠ Q95), T95 (≠ P96), T98 (≠ V99), L102 (= L103), A117 (≠ L117), G118 (= G118), Q120 (= Q120), A121 (= A121), G124 (≠ D124), T126 (≠ A126), A127 (≠ N127), Q128 (= Q128), V129 (≠ T129), T223 (≠ R216), V225 (≠ K218)

P53570 Electron transfer flavoprotein subunit beta; Beta-ETF; Electron transfer flavoprotein small subunit; ETFSS from Methylophilus methylotrophus (Bacterium W3A1) (see paper)
32% identity, 84% coverage: 1:211/251 of query aligns to 1:215/264 of P53570

query
sites
P53570

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x
V

Q
|
Q

A
x
S

I

S

D

D

D

Q

D

A

A
x
Y

N
x
A

Q
x
S

T
|
T

G

G

Q

I

M

S

L

V

A

A

A

S

L

Y

A

L

D

N

L

W

P

P

Q

H

A

A

T

A

F

V

A

V

S

A

K

D

V

L

E

Q

L

Y

-

K

-

P

A

G

A

D

D

N

K

K

V

A

S

V

V

I

T

R

R

R

E

E

V

L

D

E

G

G

-

M

L

L

E

Q

T

E

L

V

S

E

L

I

T

N

L

C

P

P

A

A

V

V

I

L

T

T

T

I

D

Q

L

L

R

G

L

I

N

N

E

K

P

P

R

R

Y

Y

V

A

T

S

L

L

P

R

N

G

I

I

M

K

K

Q

A

A

K

A

K

T

K

K

Q

P

L

I

D

E

T

E

V

V

K

S

P

L

E

A

D

D

L

I

G

G

V

L

A6 (≠ P6) binding
NEWD 36:39 (≠ NPFD 36:39) binding
GVQS 119:122 (≠ GKQA 118:121) binding
YAST 127:130 (≠ ANQT 126:129) binding

1o96A Structure of electron transferring flavoprotein for methylophilus methylotrophus. (see paper)
32% identity, 84% coverage: 1:211/251 of query aligns to 1:214/261 of 1o96A

query
sites
1o96A

M

M

R

K

I

I

L

L

V

V

P
x
A

V
|
V

K
|
K

R

Q

V

T

V

A

D

A

Y

L

N

E

V

E

K

D

V

F

R

E

V

I

K

R

S

-

D

D

N

G

T

M

G

D

V

V

D

D

I

E

A

D

N

F

V

M

K

M

M

Y

S

D

M

L

N
|
N

P

E

F
x
W

D
|
D

E

D

I

F

A

S

V

L

E

E

A

A

V

M

R

K

L

I

K

K

E

E

K

S

G

S

L

D

V

T

-

D

T

V

E

E

V

V

I

V

A

V

V

V

S
|
S

C
x
V

G

G

V

P

A

D

Q

R

C

V

Q

D

E

E

T

S

L

L

R

R

T

K

A

C

M

L

A

A

I

K

G

G

A

A

D

D

R

R

A

A

I

V

L

R

V

V

E

W

T

D

P

D

A

A

D

A

L

E

D

G

L

S

Q

D

P

A

L

I

A

V

V
|
V

A

G

K

R

L

I

L
|
L

K

T

A

E

L

V

V

I

D

K

K

K

E

E

Q

A

P

P

S

D

L

M

V

V

I

F

L
x
A

G
|
G

K
x
V

Q
|
Q

A
x
S

I

S

D

D

D

Q

D
x
A

A
x
Y

N
x
A

Q
x
S

T
|
T

G

G

Q

I

M

S

L

V

A

A

A

S

L

Y

A

L

D

N

L

W

P

P

Q

H

A

A

T

A

F

V

A

V

S

A

K

D

V

L

E

Q

L

Y

-

K

-

P

A

G

A

D

D

N

K

K

V

A

S

V

V

I

T

R

R

R

E

E

V

L

D

E

G

G

-

M

L

L

E

Q

T

E

L

V

S

E

L

I

T

N

L

C

P

P

A

A

V

V

I

L

T

T

T

I

D
x
Q

L

L

R

G

L

I

N

N

E

K

P

P

R

R

Y

Y

V

A

T

S

L

L

P

R

N

G

I

I

M

K

K

Q

A

A

K

A

K

T

K

K

Q

P

L

I

D

E

T

E

V

V

K

S

P

L

E

A

D

D

L

I

G

G

V

L

binding adenosine monophosphate: A6 (≠ P6), V7 (= V7), K8 (= K8), N35 (= N36), D38 (= D39), S62 (= S62), V63 (≠ C63), V99 (= V99), L103 (= L103), A117 (≠ L117), G118 (= G118), V119 (≠ K119), Q120 (= Q120), S121 (≠ A121), A125 (≠ D125), Y126 (≠ A126), A127 (≠ N127), S128 (≠ Q128), T129 (= T129)
binding flavin-adenine dinucleotide: W37 (≠ F38), Q120 (= Q120), Q182 (≠ D179)

5ol2B The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
31% identity, 98% coverage: 1:245/251 of query aligns to 1:255/260 of 5ol2B

query
sites
5ol2B

M

M

R

N

I

I

L

V

V

V

P
x
C

V
x
I

K
|
K

R

Q

V

V

V

P

D

D

Y

-

N

-

V

-

K

T

V

T

R

E

V

V

K

K

S

L

D

D

-

P

N

N

T

T

G

G

V

T

D

L

I

I

A

R

N

D

-

G

V

V

K

P

M

S

S

I

M

I

N

N

P

P

F

D

D
|
D

E

K

I

A

A

G

V

L

E

E

A

A

V

I

R

K

L

L

K

K

E

E

K

E

G

-

L

M

V

G

T

A

E

H

V

V

I

T

A

V

V

I

S
x
T

C
x
M

G

G

V

P

A

P

Q

Q

C

A

Q

D

E

M

T

A

L

L

R

K

T

E

A

A

M

L

A

A

I

M

G

G

A

A

D

D

R

R

A

G

I

I

L

L

V

L

E

T

T

D

P

R

A

A

D

F

L

A

D

G

L
x
A

Q
x
D

P
x
T

L

W

A

A

V
x
T

A

S

K

S

L

A

L
|
L

K

A

A

G

L

A

V

L

D

K

K

N

E

I

Q

D

P

F

S

D

L

I

V

I

I

I

L
x
A

G
|
G

K
x
R

Q
|
Q

A
|
A

I

I

D

D

D
x
G

D
|
D

A
x
T

N
x
A

Q
|
Q

T
x
V

G
|
G

Q

P

M

Q

L

I

A

A

A

E

L

H

A

L

D

N

L

L

P

P

Q

S

A

I

T

T

F

Y

A

A

S

E

K

E

V

I

E

K

L

T

A

E

A

G

D

E

K

Y

V

V

S

L

V

V

T

K

R

R

E

Q

V

F

D

E

G

D

G

C

L

C

E

H

T

D

L

L

S

K

L

V

T

K

L

M

P

P

A

C

V

L

I

I

T

T

D

L

L

K

R

D

L

M

N

N

E

T

P

P

R

R

Y

Y

V

M

T

K

L

V

P

G

N

R

I

I

M

Y

K

D

A

A

K

F

K

E

K

N

-

D

-

V

Q

E

L

T

D

W

T

T

V

V

K

K

-

D

-

I

-

E

-

V

-

D

P

P

E

S

D

N

L

L

G

G

V

L

D

K

V

G

A

S

P

P

R
x
T

-

S

-

V

-

F

-

K

-

S

-

F

L

T

K

K

T

S

L

V

K

K

-

P

-

A

-

G

-

T

-

I

V

Y

S

N

E

E

P

D

A

A

K

K

R

T

G

S

A

A

G

G

V

I

K

-

V

-

A

-

D

-

V

-

A

-

L

I

V

I

E

D

K

K

L

L

K

K

binding flavin-adenine dinucleotide: C6 (≠ P6), I7 (≠ V7), K8 (= K8), D38 (= D39), T60 (≠ S62), M61 (≠ C63), A92 (≠ L94), D93 (≠ Q95), T94 (≠ P96), T97 (≠ V99), L101 (= L103), A115 (≠ L117), G116 (= G118), R117 (≠ K119), Q118 (= Q120), A119 (= A121), G122 (≠ D124), D123 (= D125), T124 (≠ A126), A125 (≠ N127), Q126 (= Q128), V127 (≠ T129), G128 (= G130), T222 (≠ R216)

7koeA Electron bifurcating flavoprotein fix/etfabcx (see paper)
32% identity, 84% coverage: 3:212/251 of query aligns to 4:219/282 of 7koeA

query
sites
7koeA

I

V

L

V

V

V

P
x
C

V
x
I

K
|
K

R

Q

V

V

V

P

D

D

Y

T

-

T

N

N

V

V

K

R

V

I

R

D

V

R

K

K

S

T

D

N

N

N

T

L

G

V

V

R

D

E

I

-

A

-

N

G

V

V

K

P

M

S

S

I

M

I

N
|
N

P

P

F

D

D
|
D

E

E

I

R

A

A

V

L

E

E

E

L

A

A

V

S

R

Q

L

L

K

K

E

E

K

K

G

F

L

G

V

A

T

T

E

-

V

V

I

Y

A

V

V

I

S
x
T

C
x
M

G

G

V

P

A

P

Q

Q

C

A

Q

K

E

E

T

A

L

L

R

K

T

D

A

A

M

I

A

A

I

F

G

G

A

L

D

D

R

E

A

A

I

V

L

H

V

L

E

S

T

D

P

R

A

T

D

F

L

A

D

G

L
x
A

Q
x
D

P
x
T

L

L

A

A

V
x
T

A

T

K

Y

L

T

L
|
L

K

Y

A

W

L

G

V

I

D

K

K

K

E

I

Q

E

P

E

-

R

-

I

-

G

-

K

-

I

S

D

L

L

V

I

I

L

L
x
T

G
|
G

K

K

Q
|
Q

A
|
A

I

V

D
|
D

D
x
G

D

D

A
x
T

N
x
G

Q
|
Q

T
x
V

G

G

Q

P

M

G

L

L

A

A

A

T

L

R

A

F

D

G

L

Y

P

A

Q

L

A

G

T

A

F

Y

A

V

S

V

K

R

V

I

E

E

-

E

-

I

-

D

L

P

A

E

A

K

D

K

K

E

V

M

S

V

V

I

T

V

R

R

E

R

V

L

D

D

G

Q

G

G

L

F

E

E

T

K

L

I

S

R

L

L

T

K

L

L

P

P

A

A

V

V

I

L

T

T

T

I

D

T

L

D

R

E

L

L

N

N

E

K

P

P

R

R

Y

Y

V

A

T

D

L

L

P

P

N

N

I

L

M

I

K

R

A

A

K

I

K

R

K

Y

Q

E

L

P

D

I

T

V

V

W

K

T

P

H

E

K

D

D

L

L

G

G

V

L

D

D

binding flavin-adenine dinucleotide: C7 (≠ P6), I8 (≠ V7), K9 (= K8), N36 (= N36), D39 (= D39), T61 (≠ S62), M62 (≠ C63), A93 (≠ L94), D94 (≠ Q95), T95 (≠ P96), T98 (≠ V99), L102 (= L103), T121 (≠ L117), G122 (= G118), Q124 (= Q120), A125 (= A121), D127 (= D123), G128 (≠ D124), T130 (≠ A126), G131 (≠ N127), Q132 (= Q128), V133 (≠ T129)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 232

4l2iB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
33% identity, 80% coverage: 1:201/251 of query aligns to 1:199/263 of 4l2iB

query
sites
4l2iB

M

M

R

N

I

I

L

V

V

V

P
x
C

V
|
V

K
|
K

R

Q

V

V

V

P

D

D

Y

T

-

A

N

E

V

M

K

K

V

I

R

D

V

P

K

V

S

T

D

N

N

N

T

L

G

V

V

R

D

D

-

G

I

V

A

T

N

N

V

I

K

-

M

-

S

-

M

M

N

N

P
|
P

F
x
Y

D
|
D

E

Q

I
x
Y

A

A

V

L

E

E

E

T

A

A

V

L

R

Q

L

L

K

K

E

D

K

E

G

-

L

L

V

G

T

A

E

H

V

V

I

T

A

V

V

I

S
x
T

C
x
M

G
|
G

V
x
P

A

P

Q

H

C

A

Q

E

E

S

T

V

L

L

R

R

T

D

A

C

M

L

A

A

I

V

G

G

A

A

D

D

R

E

A

A

I

K

L

L

V
|
V

E
x
S

T
x
D

P
x
R

A

A

D
x
F

L
x
G

D
x
G

L
x
A

Q
x
D

P
x
T

L

L

A

A

V
x
T

-

S

A

A

K

A

L
x
M

L

A

K

N

A

T

L

I

V

K

D

H

K

F

E

G

Q

V

P

P

S

D

L

L

V

I

I

L

L
x
C

G
|
G

K

R

Q
|
Q

A
|
A

I

I

D

D

D
x
G

D
|
D

A
x
T

N
x
A

Q
|
Q

T
x
V

G

G

Q

P

M

E

L

I

A

A

A

E

L

H

A

L

D

G

L

L

P

P

Q

Q

A

V

T

T

F

A

A

A

S

L

K

K

V

V

E

Q

L

V

A

K

A

D

D

D

K

T

V

V

S

V

V

V

T

D

R

R

E

D

V

N

D

E

G

Q

G

M

L

S

E

M

T

T

L

F

S

T

L

M

T

K

L

M

P

P

A

C

V

V

I

V

T

T

T

V

D

-

L

M

R

R

L

S

N

K

E

D

P

L

R

R

Y

F

V

A

T

S

L

I

P

R

N

G

I

K

M

M

K

K

A

A

K

R

K

K

K

A

Q

E

L

I

binding flavin-adenine dinucleotide: C6 (≠ P6), V7 (= V7), K8 (= K8), P36 (= P37), Y37 (≠ F38), D38 (= D39), Y40 (≠ I41), T60 (≠ S62), M61 (≠ C63), A92 (≠ L94), D93 (≠ Q95), T94 (≠ P96), T97 (≠ V99), M101 (≠ L102), C116 (≠ L117), G117 (= G118), Q119 (= Q120), A120 (= A121), G123 (≠ D124), D124 (= D125), T125 (≠ A126), A126 (≠ N127), Q127 (= Q128), V128 (≠ T129)
binding nicotinamide-adenine-dinucleotide: M61 (≠ C63), G62 (= G64), P63 (≠ V65), V84 (= V86), S85 (≠ E87), D86 (≠ T88), R87 (≠ P89), F89 (≠ D91), G90 (≠ L92), G91 (≠ D93), A92 (≠ L94)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 221, 223

4kpuB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
33% identity, 80% coverage: 1:201/251 of query aligns to 1:199/263 of 4kpuB

query
sites
4kpuB

M

M

R

N

I

I

L

V

V

V

P
x
C

V
|
V

K
|
K

R

Q

V

V

V

P

D

D

Y

T

-

A

N

E

V

M

K

K

V

I

R

D

V

P

K

V

S

T

D

N

N

N

T

L

G

V

V

R

D

D

-

G

I

V

A

T

N

N

V

I

K

-

M

-

S

-

M

M

N

N

P

P

F
x
Y

D
|
D

E

Q

I
x
Y

A

A

V

L

E

E

E

T

A

A

V

L

R

Q

L

L

K

K

E

D

K

E

G

-

L

L

V

G

T

A

E

H

V

V

I

T

A

V

V

I

S
x
T

C
x
M

G

G

V

P

A

P

Q

H

C

A

Q

E

E

S

T

V

L

L

R

R

T

D

A

C

M

L

A

A

I

V

G

G

A

A

D

D

R

E

A

A

I

K

L

L

V

V

E

S

T

D

P

R

A

A

D

F

L

G

D

G

L
x
A

Q
x
D

P
x
T

L

L

A

A

V
x
T

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A

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M

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N

D

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binding flavin-adenine dinucleotide: C6 (≠ P6), V7 (= V7), K8 (= K8), Y37 (≠ F38), D38 (= D39), Y40 (≠ I41), T60 (≠ S62), M61 (≠ C63), A92 (≠ L94), D93 (≠ Q95), T94 (≠ P96), T97 (≠ V99), M101 (≠ L102), C116 (≠ L117), G117 (= G118), Q119 (= Q120), A120 (= A121), G123 (≠ D124), D124 (= D125), T125 (≠ A126), A126 (≠ N127), Q127 (= Q128), V128 (≠ T129)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 221, 223

7qh2B Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
33% identity, 82% coverage: 2:207/251 of query aligns to 3:202/265 of 7qh2B

query
sites
7qh2B

R

K

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x
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K

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E

binding flavin-adenine dinucleotide: C7 (≠ P6), I8 (≠ V7), K9 (= K8), D39 (= D39), S61 (= S62), M62 (≠ C63), A93 (= A90), D94 (= D91), V95 (≠ L92), T98 (≠ Q95), L102 (≠ V99), C117 (≠ L117), G118 (= G118), K119 (= K119), Q120 (= Q120), T121 (≠ A121), G124 (≠ D124), D125 (= D125), T126 (≠ A126), A127 (≠ N127), Q128 (= Q128), V129 (≠ T129)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 226

5ow0B Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
34% identity, 68% coverage: 38:207/251 of query aligns to 38:204/251 of 5ow0B

query
sites
5ow0B

F
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binding adenosine monophosphate: D39 (= D39), S61 (= S62), Y62 (≠ C63), I95 (≠ V99), L99 (= L103), A114 (≠ L117), G115 (= G118), Q117 (= Q120), S118 (≠ A121), R123 (≠ A126), G124 (≠ N127), V125 (≠ Q128), V126 (≠ T129), P127 (≠ G130)
binding flavin-adenine dinucleotide: Y38 (≠ F38), Q117 (= Q120), S119 (≠ I122)

Sites not aligning to the query:

Query Sequence

>Ac3H11_2706 FitnessBrowser__acidovorax_3H11:Ac3H11_2706
MRILVPVKRVVDYNVKVRVKSDNTGVDIANVKMSMNPFDEIAVEEAVRLKEKGLVTEVIA
VSCGVAQCQETLRTAMAIGADRAILVETPADLDLQPLAVAKLLKALVDKEQPSLVILGKQ
AIDDDANQTGQMLAALADLPQATFASKVELAADKVSVTREVDGGLETLSLTLPAVITTDL
RLNEPRYVTLPNIMKAKKKQLDTVKPEDLGVDVAPRLKTLKVSEPAKRGAGVKVADVAAL
VEKLKNEAKVI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory