SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ac3H11_2717 FitnessBrowser__acidovorax_3H11:Ac3H11_2717 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P96855 Acyl-CoA dehydrogenase FadE34; ACAD; 3-oxochol-4-en-24-oyl-CoA dehydrogenase; EC 1.3.99.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
33% identity, 99% coverage: 4:328/329 of query aligns to 399:709/711 of P96855

query
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P96855

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E581 (= E196) mutation to Q: Displays less than 1% activity with cholyl-CoA as substrate. Still binds FAD.

Sites not aligning to the query:

236 R→A: Displays less than 2% activity with cholyl-CoA as substrate. Cannot bind FAD.

P71858 Acyl-CoA dehydrogenase FadE29; ACAD; 3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA dehydrogenase beta subunit; 3-oxo-4-pregnene-20-carboxyl-CoA dehydrogenase beta subunit; EC 1.3.99.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
32% identity, 97% coverage: 4:323/329 of query aligns to 59:376/387 of P71858

query
sites
P71858

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E241 (= E196) mutation to Q: Unable to dehydrogenate pregnene-carboxyl-CoA ester.

8hk0B Crystal structure of fic32-33 complex from streptomyces ficellus nrrl 8067 (see paper)
32% identity, 97% coverage: 4:323/329 of query aligns to 60:370/379 of 8hk0B

query
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8hk0B

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binding flavin-adenine dinucleotide: V126 (≠ I70), Y128 (≠ M72), T129 (≠ S73), G134 (= G78), S135 (= S79), F159 (≠ W107), S160 (≠ T108), L161 (≠ T109), G354 (≠ S307), S358 (≠ R311), T361 (= T314), E363 (= E316)

6wy9A Tcur3481-tcur3483 steroid acad g363a variant (see paper)
30% identity, 99% coverage: 4:328/329 of query aligns to 56:377/380 of 6wy9A

query
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6wy9A

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E

R

L

T

A

L

Q

T

Q

G

V

I

L

L

D

G

V

A

A

A

P

G

A

R

V

I

G

R

G

P

G

E

S

S

D

P

H

G

A

A

Q

V

V

L

L

A

A

G

A

Q

L

I

M

E

Q

Q

M

L

A

S

P

R

S

Q

-

G

-

I

-

V

F

N

S

T

L
x
F

R

A

G

G

T
x
V

R

N

E
|
E

I

V

L

L

R

R

G

D

I

M

I

V

A

A

R
x
T

-

L

G

G

L

L

G

G

L

M

active site: Y122 (≠ M72), T123 (≠ S73), E237 (= E196), T372 (≠ R324)
binding dihydroflavine-adenine dinucleotide: I120 (= I70), Y122 (≠ M72), T123 (≠ S73), G128 (= G78), T129 (≠ S79), F153 (≠ W107), S155 (≠ T109), F358 (≠ L310), V362 (≠ T314), E364 (= E316)

6wy8B Tcur3481-tcur3483 steroid acad (see paper)
30% identity, 99% coverage: 4:328/329 of query aligns to 60:381/384 of 6wy8B

query
sites
6wy8B

P

P

H

K

Q

E

Y

Y

G

G

H

Q

D

G

A

R

S

S

P

I

W

S

A

E

R

Q

Y

Y

V

V

V

L

I

F

E

D

E

E

L

V

L

Q

A

R

A

A

G

G

A

L

P

P

V

F

S

P

A

-

H

-

W

F

I

V

A

T

D

V

R

N

Q

T

S

V

G

G

P

P

L

T

I

L

L

M

R

K

Y

Y

G

G

T

T

E

E

A

E

Q

Q

R

K

Q

K

K

K

Y

Y

L

L

P

P

G

G

I

I

C

L

K

S

G

G

E

D

V

I

Y

V

F

F

C

A

I
|
I

G

G

M
x
Y

S
x
T

E

E

P

P

N

G

S

A

G
|
G

S
x
T

D

D

L

L

A

A

S

S

I

L

R

T

S

T

R

R

A

A

V

V

R

R

D

D

E

-

A

-

G

G

D

D

G

-

S

E

W

F

R

V

L

I

S

D

G

G

Q

S

K

K

V

I

W
x
F

T

T

T
x
S

N

G

A

A

H

N

H

T

A

A

Q

D

Y

Y

M

I

I

W

A

L

L

A

V

C

R

R

T

T

G

D

A

P

A

-

T

-

G

-

E

-

K

E

A

A

A

P

R

K

H

H

E

K

G

G

M

I

S

S

Q

I

F

I

I

I

I

V

D

P

L

T

K

D

A

A

A

E

G

G

V

F

T

S

V

W

R

S

P

P

I

I

R

Q

D

T

L

V

A

-

G

G

E

M

H

V

F

V

N

T

E

A

V

T

Y

Y

L

Y

D

S

D

G

V

V

R

R

L

V

D

P

A

V

D

S

A

E

L

V

I

V

G

G

T

E

E

I

G

N

Q

G

G

G

W

W

E

K

Q

L

V

I

T

T

A

T

E

Q

L

L

A

N

F

H

E
|
E

R

R

S

I

G

G

P

L

E

A

R

A

F

L

L

G

S

G

S

R

M

M

A

I

L

R

L

L

H

W

T

E

L

D

I

V

D

V

A

A

V

W

G

A

A

R

Q

D

P

N

D

G

A

V

L

L

Q

E

A

Q

H

P

A

W

V

V

-

R

-

D

-

L

G

A

R

R

L

T

S

Y

A

A

R

K

L

L

V

E

V

A

L

M

R

R

-

L

-

N

-

W

Q

K

M

M

S

T

L

I

A

A

V

V

T

E

A

N

E

D

L

E

A

L

A

T

G

G

G

A

N

D

P

-

A

-

W

-

A

-

A

A

S

G

C

A

L

T

K

K

D

A

L

Y

G

G

A

T

A

E

F

T

E

H

Q

I

E

D

I

V

P

Q

E

R

L

T

A

L

Q

T

Q

G

V

I

L

L

D

G

V

A

A

A

P

G

A

R

V

I

G

R

G

P

G

E

S

S

D

P

H

G

A

A

Q

V

V

L

L

A

A

G

A

Q

L

I

M

E

Q

Q

M

L

A

S

P

R

S

Q

-

G

-

I

-
x
V

F

N

S

T

L
x
F

R
x
G

G

G

T
x
V

R

N

E
|
E

I

V

L

L

R

R

G

D

I

M

I

V

A

A

R
x
T

-

L

G

G

L

L

G

G

L

M

active site: Y126 (≠ M72), T127 (≠ S73), E241 (= E196), T376 (≠ R324)
binding flavin-adenine dinucleotide: I124 (= I70), Y126 (≠ M72), T127 (≠ S73), G132 (= G78), T133 (≠ S79), F157 (≠ W107), S159 (≠ T109), V359 (vs. gap), F362 (≠ L310), G363 (≠ R311), V366 (≠ T314), E368 (= E316)

4x28A Crystal structure of the chse4-chse5 complex from mycobacterium tuberculosis (see paper)
31% identity, 97% coverage: 4:322/329 of query aligns to 56:375/386 of 4x28A

query
sites
4x28A

P

P

H

K

Q

E

Y

Y

G

G

H

Q

D

G

A

R

S

S

P

A

W

M

A

D

R

Q

Y

L

V

I

V

F

I

T

E

D

E

E

L

A

A

I

A

A

G

G

A

A

P

P

V

V

S

P

A

-

H

-

W

F

I

L

A

T

D

I

R

N

Q

S

S

V

G

A

P

P

L

T

I

I

L

M

R

A

Y

Y

G

G

T

T

E

D

A

E

Q

Q

R

K

Q

R

K

F

Y

F

L

L

P

P

G

R

I

I

C

A

K

A

G

G

E

D

V

L

Y

H

F

F

C

S

I
|
I

G

G

M
x
Y

S
|
S

E

E

P

P

N

G

S

A

G
|
G

S
x
T

D

D

L

L

A

A

S

N

I

L

R

R

S

T

R

T

A

A

V

V

R

R

D

D

E

-

A

-

G

G

D

D

G

-

S

D

W

Y

R

V

L

V

S

N

G

G

Q

Q

K

K

V

M

W
|
W

T

T

T
x
S

N

L

A

I

H

Q

H

Y

A

A

Q

D

Y

Y

M

V

I

W

A

L

L

A

V

V

R

R

T

T

G

N

A

P

A

E

T

S

G

S

E

-

K

G

A

A

A

K

R

K

H

H

E

R

G

G

M

I

S

S

Q

V

F

L

I

I

I

V

D

P

L

T

K

T

A

A

A

E

G

G

V

F

T

S

V

W

R

T

P

P

I

V

R

H

D

T

L

M

A

A

G

G

G

P

E

D

H

T

F

-

N

S

E

A

V

T

Y

Y

L

Y

D

S

D

D

V

V

R

R

L

V

D

P

A

V

D

A

A

N

L

R

I

V

G

G

T

E

E

E

G

N

Q

A

G

G

W

W

E

K

Q

L

V

V

T

T

A

N

E

Q

L

L

A

N

F

H

E
|
E

R

R

S

V

G

A

P

L

E

V

R

S

F

P

L

A

S

P

S

I

M

F

A

G

L

C

L

L

H

R

T

E

L

V

I

R

D

E

A

W

V

A

G

Q

A

N

Q

T

P

K

D

D

A

A

-

G

-

T

-

R

-

L

L

I

Q

D

A

S

H

E

A

W

V

V

G

Q

R

L

L

N

S

L

A

A

R

R

L

V

V

H

V

A

L

K

R

A

Q

E

M

V

S

L

L

K

A

L

V

I

T

N

A

W

E

E

L

L

A

A

A

S

G

S

G

Q

N

G

P

P

A

S

W

P

A

A

-

D

A

A

S

S

C

A

L

A

K

K

D

V

L

F

G

G

A

T

A

E

F

L

E

A

Q

T

E

E

I

A

P

Y

E

R

L

L

A

L

Q

M

Q

E

V

V

L

L

D

G

V

T

A

A

P

A

A

T

V

L

G

R

G

Q

G

N

S

S

D

P

H

G

A

A

Q

L

V

L

L

R

A

G

A

R

L

V

M

E

Q

R

M

M

A

H

P

R

S

A

-

C

-

L

-

I

F

L

S

T

L
x
F

R

G

G
|
G

G

G

T
|
T

R

N

E
|
E

I
x
V

L

Q

R

R

G

D

I

I

active site: Y122 (≠ M72), S123 (= S73), E240 (= E196), G365 (= G312)
binding dihydroflavine-adenine dinucleotide: I120 (= I70), Y122 (≠ M72), S123 (= S73), G128 (= G78), T129 (≠ S79), W153 (= W107), S155 (≠ T109), F363 (≠ L310), T367 (= T314), E369 (= E316), V370 (≠ I317)

Sites not aligning to the query:

active site: 377

I6YCA3 Acyl-CoA dehydrogenase FadE26; ACAD; 3-oxocholest-4-en-26-oyl-CoA dehydrogenase alpha subunit; EC 1.3.99.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
31% identity, 97% coverage: 4:322/329 of query aligns to 63:388/400 of I6YCA3

query
sites
I6YCA3

P

P

H

K

Q

E

Y

Y

G

G

H

Q

D

G

A

R

S

S

P

A

W

M

A

D

R

Q

Y

L

V

I

V

F

I

T

E

D

E

E

L

A

A

I

A

A

G

G

A

A

P

P

V

V

S

P

A

-

H

-

W

F

I

L

A

T

D

I

R

N

Q

S

S

V

G

A

P

P

L

T

I

I

L

M

R

A

Y

Y

G

G

T

T

E

D

A

E

Q

Q

R

K

Q

R

K

F

Y

F

L

L

P

P

G

R

I

I

C

A

K

A

G

G

E

D

V

L

Y

H

F

F

C

S

I
|
I

G
|
G

M
x
Y

S
|
S

E

E

P

P

N

G

S

A

G

G

S
x
T

D

D

L

L

A

A

S

N

I

L

R

R

S

T

R

T

A

A

V

V

R

R

D

D

E

-

A

-

G

G

D

D

G

-

S

D

W

Y

R

V

L

V

S

N

G

G

Q

Q

K

K

V

M

W

W

T

T

T
x
S

N

L

A

I

H

Q

H

Y

A

A

Q

D

Y

Y

M

V

I

W

A

L

L

A

V

V

R

R

T

T

G

N

A

P

A

E

T

S

G

-

E

S

K

G

A

A

A

K

R

K

H

H

E

R

G

G

M

I

S

S

Q

V

F

L

I

I

I

V

D

P

L

T

K

T

A

A

A

E

G

G

V

F

T

S

V

W

R

T

P

P

I

V

R

H

D

T

L

M

A

A

G

G

G

P

E

D

H

T

F

-

N

S

E

A

V

T

Y

Y

L

Y

D

S

D

D

V

V

R

R

L

V

D

P

A

V

D

A

A

N

L

R

I

V

G

G

T

E

E

E

G

N

Q

A

G

G

W

W

E

K

Q

L

V

V

T

T

A

N

E

Q

L

L

A

N

F

H

E
|
E

R

R

-

V

-

A

-

L

S

V

G

S

P

P

E

A

R

P

F

I

L

F

S

G

S

C

M

L

A

R

L

E

L

V

H

R

T

E

-

W

-

A

-

Q

-

N

-

T

-

K

-

D

-

A

-

G

-

T

-

R

L

L

I

I

D

D

A

S

V

E

G

W

A

V

Q

Q

P

L

D

N

A

L

L

A

Q

R

A

V

H

H

A

A

V

-

G

-

R

-

L

-

S

K

A

A

R

E

L

V

V

L

V

K

L

L

R

I

Q

N

M

W

S

E

L

L

A

A

V

S

T

S

A

Q

E

S

-

G

-

P

L

K

A

D

A

A

G

G

G

P

N

S

P

P

A

A

W

-

A

D

A

A

S

S

C

A

L

A

K

K

D

V

L

F

G

G

A

T

A

E

F

L

E

A

Q

T

E

E

I

A

P

Y

E

R

L

L

A

L

Q

M

Q

E

V

V

L

L

D

G

V

T

A

A

P

A

A

T

V

L

G

R

G

Q

G

N

S

S

D

P

H

G

A

A

Q

L

V

L

L

R

A

G

A

R

L

V

M

E

Q

R

M

M

A

H

P

R

S

A

-

C

-

L

-

I

F

L

S

T

L

F

R

G

G

G

T
|
T

R
x
N

E
|
E

I

V

L

Q

R

R

G

D

I

I

IGYS 127:130 (≠ IGMS 70:73) binding
T136 (≠ S79) binding
S162 (≠ T109) binding
E247 (= E196) mutation to A: Loss of dehydrogenase activity.
TNE 380:382 (≠ TRE 314:316) binding

8i4rA Crystal structure of acyl-coa dehydrogenase complexed with acetyl-coa from thermobifida fusca
38% identity, 55% coverage: 4:185/329 of query aligns to 54:232/381 of 8i4rA

query
sites
8i4rA

P

P

H

H

-

V

-

A

-

E

Q

E

Y

Y

G

G

H

V

D

G

A

A

S

D

P

A

W

L

A

A

R

T

Y

C

V

I

V

V

I

I

E

E

L

I

L

A

A

R

A

V

G

C

A

A

P

S

V

S

S

S

A

L

H

I

W

P

I

A

A

V

D

N

R

K

Q

L

-

G

S

S

G

M

P

P

L

L

I

I

L

L

R

S

Y

-

G

G

T

S

E

D

A

E

Q

V

R

K

Q

Q

K

R

Y

Y

L

L

P

P

G

E

I

L

C

A

K

S

G

G

E

E

V

A

Y

M

F

F

C

S

I
x
Y

G

G

M
x
L

S
|
S

E

E

P

R

N

E

S

A

G
|
G

S
|
S

D

D

L
x
T

A

A

S

S

I

M

R

R

S

T

R

R

A

A

V

V

R

R

D

D

E

-

A

-

G

G

D

D

G

-

S

D

W

W

R

I

L

L

S

N

G

G

Q

Q

K

K

V

S

W
|
W

T
x
I

T
|
T

N

N

A

A

H

G

H

I

A

S

Q

K

Y

Y

M

Y

I

T

A

V

L

M

V

-

R

-

T

-

G

-

A

A

A

V

T

T

G

D

E

P

K

D

A

G

A

P

R

R

H

G

E
x
R

G

N

M

I

S

S

Q

A

F

F

I

V

D

H

L

I

K

D

A

D

A

P

G

G

V

F

T

S

V

F

-

G

R

E

P

P

I

E

R

R

D

K

L

L

A

G

-

I

G

K

G

G

E

S

H

P

F

T

N

R

E

E

V

L

Y

I

L

F

D

D

D

N

V

V

R

R

L

I

D

P

A

G

D

D

A

R

L

L

I

V

G

G

T

K

E

V

G

G

Q

E

G

G

binding acetyl coenzyme *a: S132 (= S79), T134 (≠ L81), R180 (≠ E135)
binding flavin-adenine dinucleotide: Y123 (≠ I70), L125 (≠ M72), S126 (= S73), G131 (= G78), S132 (= S79), W156 (= W107), I157 (≠ T108), T158 (= T109)

Sites not aligning to the query:

binding acetyl coenzyme *a: 234, 237, 238, 240, 241, 244, 365, 366, 377
binding flavin-adenine dinucleotide: 360, 367, 369

8i4pA Crystal structure of acyl-coa dehydrogenase from thermobifida fusca
38% identity, 55% coverage: 4:185/329 of query aligns to 54:232/381 of 8i4pA

query
sites
8i4pA

P

P

H

H

-

V

-

A

-

E

Q

E

Y

Y

G

G

H

V

D

G

A

A

S

D

P

A

W

L

A

A

R

T

Y

C

V

I

V

V

I

I

E

E

L

I

L

A

A

R

A

V

G

C

A

A

P

S

V

S

S

S

A

L

H

I

W

P

I

A

A

V

D

N

R

K

Q

L

-

G

S

S

G

M

P

P

L

L

I

I

L

L

R

S

Y

-

G

G

T

S

E

D

A

E

Q

V

R

K

Q

Q

K

R

Y

Y

L

L

P

P

G

E

I

L

C

A

K

S

G

G

E

E

V

A

Y

M

F

F

C

S

I
x
Y

G

G

M
x
L

S
|
S

E

E

P

R

N

E

S

A

G
|
G

S
|
S

D

D

L

T

A

A

S

S

I

M

R

R

S

T

R

R

A

A

V

V

R

R

D

D

E

-

A

-

G

G

D

D

G

-

S

D

W

W

R

I

L

L

S

N

G

G

Q

Q

K

K

V

S

W
|
W

T
x
I

T
|
T

N

N

A

A

H

G

H

I

A

S

Q

K

Y

Y

M

Y

I

T

A

V

L

M

V

-

R

-

T

-

G

-

A

A

A

V

T

T

G

D

E

P

K

D

A

G

A

P

R

R

H

G

E

R

G

N

M

I

S

S

Q

A

F

F

I

V

D

H

L

I

K

D

A

D

A

P

G

G

V

F

T

S

V

F

-

G

R

E

P

P

I

E

R

R

D

K

L

L

A

G

-

I

G

K

G

G

E

S

H

P

F

T

N

R

E

E

V

L

Y

I

L

F

D

D

D

N

V

V

R

R

L

I

D

P

A

G

D

D

A

R

L

L

I

V

G

G

T

K

E

V

G

G

Q

E

G

G

binding flavin-adenine dinucleotide: Y123 (≠ I70), L125 (≠ M72), S126 (= S73), G131 (= G78), S132 (= S79), W156 (= W107), I157 (≠ T108), T158 (= T109)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 360, 364, 367, 369

7w0jE Acyl-coa dehydrogenase, tfu_1647
38% identity, 55% coverage: 4:185/329 of query aligns to 55:233/382 of 7w0jE

query
sites
7w0jE

P

P

H

H

-

V

-

A

-

E

Q

E

Y

Y

G

G

H

V

D

G

A

A

S

D

P

A

W

L

A

A

R

T

Y

C

V

I

V

V

I

I

E

E

L

I

L

A

A

R

A

V

G

C

A

A

P

S

V

S

S

S

A

L

H

I

W

P

I

A

A

V

D

N

R

K

Q

L

-

G

S

S

G

M

P

P

L

L

I

I

L

L

R

S

Y

-

G

G

T

S

E

D

A

E

Q

V

R

K

Q

Q

K

R

Y

Y

L

L

P

P

G

E

I

L

C

A

K

S

G

G

E

E

V

A

Y

M

F

F

C

S

I

Y

G

G

M

L

S
|
S

E

E

P

R

N

E

S

A

G

G

S

S

D

D

L

T

A

A

S

S

I

M

R

R

S

T

R

R

A

A

V

V

R

R

D

D

E

-

A

-

G

G

D

D

G

-

S

D

W

W

R

I

L

L

S

N

G

G

Q

Q

K

K

V

S

W
|
W

T

I

T

T

N

N

A

A

H

G

H

I

A

S

Q

K

Y

Y

M

Y

I

T

A

V

L

M

V

-

R

-

T

-

G

-

A

A

A

V

T

T

G

D

E

P

K

D

A

G

A

P

R

R

H

G

E

R

G

N

M

I

S

S

Q

A

F

F

I

V

D

H

L

I

K

D

A

D

A

P

G

G

V

F

T

S

V

F

-

G

R

E

P

P

I

E

R

R

D

K

L

L

A

G

-

I

G

K

G

G

E

S

H

P

F

T

N

R

E

E

V

L

Y

I

L

F

D

D

D

N

V

V

R

R

L

I

D

P

A

G

D

D

A

R

L

L

I

V

G

G

T

K

E

V

G

G

Q

E

G

G

binding [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2R,3S,4S)-5-azanyl-2,3,4-tris(oxidanyl)pentyl] hydrogen phosphate: S127 (= S73), W157 (= W107)

Sites not aligning to the query:

binding [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2R,3S,4S)-5-azanyl-2,3,4-tris(oxidanyl)pentyl] hydrogen phosphate: 270, 272, 273, 277, 280, 283, 339, 340, 343, 365, 366, 368, 370, 371

2pg0A Crystal structure of acyl-coa dehydrogenase from geobacillus kaustophilus
27% identity, 98% coverage: 6:328/329 of query aligns to 58:380/380 of 2pg0A

query
sites
2pg0A

Q

K

Y

Y

G

G

H

L

D

N

A

A

S

D

P

F

W

A

A

Y

R

S

Y

V

V

V

V

I

I

N

E

E

L

L

L

E

A

K

A

V

G

G

A

S

P

S

V

L

S

V

A

G

H

I

W

G

I

L

A

H

D

N

R

D

Q

I

S

V

G

T

P

P

L

Y

I

I

L

A

R

S

Y

Y

G

G

T

T

E

E

A

E

Q

Q

R

K

Q

Q

K

K

Y

W

L

L

P

P

G

K

I

C

C

V

K

T

G

G

E

E

V

L

Y

I

F

T

C

A

I
|
I

G

A

M
|
M

S
x
T

E

E

P

P

N

G

S

A

G
|
G

S
|
S

D

D

L

L

A

A

S

N

I

I

R

S

S

T

R

T

A

A

V

V

R

K

D

D

E

-

A

-

G

G

D

D

G

-

S

Y

W

Y

R

I

L

V

S

N

G

G

Q

Q

K

K

V

T

W
x
F

T
x
I

T
|
T

N

N

A

G

H

I

H

H

A

A

Q

D

Y

L

M

I

I

V

A

V

L

A

V

C

R

K

T

T

G

D

A

P

A

-

T

-

G

-

E

Q

K

A

A

K

A

P

R

P

H

H

E

R

G

G

M

I

S

S

Q

L

F

L

I

V

D

E

L

R

K

D

A

T

A

P

G

G

V

F

T

T

V

R

R

G

P

R

I

K

R

L

D

E

L

K

A

V

G

G

-

L

-

H

G

A

E

Q

H

D

F

T

N

A

E

E

V

L

Y

F

L

F

D

Q

D

D

V

A

R

K

L

V

D

P

A

A

D

Y

A

N

L

L

I

L

G

G

T

E

E

E

G

G

Q

K

G

G

W

F

E

Y

Q

Y

V

L

T

M

A

E

E

K

L

L

A

Q

F

Q

E
|
E

R

R

-

L

-

V

-

A

-

I

-

A

-

Q

S

T

G

A

P

A

E

E

R

V

F

M

L

F

S

S

S

L

M

T

A

K

L

Q

L

Y

H

V

T

K

L

Q

I
x
R

D

T

A

A

V
x
F

G

G

A

K

Q

R

P

V

D

S

A

E

L
x
F

Q

Q

A

T

-

V

-

Q

H

F

A

R

V

L

G

A

R

E

L

M

S

A

A

T

R

E

L

I

V

A

V

L

L

G

R

R

Q

T

M

F

S

V

L

D

A

R

V

V

T

I

A

E

E

E

L

H

A

M

A

A

G

G

G

K

N

Q

P

I

A

V

W

T

A

E

A

V

S

S

C

M

L

A

K

K

D

-

L

-

G

-

A

-

F

-

E

-

Q

W

E

W

I

I

P

T

E

E

L

M

A

A

Q

K

Q

R

V

V

L

A

D

A

V

E

A

A

P

M

A
x
Q

V
x
L

G

H

G
|
G

S

Y

D

G

H

Y

A

M

Q

E

-

E

-

Y

V

E

L

I

A

A

A

R

L

R

M

Y

Q

R

M

D

A

I

P

P

S
x
V

F

S

S

A

L
x
I

R
x
Y

G
x
A

G

G

T
|
T

R

N

E
|
E

I
x
M

L

M

R

K

G

T

I

I

A

A

R
|
R

G

Q

L

L

G

D

L

L

active site: M124 (= M72), T125 (≠ S73), E243 (= E196), A364 (≠ G312), R376 (= R324)
binding flavin-adenine dinucleotide: I122 (= I70), M124 (= M72), T125 (≠ S73), G130 (= G78), S131 (= S79), F155 (≠ W107), I156 (≠ T108), T157 (= T109), R269 (≠ I214), F272 (≠ V217), F279 (≠ L224), Q337 (≠ A287), L338 (≠ V288), G340 (= G290), G341 (= G291), V359 (≠ S307), I362 (≠ L310), Y363 (≠ R311), T366 (= T314), E368 (= E316), M369 (≠ I317)

6es9A Methylsuccinyl-coa dehydrogenase of paracoccus denitrificans with bound flavin adenine dinucleotide (see paper)
34% identity, 57% coverage: 1:189/329 of query aligns to 210:401/545 of 6es9A

query
sites
6es9A

M

L

T

T

L

I

P

P

H

E

Q

E

Y

F

G

G

H

L

D

G

A

L

S

S

P

K

W

A

A

S

R

M

Y

V

V

V

I

T

E

E

L

L

L

S

A

R

A

G

G

Y

A

I

P

G

V

V

S

G

A

S

H

L

W

G

I

T

A

R

D

S

R

E

Q

I

S

A

G

A

P

E

L

L

I

I

L

L

R

C

Y

G

G

G

T

T

E

E

A

A

Q

Q

R

K

Q

A

K

K

Y

W

L

L

P

P

G

G

I

L

C

A

K

S

G

G

E

E

V

I

Y

L

F

S

C

T

I
x
A

G

V

M
x
F

S
x
T

E

E

P

P

N

N

S

T

G
|
G

S
|
S

D

D

L

L

A

G

S

S

I

L

R

R

S

T

R

R

A

A

V

V

R

R

D

D

E

-

A

-

G

G

D

E

G

-

S

D

W

W

R

V

L

V

S

T

G

G

Q

N

K

K

V

T

W
|
W

T
x
I

T
|
T

N

H

A

A

H

Q

H

R

A

T

Q

H

Y

V

M

M

I

T

A

L

L

L

V

A

R

R

T

T

G

D

A

P

A

E

T

T

G

T

E

D

K

-

A

-

R

-

H

W

E

R

G

G

M

L

S

S

Q

M

F

F

I

L

I

A

D

E

L

-

K

K

A

E

A

P

G

G

V

T

T

D

V

D

R

D

P

P

I

F

R

P

D

T

-

P

-

G

L

M

A

T

G

G

E

E

-

I

-

E

-

V

-

L

-

G

-

Y

-

R

-

G

-

M

H

K

F
x
E

N

Y

E

E

V

L

Y

G

L

F

D

D

D

G

V

F

R

R

L

I

D

K

A

G

D

E

A

N

L

L

I

L

G

G

T

G

E

E

-

P

G

G

Q

R

G

G

W

F

E

K

Q

Q

V

L

active site: F281 (≠ M72), T282 (≠ S73)
binding flavin-adenine dinucleotide: A279 (≠ I70), F281 (≠ M72), T282 (≠ S73), G287 (= G78), S288 (= S79), W312 (= W107), I313 (≠ T108), T314 (= T109), E374 (≠ F163)

Sites not aligning to the query:

active site: 408, 541
binding coenzyme a: 75, 467, 470
binding flavin-adenine dinucleotide: 50, 434, 436, 437, 441, 444, 502, 503, 505, 506, 528, 531, 533, 534

1ivhA Structure of human isovaleryl-coa dehydrogenase at 2.6 angstroms resolution: structural basis for substrate specificity (see paper)
27% identity, 99% coverage: 1:326/329 of query aligns to 58:384/387 of 1ivhA

query
sites
1ivhA

M

I

T

T

L

A

P

P

H

V

Q

Q

Y

Y

G

G

H

S

D

G

A

L

S

G

P

Y

W

L

A

E

R

H

Y

V

V

L

V

V

I

M

E

E

L

I

L

S

A

R

A

A

G

S

A

G

P

A

V

V

S

G

A

L

H

S

W

Y

I

G

A

A

D

H

R

S

Q

N

-

L

S

C

G

I

P

N

L

Q

I

L

L

V

R

R

Y

N

G

G

T

N

E

E

A

A

Q

Q

R

K

Q

E

K

K

Y

Y

L

L

P

P

G

K

I

L

C

I

K

S

G

G

E

E

V

Y

Y

I

F

G

C

A

I
x
L

G

A

M
|
M

S
|
S

E

E

P

P

N

N

S

A

G
|
G

S
|
S

D

D

L

V

A

V

S

S

I

M

R

K

S

L

R

K

A

A

V

-

R

-

D

-

E

E

A

K

G

G

D

N

G

-

S

H

W

Y

R

I

L

L

S

N

G

G

Q

N

K

K

V

F

W
|
W

T

I

T
|
T

N

N

A

G

H

P

H

D

A

A

Q

D

Y

V

M

L

I

I

A

V

L

Y

V

A

R

K

T

T

G

D

A

L

A

A

T

A

G

-

E

V

K

P

A

A

A
x
S

R
|
R

H

-

E

-

G

G

M

I

S

T

Q

A

F

F

I

I

I

V

D

E

L

K

K

G

A

M

A

P

G

G

V

F

T

S

V

T

R

S

P

K

I

K

R

L

D

D

L

K

A

L

G

G

-

M

-

R

G

G

E

S

H

N

F

T

N

C

E

E

V

L

Y

I

L

F

D

E

D

D

V

C

R

K

L

I

D

P

A

A

D

A

A

N

L

I

I

L

G

G

T

H

E

E

G

N

Q

K

G

G

W
x
V

E
x
Y

Q

V

V

L

T
x
M

A

S

E

G

L

L

A

D

F

L

E
|
E

R
|
R

-

L

-

V

-

L

-

A

S

G

G

G

P

P

E

L

R

G

F

L

L

M

S

Q

S

A

M

V

A

-

L

L

L

D

H

H

T

T

L

I

I

P

-

Y

-

L

-

H

-

V

-
x
R

D

E

A

A

V
x
F

G

G

A

Q

Q

K

P

I

D

G

A

H

L
x
F

Q

Q

A

L

H
x
M

-

Q

-

G

A

K

V

M

G

A

R

D

L

M

S

Y

A

T

R

R

L

L

V

M

V

A

L

C

R

R

Q

Q

M

Y

S

V

L

Y

A

N

V

V

T

A

A

K

E

A

L

C

A

D

A

E

G

G

G

H

N

C

P

T

A

A

W

-

A

-

S

-

C

-

L

-

K

K

D

D

L

C

G

A

A

G

A

V

F

I

E

L

Q

Y

E

S

I

A

P

E

E

C

L

A

A

T

Q

Q

Q

V

V

A

L

L

D

D

V

G

A

I

P
x
Q

A
x
C

V

F

G

G

G
|
G

-

N

G

G

S

Y

D

I

H

N

A

D

Q

F

V

P

L

M

A

G

A

R

L

F

M

L

Q

R

M

D

A

A

P

K

S

L

F

Y

S

E

L

I

R
x
G

G
x
A

G
|
G

T
|
T

R

S

E
|
E

I
x
V

L

R

R

R

G

L

I

V

I

I

A

G

R
|
R

G

A

L

F

active site: M130 (= M72), S131 (= S73), E249 (= E196), A370 (≠ G312), R382 (= R324)
binding coenzyme a persulfide: S137 (= S79), S185 (≠ A132), R186 (= R133), V239 (≠ W186), Y240 (≠ E187), M243 (≠ T190), E249 (= E196), R250 (= R197), G369 (≠ R311), A370 (≠ G312), G371 (= G313), V375 (≠ I317)
binding flavin-adenine dinucleotide: L128 (≠ I70), M130 (= M72), S131 (= S73), G136 (= G78), S137 (= S79), W161 (= W107), T163 (= T109), R275 (vs. gap), F278 (≠ V217), F285 (≠ L224), M288 (≠ H227), Q343 (≠ P286), C344 (≠ A287), G347 (= G290), T372 (= T314), E374 (= E316)

8sgrA Isovaleryl-CoA dehydrogenase, mitochondrial (see paper)
27% identity, 99% coverage: 1:326/329 of query aligns to 62:388/393 of 8sgrA

query
sites
8sgrA

M

I

T

T

L

A

P

P

H

V

Q

Q

Y

Y

G

G

H

S

D

G

A

L

S

G

P

Y

W

L

A

E

R

H

Y

V

V

L

V

V

I

M

E

E

L

I

L

S

A

R

A

A

G

S

A

G

P

A

V

V

S

G

A

L

H

S

W

Y

I

G

A

A

D

H

R

S

Q

N

-

L

S

C

G

I

P

N

L

Q

I

L

L

V

R

R

Y

N

G

G

T

N

E

E

A

A

Q

Q

R

K

Q

E

K

K

Y

Y

L

L

P

P

G

K

I

L

C

I

K

S

G

G

E

E

V

Y

Y

I

F

G

C

A

I

L

G

A

M

M

S
|
S

E

E

P

P

N

N

S

A

G
|
G

S
|
S

D

D

L

V

A

V

S

S

I

M

R

K

S

L

R

K

A

A

V

-

R

-

D

-

E

E

A

K

G

G

D

N

G

-

S

H

W

Y

R

I

L

L

S

N

G

G

Q

N

K

K

V

F

W
|
W

T

I

T
|
T

N

N

A

G

H

P

H

D

A

A

Q

D

Y

V

M

L

I

I

A

V

L

Y

V

A

R

K

T

T

G

D

A

L

A

A

T

A

G

-

E

V

K

P

A

A

A

S

R

R

H

-

E

-

G

G

M

I

S

T

Q

A

F

F

I

I

I

V

D

E

L

K

K

G

A

M

A

P

G

G

V

F

T

S

V

T

R

S

P

K

I

K

R

L

D

D

L

K

A

L

G

G

-

M

-

R

G

G

E

S

H

N

F

T

N

C

E

E

V

L

Y

I

L

F

D

E

D

D

V

C

R

K

L

I

D

P

A

A

D

A

A

N

L

I

I

L

G

G

T

H

E

E

G

N

Q

K

G

G

W

V

E

Y

Q

V

V

L

T

M

A

S

E

G

L

L

A

D

F

L

E

E

R

R

-

L

-

V

-

L

-

A

S

G

G

G

P

P

E

L

R

G

F

L

L

M

S

Q

S

A

M

V

A

-

L

L

L

D

H

H

T

T

L

I

I

P

-

Y

-

L

-

H

-

V

-
x
R

D

E

A

A

V
x
F

G

G

A

Q

Q

K

P
x
I

D

G

A

H

L
x
F

Q

Q

A

L

H

M

-

Q

-

G

A

K

V

M

G

A

R

D

L

M

S

Y

A

T

R

R

L

L

V

M

V

A

L

C

R

R

Q

Q

M

Y

S

V

L

Y

A

N

V

V

T

A

A

K

E

A

L

C

A

D

A

E

G

G

G

H

N

C

P

T

A

A

W

-

A

-

S

-

C

-

L

-

K

K

D

D

L

C

G

A

A

G

A

V

F

I

E

L

Q

Y

E

S

I

A

P

E

E

C

L

A

A

T

Q

Q

Q

V

V

A

L

L

D

D

V

G

A

I

P
x
Q

A
x
C

V

F

G

G

G
|
G

-

N

G

G

S

Y

D

I

H

N

A

D

Q

F

V

P

L

M

A

G

A

R

L

F

M

L

Q

R

M

D

A

A

P

K

S
x
L

F

Y

S

E

L

I

R

G

G

A

G
|
G

T
|
T

R

S

E

E

I

V

L

R

R

R

G
x
L

I

V

I

I

A

G

R

R

G

A

L

F

binding flavin-adenine dinucleotide: S135 (= S73), G140 (= G78), S141 (= S79), W165 (= W107), T167 (= T109), R279 (vs. gap), F282 (≠ V217), I286 (≠ P221), F289 (≠ L224), Q347 (≠ P286), C348 (≠ A287), G351 (= G290), L369 (≠ S307), G375 (= G313), T376 (= T314), L382 (≠ G320)

P26440 Isovaleryl-CoA dehydrogenase, mitochondrial; IVD; Butyryl-CoA dehydrogenase; EC 1.3.8.4; EC 1.3.8.1 from Homo sapiens (Human) (see 5 papers)
27% identity, 99% coverage: 1:326/329 of query aligns to 95:421/426 of P26440

query
sites
P26440

M

I

T

T

L

A

P

P

H

V

Q

Q

Y

Y

G

G

H

S

D

G

A

L

S

G

P

Y

W

L

A

E

R

H

Y

V

V

L

V

V

I

M

E

E

L

I

L

S

A

R

A

A

G

S

A

G

P

A

V

V

S

G

A

L

H

S

W

Y

I

G

A

A

D

H

R

S

Q

N

-

L

S

C

G

I

P

N

L

Q

I

L

L

V

R

R

Y

N

G

G

T

N

E

E

A

A

Q

Q

R

K

Q

E

K

K

Y

Y

L

L

P

P

G

K

I

L

C

I

K

S

G

G

E

E

V

Y

Y

I

F

G

C

A

I
x
L

G
x
A

M
|
M

S
|
S

E
|
E

P
|
P

N
|
N

S
x
A

G
|
G

S
|
S

D

D

L

V

A

V

S

S

I

M

R

K

S

L

R

K

A

A

V

-

R

-

D

-

E

E

A

K

G

G

D

N

G

-

S

H

W

Y

R

I

L

L

S

N

G

G

Q

N

K

K

V

F

W
|
W

T
x
I

T
|
T

N

N

A

G

H

P

H

D

A

A

Q

D

Y

V

M

L

I

I

A

V

L

Y

V

A

R

K

T

T

G

D

A

L

A

A

T

A

G

-

E

V

K

P

A

A

A
x
S

R
|
R

H

-

E

-

G

G

M

I

S

T

Q

A

F

F

I

I

I

V

D

E

L

K

K

G

A

M

A

P

G

G

V

F

T

S

V

T

R

S

P

K

I

K

R

L

D

D

L

K

A

L

G

G

-

M

-

R

G
|
G

E

S

H

N

F

T

N

C

E

E

V

L

Y

I

L

F

D

E

D

D

V

C

R

K

L

I

D

P

A

A

D

A

A

N

L

I

I

L

G

G

T

H

E

E

G

N

Q

K

G

G

W

V

E
x
Y

Q

V

V

L

T

M

A

S

E

G

L

L

A
x
D

F
x
L

E
|
E

R
|
R

-

L

-

V

-

L

-
x
A

S

G

G

G

P

P

E

L

R

G

F

L

L

M

S

Q

S

A

M

V

A

-

L

L

L

D

H

H

T

T

L

I

I

P

-

Y

-

L

-

H

-

V

-
x
R

D

E

A

A

V

F

G

G

A

Q

Q

K

P

I

D

G

A

H

L

F

Q
|
Q

A

L

H

M

-

Q

-

G

A

K

V

M

G

A

R

D

L

M

S

Y

A

T

R

R

L

L

V

M

V

A

L

C

R

R

Q

Q

M

Y

S

V

L

Y

A

N

V

V

T

A

A

K

E

A

L

C

A

D

A

E

G

G

G

H

N

C

P

T

A

A

W

-

A

-

S

-

C

-

L

-

K

K

D

D

L

C

G

A

A

G

A

V

F

I

E

L

Q

Y

E

S

I

A

P

E

E

C

L

A

A

T

Q

Q

Q

V

V

A

L

L

D

D

V

G

A
x
I

P
x
Q

A
x
C

V
x
F

G
|
G

-

N

G

G

S

Y

D

I

H

N

A

D

Q

F

V

P

L

M

A

G

A

R

L

F

M

L

Q
x
R

M

D

A

A

P

K

S

L

F
x
Y

S

E

L

I

R

G

G
x
A

G
|
G

T
|
T

R
x
S

E
|
E

I

V

L

R

R

R

G

L

I

V

I

I

A

G

R

R

G

A

L

F

165:174 (vs. 70:79, 70% identical) binding
S174 (= S79) binding
WIT 198:200 (≠ WTT 107:109) binding
SR 222:223 (≠ AR 132:133) binding
G250 (= G160) to A: in IVA; uncertain significance
Y277 (≠ E187) binding
DLER 284:287 (≠ AFER 194:197) binding
E286 (= E196) active site, Proton acceptor; mutation to D: Residual isovaleryl-CoA dehydrogenase activity.; mutation to G: Loss of isovaleryl-CoA dehydrogenase activity. Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 407-E.; mutation to Q: Loss of isovaleryl-CoA dehydrogenase activity.
A291 (vs. gap) to V: in IVA; uncertain significance; dbSNP:rs886042098
R312 (vs. gap) binding
Q323 (= Q225) binding
I379 (≠ A285) to T: in IVA; uncertain significance
QCFGG 380:384 (≠ PAVGG 286:290) binding
R398 (≠ Q303) to Q: in IVA; uncertain significance; dbSNP:rs1477527791
Y403 (≠ F308) to N: in IVA; uncertain significance
A407 (≠ G312) mutation to E: Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 286-D.
AG 407:408 (≠ GG 312:313) binding
TSE 409:411 (≠ TRE 314:316) binding

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion

3pfdC Crystal structure of an acyl-coa dehydrogenase from mycobacterium thermoresistibile bound to reduced flavin adenine dinucleotide solved by combined iodide ion sad mr (see paper)
35% identity, 59% coverage: 3:197/329 of query aligns to 47:234/369 of 3pfdC

query
sites
3pfdC

L

V

P

P

H

E

Q

E

Y

Y

G

G

H

Q

D

G

A

A

S

D

P

S

W

V

A

A

R

T

Y

C

V

I

V

V

I

I

E

E

L

V

L

-

A

-

A

A

G

R

A

V

P

D

V

C

S

S

A

A

H

S

W

L

I

I

-

P

A

A

D

V

R

N

Q

K

S

L

G

G

P

T

-

M

-

G

L

L

I

I

L

L

R

R

Y

-

G

G

T

S

E

E

A

E

Q

L

R

K

Q

K

K

Q

Y

V

L

L

P

P

G

A

I

V

C

A

K

S

G

G

E

E

V

A

Y

M

F

A

C

S

I
x
Y

G

A

M
x
L

S
|
S

E

E

P

R

N

E

S

A

G
|
G

S
|
S

D

D

L

A

A

A

S

S

I

M

R

R

S

T

R

R

A

A

V

V

R

A

D

D

E

-

A

-

G

G

D

D

G

-

S

D

W

W

R

I

L

L

S

N

G

G

Q

S

K

K

V

C

W
|
W

T
x
I

T
|
T

N

N

A

G

H

G

H

K

A

S

Q

T

Y

W

M

Y

I

T

A

V

L

M

V

A

R

V

T

T

G

D

A

P

A

D

T

K

G

G

E

A

K

N

A

-

R

-

H

-

E

-

G

G

M

I

S

S

Q

A

F

F

I

M

I

V

D

H

L

K

K

D

A

D

A

E

G

G

V

F

T

T

V

V

R

G

P

P

I

K

R

E

D

R

L

K

A

L

G

G

-

I

-

K

G

G

E

S

H

P

F

T

N

T

E

E

V

L

Y

Y

L

F

D

E

D

N

V

C

R

R

L

I

D

P

A

G

D

D

A

R

L

I

I

I

G

G

T

E

E

P

G

G

Q

T

G

G

W

F

E

K

Q

T

V

A

T

L

A

A

E

T

L

L

A

D

F

H

E
x
T

R

R

active site: L116 (≠ M72), S117 (= S73), T233 (≠ E196)
binding dihydroflavine-adenine dinucleotide: Y114 (≠ I70), L116 (≠ M72), S117 (= S73), G122 (= G78), S123 (= S79), W147 (= W107), I148 (≠ T108), T149 (= T109)

Sites not aligning to the query:

active site: 353, 365
binding dihydroflavine-adenine dinucleotide: 259, 262, 266, 269, 326, 327, 330, 348, 352, 355, 357

5lnxD Crystal structure of mmgc, an acyl-coa dehydrogenase from bacillus subtilis.
32% identity, 60% coverage: 1:199/329 of query aligns to 50:242/374 of 5lnxD

query
sites
5lnxD

M

I

T

P

L

V

P

P

H

E

Q

Q

Y

Y

G

G

H

A

D

G

A

A

S

D

P

V

W

V

A

S

R

Y

Y

I

V

L

V

A

I

I

E

H

E

E

L

I

-

S

-

R

-

I

-

S

L

A

A

A

A

V

G

G

A

V

P

I

V

L

S

S

A

V

H

H

W

-

I

-

A

T

D

S

R

V

Q

G

S

T

G

N

P

P

L

-

I

I

L

L

R

Y

Y

F

G

G

T

N

E

E

A

E

Q

Q

R

K

Q

M

K

K

Y

Y

L

I

P

P

G

N

I

L

C

A

K

S

G

G

E

D

V

H

Y

L

F

G

C

A

I

F

G

A

M
x
L

S
x
T

E

E

P

P

N

H

S

S

G
|
G

S
|
S

D

D

L

A

A

G

S

S

I

L

R

R

S

T

R

T

A

A

V

I

R

K

D

K

E

-

A

-

G

-

D

N

G

G

S

K

W

Y

R

L

L

L

S

N

G

G

Q

S

K

K

V

I

W
x
F

T

I

T
|
T

N

N

A

G

H

G

H

A

A

A

Q

D

Y

I

M

Y

I

I

A

-

L

-

V

-

R

-

T

T

G

F

A

A

A

L

T

T

G

A

E

P

K

D

A

Q

A

G

R

R

H

H

E

-

G

G

M

I

S

S

Q

A

F

F

I

I

I

V

D

E

L

K

K

N

A

T

A

P

G

G

V

F

T

T

V

V

-

G

R

K

P

K

I

E

R

R

D

K

L

L

A

G

-

L

G

Y

G

G

E

S

H

N

F

T

N

T

E

E

V

L

Y

I

L

F

D

D

D

N

V

A

R

E

L

V

D

P

A

E

D

A

A

N

L

L

I

L

G

G

T

K

E

E

G

G

Q

D

G

G

W

F

E

H

Q

I

V

A

T

M

A

A

E

N

L

L

A

N

F

V

E
x
G

R

R

S

I

G

G

active site: L122 (≠ M72), T123 (≠ S73), G239 (≠ E196)
binding flavin-adenine dinucleotide: L122 (≠ M72), T123 (≠ S73), G128 (= G78), S129 (= S79), F153 (≠ W107), T155 (= T109)

Sites not aligning to the query:

active site: 358, 370
binding flavin-adenine dinucleotide: 265, 267, 268, 272, 275, 278, 331, 332, 335, 357, 360, 362

P50544 Very long-chain specific acyl-CoA dehydrogenase, mitochondrial; MVLCAD; VLCAD; EC 1.3.8.9 from Mus musculus (Mouse) (see paper)
32% identity, 43% coverage: 45:187/329 of query aligns to 190:332/656 of P50544

query
sites
P50544

I

I

L

L

R

L

Y

Y

G

G

T

T

E

K

A

A

Q

Q

R

R

Q

E

K

K

Y

Y

L

L

P

P

G

R

I

V

C

A

K

S

G

G

E

Q

V

A

Y

L

F

A

C

A

I

F

G

C

M

L

S

T

E

E

P

P

N

S

S

S

G

G

S

S

D

D

L

V

A

A

S

S

I

I

R

R

S

S

R

S

A

A

V

I

R

P

D

S

E

P

A
x
C

A

G

G

-

D

-

G

K

S

Y

W

Y

R

T

L

L

S

N

G

G

Q

S

K

K

V

I

W

W

T

I

T

S

N

N

A

G

H

G

H

L

A

A

Q

D

Y

I

M

F

I

T

A

V

L

F

V

A

R

K

T

T

G

P

A

I

A

K

T

D

G

A

E

A

K

T

A

G

A

A

R

V

H

K

E

E

G

K

M

I

S

T

Q

A

F

F

I

V

D

E

L

R

K

S

A

F

A

G

G

G

V

V

T

T

V

H

-

G

R

L

P

P

I

E

R

K

D

K

L

M

A

G

-

I

G

K

G

A

E

S

H

N

F

T

N

S

E

E

V

V

Y

Y

L

F

D

D

D

G

V

V

R

K

L

V

D

P

A

S

D

E

A

N

L

V

I

L

G

G

T

E

E

V

G

G

Q

D

G

G

W

F

E

K

C238 (≠ A93) modified: S-nitrosocysteine

P49748 Very long-chain specific acyl-CoA dehydrogenase, mitochondrial; VLCAD; EC 1.3.8.9 from Homo sapiens (Human) (see 8 papers)
30% identity, 57% coverage: 1:187/329 of query aligns to 144:331/655 of P49748

query
sites
P49748

M

L

T

Q

L

V

P

P

H

S

Q

E

Y

L

G

G

H

V

D

G

-

L

-

C

A

N

S

T

P

Q

W

Y

A

A

R

R

Y

L

V

V

V

E

I

I

E

V

E

G

L

M

-

H

-

D

L

L

A

G

A

V

G

G

A

I

P

T

V

L

S

G

A

A

H

H

W

Q

I

S

A

I

D

G

R

F

Q

K

S

G

G

-

P

-

L

-

I

I

L

L

R

L

Y

F

G

G

T

T

E

K

A

A

Q

Q

R

K

Q

E

K

K

Y

Y

L

L

P

P

G

K

I

L

C

A

K

S

G

G

E

E

V

T

Y

V

F

A

C

A

I
x
F

G
x
C

M
x
L

S
x
T

E
|
E

P
|
P

N
x
S

S
|
S

G
|
G

S
|
S

D

D

L

A

A

A

S

S

I

I

R

R

S

T

R

S

A

A

V

V

R

P

D

S

E

P

A

C

A

G

G

-

D

-

G

K

S

Y

W

Y

R

T

L

L

S

N

G

G

Q

S

K

K

V

L

W
|
W

T
x
I

T
x
S

N

N

A

G

H

G

H

L

A

A

Q

D

Y

I

M

F

I

T

A

V

L

F

V

A

R

K

T

T

G

P

A

V

A

T

T

D

G

P

E

A

K

T

A

G

A

A

R

V

H

K

E

E

G

K

M

I

S

T

Q

A

F

F

I

V

D

E

L

R

K

G

A

F

A

G

G

G

V

I

T

T

V

H

R

G

P

P

I

P

R

E

D

K

L

K

A

M

G

G

G

I

E

K

H

A

F

S

N

N

-

T

-

A

E

E

V

V

Y

F

L

F

D

D

D

G

V

V

R

R

L

V

D

P

A

S

D

E

A

N

L

V

I

L

G

G

T

E

E

V

G

G

Q

S

G

G

W

F

E

K

214:223 (vs. 70:79, 50% identical) binding
WIS 249:251 (≠ WTT 107:109) binding

Sites not aligning to the query:

1:40 modified: transit peptide, Mitochondrion
458 F → L: in ACADVLD; loss of acyl-CoA dehydrogenase activity; Loss of FAD cofactor-binding; dbSNP:rs118204017; F→T: Decreased acyl-CoA dehydrogenase activity. Decreased affinity for acyl-CoA. No effect on FAD cofactor-binding.; F→V: Loss of acyl-CoA dehydrogenase activity. Loss of FAD cofactor-binding.; F→Y: Decreased acyl-CoA dehydrogenase activity. No effect on affinity for acyl-CoA. Decreased FAD cofactor-binding.
461:463 binding
462 active site, Proton acceptor; E→D: Decreased acyl-CoA dehydrogenase activity. No effect on affinity for acyl-CoA. No effect on FAD cofactor-binding.; E→Q: Loss of acyl-CoA dehydrogenase activity. No effect on FAD cofactor-binding.
464:466 binding
490 A → P: in ACADVLD; decreased association with mitochondrial inner membrane; may affect substrate specificity, possibly reducing the affinity for long-chain acyl-CoA substrates; dbSNP:rs759775666; mutation A->G,V,S,D,H: Changed substrate specificity with decreased affinity for tetradecanoyl-CoA and hexadecanoyl-CoA.
502 L → P: in ACADVLD; decreased association with mitochondrial inner membrane; decreased specific activity towards several substrates in vitro
534 E → K: in ACADVLD; uncertain significance; dbSNP:rs2230180
562 binding
583 S → W: in ACADVLD; Loss of homodimerization; loss of localization to mitochondrial inner membrane; dbSNP:rs1085307648

8ca1B Cryo-em structure of the acadvl dimer from mus musculus. (see paper)
32% identity, 43% coverage: 45:187/329 of query aligns to 123:265/589 of 8ca1B

query
sites
8ca1B

I

I

L

L

R

L

Y

Y

G

G

T

T

E

K

A

A

Q

Q

R

R

Q

E

K

K

Y

Y

L

L

P

P

G

R

I

V

C

A

K

S

G

G

E

Q

V

A

Y

L

F

A

C

A

I
x
F

G

C

M

L

S
x
T

E

E

P

P

N

S

S

S

G
|
G

S
|
S

D

D

L

V

A

A

S

S

I

I

R

R

S

S

R

S

A

A

V

I

R

P

D

S

E

P

A

C

A

G

G

-

D

-

G

K

S

Y

W

Y

R

T

L

L

S

N

G

G

Q

S

K

K

V

I

W
|
W

T

I

T
x
S

N

N

A

G

H

G

H

L

A

A

Q

D

Y

I

M

F

I

T

A

V

L

F

V

A

R

K

T

T

G

P

A

I

A

K

T

D

G

A

E

A

K

T

A

G

A

A

R

V

H

K

E

E

G

K

M

I

S

T

Q

A

F

F

I

V

D

E

L

R

K

S

A

F

A

G

G

G

V

V

T

T

V

H

-

G

R

L

P

P

I

E

R

K

D

K

L

M

A

G

-

I

G

K

G

A

E

S

H

N

F

T

N

S

E

E

V

V

Y

Y

L

F

D

D

D

G

V

V

R

K

L

V

D

P

A

S

D

E

A

N

L

V

I

L

G

G

T

E

E

V

G

G

Q

D

G

G

W

F

E

K

binding flavin-adenine dinucleotide: F148 (≠ I70), T151 (≠ S73), G156 (= G78), S157 (= S79), W183 (= W107), S185 (≠ T109)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 300, 302, 303, 307, 312, 313, 369, 370, 395, 400, 401

Query Sequence

>Ac3H11_2717 FitnessBrowser__acidovorax_3H11:Ac3H11_2717
MTLPHQYGGHDASPWARYVVIEELLAAGAPVSAHWIADRQSGPLILRYGTEAQRQKYLPG
ICKGEVYFCIGMSEPNSGSDLASIRSRAVRDEAAGDGSWRLSGQKVWTTNAHHAQYMIAL
VRTGAATGEKAARHEGMSQFIIDLKAAGVTVRPIRDLAGGEHFNEVYLDDVRLDADALIG
TEGQGWEQVTAELAFERSGPERFLSSMALLHTLIDAVGAQPDALQAHAVGRLSARLVVLR
QMSLAVTAELAAGGNPAWAASCLKDLGAAFEQEIPELAQQVLDVAPAVGGGSDHAQVLAA
LMQMAPSFSLRGGTREILRGIIARGLGLR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory