SitesBLAST
Comparing Ac3H11_3014 FitnessBrowser__acidovorax_3H11:Ac3H11_3014 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
28% identity, 100% coverage: 1:261/261 of query aligns to 1:258/259 of 5zaiC
- active site: A65 (= A65), F70 (≠ M70), S82 (≠ A85), R86 (≠ A89), G110 (= G113), E113 (≠ G116), P132 (≠ S135), E133 (= E136), I138 (≠ L141), P140 (= P143), G141 (≠ A144), A226 (≠ I228), F236 (≠ R239)
- binding coenzyme a: K24 (≠ V24), L25 (≠ R25), A63 (= A63), G64 (= G64), A65 (= A65), D66 (≠ N66), I67 (≠ L67), P132 (≠ S135), R166 (= R168), F248 (= F251), K251 (= K254)
Q5HH38 1,4-dihydroxy-2-naphthoyl-CoA synthase; DHNA-CoA synthase; EC 4.1.3.36 from Staphylococcus aureus (strain COL) (see paper)
32% identity, 95% coverage: 13:259/261 of query aligns to 22:266/273 of Q5HH38
- R34 (= R25) binding in other chain
- SGGDQ 73:77 (≠ AGANL 63:67) binding in other chain
- S149 (≠ L141) binding in other chain
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
32% identity, 99% coverage: 1:259/261 of query aligns to 1:254/255 of 3q0jC
- active site: A65 (= A65), M70 (= M70), T80 (≠ R78), F84 (≠ I82), G108 (= G113), E111 (≠ G116), P130 (≠ S135), E131 (= E136), V136 (≠ L141), P138 (= P143), G139 (≠ A144), L224 (≠ I228), F234 (≠ R239)
- binding acetoacetyl-coenzyme a: Q23 (≠ E23), A24 (≠ V24), L25 (≠ R25), A27 (= A27), A63 (= A63), G64 (= G64), A65 (= A65), D66 (≠ N66), I67 (≠ L67), K68 (≠ N68), M70 (= M70), F84 (≠ I82), G107 (≠ A112), G108 (= G113), E111 (≠ G116), P130 (≠ S135), E131 (= E136), P138 (= P143), G139 (≠ A144), M140 (≠ T145)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
32% identity, 99% coverage: 1:259/261 of query aligns to 1:254/255 of 3q0gC
- active site: A65 (= A65), M70 (= M70), T80 (≠ R78), F84 (≠ I82), G108 (= G113), E111 (≠ G116), P130 (≠ S135), E131 (= E136), V136 (≠ L141), P138 (= P143), G139 (≠ A144), L224 (≠ I228), F234 (≠ R239)
- binding coenzyme a: L25 (≠ R25), A63 (= A63), I67 (≠ L67), K68 (≠ N68), Y104 (≠ D109), P130 (≠ S135), E131 (= E136), L134 (= L139)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
34% identity, 94% coverage: 14:259/261 of query aligns to 13:249/250 of 3q0gD
- active site: A64 (= A65), M69 (= M70), T75 (≠ R78), F79 (≠ I82), G103 (= G113), E106 (≠ G116), P125 (≠ S135), E126 (= E136), V131 (≠ L141), P133 (= P143), G134 (≠ A144), L219 (≠ I228), F229 (≠ R239)
- binding Butyryl Coenzyme A: F225 (≠ I235), F241 (= F251)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
33% identity, 94% coverage: 14:259/261 of query aligns to 13:253/256 of 3h81A
- active site: A64 (= A65), M69 (= M70), T79 (≠ R78), F83 (≠ I82), G107 (= G113), E110 (≠ G116), P129 (≠ S135), E130 (= E136), V135 (≠ L141), P137 (= P143), G138 (≠ A144), L223 (≠ I228), F233 (≠ R239)
- binding calcium ion: F233 (≠ R239), Q238 (≠ G244)
2uzfA Crystal structure of staphylococcus aureus 1,4-dihydroxy-2-naphthoyl coa synthase (menb) in complex with acetoacetyl coa (see paper)
32% identity, 95% coverage: 13:259/261 of query aligns to 17:253/260 of 2uzfA
- active site: G70 (≠ A65), R80 (≠ E79), L84 (≠ A83), G108 (= G113), V111 (≠ G116), T130 (≠ S135), G131 (≠ E136), S136 (≠ L141), D138 (≠ P143), A139 (= A144), A225 (≠ T231), Y233 (≠ R239)
- binding acetoacetyl-coenzyme a: V28 (= V24), R29 (= R25), S68 (≠ A63), G69 (= G64), G70 (≠ A65), D71 (≠ N66), Y104 (≠ D109), G108 (= G113)
Q7CQ56 1,4-dihydroxy-2-naphthoyl-CoA synthase; DHNA-CoA synthase; EC 4.1.3.36 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
31% identity, 95% coverage: 13:259/261 of query aligns to 33:278/285 of Q7CQ56
Q4WF54 Mevalonyl-coenzyme A hydratase sidH; Siderophore biosynthesis protein H; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
33% identity, 93% coverage: 17:259/261 of query aligns to 24:265/270 of Q4WF54
Sites not aligning to the query:
- 268:270 PTS1-type peroxisomal targeting signal
4i52A Scmenb im complex with 1-hydroxy-2-naphthoyl-coa (see paper)
30% identity, 96% coverage: 10:259/261 of query aligns to 16:268/275 of 4i52A
- active site: G77 (≠ A65), R82 (= R71), Y87 (= Y76), R95 (≠ D84), L99 (= L88), G123 (= G113), V126 (≠ G116), G146 (≠ E136), S151 (≠ L141), D153 (≠ P143), G154 (≠ A144), A240 (≠ T231), Y248 (≠ R239)
- binding 1-hydroxy-2-naphthoyl-CoA: H29 (≠ E23), K30 (≠ V24), R31 (= R25), A33 (= A27), S75 (≠ A63), G76 (= G64), G77 (≠ A65), D78 (≠ N66), Q79 (≠ L67), L96 (≠ A85), V98 (≠ K87), Y119 (≠ D109), I121 (≠ Y111), G123 (= G113), T145 (≠ S135), V149 (≠ L139), S151 (≠ L141), F152 (≠ I142)
4i4zA Synechocystis sp. Pcc 6803 1,4-dihydroxy-2-naphthoyl-coenzyme a synthase (menb) in complex with salicylyl-coa (see paper)
30% identity, 96% coverage: 10:259/261 of query aligns to 16:268/275 of 4i4zA
- active site: G77 (≠ A65), R82 (= R71), Y87 (= Y76), R95 (≠ D84), L99 (= L88), G123 (= G113), V126 (≠ G116), G146 (≠ E136), S151 (≠ L141), D153 (≠ P143), G154 (≠ A144), A240 (≠ T231), Y248 (≠ R239)
- binding Salicylyl CoA: H29 (≠ E23), K30 (≠ V24), R31 (= R25), S75 (≠ A63), G76 (= G64), G77 (≠ A65), D78 (≠ N66), Q79 (≠ L67), Y87 (= Y76), V98 (≠ K87), G123 (= G113), T145 (≠ S135), V149 (≠ L139), S151 (≠ L141), F260 (= F251), K263 (= K254)
- binding bicarbonate ion: G122 (≠ A112), Q144 (≠ L134), T145 (≠ S135), G146 (≠ E136), W174 (≠ F163)
3t88A Crystal structure of escherichia coli menb in complex with substrate analogue, osb-ncoa (see paper)
30% identity, 95% coverage: 13:259/261 of query aligns to 29:274/281 of 3t88A
- active site: G82 (≠ A65), R87 (≠ M70), Y93 (= Y76), H101 (≠ D84), L105 (= L88), G129 (= G113), V132 (≠ G116), G152 (≠ E136), S157 (≠ L141), D159 (≠ P143), G160 (≠ A144), A246 (≠ T231), Y254 (≠ R239)
- binding o-succinylbenzoyl-N-coenzyme A: Q39 (≠ E23), V40 (= V24), R41 (= R25), A43 (= A27), S80 (≠ A63), G81 (= G64), G82 (≠ A65), D83 (≠ N66), Q84 (≠ L67), K85 (≠ N68), Y93 (= Y76), V104 (≠ K87), L105 (= L88), Y125 (≠ D109), G129 (= G113), T151 (≠ S135), V155 (≠ L139), F158 (≠ I142), D159 (≠ P143), T250 (≠ I235), Y254 (≠ R239), F266 (= F251), K269 (= K254)
4i42A E.Coli. 1,4-dihydroxy-2-naphthoyl coenzyme a synthase (ecmenb) in complex with 1-hydroxy-2-naphthoyl-coa (see paper)
30% identity, 95% coverage: 13:259/261 of query aligns to 33:278/285 of 4i42A
- active site: G86 (≠ A65), R91 (≠ M70), Y97 (= Y76), H105 (≠ D84), L109 (= L88), G133 (= G113), V136 (≠ G116), G156 (≠ E136), S161 (≠ L141), D163 (≠ P143), G164 (≠ A144), A250 (≠ T231), Y258 (≠ R239)
- binding 1-hydroxy-2-naphthoyl-CoA: V44 (= V24), R45 (= R25), S84 (≠ A63), G85 (= G64), G86 (≠ A65), D87 (≠ N66), Q88 (≠ L67), K89 (≠ N68), Y97 (= Y76), V108 (≠ K87), Y129 (≠ D109), G133 (= G113), T155 (≠ S135), S161 (≠ L141), T254 (≠ I235), F270 (= F251), K273 (= K254)
P0ABU0 1,4-dihydroxy-2-naphthoyl-CoA synthase; DHNA-CoA synthase; EC 4.1.3.36 from Escherichia coli (strain K12) (see 4 papers)
30% identity, 95% coverage: 13:259/261 of query aligns to 33:278/285 of P0ABU0
- R45 (= R25) binding in other chain
- SGGDQK 84:89 (≠ AGANLN 63:68) binding in other chain
- K89 (≠ N68) mutation to A: Strongly decreases affinity for substrate and DHNA-CoA synthase activity.
- R91 (≠ M70) mutation to A: Loss of DHNA-CoA synthase activity.
- Y97 (= Y76) binding in other chain; mutation to F: Loss of DHNA-CoA synthase activity.
- YSIGG 129:133 (≠ DVYAG 109:113) binding in other chain
- Q154 (≠ L134) mutation to A: Reduces the specific DHNA-CoA synthase activity by 15-fold, whereas its affinity for hydrogencarbonate is reduced by 36-fold.
- QTG 154:156 (≠ LSE 134:136) binding
- T155 (≠ S135) binding in other chain
- G156 (≠ E136) mutation to D: Loss of DHNA-CoA synthase activity.
- S161 (≠ L141) binding in other chain
- W184 (≠ F163) mutation to F: Reduces the specific DHNA-CoA synthase activity by 530-fold, whereas its affinity for hydrogencarbonate is reduced by 20-fold.
- Y258 (≠ R239) binding
- R267 (≠ V248) mutation to A: Strongly decreases affinity for substrate and DHNA-CoA synthase activity.
- F270 (= F251) mutation to A: Strongly decreases affinity for substrate and DHNA-CoA synthase activity.
- K273 (= K254) binding ; mutation to A: Impairs protein folding.
3h02A 2.15 angstrom resolution crystal structure of naphthoate synthase from salmonella typhimurium.
31% identity, 95% coverage: 13:259/261 of query aligns to 29:259/266 of 3h02A
- active site: G82 (≠ A65), H86 (≠ W69), L90 (≠ A83), G114 (= G113), V117 (≠ G116), G137 (≠ E136), S142 (≠ L141), D144 (≠ P143), G145 (≠ A144), A231 (≠ T231), Y239 (≠ R239)
- binding bicarbonate ion: G113 (≠ A112), Q135 (≠ L134), G137 (≠ E136), W165 (≠ F163)
4elxA Structure of apo e.Coli. 1,4-dihydroxy-2- naphthoyl coa synthases with cl (see paper)
29% identity, 95% coverage: 13:259/261 of query aligns to 30:261/268 of 4elxA
- active site: G83 (≠ A65), H88 (≠ M70), L92 (≠ A74), G116 (= G113), V119 (≠ G116), G139 (≠ E136), S144 (≠ L141), D146 (≠ P143), G147 (≠ A144), A233 (≠ T231), Y241 (≠ R239)
- binding chloride ion: G115 (≠ A112), G139 (≠ E136), W167 (≠ F163)
4emlA Synechocystis sp. Pcc 6803 1,4-dihydroxy-2-naphthoyl-coenzyme a synthase (menb) in complex with bicarbonate (see paper)
30% identity, 96% coverage: 10:259/261 of query aligns to 16:254/261 of 4emlA
- active site: G77 (≠ A65), R81 (≠ W69), L85 (≠ A83), G109 (= G113), V112 (≠ G116), G132 (≠ E136), S137 (≠ L141), D139 (≠ P143), G140 (≠ A144), A226 (≠ T231), Y234 (≠ R239)
- binding bicarbonate ion: G108 (≠ A112), Q130 (≠ L134), G132 (≠ E136), W160 (≠ F163)
- binding chloride ion: D184 (= D187), R185 (≠ Q188), E187 (≠ D190), E188 (≠ A191)
4elwA Structure of e. Coli. 1,4-dihydroxy-2- naphthoyl coenzyme a synthases (menb) in complex with nitrate (see paper)
30% identity, 95% coverage: 13:259/261 of query aligns to 30:260/267 of 4elwA
- active site: G83 (≠ A65), L91 (≠ A83), G115 (= G113), V118 (≠ G116), G138 (≠ E136), S143 (≠ L141), D145 (≠ P143), G146 (≠ A144), A232 (≠ T231), Y240 (≠ R239)
- binding nitrate ion: G114 (≠ A112), T137 (≠ S135), G138 (≠ E136), F144 (≠ I142), W166 (≠ F163)
7xwvA Feruloyl-coa hydratase/lyase complexed with vanillin and coenzyme a (see paper)
33% identity, 79% coverage: 14:220/261 of query aligns to 15:223/244 of 7xwvA
- binding coenzyme a: T24 (≠ E23), K25 (≠ V24), R26 (= R25), A64 (= A63), G65 (= G64), M66 (≠ A65), D67 (≠ N66), L68 (= L67), W111 (≠ D109), F113 (≠ Y111), G114 (≠ A112), G115 (= G113), S137 (= S135)
- binding 4-hydroxy-3-methoxybenzaldehyde: M66 (≠ A65), Y71 (= Y76), F72 (≠ T77), E138 (= E136), G146 (≠ A144), G147 (≠ T145)
7xwtB Crystal structure of feruoyl-coa hydratase/lyase complexed with coa from sphingomonas paucimobilis (see paper)
33% identity, 79% coverage: 14:220/261 of query aligns to 16:224/277 of 7xwtB
- binding acetyl coenzyme *a: T25 (≠ E23), K26 (≠ V24), R27 (= R25), A29 (= A27), A65 (= A63), G66 (= G64), M67 (≠ A65), D68 (≠ N66), L69 (= L67), F73 (≠ T77), F114 (≠ Y111), G116 (= G113), S138 (= S135), W142 (≠ L139)
Query Sequence
>Ac3H11_3014 FitnessBrowser__acidovorax_3H11:Ac3H11_3014
MSSQNLSITQSGAVARITLTQPEVRNAFSDEVIAEITAAFTDVGSRPDVRAVVLAAEGPA
FCAGANLNWMRRMADYTREENIADAGKLAEMLRVIYECPKPTVARVQGDVYAGGMGLVAA
CDMAVAVDTAGFCLSEVKLGLIPATISPYVIRAMGARAAHRYFLTAERFDAAEALRIGFV
HTVVAADQLDAKVDELLKALTSASPNAVRACKKLVLQVAEREINAGLIAATVQGIADIRA
SDEGKEGVQSFLNKRKPNWLG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory