SitesBLAST
Comparing Ac3H11_3083 FitnessBrowser__acidovorax_3H11:Ac3H11_3083 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1gawB Crystal structure analysis of the ferredoxin-NADP+ reductase from maize leaf (see paper)
36% identity, 66% coverage: 143:422/422 of query aligns to 20:305/305 of 1gawB
- active site: Y86 (= Y210), S87 (= S211), C263 (= C380), E303 (= E420), Y305 (= Y422)
- binding flavin-adenine dinucleotide: R84 (= R208), L85 (≠ Q209), Y86 (= Y210), S87 (= S211), C105 (≠ T229), Y111 (≠ T235), G121 (= G244), V122 (≠ I245), C123 (≠ A246), S124 (= S247), T163 (= T286), Y305 (= Y422)
3vo2A Crystal structure of zea mays leaf ferredoxin-NADP+ reductase iii (see paper)
36% identity, 66% coverage: 143:422/422 of query aligns to 11:296/296 of 3vo2A
- active site: Y77 (= Y210), S78 (= S211), C254 (= C380), E294 (= E420), Y296 (= Y422)
- binding flavin-adenine dinucleotide: R75 (= R208), L76 (≠ Q209), Y77 (= Y210), S78 (= S211), C96 (≠ T229), V97 (= V230), L100 (vs. gap), Y102 (≠ T234), G112 (= G244), V113 (≠ I245), C114 (≠ A246), S115 (= S247), T154 (= T286), E294 (= E420), Y296 (= Y422)
1ewyA Anabaena pcc7119 ferredoxin:ferredoxin-NADP+-reductase complex (see paper)
34% identity, 66% coverage: 143:422/422 of query aligns to 12:303/303 of 1ewyA
- active site: Y79 (= Y210), S80 (= S211), C261 (= C380), E301 (= E420), Y303 (= Y422)
- binding flavin-adenine dinucleotide: R77 (= R208), L78 (≠ Q209), Y79 (= Y210), S80 (= S211), C98 (≠ T229), L102 (≠ V233), Y104 (≠ T235), G115 (= G244), V116 (≠ I245), C117 (≠ A246), S118 (= S247), E301 (= E420), Y303 (= Y422)
1queA X-ray structure of the ferredoxin:nadp+ reductase from the cyanobacterium anabaena pcc 7119 at 1.8 angstroms (see paper)
34% identity, 66% coverage: 143:422/422 of query aligns to 12:303/303 of 1queA
- active site: Y79 (= Y210), S80 (= S211), C261 (= C380), E301 (= E420), Y303 (= Y422)
- binding flavin-adenine dinucleotide: R77 (= R208), L78 (≠ Q209), Y79 (= Y210), S80 (= S211), C98 (≠ T229), L102 (≠ V233), Y104 (≠ T235), K105 (≠ D236), G115 (= G244), V116 (≠ I245), C117 (≠ A246), S118 (= S247), T157 (= T286), E301 (= E420), Y303 (= Y422)
- binding sulfate ion: S223 (= S345), R224 (= R346), R233 (≠ K352), Y235 (= Y354)
1gjrA Ferredoxin-NADP+ reductase complexed with NADP+ by cocrystallization (see paper)
34% identity, 66% coverage: 143:422/422 of query aligns to 4:295/295 of 1gjrA
- active site: Y71 (= Y210), S72 (= S211), C253 (= C380), E293 (= E420), Y295 (= Y422)
- binding flavin-adenine dinucleotide: R69 (= R208), L70 (≠ Q209), Y71 (= Y210), S72 (= S211), C90 (≠ T229), L94 (≠ V233), Y96 (≠ T235), G107 (= G244), V108 (≠ I245), C109 (≠ A246), S110 (= S247), T149 (= T286), E293 (= E420), Y295 (= Y422)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R92 (≠ K231), L94 (≠ V233), T147 (= T284), G148 (= G285), T149 (= T286), P186 (≠ R319), S215 (= S345), R216 (= R346), R225 (≠ K352), Y227 (= Y354), Q229 (= Q356), G254 (= G381), L255 (= L382), Y295 (= Y422)
2b5oA Ferredoxin-NADP reductase
34% identity, 66% coverage: 143:422/422 of query aligns to 4:292/292 of 2b5oA
- active site: Y71 (= Y210), S72 (= S211), C250 (= C380), E290 (= E420), Y292 (= Y422)
- binding flavin-adenine dinucleotide: R69 (= R208), L70 (≠ Q209), Y71 (= Y210), S72 (= S211), C90 (≠ T229), Y96 (≠ T235), G107 (= G244), V108 (≠ I245), C109 (≠ A246), S110 (= S247), T150 (= T286), E290 (= E420), Y292 (= Y422)
P00455 Ferredoxin--NADP reductase, chloroplastic; FNR; EC 1.18.1.2 from Spinacia oleracea (Spinach) (see 3 papers)
34% identity, 66% coverage: 144:422/422 of query aligns to 85:369/369 of P00455
- RLYS 148:151 (≠ RQYS 208:211) binding
- CVK 169:171 (≠ TVK 229:231) binding
- Y175 (≠ T235) binding
- VCS 186:188 (≠ IAS 245:247) binding
- T227 (= T286) binding
- E367 (= E420) mutation to A: Slightly reduced activity.; mutation E->D,Q: Reduced activity.; mutation to L: Reduces activity by 99%.
Sites not aligning to the query:
- 1:55 modified: transit peptide, Chloroplast
P10933 Ferredoxin--NADP reductase, leaf isozyme, chloroplastic; FNR; EC 1.18.1.2 from Pisum sativum (Garden pea) (Lathyrus oleraceus) (see 2 papers)
35% identity, 66% coverage: 143:422/422 of query aligns to 75:360/360 of P10933
- RLYS 139:142 (≠ RQYS 208:211) binding
- S142 (= S211) binding
- CVK 160:162 (≠ TVK 229:231) binding
- K162 (= K231) binding
- Y166 (≠ T235) binding
- VCS 177:179 (≠ IAS 245:247) binding
- T218 (= T286) binding
- VP 250:251 (≠ AR 318:319) binding
- SR 280:281 (= SR 345:346) binding
- K290 (= K352) binding
- GL 319:320 (= GL 381:382) binding
- E358 (= E420) binding
- Y360 (= Y422) mutation to F: Results in a 2.0-fold reduction in kcat for the diaphorase reaction.; mutation to G: Results in a 302-fold reduction in kcat for the diaphorase reaction.; mutation to S: Results in a 22-fold reduction in kcat for the diaphorase reaction.; mutation to W: Results in a 2.2-fold reduction in kcat for the diaphorase reaction.
3mhpA Fnr-recruitment to the thylakoid (see paper)
35% identity, 66% coverage: 143:422/422 of query aligns to 10:295/296 of 3mhpA
- active site: Y76 (= Y210), S77 (= S211), C253 (= C380), E293 (= E420), Y295 (= Y422)
- binding flavin-adenine dinucleotide: R74 (= R208), L75 (≠ Q209), Y76 (= Y210), S77 (= S211), C95 (≠ T229), L99 (≠ V233), G111 (= G244), V112 (≠ I245), C113 (≠ A246), S114 (= S247), T153 (= T286), E293 (= E420), Y295 (= Y422)
- binding : L25 (≠ G158), T27 (≠ F160), K28 (≠ N161), I29 (≠ C162), H40 (= H173), F86 (≠ R220), G87 (≠ P221), W163 (= W297), F167 (≠ L301), E168 (≠ R302), K169 (≠ S303), E171 (≠ G305), S188 (≠ Q321), S189 (≠ Q322), E195 (≠ G328), E196 (≠ P329), K199 (≠ N332), K203 (vs. gap)
1qgaA Pea fnr y308w mutant in complex with NADP+ (see paper)
34% identity, 66% coverage: 143:422/422 of query aligns to 23:308/308 of 1qgaA
- active site: Y89 (= Y210), S90 (= S211), C266 (= C380), E306 (= E420), W308 (≠ Y422)
- binding flavin-adenine dinucleotide: R87 (= R208), L88 (≠ Q209), Y89 (= Y210), S90 (= S211), C108 (≠ T229), L112 (≠ V233), Y114 (≠ T235), G124 (= G244), V125 (≠ I245), C126 (≠ A246), S127 (= S247), T166 (= T286), W308 (≠ Y422)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: S90 (= S211), K110 (= K231), G165 (= G285), T166 (= T286), G167 (= G287), V198 (≠ A318), P199 (≠ R319), S228 (= S345), R229 (= R346), K238 (= K352), Y240 (= Y354), Q242 (= Q356), C266 (= C380), G267 (= G381), L268 (= L382), E306 (= E420), W308 (≠ Y422)
1fncA Refined crystal structure of spinach ferredoxin reductase at 1.7 angstroms resolution: oxidized, reduced, and 2'-phospho-5'-amp bound states (see paper)
34% identity, 66% coverage: 143:422/422 of query aligns to 11:296/296 of 1fncA
- active site: Y77 (= Y210), S78 (= S211), C254 (= C380), E294 (= E420), Y296 (= Y422)
- binding adenosine-2'-5'-diphosphate: K98 (= K231), V186 (≠ A318), P187 (≠ R319), S216 (= S345), R217 (= R346), K226 (= K352), Y228 (= Y354), L256 (= L382), G258 (≠ S384)
- binding dihydroflavine-adenine dinucleotide: R75 (= R208), L76 (≠ Q209), Y77 (= Y210), S78 (= S211), C96 (≠ T229), L100 (≠ V233), Y102 (≠ T235), G112 (= G244), V113 (≠ I245), C114 (≠ A246), S115 (= S247), T154 (= T286), E294 (= E420), Y296 (= Y422)
1bx0A Ferredoxin:nadp+ oxidoreductase (ferredoxin reductase) mutant e312l (see paper)
34% identity, 66% coverage: 143:422/422 of query aligns to 11:296/296 of 1bx0A
- active site: Y77 (= Y210), S78 (= S211), C254 (= C380), L294 (≠ E420), Y296 (= Y422)
- binding flavin-adenine dinucleotide: R75 (= R208), L76 (≠ Q209), Y77 (= Y210), S78 (= S211), C96 (≠ T229), Y102 (≠ T235), G112 (= G244), V113 (≠ I245), C114 (≠ A246), S115 (= S247), T154 (= T286), Y296 (= Y422)
1qfzA Pea fnr y308s mutant in complex with NADPH (see paper)
35% identity, 66% coverage: 143:421/422 of query aligns to 23:307/308 of 1qfzA
- active site: Y89 (= Y210), S90 (= S211), C266 (= C380), E306 (= E420)
- binding flavin-adenine dinucleotide: R87 (= R208), L88 (≠ Q209), Y89 (= Y210), S90 (= S211), C108 (≠ T229), K110 (= K231), L112 (≠ V233), Y114 (≠ T235), G124 (= G244), V125 (≠ I245), C126 (≠ A246), S127 (= S247), T166 (= T286)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: S90 (= S211), K110 (= K231), G165 (= G285), T166 (= T286), G167 (= G287), V198 (≠ A318), P199 (≠ R319), S228 (= S345), R229 (= R346), Y240 (= Y354), C266 (= C380), G267 (= G381), L268 (= L382), G270 (≠ S384), E306 (= E420)
Sites not aligning to the query:
1qfyA Pea fnr y308s mutant in complex with NADP+ (see paper)
35% identity, 66% coverage: 143:421/422 of query aligns to 23:307/308 of 1qfyA
- active site: Y89 (= Y210), S90 (= S211), C266 (= C380), E306 (= E420)
- binding flavin-adenine dinucleotide: R87 (= R208), L88 (≠ Q209), Y89 (= Y210), S90 (= S211), C108 (≠ T229), L112 (≠ V233), Y114 (≠ T235), G124 (= G244), V125 (≠ I245), C126 (≠ A246), S127 (= S247), T166 (= T286)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: S90 (= S211), K110 (= K231), G165 (= G285), T166 (= T286), G167 (= G287), V198 (≠ A318), P199 (≠ R319), S228 (= S345), R229 (= R346), K238 (= K352), Y240 (= Y354), Q242 (= Q356), C266 (= C380), G267 (= G381), L268 (= L382), G270 (≠ S384), M271 (= M385), E306 (= E420)
Sites not aligning to the query:
2vzlA Ferredoxin-NADP reductase (mutations: t155g, a160t, l263p and y303s) complexed with NAD by cocrystallization (see paper)
32% identity, 66% coverage: 143:421/422 of query aligns to 4:294/295 of 2vzlA
- active site: Y71 (= Y210), S72 (= S211), C253 (= C380), E293 (= E420)
- binding flavin-adenine dinucleotide: R69 (= R208), L70 (≠ Q209), Y71 (= Y210), S72 (= S211), C90 (≠ T229), L94 (≠ V233), Y96 (≠ T235), G107 (= G244), V108 (≠ I245), C109 (≠ A246), S110 (= S247), T149 (= T286), E293 (= E420)
- binding nicotinamide-adenine-dinucleotide: S72 (= S211), R92 (≠ K231), T149 (= T286), C253 (= C380), G254 (= G381), P255 (≠ L382), E293 (= E420)
Sites not aligning to the query:
5h59A Ferredoxin-NADP+ reductase from maize root (see paper)
34% identity, 68% coverage: 137:422/422 of query aligns to 14:311/311 of 5h59A
- active site: Y88 (= Y210), S89 (= S211), F268 (≠ V379), C269 (= C380), E309 (= E420), Y311 (= Y422)
- binding flavin-adenine dinucleotide: R86 (= R208), L87 (≠ Q209), Y88 (= Y210), S89 (= S211), C107 (≠ T229), Y113 (vs. gap), K125 (≠ V242), G127 (= G244), V128 (≠ I245), C129 (≠ A246), S130 (= S247), T170 (= T286), Y311 (= Y422)
5vw4A Nicotinamide soak of y316s mutant of corn root ferredoxin:nadp+ reductase (see paper)
35% identity, 68% coverage: 137:421/422 of query aligns to 12:308/309 of 5vw4A
- active site: Y86 (= Y210), S87 (= S211), F266 (≠ V379), C267 (= C380), E307 (= E420)
- binding flavin-adenine dinucleotide: R84 (= R208), L85 (≠ Q209), Y86 (= Y210), S87 (= S211), C105 (≠ T229), A109 (≠ V233), Y111 (vs. gap), K123 (≠ V242), G125 (= G244), V126 (≠ I245), C127 (≠ A246), S128 (= S247), T168 (= T286)
- binding nicotinamide: S87 (= S211), T168 (= T286), G169 (= G287), C267 (= C380), G268 (= G381), E307 (= E420), V308 (≠ T421)
Sites not aligning to the query:
5vw2A NADPH soak of y316s mutant of corn root ferredoxin:nadp+ reductase (see paper)
35% identity, 68% coverage: 137:421/422 of query aligns to 12:308/309 of 5vw2A
- active site: Y86 (= Y210), S87 (= S211), F266 (≠ V379), C267 (= C380), E307 (= E420)
- binding dihydroflavine-adenine dinucleotide: R84 (= R208), L85 (≠ Q209), Y86 (= Y210), S87 (= S211), C105 (≠ T229), A109 (≠ V233), Y111 (vs. gap), K123 (≠ V242), G125 (= G244), V126 (≠ I245), C127 (≠ A246), S128 (= S247), T168 (= T286)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: S87 (= S211), R107 (≠ K231), T166 (= T284), G167 (= G285), T168 (= T286), G169 (= G287), G199 (= G317), V200 (≠ A318), A201 (≠ R319), S230 (= S345), R231 (= R346), K240 (= K352), Y242 (= Y354), Q244 (= Q356), G268 (= G381), L269 (= L382), G271 (≠ S384), M272 (= M385), E307 (= E420), V308 (≠ T421)
Sites not aligning to the query:
P53991 Ferredoxin--NADP reductase, chloroplastic; FNR; EC 1.18.1.2 from Chlamydomonas reinhardtii (Chlamydomonas smithii)
34% identity, 66% coverage: 144:422/422 of query aligns to 64:354/354 of P53991
- K118 (vs. gap) modified: N6,N6,N6-trimethyllysine
- K124 (= K203) modified: N6,N6,N6-trimethyllysine
- K170 (≠ R243) modified: N6,N6-dimethyllysine
Sites not aligning to the query:
- 1:35 modified: transit peptide, Chloroplast
2rc6A Refined structure of fnr from leptospira interrogans bound to NADP+ (see paper)
33% identity, 67% coverage: 141:422/422 of query aligns to 5:306/306 of 2rc6A
- active site: Y90 (= Y210), S91 (= S211), C264 (= C380), E304 (= E420), Y306 (= Y422)
- binding flavin-adenine dinucleotide: R88 (= R208), L89 (≠ Q209), Y90 (= Y210), S91 (= S211), I109 (≠ T229), I110 (≠ V230), D113 (≠ V233), I115 (≠ T235), F122 (≠ V242), G124 (= G244), V125 (≠ I245), C126 (≠ A246), S127 (= S247), T167 (= T286), E304 (= E420), Y306 (= Y422)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: K111 (= K231), T165 (= T284), G166 (= G285), A197 (= A318), P198 (≠ R319), S226 (= S345), R227 (= R346), R237 (≠ K352), Y239 (= Y354), P267 (vs. gap), M270 (= M385)
- binding zinc ion: E181 (≠ R300), H216 (≠ P334)
Query Sequence
>Ac3H11_3083 FitnessBrowser__acidovorax_3H11:Ac3H11_3083
MDMAVEAAVIKQHLIDPEICIRCNTCEATCPVGAITHDDNNYVVRADVCNGCMACISPCP
TGSIDNWRTMPLARAYTIEEQLTWEELPPELPPDALAAEGVAPSADAVPPTTPAAPVEPG
TQAFRSAQYGATVPPWSAAHPYTNLFPPKSPTEATVVGNFNCTEAGFENETHHVVLDFGS
MPFPVLEGQSIGIIPPGTDALGKEHHARQYSIASPRNGERPGYNNLALTVKRVTTDHDGN
AVRGIASNFVCDLKVGDKVQVVGPFGSSFLMPNHPRSHIVMICTGTGSAPMRAMTEWRRR
LRSSGKFEGGKLMLFFGARTQQELPYFGPLQNLPKDFIDINLAFSRTAGQPKRYVQDLMR
ERAADLAALLRDGSGHFYVCGLKSMEEGVVMALRDIASEAGLDWDTVGAALQREGRLHLE
TY
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory