SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ac3H11_3085 FitnessBrowser__acidovorax_3H11:Ac3H11_3085 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 5 papers)
33% identity, 31% coverage: 51:222/556 of query aligns to 60:224/763 of P40939

query
sites
P40939

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Sites not aligning to the query:

282 V → D: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852773
305 I → N: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852774
342 L → P: in LCHAD deficiency; dbSNP:rs137852772
510 active site, For hydroxyacyl-coenzyme A dehydrogenase activity; E → Q: in AFLP and LCHAD deficiency; loss of long-chain-3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137852769

5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
32% identity, 34% coverage: 51:241/556 of query aligns to 24:203/259 of 5zaiC

query
sites
5zaiC

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active site: A65 (= A94), F70 (= F97), S82 (≠ C111), R86 (≠ N115), G110 (= G143), E113 (= E146), P132 (= P167), E133 (= E168), I138 (≠ V174), P140 (= P176), G141 (= G177)
binding coenzyme a: K24 (= K51), L25 (= L52), A63 (≠ S92), G64 (= G93), A65 (= A94), D66 (≠ N95), I67 (= I96), P132 (= P167), R166 (≠ G204)

Sites not aligning to the query:

active site: 226, 236
binding coenzyme a: 248, 251

6yswA E. Coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme a
33% identity, 30% coverage: 51:219/556 of query aligns to 24:185/707 of 6yswA

query
sites
6yswA

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active site: A66 (= A94), I71 (≠ L99), A84 (vs. gap), Q88 (≠ I121), G112 (= G143), E115 (= E146), P136 (= P167), E137 (= E168), G145 (= G177)
binding coenzyme a: M25 (≠ L52), A66 (= A94), D67 (≠ N95), I68 (= I96), P136 (= P167), E137 (= E168), L140 (= L172)

Sites not aligning to the query:

active site: 264, 422, 443, 455, 493
binding coenzyme a: 23, 290, 293

5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
31% identity, 32% coverage: 62:241/556 of query aligns to 36:205/261 of 5jbxB

query
sites
5jbxB

E

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active site: A67 (= A94), R72 (≠ L99), L84 (≠ C111), R88 (≠ N115), G112 (= G143), E115 (= E146), T134 (≠ P167), E135 (= E168), I140 (≠ V174), P142 (= P176), G143 (= G177)
binding coenzyme a: A65 (≠ S92), D68 (≠ N95), L69 (≠ I96), K70 (≠ F97), L110 (≠ A141), G111 (= G142), T134 (≠ P167), E135 (= E168), L138 (= L172), R168 (≠ G204)

Sites not aligning to the query:

active site: 228, 238
binding coenzyme a: 24, 25, 26, 28

2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
32% identity, 30% coverage: 52:220/556 of query aligns to 28:183/260 of 2hw5C

query
sites
2hw5C

L
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active site: A68 (= A94), M73 (= M98), S83 (≠ K107), L87 (≠ C111), G111 (= G143), E114 (= E146), P133 (= P167), E134 (= E168), T139 (≠ V174), P141 (= P176), G142 (= G177)
binding crotonyl coenzyme a: L28 (= L52), A30 (≠ S54), K62 (≠ R88), I70 (= I96), F109 (≠ A141)

Sites not aligning to the query:

active site: 227, 237
binding crotonyl coenzyme a: 26, 27

2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
30% identity, 30% coverage: 52:219/556 of query aligns to 27:176/254 of 2dubA

query
sites
2dubA

L
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L

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active site: A67 (= A94), M72 (≠ L99), S82 (≠ C111), G105 (= G143), E108 (= E146), P127 (= P167), E128 (= E168), T133 (≠ V174), P135 (= P176), G136 (= G177)
binding octanoyl-coenzyme a: L27 (= L52), A29 (≠ S54), A65 (≠ S92), A67 (= A94), D68 (≠ N95), I69 (= I96), K70 (≠ F97), G105 (= G143), E108 (= E146), P127 (= P167), E128 (= E168), G136 (= G177), A137 (≠ T178)

Sites not aligning to the query:

active site: 221, 231
binding octanoyl-coenzyme a: 25, 26

1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
30% identity, 30% coverage: 52:219/556 of query aligns to 28:180/258 of 1mj3A

query
sites
1mj3A

L
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L

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L

D

C

L

N

G

G

V

L

D

I

I

E

E

E

L

L

H

N

D

Q

A

A

L

L

N

E

R

T

I

F

R

E

F

-

E

E

H

D

P

P

Q

A

V

V

R

G

S

A

V

I

I

V

V

L

T

T

S

G

A

G

R

-

D

E

R

K

I

A

F

F

C

A

S
x
A

G
|
G

A
|
A

N
x
D

I
|
I

F

-

M

-

L

-

G

-

V

-

S

-

H

-

A

-

W

-

K

-

V

-

N

-

F

-

C

-

K

-

F

-

T

-

N

K

E

E

T
x
M

R

Q

N

N

-

R

G

T

I

F

E

Q

D

D

S

C

S

-

K

-

H

Y

S
|
S

G

G

L
|
L

K

S

-

H

-

W

-

D

-

H

-

I

-

T

-

R

-

I

-

K

-

P

F

V

V

I

A

A

V

V

N

N

G

G

A

Y

C

A

A

L

G

G

G
|
G

G

G

Y

C

E
|
E

L

L

A

A

L

M

A

M

C

C

D

D

E

-

I

-

V

I

L

I

V

Y

D

A

D

G

R

E

S

K

S

A

A

Q

V

F

S

G

L

Q

P
|
P

E
|
E

V

I

P

-

L

L

L
|
L

G

G

V
x
T

L

I

P
|
P

G
|
G

T

A

G

G

L

T

T

Q

R

R

V

L

T

T

D

-

K

-

R

R

H

A

V

V

R

G

H

K

D

S

L

L

A

A

D

M

I

E

F

M

C

V

T

L

S

T

V

G

E

D

G

R

V

I

R

S

G

A

Q

Q

R

D

A

A

V

K

D

Q

W

A

R

G

L

L

V

V

D

S

R

K

I

I

active site: A68 (= A94), M73 (≠ T117), S83 (= S128), L85 (= L130), G109 (= G143), E112 (= E146), P131 (= P167), E132 (= E168), T137 (≠ V174), P139 (= P176), G140 (= G177)
binding hexanoyl-coenzyme a: L28 (= L52), A30 (≠ S54), A66 (≠ S92), G67 (= G93), A68 (= A94), D69 (≠ N95), I70 (= I96), G109 (= G143), P131 (= P167), E132 (= E168), L135 (= L172), G140 (= G177)

Sites not aligning to the query:

active site: 225, 235
binding hexanoyl-coenzyme a: 26, 27

Q4WF54 Mevalonyl-coenzyme A hydratase sidH; Siderophore biosynthesis protein H; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
29% identity, 30% coverage: 74:241/556 of query aligns to 53:210/270 of Q4WF54

query
sites
Q4WF54

H

Q

P

P

Q

A

V

L

R

Y

S

V

V

A

I

I

V

I

T

T

S

G

A

T

R

G

D

E

R

S

I

-

F

F

C

C

S

A

G

G

A

A

N

D

I

L

F

-

M

-

L

-

G

-

V

-

S

-

H

K

A

E

W

W

K

N

-

D

V

L

N

N

F

A

C

R

K

G

F

I

T

T

N

N

E

E

-

M

T

T

R

A

N

P

G

G

I

L

E

A

D

G

S

L

S

P

K

R

H

R

S

R

G

G

L

S

K

K

-

P

F

I

V

I

A

A

V

V

N

N

G

G

A

Y

C

C

A

L

G

G

Y

F

E

E

L

M

A

V

L

A

A

N

C

C

D

D

E

-

I

-

V

I

L

V

V

V

D

A

D

S

R

E

S

N

S

A

A

T

V

F

S

G

L

L

P

P

E

E

V

V

P

Q

L

R

L

-

G

G

V

I

L

A

P

A

G

V

T

A

G

G

G

S

L

L

T

P

R

R

V

L

T

V

D

-

K

-

R

R

H

V

V

L

R

G

H

K

D

Q

L

R

A

A

D

A

I

E

F

I

C

A

T

L

S

S

V

G

E

L

G

S

V

F

R

S

G

A

Q

S

R

Q

A

L

V

E

D

R

W

W

R

G

L

L

V

V

D

N

R

R

I

V

A

V

K

E

P

H

A

D

Q

Q

F

L

A

L

A

A

A

T

V

A

D

V

D

E

T

I

A

A

A

T

R

A

L

I

T

S

E

R

G

N

S

S

P

P

Sites not aligning to the query:

268:270 PTS1-type peroxisomal targeting signal

P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
30% identity, 30% coverage: 52:219/556 of query aligns to 58:212/290 of P14604

query
sites
P14604

L

L

N

N

S

A

Y

L

D

C

L

N

G

G

V

L

D

I

I

E

E

E

L

L

H

N

D

Q

A

A

L

L

N

E

R

T

I

F

R

E

F

-

E

E

H

D

P

P

Q

A

V

V

R

G

S

A

V

I

I

V

V

L

T

T

S

G

A

G

R

-

D

E

R

K

I

A

F

F

C

A

S

A

G

G

A

A

N

D

I

I

F

K

M

E

L

M

G

-

V

-

S

Q

S

N

H

R

A

T

W

F

K

Q

V

D

N

C

F

Y

C

S

-

G

K

K

F

F

T

L

N

S

E

H

T

W

R

-

N

-

G

-

I

-

E

-

D

D

S

H

S

I

K

T

H

R

S

I

G

K

L

K

K

P

F

V

V

I

A

A

V

V

N

N

G

G

A

Y

C

A

A

L

G

G

Y

C

E
|
E

L

L

A

A

L

M

A

M

C

C

D

D

E

-

I

-

V

I

L

I

V

Y

D

A

D

G

R

E

S

K

S

A

A

Q

V

F

S

G

L

Q

P

P

E
|
E

V

I

P

-

L

L

G

G

V

T

L

I

P

P

G

G

T

A

G

G

L

T

T

Q

R

R

V

L

T

T

D

-

K

-

R

R

H

A

V

V

R

G

H

K

D

S

L

L

A

A

D

M

I

E

F

M

C

V

T

L

S

T

V

G

E

D

G

R

V

I

R

S

G

A

Q

Q

R

D

A

A

V

K

D

Q

W

A

R

G

L

L

V

V

D

S

R

K

I

I

E144 (= E146) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
E164 (= E168) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.

Sites not aligning to the query:

1:29 modified: transit peptide, Mitochondrion

1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
30% identity, 30% coverage: 52:219/556 of query aligns to 28:182/260 of 1dubA

query
sites
1dubA

L
|
L

N

N

S
x
A

Y

L

D

C

L

N

G

G

V

L

D

I

I

E

E

E

L

L

H

N

D

Q

A

A

L

L

N

E

R

T

I

F

R

E

F

-

E

E

H

D

P

P

Q

A

V

V

R

G

S

A

V

I

I

V

V

L

T

T

S

G

A

G

R

-

D

E

R

K

I

A

F

F

C

A

S
x
A

G

G

A
|
A

N
x
D

I
|
I

F

K

M

E

L
x
M

G

-

V

-

S

Q

S

N

H

R

A

T

W

F

K

Q

V

D

N

C

F

Y

C
x
S

-

G

K

K

F

F

T
x
L

N

S

E

H

T

W

R

-

N

-

G

-

I

-

E

-

D

D

S

H

S

I

K

T

H

R

S

I

G

K

L

K

K

P

F

V

V

I

A

A

V

V

N

N

G

G

A
x
Y

C

A

A

L

G
|
G

G

G

Y

C

E
|
E

L

L

A

A

L

M

A

M

C

C

D

D

E

-

I

-

V

I

L

I

V

Y

D

A

D

G

R

E

S

K

S

A

A

Q

V

F

S

G

L

Q

P
|
P

E
|
E

V

I

P

-

L

L

L
|
L

G

G

V
x
T

L

I

P
|
P

G
|
G

T

A

G

G

L

T

T

Q

R

R

V

L

T

T

D

-

K

-

R

R

H

A

V

V

R

G

H

K

D

S

L

L

A

A

D

M

I

E

F

M

C

V

T

L

S

T

V

G

E

D

G

R

V

I

R

S

G

A

Q

Q

R

D

A

A

V

K

D

Q

W

A

R

G

L

L

V

V

D

S

R

K

I

I

active site: A68 (= A94), M73 (≠ L99), S83 (≠ C111), L87 (≠ T114), G111 (= G143), E114 (= E146), P133 (= P167), E134 (= E168), T139 (≠ V174), P141 (= P176), G142 (= G177)
binding acetoacetyl-coenzyme a: L28 (= L52), A30 (≠ S54), A66 (≠ S92), A68 (= A94), D69 (≠ N95), I70 (= I96), Y107 (≠ A139), G110 (= G142), G111 (= G143), E114 (= E146), P133 (= P167), E134 (= E168), L137 (= L172), G142 (= G177)

Sites not aligning to the query:

active site: 227, 237
binding acetoacetyl-coenzyme a: 26, 27, 233, 249

1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
30% identity, 30% coverage: 52:219/556 of query aligns to 26:180/258 of 1ey3A

query
sites
1ey3A

L
|
L

N

N

S
x
A

Y

L

D

C

L

N

G

G

V

L

D

I

I

E

E

E

L

L

H

N

D

Q

A

A

L

L

N

E

R

T

I

F

R

E

F

-

E

E

H

D

P

P

Q

A

V

V

R

G

S

A

V

I

I

V

V

L

T

T

S

G

A

G

R

-

D

E

R

K

I

A

F

F

C

A

S
x
A

G
|
G

A
|
A

N
x
D

I
|
I

F

K

M

E

L
x
M

G

-

V

-

S

Q

S

N

H

R

A

T

W

F

K

Q

V

D

N

C

F

Y

C
x
S

-

G

K

K

F

F

T
x
L

N

S

E

H

T
x
W

R

-

N

-

G

-

I

-

E

-

D

D

S

H

S

I

K

T

H

R

S

I

G

K

L

K

K

P

F

V

V

I

A

A

V

V

N

N

G

G

A

Y

C

A

A

L

G

G

G
|
G

G

G

Y

C

E
|
E

L

L

A

A

L

M

A

M

C

C

D

D

E

-

I

-

V

I

L

I

V

Y

D

A

D

G

R

E

S

K

S

A

A

Q

V

F

S

G

L

Q

P
|
P

E
|
E

V

I

P

-

L

L

L
|
L

G

G

V
x
T

L

I

P
|
P

G
|
G

T

A

G

G

L

T

T

Q

R

R

V

L

T

T

D

-

K

-

R

R

H

A

V

V

R

G

H

K

D

S

L

L

A

A

D

M

I

E

F

M

C

V

T

L

S

T

V

G

E

D

G

R

V

I

R

S

G

A

Q

Q

R

D

A

A

V

K

D

Q

W

A

R

G

L

L

V

V

D

S

R

K

I

I

active site: A66 (= A94), M71 (≠ L99), S81 (≠ C111), L85 (≠ T114), G109 (= G143), E112 (= E146), P131 (= P167), E132 (= E168), T137 (≠ V174), P139 (= P176), G140 (= G177)
binding 4-(n,n-dimethylamino)cinnamoyl-coa: L26 (= L52), A28 (≠ S54), A64 (≠ S92), G65 (= G93), A66 (= A94), D67 (≠ N95), I68 (= I96), L85 (≠ T114), W88 (≠ T117), G109 (= G143), P131 (= P167), L135 (= L172), G140 (= G177)

Sites not aligning to the query:

active site: 225, 235
binding 4-(n,n-dimethylamino)cinnamoyl-coa: 24

8pf8A Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-72
33% identity, 19% coverage: 63:169/556 of query aligns to 47:151/729 of 8pf8A

query
sites
8pf8A

L

M

H

G

D

K

A

A

L

V

N

D

R

R

I

L

R

V

F

A

E

E

H

K

P

D

Q

S

V

I

R

T

S

G

V

V

I

V

V

V

T

A

S

S

A

A

R

K

D

K

R

T

I

F

F

F

C

A

S
x
G

G
|
G

A
x
D

N

V

I

K

F
x
T

M
|
M

L

I

G

Q

V

A

S

R

S

P

H

E

A

D

W

A

K

G

V
x
D

N
x
V

F

F

C

N

K

-

F

-

T

T

N

V

E

E

T

T

R

I

N

K

G

R

I

Q

E

L

D

R

S

T

S

L

K

E

H

T

S

L

G

G

L

K

K

P

F

V

V

V

A

A

V

I

N

N

G

G

A

A

C

A

A

L

G

G

Y

L

E

E

L

I

A

A

L

L

A

A

C

C

D

H

E

H

I

R

V

I

L

A

V

A

D

D

D

V

R

K

S

G

S

S

A

Q

V

L

S

G

L

L

P

P

E

E

V

V

binding [2-methyl-5-(trifluoromethyl)pyrazol-3-yl]boronic acid: G76 (≠ S92)
binding bis[2-methyl-5-(trifluoromethyl)pyrazol-3-yl]-bis(oxidanyl)boranuide: T81 (≠ F97), D92 (≠ V108), V93 (≠ N109)
binding [(2~{R})-2,3-bis(oxidanyl)propoxy]-[2-methyl-5-(trifluoromethyl)pyrazol-3-yl]borinic acid: G77 (= G93), D78 (≠ A94), M82 (= M98), V93 (≠ N109)

Sites not aligning to the query:

8oquA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-92
33% identity, 19% coverage: 63:169/556 of query aligns to 48:152/730 of 8oquA

query
sites
8oquA

L

M

H

G

D

K

A

A

L

V

N

D

R

R

I

L

R

V

F

A

E

E

H

K

P

D

Q

S

V

I

R

T

S

G

V

V

I

V

V

V

T

A

S

S

A

A

R

K

D

K

R

T

I

F

F

F

C

A

S
x
G

G
|
G

A
x
D

N
x
V

I

K

F

T

M

M

L

I

G

Q

V

A

S

R

S

P

H

E

A
x
D

W

A

K

G

V

D

N
x
V

F

F

C

N

K

-

F

-

T

T

N

V

E

E

T

T

R

I

N

K

G

R

I

Q

E

L

D

R

S

T

S

L

K

E

H

T

S

L

G

G

L

K

K

P

F

V

V

V

A

A

V

I

N

N

G

G

A

A

C

A

A
x
L

G
|
G

G

G

Y

L

E

E

L

I

A

A

L

L

A

A

C

C

D

H

E

H

I

R

V

I

L

A

V

A

D

D

D

V

R

K

S

G

S

S

A

Q

V

L

S

G

L

L

P
|
P

E
|
E

V

V

binding 4-chloranylbenzenesulfonic acid: G77 (≠ S92), G78 (= G93), D79 (≠ A94), V80 (≠ N95), D90 (≠ A105), V94 (≠ N109), L124 (≠ A141), G125 (= G142), P150 (= P167), E151 (= E168)

Sites not aligning to the query:

binding 4-chloranylbenzenesulfonic acid: 40, 41, 42

8oqtA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-91
33% identity, 19% coverage: 63:169/556 of query aligns to 47:151/729 of 8oqtA

query
sites
8oqtA

L

M

H

G

D

K

A

A

L

V

N

D

R

R

I

L

R

V

F

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x
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N

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binding 4-bromanylbenzenesulfonic acid: G76 (≠ S92), G77 (= G93), D78 (≠ A94), V79 (≠ N95), M82 (= M98), D89 (≠ A105), V93 (≠ N109), T96 (= T114), T96 (= T114), P149 (= P167), E150 (= E168)

Sites not aligning to the query:

binding 4-bromanylbenzenesulfonic acid: 5, 41

8oqnA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-53
33% identity, 19% coverage: 63:169/556 of query aligns to 47:151/729 of 8oqnA

query
sites
8oqnA

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binding 1-benzyl-1H-pyrazole-4-carboxylic acid: M82 (= M98), E150 (= E168)

Sites not aligning to the query:

binding 1-benzyl-1H-pyrazole-4-carboxylic acid: 39, 172, 175, 176, 181, 241, 254, 257, 261, 262, 266, 268, 282, 283, 302, 303, 304, 521, 525

8opvA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with resveratrol (fragment-b-h11)
33% identity, 19% coverage: 63:169/556 of query aligns to 47:151/729 of 8opvA

query
sites
8opvA

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binding resveratrol: A75 (≠ C91), G76 (≠ S92), M82 (= M98), E128 (= E146), P149 (= P167), E150 (= E168)

Sites not aligning to the query:

binding resveratrol: 39, 152, 153, 184, 296

8opuA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with sulfamethoxazole (fragment-b-e1)
33% identity, 19% coverage: 63:169/556 of query aligns to 47:151/729 of 8opuA

query
sites
8opuA

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Sites not aligning to the query:

binding Sulfamethoxazole: 450, 473, 521, 678, 679

8oqrA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-80
33% identity, 19% coverage: 63:169/556 of query aligns to 47:151/728 of 8oqrA

query
sites
8oqrA

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binding 4-cyanobenzenesulfonic acid: G76 (≠ S92), G77 (= G93), T81 (≠ F97), M82 (= M98), M82 (= M98), A85 (≠ V101), D89 (≠ A105), T96 (= T114), L123 (≠ A141), G124 (= G142), P149 (= P167), E150 (= E168)

Sites not aligning to the query:

binding 4-cyanobenzenesulfonic acid: 366, 367, 368, 420, 421, 422

4b3iA Crystal structure of mycobacterium tuberculosis fatty acid beta-oxidation complex with coenzymea bound at the hydratase active sites (see paper)
33% identity, 19% coverage: 63:169/556 of query aligns to 49:153/731 of 4b3iA

query
sites
4b3iA

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active site: G79 (= G93), E100 (= E116), R104 (≠ G120), G127 (= G143), E130 (= E146), P151 (= P167), E152 (= E168)
binding coenzyme a: A77 (≠ C91), G79 (= G93), D80 (≠ A94), V81 (≠ N95), E152 (= E168)

Sites not aligning to the query:

active site: 160, 452, 473, 485, 523
binding adenosine-5'-diphosphate: 640, 641, 642, 643, 644
binding coenzyme a: 38, 40, 315, 319

4omrA Crystal structure of tfu_1878, a putative enoyl-coa hydratase from thermobifida fusca yx in complex with acetoacetyl-coa
25% identity, 37% coverage: 25:230/556 of query aligns to 15:208/255 of 4omrA

query
sites
4omrA

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active site: I70 (≠ A94), F75 (≠ L99), I98 (= I121), Q102 (≠ S125), A126 (≠ G143), Q129 (≠ E146), K148 (≠ P167), E149 (= E168), D157 (≠ G177)
binding acetoacetyl-coenzyme a: H30 (≠ G47), R31 (≠ Y48), R32 (≠ K49), A34 (≠ K51), S68 (= S92), I70 (≠ A94), D71 (≠ N95), L72 (≠ I96), I124 (≠ A141), G125 (= G142), A126 (≠ G143), K148 (≠ P167), E149 (= E168)

Sites not aligning to the query:

active site: 242

Query Sequence

>Ac3H11_3085 FitnessBrowser__acidovorax_3H11:Ac3H11_3085
MTHPVQPPSRVDYRTDPTQYRHWKLAVDGAVARLSLDIAEDGGIRPGYKLKLNSYDLGVD
IELHDALNRIRFEHPQVRSVIVTSARDRIFCSGANIFMLGVSSHAWKVNFCKFTNETRNG
IEDSSKHSGLKFVAAVNGACAGGGYELALACDEIVLVDDRSSAVSLPEVPLLGVLPGTGG
LTRVTDKRHVRHDLADIFCTSVEGVRGQRAVDWRLVDRIAKPAQFAAAVDDTAARLTEGS
PRPSHAQGVALPRVEREETADSLRYTHVSVSIDRTRRTATLTLKAPTGPQPTDIADIEAA
GAAWWPLALARQLDDAILNLRTNELDIGTWLLKTEGDAQAVLASDATLIAHQGHWLVRET
IGALRRTFARLDVSSRSLFALVEPGSAFAGSLAELSFAADRTYMLALPDDTERAPKITLN
EFNFGLLPMVNDQSRLQRRFYEEAAPLEAARAAAGKPQDADAALALGLVTAAPDDIDWDD
EIRIAIEERAAMSPDSLTGLEANLRFASKENMNTRIFGRLTAWQNWIFNRPNAVGDKGAL
KVYGTGQKAGFDLNRV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory