SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ac3H11_3452 FitnessBrowser__acidovorax_3H11:Ac3H11_3452 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q63XL8 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Burkholderia pseudomallei (strain K96243) (see paper)
74% identity, 98% coverage: 3:319/324 of query aligns to 2:317/318 of Q63XL8

query
sites
Q63XL8

A

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RQ 99:100 (= RQ 100:101) binding

3dahC 2.3 a crystal structure of ribose-phosphate pyrophosphokinase from burkholderia pseudomallei (see paper)
73% identity, 95% coverage: 8:314/324 of query aligns to 2:300/300 of 3dahC

query
sites
3dahC

F

L

M

M

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V

F

F

T

T

G

G

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N

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binding adenosine monophosphate: F33 (= F39), D35 (= D41), E37 (= E43), R94 (= R100), Q95 (= Q101)

P0A717 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Escherichia coli (strain K12) (see 4 papers)
65% identity, 96% coverage: 6:317/324 of query aligns to 2:313/315 of P0A717

query
sites
P0A717

P

P

D

D

F

M

M

K

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L

F

F

T

A

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G

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A

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I

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H

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F

D129 (= D133) to A: in mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP
D220 (= D223) mutation to E: 4-fold decrease in the affinity binding for Rib-5-P in the presence of magnesium ions. In the presence of cobalt ions, it shows a 15-fold decrease in the affinity binding for Rib-5-P.; mutation to F: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.
D221 (= D224) mutation to A: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions.
D224 (= D227) mutation to A: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.; mutation to S: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6asvC E. Coli prpp synthetase (see paper)
65% identity, 96% coverage: 7:317/324 of query aligns to 1:311/311 of 6asvC

query
sites
6asvC

D

D

F

M

M

K

V

L

F

F

T

A

G

G

N

N

A

A

N

T

P

P

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E

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L

A

A

A

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I

A

A

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L

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D

A

A

D

A

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G

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R

F

F

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S

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D

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G

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E

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E

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I

I

K

N

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E

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G

D

D

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F

F

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S

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T

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C

A

A

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P

T

T

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N

E

D

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N

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M

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E

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D

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F

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D

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x
G

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L
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L

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K

K

A

A

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A

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K

E

E

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R

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G

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A

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K

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A

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H

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G

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F

binding phosphate ion: D168 (= D174), D218 (= D223), D219 (= D224), D222 (= D227), T223 (= T228), G224 (≠ A229), T226 (= T231), L227 (= L232)

4s2uA Crystal structure of the phosphorybosylpyrophosphate synthetase from e. Coli
66% identity, 95% coverage: 6:313/324 of query aligns to 1:308/308 of 4s2uA

query
sites
4s2uA

P

P

D

D

F

M

M

K

V

L

F

F

T

A

G

G

N

N

A

A

N

T

P

P

G

E

M

L

A

A

A

Q

E

R

I

I

A

A

Q

N

H

R

L

L

G

Y

T

T

T

S

L

L

G

G

A

D

A

A

D

A

V

V

G

G

R

R

F

F

S

S

D

D

G

G

E

E

V

V

T

S

V

V

E

Q

I

I

K

N

Q

E

N

N

V

V

R

R

A

G

R

G

D

D

V

I

F

F

V

I

Q

Q

S

S

T

T

C

C

A

A

P

P

T

T

N

N

E

D

N

N

L

L

M

M

E

E

L

L

L

V

I

V

M

M

V

V

D

D

A

A

L

L

K

R

R

R

A

A

S

S

A

A

E

G

R

R

I

I

S

T

A

A

V

V

I

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R

R

Q

Q

D

D

R

R

P

V

R

R

S

S

T

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

V

V

A

A

N

D

M

F

L

L

Q

S

A

S

V

V

G

G

V

V

A

D

R

R

V

V

L

L

T

T

M

V

D

D

L

L

H

H

A

A

D

E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

V

V

D

D

N

N

I

V

Y

F

A

G

S

S

P

P

V

I

L

L

G

E

D

D

L

M

R

L

Q

Q

K

L

N

N

Y

L

E

D

D

N

L

P

I

I

V

V

S

S

P

P

D

D

V

I

G

G

V

V

R

R

A

A

R

R

A

A

L

I

A

A

K

K

Q

L

L

L

G

N

-

D

C

T

D

D

L

M

A

A

I

I

D

D

K

K

R

R

P

P

K

R

A

A

N

N

V

V

S

S

E

Q

V

V

M

M

H

H

V

I

I

I

G

G

E

D

I

V

E

A

G

G

R

R

N

D

C

C

V

V

I

L

M

V

D

D

M

M

I

I

D
|
D

T

T

A

G

G

G

T
|
T

L

L

V

C

K

K

A

A

E

E

V

A

L

L

K

K

E

E

R

R

G

G

A

A

K

K

S

R

V

V

Y

F

A

A

Y

Y

C

A

T

T

H

H

P

P

I

I

F

F

S

S

G

G

P

N

A

A

I

A

E

N

R

N

I

L

A

-

K

R

G

N

S

S

A

V

L

I

D

D

E

E

V

V

T

C

N

D

T

T

I

I

P

P

L

L

S

S

D

D

N

E

A

I

K

K

G

S

C

L

T

P

K

N

I

V

R

R

Q

T

L

L

S

T

V

L

A

S

P

G

L

M

I

L

A

A

E

E

T

A

I

I

Q

R

R

R

I

I

A

S

K

N

G

E

E

E

S

S

V

I

binding magnesium ion: D223 (= D227), T227 (= T231)

7xmvA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a(amp/adp) filament bound with adp, amp and r5p (see paper)
63% identity, 96% coverage: 7:317/324 of query aligns to 1:305/307 of 7xmvA

query
sites
7xmvA

D

D

F

M

M

K

V

L

F

F

T

A

G

G

N

N

A

A

N

T

P

P

G

E

M

L

A

A

A

Q

E

R

I

I

A

A

Q

N

H

R

L

L

G

Y

T

T

T

S

L

L

G

G

A

D

A

A

D

A

V

V

G

G

R

R

F
|
F

S

S

D
|
D

G

G

E
|
E

V

V

T

S

V

V

E

Q

I

I

K

N

Q

E

N

N

V

V

R

R

A

G

R

G

D

D

V

I

F

F

V

I

Q

Q

S

S

T

T

C

C

A

A

P

P

T

T

N

N

E

D

N

N

L

L

M

M

E

E

L

L

L

V

I

V

M

M

V

V

D

D

A

A

L

L

K

R

R

R

A

A

S

S

A

A

E

G

R

R

I

I

S

T

A

A

V

V

I

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q

Q

D

D

R
|
R

R

R

P
x
V

R
|
R

S

S

T

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

V

V

A

A

N

D

M

F

L

L

Q

S

A

S

V

V

G

G

V

V

A

D

R

R

V

V

L

L

T

T

M

V

D

D

L

L

H
|
H

A

A

D
x
E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

V

V

D

D

N

N

I

V

Y
x
F

A

G

S
|
S

P

P

V

I

L

L

G

E

D

D

L

M

R

L

Q

Q

K

L

N

N

Y

L

E

D

D

N

L

P

I

I

V

V

S

S

P

P

D

D

V

I

G

G

V

V

V
|
V

R

R

A

A

R

R

A
|
A

L

I

A

A

K

K

Q

L

L

L

G

N

-

D

C

T

D

D

L

M

A

A

I

I

D

D

K

K

R

R

P

-

K

-

A

-

N

-

V

-

S

-

E

Q

V

V

M

M

H

H

V

I

I

I

G

G

E

D

I

V

E

A

G

G

R

R

N

D

C

C

V

V

I

L

M

V

D
|
D

M
|
M

I

I

D
|
D

T
|
T

A

G

G
|
G

T
|
T

L

L

V

C

K

K

A

A

E

E

V

A

L

L

K

K

E

E

R

R

G

G

A

A

K

K

S

R

V

V

Y

F

A

A

Y

Y

C

A

T

T

H

H

P

P

I

I

F

F

S

S

G

G

P

N

A

A

I

A

E

N

R

N

I

L

A

-

K

R

G

N

S

S

A

V

L

I

D

D

E

E

V

V

T

C

N

D

T

T

I

I

P

P

L

L

S

S

D

D

N

E

A

I

K

K

G

S

C

L

T

P

K

N

I

V

R

R

Q

T

L

L

S

T

V

L

A

S

P

G

L

M

I

L

A

A

E

E

T

A

I

I

Q

R

R

R

I

I

A

S

K

N

G

E

E

E

S

S

V

I

M

S

S

A

L

M

F

F

binding adenosine-5'-diphosphate: F33 (= F39), D35 (= D41), E37 (= E43), R94 (= R100), R97 (= R103), H129 (= H135)
binding adenosine monophosphate: R97 (= R103), V99 (≠ P105), R100 (= R106), E131 (≠ D137), F145 (≠ Y151), S147 (= S153), V173 (= V179), A177 (= A183)
binding 5-O-phosphono-alpha-D-ribofuranose: D212 (= D223), D213 (= D224), M214 (= M225), D216 (= D227), T217 (= T228), G219 (= G230), T220 (= T231)

7xmuA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a filament bound with adp, pi and r5p (see paper)
63% identity, 96% coverage: 7:317/324 of query aligns to 1:305/307 of 7xmuA

query
sites
7xmuA

D

D

F

M

M

K

V

L

F

F

T

A

G

G

N

N

A

A

N

T

P

P

G

E

M

L

A

A

A

Q

E

R

I

I

A

A

Q

N

H

R

L

L

G

Y

T

T

T

S

L

L

G

G

A

D

A

A

D

A

V

V

G

G

R

R

F
|
F

S

S

D
|
D

G

G

E
|
E

V

V

T

S

V

V

E

Q

I

I

K

N

Q

E

N

N

V

V

R

R

A

G

R

G

D

D

V

I

F

F

V

I

Q

Q

S

S

T

T

C

C

A

A

P

P

T

T

N

N

E

D

N

N

L

L

M

M

E

E

L

L

L

V

I

V

M

M

V

V

D

D

A

A

L

L

K

R

R

R

A

A

S

S

A

A

E

G

R

R

I

I

S

T

A

A

V

V

I

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

R

R

P

V

R
|
R

S

S

T

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

V

V

A

A

N

D

M

F

L

L

Q

S

A

S

V

V

G

G

V

V

A

D

R

R

V

V

L

L

T

T

M

V

D

D

L

L

H
|
H

A

A

D
x
E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

V

V

D

D

N

N

I

V

Y
x
F

A

G

S
|
S

P

P

V

I

L

L

G

E

D

D

L

M

R

L

Q

Q

K

L

N

N

Y

L

E

D

D

N

L

P

I

I

V

V

S

S

P

P

D
|
D

V

I

G

G

V

V

V
|
V

R

R

A

A

R

R

A

A

L

I

A

A

K

K

Q

L

L

L

G

N

-

D

C

T

D

D

L

M

A

A

I

I

D

D

K

K

R

R

P

-

K

-

A

-

N

-

V

-

S

-

E

Q

V

V

M

M

H

H

V

I

I

I

G

G

E

D

I

V

E

A

G

G

R

R

N

D

C

C

V

V

I

L

M

V

D
|
D

D

D

M
|
M

I

I

D
|
D

T
|
T

A

G

G

G

T

T

L

L

V

C

K

K

A

A

E

E

V

A

L

L

K

K

E

E

R

R

G

G

A

A

K

K

S

R

V

V

Y

F

A

A

Y

Y

C

A

T

T

H

H

P

P

I

I

F

F

S

S

G

G

P

N

A

A

I

A

E

N

R

N

I

L

A

-

K

R

G

N

S

S

A

V

L

I

D

D

E

E

V

V

T

C

N

D

T

T

I

I

P

P

L

L

S

S

D

D

N

E

A

I

K

K

G

S

C

L

T

P

K

N

I

V

R

R

Q

T

L

L

S

T

V

L

A

S

P

G

L

M

I

L

A

A

E

E

T

A

I

I

Q

R

R

R

I

I

A

S

K

N

G

E

E

E

S

S

V

I

M

S

S

A

L

M

F

F

binding adenosine-5'-diphosphate: F33 (= F39), D35 (= D41), E37 (= E43), R94 (= R100), Q95 (= Q101), R97 (= R103), R97 (= R103), R100 (= R106), H129 (= H135), E131 (≠ D137), F145 (≠ Y151), S147 (= S153), V173 (= V179)
binding 5-O-phosphono-alpha-D-ribofuranose: D168 (= D174), D212 (= D223), M214 (= M225), D216 (= D227), T217 (= T228)

6nfeB Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
64% identity, 93% coverage: 8:307/324 of query aligns to 3:297/299 of 6nfeB

query
sites
6nfeB

F

M

M

M

V

I

F

F

T

T

G

G

N

N

A

A

N

N

P

P

G

E

M

L

A

A

A

L

E

K

I

I

A

S

Q

S

H

H

L

L

G

Q

T

I

T

P

L

I

G

G

A

K

A

A

D

T

V

V

G

G

R

T

F
|
F

S

S

D
|
D

G

G

E
|
E

V

T

T

M

V

V

E

E

I

I

K

L

Q

E

N

N

V

V

R

R

A

G

R

K

D

D

V

V

F

F

V

V

V

L

Q

Q

S

S

T

T

C

C

A

A

P

P

T

A

N

N

E

N

N

N

L

L

M

M

E

E

L

L

I

I

M

M

V

A

D

D

A

A

L

L

K

R

R

R

A

S

S

S

A

A

E

G

R

R

I

I

S

T

A

A

V

V

I

V

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

P

V

R

R

S

S

T

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

V

V

A

A

N

D

M

M

L

M

Q

A

A

S

V

V

G

G

V

I

A

C

R

R

V

V

L

L

T

T

M

V

D

D

L

L

H
|
H

A

A

D

D

Q

Q

I

I

Q

Q

G

G

F

F

D

Y

I

M

P

P

V

V

D

D

N

N

I

V

Y

Y

A

S

S

T

P

P

V

V

L

L

G

E

D

D

L

I

R

T

Q

K

K

Q

N

K

Y

L

E

N

D

N

L

I

I

M

V

I

V

V

S

S

P

P

D

D

V

V

G

G

V

V

R

R

A

A

R

R

A

A

L

V

A

A

K

K

Q

R

L

L

G

N

-

D

C

A

D

E

L

L

A

S

I

I

D

D

K

K

R

R

P

-

K

-

A

-

N

-

V

-

S

S

E

E

V

V

M

M

H

H

V

I

I

I

G

G

E

E

I

P

E

A

G

N

R

K

N

N

C

C

V

I

I

I

M

V

D
|
D

M
x
I

I

V

D
|
D

T
|
T

A
|
A

G

G

T
|
T

L

L

V

C

K

T

A

A

E

H

V

E

L

L

K

K

E

K

R

N

G

G

A

A

K

K

S

S

V

V

Y

R

A

A

Y

Y

C

I

T

T

H

H

P

P

I

V

F

L

S

S

G

G

P

P

A

A

I

V

E

N

R

N

I

I

A

-

K

K

G

H

S

S

A

G

L

L

D

D

E

E

V

V

T

T

N

D

T

T

I

I

P

P

L

L

S

S

D

A

N

E

A

A

K

Q

G

N

C

C

T

E

K

K

I

I

R

R

Q

V

L

V

S

S

V

L

A

A

P

D

L

M

I

L

A

A

E

Q

T

A

I

I

Q

K

R

R

I

V

binding adenosine-5'-diphosphate: F34 (= F39), D36 (= D41), E38 (= E43), R95 (= R100), Q96 (= Q101), H130 (= H135)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H135), D214 (= D223), D215 (= D224), I216 (≠ M225), D218 (= D227), T219 (= T228), A220 (= A229), T222 (= T231)

6nfeA Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
63% identity, 93% coverage: 8:307/324 of query aligns to 3:296/298 of 6nfeA

query
sites
6nfeA

F

M

M

M

V

I

F

F

T

T

G

G

N

N

A

A

N

N

P

P

G

E

M

L

A

A

A

L

E

K

I

I

A

S

Q

S

H

H

L

L

G

Q

T

I

T

P

L

I

G

G

A

K

A

A

D

T

V

V

G

G

R

T

F
|
F

S

S

D
|
D

G

G

E
|
E

V

T

T

M

V

V

E

E

I

I

K

L

Q

E

N

N

V

V

R

R

A

G

R

K

D

D

V

V

F

F

V

V

V

L

Q

Q

S

S

T

T

C

C

A

A

P

P

T

A

N

N

E

N

N

N

L

L

M

M

E

E

L

L

I

I

M

M

V

A

D

D

A

A

L

L

K

R

R

R

A

S

S

S

A

A

E

G

R

R

I

I

S

T

A

A

V

V

I

V

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

R

R

P
x
V

R

R

S

S

T

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

V

V

A

A

N

D

M

M

L

M

Q

A

A

S

V

V

G

G

V

I

A

C

R

R

V

V

L

L

T

T

M

V

D

D

L

L

H
|
H

A

A

D

D

Q

Q

I

I

Q

Q

G

G

F

F

D

Y

I

M

P

P

V

V

D

D

N

N

I

V

Y
|
Y

A

S

S

T

P

P

V

V

L

L

G

E

D

D

L

I

R

T

Q

K

K

Q

N

K

Y

L

E

N

D

N

L

I

I

M

V

I

V

V

S

S

P

P

D

D

V

V

G

G

V

V

R
|
R

A

A

R

R

A
|
A

L

V

A

A

K
|
K

Q

R

L

L

G

N

-

D

C

A

D

E

L

L

A

S

I

I

D

D

K

K

R

R

P

-

K

-

A

-

N

-

V

-

S

-

E

E

V

V

M

M

H

H

V

I

I

I

G

G

E

E

I

P

E

A

G

N

R

K

N

N

C

C

V

I

I

I

M

V

D
|
D

M
x
I

I

V

D
|
D

T
|
T

A
|
A

G

G

T
|
T

L

L

V

C

K

T

A

A

E

H

V

E

L

L

K

K

E

K

R

N

G

G

A

A

K

K

S

S

V

V

Y

R

A

A

Y

Y

C

I

T

T

H

H

P

P

I

V

F

L

S

S

G

G

P

P

A

A

I

V

E

N

R

N

I

I

A

-

K

K

G

H

S

S

A

G

L

L

D

D

E

E

V

V

T

T

N

D

T

T

I

I

P

P

L

L

S

S

D

A

N

E

A

A

K

Q

G

N

C

C

T

E

K

K

I

I

R

R

Q

V

L

V

S

S

V

L

A

A

P

D

L

M

I

L

A

A

E

Q

T

A

I

I

Q

K

R

R

I

V

binding adenosine-5'-diphosphate: F34 (= F39), D36 (= D41), E38 (= E43), R95 (= R100), Q96 (= Q101), H130 (= H135)
binding adenosine monophosphate: R98 (= R103), V100 (≠ P105), Y146 (= Y151), R175 (= R180), A178 (= A183), K181 (= K186)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H135), D213 (= D223), D214 (= D224), I215 (≠ M225), D217 (= D227), T218 (= T228), A219 (= A229), T221 (= T231)

P14193 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PPRibP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Bacillus subtilis (strain 168) (see 4 papers)
55% identity, 98% coverage: 2:318/324 of query aligns to 4:317/317 of P14193

query
sites
P14193

Q

Q

A

Y

N

G

H

D

P

K

D

N

F

L

M

K

V

I

F

F

T

S

G

L

N

N

A

S

N

N

P

P

G

E

M

L

A

A

A

K

E

E

I

I

A

A

Q

D

H

I

L

V

G

G

T

V

T

Q

L

L

G

G

A

K

A

C

D

S

V

V

G

T

R

R

F

F

S

S

D

D

G

G

E

E

V

V

T

Q

V

I

E

N

I

I

K

E

Q

E

N

S

V

I

R

R

A

G

R

C

D

D

V

C

F

Y

V

I

Q

Q

S

S

T

T

C

S

A

D

P

P

T

V

N

N

E

E

N

H

L

I

M

M

E

E

L

L

I

I

M

M

V

V

D

D

A

A

L

L

K

K

R

R

A

A

S

S

A

A

E

K

R

T

I

I

S

N

A

I

V

V

I

I

P

P

Y

Y

F

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

R

K

P

A

R

R

S

S

T

-

R

R

V

E

P

P

I

I

T

T

A

A

K

K

V

L

V

F

A

A

N

N

M

L

L

L

Q

E

A

T

V

A

G

G

V

A

A

T

R

R

V

V

L

I

T

A

M

L

D

D

L

L

H

H

A

A

D

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

I

D

D

N

H

I

L

Y

M

A

G

S

V

P

P

V

I

L

L

L

G

G

E

D

Y

L

F

R

E

Q

G

K

K

N

N

Y

L

E

E

D

D

L

I

I

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

V

T

R

R

A

A

R

R

A

K

L

L

A

A

K

D

Q

R

L

L

G

K

C

A

D

P

L

I

A

A

I

I

D

D

K
|
K

R

R

R
|
R

P

P

K
x
R

A

P

N
|
N

V

V

S

A

E
|
E

V

V

M

M

H

N

V

I

I

V

G

G

E

N

I

I

E

E

G

G

R

K

N

T

C

A

V

I

I

L

M

I

D

D

M

I

I

I

D
|
D

T
|
T

A
|
A

G
|
G

T
|
T

L

I

V

T

K

L

A

A

E

N

V

A

L

L

K

V

E

E

R

N

G

G

A

A

K

K

S

E

V

V

Y

Y

A

A

Y

C

C

C

T

T

H

H

P

P

I

V

F

L

S

S

G

G

P

P

A

A

I

V

E

E

R

R

I

I

A

-

K

N

G

N

S

S

A

T

L

I

D

K

E

E

V

L

V

V

T

T

N

N

T

S

I

I

P

K

L

L

S

P

D

E

N

E

A

K

K

K

G

-

C

I

T

E

K

R

I

F

R

K

Q

Q

L

L

S

S

V

V

A

G

P

P

L

L

I

L

A

A

E

E

T

A

I

I

Q

I

R

R

I

V

A

H

K

E

G

Q

E

Q

S

S

V

V

M

S

S

Y

L

L

F

F

S

S

RQ 102:103 (= RQ 100:101) binding
K198 (= K197) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type. The cooperativity of ADP binding is reduced.
R200 (= R199) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type enzyme. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type.
R202 (≠ K201) mutation to A: 3-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
N204 (= N203) mutation to A: 4.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
E207 (= E206) mutation to A: 2.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
DTAGT 228:232 (= DTAGT 227:231) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1ibsB Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
52% identity, 96% coverage: 7:317/324 of query aligns to 3:299/299 of 1ibsB

query
sites
1ibsB

D

N

F

L

M

K

V

I

F

F

T

S

G

L

N

N

A

S

N

N

P

P

G

E

M

L

A

A

A

K

E

E

I

I

A

A

Q

D

H

I

L

V

G

G

T

V

T

Q

L

L

G

G

A

K

A

C

D

S

V

V

G

T

R

R

F

F

S

S

D

D

G

G

E

E

V

V

T

Q

V

I

E

N

I

I

K

E

Q

E

N

S

V

I

R

R

A

G

R

C

D

D

V

C

F

Y

V

I

Q

Q

S

S

T

T

C

S

A

D

P

P

T

V

N

N

E

E

N

H

L

I

M

M

E

E

L

L

I

I

M

M

V

V

D

D

A

A

L

L

K

K

R

R

A

A

S

S

A

A

E

K

R

T

I

I

S

N

A

I

V

V

I

I

P

P

Y

Y

F

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

R

K

P

S

R

R

S

E

T

-

R

-

V

-

P

P

I

I

T

T

A

A

K

K

V

L

V

F

A

A

N

N

M

L

L

L

Q

E

A

T

V

A

G

G

V

A

A

T

R

R

V

V

L

I

T

A

M

L

D

D

L

L

H

H

A

A

D

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

I

D

D

N

H

I

L

Y

M

A

G

S

V

P

P

V

I

L

L

L

G

G

E

D

Y

L

F

R

E

Q

G

K

K

N

N

Y

L

E

E

D

D

L

I

I

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

V

T

R

R

A

A

R

R

A

K

L

L

A

A

K

D

Q

R

L

L

G

K

C

A

D

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

P

-

K

-

A

-

N

-

V

-

S

-

E

-

V

-

M

M

H

N

V

I

I

V

G

G

E

N

I

I

E

E

G

G

R

K

N

T

C

A

V

I

I

L

M

I

D

D

M

I

I

I

D

D

T

T

A

A

G

G

T

T

L

I

V

T

K

L

A

A

E

N

V

A

L

L

K

V

E

E

R

N

G

G

A

A

K

K

S

E

V

V

Y

Y

A

A

Y

C

C

C

T

T

H

H

P

P

I

V

F

L

S

S

G

G

P

P

A

A

I

V

E

E

R

R

I

I

A

-

K

N

G

N

S

S

A

T

L

I

D

K

E

E

V

L

V

V

T

T

N

N

T

S

I

I

P

K

L

L

S

P

D

E

N

E

A

K

K

K

G

-

C

I

T

E

K

R

I

F

R

K

Q

Q

L

L

S

S

V

V

A

G

P

P

L

L

I

L

A

A

E

E

T

A

I

I

Q

I

R

R

I

V

A

H

K

E

G

Q

E

Q

S

S

V

V

M

S

S

Y

L

L

F

F

binding methyl phosphonic acid adenosine ester: R96 (= R100), Q97 (= Q101), R99 (= R103)

1ibsA Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
52% identity, 96% coverage: 7:317/324 of query aligns to 1:297/297 of 1ibsA

query
sites
1ibsA

D

N

F

L

M

K

V

I

F

F

T

S

G

L

N

N

A

S

N

N

P

P

G

E

M

L

A

A

A

K

E

E

I

I

A

A

Q

D

H

I

L

V

G

G

T

V

T

Q

L

L

G

G

A

K

A

C

D

S

V

V

G

T

R

R

F

F

S

S

D

D

G

G

E

E

V

V

T

Q

V

I

E

N

I

I

K

E

Q

E

N

S

V

I

R

R

A

G

R

C

D

D

V

C

F

Y

V

I

Q

Q

S

S

T

T

C

S

A

D

P

P

T

V

N

N

E

E

N

H

L

I

M

M

E

E

L

L

I

I

M

M

V

V

D

D

A

A

L

L

K

K

R

R

A

A

S

S

A

A

E

K

R

T

I

I

S

N

A

I

V

V

I

I

P

P

Y

Y

F

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

R

K

P

S

R

R

S

E

T

-

R

-

V

-

P

P

I

I

T

T

A

A

K

K

V

L

V

F

A

A

N

N

M

L

L

L

Q

E

A

T

V

A

G

G

V

A

A

T

R

R

V

V

L

I

T

A

M

L

D

D

L

L

H
|
H

A

A

D

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

I

D

D

N

H

I

L

Y

M

A

G

S

V

P

P

V

I

L

L

L

G

G

E

D

Y

L

F

R

E

Q

G

K

K

N

N

Y

L

E

E

D

D

L

I

I

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

V

T

R

R

A

A

R

R

A

K

L

L

A

A

K

D

Q

R

L

L

G

K

C

A

D

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

P

-

K

-

A

-

N

-

V

-

S

-

E

-

V

-

M

M

H

N

V

I

I

V

G

G

E

N

I

I

E

E

G

G

R

K

N

T

C

A

V

I

I

L

M

I

D

D

M

I

I

I

D
|
D

T
|
T

A
|
A

G

G

T
|
T

L

I

V

T

K

L

A

A

E

N

V

A

L

L

K

V

E

E

R

N

G

G

A

A

K

K

S

E

V

V

Y

Y

A

A

Y

C

C

C

T

T

H

H

P

P

I

V

F

L

S

S

G

G

P

P

A

A

I

V

E

E

R

R

I

I

A

-

K

N

G

N

S

S

A

T

L

I

D

K

E

E

V

L

V

V

T

T

N

N

T

S

I

I

P

K

L

L

S

P

D

E

N

E

A

K

K

K

G

-

C

I

T

E

K

R

I

F

R

K

Q

Q

L

L

S

S

V

V

A

G

P

P

L

L

I

L

A

A

E

E

T

A

I

I

Q

I

R

R

I

V

A

H

K

E

G

Q

E

Q

S

S

V

V

M

S

S

Y

L

L

F

F

binding methyl phosphonic acid adenosine ester: R94 (= R100), Q95 (= Q101), R97 (= R103), H126 (= H135)
binding sulfate ion: D209 (= D227), T210 (= T228), A211 (= A229), T213 (= T231)

1dkuA Crystal structures of bacillus subtilis phosphoribosylpyrophosphate synthetase: molecular basis of allosteric inhibition and activation. (see paper)
53% identity, 96% coverage: 7:317/324 of query aligns to 1:295/295 of 1dkuA

query
sites
1dkuA

D

N

F

L

M

K

V

I

F

F

T

S

G

L

N

N

A

S

N

N

P

P

G

E

M

L

A

A

A

K

E

E

I

I

A

A

Q

D

H

I

L

V

G

G

T

V

T

Q

L

L

G

G

A

K

A

C

D

S

V

V

G

T

R

R

F

F

S

S

D

D

G

G

E

E

V

V

T

Q

V

I

E

N

I

I

K

E

Q

E

N

S

V

I

R

R

A

G

R

C

D

D

V

C

F

Y

V

I

Q

Q

S

S

T

T

C

S

A

D

P

P

T

V

N

N

E

E

N

H

L

I

M

M

E

E

L

L

I

I

M

M

V

V

D

D

A

A

L

L

K

K

R

R

A

A

S

S

A

A

E

K

R

T

I

I

S

N

A

I

V

V

I

I

P

P

Y

Y

F

Y

G

G

Y

Y

A

A

R
|
R

Q

Q

D

D

R

R

R
x
K

P

A

R

R

S
|
S

T

-

R
|
R

V

E

P

P

I

I

T

T

A

A

K

K

V

L

V

F

A

A

N

N

M

L

L

L

Q

E

A

T

V

A

G

G

V

A

A

T

R

R

V

V

L

I

T

A

M

L

D

D

L

L

H
|
H

A

A

D

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

I

D
|
D

N

H

I

L

Y

M

A

G

S

V

P

P

V

I

L

L

L

G

G

E

D

Y

L

F

R

E

Q

G

K

K

N

N

Y

L

E

E

D

D

L

I

I

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

V

T

R

R

A

A

R

R

A

K

L

L

A

A

K

D

Q

R

L

L

G

K

C

A

D

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

P

-

K

-

A

-

N

-

V

-

S

-

E

-

V

-

M

M

H

N

V

I

I

V

G

G

E

N

I

I

E

E

G

G

R

K

N

T

C

A

V

I

I

L

M

I

D

D

M

I

I

I

D

D

T

T

A

A

G

G

T

T

L

I

V

T

K

L

A

A

E

N

V

A

L

L

K

V

E

E

R

N

G

G

A

A

K

K

S

E

V

V

Y

Y

A

A

Y

C

C

C

T

T

H

H

P

P

I

V

F

L

S

S

G

G

P

P

A

A

I

V

E

E

R

R

I

I

A

-

K

N

G

N

S

S

A

T

L

I

D

K

E

E

V

L

V

V

T

T

N

N

T

S

I

I

P

K

L

L

S

K

D

-

N

-

A

-

K

-

G

-

C

I

T

E

K

R

I

F

R

K

Q

Q

L

L

S

S

V

V

A

G

P

P

L

L

I

L

A

A

E

E

T

A

I

I

Q

I

R

R

I

V

A

H

K

E

G

Q

E

Q

S
|
S

V
|
V

M
x
S

S

Y

L

L

F
|
F

binding methyl phosphonic acid adenosine ester: R94 (= R100), H128 (= H135)
binding phosphomethylphosphonic acid adenosyl ester: K98 (≠ R104), S101 (= S107), R102 (= R109), D141 (= D148), S290 (= S312), V291 (= V313), S292 (≠ M314), F295 (= F317)

P60891 Ribose-phosphate pyrophosphokinase 1; PPRibP; Phosphoribosyl pyrophosphate synthase I; PRS-I; EC 2.7.6.1 from Homo sapiens (Human) (see 5 papers)
49% identity, 97% coverage: 6:318/324 of query aligns to 2:314/318 of P60891

query
sites
P60891

P

P

D

N

F

I

M

K

V

I

F

F

T

S

G

G

N

S

A

S

N

H

P

Q

G

D

M

L

A
x
S

A

Q

E

K

I

I

A

A

Q

D

H

R

L

L

G

G

T

L

T

E

L

L

G

G

A

K

A

V

D

V

V

T

G

K

R

K

F

F

S

S

D

N

G

Q

E

E

V

T

T

C

V

V

E

E

I

I

K

G

Q

E

N

S

V

V

R

R

A

G

R

E

D
|
D

V

V

F

Y

V

I

V

V

Q

Q

S

S

T

G

C

C

A

G

P

E

T

I

N

N

E

D

N

N

L

L

M

M

E

E

L

L

I

I

M

M

V

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

A

E

S

R

R

I

V

S

T

A

A

V

V

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R

R

Q

Q

D

D

R

K

P

D

R

K

S

S

T

-

R

R

V

A

P

P

I

I

T

S

A

A

K

K

V

L

V

V

A

A

N
|
N

M

M

L

L

Q

S

A

V

V

A

G

G

V

A

A

D

R

H

V

I

L

I

T

T

M

M

D

D

L
|
L

H

H

A

A

D
x
S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V
|
V

D

D

N
|
N

I

L

Y
|
Y

A

A

S

E

P

P

V

A

L

V

L

L

G

K

D

W

L

I

R

R

Q

E

K

N

-

I

-

S

N

E

Y

W

E

R

D

N

L

C

I

T

V

I

V

V

S

S

P

P

D

D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

L

I

A

A

K
x
D

Q

R

L

L

G

N

C

V

D

D

L

F

A
|
A

I

L

I

I

D
x
H

K

K

R

E

R

R

P

K

K

K

A

A

N

N

V

E

S

V

E
x
D

V

R

M

M

H

V

V

L

I

V

G

G

E

D

I

V

E

K

G

D

R

R

N

V

C

A

V

I

I

L

M
x
V

D

D

M

M

I

A

D

D

T

T

A

C

G

G

T

T

L

I

V

C

K
x
H

A

A

E

D

V

K

L

L

K

L

E

S

R

A

G

G

A

A

K

T

S

R

V

V

Y

Y

A

A

Y

I

C

L

T

T

H

H

P

G

I

I

F

F

S

S

G

G

P

P

A

A

I

I

E

S

R

R

I

I

A

-

K

N

G

N

S

A

A

C

L

F

D

E

E

A

V

V

T

T

N

N

T

T

I

I

P

P

L

Q

S

E

D

D

N

K

A

M

K

K

G

H

C

C

T

S

K

K

I

I

R

Q

Q

V

L

I

S

D

V

I

A

S

P

M

L

I

I

L

A

A

E

E

T

A

I

I

Q

R

R

R

I

T

A

H

K

N

G

G

E

E

S

S

V

V

M

S

S

Y

L

L

F

F

S

S

S16 (≠ A20) to P: found in patients with phosphoribosyl pyrophosphate synthetase I deficiency; likely pathogenic; dbSNP:rs869025594
D52 (= D56) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852542
N114 (= N119) to S: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852540
L129 (= L134) to I: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852543
S132 (≠ D137) mutation to A: Reduces catalytic activity.; mutation to F: No effect on catalytic activity.
V142 (= V147) to L: found in a patient with an intermediate phenotype between ARTS and PRPS1 superactivity; likely pathogenic; normal PRPP synthetase activity in fibroblasts; loss of activity in erythrocytes; dbSNP:rs398122855
N144 (= N149) mutation to H: No effect on catalytic activity.
Y146 (= Y151) mutation to F: No effect on catalytic activity.; mutation to M: Reduces catalytic activity.
D183 (≠ K186) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852541
A190 (= A193) to V: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852544
H193 (≠ D196) to Q: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852545
D203 (≠ E206) to H: in a breast cancer sample; somatic mutation
V219 (≠ M222) to G: in a breast cancer sample; somatic mutation
H231 (≠ K234) to D: in a colorectal cancer sample; somatic mutation

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

8dbkB Human prps1 with phosphate, atp, and r5p; hexamer with resolved catalytic loops (see paper)
49% identity, 97% coverage: 6:318/324 of query aligns to 1:313/316 of 8dbkB

query
sites
8dbkB

P

P

D

N

F

I

M

K

V

I

F

F

T

S

G

G

N

S

A

S

N

H

P

Q

G

D

M

L

A

S

A

Q

E

K

I

I

A

A

Q

D

H

R

L

L

G

G

T

L

T

E

L

L

G

G

A

K

A

V

D

V

V

T

G

K

R

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

V

T

T

C

V

V

E

E

I

I

K

G

Q

E

N
x
S

V

V

R
|
R

A

G

R

E

D

D

V

V

F

Y

V

I

V

V

Q

Q

S

S

T

G

C

C

A

G

P

E

T

I

N

N

E

D

N

N

L

L

M

M

E

E

L

L

I

I

M

M

V

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

A

E

S

R

R

I

V

S

T

A

A

V

V

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

K

P

D

R

K

S

S

T

-

R

R

V

A

P

P

I

I

T

S

A

A

K

K

V

L

V

V

A

A

N

N

M

M

L

L

Q

S

A

V

V

A

G

G

V

A

A

D

R

H

V

I

L

I

T

T

M

M

D

D

L

L

H
|
H

A

A

D

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D

D

N

N

I

L

Y

Y

A

A

S

E

P

P

V

A

L

V

L

L

G

K

D

W

L

I

R

R

Q

E

K

N

-

I

-

S

N

E

Y

W

E

R

D

N

L

C

I

T

V

I

V

V

S

S

P

P

D
|
D

V

A

G
|
G

G

G

V

A

V

K

R

R

A

V

R

T

A

S

L

I

A

A

K

D

Q

R

L

L

G

N

C

V

D

D

L

F

A

A

I

L

I

I

D

H

K
|
K

R

E

R
|
R

P

K

K

K

A

A

N
|
N

V

E

S

V

E

D

V

R

M

M

H

V

V

L

I

V

G

G

E

D

I

V

E

K

G

D

R

R

N

V

C

A

V

I

I

L

M

V

D
|
D

M

M

I

A

D
|
D

T
|
T

A
x
C

G
|
G

T
|
T

L

I

V

C

K

H

A

A

E

D

V

K

L

L

K

L

E

S

R

A

G

G

A

A

K

T

S

R

V

V

Y

Y

A

A

Y

I

C

L

T

T

H

H

P

G

I

I

F

F

S

S

G

G

P

P

A

A

I

I

E

S

R

R

I

I

A

-

K

N

G

N

S

A

A

C

L

F

D

E

E

A

V

V

T

T

N

N

T

T

I

I

P

P

L

Q

S

E

D

D

N

K

A

M

K

K

G

H

C

C

T

S

K

K

I

I

R

Q

Q

V

L

I

S

D

V

I

A

S

P

M

L

I

I

L

A

A

E

E

T

A

I

I

Q

R

R

R

I

T

A

H

K

N

G

G

E

E

S

S

V

V

M

S

S

Y

L

L

F

F

S

S

binding adenosine monophosphate: R95 (= R100), Q96 (= Q101), N199 (= N203)
binding adenosine-5'-triphosphate: F34 (= F39), N36 (≠ D41), E38 (= E43)
binding phosphate ion: S46 (≠ N51), R48 (= R53)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: H129 (= H135), D170 (= D174), G172 (= G176), K193 (= K197), R195 (= R199), D219 (= D223), D220 (= D224), D223 (= D227), T224 (= T228), C225 (≠ A229), G226 (= G230), T227 (= T231)

8dbeA Human prps1 with adp; hexamer (see paper)
49% identity, 97% coverage: 6:318/324 of query aligns to 1:313/316 of 8dbeA

query
sites
8dbeA

P

P

D

N

F

I

M

K

V

I

F

F

T

S

G

G

N

S

A

S

N

H

P

Q

G

D

M

L

A

S

A

Q

E

K

I

I

A

A

Q

D

H

R

L

L

G

G

T

L

T

E

L

L

G

G

A

K

A

V

D

V

V

T

G

K

R

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

V

T

T

C

V

V

E

E

I

I

K

G

Q

E

N

S

V

V

R

R

A

G

R

E

D

D

V

V

F

Y

V

I

V

V

Q

Q

S

S

T

G

C

C

A

G

P

E

T

I

N

N

E

D

N

N

L

L

M

M

E

E

L

L

I

I

M

M

V

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

A

E

S

R

R

I

V

S

T

A

A

V

V

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

P
x
D

R

K

S
|
S

T

-

R
|
R

V

A

P

P

I

I

T

S

A

A

K

K

V

L

V

V

A

A

N

N

M

M

L

L

Q

S

A

V

V

A

G

G

V

A

A

D

R

H

V

I

L

I

T

T

M

M

D

D

L

L

H
|
H

A

A

D

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D
|
D

N

N

I

L

Y
|
Y

A

A

S

E

P

P

V

A

L

V

L

L

G

K

D

W

L

I

R

R

Q

E

K

N

-

I

-

S

N

E

Y

W

E

R

D

N

L

C

I

T

V

I

V

V

S

S

P

P

D
|
D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

L

I

A

A

K

D

Q

R

L

L

G

N

C

V

D

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

P

K

K

K

A

A

N

N

V

E

S

V

E

D

V

R

M

M

H

V

V

L

I

V

G

G

E

D

I

V

E

K

G

D

R

R

N

V

C

A

V

I

I

L

M

V

D
|
D

M

M

I

A

D
|
D

T
|
T

A

C

G
|
G

T
|
T

L

I

V

C

K

H

A

A

E

D

V

K

L

L

K

L

E

S

R

A

G

G

A

A

K

T

S

R

V

V

Y

Y

A

A

Y

I

C

L

T

T

H

H

P

G

I

I

F

F

S

S

G

G

P

P

A

A

I

I

E

S

R

R

I

I

A

-

K

N

G

N

S

A

A

C

L

F

D

E

E

A

V

V

T

T

N

N

T

T

I

I

P

P

L

Q

S

E

D

D

N

K

A

M

K

K

G

H

C

C

T

S

K

K

I

I

R

Q

Q

V

L

I

S

D

V

I

A

S

P

M

L

I

I

L

A

A

E

E

T

A

I

I

Q

R

R

R

I

T

A

H

K

N

G

G

E

E

S
|
S

V
|
V

M
x
S

S

Y

L

L

F
|
F

S

S

binding adenosine-5'-diphosphate: F34 (= F39), N36 (≠ D41), E38 (= E43), R95 (= R100), Q96 (= Q101), K98 (≠ R103), K99 (≠ R104), D100 (≠ P105), S102 (= S107), R103 (= R109), H129 (= H135), D142 (= D148), Y145 (= Y151), S307 (= S312), V308 (= V313), S309 (≠ M314), F312 (= F317)
binding 5-O-phosphono-alpha-D-ribofuranose: H129 (= H135), D170 (= D174), D219 (= D223), D220 (= D224), D223 (= D227), T224 (= T228), G226 (= G230), T227 (= T231)

O94413 Ribose-phosphate pyrophosphokinase 2; Phosphoribosyl pyrophosphate synthase 2; EC 2.7.6.1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
49% identity, 95% coverage: 10:318/324 of query aligns to 8:317/321 of O94413

query
sites
O94413

V

I

F

F

T

A

G

G

N

N

A

S

N

H

P

P

G

E

M

L

A

A

A

E

E

K

I

V

A

A

Q

R

H

R

L

I

G

G

T

L

T

S

L

L

G

G

A

K

A

V

D

A

V

V

G

V

R

Q

F

Y

S

S

D

N

G

R

E

E

V

T

T

S

V

V

E

T

I

I

K

G

Q

E

N

S

V

V

R

R

A

D

R

E

D

D

V

V

F

F

V

I

V

L

Q

Q

S

T

T

G

C

C

A

G

P

S

T

I

N

N

E

D

N

H

L

L

M

M

E

E

L

L

I

I

M

M

V

I

D

N

A

A

L

C

K

R

R

S

A

A

S

S

A

A

E

R

R

R

I

I

S

T

A

A

V

I

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R

R

Q

Q

D

D

R

K

P

D

R

K

S

S

T

-

R

R

V

A

P

P

I

I

T

T

A

A

K

R

V

L

V

V

A

A

N

N

M

M

L

L

Q

Q

A

T

V

A

G

G

V

C

A

N

R

H

V

I

L

I

T

T

M

M

D

D

L

L

H

H

A

A

D

S

Q

Q

I

I

Q

Q

G

G

F

F

D

N

I

V

P

P

V

V

D

D

N

N

I

L

Y

Y

A

A

S

E

P

P

V

S

L

V

L

L

G

R

D

Y

L

I

R

R

Q

E

K

N

-

I

-

D

-

T

N

T

Y

V

E

N

D

P

L

T

I

V

V

I

V

V

S
|
S

P

P

D

D

V

A

G

G

V

A

V

K

R

R

A

A

R

T

A

A

L

L

A

A

K

D

Q

R

L

L

G

D

C

L

D

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

P

Q

K

K

A

A

N

N

V

E

S

V

E

S

V

R

M

M

H

V

V

L

I

V

G

G

E

D

I

V

E

R

G

D

R

K

N

L

C

A

V

I

I

L

M

V

D

D

M

M

I

A

D

D

T

T

A

C

G

G

T

T

L

L

V

G

K

L

A

A

E

K

V

T

L

L

K

K

E

D

R

N

G

G

A

A

K

K

S

A

V

V

Y

Y

A

A

Y

I

C

V

T

T

H

H

P

G

I

I

F

L

S

S

G

G

P

K

A

A

I

I

E

K

R

V

I

I

A

N

K

E

G

-

S

S

A

A

L

L

D

E

E

K

V

V

V

I

V

V

T

T

N

N

T

T

I

I

P

P

L

H

S

D

D

D

N

K

A

R

K

S

G

L

C

C

T

S

K

K

I

I

R

E

Q

T

L

I

S

D

V

I

A

S

P

G

L

V

I

L

A

A

E

E

T

C

I

I

Q

R

R

R

I

I

A

H

K

H

G

G

E

E

S

S

V

V

M

S

S

V

L

L

F

F

S

S

S172 (= S172) modified: Phosphoserine

7yk1A Structural basis of human prps2 filaments (see paper)
47% identity, 97% coverage: 6:318/324 of query aligns to 1:304/306 of 7yk1A

query
sites
7yk1A

P

P

D

N

F

I

M

V

V

L

F

F

T

S

G

G

N

S

A

S

N

H

P

Q

G

D

M

L

A

S

A

Q

E

R

I

V

A

A

Q

D

H

R

L

L

G

G

T

L

T

E

L

L

G

G

A

K

A

V

D

V

V

T

G

K

R

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

V

T

T

S

V

V

E

E

I

I

K

G

Q

E

N
x
S

V

V

R
|
R

A

G

R

E

D

D

V

V

F

Y

V

I

Q

Q

S

S

T

G

C

C

A

G

P

E

T

I

N

N

E

D

N

N

L

L

M

M

E

E

L

L

I

I

M

M

V

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

S

E

S

R

R

I

V

S

T

A

A

V

V

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R
|
R

Q

Q

D

D

R

K

R
x
K

P
x
D

R
x
K

S
|
S

T

-

R
|
R

V

A

P

P

I

I

T

S

A

A

K

K

V

L

V

V

A

A

N

N

M

M

L

L

Q

S

A

V

V

A

G

G

V

A

A

D

R

H

V

I

L

I

T

T

M

M

D

D

L

L

H
|
H

A

A

D

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D
|
D

N

N

I

L

Y

Y

A

A

S

E

P

P

V

A

L

V

L

L

G

Q

D

W

L

I

R

R

Q

E

K

N

-

I

-

A

N

E

Y

W

E

K

D

N

L

C

I

I

V

I

V

V

S

S

P

P

D

D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

L

I

A

A

K

D

Q

R

L

L

G

N

C

V

D

E

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

-

P

-

K

-

A

-

N

-

V

-

S

-

E

-

V

-

M

M

H

V

V

L

I

V

G

G

E

D

I

V

E

K

G

D

R

R

N

V

C

A

V

I

I

L

M

V

D

D

M

M

I

A

D
|
D

T

T

A
x
C

G

G

T
|
T

L

I

V

C

K

H

A

A

E

D

V

K

L

L

K

L

E

S

R

A

G

G

A

A

K

T

S

K

V

V

Y

Y

A

A

Y

I

C

L

T

T

H

H

P

G

I

I

F

F

S

S

G

G

P

P

A

A

I

I

E

S

R

R

I

I

A

-

K

N

G

N

S

A

A

A

L

F

D

E

E

A

V

V

T

T

N

N

T

T

I

I

P

P

L

Q

S

E

D

D

N

K

A

M

K

K

G

H

C

C

T

T

K

K

I

I

R

Q

Q

V

L

I

S

D

V

I

A

S

P

M

L

I

I

L

A

A

E

E

T

A

I

I

Q

R

R

R

I

T

A

H

K

N

G

G

E

E

S
|
S

V

V

M
x
S

S

Y

L

L

F
|
F

S

S

binding adenosine-5'-diphosphate: F34 (= F39), N36 (≠ D41), E38 (= E43), S46 (≠ N51), R48 (= R53), R95 (= R100), K99 (≠ R104), D100 (≠ P105), K101 (≠ R106), S102 (= S107), R103 (= R109), H129 (= H135), D142 (= D148), S298 (= S312), S300 (≠ M314), F303 (= F317)
binding phosphate ion: D214 (= D227), C216 (≠ A229), T218 (= T231)

8dbgA Human prps1 with phosphate and atp; hexamer (see paper)
47% identity, 97% coverage: 6:318/324 of query aligns to 1:306/309 of 8dbgA

query
sites
8dbgA

P

P

D

N

F

I

M

K

V

I

F

F

T

S

G

G

N

S

A

S

N

H

P

Q

G

D

M

L

A

S

A

Q

E

K

I

I

A

A

Q

D

H

R

L

L

G

G

T

L

T

E

L

L

G

G

A

K

A

V

D

V

V

T

G

K

R

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

V

T

T

C

V

V

E

E

I

I

K

G

Q

E

N
x
S

V

V

R
|
R

A

G

R

E

D

D

V

V

F

Y

V

I

V

V

Q

Q

S

S

T

G

C

C

A

G

P

E

T

I

N

N

E

D

N

N

L

L

M

M

E

E

L

L

I

I

M

M

V

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

A

E

S

R

R

I

V

S

T

A

A

V

V

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

R

K

P

D

R

K

S

S

T

-

R

R

V

A

P

P

I

I

T

S

A

A

K

K

V

L

V

V

A

A

N

N

M

M

L

L

Q

S

A

V

V

A

G

G

V

A

A

D

R

H

V

I

L

I

T

T

M

M

D

D

L

L

H
|
H

A

A

D

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D

D

N

N

I

L

Y

Y

A

A

S

E

P

P

V

A

L

V

L

L

G

K

D

W

L

I

R

R

Q

E

K

N

-

I

-

S

N

E

Y

W

E

R

D

N

L

C

I

T

V

I

V

V

S

S

P

P

D

D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

L

I

A

A

K

D

Q

R

L

L

G

N

C

V

D

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

-

P

-

K

-

A

-

N

-

V

-

S

-

E

D

V

R

M

M

H

V

V

L

I

V

G

G

E

D

I

V

E

K

G

D

R

R

N

V

C

A

V

I

I

L

M

V

D

D

M

M

I

A

D
|
D

T
|
T

A
x
C

G

G

T
|
T

L

I

V

C

K

H

A

A

E

D

V

K

L

L

K

L

E

S

R

A

G

G

A

A

K

T

S

R

V

V

Y

Y

A

A

Y

I

C

L

T

T

H

H

P

G

I

I

F

F

S

S

G

G

P

P

A

A

I

I

E

S

R

R

I

I

A

-

K

N

G

N

S

A

A

C

L

F

D

E

E

A

V

V

T

T

N

N

T

T

I

I

P

P

L

Q

S

E

D

D

N

K

A

M

K

K

G

H

C

C

T

S

K

K

I

I

R

Q

Q

V

L

I

S

D

V

I

A

S

P

M

L

I

I

L

A

A

E

E

T

A

I

I

Q

R

R

R

I

T

A

H

K

N

G

G

E

E

S

S

V

V

M

S

S

Y

L

L

F

F

S

S

binding adenosine-5'-triphosphate: F34 (= F39), N36 (≠ D41), E38 (= E43), R95 (= R100), Q96 (= Q101), K98 (≠ R103), H129 (= H135)
binding phosphate ion: S46 (≠ N51), R48 (= R53), D216 (= D227), T217 (= T228), C218 (≠ A229), T220 (= T231)

2hcrA Crystal structure of human phosphoribosyl pyrophosphate synthetase 1 in complex with amp(atp), cadmium and sulfate ion (see paper)
48% identity, 95% coverage: 10:317/324 of query aligns to 4:305/305 of 2hcrA

query
sites
2hcrA

V

I

F

F

T

S

G

G

N

S

A

S

N

H

P

Q

G

D

M

L

A

S

A

Q

E

K

I

I

A

A

Q

D

H

R

L

L

G

G

T

L

T

E

L

L

G

G

A

K

A

V

D

V

V

T

G

K

R

K

F

F

S

S

D

N

G

Q

E

E

V

T

T

C

V

V

E

E

I

I

K

G

Q

E

N

S

V

V

R

R

A

G

R

E

D

D

V

V

F

Y

V

I

V

V

Q

Q

S

S

T

G

C

C

A

G

P

E

T

I

N

N

E

D

N

N

L

L

M

M

E

E

L

L

I

I

M

M

V

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

A

E

S

R

R

I

V

S

T

A

A

V

V

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

R

K

P

D

R

K

S

S

T

-

R

R

V

A

P

P

I

I

T

S

A

A

K

K

V

L

V

V

A

A

N

N

M

M

L

L

Q

S

A

V

V

A

G

G

V

A

A

D

R

H

V

I

L

I

T

T

M

M

D

D

L

L

H
|
H

A

A

D

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D

D

N

N

I

L

Y

Y

A

A

S

E

P

P

V

A

L

V

L

L

G

K

D

W

L

I

R

R

Q

E

K

N

-

I

-

S

N

E

Y

W

E

R

D

N

L

C

I

T

V

I

V

V

S

S

P

P

D

D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

A

S

L

I

A

A

K

D

Q

R

L

L

G

N

C

V

D

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

P

D

K

R

A

-

N

-

V

-

S

-

E

-

V

-

M

M

H

V

V

L

I

V

G

G

E

D

I

V

E

K

G

D

R

R

N

V

C

A

V

I

I

L

M

V

D

D

M

M

I

A

D

D

T

T

A

C

G

G

T

T

L

I

V

C

K

H

A

A

E

D

V

K

L

L

K

L

E

S

R

A

G

G

A

A

K

T

S

R

V

V

Y

Y

A

A

Y

I

C

L

T

T

H

H

P

G

I

I

F

F

S

S

G

G

P

P

A

A

I

I

E

S

R

R

I

I

A

-

K

N

G

N

S

A

A

C

L

F

D

E

E

A

V

V

T

T

N

N

T

T

I

I

P

P

L

Q

S

E

D

D

N

K

A

M

K

K

G

H

C

C

T

S

K

K

I

I

R

Q

Q

V

L

I

S

D

V

I

A

S

P

M

L

I

I

L

A

A

E

E

T

A

I

I

Q

R

R

R

I

T

A

H

K

N

G

G

E

E

S

S

V

V

M

S

S

Y

L

L

F

F

binding adenosine monophosphate: R94 (= R100), Q95 (= Q101), K97 (≠ R103), H128 (= H135)

Query Sequence

>Ac3H11_3452 FitnessBrowser__acidovorax_3H11:Ac3H11_3452
MQANHPDFMVFTGNANPGMAAEIAQHLGTTLGAADVGRFSDGEVTVEIKQNVRARDVFVV
QSTCAPTNENLMELLIMVDALKRASAERISAVIPYFGYARQDRRPRSTRVPITAKVVANM
LQAVGVARVLTMDLHADQIQGFFDIPVDNIYASPVLLGDLRQKNYEDLIVVSPDVGGVVR
ARALAKQLGCDLAIIDKRRPKANVSEVMHVIGEIEGRNCVIMDDMIDTAGTLVKAAEVLK
ERGAKSVYAYCTHPIFSGPAIERIAKGSALDEVVVTNTIPLSDNAKGCTKIRQLSVAPLI
AETIQRIAKGESVMSLFSDQDNLF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory