SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ac3H11_37 FitnessBrowser__acidovorax_3H11:Ac3H11_37 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1wtcA Crystal structure of s.Pombe serine racemase complex with amppcp (see paper)
71% identity, 98% coverage: 8:324/324 of query aligns to 2:318/318 of 1wtcA

query
sites
1wtcA

L

L

P

P

T

T

Y

Y

D

D

D

D

V

V

V

A

A

S

A

A

A

S

G

E

R

R

I

I

A

K

G

K

V

F

A

A

H
x
N

R

K

T

T

P

P

V

V

L

L

T

T

S

S

R

S

T

T

V

V

N

N

E

K

E

E

F

F

G

V

A

A

Q

E

V

V

F

F

F

F

K

K

C

C

E

E

N

N

L

F

Q

Q

R
x
K

M
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M

G

G

A

A

F
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F

K
|
K

F

F

R

R

G

G

A

A

Y

L

N

N

A

A

L

L

A

S

Q

Q

F

L

S

N

P

E

A

A

Q

Q

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R

K

K

A

A

G

G

V

V

V

L

A

T

F

F

S

S

S
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S

G

G

N
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N

H

H

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A

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G

A

I

I

A

A

L

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A

S

A

A

R

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E

I

L

L

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G

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I

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K

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I

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M

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L

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D

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P

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E

A

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K

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A

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K

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K

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E

E

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L

F

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E

E

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G

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P

L

L

D

D

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A

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L

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F

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V

C

C

L

L

G
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G

G
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G

G
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G

L

L

L

L

S

S

G

G

S

S

A

A

L

L

A

A

T

A

R

R

A

H

L

F

S

A

P

P

Q

N

T

C

K

E

L

V

Y

Y

G

G

V

V

E
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E

P

P

A

E

A

A

G
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G

N

N

D
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D

G

G

Q

Q

Q

Q

S

S

F

F

R

R

S

K

G

G

S

S

I

I

V

V

H

H

I

I

D

D

T

T

P

P

T

K

T

T

I

I

A

A

D

D

G
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G

A

A

Q

Q

T

T

Q

Q

H

H

L

L

G

G

H

N

I

Y

T

T

F

F

A

S

I

I

I

I

R

K

R

E

D

K

V

V

D

D

D

D

I

I

L

L

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D

D

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E

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E

L

L

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I

D

D

A

C

M

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F

F

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Y

A

A

E

A

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R

M

M

K

K

L

I

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V

V

E
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E

P

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T
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T

G

G

C

C

L

L

G

S

F

F

A

A

A

A

A

A

R

R

A

A

M

M

Q

K

S

E

E

K

L

L

K

K

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N

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K

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R

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I

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S

G

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G

G

N

N

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V

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D

L

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A

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active site: K52 (= K58), S77 (= S83), E203 (= E209), G207 (= G213), D209 (= D215), G231 (= G237), I302 (= I308), S303 (= S309)
binding phosphomethylphosphonic acid adenylate ester: N20 (≠ H26), K47 (≠ R53), M48 (= M54), A109 (= A115), A110 (= A116), Y114 (= Y120)
binding magnesium ion: E203 (= E209), G207 (= G213), D209 (= D215)
binding pyridoxal-5'-phosphate: F51 (= F57), K52 (= K58), N79 (= N85), G178 (= G184), G179 (= G185), G180 (= G186), G181 (= G187), G231 (= G237), E276 (= E282), T278 (= T284), S303 (= S309)

1v71A Crystal structure of s.Pombe serine racemase
71% identity, 98% coverage: 8:324/324 of query aligns to 2:318/318 of 1v71A

query
sites
1v71A

L

L

P

P

T

T

Y

Y

D

D

D

D

V

V

V

A

A

S

A

A

A

S

G

E

R

R

I

I

A

K

G

K

V

F

A

A

H

N

R

K

T

T

P

P

V

V

L

L

T

T

S

S

R

S

T

T

V

V

N

N

E

K

E

E

F

F

G

V

A

A

Q

E

V

V

F

F

F

F

K

K

C

C

E

E

N

N

L

F

Q

Q

R

K

M

M

G

G

A

A

F
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F

K
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K

F

F

R

R

G

G

A

A

Y

L

N

N

A

A

L

L

A

S

Q

Q

F

L

S

N

P

E

A

A

Q

Q

R

R

K

K

A

A

G

G

V

V

V

L

A

T

F

F

S

S

S
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S

G

G

N
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H

H

A

A

Q

Q

G

A

I

I

A

A

L

L

A

S

A

A

R

K

E

I

L

L

G

G

I

I

P

P

A

A

T

K

I

I

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M

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P

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L

D

D

A

A

P

P

A

E

A

A

K

K

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V

A

A

A

A

T

T

Q

K

G

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Y

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G

G

A

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D

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L

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I

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D

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H

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K

E

E

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F

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E

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G

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P

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L

D

D

A

A

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L

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F

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V

C

C

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L

G
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G

G
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G

G
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G

G
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G

L

L

L

L

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S

G

G

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S

A

A

L

L

A

A

T

A

R

R

A

H

L

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C

K

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L

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Y

Y

G

G

V

V

E
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E

P

P

A

E

A

A

G
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G

N

N

D
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D

G

G

Q

Q

Q

Q

S

S

F

F

R

R

S

K

G

G

S

S

I

I

V

V

H

H

I

I

D

D

T

T

P

P

T

K

T

T

I

I

A

A

D

D

G
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G

A

A

Q

Q

T

T

Q

Q

H

H

L

L

G

G

H

N

I

Y

T

T

F

F

A

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I

I

I

R

K

R

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D

K

V

V

D

D

D

D

I

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L

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D

E

E

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E

L

L

V

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D

D

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M

L

R

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F

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A

A

E

A

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R

M

M

K

K

L

I

V

V

V

V

E
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E

P

P

T
|
T

G

G

C

C

L

L

G

S

F

F

A

A

A

A

A

A

R

R

A

A

M

M

Q

K

S

E

E

K

L

L

K

K

G

N

Q

K

R

R

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I

G

G

V

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L

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I

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S

G
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G

G

G

N

N

V

V

D

D

L

I

A

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Y

C

A

A

H

L

F

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L

A

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Q

active site: K52 (= K58), S77 (= S83), E203 (= E209), G207 (= G213), D209 (= D215), G231 (= G237), I302 (= I308), S303 (= S309)
binding magnesium ion: E203 (= E209), G207 (= G213), D209 (= D215)
binding pyridoxal-5'-phosphate: F51 (= F57), K52 (= K58), N79 (= N85), G178 (= G184), G179 (= G185), G180 (= G186), G181 (= G187), G231 (= G237), E276 (= E282), T278 (= T284), S303 (= S309), G304 (= G310)

O59791 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 4.3.1.17; EC 4.3.1.18; EC 5.1.1.18 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
71% identity, 98% coverage: 8:324/324 of query aligns to 7:323/323 of O59791

query
sites
O59791

L

L

P

P

T

T

Y

Y

D

D

D

D

V

V

V

A

A

S

A

A

A

S

G

E

R

R

I

I

A

K

G

K

V

F

A

A

H

N

R

K

T

T

P

P

V

V

L

L

T

T

S

S

R

S

T

T

V

V

N

N

E

K

E

E

F

F

G

V

A

A

Q

E

V

V

F

F

F

F

K

K

C

C

E

E

N

N

L

F

Q

Q

R

K

M

M

G

G

A

A

F

F

K

K

F

F

R

R

G

G

A

A

Y

L

N

N

A

A

L

L

A

S

Q

Q

F

L

S

N

P

E

A

A

Q

Q

R

R

K

K

A

A

G

G

V

V

V

L

A

T

F

F

S

S

S
|
S

G

G

N

N

H

H

A

A

Q

Q

G

A

I

I

A

A

L

L

A

S

A

A

R

K

E

I

L

L

G

G

I

I

P

P

A

A

T

K

I

I

L

I

M

M

P

P

Q

L

D

D

A

A

P

P

A

E

A

A

K

K

V

V

A

A

A

A

T

T

Q

K

G

G

Y

Y

G

G

A

G

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Q

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V

V

I

F

M

F

Y

D

D

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R

Y

Y

T

K

Q

D

D

D

R

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E

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E

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S

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E

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R

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E

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G

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L

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Y

Y

D

D

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A

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D

H

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V

L

L

A

A

G

G

Q

Q

G

G

T

T

A

A

A

A

K

K

E

E

L

L

F

F

E

E

E

E

V

V

G

G

P

P

L

L

D

D

A

A

F

L

F

F

V

V

C

C

L

L

G

G

G

G

G

G

G

G

L

L

L

L

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S

G

G

S

S

A

A

L

L

A

A

T

A

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R

A

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L

F

S

A

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P

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N

T

C

K

E

L

V

Y

Y

G

G

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V

E

E

P

P

A

E

A

A

G

G

N

N

D

D

G

G

Q

Q

Q

Q

S

S

F

F

R

R

S

K

G

G

S

S

I

I

V

V

H

H

I

I

D

D

T

T

P

P

T

K

T

T

I

I

A

A

D

D

G

G

A

A

Q

Q

T

T

Q

Q

H

H

L

L

G

G

H

N

I

Y

T

T

F

F

A

S

I

I

I

I

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K

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E

D

K

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V

D

D

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D

I

I

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L

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D

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E

L

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M

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F

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A

A

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A

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R

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M

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K

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I

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V

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E

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P

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T

G

G

C

C

L

L

G

S

F

F

A

A

A

A

A

A

R

R

A

A

M

M

Q

K

S

E

E

K

L

L

K

K

G

N

Q

K

R

R

V

I

G

G

V

I

L

I

I

I

S

S

G

G

G

G

N

N

V

V

D

D

L

I

A

E

R

R

F

Y

C

A

A

H

L

F

V

L

A

S

R

Q

S82 (= S83) mutation to A: Loss of racemase activity. Reduces D-serine dehydratase activity by 99%. Slightly reduced L-serine dehydratase activity.

2zr8A Crystal structure of modified serine racemase complexed with serine (see paper)
71% identity, 98% coverage: 8:324/324 of query aligns to 3:319/319 of 2zr8A

query
sites
2zr8A

L

L

P

P

T

T

Y

Y

D

D

D

D

V

V

V

A

A

S

A

A

A

S

G

E

R

R

I

I

A

K

G

K

V

F

A

A

H

N

R

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T

T

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P

V

V

L

L

T

T

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S

R

S

T

T

V

V

N

N

E

K

E

E

F

F

G

V

A

A

Q

E

V

V

F

F

F

F

K

K

C

C

E

E

N

N

L

F

Q

Q

R

K

M

M

G

G

A

A

F
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F

K
|
K

F

F

R

R

G

G

A

A

Y

L

N

N

A

A

L

L

A

S

Q

Q

F

L

S

N

P

E

A

A

Q

Q

R

R

K

K

A

A

G

G

V

V

V

L

A

T

F

F

S
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S

S
|
S

G

G

N
|
N

H
|
H

A

A

Q

Q

G

A

I

I

A

A

L

L

A

S

A

A

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K

E

I

L

L

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G

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M

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L

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D

A

A

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P

A

E

A

A

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K

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A

A

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A

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K

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A

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F

M

F

Y

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D

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R

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Y

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D

D

R
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R

E

E

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A

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E

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R

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E

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G

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L

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T

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P
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P

Y

Y

D

D

H

H

A

P

D

H

V

V

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L

A

A

G

G

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Q

G

G

T

T

A

A

A

A

K

K

E

E

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L

F

F

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E

E

E

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V

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G

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P

L

L

D

D

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A

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F

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C

L

L

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G

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G

G
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G
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G

L

L

L

L

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G

G

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S

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A

L

L

A

A

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A

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R

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A

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K

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L

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Y

G

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E
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E

P

P

A

E

A

A

G
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G

N

N

D
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D

G

G

Q

Q

Q

Q

S

S

F

F

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R

S

K

G

G

S

S

I

I

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H

I

I

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D

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P

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K

T

T

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I

A

A

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D

G
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G

A
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A

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Q

T
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T

Q

Q

H

H

L

L

G

G

H

N

I

Y

T

T

F

F

A

S

I

I

I

I

R

K

R

E

D

K

V

V

D

D

D

D

I

I

L

L

T

T

V

V

T

S

D

D

E

E

Q

E

L

L

V

I

D

D

A

C

M

L

R

K

F

F

F

Y

A

A

E

A

R

R

M

M

K

K

L

I

V

V

V

V

E
|
E

P

P

T
|
T

G

G

C

C

L

L

G

S

F

F

A

A

A

A

A

A

R

R

A

A

M

M

Q

K

S

E

E

K

L

L

K

K

G

N

Q

K

R

R

V

I

G

G

V

I

L

I

I
|
I

S
|
S

G

G

G

G

N

N

V

V

D

D

L

I

A

E

R

R

F

Y

C

A

A

H

L

F

V

L

A

S

R

Q

active site: K53 (= K58), S78 (= S83), E204 (= E209), G208 (= G213), D210 (= D215), G232 (= G237), I303 (= I308), S304 (= S309)
binding magnesium ion: E204 (= E209), G208 (= G213), D210 (= D215)
binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F57), K53 (= K58), S77 (= S82), S78 (= S83), N80 (= N85), H81 (= H86), P147 (= P152), G179 (= G184), G180 (= G185), G181 (= G186), G182 (= G187), G232 (= G237), E277 (= E282), T279 (= T284), S304 (= S309)
binding serine: S78 (= S83), R129 (= R134), D231 (= D236), G232 (= G237), A233 (= A238), Q234 (= Q239), T235 (= T240)

2zpuA Crystal structure of modified serine racemase from s.Pombe. (see paper)
71% identity, 98% coverage: 8:324/324 of query aligns to 3:319/319 of 2zpuA

query
sites
2zpuA

L

L

P

P

T

T

Y

Y

D

D

D

D

V

V

V

A

A

S

A

A

A

S

G

E

R

R

I

I

A

K

G

K

V

F

A

A

H

N

R

K

T

T

P

P

V

V

L

L

T

T

S

S

R

S

T

T

V

V

N

N

E

K

E

E

F

F

G

V

A

A

Q

E

V

V

F

F

F

F

K

K

C

C

E

E

N

N

L

F

Q

Q

R

K

M

M

G

G

A

A

F
|
F

K
|
K

F

F

R

R

G

G

A

A

Y

L

N

N

A

A

L

L

A

S

Q

Q

F

L

S

N

P

E

A

A

Q

Q

R

R

K

K

A

A

G

G

V

V

V

L

A

T

F

F

S
|
S

S
|
S

G

G

N
|
N

H
|
H

A

A

Q

Q

G

A

I

I

A

A

L

L

A

S

A

A

R

K

E

I

L

L

G

G

I

I

P

P

A

A

T

K

I

I

L

I

M

M

P

P

Q

L

D

D

A

A

P

P

A

E

A

A

K

K

V

V

A

A

A

A

T

T

Q

K

G

G

Y

Y

G

G

A

G

Q

Q

V

V

V

I

F

M

F

Y

D

D

R

R

Y

Y

T

K

Q

D

D

D

R

R

E

E

Q

K

L

M

T

A

R

K

D

E

L

I

A

S

E

E

R

R

H

E

G

G

L

L

T

T

L

I

I

I

P

P

P
|
P

Y

Y

D

D

H

H

A

P

D

H

V

V

L

L

A

A

G

G

Q

Q

G

G

T

T

A

A

A

A

K

K

E

E

L

L

F

F

E

E

E

E

V

V

G

G

P

P

L

L

D

D

A

A

F

L

F

F

V

V

C

C

L

L

G
|
G

G
|
G

G
|
G

G
|
G

L

L

L

L

S

S

G

G

S

S

A

A

L

L

A

A

T

A

R

R

A

H

L

F

S

A

P

P

Q

N

T

C

K

E

L

V

Y

Y

G

G

V

V

E
|
E

P

P

A

E

A

A

G
|
G

N

N

D
|
D

G

G

Q

Q

Q

Q

S

S

F

F

R

R

S

K

G

G

S

S

I

I

V

V

H

H

I

I

D

D

T

T

P

P

T

K

T

T

I

I

A

A

D

D

G
|
G

A

A

Q

Q

T

T

Q

Q

H

H

L

L

G

G

H

N

I

Y

T

T

F

F

A

S

I

I

I

I

R

K

R

E

D

K

V

V

D

D

D

D

I

I

L

L

T

T

V

V

T

S

D

D

E

E

Q

E

L

L

V

I

D

D

A

C

M

L

R

K

F

F

F

Y

A

A

E

A

R

R

M

M

K

K

L

I

V

V

V

V

E
|
E

P

P

T
|
T

G

G

C

C

L

L

G

S

F

F

A

A

A

A

A

A

R

R

A

A

M

M

Q

K

S

E

E

K

L

L

K

K

G

N

Q

K

R

R

V

I

G

G

V

I

L

I

I
|
I

S
|
S

G

G

G

G

N

N

V

V

D

D

L

I

A

E

R

R

F

Y

C

A

A

H

L

F

V

L

A

S

R

Q

active site: K53 (= K58), S78 (= S83), E204 (= E209), G208 (= G213), D210 (= D215), G232 (= G237), I303 (= I308), S304 (= S309)
binding magnesium ion: E204 (= E209), G208 (= G213), D210 (= D215)
binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F57), K53 (= K58), S77 (= S82), S78 (= S83), N80 (= N85), H81 (= H86), P147 (= P152), G179 (= G184), G180 (= G185), G181 (= G186), G182 (= G187), G232 (= G237), E277 (= E282), T279 (= T284), S304 (= S309)

A4F2N8 L-threo-3-hydroxyaspartate ammonia-lyase; L-threo-3-hydroxyaspartate dehydratase; L-THA DH; EC 4.3.1.16 from Pseudomonas sp. (see paper)
71% identity, 97% coverage: 8:322/324 of query aligns to 3:317/319 of A4F2N8

query
sites
A4F2N8

L

L

P

S

T

S

Y

Y

D

H

D

D

V

V

V

I

A

K

A

A

A

A

G

E

R

R

I

L

A

E

G

G

V

F

A

A

H

N

R

R

T

T

P

P

V

V

L

F

T

T

S

S

R

R

T

T

V

L

N

D

E

A

E

E

F

T

G

G

A

A

Q

Q

V

V

F

F

F

I

K

K

C

C

E

E

N

N

L

L

Q

Q

R

R

M

T

G

G

A

S

F

F

K
|
K

F

F

R

R

G

G

A

A

Y

F

N

N

A

A

L

L

A

S

Q

R

F

F

S

D

P

E

A

A

Q

Q

R

R

K

K

A

A

G

G

V

V

V

V

A

A

F

F

S

S

S

S

G

G

N

N

H

H

A

A

Q

Q

G

G

I

I

A

A

L

L

A

A

A

A

R

R

E

L

L

L

G

Q

I

M

P

P

A

A

T

T

I

I

L

V

M

M

P

P

Q

T

D

D

A

A

P

P

A

A

A

A

K

K

V

V

A

A

A

A

T

T

Q

R

G

E

Y

Y

G

G

A

A

Q

T

V

V

V

V

F

F

F

Y

D

D

R

R

Y

I

T

T

Q

E

D

D

R

R

E

E

Q

Q

L

I

T

G

R

R

D

T

L

L

A

A

E

E

R

Q

H

H

G

G

L

M

T

T

L

L

I

I

P

P

P

S

Y

Y

D

D

H

H

A

P

D

D

V

V

L

L

A

A

G

G

Q

Q

G

G

T

T

A

A

A

A

K

K

E

E

L

L

F

L

E

E

E

F

V

T

G

G

P

P

L

L

D

D

A

A

F

L

F

F

V

V

C

G

L

L

G

G

G

G

G

G

G

G

L

M

L

L

S

S

G

G

S

T

A

A

L

L

A

A

T

T

R

R

A

A

L

L

S

S

P

P

Q

D

T

C

K

L

L

L

Y

Y

G

G

V

V

E

E

P

P

A

E

A

A

G

G

N

N

D

D

G

G

Q

Q

Q

R

S

S

F

F

R

Q

S

T

G

G

S

S

I

I

V

V

H

H

I

I

D

D

T

T

P

P

T

A

T

T

I

I

A

A

D

D

G

G

A

A

Q

Q

T

T

Q

Q

H

H

L

L

G

G

H

N

I

H

T

T

F

F

A

P

I

I

I

I

R

R

R

E

D

N

V

V

D

N

D

D

I

I

L

L

T

T

V

V

T

S

D

D

E

A

Q

E

L

L

V

V

D

E

A

S

M

M

R

R

F

F

F

F

A

M

E

Q

R

R

M

M

K

K

L

M

V

V

V

V

E

E

P

P

T

T

G

G

C

C

L

L

G

G

F

L

A

A

A

A

A

L

R

R

A

N

M

L

Q

K

S

Q

E

Q

L

F

K

R

G

G

Q

Q

R

R

V

V

G

G

V

I

L

I

I

V

S

T

G

G

G

G

N

N

V

V

D

D

L

I

A

E

R

K

F

Y

C

A

A

S

L

L

V

L

K53 (= K58) mutation to A: Loss of enzymatic activity.

Q7XSN8 Serine racemase; D-serine dehydratase; D-serine ammonia-lyase; L-serine dehydratase; L-serine ammonia-lyase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Oryza sativa subsp. japonica (Rice) (see paper)
42% identity, 92% coverage: 17:315/324 of query aligns to 27:329/339 of Q7XSN8

query
sites
Q7XSN8

A

A

A

Q

G

A

R

R

I

I

A

A

G

P

V

Y

A

V

H

H

R

K

T

T

P

P

V

V

L

L

T

S

S

S

R

T

T

S

V

I

N

D

E

A

E

I

F

V

G

G

A

K

Q

Q

V

L

F

F

F

F

K

K

C

C

E

E

N

C

L

F

Q

Q

R

K

M

A

G

G

A

A

F

F

K

K

F

I

R

R

G

G

A

A

Y

S

N

N

A

S

L

I

A

F

Q

A

F

L

S

D

P

D

A

D

Q

E

R

A

K

S

A

K

G

G

V

V

V

V

A

T

F

H

S

S

S

S

G

G

N

N

H

H

A

A

Q

A

G

A

I

V

A

A

L

L

A

A

A

A

R

K

E

L

L

R

G

G

I

I

P

P

A

A

T

Y

I

I

L

V

M

I

P

P

Q

R

D

N

A

A

P

P

A

A

A

C

K

K

V

V

A

D

A

N

T

V

Q

K

G

R

Y

Y

G

G

A

G

Q

H

V

I

V

I

F

W

F

S

D

D

R

V

Y

S

T

I

Q

E

D

S

R

R

E

E

Q

S

L

V

T

A

R

K

D

R

L

V

A

Q

E

E

R

E

H

T

G

G

L

A

T

I

L

L

I

V

P

H

P

P

Y

F

D

N

H

N

A

K

D

N

V

T

L

I

A

S

G

G

Q

Q

G

G

T

T

A

V

A

S

K

L

E

E

L

L

F

L

E

E

E

E

V

V

G

P

P

E

L

I

D

D

A

T

F

I

F

I

V

V

C

P

L

I

G

S

G

G

G

G

G

G

L

L

L

I

S

S

G

G

S

V

A

A

L

L

A

A

T

A

R

K

A

A

L

I

S

N

P

P

Q

S

T

I

K

R

L

I

Y

L

G

A

V

A

E
|
E

P

P

A

K

A

G

G

A

N

D

D
|
D

G

S

Q

A

Q

Q

S

S

F

K

R

A

S

A

G

G

S

K

I

I

V

I

H

T

I

L

D

P

T

S

P

T

T

N

T

T

I

I

A

A

D

D

G

G

A

L

Q

R

T

A

Q

-

H

F

L

L

G

G

H

D

I

L

T

T

F

W

A

P

I

V

I

V

R

R

R

D

D

L

V

V

D

D

D

D

I

I

L

I

T

V

V

V

T

D

D

D

E

N

Q

A

L

I

V

V

D

D

A

A

M

M

R

K

F

M

F

C

A

Y

E

E

R

M

M

L

K

K

L

V

V

A

V

V

E

E

P

P

T

S

G

G

C

A

L

I

G

G

F

L

A

A

A

A

A

-

R

-

A

A

M

L

Q

S

S

D

E

E

L

F

K

K

-

Q

-

S

-

S

-

A

-

W

-

H

-

E

G

S

Q

S

R

K

V

I

G

G

V

I

L

I

I

V

S

S

G

G

G

G

N

N

V

V

D

D

L

L

E219 (= E209) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-225.
D225 (= D215) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-219.

7nbfAAA structure of human serine racemase in complex with DSiP fragment Z126932614, XChem fragment screen (see paper)
40% identity, 97% coverage: 10:322/324 of query aligns to 5:323/323 of 7nbfAAA

query
sites
7nbfAAA

T

S

Y

F

D

A

D

D

V

V

V

E

A

K

A

A

A

H

G

I

R

N

I

I

A

R

G

D

V

S

A

I

H
|
H

R
x
L

T
|
T

P
|
P

V

V

L
|
L

T
|
T

S

S

R

S

T

I

V

L

N

N

E

Q

E

L

F

T

G

G

A

R

Q

N

V

L

F

F

F

F

K

K

C

C

E

E

N

L

L
x
F

Q

Q

R

K

M

T

G

G

A

S

F
|
F

K
|
K

F

I

R

R

G

G

A

A

Y

L

N

N

A

A

L

V

A

R

Q

S

F

L

S

V

P

P

-

D

-

A

-

L

A

E

Q

R

R

K

K

P

A

K

G

A

V

V

V

V

A

T

F

H

S

S

S
|
S

G

G

N
|
N

H

H

A

G

Q

Q

G

A

I

L

A

T

L

Y

A

A

A

A

R

K

E

L

L

E

G

G

I

I

P

P

A

A

T

Y

I

I

L

V

M

V

P

P

Q

Q

D

T

A

A

P

P

A

D

A

C

K

K

V

K

A

L

A

A

T

I

Q

Q

G

A

Y

Y

G

G

A

A

Q

S

V

I

V

V

F

Y

F

C

D

E

R

P

Y

S

T

D

Q

E

D

S

R

R

E

E

Q

N

L

V

T

A

R

K

D

R

L

V

A

T

E

E

R

E

H

T

G

E

L

G

T

I

L

M

I

V

P

H

P

P

Y

N

D

Q

H

E

A

P

D

A

V

V

L

I

A

A

G

G

Q

Q

G

G

T

T

A

I

A

A

K

L

E

E

L

V

F

L

E

N

E

Q

V

V

G

P

P

L

L

V

D

D

A

A

F

L

F

V

V

V

C

P

L

V

G
|
G

G
|
G

G
|
G

G
|
G

L
x
M

L

L

S

A

G

G

S

I

A

A

L

I

A

T

T

V

R

K

A

A

L

L

S

K

P

P

Q

S

T

V

K

K

L

V

Y

Y

G

A

V

A

E
|
E

P

P

A

S

A

N

G
x
A

N

D

D
|
D

G

C

Q

Y

Q

Q

S

S

F

K

R

L

S

K

G

G

S

K

I

L

V

M

-

P

H

N

I

L

D

Y

T

P

P

P

T

E

T

T

I

I

A

A

D

D

G
|
G

A
x
V

Q

K

T

S

Q

S

H

-

L

I

G

G

H

L

I
x
N

T

T

F

W

A

P

I

I

I

I

R

R

R

D

D

L

V

V

D

D

D

D

I

I

L

F

T

T

V

V

T

T

D

E

E

D

Q

E

L

I

V

K

D

C

A

A

M

T

R

Q

F

L

F

V

A

W

E

E

R

R

M

M

K

K

L

L

V

L

V

I

E

E

P

P

T
|
T

G

A

C

G

L

V

G

G

F

V

A

A

A

A

A

V

-

L

-

S

-

Q

-

H

-

F

R

Q

A

T

M

V

Q

S

S

P

E

E

L

V

K

K

G

-

Q

-

R

N

V

I

G

C

V

I

L

V

I
x
L

S
|
S

G
|
G

G

G

N

N

V

V

D

D

L

L

A

T

R

S

F

S

C

I

A

T

L

W

V

V

active site: K53 (= K58), S81 (= S83), E207 (= E209), A211 (≠ G213), D213 (= D215), G236 (= G237), L309 (≠ I308), S310 (= S309)
binding calcium ion: E207 (= E209), A211 (≠ G213), D213 (= D215)
binding magnesium ion: N244 (≠ I246)
binding pyridoxal-5'-phosphate: F52 (= F57), K53 (= K58), N83 (= N85), G182 (= G184), G183 (= G185), G184 (= G186), G185 (= G187), M186 (≠ L188), G236 (= G237), V237 (≠ A238), T282 (= T284), S310 (= S309), G311 (= G310)
binding 2-[(methylsulfonyl)methyl]-1H-benzimidazole: H21 (= H26), L22 (≠ R27), T23 (= T28), P24 (= P29), L26 (= L31), T27 (= T32), F46 (≠ L51)

Sites not aligning to the query:

binding magnesium ion: 3

7nbdAAA structure of human serine racemase in complex with DSiP fragment Z235449082, XChem fragment screen (see paper)
40% identity, 97% coverage: 10:322/324 of query aligns to 5:323/323 of 7nbdAAA

query
sites
7nbdAAA

T

S

Y

F

D

A

D

D

V

V

V

E

A

K

A

A

A

H

G

I

R

N

I

I

A

R

G

D

V

S

A

I

H

H

R

L

T

T

P

P

V

V

L

L

T

T

S

S

R

S

T

I

V

L

N

N

E

Q

E

L

F

T

G

G

A

R

Q

N

V

L

F

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R

K

M

T

G

G

A

S

F
|
F

K
|
K

F

I

R

R

G

G

A

A

Y

L

N

N

A

A

L

V

A

R

Q

S

F

L

S

V

P

P

-

D

-

A

-

L

A

E

Q

R

R

K

K

P

A

K

G

A

V

V

V

V

A

T

F

H

S

S

S
|
S

G

G

N
|
N

H

H

A

G

Q

Q

G

A

I

L

A

T

L

Y

A

A

A

A

R

K

E

L

L

E

G

G

I

I

P

P

A

A

T

Y

I

I

L

V

M

V

P

P

Q

Q

D

T

A

A

P

P

A

D

A

C

K

K

V

K

A

L

A

A

T

I

Q

Q

G

A

Y

Y

G

G

A

A

Q

S

V

I

V

V

F

Y

F

C

D

E

R

P

Y

S

T

D

Q

E

D

S

R

R

E

E

Q

N

L

V

T

A

R

K

D

R

L

V

A

T

E

E

R

E

H

T

G

E

L

G

T

I

L

M

I

V

P

H

P

P

Y

N

D

Q

H

E

A

P

D

A

V

V

L

I

A

A

G

G

Q

Q

G

G

T

T

A

I

A

A

K

L

E

E

L

V

F

L

E

N

E

Q

V

V

G

P

P

L

L

V

D

D

A

A

F

L

F

V

V

V

C

P

L

V

G
|
G

G
|
G

G
|
G

G
|
G

L
x
M

L

L

S

A

G

G

S

I

A

A

L

I

A

T

T

V

R

K

A

A

L

L

S

K

P

P

Q

S

T

V

K

K

L

V

Y

Y

G

A

V

A

E
|
E

P

P

A

S

A

N

G
x
A

N

D

D
|
D

G

C

Q

Y

Q

Q

S

S

F

K

R

L

S

K

G

G

S

K

I

L

V

M

-

P

H

N

I

L

D

Y

T

P

P

P

T

E

T

T

I

I

A

A

D

D

G
|
G

A
x
V

Q

K

T

S

Q

S

H

-

L

I

G

G

H

L

I
x
N

T

T

F

W

A

P

I

I

I

I

R

R

R

D

D

L

V

V

D

D

D

D

I

I

L

F

T

T

V

V

T

T

D

E

E

D

Q

E

L

I

V

K

D

C

A

A

M

T

R

Q

F

L

F

V

A
x
W

E

E

R

R

M

M

K

K

L

L

V
x
L

V

I

E
|
E

P

P

T
|
T

G

A

C

G

L

V

G

G

F

V

A

A

A

A

A

V

-

L

-

S

-

Q

-

H

-

F

R

Q

A

T

M

V

Q

S

S

P

E

E

L

V

K

K

G

-

Q

-

R

N

V

I

G

C

V

I

L

V

I
x
L

S
|
S

G
|
G

G

G

N

N

V
|
V

D

D

L
|
L

A

T

R

S

F

S

C

I

A

T

L

W

V

V

active site: K53 (= K58), S81 (= S83), E207 (= E209), A211 (≠ G213), D213 (= D215), G236 (= G237), L309 (≠ I308), S310 (= S309)
binding calcium ion: E207 (= E209), A211 (≠ G213), D213 (= D215)
binding [4-(1H-benzimidazol-1-yl)phenyl]methanol: W272 (≠ A274), L278 (≠ V280), V314 (= V313), L316 (= L315)
binding magnesium ion: N244 (≠ I246)
binding pyridoxal-5'-phosphate: F52 (= F57), K53 (= K58), N83 (= N85), G182 (= G184), G183 (= G185), G184 (= G186), G185 (= G187), M186 (≠ L188), G236 (= G237), V237 (≠ A238), E280 (= E282), T282 (= T284), S310 (= S309), G311 (= G310)

Sites not aligning to the query:

binding magnesium ion: 3

7nbcCCC structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
40% identity, 97% coverage: 10:322/324 of query aligns to 5:323/323 of 7nbcCCC

query
sites
7nbcCCC

T

S

Y

F

D

A

D

D

V

V

V

E

A

K

A

A

A

H

G

I

R

N

I

I

A

R

G

D

V

S

A

I

H

H

R

L

T

T

P

P

V

V

L

L

T

T

S

S

R

S

T

I

V

L

N

N

E

Q

E

L

F

T

G

G

A

R

Q

N

V

L

F

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R

K

M

T

G

G

A

S

F
|
F

K
|
K

F

I

R

R

G

G

A

A

Y

L

N

N

A

A

L

V

A

R

Q

S

F

L

S

V

P

P

-

D

-

A

-

L

A

E

Q

R

R

K

K

P

A

K

G

A

V

V

V

V

A
x
T

F
x
H

S

S

S
|
S

G

G

N
|
N

H
|
H

A

G

Q

Q

G

A

I

L

A

T

L

Y

A

A

A

A

R

K

E

L

L

E

G

G

I

I

P

P

A

A

T

Y

I

I

L

V

M

V

P

P

Q

Q

D

T

A

A

P

P

A

D

A

C

K

K

V

K

A

L

A

A

T

I

Q

Q

G

A

Y

Y

G

G

A

A

Q

S

V

I

V

V

F

Y

F

C

D

E

R

P

Y

S

T

D

Q

E

D

S

R

R

E

E

Q

N

L

V

T

A

R

K

D

R

L

V

A

T

E

E

R

E

H

T

G

E

L

G

T

I

L

M

I
x
V

P
x
H

P
|
P

Y

N

D

Q

H

E

A

P

D

A

V

V

L

I

A

A

G

G

Q

Q

G

G

T

T

A

I

A

A

K

L

E

E

L

V

F

L

E

N

E

Q

V

V

G

P

P

L

L

V

D

D

A

A

F

L

F

V

V

V

C

P

L

V

G
|
G

G
|
G

G
|
G

G
|
G

L
x
M

L

L

S

A

G

G

S

I

A

A

L

I

A

T

T

V

R

K

A

A

L

L

S

K

P

P

Q

S

T

V

K

K

L

V

Y

Y

G

A

V

A

E
|
E

P

P

A

S

A

N

G
x
A

N

D

D
|
D

G

C

Q

Y

Q

Q

S

S

F

K

R

L

S

K

G

G

S

K

I

L

V

M

-

P

H

N

I

L

D

Y

T

P

P

P

T

E

T

T

I

I

A

A

D

D

G
|
G

A
x
V

Q

K

T

S

Q

S

H

-

L

I

G

G

H

L

I

N

T

T

F

W

A

P

I

I

I

I

R

R

R

D

D

L

V

V

D

D

D

D

I

I

L

F

T

T

V

V

T

T

D

E

E

D

Q

E

L

I

V

K

D

C

A

A

M

T

R

Q

F

L

F

V

A

W

E

E

R

R

M

M

K

K

L

L

V

L

V

I

E

E

P

P

T
|
T

G

A

C

G

L

V

G

G

F

V

A

A

A

A

A

V

-

L

-

S

-

Q

-

H

-

F

R

Q

A

T

M

V

Q

S

S

P

E

E

L

V

K

K

G

-

Q

-

R

N

V

I

G

C

V

I

L

V

I
x
L

S
|
S

G
|
G

G

G

N

N

V

V

D

D

L

L

A

T

R

S

F

S

C

I

A

T

L

W

V

V

active site: K53 (= K58), S81 (= S83), E207 (= E209), A211 (≠ G213), D213 (= D215), G236 (= G237), L309 (≠ I308), S310 (= S309)
binding biphenyl-4-ylacetic acid: T78 (≠ A80), H79 (≠ F81), H84 (= H86), V148 (≠ I150), H149 (≠ P151), P150 (= P152)
binding calcium ion: E207 (= E209), A211 (≠ G213), D213 (= D215)
binding pyridoxal-5'-phosphate: F52 (= F57), K53 (= K58), N83 (= N85), G182 (= G184), G183 (= G185), G184 (= G186), G185 (= G187), M186 (≠ L188), G236 (= G237), V237 (≠ A238), T282 (= T284), S310 (= S309), G311 (= G310)

7nbcAAA structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
40% identity, 97% coverage: 10:322/324 of query aligns to 5:323/323 of 7nbcAAA

query
sites
7nbcAAA

T

S

Y

F

D

A

D

D

V

V

V

E

A

K

A

A

A

H

G

I

R

N

I

I

A

R

G

D

V

S

A

I

H

H

R

L

T

T

P

P

V

V

L

L

T

T

S

S

R

S

T

I

V

L

N

N

E

Q

E

L

F

T

G

G

A

R

Q

N

V

L

F

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R

K

M

T

G

G

A

S

F
|
F

K
|
K

F

I

R

R

G

G

A

A

Y

L

N

N

A

A

L

V

A

R

Q

S

F

L

S

V

P

P

-

D

-

A

-

L

A

E

Q

R

R

K

K

P

A

K

G

A

V

V

V

V

A

T

F

H

S

S

S
|
S

G

G

N
|
N

H

H

A

G

Q

Q

G

A

I

L

A

T

L

Y

A

A

A

A

R

K

E

L

L

E

G

G

I

I

P

P

A

A

T

Y

I

I

L

V

M

V

P

P

Q

Q

D

T

A

A

P

P

A

D

A

C

K

K

V

K

A

L

A

A

T

I

Q

Q

G

A

Y

Y

G

G

A

A

Q

S

V

I

V

V

F

Y

F

C

D

E

R

P

Y

S

T

D

Q

E

D

S

R

R

E

E

Q

N

L

V

T

A

R

K

D

R

L

V

A

T

E

E

R

E

H

T

G

E

L

G

T

I

L

M

I

V

P

H

P

P

Y

N

D

Q

H

E

A

P

D

A

V

V

L

I

A

A

G

G

Q

Q

G

G

T

T

A

I

A

A

K

L

E

E

L

V

F

L

E

N

E

Q

V

V

G

P

P

L

L

V

D

D

A

A

F

L

F

V

V

V

C

P

L

V

G
|
G

G
|
G

G
|
G

G
|
G

L
x
M

L

L

S

A

G

G

S

I

A

A

L

I

A

T

T

V

R

K

A

A

L

L

S

K

P

P

Q

S

T

V

K

K

L

V

Y

Y

G

A

V

A

E
|
E

P

P

A

S

A

N

G
x
A

N

D

D
|
D

G

C

Q

Y

Q

Q

S

S

F

K

R

L

S

K

G

G

S

K

I

L

V

M

-

P

H

N

I

L

D

Y

T

P

P

P

T

E

T

T

I

I

A

A

D

D

G
|
G

A
x
V

Q

K

T

S

Q

S

H

-

L

I

G

G

H

L

I
x
N

T

T

F

W

A

P

I

I

I

I

R

R

R

D

D

L

V

V

D

D

D

D

I

I

L

F

T

T

V

V

T

T

D

E

E

D

Q

E

L

I

V

K

D

C

A

A

M

T

R

Q

F

L

F

V

A

W

E

E

R

R

M

M

K

K

L

L

V

L

V

I

E

E

P

P

T
|
T

G

A

C

G

L

V

G

G

F

V

A

A

A

A

A

V

-

L

-

S

-

Q

-

H

-

F

R

Q

A

T

M

V

Q

S

S

P

E

E

L

V

K

K

G

-

Q

-

R

N

V

I

G

C

V

I

L

V

I
x
L

S
|
S

G
|
G

G

G

N

N

V

V

D

D

L

L

A

T

R

S

F

S

C

I

A

T

L

W

V

V

active site: K53 (= K58), S81 (= S83), E207 (= E209), A211 (≠ G213), D213 (= D215), G236 (= G237), L309 (≠ I308), S310 (= S309)
binding calcium ion: E207 (= E209), A211 (≠ G213), D213 (= D215)
binding magnesium ion: N244 (≠ I246)
binding pyridoxal-5'-phosphate: F52 (= F57), K53 (= K58), N83 (= N85), G182 (= G184), G183 (= G185), G184 (= G186), G185 (= G187), M186 (≠ L188), G236 (= G237), V237 (≠ A238), T282 (= T284), S310 (= S309), G311 (= G310)

Sites not aligning to the query:

binding magnesium ion: 3

7nbhAAA structure of human serine racemase in complex with DSiP fragment Z26781964, XChem fragment screen (see paper)
40% identity, 94% coverage: 10:315/324 of query aligns to 5:316/320 of 7nbhAAA

query
sites
7nbhAAA

T

S

Y

F

D

A

D

D

V

V

V

E

A

K

A

A

A

H

G

I

R

N

I

I

A

R

G

D

V

S

A

I

H

H

R

L

T

T

P

P

V

V

L

L

T

T

S

S

R

S

T

I

V

L

N

N

E

Q

E

L

F

T

G

G

A

R

Q

N

V

L

F

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R

K

M

T

G

G

A

S

F

F

K
|
K

F

I

R

R

G

G

A

A

Y

L

N

N

A

A

L

V

A

R

Q

S

F

L

S

V

P

P

-

D

-

A

-

L

A

E

Q

R

R

K

K

P

A

K

G

A

V

V

V

V

A

T

F

H

S

S

S
|
S

G

G

N

N

H

H

A
x
G

Q
|
Q

G

A

I

L

A

T

L

Y

A

A

A

A

R

K

E

L

L

E

G

G

I

I

P

P

A

A

T

Y

I

I

L

V

M

V

P

P

Q

Q

D

T

A

A

P

P

A

D

A

C

K
|
K

V

K

A

L

A

A

T
x
I

Q

Q

G

A

Y
|
Y

G

G

A

A

Q

S

V

I

V

V

F

Y

F

C

D

E

R

P

Y

S

T

D

Q

E

D

S

R

R

E

E

Q

N

L

V

T

A

R

K

D

R

L

V

A

T

E

E

R

E

H

T

G

E

L

G

T

I

L

M

I

V

P

H

P

P

Y

N

D

Q

H

E

A

P

D

A

V

V

L

I

A

A

G

G

Q

Q

G

G

T

T

A

I

A

A

K

L

E

E

L

V

F

L

E

N

E

Q

V

V

G

P

P

L

L

V

D

D

A

A

F

L

F

V

V

V

C

P

L

V

G

G

G

G

G

G

G

G

L

M

L

L

S

A

G

G

S

I

A

A

L

I

A

T

T

V

R

K

A

A

L

L

S

K

P

P

Q

S

T

V

K

K

L

V

Y

Y

G

A

V

A

E
|
E

P

P

A

S

A

N

G
x
A

N

D

D
|
D

G

C

Q

Y

Q

Q

S

S

F

K

R

L

S

K

G

G

S

K

I

L

V

M

-

P

H

N

I

L

D

Y

T

P

P

P

T

E

T

T

I

I

A

A

D
|
D

G
|
G

A

V

Q

K

T

S

Q

S

H

-

L

I

G

G

H

L

I

N

T

T

F

W

A

P

I

I

I

I

R

R

R

D

D

L

V

V

D

D

D

D

I

I

L

F

T

T

V

V

T

T

D

E

E

D

Q

E

L

I

V

K

D

C

A

A

M

T

R

Q

F

L

F

V

A

W

E

E

R

R

M

M

K

K

L

L

V

L

V

I

E

E

P
|
P

T

T

G

A

C

G

L

V

G

G

F

V

A

A

A

A

A

V

-

L

-

S

-

Q

-

H

-

F

R

Q

A

T

M

V

Q

S

S

P

E

E

L

V

K

K

G

-

Q

-

R

N

V

I

G

C

V

I

L

V

I
x
L

S
|
S

G

G

G

G

N
|
N

V
|
V

D
|
D

L

L

active site: K53 (= K58), S81 (= S83), E207 (= E209), A211 (≠ G213), D213 (= D215), G236 (= G237), L309 (≠ I308), S310 (= S309)
binding calcium ion: E207 (= E209), A211 (≠ G213), D213 (= D215)
binding N-[(1H-benzimidazol-2-yl)methyl]furan-2-carboxamide: S81 (= S83), G85 (≠ A87), Q86 (= Q88), K111 (= K113), I115 (≠ T117), Y118 (= Y120), D235 (= D236), P281 (= P283), N313 (= N312), V314 (= V313), D315 (= D314)

7nbgAAA structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
40% identity, 94% coverage: 10:315/324 of query aligns to 5:316/322 of 7nbgAAA

query
sites
7nbgAAA

T

S

Y

F

D

A

D

D

V

V

V

E

A

K

A

A

A

H

G

I

R

N

I

I

A

R

G

D

V

S

A

I

H

H

R

L

T

T

P

P

V

V

L

L

T

T

S

S

R

S

T

I

V

L

N

N

E

Q

E

L

F

T

G

G

A

R

Q

N

V

L

F

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R

K

M

T

G

G

A

S

F
|
F

K
|
K

F

I

R

R

G

G

A

A

Y

L

N

N

A

A

L

V

A

R

Q

S

F

L

S

V

P

P

-

D

-

A

-

L

A

E

Q

R

R

K

K

P

A

K

G

A

V

V

V

V

A

T

F

H

S

S

S
|
S

G

G

N
|
N

H

H

A
x
G

Q
|
Q

G

A

I

L

A

T

L

Y

A

A

A

A

R

K

E

L

L

E

G

G

I

I

P

P

A

A

T

Y

I
|
I

L

V

M

V

P

P

Q

Q

D

T

A

A

P

P

A

D

A

C

K
|
K

V

K

A

L

A

A

T
x
I

Q

Q

G

A

Y
|
Y

G

G

A

A

Q

S

V

I

V

V

F

Y

F

C

D

E

R

P

Y

S

T

D

Q

E

D

S

R

R

E

E

Q

N

L

V

T

A

R

K

D

R

L

V

A

T

E

E

R

E

H

T

G

E

L

G

T

I

L

M

I

V

P

H

P

P

Y

N

D

Q

H

E

A

P

D

A

V

V

L

I

A

A

G

G

Q

Q

G

G

T

T

A

I

A

A

K

L

E

E

L

V

F

L

E

N

E

Q

V

V

G

P

P

L

L

V

D

D

A

A

F

L

F

V

V

V

C

P

L

V

G
|
G

G
|
G

G
|
G

G
|
G

L
x
M

L

L

S

A

G

G

S

I

A

A

L

I

A

T

T

V

R

K

A

A

L

L

S

K

P

P

Q

S

T

V

K

K

L

V

Y

Y

G

A

V

A

E
|
E

P

P

A

S

A

N

G
x
A

N

D

D
|
D

G

C

Q

Y

Q

Q

S

S

F

K

R

L

S

K

G

G

S

K

I

L

V

M

-

P

H

N

I

L

D

Y

T

P

P

P

T

E

T

T

I

I

A

A

D

D

G
|
G

A
x
V

Q

K

T

S

Q

S

H

-

L

I

G

G

H

L

I

N

T

T

F

W

A

P

I

I

I

I

R

R

R

D

D

L

V

V

D

D

D

D

I

I

L

F

T

T

V

V

T

T

D

E

E

D

Q

E

L

I

V

K

D

C

A

A

M

T

R

Q

F

L

F

V

A

W

E

E

R

R

M

M

K

K

L

L

V

L

V

I

E

E

P

P

T
|
T

G

A

C

G

L

V

G

G

F

V

A

A

A

A

A

V

-

L

-

S

-

Q

-

H

-

F

R

Q

A

T

M

V

Q

S

S

P

E

E

L

V

K

K

G

-

Q

-

R

N

V

I

G

C

V

I

L

V

I
x
L

S
|
S

G
|
G

G

G

N

N

V

V

D

D

L

L

active site: K53 (= K58), S81 (= S83), E207 (= E209), A211 (≠ G213), D213 (= D215), G236 (= G237), L309 (≠ I308), S310 (= S309)
binding calcium ion: E207 (= E209), A211 (≠ G213), D213 (= D215)
binding pyridoxal-5'-phosphate: F52 (= F57), K53 (= K58), N83 (= N85), G182 (= G184), G183 (= G185), G184 (= G186), G185 (= G187), M186 (≠ L188), G236 (= G237), V237 (≠ A238), T282 (= T284), S310 (= S309), G311 (= G310)
binding ~{N}-[2-(2-methylphenyl)ethyl]ethanamide: S81 (= S83), G85 (≠ A87), Q86 (= Q88), I101 (= I103), K111 (= K113), I115 (≠ T117), Y118 (= Y120)

6zspAAA serine racemase bound to atp and malonate. (see paper)
40% identity, 97% coverage: 10:324/324 of query aligns to 5:318/320 of 6zspAAA

query
sites
6zspAAA

T

S

Y

F

D

A

D

D

V

V

V

E

A

K

A

A

A

H

G

I

R

N

I

I

A

R

G

D

V

S

A

I

H

H

R

L

T

T

P

P

V

V

L