SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Ac3H11_3809 FitnessBrowser__acidovorax_3H11:Ac3H11_3809 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7vw6B Cryo-em structure of formate dehydrogenase 1 from methylorubrum extorquens am1 (see paper)
57% identity, 90% coverage: 32:605/635 of query aligns to 21:555/560 of 7vw6B

query
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7vw6B

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binding fe2/s2 (inorganic) cluster: C108 (= C122), S110 (= S124), T112 (≠ S126), C113 (= C127), C141 (= C158), V142 (= V159), G143 (= G160), L144 (≠ R161), C145 (= C162)
binding flavin mononucleotide: G221 (= G267), G223 (= G269), K232 (= K278), N248 (= N294), D250 (= D296), E251 (= E297), Y324 (= Y374), G327 (= G377), E328 (= E378), E329 (= E379), I362 (≠ E412), N363 (≠ H413), N364 (= N414), T367 (= T417), G538 (= G588), L539 (= L589)
binding iron/sulfur cluster: I325 (= I375), P343 (= P393), S496 (= S546), C497 (= C547), G498 (= G548), Q499 (= Q549), C500 (= C550), C503 (= C553), I536 (= I586), C537 (= C587), L539 (= L589), G540 (= G590)

7q5yC Structure of nadh:ubichinon oxidoreductase (complex i) of the hyperthermophilic eubacterium aquifex aeolicus
40% identity, 61% coverage: 222:607/635 of query aligns to 22:413/419 of 7q5yC

query
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7q5yC

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binding flavin mononucleotide: G65 (= G267), R66 (≠ L268), G67 (= G269), K76 (= K278), N92 (= N294), D94 (= D296), E95 (= E297), Y180 (= Y374), G183 (= G377), E184 (= E378), E185 (= E379), V218 (≠ E412), N219 (≠ H413), N220 (= N414), T223 (= T417), G394 (= G588), L395 (= L589)
binding iron/sulfur cluster: I181 (= I375), P199 (= P393), T346 (≠ S546), C347 (= C547), G348 (= G548), Q349 (= Q549), C350 (= C550), C353 (= C553), S391 (= S585), I392 (= I586), C393 (= C587), L395 (= L589), G396 (= G590)

6q9cB Crystal structure of aquifex aeolicus nadh-quinone oxidoreductase subunits nuoe and nuof bound to nadh under anaerobic conditions (see paper)
40% identity, 61% coverage: 222:607/635 of query aligns to 21:412/418 of 6q9cB

query
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6q9cB

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G

V

V

K

Y

L

E

A

L

P

P

A

M

G

N

I

T

T

T

V

L

Q

R

E

E

L

V

I

I

D

F

E

K

Y

Y

C

A

G

G

L

T

L

L

D

G

G

N

H

K

Q

K

L

V

Y

K

A

A

Y

V

L

F

P

S

G

G

G

A

A
x
L

S

D

G

-

I

C

L

F

P

S

A

S

S

E

L

E

N

L

Q

D

I

I

P

P

L

M

D

D

F

Y

D

S

T

P

L

L

Q

G

P

-

Y

-

G

-

C

-

F

F

I

G

G

G

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x
T

A

G

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T

V

V

I

I

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V

L

L

S

T

Q

E

H

E

D

D

R

D

A

I

R

V

D

E

A

A

L

L

N

K

V

I

M

A

R

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F

F

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E

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H

E

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x
T

C
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C

G
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G

Q
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Q

C
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C

T

T

P

P

C
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C

R

R

V

V

G

G

T

C

A

Y

K

E

A

Q

A

A

T

N

L

L

M

L

-

E

-

K

-

I

-

Y

-

K

-

G

E

E

A

A

P

T

Q

E

W

Q

D

D

G

W

D

E

L

G

L

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D

D

D

F

L

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A

N

Q

R

V

N

M

I

G

Q

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A

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S
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S

I
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I

C
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C

G
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G

L
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L

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G

Q

A

A

V

A

A

P

G

N

R

P

L

I

I

R

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C

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L

K

E

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K

F

F

P

P

H

E

E

E

binding flavin mononucleotide: G64 (= G267), R65 (≠ L268), G66 (= G269), K75 (= K278), N91 (= N294), D93 (= D296), E94 (= E297), Y179 (= Y374), G182 (= G377), E183 (= E378), E184 (= E379), V217 (≠ E412), N218 (≠ H413), N219 (= N414), T222 (= T417), G393 (= G588), L394 (= L589)
binding 1,4-dihydronicotinamide adenine dinucleotide: G66 (= G269), G67 (= G270), A68 (= A271), F70 (= F273), K75 (= K278), E94 (= E297), S95 (≠ G298), E96 (= E299), Y179 (= Y374), E184 (= E379), Y204 (= Y399), V206 (≠ A401), L296 (≠ A491), T318 (≠ S519)
binding iron/sulfur cluster: I180 (= I375), P198 (= P393), T345 (≠ S546), C346 (= C547), G347 (= G548), Q348 (= Q549), C349 (= C550), C352 (= C553), S390 (= S585), I391 (= I586), C392 (= C587), L394 (= L589), G395 (= G590)

6saqB Wild-type nuoef from aquifex aeolicus bound to nadh-oh (see paper)
40% identity, 61% coverage: 222:607/635 of query aligns to 21:412/419 of 6saqB

query
sites
6saqB

R

R

E

A

V

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S

K

P

P

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Y

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G

Y

Y

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A

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A

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A

K

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A

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x
F

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N

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R

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N

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E

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E

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P

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T

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D

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I

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F

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G

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G

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L

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Y

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x
V

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G

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L

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P

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T

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x
N

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N

F

V

E

E

T
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T

L

I

Y

A

W

N

V

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x
R

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I

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F

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K

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K

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G

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A

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Y

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G
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T

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P

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C

R

R

V

V

G

G

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C

A

Y

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E

A

Q

A

A

T

N

L

L

M

L

-

E

-

K

-

I

-

Y

-

K

-

G

E

E

A

A

P

T

Q

E

W

Q

D

D

G

W

D

E

L

G

L

F

D

D

D

F

L

V

A

N

Q

R

V

N

M

I

G

Q

D

P

A

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S
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S

I
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I

C
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C

G
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G

L
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L

G
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Q

A

A

V

A

A

P

G

N

R

P

L

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L

K

E

Y

K

F

F

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H

E

E

E

binding flavin mononucleotide: G64 (= G267), R65 (≠ L268), G66 (= G269), K75 (= K278), N91 (= N294), D93 (= D296), E94 (= E297), Y179 (= Y374), G182 (= G377), E183 (= E378), E184 (= E379), V217 (≠ E412), N218 (≠ H413), N219 (= N414), T222 (= T417), G393 (= G588), L394 (= L589)
binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{S},4~{R},5~{R})-5-[[(1~{E},3~{Z})-5-azanyl-4-oxidanyl-5-oxidanylidene-penta-1,3-dienyl]-methanoyl-amino]-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate: G66 (= G269), G67 (= G270), A68 (= A271), F70 (= F273), K75 (= K278), F78 (≠ I281), E94 (= E297), E96 (= E299), T99 (= T302), D102 (= D305), Y179 (= Y374), E184 (= E379), K201 (≠ R396), Y204 (= Y399), V206 (≠ A401), N219 (= N414), W234 (≠ P429), R238 (≠ A433), L296 (≠ A491), T318 (≠ S519)
binding iron/sulfur cluster: I180 (= I375), P198 (= P393), T345 (≠ S546), C346 (= C547), G347 (= G548), Q348 (= Q549), C349 (= C550), C352 (= C553), S390 (= S585), I391 (= I586), C392 (= C587), L394 (= L589), G395 (= G590)

6hl3B Wild-type nuoef from aquifex aeolicus - oxidized form bound to NAD+ (see paper)
40% identity, 61% coverage: 222:607/635 of query aligns to 20:411/416 of 6hl3B

query
sites
6hl3B

R

R

E

A

V

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K

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D

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Y

Y

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G

Y

Y

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A

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-

M

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A

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A

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M

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R

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G

A
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A

G

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F

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G

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K
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W

W

R

K

I

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N

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P

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P

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R

L

Y

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A

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C

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N

I

A

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D

E
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E

G

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E

P

P

G

G

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F

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D

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I

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I

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N

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I

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Y

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G

E

E

Y

Y

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R

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A

A

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E

L

A

R

K

A

K

E

K

P

G

P

F

C

L

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G

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N

-

I

-

L

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G

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S

-

G

-

F

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D

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R

G

G

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A

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I

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G

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E

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N

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N

F

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T

L

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N

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P

C
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C

R

R

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V

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C

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N

L

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M

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D

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A

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E

binding flavin mononucleotide: G63 (= G267), R64 (≠ L268), G65 (= G269), K74 (= K278), N90 (= N294), D92 (= D296), E93 (= E297), Y178 (= Y374), G181 (= G377), E182 (= E378), E183 (= E379), V216 (≠ E412), N217 (≠ H413), N218 (= N414), T221 (= T417), G392 (= G588), L393 (= L589)
binding nicotinamide-adenine-dinucleotide: G65 (= G269), G66 (= G270), A67 (= A271), F69 (= F273), K74 (= K278), E93 (= E297), E95 (= E299), Y178 (= Y374), E183 (= E379), K200 (≠ R396), Y203 (= Y399), V205 (≠ A401), L295 (≠ A491)
binding iron/sulfur cluster: I179 (= I375), P197 (= P393), T344 (≠ S546), C345 (= C547), G346 (= G548), Q347 (= Q549), C348 (= C550), C351 (= C553), S389 (= S585), I390 (= I586), C391 (= C587), L393 (= L589), G394 (= G590)

8a6tB Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from thermoanaerobacter kivui in the reduced state (see paper)
34% identity, 77% coverage: 118:607/635 of query aligns to 27:554/630 of 8a6tB

query
sites
8a6tB

V

V

L

I

R

R

V

I

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C

D

G

S
x
G

L

T

S
x
G

C
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C

S

R

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A

A

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G

G

A

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R

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A

L

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L

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D

A

E

L

L

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V

E

K

R

V

L

L

R

K

A

R

A

E

G

G

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F

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-

V

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V

-

N

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L

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S

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D

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C

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L

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E

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N

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V

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V

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Q

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F

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C

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A

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Q

A

G

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V

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A

M

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V

I

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Q

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A

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G

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V

L

E

E

E

V

I

V

V

E

E

S

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K

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P

I

K

K

-

K

-

N

-

E

-

I

-

E

R

R

A

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L

L

A

Y

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P

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A

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A

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A

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L

L

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-

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P

A

A

H

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-

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-

L

V

A

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D

Y

Y

R

I

A

A

H

E

G

G

Y

Y

Q

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A

A

I

A

A

A

K

L

A

V

L

N

T

G

-

E

-

M

M

D

D

A

R

E

K

A

Q

V

I

L

I

A

D

A

E

M

V

E

I

D

K

S

S

G

G

L

L

R

R

G
|
G

L
x
R

G
|
G

G

G

A
|
A

G

G

F
|
F

P

P

A

T

G

G

R

E

K
|
K

W

W

R

L

-

G

I

A

V

Y

R

K

D

N

Q

Q

P

S

A

P

P

K

R

K

L

Y

M

I

A

I

V

C

N
|
N

I

G

D
|
D

E
|
E

G

G

E

D

P

P

G

G

T

A

F

F

K

M

D

D

R

R

T

S

Y

V

L

M

E

E

R

G

D

D

P

P

H

H

R

K

F

V

L

I

E

E

G

G

V

M

L

M

I

I

A

G

A

A

Q

Y

V

A

V

I

G

G

T

S

E

D

A

E

I

G

Y

Y

I

I

Y

Y

L

V

R

R

D

A

E

E

Y

Y

H

P

G

L

C

A

R

V

V

Q

L

M

L

L

Q

R

Q

K

A

A

L

I

-

E

-

C

-

E

-

K

-

L

-

G

-

L

-

G

D

D

D

N

L

I

R

L

A

G

E

T

P

G

P

F

C

S

P

F

L

R

P

L

H

H

I

V

E

-

L

-

R

R

R

E

G

G

A

A

G

G

A

A

Y
x
F

I

V

C

C

G
|
G

E
|
E

E
x
S

S

T

A

A

M

L

I

T

E

Y

S

S

I

I

E

E

G

G

K

K

R

R

G

G

E

M

P
|
P

R

R

M

V

R
|
R

P

P

Y
x
R

I
x
T

A
x
N

Q

E

V

C

G

G

L

L

F

W

G

E

R

M

P

P

T

T

L

V

E
x
L

H
x
N

N
|
N

F

V

E

E

T
|
T

L

F

Y

A

W

C

V

I

R

P

D

E

I

I

V

I

E

L

R

N

G

G

P

G

Q

E

W

W

F

F

A

A

S

S

F

I

G

G

R

T

H

P

G

T

R

S

K

T

G

G

L

T

R

K

S

I

F

F

S

A

V

L

S

S

G

G

R

K

V

V

K

N

H

R

P

T

G

G

V

L

K

V

L

E

A

V

P

P

A

M

G

G

I

L

T

K

V

L

Q

R

E

E

L

L

I

I

D

F

E

D

Y

I

C

G

G

G

L

I

L

A

D

N

G

N

H

K

Q

K

L

F

Y

K

A

A

Y

V

L

Q

P

L

G

G

A

P

S
|
S

G

G

I

C

L

V

P

P

A

E

S

S

L

Q

N

L

Q

D

I

L

P

P

L

I

D

D

F

F

D

D

T

S

L

L

Q

S

P

K

Y

A

G

G

C

A

F

I

I

M

G

G

S
|
S

A

G

V

V

I

V

V

V

L

V

S

D

Q

E

H

D

D

T

R
x
C

A

M

R

V

D

D

A

F

A

A

L

K

N

F

V

F

M

T

R

N

F

F

F

I

E

V

H

E

E

E

S
|
S

C
|
C

G
|
G

Q
x
K

C
|
C

T

I

P

P

C
|
C

R

R

V

E

G

G

T

N

A

K

K

K

A

M

A

L

T

E

L

I

M

L

E

E

-

R

-

I

-

T

-

E

-

G

-

K

A

G

P

K

Q

E

W

G

D

D

G

I

D

E

L

L

D

E

L

L

A

G

Q

D

V

V

M

I

G

I

D

S

A

A

S

S

I

L

C
|
C

G
|
G

L
|
L

G
|
G

Q

K

A

T

A

A

P

P

N

N

P

P

I

V

R

L

C

S

I

T

H

I

K

K

Y

H

F

F

P

R

H

D

E

E

binding fe2/s2 (inorganic) cluster: C31 (= C122), G33 (≠ S124), G35 (≠ S126), C36 (= C127), C82 (= C158), Q83 (≠ V159), G84 (= G160), F85 (≠ R161), C86 (= C162)
binding flavin mononucleotide: G199 (= G267), R200 (≠ L268), G201 (= G269), K210 (= K278), N227 (= N294), D229 (= D296), E230 (= E297), F315 (≠ Y374), G318 (= G377), E319 (= E378), L353 (≠ E412), N354 (≠ H413), N355 (= N414), T358 (= T417), G535 (= G588), L536 (= L589)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G201 (= G269), A203 (= A271), F205 (= F273), K210 (= K278), E319 (= E378), S320 (≠ E379), R337 (= R396), R340 (≠ Y399), T341 (≠ I400), N342 (≠ A401), S433 (= S492), S460 (= S519)
binding iron/sulfur cluster: P334 (= P393), S487 (= S546), C488 (= C547), G489 (= G548), K490 (≠ Q549), C491 (= C550), C494 (= C553), C534 (= C587), L536 (= L589), G537 (= G590)
binding zinc ion: C471 (≠ R530)

Sites not aligning to the query:

binding iron/sulfur cluster: 575, 577, 582, 583, 584, 585, 588, 588, 592, 596, 597, 605, 607, 612, 614, 615, 618, 622, 624, 626, 627
binding zinc ion: 558, 564, 569

7o6yB Cryo-em structure of respiratory complex i under turnover (see paper)
39% identity, 60% coverage: 230:608/635 of query aligns to 22:419/457 of 7o6yB

query
sites
7o6yB

D

D

L

L

A

A

T

S

Y

A

R

R

A

K

H

Q

G

G

G

D

-

W

Y

Y

Q

K

T

T

A

K

A

E

A

L

L

I

V

L

N

K

G

G

E

D

M

T

D

-

A

-

E

-

A

W

V

I

L

I

A

D

A

E

M

I

E

K

D

K

S

S

G

G

L

L

R

R

G
|
G

L

R

G
|
G

A
|
A

G

G

F
|
F

P

P

A

S

G

G

R

L

K
|
K

W

W

R

S

I
x
F

V

M

R

N

-

P

-

G

-

W

-

E

D

K

Q

N

P

E

A

G

P

P

R

R

L

Y

M

L

A

V

V

V

N
|
N

I

A

D
|
D

E
|
E

G

G

E
|
E

P

P

G

G

T

T

F

C

K

K

D

D

R

R

T

E

Y

I

L

M

E

R

R

K

D

D

P

P

H

H

R

K

F

L

L

V

E

E

G

G

V

C

L

L

I

L

A

A

A

G

Q

R

V

A

V

M

G

N

T

A

E

T

A

A

I

A

Y

Y

I

I

Y

Y

L

I

R

R

D

G

E

E

Y

F

H

Y

G

N

C

E

R

A

V

A

L

V

L

L

Q

Q

Q

T

A

A

L

I

D

N

D

E

-

A

-

Y

-

A

-

G

L

L

R

I

A

G

E

K

P

D

P

A

C

C

P

G

L

S

P

G

H

Y

-

D

-

F

-

D

I

V

E

Y

L

I

R

H

R

R

G

G

A

M

G

G

A

A

Y
|
Y

I

V

C

C

G
|
G

E
|
E

S

T

A

S

M

L

I

I

E

E

S

S

I

L

E

E

G

G

K

K

R

A

G

G

E

K

P
|
P

R

R

M

L

R

K

P

P

Y
x
F

I
x
P

A

A

Q

G

V

V

G

G

L

L

F

F

G

G

R

R

P

P

T

S

L

T

E
x
V

H
x
T

N
|
N

F

V

E

E

T

T

L

V

Y

A

W

V

V

A

R

P

D

T

I

I

V

L

E

R

R

R

G

G

P

G

Q

D

W

W

F

F

A

A

S

S

F

F

G

G

R

R

H

E

G

R

R

N

K

S

G

G

L

T

R

K

S

L

F

F

S

C

V

I

S

S

G

G

R

N

V

V

K

N

H

E

P

P

G

C

V

T

K

V

L

E

A

E

P

E

A

M

G

S

I

I

T

P

V

L

Q

R

E

E

L

L

I

L

D

E

E

K

Y

H

C

C

G

G

L

I

L

K

D

G

G

G

-

W

H

D

Q

N

L

L

Y

L

A

G

Y

V

L

I

P

P

G

G

A

C

S

S

G

V

G

P

I

I

L

L

P

P

A

K

S

N

L

I

-

C

N

E

Q

D

I

V

P

L

L

M

D

D

F

F

D

D

T

A

L

L

Q

K

P

D

Y

V

G

Q

C

S

F

G

I

L

G

G

S
x
T

A

A

V

V

I

I

V

V

L

I

S

N

Q

K

H

Q

D

Q

R

D

A

V

R

I

D

R

A

A

A

I

L

Q

N

R

V

F

M

A

R

A

F

F

F

Y

E

K

H

H

E

E

S
|
S

C
|
C

G
|
G

Q
|
Q

C
|
C

T

T

P

P

C
|
C

R

R

V

E

G

G

T

T

A

T

K

W

A

L

T

K

L

A

M

M

-

D

-

R

-

F

-

R

-

T

-

G

E

Q

A

A

P

K

Q

E

W

R

D

E

G

I

D

D

L

M

L

L

D

Y

D

E

L

L

A

T

Q

K

V

D

M

I

G

E

D

G

A

H

S
x
T

I

I

C
|
C

G
x
A

L
|
L

G

G

Q

D

A

A

P

A

N

W

P

P

I

I

R

Q

C

G

I

L

H

I

K

R

Y

N

F

F

P

R

H

P

E

E

V

M

binding flavin mononucleotide: G57 (= G267), G59 (= G269), G60 (= G270), A61 (= A271), K68 (= K278), N89 (= N294), D91 (= D296), E92 (= E297), Y177 (= Y374), G180 (= G377), E181 (= E378), E182 (= E379), V215 (≠ E412), T216 (≠ H413), N217 (= N414), A399 (≠ G588), L400 (= L589)
binding 1,4-dihydronicotinamide adenine dinucleotide: G59 (= G269), G60 (= G270), A61 (= A271), F63 (= F273), K68 (= K278), F71 (≠ I281), E94 (= E299), Y177 (= Y374), E181 (= E378), E182 (= E379), F202 (≠ Y399), P203 (≠ I400), T324 (≠ S519), A399 (≠ G588)
binding iron/sulfur cluster: P196 (= P393), S351 (= S546), C352 (= C547), G353 (= G548), Q354 (= Q549), C355 (= C550), C358 (= C553), T396 (≠ S585), C398 (= C587), L400 (= L589)

7b0nF 3.7-angstrom structure of Yarrowia lipolytica complex I with an R121M mutation in NUCM. (see paper)
39% identity, 60% coverage: 230:608/635 of query aligns to 23:420/460 of 7b0nF

query
sites
7b0nF

D

D

L

L

A

A

T

S

Y

A

R

R

A

K

H

Q

G

G

G

D

-

W

Y

Y

Q

K

T

T

A

K

A

E

A

L

L

I

V

L

N

K

G

G

E

D

M

T

D

-

A

-

E

-

A

W

V

I

L

I

A

D

A

E

M

I

E

K

D

K

S

S

G

G

L

L

R

R

G
|
G

L

R

G
|
G

G

G

A
|
A

G

G

F

F

P

P

A

S

G

G

R

L

K
|
K

W

W

R

S

I

F

V

M

R

N

-

P

-

G

-

W

-

E

D

K

Q

N

P

E

A

G

P

P

R

R

L

Y

M

L

A

V

V

V

N
|
N

I

A

D
|
D

E
|
E

G
|
G

E

E

P

P

G

G

T

T

F

C

K

K

D

D

R

R

T

E

Y

I

L

M

E

R

R

K

D

D

P

P

H

H

R

K

F

L

L

V

E

E

G

G

V

C

L

L

I

L

A

A

A

G

Q

R

V

A

V

M

G

N

T

A

E

T

A

A

I

A

Y

Y

I

I

Y

Y

L

I

R

R

D

G

E

E

Y

F

H

Y

G

N

C

E

R

A

V

A

L

V

L

L

Q

Q

Q

T

A

A

L

I

D

N

D

E

-

A

-

Y

-

A

-

G

L

L

R

I

A

G

E

K

P

D

P

A

C

C

P

G

L

S

P

G

H

Y

-

D

-

F

-

D

I

V

E

Y

L

I

R

H

R

R

G

G

A

M

G

G

A

A

Y
|
Y

I
x
V

C

C

G
|
G

E
|
E

E

E

S

T

A

S

M

L

I

I

E

E

S

S

I

L

E

E

G

G

K

K

R

A

G

G

E

K

P
|
P

R

R

M

L

R

K

P

P

Y

F

I

P

A

A

Q

G

V

V

G

G

L

L

F

F

G

G

R

R

P

P

T

S

L

T

E
x
V

H
x
T

N
|
N

F

V

E

E

T

T

L

V

Y

A

W

V

V

A

R

P

D

T

I

I

V

L

E

R

R

R

G

G

P

G

Q

D

W

W

F

F

A

A

S

S

F

F

G

G

R

R

H

E

G

R

R

N

K

S

G

G

L

T

R

K

S

L

F

F

S

C

V

I

S

S

G

G

R

N

V

V

K

N

H

E

P

P

G

C

V

T

K

V

L

E

A

E

P

E

A

M

G

S

I

I

T

P

V

L

Q

R

E

E

L

L

I

L

D

E

E

K

Y

H

C

C

G

G

L

I

L

K

D

G

G

G

-

W

H

D

Q

N

L

L

Y

L

A

G

Y

V

L

I

P

P

G

G

A

C

S

S

G

V

G

P

I

I

L

L

P

P

A

K

S

N

L

I

-

C

N

E

Q

D

I

V

P

L

L

M

D

D

F

F

D

D

T

A

L

L

Q

K

P

D

Y

V

G

Q

C

S

F

G

I

L

G

G

S

T

A

A

V

V

I

I

V

V

L

I

S

N

Q

K

H

Q

D

Q

R

D

A

V

R

I

D

R

A

A

A

I

L

Q

N

R

V

F

M

A

R

A

F

F

F

Y

E

K

H

H

E

E

S
|
S

C
|
C

G
|
G

Q
|
Q

C
|
C

T

T

P

P

C
|
C

R

R

V

E

G

G

T

T

A

T

K

W

A

L

T

K

L

A

M

M

-

D

-

R

-

F

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R

-

T

-

G

E

Q

A

A

P

K

Q

E

W

R

D

E

G

I

D

D

L

M

L

L

D

Y

D

E

L

L

A

T

Q

K

V

D

M

I

G

E

D

G

A

H

S
x
T

I

I

C
|
C

G
x
A

L
|
L

G

G

Q

D

A

A

P

A

N

W

P

P

I

I

R

Q

C

G

I

L

H

I

K

R

Y

N

F

F

P

R

H

P

E

E

V

M

binding flavin mononucleotide: G58 (= G267), G60 (= G269), A62 (= A271), K69 (= K278), N90 (= N294), D92 (= D296), E93 (= E297), G94 (= G298), Y178 (= Y374), V179 (≠ I375), G181 (= G377), E182 (= E378), V216 (≠ E412), T217 (≠ H413), N218 (= N414), A400 (≠ G588), L401 (= L589)
binding iron/sulfur cluster: P197 (= P393), S352 (= S546), C353 (= C547), G354 (= G548), Q355 (= Q549), C356 (= C550), C359 (= C553), T397 (≠ S585), C399 (= C587), L401 (= L589)

8b9zF Drosophila melanogaster complex i in the active state (dm1) (see paper)
39% identity, 58% coverage: 240:608/635 of query aligns to 45:428/441 of 8b9zF

query
sites
8b9zF

Y

Y

Q

K

T

T

A

K

A

E

A

I

L

V

V

L

N

K

G

G

E

-

M

-

D

-

A

A

E

D

A

W

V

I

L

V

A

N

A

E

M

I

E

K

D

T

S

S

G

G

L

L

R

R

G
|
G

L
x
R

G
|
G

G

G

A
|
A

G

G

F

F

P

P

A

S

G

G

R

M

K
|
K

W

W

R

S

I

F

V

M

R

-

D

N

Q

K

P

P

A

G

-

D

-

G

-

R

P

P

R

K

L

Y

M

L

A

V

V

V

N
|
N

I

A

D
|
D

E
|
E

G

G

E

E

P

P

G

G

T

T

F

C

K

K

D

D

R

R

T

E

Y

I

L

M

E

R

R

H

D

D

P

P

H

H

R

K

F

L

L

V

E

E

G

G

V

C

L

L

I

I

A

A

A

G

Q

R

V

A

V

M

G

G

T

A

E

Q

A

A

I

A

Y

Y

I

I

Y

Y

L

I

R

R

D

G

E

E

Y

F

H

Y

G

N

C

E

R

A

V

S

L

N

L

M

Q

Q

Q

L

A

A

L

I

D

A

D

E

-

A

-

Y

-

Q

-

A

-

G

L

L

R

I

A

G

E

K

P

N

P

A

C

C

P

G

L

T

P

G

H

Y

-

D

-

F

-

D

I

V

E

F

L

M

R

H

R

R

G

G

A

A

G

G

A

A

Y
|
Y

I
|
I

C

C

G
|
G

E
|
E

S

T

A

A

M

L

I

I

E

E

S

S

I

L

E

E

G

G

K

K

R

Q

G

G

E

K

P
|
P

R

R

M

L

R

K

P

P

Y

F

I

P

A

A

Q

D

V

V

G

G

L

V

F

F

G

G

R

C

P

P

T

T

L

T

E
x
V

H
x
T

N
|
N

F

V

E

E

T
|
T

L

V

Y

A

W

V

V

A

R

P

D

T

I

I

V

C

E

R

R

R

G

G

P

G

Q

V

W

W

F

F

A

A

S

S

F

F

G

G

R

R

H

T

G

R

R

N

K

S

G

G

L

T

R

K

S

L

F

F

S

N

V

I

S

S

G

G

R

H

V

V

K

N

H

R

P

P

G

C

V

T

K

V

L

E

A

E

P

E

A

M

G

S

I

I

T

P

V

L

Q

K

E

E

L

L

I

I

D

E

E

R

Y

H

C

C

G

G

L

V

L

T

D

G

G

G

-

W

H

D

Q

N

L

L

Y

L

A

G

Y

V

L

I

P

P

G

G

A

S

S

S

G

T

G

P

I

I

L

I

P

P

A

K

S

N

L

V

-

C

N

D

Q

D

I

V

P

I

L

M

D

D

F

F

D

D

T

G

L

L

Q

I

P

A

Y

A

G

Q

C

T

F

S

I

L

G

G

S

T

A

A

V

I

I

I

V

V

L

M

S

D

Q

K

H

S

D

T

R

D

A

V

R

I

D

K

A

A

A

I

L

A

N

R

V

L

M

I

R

S

F

F

F

Y

E

K

H

H

E

E

S
|
S

C
|
C

G
|
G

Q
|
Q

C
|
C

T

T

P

P

C
|
C

R

R

V

E

G

G

T

I

A

G

K

W

A

M

A

N

T

K

L

I

M

M

-

T

-

R

-

F

-

V

-

K

-

G

E

D

A

A

P

Q

Q

P

W

A

D

E

G

I

D

D

L

M

L

L

D

W

D

E

L

I

A

S

Q

K

V

Q

M

I

G

E

D

G

A

H

S
x
T

I
|
I

C
|
C

G
x
A

L
|
L

G
|
G

Q

D

A

G

A

A

P

A

N

W

P

P

I

V

R

Q

C

G

I

L

H

I

K

R

Y

H

F

F

P

R

H

P

E

E

V

I

binding flavin mononucleotide: G69 (= G267), R70 (≠ L268), G71 (= G269), A73 (= A271), K80 (= K278), N98 (= N294), D100 (= D296), E101 (= E297), Y186 (= Y374), G189 (= G377), E190 (= E378), E191 (= E379), V224 (≠ E412), T225 (≠ H413), N226 (= N414), T229 (= T417), A408 (≠ G588), L409 (= L589)
binding iron/sulfur cluster: I187 (= I375), P205 (= P393), S360 (= S546), C361 (= C547), G362 (= G548), Q363 (= Q549), C364 (= C550), C367 (= C553), T405 (≠ S585), I406 (= I586), C407 (= C587), L409 (= L589), G410 (= G590)

8eswV1 NADH dehydrogenase (Ubiquinone) 24 kDa subunit, isoform A (see paper)
39% identity, 58% coverage: 240:608/635 of query aligns to 43:426/439 of 8eswV1

query
sites
8eswV1

Y

Y

Q

K

T

T

A

K

A

E

A

I

L

V

V

L

N

K

G

G

E

-

M

-

D

-

A

A

E

D

A

W

V

I

L

V

A

N

A

E

M

I

E

K

D

T

S

S

G

G

L

L

R

R

G
|
G

L
x
R

G
|
G

G

G

A

A

G

G

F

F

P

P

A

S

G

G

R

M

K
|
K

W

W

R

S

I

F

V

M

R

-

D

N

Q

K

P

P

A

G

-

D

-

G

-

R

P

P

R

K

L

Y

M

L

A

V

V

V

N
|
N

I

A

D
|
D

E
|
E

G

G

E

E

P

P

G

G

T

T

F

C

K

K

D

D

R

R

T

E

Y

I

L

M

E

R

R

H

D

D

P

P

H

H

R

K

F

L

L

V

E

E

G

G

V

C

L

L

I

I

A

A

A

G

Q

R

V

A

V

M

G

G

T

A

E

Q

A

A

I

A

Y

Y

I

I

Y

Y

L

I

R

R

D

G

E

E

Y

F

H
x
Y

G
x
N

C

E

R

A

V
x
S

L
x
N

L

M

Q
|
Q

Q

L

A

A

L

I

D

A

D

E

-

A

-
x
Y

-

Q

-

A